Journal of Nanobiotechnology最新文献

{"title":"Inflammation-targeted nanomedicine prevents tumor metastasis following photodynamic therapy by reversing epithelial-mesenchymal transition and ROS-mediated immunosuppression.","authors":"Zhengwei Song, Quanwei Sun, Wenshuo Yang, Yunlong Li, Chaoyu Hu, Chen Chen, Kang Liu, Wei Shen, Ye Yang, Dengke Yin","doi":"10.1186/s12951-025-03332-y","DOIUrl":"10.1186/s12951-025-03332-y","url":null,"abstract":"<p><strong>Background: </strong>Prolonging the duration of photodynamic therapy (PDT) enhances the level of reactive oxygen species (ROS), thereby facilitating tumor ablation. However, our findings indicated that excessive ROS not only induces epithelial-mesenchymal transition (EMT) but also creates an immunosuppressive microenvironment in tumor, thereby triggering tumor metastasis.</p><p><strong>Methods: </strong>We initially developed neutrophil membrane hybrid liposomes (NLs) that can specifically target inflamed tumor tissues following PDT. Then, we utilized NLs to encapsulate the antioxidant nanozyme FeGA and the antiplatelet drug Lysine Acetylsalicylate (LAS), resulting in the formulation NLASF.</p><p><strong>Results: </strong>Experimental results demonstrated that FeGA effectively scavenges ROS, thereby reversing the immunosuppressive microenvironment induced by prolonged PDT. Furthermore, the incorporation of LAS effectively disrupts the interaction between tumor cells and platelets, mitigating tumor EMT and inhibiting hematogenous tumor metastasis. In a breast cancer mouse model, we observed that treatment with NLASF led to a near-complete suppression of tumor lung metastasis following the prolonged PDT. Additionally, the in vivo application of NLASF did not result in any blood toxicity or organ toxicity, highlighting its significant advantages over the free drugs group.</p><p><strong>Conclusions: </strong>This study provides a novel approach to enhance the efficacy of PDT and successfully suppress PDT-mediated tumor metastasis.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"271"},"PeriodicalIF":10.6,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11969706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A smartphone-colorimetric dual-mode diagnosis method for breast cancer detection of BRCA-1 gene base on silver plated substrate and catalytic hairpin assembly amplification.","authors":"Zan Yu, Wenxin Liu, Lei Wang","doi":"10.1186/s12951-025-03340-y","DOIUrl":"10.1186/s12951-025-03340-y","url":null,"abstract":"<p><p>Breast cancer is the most prevalent malignancy among women worldwide. With rapid societal development, there has been a consistent increase in breast cancer incidence rates annually, accompanied by a progressively younger age of onset. Consequently, there is an urgent need to develop a rapid, sensitive, and highly specific approach for the identification of BRCA-1. In this study, we successfully devised a heterogeneous system based on G-quadruplex DNAzyme for label-free and enzyme-free visual detection of BRCA-1. Moreover, the distinctive G-quadruplex (G4) DNAzyme ensures precise and accurate detection through enzyme-free amplification, colorimetric detection, and catalytic hairpin assembly mechanisms. The utilization of this heterogeneous system effectively minimizes background interference while exhibiting exceptional sensitivity and accuracy with an impressive detection limit as low as 0.6 pM. Furthermore, real sample analysis yielded an area under the ROC curve value of 0.9825 which demonstrates its robust performance for early screening purposes when integrated with smartphone-assisted analysis, thus showcasing its potential for widespread application in early breast cancer diagnosis.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"266"},"PeriodicalIF":10.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11963687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Conditioning period impacts the morphology and proliferative effect of extracellular vesicles derived from rat adipose tissue derived stromal cell.","authors":"Anton Borger, Maximilian Haertinger, Flavia Millesi, Lorenz Semmler, Paul Supper, Sarah Stadlmayr, Anda Rad, Christine Radtke","doi":"10.1186/s12951-025-03345-7","DOIUrl":"10.1186/s12951-025-03345-7","url":null,"abstract":"","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"265"},"PeriodicalIF":10.