Journal of Nanobiotechnology最新文献_第10页

From ROS scavenging to boosted osseointegration: cerium-containing mesoporous bioactive glass nanoparticles functionalized implants in diabetes. 从清除 ROS 到促进骨结合：含铈介孔生物活性玻璃纳米粒子功能化植入物在糖尿病中的应用。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2024-10-18 DOI: 10.1186/s12951-024-02865-y

Xue Jiang, Jianxu Wei, Xinxin Ding, Kai Zheng, Tian Zhou, Junyu Shi, Hongchang Lai, Shujiao Qian, Xiaomeng Zhang

{"title":"From ROS scavenging to boosted osseointegration: cerium-containing mesoporous bioactive glass nanoparticles functionalized implants in diabetes.","authors":"Xue Jiang, Jianxu Wei, Xinxin Ding, Kai Zheng, Tian Zhou, Junyu Shi, Hongchang Lai, Shujiao Qian, Xiaomeng Zhang","doi":"10.1186/s12951-024-02865-y","DOIUrl":"https://doi.org/10.1186/s12951-024-02865-y","url":null,"abstract":"Excessive production of reactive oxygen species (ROS) around titanium implants under diabetic conditions causes persistent inflammation, leading to poor osseointegration and even implant failure. Surface modification is an effective way to promote ROS clearance, alleviate inflammation, and stimulate bone formation. In this study, a multifunctional coating is fabricated by introducing cerium (Ce)-containing mesoporous bioactive glass nanoparticles (Ce-MBGNs) onto the titanium surface via an electrophoretic deposition method. The incorporation of Ce-MBGNs remarkably improves surface hydrophilicity by increasing the surface areas. The bioactive ions are appropriately released, thereby promoting mesenchymal stem cell proliferation and differentiation under diabetic conditions. The conversion between Ce(III) and Ce(IV) endows Ce-MBGNs coating with antioxidative nanoenzymes properties to scavenge diabetes-induced ROS, resulting in macrophage polarization towards the anti-inflammatory phenotype. The therapeutic effect of Ce-MBGNs-modified titanium implants is also verified in diabetic rats by inhibiting inflammatory responses and accelerating early osseointegration. Taken together, the findings reveal that the ROS-scavenging and immunomodulation activity of the Ce-MBGNs coating contributes to enhanced osseointegration, and provides a novel implant surface for diabetic patients.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"22 1","pages":"639"},"PeriodicalIF":10.6,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11488221/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selenium nanoparticles ameliorate lumbar disc degeneration by restoring GPX1-mediated redox homeostasis and mitochondrial function of nucleus pulposus cells. 硒纳米粒子通过恢复 GPX1 介导的氧化还原平衡和髓核细胞线粒体功能改善腰椎间盘退变。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2024-10-18 DOI: 10.1186/s12951-024-02890-x

Wei He, Xin Tian, Quan Zhou, Jiaheng Lv, Yangfeng Li, Chenyang Jin, Hao Liu, Huiling Yang, Yong Xu, Fan He, Tao Liu

