城市医院环境表面诺如病毒监测的适宜拭子法。

IF 1.9 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2025-09-30 DOI:10.1016/j.mimet.2025.107286

Uraporn Phumisantiphong , Thanwa Wongsuk , Anan Manomaipiboon , Suwat Suksawasdi , Phensiri Klaewkasikij , Dolruthai Boonchoo , Phimphitcha Prayoonwong , Achiraya Prajongsai , Siriporn Sriwilai , Warissara Jirawathin , Kitwadee Rupprom

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引用次数: 0

摘要

环境表面污染导致微生物传播。诺如病毒是急性非细菌性胃肠炎的主要病因。该病毒在各种环境中保持稳定，并通过多种途径传播，包括与物体表面直接接触。本研究旨在应用棉签法检测城市医院环境表面的诺如病毒。无菌蒸馏水作为棉签润湿缓冲液，诺如病毒回收率最高（101.3 % ± 22.7 %）。诺瓦克病毒的GII从环境中恢复过来的表面浓度范围从104年到106年基因组拷贝/毫升,平均回收率为112.1 % ±45.4  %为聚苯乙烯, % 66.0±47.1  %为不锈钢,和40.6 % ±15.6  %为陶瓷。采用逆转录-定量聚合酶链反应（RT-qPCR）检测了210例住院患者周围环境表面上诺瓦克病毒的存在。随后的rt -巢式PCR显示，210份样本中只有1份诺如病毒GI （GI.3）阳性。因此，应考虑使用拭子试验技术对医院表面的诺如病毒污染进行常规监测。本研究结果可指导医院传染病的控制与预防。

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Appropriate swab method for monitoring norovirus on environmental surfaces in an urban hospital

Environmental surface contamination leads to microbial transmission. Norovirus is a leading cause of acute nonbacterial gastroenteritis. The virus remains stable in various environments and spreads through multiple routes, including direct contact with surfaces. This study aims to apply the swab method to detect norovirus on environmental surfaces in an urban hospital. Sterile distilled water used as the swab wetting buffer provided the highest recovery rate for norovirus (101.3 % ± 22.7 %). The norovirus GII concentration recovered from environmental surfaces ranged from 10⁴ to 10⁶ genome copies/mL, with average recovery rates of 112.1 % ± 45.4 % for polystyrene, 66.0 % ± 47.1 % for stainless steel, and 40.6 % ± 15.6 % for ceramic. The presence of norovirus on 210 environmental surfaces surrounding hospitalized patients was determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Subsequent RT-nested PCR revealed positivity for norovirus GI (GI.3) in only 1 of 210 samples. Therefore, the swab test technique should be considered for routine monitoring for norovirus contamination on hospital surfaces. The results of this study can help guide the control and prevention of infectious diseases in hospitals.

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.