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11963397/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosome-like vesicles encapsulated with specific microRNAs accelerate burn wound healing and ameliorate scarring.","authors":"Zhiyong Lei, Xiaojuan Chen, Kezhuo Chen, Pan Liu, Mingzhang Ao, Lu Gan, Longjiang Yu","doi":"10.1186/s12951-025-03337-7","DOIUrl":"10.1186/s12951-025-03337-7","url":null,"abstract":"<p><p>Burn injuries are prevalent, yet effective treatments remain elusive. Exosomes derived from mesenchymal stem cells (MSC-Ex) possess remarkable pro-regenerative properties for wound healing. Despite their potential, the challenge of mass production limits their clinical application. To address this, preparing exosome-like vesicles has become an international trend. In this study, 28 key microRNAs (miRNAs) with significant pro-proliferation, anti-inflammation, and anti-fibrosis functions were screened from MSC-Ex. These miRNAs were encapsulated into liposomes and then hybridized with extracellular vesicles derived from watermelon to create synthetic exosome-like vesicles. The fabricated vesicles exhibited similar particle size and zeta potential to MSC-Ex, demonstrating high serum stability and effectively resisting the degradation of miRNA by RNase. They were efficiently internalized by cells and enabled a high rate of lysosomal escape for miRNAs post cellular uptake, thereby effectively exerting their pro-proliferative, anti-inflammatory, and anti-fibrotic functions. Further experiments demonstrated that these vesicles efficiently accelerated burn wound healing and reduced scarring, with effects comparable to those of natural MSC-Ex. Based on these findings, the exosome-like vesicles fabricated in this study present a promising alternative to MSC-Ex in burn wound treatment.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"264"},"PeriodicalIF":10.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11963272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular particles: emerging insights into central nervous system diseases.","authors":"Shenyuan Chen, Qinghua Bao, Wenrong Xu, Xiao Zhai","doi":"10.1186/s12951-025-03354-6","DOIUrl":"10.1186/s12951-025-03354-6","url":null,"abstract":"<p><p>Extracellular particles (EPs), including extracellular vesicles (EVs) and non-vesicular extracellular particles (NVEPs), are multimolecular biomaterials released by cells that play a crucial role in intercellular communication. Recently, new subtypes of EPs associated with central nervous system (CNS), such as exophers and supermeres have been identified. These EPs provide new perspectives for understanding the pathological progression of CNS disorders and confer potential diagnostic value for liquid biopsies in neurodegenerative diseases (NDs). Moreover, EPs have emerged as promising drug delivery vehicles and targeted platforms for CNS-specific therapies. In this review, we delineate the landscape of EP subtypes and their roles in the pathophysiology of CNS diseases. We also review the recent advances of EP-based diagnosis in NDs and highlight the importance of analytical platforms with single-particle resolution in the exploitation of potential biomarkers. Furthermore, we summarize the application of engineered EVs in the treatment of CNS diseases and outline the underexplored potential of NVEPs as novel therapeutic agents.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"263"},"PeriodicalIF":10.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11960037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of apoptotic vesicles in modulating inflammation, immune responses, and tissue regeneration.","authors":"Mohammad Amin Khalilzad, Javad Mohammadi, Soumayeh Amirsaadat, Sajad Najafi, Sona Zare, Mohammad Ali Nilforoushzadeh, Mitra Khalilzad, Mohammad Amir Amirkhani, Aysan Peyrovan, Seyedeh Fatemeh Sadati Khalili, Atefeh Farahani, Solmaz Zare","doi":"10.1186/s12951-025-03278-1","DOIUrl":"10.1186/s12951-025-03278-1","url":null,"abstract":"<p><p>The process of apoptosis plays a crucial role in tissue homeostasis, immune system regulation, and organ formation. Apoptotic vesicles (ApoEVs) are involved in efferocytosis, the process by which phagocytes ingest dead cells. ApoEVs also have potential therapeutic applications in cancer treatment, ischemic diseases, and their anti-inflammatory properties make them incredibly versatile for medical applications. These vesicles can induce apoptosis in cancer cells, provide tumor antigens for cancer vaccines, and even serve as effective drug delivery systems. Moreover, they can target hypoxic cells, inhibit inflammatory cell death pathways, and promote tissue regeneration. Also, their potential in addressing inflammatory disorders such as gastrointestinal ailments, osteoarthritis, and diabetes is promising. Additionally, ApoEVs can polarize anti-inflammatory immune cells and suppress inflammatory immune responses which make them a viable option for addressing the unmet need for novel anti-inflammatory medications. Despite a wealth of reviews examining the applications of ApoEVs, very few have thoroughly investigated the mechanisms underlying their anti-inflammatory effects. This distinctive approach positions the current review as timely and immensely relevant, illuminating the intriguing ways these entities function beyond their established advantages.