{"title":"Selenium nanoparticles ameliorate lumbar disc degeneration by restoring GPX1-mediated redox homeostasis and mitochondrial function of nucleus pulposus cells.","authors":"Wei He, Xin Tian, Quan Zhou, Jiaheng Lv, Yangfeng Li, Chenyang Jin, Hao Liu, Huiling Yang, Yong Xu, Fan He, Tao Liu","doi":"10.1186/s12951-024-02890-x","DOIUrl":"https://doi.org/10.1186/s12951-024-02890-x","url":null,"abstract":"Intervertebral disc degeneration (IVDD) is a prevalent musculoskeletal disorder that involves the excessive accumulation of reactive oxygen species (ROS), resulting in mitochondrial dysfunction and matrix metabolism imbalance in nucleus pulposus cells (NPCs). Selenium, an indispensable trace element, plays a crucial role in maintaining mitochondrial redox homeostasis by being incorporated into antioxidant selenoproteins as selenocysteine. In this study, we employed a straightforward synthesis method to produce selenium nanoparticles (SeNPs) with consistent size and distribution, and evaluated their potential protective effects in ameliorating IVDD. In a simulated inflammatory environment induced by interleukin-1beta (IL-1β) in vitro, SeNPs demonstrated a protective effect on the matrix synthesis capacity of NPCs through the up-regulation of aggrecan and type II collagen, while concurrently suppressing the expression of matrix degradation enzymes including MMP13 and ADAMTS5. Additionally, SeNPs preserved mitochondrial integrity and restored impaired mitochondrial energy metabolism by activating glutathione peroxidase1 (GPX1) to rebalance redox homeostasis. In a rat lumbar disc model induced by puncture, the local administration of SeNPs preserved the hydration of nucleus pulposus tissue, promoted matrix deposition, and effectively mitigated the progression of IVDD. Our results indicate that the enhancement of GPX1 by SeNPs may offer a promising therapeutic approach for IVDD by restoring mitochondrial function and redox homeostasis.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"22 1","pages":"634"},"PeriodicalIF":10.6,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11487783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibody-targeted T cells and natural killer cells for cancer immunotherapy. 用于癌症免疫疗法的抗体靶向 T 细胞和自然杀伤细胞。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2024-10-18 DOI: 10.1186/s12951-024-02898-3

Ashley R Sutherland, Brijesh Parlekar, David W Livingstone, Andrés X Medina, Wendy Bernhard, Tays Hernández García, John DeCoteau, C Ronald Geyer

{"title":"Antibody-targeted T cells and natural killer cells for cancer immunotherapy.","authors":"Ashley R Sutherland, Brijesh Parlekar, David W Livingstone, Andrés X Medina, Wendy Bernhard, Tays Hernández García, John DeCoteau, C Ronald Geyer","doi":"10.1186/s12951-024-02898-3","DOIUrl":"https://doi.org/10.1186/s12951-024-02898-3","url":null,"abstract":"Background: Adoptive cell cancer therapies aim to re-engineer a patient's immune cells to mount an anti-cancer response. Chimeric antigen receptor T and natural killer cells have been engineered and proved successful in treating some cancers; however, the genetic methods for engineering are laborious, expensive, and inefficient and can cause severe toxicities when they over-proliferate.Results: We examined whether the cell-killing capacity of activated T and NK cells could be targeted to cancer cells by anchoring antibodies to their cell surface. Using metabolic glycoengineering to introduce azide moieties to the cellular surface, we covalently attached a dibenzocyclooctyne-modified antibody using the strain-promoted alkyne azide cycloaddition reaction, creating antibody-conjugated T and NK cells. We targeted the immune cells to tumors possessing the xenoantigen, N-glycolyl neuraminic acid GM3 ganglioside, using the 14F7hT antibody. These activated T and NK cells are \"armed\" with tumour-homing capabilities that specifically lyses antigen-positive cancer cells without off-target toxicities. Moreover, when exposed to target cells, 14F7hT-conjugated T cells that are not preactivated exhibit increased perforin, granzyme, CD69, and CD25 expression and specific cell killing.Conclusions: This research shows the potential for a non-genetic method for redirecting cytotoxic immune cells as a feasible and effective approach for tumor-targeted cell immunotherapy.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"22 1","pages":"640"},"PeriodicalIF":10.6,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11488284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel combination therapy using recombinant oncolytic adenovirus silk hydrogel and PD-L1 inhibitor for bladder cancer treatment. 利用重组溶瘤腺病毒丝水凝胶和 PD-L1 抑制剂治疗膀胱癌的新型联合疗法。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2024-10-18 DOI: 10.1186/s12951-024-02903-9

