Biomass and metabolic activity staining biofilm techniques are not reliable enough to be used in microbiology laboratories

IF 1.9 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2025-09-29 DOI:10.1016/j.mimet.2025.107285

Marta Díaz-Navarro , Ana Crespo , Patricia Muñoz , María Guembe

引用次数: 0

Abstract

Crystal violet and XTT staining quantify biofilms but are influenced by external factors. Testing MSSA, E. coli, and C. albicans refrigerated five weeks revealed weekly variability between researchers. These methods need careful interpretation and should be supported by more reproducible techniques for reliable results.

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生物质和代谢活性染色生物膜技术是不够可靠的，用于微生物实验室。

结晶紫和XTT染色定量生物膜，但受外界因素的影响。对冷冻五周的MSSA、大肠杆菌和白色念珠菌的检测显示，研究人员之间每周都存在差异。这些方法需要仔细解释，并应以更可重复的技术支持，以获得可靠的结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.