Journal of microbiological methods最新文献_第2页

Machine learning powered profiling: Rapid identification of Klebsiella Pneumoniae drug resistance from MALDI-TOF MS 机器学习驱动的分析：利用MALDI-TOF质谱快速鉴定肺炎克雷伯菌耐药性。

IF 1.9 4区生物学

Journal of microbiological methods Pub Date : 2025-10-10 DOI: 10.1016/j.mimet.2025.107291

Xiaobo Xu, Yuntao Gao

{"title":"Machine learning powered profiling: Rapid identification of Klebsiella Pneumoniae drug resistance from MALDI-TOF MS","authors":"Xiaobo Xu, Yuntao Gao","doi":"10.1016/j.mimet.2025.107291","DOIUrl":"10.1016/j.mimet.2025.107291","url":null,"abstract":"<div><div>The early identification of drug-resistant phenotypes in <em>Klebsiella pneumoniae</em> is essential for effective clinical intervention, infection management, and the prevention of resistance development and spread. This study aimed to construct multiple machine-learning models to rapidly and comprehensively predict susceptibility to nine common antimicrobial drugs using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) data of <em>K. pneumoniae</em>. A total of 484 <em>K. pneumoniae</em> isolates from Zhejiang Rongjun Hospital were collected and tested for in vitro susceptibility to nine antibiotics. Six supervised learning models were developed and evaluated: Random Forest, eXtreme Gradient Boosting (XGBoost), Adaptive Boosting (AdaBoost), Logistic Regression, Multilayer Perceptron, and Support Vector Machine. Performance was assessed using test score, ten-fold cross-validation, accuracy, specificity, F1 score, AUROC, and 95 %CI. The models performed best for Amikacin and Co-trimoxazole, whilst exhibiting the poorest predictive efficacy for levofloxacin. AdaBoost and XGBoost achieved high predictive performance with AUROC values ≥0.8 for all nine antimicrobial drugs. The XGBoost model demonstrated strong performance and stability across evaluation metrics. SHAP analysis based on the XGBoost model identified key features such as 4517.5 ± 2.5 Da for Amikacin and 6022.5 ± 2.5 Da for Co-trimoxazole. The study concluded that analyzing MALDI-TOF MS data with machine-learning models can rapidly predict the antibiotic susceptibility of <em>K. pneumoniae</em>, reducing resistance detection time to 1–2 h and accelerating clinical decision-making.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"238 ","pages":"Article 107291"},"PeriodicalIF":1.9,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145280413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multienzyme isothermal rapid amplification (MIRA)-LFD assay for rapid detection of PEDV and PoRVA 多酶等温快速扩增(MIRA)-LFD法快速检测PEDV和PoRVA。

IF 1.9 4区生物学

Journal of microbiological methods Pub Date : 2025-10-10 DOI: 10.1016/j.mimet.2025.107289

Yicong Fan , Jianxin Wang , Xianyu Bian , Wei Wang , Jinzhu Zhou , Xuehan Zhang , Mi Hu , Min Sun , Shanshan Yang , Yongxiang Zhao , Baochao Fan , Bin Li , Junming Zhou