Wenqiang Zhang, Jianqiang Zhang, Jingwei Zhang, Jing Chu, Zhenxing Zhang

{"title":"Novel combination therapy using recombinant oncolytic adenovirus silk hydrogel and PD-L1 inhibitor for bladder cancer treatment.","authors":"Wenqiang Zhang, Jianqiang Zhang, Jingwei Zhang, Jing Chu, Zhenxing Zhang","doi":"10.1186/s12951-024-02903-9","DOIUrl":"https://doi.org/10.1186/s12951-024-02903-9","url":null,"abstract":"Recombinant oncolytic adenovirus offers a novel and promising cancer treatment approach, but its standalone efficacy remains limited. This study investigates a combination treatment strategy by co-administering recombinant oncolytic Adv-loaded silk hydrogel with a PD-L1 inhibitor for patients with bladder cancer to enhance treatment outcomes. Bladder cancer tissues from mice were collected and subjected to single-cell sequencing, identifying CRB3 as a key gene in malignant cells. Differential expression and functional enrichment analyses were performed, validating CRB3's inhibitory role through in vitro experiments showing suppression of bladder cancer cell proliferation, migration, and invasion. Recombinant oncolytic adenoviruses encoding CRB3 and GM-CSF were constructed and encapsulated in silk hydrogel to enhance drug loading and release efficiency. In vivo experiments demonstrated that the nano-composite hydrogel significantly inhibited tumor growth and increased immune infiltration in tumor tissues. Co-administration of adenovirus silk hydrogel (Adv-CRB3@gel) with a PD-L1 inhibitor significantly enhanced T-cell infiltration and tumor killing. The combination of recombinant oncolytic Adv-loaded nano-composite hydrogel encoding CRB3 and GM-CSF with a PD-L1 inhibitor improves bladder cancer treatment outcomes by effectively recruiting T cells, providing a novel therapeutic strategy.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"22 1","pages":"638"},"PeriodicalIF":10.6,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11487847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly integrated, self-powered and activatable bipedal DNA nanowalker for imaging of base excision repair in living cells. 用于活细胞碱基切除修复成像的高度集成、自供电和可激活的双足 DNA 纳米步行者。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2024-10-18 DOI: 10.1186/s12951-024-02927-1

Rongji Lai, Xianghe Pan, Yingfeng Qin, Jialin Liang, Liu Wu, Meiyu Dong, Jia Chen, Jin-Wen Liu

{"title":"Highly integrated, self-powered and activatable bipedal DNA nanowalker for imaging of base excision repair in living cells.","authors":"Rongji Lai, Xianghe Pan, Yingfeng Qin, Jialin Liang, Liu Wu, Meiyu Dong, Jia Chen, Jin-Wen Liu","doi":"10.1186/s12951-024-02927-1","DOIUrl":"https://doi.org/10.1186/s12951-024-02927-1","url":null,"abstract":"DNA walkers have attracted considerable attention in biosensing and bioimaging. Compared with the conventional single leg-based DNA walker, the bipedal DNA walker has remarkable advantages, with improved sensitivity and fast kinetics, and can work efficiently in a crowded cellular environment. However, most reported bipedal DNA walkers are powered by exogenous supplementation, and elaborate DNA sequence designs, auxiliary additives or extra carriers are often needed. A highly integrated bipedal DNA walker that can address robustness, sensitivity and consistency issues in a single system is highly desirable but remains a great challenge. We herein report a novel bipedal DNA nanowalker system through simple assembly of a DNA substrate, hairpin functionalized-AuNPs (AuNPs-H2), and a blocked Mn2+-dependent DNAzyme hairpin (H1) on degradable MnO2 nanosheets, which holds great potential for living cell operation. Highly integrated features enable the simultaneous delivery of core components of the bipedal DNA walker, including a walking track (AuNPs-H2), a walking strand (H1 cleaved by APE1), and a driving force (Mn2+-dependent DNAzyme cleavage) as a whole, thereby enhancing the control of the spatiotemporal distribution of these components at the intracellular target sites. The redox reaction between the MnO2 nanosheets and GSH inside the cells not only consumed the intracellular GSH to improve the biostability of the walking track but also generated abundant Mn2+ as a cofactor of the DNAzyme. As a proof of concept, the developed nanowalker was demonstrated to work efficiently for monitoring base excision repair (BER)-related human apurinic/apyrimidinic endonuclease 1 (APE1) in living cells, highlighting the great potential of the bipedal DNA nanowalker in biological systems.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"22 1","pages":"636"},"PeriodicalIF":10.6,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11487878/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Layer by layer self-assembled hyaluronic acid nanoarmor for the treatment of ulcerative colitis. 用于治疗溃疡性结肠炎的逐层自组装透明质酸纳米armor。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2024-10-18 DOI: 10.1186/s12951-024-02933-3

