Journal of Nanobiotechnology最新文献

{"title":"Recombinant high-density lipoprotein targeted delivery of celastrol to promote foam cells lipophagy against early atherosclerosis.","authors":"Yang Li, Xiaoxia Xue, Liuchunyang Yu, Jinxiu Qian, Xiaoyu Li, Meng Tian, Jue Yang, Rongjun Deng, Cheng Lu, Cheng Xiao, Yuanyan Liu","doi":"10.1186/s12951-025-03327-9","DOIUrl":"10.1186/s12951-025-03327-9","url":null,"abstract":"<p><strong>Introduction: </strong>Atherosclerosis serving as the main underlying factor of cardiovascular disease (CVD) remains the primary cause of mortality and morbidity globally, while the deposition of massive cholesterol in macrophage-derived foam cells exerts pivotal roles in the occurrence and progression of atherosclerosis. Celastrol (CEL) is a bioactive ingredient owning potent capability to modulate lipid metabolism, whereas the poor bioavailability and potential toxicity limit its clinical application.</p><p><strong>Objectives: </strong>This study aims to design a CEL-loaded recombinant high-density lipoprotein (rHDL) delivery platform for active targeting, which may effectively promote lipid degradation in foam cells and reversely transport excessive cholesterol to the liver for metabolism in time.</p><p><strong>Methods: </strong>The rHDL loaded with CEL (CEL-rHDL) was prepared by the thin film dispersion method. Then the anti-atherosclerotic efficacy and targeted delivery to foam cells of atherosclerotic lesions were verified both in vitro and in vivo. RNA-sequence was applied to reveal the potential mechanism against early atherosclerosis, which was further validated through several molecular biology experiments.</p><p><strong>Results: </strong>The prepared CEL-rHDL increased the targeting efficiency to foam cells of atherosclerotic lesions, mitigated its off-target toxicity, and improved anti-atherosclerotic efficacy. Importantly, CEL-rHDL decreased lipid storage in foam cells by triggering lipophagy via the activation of Ca²⁺/CaMKKβ/AMPK/mTOR signaling pathway and reverse cholesterol transport (RCT).</p><p><strong>Conclusion: </strong>A combination of hypolipidemic chemo-intervention with rHDL participated specific and reverse delivery may offer a promising strategy for biocompatible treatment of early atherosclerosis.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"237"},"PeriodicalIF":10.6,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929195/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143677157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physical stimuli-responsive DNA hydrogels: design, fabrication strategies, and biomedical applications.","authors":"Rumi Acharya, Sayan Deb Dutta, Hemadri Mallik, Tejal V Patil, Keya Ganguly, Aayushi Randhawa, Hojin Kim, Jieun Lee, Hyeonseo Park, Changyeun Mo, Ki-Taek Lim","doi":"10.1186/s12951-025-03237-w","DOIUrl":"10.1186/s12951-025-03237-w","url":null,"abstract":"<p><p>Physical stimuli-responsive DNA hydrogels hold immense potential for tissue engineering due to their inherent biocompatibility, tunable properties, and capacity to replicate the mechanical environment of natural tissue, making physical stimuli-responsive DNA hydrogels a promising candidate for tissue engineering. These hydrogels can be tailored to respond to specific physical triggers such as temperature, light, magnetic fields, ultrasound, mechanical force, and electrical stimuli, allowing precise control over their behavior. By mimicking the extracellular matrix (ECM), DNA hydrogels provide structural support, biomechanical cues, and cell signaling essential for tissue regeneration. This article explores various physical stimuli and their incorporation into DNA hydrogels, including DNA self-assembly and hybrid DNA hydrogel methods. The aim is to demonstrate how DNA hydrogels, in conjunction with other biomolecules and the ECM environment, generate dynamic scaffolds that respond to physical stimuli to facilitate tissue regeneration. We investigate the most recent developments in cancer therapies, including injectable DNA hydrogel for bone regeneration, personalized scaffolds, and dynamic culture models for drug discovery. The study concludes by delineating the remaining obstacles and potential future orientations in the optimization of DNA hydrogel design for the regeneration and reconstruction of tissue. It also addresses strategies for surmounting current challenges and incorporating more sophisticated technologies, thereby facilitating the clinical translation of these innovative hydrogels.