{"title":"Multienzyme isothermal rapid amplification (MIRA)-LFD assay for rapid detection of PEDV and PoRVA","authors":"Yicong Fan , Jianxin Wang , Xianyu Bian , Wei Wang , Jinzhu Zhou , Xuehan Zhang , Mi Hu , Min Sun , Shanshan Yang , Yongxiang Zhao , Baochao Fan , Bin Li , Junming Zhou","doi":"10.1016/j.mimet.2025.107289","DOIUrl":"10.1016/j.mimet.2025.107289","url":null,"abstract":"<div><div>Porcine epidemic diarrhea virus (PEDV) and porcine rotavirus A (PoRVA), as primary pathogens of viral diarrhea in piglets, lead to substantial economic losses in global swine production. Existing detection approaches—including virus isolation, ELISA, and PCR—are limited by prolonged turnaround times, dependence on specialized equipment, or low sensitivity, which hinder their utility for rapid on-site diagnosis. In this study, we established RT-MIRA-LFD, a rapid, sensitive, and visually interpretable on-site detection platform for PEDV and PoRVA, leveraging multienzyme isothermal rapid amplification (MIRA) and lateral flow dipstick (LFD). Forward and reverse primers targeting conserved regions of the PEDV N and PoRVA VP6 genes were designed with 5′-6-FAM and 5′-biotin modifications, respectively, enabling seamless integration of MIRA amplification and LFD readout. Sensitivity testing revealed detection limits of 20 copies/μL (PEDV) and 4.5 copies/μL (PoRVA) for plasmid standards. Both assays showed no cross-reactivity with TGEV, PDCoV, CSFV, or other common swine pathogens, confirming high specificity. Clinical validation with 50 anal swab samples demonstrated 98 % (PEDV) and 96 % (PoRVA) concordance with RT-qPCR, supported by Kappa values of 0.92 and 0.96. The entire workflow completes within 30 min, bypasses complex instrumentation, and allows direct visual interpretation, dramatically enhancing field-deployable detection efficiency. This RT-MIRA-LFD platform offers a practical solution for early diagnosis, outbreak monitoring, and targeted control of PEDV and PoRVA, especially in resource-limited settings, thereby supporting efforts to reduce economic impacts on swine operations.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"238 ","pages":"Article 107289"},"PeriodicalIF":1.9,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145274716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enterobacteriaceae in unpackaged ice cream: Implications for microbial safety and methodological concordance between ISO and TEMPO® 未包装冰淇淋中的肠杆菌科：ISO和TEMPO®之间的微生物安全性和方法学一致性的含义。

IF 1.9 4区生物学

Journal of microbiological methods Pub Date : 2025-10-05 DOI: 10.1016/j.mimet.2025.107287

Adem Soycan, Mustafa Bilgin

{"title":"Enterobacteriaceae in unpackaged ice cream: Implications for microbial safety and methodological concordance between ISO and TEMPO®","authors":"Adem Soycan, Mustafa Bilgin","doi":"10.1016/j.mimet.2025.107287","DOIUrl":"10.1016/j.mimet.2025.107287","url":null,"abstract":"<div><div>Ice cream, a widely consumed dairy product, especially during summer, can pose serious microbiological risks when produced and sold under unhygienic conditions. This study evaluated the microbiological quality of unpackaged plain ice creams sold in the Kocaeli region of Türkiye between April and September 2024, focusing on <em>Enterobacteriaceae</em> contamination and comparing two enumeration methods: the ISO 21528-2 standard and the TEMPO® system. A total of 118 samples were analyzed. <em>Enterobacteriaceae</em> were detected in 41.5 % of samples, with mean counts around 2.3 log CFU/g by both methods. No statistically significant difference was observed between methods (<em>p</em> > 0.05), confirming the TEMPO® system as a rapid, reliable, and ISO-compliant alternative for routine monitoring. <em>Staphylococcus aureus</em> was isolated in 4.2 % of samples, with a mean count of 2.67 ± 0.15 log CFU/g, while <em>Salmonella</em> spp. and <em>Listeria monocytogenes</em> were not detected, suggesting effective pathogen control likely due to pasteurization and hygiene measures. The high prevalence of <em>Enterobacteriaceae</em> indicates lapses in hygiene during production and vending, posing a risk to public health. This is the first study in Türkiye to validate the TEMPO® EB system against ISO 21528-2 in unpackaged ice cream, demonstrating both excellent concordance and operational advantages. These findings underscore the critical need for stringent hygiene practices, cold chain maintenance, and HACCP implementation in street-vended ice cream production to ensure consumer safety.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"238 ","pages":"Article 107287"},"PeriodicalIF":1.9,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145244566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification of optimally produced extracellular fungal lipases and their role in the remediation of oil-rich kitchen wastewater 最佳产胞外真菌脂肪酶的纯化及其在富油厨房废水修复中的作用。