Xinxin Zhao, Yuchen Zhang, Pengchong Wang, Kailai Liu, Yunhe Zheng, Jinpeng Wen, Ke Wang, Xiaopeng Wen

{"title":"Layer by layer self-assembled hyaluronic acid nanoarmor for the treatment of ulcerative colitis.","authors":"Xinxin Zhao, Yuchen Zhang, Pengchong Wang, Kailai Liu, Yunhe Zheng, Jinpeng Wen, Ke Wang, Xiaopeng Wen","doi":"10.1186/s12951-024-02933-3","DOIUrl":"https://doi.org/10.1186/s12951-024-02933-3","url":null,"abstract":"Natural compound-based treatments provide innovative ways for ulcerative colitis therapy. However, poor targeting and rapid degradation curtail its application, which needs to be addressed. Inspired by biomacromolecule-based materials, we have developed an orally administrated nanoparticle (GBP@HA NPs) using bovine serum albumin as a carrier for polyphenol delivery. The system synergizes galactosylated bovine serum albumin with two polyphenols, epigallocatechin gallate and tannic acid, which is then encased in \"nanoarmor\" of ε-Polylysine and hyaluronic acid to boost its stability and targeting. Remarkably, the nanoarmor demonstrated profound therapeutic effects in both acute and chronic mouse models of ulcerative colitis, mitigating disease symptoms via multiple mechanisms, regulating inflammation related factors and exerting a modulatory impact on gut microbiota. Further mechanistic investigations indicate that GBP@HA NPs may act through several pathways, including modulation of Keap1-Nrf2 and NF-κB signaling, as well as Caspase-1-dependent pyroptosis. Consequently, this novel armored nanotherapy promotes the way for enhanced polyphenol utilization in ulcerative colitis treatment research.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"22 1","pages":"633"},"PeriodicalIF":10.6,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11488142/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stable triangle: nanomedicine-based synergistic application of phototherapy and immunotherapy for tumor treatment. 稳定三角：基于纳米药物的光疗法和免疫疗法在肿瘤治疗中的协同应用。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2024-10-18 DOI: 10.1186/s12951-024-02925-3

Wenjing Cai, Tuyue Sun, Chenyu Qiu, Huixiang Sheng, Ruijie Chen, Congying Xie, Longfa Kou, Qing Yao

{"title":"Stable triangle: nanomedicine-based synergistic application of phototherapy and immunotherapy for tumor treatment.","authors":"Wenjing Cai, Tuyue Sun, Chenyu Qiu, Huixiang Sheng, Ruijie Chen, Congying Xie, Longfa Kou, Qing Yao","doi":"10.1186/s12951-024-02925-3","DOIUrl":"https://doi.org/10.1186/s12951-024-02925-3","url":null,"abstract":"In recent decades, cancer has posed a challenging obstacle that humans strive to overcome. While phototherapy and immunotherapy are two emerging therapies compared to traditional methods, they each have their advantages and limitations. These limitations include easy metastasis and recurrence, low response rates, and strong side effects. To address these issues, researchers have increasingly focused on combining these two therapies by utilizing a nano-drug delivery system due to its superior targeting effect and high drug loading rate, yielding remarkable results. The combination therapy demonstrates enhanced response efficiency and effectiveness, leading to a preparation that is highly targeted, responsive, and with low recurrence rates. This paper reviews several main mechanisms of anti-tumor effects observed in combination therapy based on the nano-drug delivery system over the last five years. Furthermore, the challenges and future prospects of this combination therapy are also discussed.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"22 1","pages":"635"},"PeriodicalIF":10.6,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11488210/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking nature's brilliance: using Antarctic extremophile Shewanella baltica to biosynthesize lanthanide-containing nanoparticles with optical up-conversion. 释放大自然的光彩：利用南极极端嗜石斑菌生物合成具有光学上转换功能的含镧纳米粒子。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2024-10-18 DOI: 10.1186/s12951-024-02874-x