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"260"},"PeriodicalIF":10.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11960034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant volatiles-loaded core-shell micro-nano fibers to achieve efficient and sustained bisexual attraction to pests.","authors":"Chenglong Cui, Wenjie Shangguan, Kebin Li, Xingfu Jiang, Zhimin Wang, Jiao Yin, Lidong Cao","doi":"10.1186/s12951-025-03269-2","DOIUrl":"10.1186/s12951-025-03269-2","url":null,"abstract":"<p><strong>Background: </strong>Chemical pesticides face significant challenges regarding their efficacy and environmental impact. Plant-based food attractants have emerged as a promising green alternative for pest control. However, their field application is limited by the short duration of effectiveness, necessitating improved carrier systems for sustained release. Electrospinning is a promising technology in this field, with core-shell fibers offering superior performance in efficient loading and sustained release compared to uniaxial fibers, highlighting their potential for further development.</p><p><strong>Results: </strong>In this study, core-shell micro-nano fiber mats were prepared via coaxial electrospinning using multiple environmentally friendly polymers. These mats were firstly and successfully loaded with food attractants bisexually attractive to Loxostege sticticalis adults, including 1-octen-3-ol, trans-2-hexenal, linalool, and anethole, enabling sustained release and effective trapping. The components in the core-shell spinning solution were chemically compatible, and after spinning, the poly(3-hydroxybutyrate-co-4-hydroxybutyrate)/polycaprolactone (PHB/PCL) in the shell layer and polyethylene oxide (PEO) in the core layer formed core-shell fibers with clear boundaries. The mats achieved an average encapsulation efficiency of 78% for active ingredients, with a sustained release profile that delivered over 60% of the attractants within 80 days while mitigating early burst release. Electroantennogram and behavioral studies revealed that the mats retained electrophysiological activity for at least 90 days, effectively attracting male and female adult insects even after 75 days. Field trials demonstrated that the mats significantly outperformed commercial slow-release carriers, attracting a higher number of L. sticticalis adults. Additionally, the mats exhibited strong stress resistance, biodegradability, and environmental compatibility, effectively protecting active molecules while minimizing ecological impact.</p><p><strong>Conclusions: </strong>The developed fiber mats provide a highly efficient, eco-friendly carrier for plant-based food attractants, offering prolonged efficacy and improved insect trapping performance. This study highlights their potential for sustainable agriculture and pest management, paving the way for greener alternatives to chemical pesticides.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"259"},"PeriodicalIF":10.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11959770/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous nano-silicon enhances the ability of intercropped faba bean to alleviate cadmium toxicity and resist Fusarium wilt.","authors":"Chaosheng Luo, Ting Li, You Huang, Taiqin Liu, Yan Dong","doi":"10.1186/s12951-025-03330-0","DOIUrl":"10.1186/s12951-025-03330-0","url":null,"abstract":"<p><p>Excessive soil cadmium (Cd) and the accumulation of pathogens pose serious threats to legume growth. However, it remains unclear whether intercropping (IFcd) and its combined treatment with silicon nanoparticles (Si-NPs) (IFcd + Si) can alleviate these challenges under Cd stress, as well as the underlying mechanisms involved. This study systematically elucidated the mechanism of faba bean-wheat intercropping and Si-NPs regulating faba bean growth under Cd stress using rhizosphere metabolomics and 16 S rRNA microbiome analysis. The results showed that IFcd and IFcd + Si treatments significantly reduced Cd accumulation by 17.3% and 56.2%, and Fusarium wilt incidence by 11.1% and 33.3%, respectively, compared with monoculture faba bean (MFcd) while promoting root and plant growth. These treatments reduced oxidative stress markers, including H₂O₂, MDA, and O₂^-, and increased the activity of defense enzymes, such as SOD, APX, and POD in plants. Furthermore, they increased NH₄⁺-N and available potassium levels in rhizosphere soils. Interestingly, the