IF 1.9 4区生物学

Journal of microbiological methods Pub Date : 2025-10-02 DOI: 10.1016/j.mimet.2025.107288

Ayesha Safdar , Fatima Ismail , Muhammad Imran , Muhammad Anjum Zia , Shazia Parveen , Tahmina Bibi

{"title":"Purification of optimally produced extracellular fungal lipases and their role in the remediation of oil-rich kitchen wastewater","authors":"Ayesha Safdar , Fatima Ismail , Muhammad Imran , Muhammad Anjum Zia , Shazia Parveen , Tahmina Bibi","doi":"10.1016/j.mimet.2025.107288","DOIUrl":"10.1016/j.mimet.2025.107288","url":null,"abstract":"<div><div>Wastewater containing oils and lipids can have major environmental consequences, such as water contamination, soil erosion, and harmful impacts on ecosystems and aquatic life. Therefore, to eliminate oils and fix these discharges for the benefit of the environment, proper treatment of wastewater is essential. In this regard, lipases can be applied to a variety of domestic and commercial wastewater biotreatment applications. Lipases in the current study were produced using <em>A. niger</em> (MG654699.1), <em>C. albicans</em> (ATCC10231), and <em>A</em>. <em>sclerotigenum</em> (MK732096.1). The SSF optimization revealed that the highest quantity of lipase was generated for selected strains at 30 °C along with an optimized size of inoculum and moisture content, i.e., 5 mL, and at pH 7 in the case of <em>A. niger</em> and <em>C. albicans</em> and pH 8 in the case of <em>A</em>. <em>sclerotigenum</em>. The <em>A. niger</em> lipase after purification showed a specific activity of 42.7 Umg<sup>−1</sup>, fold purification of 2.29, percentage yield of 57.5 %, and molecular weight of 30 kDa. The <em>C. albicans</em> purified lipase exhibited a specific activity of 35.6 Umg<sup>−1</sup>, a fold purification of 2.42, a percentage yield of 56.4 %, and a molecular weight of 45 kDa. The purified lipase from <em>A</em>. <em>sclerotigenum</em> showed a specific activity of 38.5 Umg<sup>−1</sup>, a fold purification of 4.35, a percentage yield of 57.7 %, and a molecular weight of 80 kDa. The degradation of oil-rich wastewater by fungal lipases was confirmed by the FTIR analysis of the control and lipase-treated kitchen wastewater samples. The results showed that these fungal lipases could be used in the bioremediation of wastewater with significant amounts of oils and lipids.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"238 ","pages":"Article 107288"},"PeriodicalIF":1.9,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145228398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predictive value of IL-6, IL-1β, TNF-α, and vaginal pH in diagnosing vaginal microbial infections: A host-inflammatory axis perspective. IL-6、IL-1β、TNF-α和阴道pH值在诊断阴道微生物感染中的预测价值：宿主-炎症轴视角

IF 1.9 4区生物学

Journal of microbiological methods Pub Date : 2025-10-01 Epub Date: 2025-08-06 DOI: 10.1016/j.mimet.2025.107212