Nia Oetiker, Juan José León, Mark Swihart, Kaiwen Chen, Blaine A Pfeifer, Avisek Dutta, Artem Pliss, Andrey N Kuzmin, José Manuel Pérez-Donoso, Paras N Prasad

{"title":"Unlocking nature's brilliance: using Antarctic extremophile Shewanella baltica to biosynthesize lanthanide-containing nanoparticles with optical up-conversion.","authors":"Nia Oetiker, Juan José León, Mark Swihart, Kaiwen Chen, Blaine A Pfeifer, Avisek Dutta, Artem Pliss, Andrey N Kuzmin, José Manuel Pérez-Donoso, Paras N Prasad","doi":"10.1186/s12951-024-02874-x","DOIUrl":"https://doi.org/10.1186/s12951-024-02874-x","url":null,"abstract":"Both lanthanide-containing and fluorine-containing nanomaterials present challenging targets for microbial biosynthesis because these elements are toxic to most bacteria. Here, we overcome these challenges by using an Antarctic Shewanella baltica strain that tolerates these elements and report the first biosynthesis of lanthanide-doped fluoride nanoparticles (NPs) from them. NaYF4 NPs doped with Er3+/Yb3+ are prototypical lanthanide-based upconverting nanoparticles (UCNPs) with upconverted luminescence at visible wavelengths under infrared excitation. However, their synthesis employs high precursor concentrations, organic solvents, and elevated temperatures. Microbial biosynthesis offers a greener alternative but has not been explored for these materials. Here, we harness an extremophile S. baltica strain to biosynthesize UCNPs at room temperature, based upon its high tolerance for fluoride and lanthanide ions and the observation that tolerance of lanthanides increased in the presence of fluoride. Our biosynthesis produces electron-dense nanostructures composed of Na, Y, F, Yb, and Er in the bacterial periplasm, adhered to the outer cell membrane, and dispersed extracellularly, which exhibited up-converted emission under 980 nm excitation. This suggests that extracellular or periplasmic mineralization of lanthanides as fluorides protects the bacteria from lanthanide toxicity. Subsequent heating both enhanced upconverted emission from UCNPs and allowed observation of their crystallinity in transmission electron microscopy (TEM). This work establishes the first biosynthesis of NaYF4:Yb: Er UCNPs, advancing both nanotechnology and biotechnology.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"22 1","pages":"637"},"PeriodicalIF":10.6,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11488251/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brain-targeted ursolic acid nanoparticles for anti-ferroptosis therapy in subarachnoid hemorrhage. 脑靶向熊果酸纳米粒子用于蛛网膜下腔出血的抗铁锈色素沉着治疗。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2024-10-18 DOI: 10.1186/s12951-024-02866-x

Yong Li, Xinyi Zhu, Wei Xiong, Qingyu Zhao, Youdong Zhou, Yujia Guo, Baohui Liu, Mingchang Li, Qianxue Chen, Xiaobing Jiang, Yangzhi Qi, Qingsong Ye, Gang Deng

