Elena Schreiber , Fritjof Freise , Nicole de Buhr , Isabel Hennig-Pauka
{"title":"肾上腺素对胸膜肺炎放线杆菌分离株生长特性的影响","authors":"Elena Schreiber , Fritjof Freise , Nicole de Buhr , Isabel Hennig-Pauka","doi":"10.1016/j.mimet.2025.107193","DOIUrl":null,"url":null,"abstract":"<div><div>Based on empirical observations, stressors in swine populations may contribute to outbreaks due to <em>Actinobacillus pleuropneumoniae</em> (<em>App</em>) in colonized pigs. Stress hormones as catecholamines were found to impact immune mechanism but also bacterial protein expression <em>in vivo</em>, so it can be hypothesized that they are key molecules in disease pathogenesis. Culturing methods to isolate <em>App</em> from tonsillar tissue of carrier pigs for further typing are mostly not successful. In this study the effect of epinephrine in culturing media on <em>App</em> growth behavior was tested. Starting cultures of different age (24-h-old or 7-day-old cultures) of three <em>App</em> field strains per serotypes 2, 5 and 12 were compared in their growth behavior in medium supplemented with various epinephrine concentrations. The response of <em>App</em> strains to epinephrine varied between strains. Highest differences in CFU/mL and in growth curve characteristics between different epinephrine concentrations were found in serotype 5 strains. Inflection points of bacterial growth curves with 24-h-old starting cultures were delayed with 100 μM epinephrine with exception of one ST5 strain. In all ST2 and ST12 as well as one ST5 strain 100 μM epinephrine led to a growth advantage in 7-day old compared to 24-h-old starting cultures. Epinephrine did not consistently enhance growth of <em>App</em>, so that it cannot be recommended as a supplement for growth media to facilitate isolation from colonized tonsils of subclinically infected pigs.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"236 ","pages":"Article 107193"},"PeriodicalIF":1.7000,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of epinephrine on growth characteristics of Actinobacillus pleuropneumoniae field isolates\",\"authors\":\"Elena Schreiber , Fritjof Freise , Nicole de Buhr , Isabel Hennig-Pauka\",\"doi\":\"10.1016/j.mimet.2025.107193\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Based on empirical observations, stressors in swine populations may contribute to outbreaks due to <em>Actinobacillus pleuropneumoniae</em> (<em>App</em>) in colonized pigs. Stress hormones as catecholamines were found to impact immune mechanism but also bacterial protein expression <em>in vivo</em>, so it can be hypothesized that they are key molecules in disease pathogenesis. Culturing methods to isolate <em>App</em> from tonsillar tissue of carrier pigs for further typing are mostly not successful. In this study the effect of epinephrine in culturing media on <em>App</em> growth behavior was tested. Starting cultures of different age (24-h-old or 7-day-old cultures) of three <em>App</em> field strains per serotypes 2, 5 and 12 were compared in their growth behavior in medium supplemented with various epinephrine concentrations. The response of <em>App</em> strains to epinephrine varied between strains. Highest differences in CFU/mL and in growth curve characteristics between different epinephrine concentrations were found in serotype 5 strains. Inflection points of bacterial growth curves with 24-h-old starting cultures were delayed with 100 μM epinephrine with exception of one ST5 strain. In all ST2 and ST12 as well as one ST5 strain 100 μM epinephrine led to a growth advantage in 7-day old compared to 24-h-old starting cultures. Epinephrine did not consistently enhance growth of <em>App</em>, so that it cannot be recommended as a supplement for growth media to facilitate isolation from colonized tonsils of subclinically infected pigs.</div></div>\",\"PeriodicalId\":16409,\"journal\":{\"name\":\"Journal of microbiological methods\",\"volume\":\"236 \",\"pages\":\"Article 107193\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2025-07-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of microbiological methods\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0167701225001095\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of microbiological methods","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0167701225001095","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Effect of epinephrine on growth characteristics of Actinobacillus pleuropneumoniae field isolates
Based on empirical observations, stressors in swine populations may contribute to outbreaks due to Actinobacillus pleuropneumoniae (App) in colonized pigs. Stress hormones as catecholamines were found to impact immune mechanism but also bacterial protein expression in vivo, so it can be hypothesized that they are key molecules in disease pathogenesis. Culturing methods to isolate App from tonsillar tissue of carrier pigs for further typing are mostly not successful. In this study the effect of epinephrine in culturing media on App growth behavior was tested. Starting cultures of different age (24-h-old or 7-day-old cultures) of three App field strains per serotypes 2, 5 and 12 were compared in their growth behavior in medium supplemented with various epinephrine concentrations. The response of App strains to epinephrine varied between strains. Highest differences in CFU/mL and in growth curve characteristics between different epinephrine concentrations were found in serotype 5 strains. Inflection points of bacterial growth curves with 24-h-old starting cultures were delayed with 100 μM epinephrine with exception of one ST5 strain. In all ST2 and ST12 as well as one ST5 strain 100 μM epinephrine led to a growth advantage in 7-day old compared to 24-h-old starting cultures. Epinephrine did not consistently enhance growth of App, so that it cannot be recommended as a supplement for growth media to facilitate isolation from colonized tonsils of subclinically infected pigs.
期刊介绍:
The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach.
All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.