碳氟化合物溶剂可清除吲哚并促进大肠杆菌的生长。

IF 1.9 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2025-03-25 DOI:10.1016/j.mimet.2025.107123

Ryosuke Hosoki , Katsuaki Izawa , Kosuke Kawanaka , Yoshihisa Yamashige , Shojiro Kikuchi , Yuichi Ogawa , Masahiko Harata

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引用次数: 0

摘要

氟碳溶剂已被证明能促进细菌生长；然而，潜在的机制仍不清楚。在这里，我们表明这些溶剂清除细菌产生的吲哚，减少其对生长的抑制作用。这些发现可用于改进细菌培养方法和探索吲哚在细菌群落中的作用。

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Fluorocarbon solvent scavenges indole and promotes Escherichia coli growth

Fluorocarbon solvents have been shown to promote bacterial growth; however, the underlying mechanisms remain unclear. Here, we show that these solvents scavenge bacterially produced indole, reducing its inhibitory effect on growth. These findings can be utilized to improve bacterial culture methods and explore the roles of indole in bacterial communities.

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.