Kiran Kharsodiya, Kaushik Pratim Das, Harish Kumar Dhingra

{"title":"Predictive value of IL-6, IL-1β, TNF-α, and vaginal pH in diagnosing vaginal microbial infections: A host-inflammatory axis perspective.","authors":"Kiran Kharsodiya, Kaushik Pratim Das, Harish Kumar Dhingra","doi":"10.1016/j.mimet.2025.107212","DOIUrl":"10.1016/j.mimet.2025.107212","url":null,"abstract":"<p><p>Microbial-associated vaginal infections are common among women of reproductive age and are linked to alterations in the local immune environment. Inflammatory biomarkers such as IL-6, IL-β, and TNF-α, along with vaginal pH have emerged as potential indicators of microbial dysbiosis. This study aimed to statistically evaluate the ability of these specific inflammatory cytokines and vaginal pH to identify infection status. Cytokine concentrations and vaginal pH were measured in clinically characterized samples. The group differences were analyzed using Mann-Whitney U tests and Cliff's Delta for effect size. ROC-AUC analysis was also performed to assess the discriminative power, and correlation heatmaps explored marker synergy. The infected individuals showed increased levels of all cytokines (p < 0.001), with large effect size (Δ > 0.9 for IL-6, IL-1β, TNF-α). Vaginal pH also differed significantly (Δ = 0.60). In addition, the combination of IL-6 and vaginal pH achieved excellent discriminative performance (AUC = 0.98). These findings suggest that IL-6, IL-1β, and TNF-α, when combined with vaginal pH, can function as reliable non-invasive biomarkers for the early detection and improved diagnostic triaging of vaginal microbial infections.</p>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":" ","pages":"107212"},"PeriodicalIF":1.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiotic protection assay revisited: Metronidazole is unable to completely eliminate Porphyromonas gingivalis. 抗生素保护试验再访：甲硝唑不能完全消除牙龈卟啉单胞菌。

IF 1.9 4区生物学

Journal of microbiological methods Pub Date : 2025-10-01 Epub Date: 2025-08-06 DOI: 10.1016/j.mimet.2025.107214

Caroline A de Jongh, Laura Volle, Chen Sun, Dongmei Deng, Michel A Hoogenkamp, Kamran Nazmi, Floris J Bikker, Bastiaan P Krom

{"title":"Antibiotic protection assay revisited: Metronidazole is unable to completely eliminate Porphyromonas gingivalis.","authors":"Caroline A de Jongh, Laura Volle, Chen Sun, Dongmei Deng, Michel A Hoogenkamp, Kamran Nazmi, Floris J Bikker, Bastiaan P Krom","doi":"10.1016/j.mimet.2025.107214","DOIUrl":"10.1016/j.mimet.2025.107214","url":null,"abstract":"<p><p>Intracellular bacterial survival is widely studied in host-microbiome interactions. The antibiotic protection assay is often used to quantify intracellular bacteria. This method uses gentamicin to kill extracellular bacteria, where the bacteria that remain inside host cells survive the treatment. However, gentamicin is ineffective against anaerobic bacteria, such as Porphyromonas gingivalis. To remedy this, metronidazole is often incorporated. However, the effectiveness of this adaptation seems not to be validated properly. The aim of this study was to show the ineffectiveness of metronidazole to eliminate extracellular P. gingivalis in vitro. Microscopy showed uptake of P. gingivalis by murine J774A.1 macrophages and primary human macrophages. However, quantification of intracellular bacteria was unreliable as the control without macrophages contained significant numbers of viable bacteria. Upon testing metronidazole under assay conditions, P. gingivalis survived within the tested timeframes. Next, it was attempted to find a suitable alternative antibiotic compound to use in the antibiotic protection assay. The MIC and MBC were therefore determined for various alternative antibiotics and antimicrobial peptides. None of the included antibiotics effectively killed P. gingivalis. Antimicrobial peptides cycloLL-37 and D-LL-31 were effective against P. gingivalis, but were toxic to macrophages under the conditions used as determined using a Lactate dehydrogenase-based cytotoxicity assay. To conclude, metronidazole and gentamicin seem unsuitable for the antibiotic protection assay for the strict anaerobic oral bacterium P. gingivalis. To be able to determine viable intracellular P. gingivalis, alternative bactericidal agents should be found which, under assay conditions, eliminate extracellular P. gingivalis without entering or affecting mammalian cells.</p>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":" ","pages":"107214"},"PeriodicalIF":1.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coxiella burnetii: Emerging threats, molecular insights, and advances in diagnosis and control measures. 伯纳氏杆菌：新出现的威胁，分子的见解和诊断和控制措施的进展。