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"233"},"PeriodicalIF":10.6,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929200/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143677081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revolutionizing lung cancer treatment: harnessing exosomes as early diagnostic biomarkers, therapeutics and nano-delivery platforms.","authors":"Qiyao Xiao, Minhong Tan, Ge Yan, Lihua Peng","doi":"10.1186/s12951-025-03306-0","DOIUrl":"10.1186/s12951-025-03306-0","url":null,"abstract":"<p><p>Lung cancer, known for its high morbidity and mortality rates, remains one of the most critical health challenges globally. Conventional treatment options, such as chemotherapy and surgery, are often limited by high costs, significant side effects, and often yield a poor prognosis. Notably, recent research has shed light on the potential therapeutic roles of exosomes, which essentially influence lung cancer's development, diagnosis, treatment, and prognosis. Exosomes have been revealed for their exceptional properties, including natural intercellular communication, excellent biocompatibility, minimal toxicity, prolonged blood circulation ability, and biodegradability. These unique characteristics position exosomes as highly effective drug delivery systems, nanotherapeutics, and potential diagnostic and prognostic biomarkers in lung cancer. This review provides a comprehensive review of the physiological and pathological roles of exosomes in lung cancer, emphasizing their potential as innovative diagnostic biomarkers, therapeutics, and delivery platforms. By harnessing their unique properties, exosomes are poised to revolutionize the diagnosis and treatment of lung cancer, offering a promising avenue for more personalized and effective therapies.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"232"},"PeriodicalIF":10.6,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929271/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143677158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Micro/Nanoplastics in plantation agricultural products: behavior process, phytotoxicity under biotic and abiotic stresses, and controlling strategies.","authors":"Zhihao Lin, Donghui Xu, Yiming Zhao, Bin Sheng, Zhijian Wu, Xiaobin Wen, Jie Zhou, Ge Chen, Jun Lv, Jing Wang, Guangyang Liu","doi":"10.1186/s12951-025-03314-0","DOIUrl":"10.1186/s12951-025-03314-0","url":null,"abstract":"<p><p>With the extensive utilization of plastic products, microplastics/nanoplastics (MPs/NPs) contamination not only poses a global hazard to the environment, but also induces a new threat to the growth development and nutritional quality of plantation agricultural products. This study thoroughly examines the behavior of MPs/NPs, including their sources, entry routes into plants, phytotoxicity under various biotic and abiotic stresses (e.g., salinity, polycyclic aromatic hydrocarbons, heavy metals, antibiotics, plasticizers, nano oxide, naturally occurring organic macromolecular compounds, invasive plants, Botrytis cinerea mycorrhizal fungi.) and controlling strategies. MPs/NPs in agricultural systems mainly originate from mulch, sewage, compost fertilizer, municipal solid waste, pesticide packaging materials, etc. They enter plants through endocytosis, apoplast pathways, crack-entry modes, and leaf stomata, affecting phenotypic, metabolic, enzymatic, and genetic processes such as seed germination, growth metabolism, photosynthesis, oxidative stress and antioxidant defenses, fruit yield and nutrient quality, cytotoxicity and genotoxicity. MPs/NPs can also interact with other environmental stressors, resulting in synergistic, antagonistic, or neutral effects on phytotoxicity. To address these challenges, this review highlights strategies to mitigate MPs/NPs toxicity, including the development of novel green biodegradable plastics, plant extraction and immobilization, exogenous plant growth regulator interventions, porous nanomaterial modulation, biocatalysis and enzymatic degradation. Finally, the study identifies current limitations and future research directions in this critical field.