Assessing the diagnostic utility of routine liquid enriched media in the processing of microbiological samples

IF 1.7 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2025-07-17 DOI:10.1016/j.mimet.2025.107196

Victoria Jordan , Hemalatha Varadhan

引用次数: 0

Abstract

A retrospective review of the utility of routinely inoculating thioglycollate broth for select sterile fluids was performed. The broth was contributory in ≤1 % of samples, prompting removal of broth inoculation from routine processing of these sample types. Laboratories should consider reviewing the routine use of enriched broth particularly in the context of modern ancillary methods of pathogen detection.

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评估常规液体富集培养基在微生物样品处理中的诊断效用。

回顾性审查的效用常规接种巯基乙酸酯肉汤选择无菌液体进行。肉汤在≤1 %的样品中起作用，提示在这些样品类型的常规处理中取消肉汤接种。实验室应考虑审查强化肉汤的常规使用，特别是在现代辅助病原体检测方法的背景下。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.