{"title":"Brain-targeted ursolic acid nanoparticles for anti-ferroptosis therapy in subarachnoid hemorrhage.","authors":"Yong Li, Xinyi Zhu, Wei Xiong, Qingyu Zhao, Youdong Zhou, Yujia Guo, Baohui Liu, Mingchang Li, Qianxue Chen, Xiaobing Jiang, Yangzhi Qi, Qingsong Ye, Gang Deng","doi":"10.1186/s12951-024-02866-x","DOIUrl":"10.1186/s12951-024-02866-x","url":null,"abstract":"Background: Subarachnoid hemorrhage (SAH) is a life -threatening cerebrovascular disease, where early brain injury (EBI) stands as a primary contributor to mortality and unfavorable patient outcomes. Neuronal ferroptosis emerges as a key pathological mechanism underlying EBI in SAH. Targeting ferroptosis for therapeutic intervention in SAH holds significant promise as a treatment strategy.Methods: SAH model was induced via intravascular puncture and quantitatively assessed the presence of neuronal ferroptosis in the early phase of SAH using FJC staining, Prussian blue staining, as well as malondialdehyde (MDA) and glutathione (GSH) measurements. Hyaluronic acid-coated ursolic acid nanoparticles (HA-PEG-UA NPs) were prepared using the solvent evaporation method. We investigated the in vivo distribution of HA-PEG-UA NPs in SAH model through IVIS and fluorescence observation, and examined their impact on short-term neurological function and cortical neurological injury. Finally, we assessed the effect of UA on the Nrf-2/SLC7A11/GPX4 axis via Western Blot analysis.Results: We successfully developed self-assembled UA NPs with hyaluronic acid to target the increased CD44 expression in the SAH-afflicted brain. The resulting HA-PEG-UA NPs facilitated delivery and enrichment of UA within the SAH-affected region. The targeted delivery of UA to the SAH region can effectively inhibit neuronal ferroptosis, improve neurological deficits, and prognosis in mice. Its mechanism of action is associated with the activation of the Nrf-2/SLC7A11/GPX4 signaling pathway.Conclusions: Brain-targeted HA-PEG-UA NPs was successfully developed and hold the potential to enhance SAH prognosis by limiting neuronal ferroptosis via modulation of the Nrf-2/SLC7A11/GPX4 signal.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"22 1","pages":"641"},"PeriodicalIF":10.6,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11490124/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recombinant Lactococcus lactis secreting FliC protein nanobodies for resistance against Salmonella enteritidis invasion in the intestinal tract. 重组乳球菌分泌 FliC 蛋白纳米抗体，抵御肠道沙门氏菌入侵。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2024-10-16 DOI: 10.1186/s12951-024-02904-8

Ming Yang, Kui Gu, Qiang Xu, Renqiao Wen, Jinpeng Li, Changyu Zhou, Yu Zhao, Miwan Shi, Yuan Weng, Boyan Guo, Changwei Lei, Yong Sun, Hongning Wang

{"title":"Recombinant Lactococcus lactis secreting FliC protein nanobodies for resistance against Salmonella enteritidis invasion in the intestinal tract.","authors":"Ming Yang, Kui Gu, Qiang Xu, Renqiao Wen, Jinpeng Li, Changyu Zhou, Yu Zhao, Miwan Shi, Yuan Weng, Boyan Guo, Changwei Lei, Yong Sun, Hongning Wang","doi":"10.1186/s12951-024-02904-8","DOIUrl":"https://doi.org/10.1186/s12951-024-02904-8","url":null,"abstract":"Salmonella Enteritidis is a major foodborne pathogen throughout the world and the increase in antibiotic resistance of Salmonella poses a significant threat to public safety. Natural nanobodies exhibit high affinity, thermal stability, ease of production, and notably higher diversity, making them widely applicable for the treatment of viral and bacterial infections. Recombinant expression using Lactococcus lactis leverages both acid resistance and mucosal colonization properties of these bacteria, allowing the effective expression of exogenous proteins for therapeutic effects. In this study, nine specific nanobodies against the flagellar protein FliC were identified and expressed. In vitro experiments demonstrated that FliC-Nb-76 effectively inhibited the motility of S. Enteritidis and inhibited its adhesion to and invasion of HIEC-6, RAW264.7, and chicken intestinal epithelial cells. Additionally, a recombinant L. lactis strain secreting the nanobody, L. lactis-Nb76, was obtained. Animal experiments confirmed that it could significantly reduce the mortality rates of chickens infected with S. Enteritidis, together with alleviating the inflammatory response caused by the pathogen. These results provide a novel strategy for the treatment of antibiotic-resistant S. Enteritidis infection in the intestinal tract.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"22 1","pages":"629"},"PeriodicalIF":10.6,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11481460/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0