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"231"},"PeriodicalIF":10.6,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11927206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Small extracellular vesicles: crucial mediators for prostate cancer.","authors":"Sijie Tang, Huiying Cheng, Xueyan Zang, Jiawei Tian, Zhongli Ling, Lingling Wang, Wenrong Xu, Jiajia Jiang","doi":"10.1186/s12951-025-03326-w","DOIUrl":"10.1186/s12951-025-03326-w","url":null,"abstract":"<p><p>Small extracellular vesicles (sEVs) play a critical role in the progression, diagnosis, and treatment of prostate cancer (PCa), particularly within the tumor microenvironment (TME). Acting as novel biomarkers and agents for targeted biological therapy, sEVs contribute significantly to improving patient survival. These vesicles transport a variety of biomolecules, including proteins, nucleic acids, and lipids, which are instrumental in remodeling the TME, facilitating intercellular communication, and influencing key processes such as tumor growth, metastasis, and therapy resistance. A thorough understanding of sEV heterogeneity, including their biogenesis, characteristics, and potential applications, is essential. Recent advances have illuminated the origins, formation processes, and molecular cargo of PCa-derived sEVs (PCa-sEVs), enhancing our understanding of their role in disease progression. Furthermore, sEVs show promise as diagnostic markers, with potential applications in early detection and prognostic assessment in PCa. Therapeutically, natural and engineered sEVs offer versatile applications, including drug delivery, gene therapy, and immunomodulation, underscoring their potential in PCa management. This review delves into the substantial potential of sEVs in clinical practices for PCa.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"230"},"PeriodicalIF":10.6,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11927207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intranasal delivery of engineered extracellular vesicles promotes neurofunctional recovery in traumatic brain injury.","authors":"Pengtao Li, Sishuai Sun, Xingyu Zhu, Xiaoyu Liu, Rui Yin, Yihao Chen, Jianbo Chang, Liguo Ye, Jingxi Gao, Xiaoyan Zhao, Houshi Xu, Yue Wang, Wei Zuo, Zhao Sun, Shihua Wang, Xiao Zhang, Junji Wei, Robert Chunhua Zhao, Qin Han","doi":"10.1186/s12951-025-03181-9","DOIUrl":"10.1186/s12951-025-03181-9","url":null,"abstract":"<p><p>Traumatic brain injury (TBI) is a leading cause of disability in adults, significantly affecting patients' quality of life. Extracellular vesicles (EVs) derived from human adipose-derived mesenchymal stem cells (hADSCs) have demonstrated therapeutic potential in TBI treatment. However, their limited targeting ability, short half-life, and low bioavailability present significant challenges for clinical application. In this study, we engineered extracellular vesicles (EEVs) by transfecting hADSCs with lentivirus and incorporating ultra-small paramagnetic nanoparticles (USPNs), resulting in EVs with enhanced miRNA expression and targeted delivery capabilities. These EEVs were administered intranasally to specifically target injury sites, effectively modulating the NF-κB signaling pathway to suppress neuroinflammation. In both in vitro and in vivo assessments, EEVs exhibited superior efficacy in promoting neurofunctional recovery and neurogenesis after brain injury compared to unmodified EVs. Furthermore, validation using human brain organoid models confirmed EEVs' remarkable ability to suppress neuroinflammation, offering a promising strategy for TBI treatment.