Journal of Nanobiotechnology最新文献

{"title":"Nanomedicine in cardiovascular and cerebrovascular diseases: targeted nanozyme therapies and their clinical potential and current challenges.","authors":"Yanhua Jiang, Yongjian Zhou, Zhe Li, Liang Guo","doi":"10.1186/s12951-025-03590-w","DOIUrl":"10.1186/s12951-025-03590-w","url":null,"abstract":"","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"543"},"PeriodicalIF":12.6,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12302787/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoparticles induced cuproptosis to enhance antitumor immunotherapy.","authors":"Wen Deng, Zichen Zhong, Haojie Shang, Yonghua Tong, Qiu Huang, Yu He, Jian Wu, Xiaozhuo Ba, Zhiqiang Chen, Yuan Chen, Kun Tang","doi":"10.1186/s12951-025-03616-3","DOIUrl":"10.1186/s12951-025-03616-3","url":null,"abstract":"<p><p>Immunotherapy is a highly promising cancer treatment method. However, it is limited by low immunogenicity and an immunosuppressive microenvironment, which could be relieved by immunogenic cell death (ICD). Currently, effective ICD is primarily achieved through apoptosis induction, but tumor cells' resistance to apoptosis limits its antitumor efficacy. Therefore, developing new cell death modalities with high immunogenicity for cancer immunotherapy is of great significance. Cuproptosis, a newly discovered form of programmed cell death, can effectively circumvent tumor cells' resistance to apoptosis. Various Cu ionophores have been studied as anticancer drugs to promote cuproptosis, but the lack of tumor specificity remains one of the major challenges in this field. In contrast, nanoparticles tend to preferentially accumulate in tumor tissues due to the enhanced permeability and retention (EPR) effect, and they can be surface-modified to achieve active tumor targeting capabilities. Recently, many unique physicochemical properties of nanoparticles have been designed as nano-inducers of cuproptosis, successfully enhancing immunotherapy. Based on this, this review detailedly summarized various strategies and applications of nanoparticles-induced cuproptosis in tumor cells. The role of Cu metabolism and homeostasis in tumorigenesis and development, the molecular mechanisms of cuproptosis and different cuproptosis inducers with promising application prospects, as well as the interaction between cuproptosis and immunotherapy have also been reviewed. Finally, we presented the limitations and future prospects of cuproptosis nano-inducers, hoping to provide a new strategy to enhance antitumor immunotherapy.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"544"},"PeriodicalIF":12.6,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12302891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bandgap-engineered proteinic near-infrared nanodots for localized precision cancer theranostics.","authors":"Mou Seung Kim, In Ho Nam, Hyung Joon Cha, Yun Kee Jo","doi":"10.1186/s12951-025-03619-0","DOIUrl":"10.1186/s12951-025-03619-0","url":null,"abstract":"","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"541"},"PeriodicalIF":12.6,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12296602/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144718028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular vesicles from ovarian cancer cells induce senescent lipid-laden macrophages to facilitate omental metastasis.","authors":"Guoqing Li, Xiaoling Zhou, Wenhan Li, Qiulei Wu, Tong Liu, Lin Huang, Xiaoli Liu, Jing Zhao, Xiaohan Xu, Linjuan Xu, Zehua Wang, Lanqing Gong, Liqiong Cai, Jing Cai","doi":"10.1186/s12951-025-03612-7","DOIUrl":"10.1186/s12951-025-03612-7","url":null,"abstract":"<p><strong>Background: </strong>Ovarian cancer exhibits striking metastatic tropism for the omentum, where lipid-laden macrophages are key mediators that fuel disease progression. However, the mechanisms governing their formation and pro-metastatic functions remain poorly understood. As extracellular vesicles (EVs) have as critical regulators of tumor-stroma crosstalk in metastatic niches, we sought to define how ovarian cancer-derived EVs orchestrate macrophages and adipocytes, and their impact on omental metastasis, aiming to explore potential therapeutic interventions.