NH₄⁺-N content increased and was significantly positively correlated with urease (URE) activity and negatively correlated with Cd. Beneficial bacteria and functional metabolites were enriched in the rhizosphere of faba bean. Joint analysis revealed increased relative abundances of Sphingomonas, Intrasporangium, and Streptomyces, which were positively correlated with antibacterial metabolites, such as sordarin, lactucin, and 15-methylpalmate. This explains the reduced Cd accumulation and Fusarium wilt in plants. These findings provide mechanistic insights into how intercropping with Si-NPs mitigates Cd stress and controls soil-borne diseases by regulating rhizosphere metabolites, bacterial communities, and plant resistance.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"262"},"PeriodicalIF":10.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11959883/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral administration of Momordica charantia-derived extracellular vesicles alleviates ulcerative colitis through comprehensive renovation of the intestinal microenvironment.","authors":"Bowen Gao, Xiaoling Huang, Junlong Fu, Liyuan Chen, Zhichao Deng, Shuhui Wang, Yuanyuan Zhu, Chenxi Xu, Yujie Zhang, Mingxin Zhang, Lina Chen, Manli Cui, Mingzhen Zhang","doi":"10.1186/s12951-025-03346-6","DOIUrl":"10.1186/s12951-025-03346-6","url":null,"abstract":"<p><strong>Background: </strong>Ulcerative colitis (UC) is an inflammatory bowel disease (IBD), accompanied by intense inflammation, oxidative stress, and intestinal microbiota dysbiosis. Current treatments using chemotherapeutic drugs or immunosuppressants have limited effectiveness and side effects. Therefore, the development of safe, effective, and multi-targeting therapies for IBD is of great importance. Momordica charantia exhibits antioxidant, anti-inflammatory, and intestinal microbiota-regulating properties, suggesting that Momordica charantia-derived extracellular vesicles (MCEVs) have the potential for UC management.</p><p><strong>Results: </strong>We extracted MCEVs using differential centrifugation and density gradient centrifugation. The results showed that MCEVs possessed high purity, even particle size, and excellent stability. In vitro, MCEVs were shown to inhibit macrophage inflammatory responses, scavenge reactive oxygen species (ROS), and protect cells from oxidative damage. Transcriptomics analysis revealed that MCEVs may alleviate mitochondria-dependent apoptosis by safeguarding the integrity of the mitochondrial structure and regulating the expression of apoptosis-related proteins. Furthermore, all components of MCEVs contributed to their pharmacological activity. In vivo, MCEVs had better retention in the inflamed colon and significantly alleviated UC through a comprehensive renovation of the intestinal microenvironment.</p><p><strong>Conclusion: </strong>These findings suggested that MCEVs own considerable potential as natural nanotherapeutics for UC treatment.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"261"},"PeriodicalIF":10.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11959773/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Addressing the challenges of infectious bone defects: a review of recent advances in bifunctional biomaterials.","authors":"Huaiyuan Zhang, Wenyu Qiao, Yu Liu, Xizhou Yao, Yonghua Zhai, Longhai Du","doi":"10.1186/s12951-025-03295-0","DOIUrl":"10.1186/s12951-025-03295-0","url":null,"abstract":"<p><p>Infectious bone defects present a substantial clinical challenge due to the complex interplay between infection control and bone regeneration. These defects often result from trauma, autoimmune diseases, infections, or tumors, requiring a nuanced approach that simultaneously addresses infection and promotes tissue repair. Recent advances in tissue engineering and materials science, particularly in nanomaterials and nano-drug formulations, have led to the development of bifunctional biomaterials with combined osteogenic and antibacterial properties. These materials offer an alternative to traditional bone grafts, minimizing complications such as multiple surgeries, high antibiotic dosages, and lengthy recovery periods. This review examines the repair mechanisms in the infectious microenvironment and highlights various bifunctional biomaterials that foster both anti-infective and osteogenic processes. Emerging design strategies are also discussed to provide a forward-looking perspective on treating infectious bone defects with clinically significant outcomes.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"257"},"PeriodicalIF":10.6,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954225/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}