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"229"},"PeriodicalIF":10.6,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11927228/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miRNA let-7f-5p-encapsulated labial gland MSC-derived EVs ameliorate experimental Sjögren's syndrome by suppressing Th17 cells via targeting RORC/IL-17A signaling axis.","authors":"Yufei Xie, Maosheng Chai, Yixiao Xing, Peiru Zhou, Pan Wei, Hong Hua","doi":"10.1186/s12951-025-03308-y","DOIUrl":"10.1186/s12951-025-03308-y","url":null,"abstract":"<p><p>Sjögren's syndrome (SS) is an autoimmune disease primarily affecting salivary glands, with xerostomia as a distinct clinical manifestation. This disease also poses a significantly increased risk of lymphoma, severely impacting patients' quality of life. The imbalance between Th17 and Treg cells plays a critical role in SS progression, driving severe immune dysregulation, chronic inflammation, and escalating tissue dysfunction. However, current clinical treatments for SS still remain limited, and it continues to be recognized as a refractory disease. Therefore, the development of novel and effective therapeutic strategies is a pressing demand in clinical research. In recent years, extracellular vesicle (EV) therapy has emerged as a promising approach for autoimmune disease treatment, showing encouraging outcomes in modulating immune balance and alleviating symptoms. EVs carry diverse cargo, among which microRNAs (miRNAs) are highly abundant and play critical roles. These small RNAs are essential for EV-mediated functions, particularly in regulating gene expression and modulating the immune microenvironment. Our research team first isolated labial gland mesenchymal stem cells (LGMSCs) and their derived EVs (LGMSC-EVs), which offer potential therapeutic advantages in SS due to their salivary gland origin. Then we screened and identified the highly enriched miRNA let-7f-5p as a key regulator through miRNA profiling analysis. To achieve better therapeutic outcomes, we transfected exogenous miRNA let-7f-5p into LGMSC-EVs to upregulate its expression, thereby constructing let-7f-5p-encapsulated LGMSC-EVs. These modified EVs were subsequently tested in an experimental SS mouse model to evaluate their therapeutic potential. The upregulation of miRNA let-7f-5p in LGMSC-EVs significantly enhanced their therapeutic effects, resulting in clinical improvements such as increased salivary flow and reduced lymphocytic infiltration. Mechanistically, let-7f-5p-encapsulated LGMSC-EVs suppressed Th17 cells by directly targeting the 3'-untranslated region (3'UTR) of RORC, inhibiting the RORC/IL-17A signaling axis, and reducing IL-17A production, thereby restoring Th17/Treg balance and promoting an anti-inflammatory profile. Collectively, this let-7f-5p-encapsulated LGMSC-EV therapy offers a promising target-driven approach for the treatment of SS, achieving improved clinical outcomes and immune rebalance after modification with miRNA let-7f-5p, which presents new potential for the clinical treatment of SS.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"228"},"PeriodicalIF":10.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11927278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selenium nanoparticles activate selenoproteins to mitigate septic lung injury through miR-20b-mediated RORγt/STAT3/Th17 axis inhibition and enhanced mitochondrial transfer in BMSCs.","authors":"Wan-Jie Gu, Feng-Zhi Zhao, Wei Huang, Ming-Gao Zhu, Hai-Yan Huang, Hai-Yan Yin, Tianfeng Chen","doi":"10.1186/s12951-025-03312-2","DOIUrl":"10.1186/s12951-025-03312-2","url":null,"abstract":"<p><p>Sepsis-induced acute lung injury (ALI) remains a critical clinical challenge with complex inflammatory pathogenesis. While bone marrow mesenchymal stem cells (BMSCs) demonstrate therapeutic potential through anti-inflammatory and cytoprotective effects, their age-related functional decline limits clinical utility. This study developed chitosan-functionalized selenium nanoparticles (SeNPs@CS, 100 nm) to rejuvenate BMSCs through miR-20b-mediated selenoprotein biosynthesis. Mechanistic investigations revealed that SeNPs@CS-treated BMSCs exhibited enhanced mitochondrial transfer capacity, delivering functional mitochondria to damaged alveolar epithelial cells (AECII) for cellular repair. Concurrently, miR-20b upregulation suppressed the RORγt/STAT3/Th17 axis, reducing pro-inflammatory Th17 cell differentiation in CD4⁺ T lymphocytes. The dual-target mechanism integrates immunomodulation via Th17 pathway inhibition with mitochondrial rejuvenation therapy, representing a paradigm-shifting approach for ALI management. These engineered BMSCs mitigated inflammatory markers in murine models, demonstrating superior efficacy to conventional BMSC therapies. Our findings establish SeNPs@CS-modified BMSCs as a novel therapeutic platform combining nanotechnology-enhanced stem cell engineering with precision immunometabolic regulation, providing new avenues for the treatment of sepsis-induced ALI.