</p><p><strong>Results: </strong>Single-cell transcriptomics of ovarian cancer revealed a distinct lipid-laden macrophage population in omentum, whose abundance correlated with metastatic burden and poor survival. Proteomics revealed that EVs from highly metastatic ovarian cancer cells were enriched in lipid metabolism regulators. In vivo experiments demonstrated that these tumor-derived vesicles mediated macrophage reprogramming, driving the acquisition of a pro-metastatic phenotype. Quantitative lipidomic profiling and lipid staining approaches confirmed the progressive lipid-laden in EV-treated macrophages. Using a patient-derived omentum-macrophage co-culture system, we demonstrated that tumor-derived EVs stimulate lipid release from omental adipocytes, which macrophages subsequently internalize through CD36-dependent uptake to drive lipid accumulation. This metabolic reprogramming culminated in cellular senescence, as evidenced by classical biomarkers including SA-β-galactosidase activity, elevated p16-INK4A and p53 levels, and the development of a matrix metalloproteinase-enriched senescence-associated secretory phenotype. Immunohistochemistry of clinical specimens demonstrated overexpression of CD36 correlated with omental metastasis and poor survival in ovarian cancer. In vivo experiments demonstrated that CD36 inhibition and senolytic therapy attenuated omental metastasis.</p><p><strong>Conclusions: </strong>This study unveils an EV-driven mechanism of adipose tropism in ovarian cancer metastasis, where EVs promote the formation of senescent lipid-laden macrophages via CD36-mediated lipid uptake, remodeling the metastatic niche. Targeting CD36 and senescent cells offers a promising therapeutic strategy against omental metastasis.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"540"},"PeriodicalIF":12.6,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12297738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144718029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced therapeutic scaffolds of biomimetic periosteum for functional bone regeneration.","authors":"Archita Gupta, Kyung Wook Kim, Amal George Kurian, Shreyas Kumar Jain, Suparna Bhattacharya, Rajendra K Singh, Hae-Won Kim","doi":"10.1186/s12951-025-03614-5","DOIUrl":"10.1186/s12951-025-03614-5","url":null,"abstract":"<p><p>Treating chronic bone injuries and defects remains a significant challenge in orthopedic medicine, impacting patient mobility, recovery time, and healthcare costs. The periosteum, a specialized, vascularized connective tissue covering the outer bone surface, plays a crucial role in osteogenesis and skeletal repair. While regenerating the periosteum is critical to restoring bone structure and function, current treatments face substantial limitations, including limited donor tissue availability, donor site complications, and the risk of immunological rejection. Recent advances in biomaterial engineering have driven the development of therapeutic platforms specifically designed to promote periosteal regeneration. These biomaterial-based platforms mimic the biochemical and biomechanical properties of the native periosteal microenvironment. By facilitating key cellular processes involved in osteogenesis and angiogenesis, these materials enable controlled spatiotemporal delivery of bioactive molecules, ion release, modulation of reactive oxygen species (ROS), and enhancement of pro-angiogenic factors. In this review, we discuss the recent advancements in engineered biomaterials, focusing on their mechanisms of action and applications in periosteum restoration. We also provide insights into current challenges and future research directions, emphasizing the critical role of these strategies in clinical practice. The perspectives offered here aim to guide the development of targeted, effective therapies for periosteum repair, ultimately advancing functional bone regeneration.