IF 1.9 4区生物学

Journal of microbiological methods Pub Date : 2025-10-01 Epub Date: 2025-08-06 DOI: 10.1016/j.mimet.2025.107213

Mohammad Reza Mohammadi, Safoura Moradkasani, Mina Latifian, Saber Esmaeili

{"title":"Coxiella burnetii: Emerging threats, molecular insights, and advances in diagnosis and control measures.","authors":"Mohammad Reza Mohammadi, Safoura Moradkasani, Mina Latifian, Saber Esmaeili","doi":"10.1016/j.mimet.2025.107213","DOIUrl":"10.1016/j.mimet.2025.107213","url":null,"abstract":"<p><p>Coxiella burnetii, a Gram-negative, obligate intracellular bacterium and causative agent of Q fever, is a re-emerging zoonotic pathogen with a complex transmission cycle involving livestock (cattle, sheep, and goats), diverse terrestrial and aquatic wildlife, arthropod vectors (ticks and fleas), and resilient environmental reservoirs, such as free-living amoebae. Humans are mainly infected by inhaling contaminated aerosols, especially during parturition. This review offers an integrative synthesis of current research across six key domains: ecological reservoirs, diagnostic strategies, molecular epidemiology, therapeutic challenges, vaccine development, and the One Health approach. We first examined emerging insights into host and vector diversity, including underexplored aquatic and semi-aquatic species, and environmental factors sustaining endemicity. We then assessed recent diagnostic innovations, such as multiplex and digital PCR, LAMP, metagenomic sequencing, and immunohistochemistry, alongside conventional serological tools, such as ELISA and IFA. Given the taxonomic complexity introduced by genetically related Coxiella-like endosymbionts, we highlight the necessity of high-resolution molecular typing platforms, such as MLVA, MST, and SNP analysis, for accurate strain discrimination. In clinical and environmental contexts, sample matrices now include blood, milk, feces, urine, respiratory secretions, and ectoparasites, enabling more sensitive surveillance. Despite this progress, Q fever control remains challenging because of nonspecific symptoms, diagnostic delays, chronic complications, and reliance on prolonged antibiotic therapy. Advances in antimicrobial testing and evolving vaccine strategies offer hope; however, durable cross-strain protection remains elusive. Adopting a One Health approach, this review highlights the key knowledge gaps and strategic priorities for reducing the global burden of C. burnetii across human, animal, and environmental health sectors.</p>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":" ","pages":"107213"},"PeriodicalIF":1.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Statistical optimization of amylase production from Bacillus amyloliquefaciens using agro-industrial waste (mango peels) under submerged fermentation by response surface methodology. 利用工农业废弃物（芒果皮）深层发酵解淀粉芽孢杆菌生产淀粉酶的响应面法统计优化

IF 1.9 4区生物学

Journal of microbiological methods Pub Date : 2025-10-01 Epub Date: 2025-08-07 DOI: 10.1016/j.mimet.2025.107208