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"226"},"PeriodicalIF":10.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11924768/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZnO-Cu/Mn nanozyme for rescuing the intestinal homeostasis in Salmonella-induced colitis.","authors":"Aimin Wu, Chen Liang, WenShuang Chen, ChangFang Lu, JunZhou Chen, Bing Wu, Daiwen Chen, Li He, Xianxiang Wang","doi":"10.1186/s12951-025-03283-4","DOIUrl":"10.1186/s12951-025-03283-4","url":null,"abstract":"<p><p>Salmonella is one of the most common foodborne pathogens, which can cause severe enteritis and intestinal microbiota imbalance. However, there are limited strategies currently available for preventing or treating Salmonella-induced colitis. Herein, we developed the Cu/Mn-co-doped ZnO tandem nanozyme (ZnO-CM) with pH-responsive multienzyme-mimicking activities via doping engineering for the treatment of Salmonella-induced colitis. Benefiting from the co-doping of Cu and Mn, ZnO-CM nanospheres exhibit remarkable peroxidase-like activity in acidic condition and superoxide dismutase- and catalase-like activities in neutral environment. Animal experiments show that ZnO-CM can efficiently inhibit bacterial growth, alleviate inflammation, and restore the intestinal barrier, resulting in good antibacterial and anti-inflammatory effects on Salmonella-induced colitis. Mechanistically, ZnO-CM functions through inhibiting the continuous accumulation of ROS, increasing the levels of tight junction proteins occludin and claudin-1, and decreasing the expression of pro-inflammatory cytokines IL-1β and IL-6 in intestine. This work not only presents an effective paradigm for Salmonella-induced colitis therapy, but also provides new sights into the prevention and treatment of other bacterial enteritis.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"225"},"PeriodicalIF":10.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11924796/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reducing severity of inflammatory bowel disease through colonization of Lactiplantibacillus plantarum and its extracellular vesicles release.","authors":"Yuanyuan Wu, Xinyue Huang, Qianbei Li, Chaoqun Yang, Xixin Huang, Hualongyue Du, Bo Situ, Lei Zheng, Zihao Ou","doi":"10.1186/s12951-025-03280-7","DOIUrl":"10.1186/s12951-025-03280-7","url":null,"abstract":"<p><p>Inflammatory bowel disease (IBD) is characterized by compromised intestinal barrier function and a lack of effective treatments. Probiotics have shown promise in managing IBD due to their ability to modulate the gut microbiota, enhance intestinal barrier function, and exert anti-inflammatory effects. However, the specific mechanisms through which probiotics exert these therapeutic effects in IBD treatment remain poorly understood. Our research revealed a significant reduction of Lactiplantibacillus plantarum (L. plantarum) in the gut microbiota of IBD patients. L. plantarum is a well-known probiotic strain in the list of edible probiotics, recognized for its beneficial effects on gut health, including its ability to strengthen the intestinal barrier and reduce inflammation. We demonstrated that supplementation with L. plantarum could alleviate IBD symptoms in mice, primarily by inhibiting apoptosis in intestinal epithelial cells through L. plantarum's bacterial extracellular vesicles (L. plant-EVs). This protective effect is dependent on the efficient uptake of L. plant-EVs by intestinal cells. Intriguingly, watermelon enhances L. plantarum colonization and L. plant-EVs release, further promoting intestinal barrier repair. Our findings contribute to the understanding of L. plant-EVs in the probiotic-based therapeutic approach for IBD, as they are promising candidates for nanoparticle-based therapeutic methods that are enhanced by natural diets such as watermelon. This study thereby offers a potential breakthrough in the management and treatment of IBD.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"227"},"PeriodicalIF":10.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11924789/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}