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"542"},"PeriodicalIF":12.6,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12297785/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144718027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypoxia-augmented chemotherapy potentiates imaging-guided combinatorial radionuclide-sonodynamic therapy for pancreatic cancer.","authors":"Jie An, Kaile Chu, Xirong Li, Huizhu Ma, Qin Zhou, Chenliang Niu, Jie Gao, Junping Lv, Jianbo Cao, XinYu Zhang, Haitao Zhou, Hongliang Wang, Min Li, Zhifang Wu, Sijin Li","doi":"10.1186/s12951-025-03611-8","DOIUrl":"10.1186/s12951-025-03611-8","url":null,"abstract":"<p><p>Radionuclide therapy and chemotherapy are effective for pancreatic cancer, yet their efficacy is often limited by tumor hypoxia. In this study, manganese porphyrin (MnTTP) and tirapazamine (TPZ) were encapsulated in polylactic-co-glycolic acid (PLGA) spheres, which were subsequently coated with polydopamine to label the radionuclide ¹³¹I, forming a theranostic nanoplatform. The nanoplatform demonstrated excellent biocompatibility, stable labeling efficiency, and dual-modal MRI/SPECT imaging capabilities. The nanoplatform generated reactive oxygen species (ROS) under ultrasound(US) activation, in combination with the β-rays emitted by ¹³¹I, synergistically eradicate tumor cells and exacerbate hypoxia in the tumor microenvironment. Furthermore, TPZ was activated to produce toxic free radicals under hypoxic conditions, enabling a synergistic therapeutic approach that combined radionuclide therapy and sonodynamic therapy. This approach effectively inhibited tumor stem cell formation and enhanced anti-tumor efficacy. Additionally, the nanoplatform's metabolism in vivo and the therapeutic effect were monitored in real-time under MRI/SPECT dual-modality imaging. This therapeutic strategy offers a promising solution for overcoming tumor hypoxia and achieving efficient combination therapy for tumors.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"539"},"PeriodicalIF":12.6,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12288203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144707787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Internalized polystyrene nanoplastics trigger testicular damage and promote ferroptosis via CISD1 downregulation in mouse spermatocyte.","authors":"Jing Lv, Guangyu Liu, Ziqi Wang, Jueshun Zhang, Yuanyou Li, Yifan Wang, Ning Liu, Shayakhmetova Altyn, Zhongliang Jiang","doi":"10.1186/s12951-025-03620-7","DOIUrl":"10.1186/s12951-025-03620-7","url":null,"abstract":"<p><strong>Background: </strong>There is a growing body of research regarding the potential reproductive toxicity of microplastics and nanoplastics. However, the underlying mechanisms by which nanoplastics exposure adversely affects the testes remain poorly understood. Our study aims to clarify the relationship between ferritinophagy and mitochondrial dysfunction based on polystyrene nanoplastics (PS-NPs)-caused testicular damage in mice.</p><p><strong>Results: </strong>The current study demonstrates that 50 nm PS-NPs accumulate in mouse testes and lead to a decrease in sperm quality and disruption of spermatocyte. Furthermore, PS-NPs trigger ferroptosis in GC-2 cells, which can be mitigated by deferiprone and 3-methyladenine. Further investigation reveals that PS-NPs initially aggregate in lysosomes and subsequently transfer to the mitochondria. This process increases mitochondrial Fe²⁺ and mitochondrial ROS levels, as well as reduces the expression of CISD1, a protein that inhibits the uptake and transport of Fe²⁺ into the mitochondrial matrix. These changes ultimately result in disturbances to mitochondrial structure and function. In terms of mechanism, pioglitazone, a drug that stabilizes CISD1, has been demonstrated to mitigate ferroptosis induced by NCOA4-mediated ferritinophagy in GC-2 cells.</p><p><strong>Conclusions: </strong>Our results indicate that PS-NPs cause mouse testicular damage through ferroptosis. Mechanistically, we confirmed that PS-NPs trigger NCOA4-mediated ferritinophagy and CISD1 downregulation in spermatocyte, which aggravates the flow of ferrous iron from the cytoplasm to the mitochondria.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"537"},"PeriodicalIF":12.6,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12288249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144707788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive review of unlocking the potential of lignin-derived biomaterials: from lignin structure to biomedical application.","authors":"Jixiang Zhao, Minyu Zhu, Wei Jin, Jinlan Zhang, Guangyu Fan, Yifan Feng, Zhuo Li, Siming Wang, Jung Seung Lee, Guangxiang Luan, Zhengqi Dong, Ying Li","doi":"10.1186/s12951-025-03604-7","DOIUrl":"10.1186/s12951-025-03604-7","url":null,"abstract":"<p><p>Inspired by natural organisms, biomimetic materials with exceptional biocompatibility, degradability, and multifunctionality have emerged as promising candidates for biomedical