Saba Saeed, Hafiz Abdullah Shakir, Javed Iqbal Qazi

{"title":"Statistical optimization of amylase production from Bacillus amyloliquefaciens using agro-industrial waste (mango peels) under submerged fermentation by response surface methodology.","authors":"Saba Saeed, Hafiz Abdullah Shakir, Javed Iqbal Qazi","doi":"10.1016/j.mimet.2025.107208","DOIUrl":"10.1016/j.mimet.2025.107208","url":null,"abstract":"<p><p>The current study aimed to optimize the amylase producing strain using agro-industrial waste under submerged fermentation. Of six bacterial strains, five gave positive results for amylase production but Bacillus amyloliquefaciens was selected due to highest enzyme production. The medium components were selected using Plackett-Burman design (PBD) while concentration of medium components was determined using the central composite design (CCD) of response surface methodology (RSM). The maximum enzyme production was obtained from mango peels (1.25 %), sodium chloride (0.15 %), potassium nitrate (0.06 %), and magnesium sulfate (0.03 %). It was observed that B. amyloliquefaciens produce maximum amylase at 45 °C, pH 5, and 2 % inoculum size over 24-h incubation period. Furthermore, partial characterization revealed best enzyme activity at 9 pH, 50 °C, 1 % substrate concentration, and 30 min of incubation. Besides, amylase was found more stable at 6 pH and 60 ᴼC. In the presence of metal ions, the enzyme activity decreased by Cu<sup>+2</sup> (5 mM) and found maximum in Ca<sup>+2</sup> (1 mM). With the increase in concentration of inhibitors, the amylase activity of ethylenediaminetetraacetic acid (EDTA) and 1, 10-phenanthroline decreases. Among organic solvents, surfactants, and oxidizing agents maximum activity was observed for sodium dodecylsulphate (SDS) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). These findings highlight the potential of B. amyloliquefaciens as a promising candidate for sustainable and cost-effective industrial amylase production using agro-industrial waste and can be further explored for scale-up studies.</p>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":" ","pages":"107208"},"PeriodicalIF":1.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144775587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Appropriate swab method for monitoring norovirus on environmental surfaces in an urban hospital 城市医院环境表面诺如病毒监测的适宜拭子法。

IF 1.9 4区生物学

Journal of microbiological methods Pub Date : 2025-09-30 DOI: 10.1016/j.mimet.2025.107286

Uraporn Phumisantiphong , Thanwa Wongsuk , Anan Manomaipiboon , Suwat Suksawasdi , Phensiri Klaewkasikij , Dolruthai Boonchoo , Phimphitcha Prayoonwong , Achiraya Prajongsai , Siriporn Sriwilai , Warissara Jirawathin , Kitwadee Rupprom

{"title":"Appropriate swab method for monitoring norovirus on environmental surfaces in an urban hospital","authors":"Uraporn Phumisantiphong , Thanwa Wongsuk , Anan Manomaipiboon , Suwat Suksawasdi , Phensiri Klaewkasikij , Dolruthai Boonchoo , Phimphitcha Prayoonwong , Achiraya Prajongsai , Siriporn Sriwilai , Warissara Jirawathin , Kitwadee Rupprom","doi":"10.1016/j.mimet.2025.107286","DOIUrl":"10.1016/j.mimet.2025.107286","url":null,"abstract":"<div><div>Environmental surface contamination leads to microbial transmission. Norovirus is a leading cause of acute nonbacterial gastroenteritis. The virus remains stable in various environments and spreads through multiple routes, including direct contact with surfaces. This study aims to apply the swab method to detect norovirus on environmental surfaces in an urban hospital. Sterile distilled water used as the swab wetting buffer provided the highest recovery rate for norovirus (101.3 % ± 22.7 %). The norovirus GII concentration recovered from environmental surfaces ranged from 10<sup>4</sup> to 10<sup>6</sup> genome copies/mL, with average recovery rates of 112.1 % ± 45.4 % for polystyrene, 66.0 % ± 47.1 % for stainless steel, and 40.6 % ± 15.6 % for ceramic. The presence of norovirus on 210 environmental surfaces surrounding hospitalized patients was determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Subsequent RT-nested PCR revealed positivity for norovirus GI (GI.3) in only 1 of 210 samples. Therefore, the swab test technique should be considered for routine monitoring for norovirus contamination on hospital surfaces. The results of this study can help guide the control and prevention of infectious diseases in hospitals.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"238 ","pages":"Article 107286"},"PeriodicalIF":1.9,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145212844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomass and metabolic activity staining biofilm techniques are not reliable enough to be used in microbiology laboratories 生物质和代谢活性染色生物膜技术是不够可靠的，用于微生物实验室。

IF 1.9 4区生物学

Journal of microbiological methods Pub Date : 2025-09-29 DOI: 10.1016/j.mimet.2025.107285

Marta Díaz-Navarro , Ana Crespo , Patricia Muñoz , María Guembe

引用次数: 0