applications. Lignin, a plant-derived organic polymer, has gained attention due to its intrinsic antioxidant activity, adhesive properties, and biocompatibility. Despite its structural advantages, challenges in stability, biodegradability, and practical implementation hinder its utilization. Structural modifications through chemical/physical treatments or microbial/enzymatic can optimize lignin's bioactivity, mechanical strength, and adhesion, enabling applications in drug delivery, Ultraviolet (UV) shielding, sensing, and wound healing. This review outlines lignin sources, modification principles, and adhesion of biomaterials mechanisms, while showcasing innovative lignin-based materials in biomedical contexts. We highlight their roles in therapeutic delivery systems, tissue engineering & regenerative medicine, and functional biomedical devices, emphasizing lignin's low toxicity and environmental adaptability. By addressing current limitations in processing techniques and clinical translation, we discuss lignin's potential to bridge laboratory research and practical medical solutions. The analysis concludes with an evaluation of lignin's untapped value in sustainable biomedicine, proposing strategies to overcome scalability and standardization barriers. This synthesis provides critical insights for advancing lignin-based technologies toward clinical implementation while maintaining ecological sustainability.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"538"},"PeriodicalIF":12.6,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12288329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144707786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CuOTeDsP nanotherapeutics enhance cuproptosis-mediated immunotherapy by modulating cholesterol metabolism in bladder cancer.","authors":"Hai Cai, Panpan Xue, Siyuan Liu, Yaxin Ma, Xuemei Zeng, Shuangqian Yan, Ning Xu","doi":"10.1186/s12951-025-03609-2","DOIUrl":"10.1186/s12951-025-03609-2","url":null,"abstract":"","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"534"},"PeriodicalIF":12.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12281780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A fibrin gel-loaded Gouqi-derived nanovesicle (GqDNV) repairs the heart after myocardial infarction by inhibiting p38 MAPK/NF-κB p65 pathway.","authors":"Huan-Huan Zhou, Xiaolei Zhou, Jianqiu Pei, Shiyin Xu, Biyu Jin, Jiuling Chen, Zixuan Zhang, Mingmeng Tang, Yan Liu, Andreas K Nüssler, Liegang Liu, Qin Xu, Anxin Wang, Min Xia, Wei Yang","doi":"10.1186/s12951-025-03615-4","DOIUrl":"10.1186/s12951-025-03615-4","url":null,"abstract":"<p><p>The restoration of cardiac function post-myocardial infarction (MI) remains a significant clinical challenge. Emerging evidence indicates that Goji berries (\"Gouqi\" in Chinese) and their extracts exhibit substantial cardioprotective properties. Here, we introduce fibrin gel-loaded Gouqi-derived nanovesicles (GqDNVs-gel) as a delivery system targeted at the infarcted myocardium. The application of GqDNVs-gel resulted in a marked improvement in survival rates over a 14-day period post-MI, enhanced cardiac function, reduced infarct size, myocardial apoptosis, and excessive fibrosis, and facilitated endogenous repair. Through a combination of transcriptomics and proteomics analyses, alongside in vitro and in vivo experiments, we identified that the cardioprotective effect of GqDNVs are mediated through the inhibition of the p38 MAPK-NF-κB p65 signaling pathway. Furthermore, GqDNVs contain abundant bioactive compounds, including proteins, genetic materials, lipids, polysaccharides, and flavonoids. GqDNVs-gel intervention can reshape the post-MI cardiac environment and modulate myocardial lipid metabolism, specifically impacting glycerophospholipid and α-linolenic acid metabolic pathways. The upregulation of the peptide Arg-Thr-Ile-Glu and the downregulation of phosphatidylethanolamine in the hearts of MI mice after GqDNVs-gel intervention may play crucial roles in modulating the associated metabolic pathways. This study is the first to highlight the multifaceted therapeutic effects of GqDNVs-gel, offering a promising strategy for enhancing cardiac function post-MI.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"535"},"PeriodicalIF":12.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12281781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}