Journal of Nanobiotechnology最新文献

{"title":"Stimuli-responsive smart materials enabled high-performance biosensors for liquid biopsies.","authors":"Xiaoqi Gao, Bayinqiaoge Bayinqiaoge, Ming Li, Rona Chandrawati, Xiangpeng Li, Lining Sun, Chun H Wang, Chengchen Zhang, Shi-Yang Tang","doi":"10.1186/s12951-025-03541-5","DOIUrl":"https://doi.org/10.1186/s12951-025-03541-5","url":null,"abstract":"<p><p>Liquid biopsies have emerged as a key tool that enables personalized medicine, enabling precise detection of biochemical parameters to tailor treatments to individual needs. Modern biosensors enable real-time detection, precise diagnosis, and dynamic monitoring by rapidly analyzing biomarkers such as nucleic acids, proteins, and metabolites in bodily fluids like blood, saliva, and urine. Despite their potential, many biosensors are still constrained by mono-functionality, sub-optimal sensitivity, bulky designs, and complex operation requirements. Recent advances in stimuli-responsive smart materials present a promising pathway to overcome these limitations. These materials enhance biomarker signal transduction, release, or amplification, leading to improved sensitivity, simplified workflows, and multi-target detection capabilities. Further exploration of the integration of these smart materials into biosensing is therefore essential. To this end, this review critically examines and compares recent progress in the development and application of physical, chemical, and biochemical stimuli-responsive smart materials in biosensing. Emphasis is placed on their responsiveness mechanisms, operational principles, and their role in advancing biosensor performance for biomarker detection in bodily fluids. Additionally, future perspectives and challenges in developing versatile, accurate, and user-friendly biosensors for point-of-care and clinical applications using these smart materials are discussed.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"477"},"PeriodicalIF":10.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early-life exposure to polypropylene nanoplastics induces neurodevelopmental toxicity in mice and human iPSC-derived cerebral organoids.","authors":"Fangfang Huang, Haizhen You, Xiaogang Tang, Yuantao Su, Huijuan Peng, Huizhen Li, Zhiyun Wei, Jing Hua","doi":"10.1186/s12951-025-03561-1","DOIUrl":"https://doi.org/10.1186/s12951-025-03561-1","url":null,"abstract":"<p><p>Nanoplastics (NPs) are emerging environmental pollutants that pose growing concerns due to their potential health risks. However, the effects of inhaled NP exposure during pregnancy on fetal brain development remain poorly understood. In this study, we investigated the impact of maternal exposure to polypropylene nanoplastics (PP-NPs) on fetal brain development and neurobehavioral outcomes in a mouse model and further explored its mechanism in human cerebral organoids. Maternal exposure to PP-NPs significantly impaired neuronal differentiation and proliferation in the fetal cortex. Neurobehavioral assessments revealed significant deficits in offspring following maternal exposure, including impaired spatial memory, reduced motor coordination, and heightened anxiety-like behavior. Furthermore, human brain organoids exposed to PP-NPs exhibited reduced growth and neuronal differentiation, with significant downregulation of key neuronal markers such as TUJ1, MAP2, and PAX6. Transcriptomic analysis identified alterations in gene expression, particularly in neuroactive ligand-receptor interaction pathway. Molecular docking and fluorescence co-localization analysis further suggested CYSLTR1 and PTH1R as key molecular targets of PP-NPs. These findings provide novel insights into the toxicological effects of NPs on the developing brain and emphasize the need for preventive measures to protect fetal neurodevelopment during pregnancy.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"474"},"PeriodicalIF":10.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cancer cell membrane-camouflaged pH-responsive nanoparticles for enhancing siRNA effect and synergistic anti-tumor therapy.","authors":"Jie Zhang, Yun Peng Zhang, Qi Sun, Yaoqi Wang, Dong Mei, Xiaoling Wang, Yan Su, Yang Tian, Ran Huo, Danni Liu, Siyu Liu, Myagmarsuren Baldan, Shuang Zhang, Chunying Cui","doi":"10.1186/s12951-025-03508-6","DOIUrl":"https://doi.org/10.1186/s12951-025-03508-6","url":null,"abstract":"<p><p>RNA-based therapies, especially small interfering RNA (siRNA), have attracted extensive attention for tumor treatment. However, most siRNA can't exert a therapeutic effect due to a lack of targeting to tumor cells and entrapment in lysosomes upon administration. To address the challenges associated with siRNA delivery, a delivery system was developed using zinc oxide nanoparticles (ZnO NPs) coated with cancer cell membranes. ZnO nanoparticles (ZnO NPs) have been recognized as effective pH-responsive nanoparticles and are widely used in the development of pH-responsive drug delivery systems. The ZnO NPs were combined with chitosan to encapsulate siRNA, allowing for dissolution in acidic lysosomes and the subsequent release of siRNA and chitosan complexes. The dissolution of ZnO NPs would also disrupt lysosomes, facilitating the escape of siRNA and enhancing its gene silencing effect. However, the chitosan and ZnO NPs nano-complexes (CS/ZnO@siRNA) were unstable in solution and lacked a specific targeting effect for tumor cells. Thus, a homologous cancer cell membrane was coated onto the nanoparticles, which has been shown to be an effective strategy for enhancing their stability and targeting capabilities. Moreover, ZnO NPs not only dissolve in acidic lysosomes to enhance the efficacy of siRNA but also elevate oxidative stress levels in cells, leading to the induction of cellular apoptosis. It was demonstrated both in vitro and in vivo that the ZnO NPs could synergistically combine with the anti-tumor siRNA (siSurvivin) to inhibit the growth of the 4T1 tumor. Altogether, the developed drug delivery system (CCM-CS/ZnO@siSurvivin) offers a new strategy for enhancing the therapeutic effect of siRNA, while synergistically inhibiting tumor growth.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"471"},"PeriodicalIF":10.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel adjuvant delivery system constructed by alum-emulsion hybrid nanoparticles with TLR9 agonists boosts vaccine immunity.","authors":"Yarong Zeng, Yifan Yin, Jialin Xu, Rong Su, Sibo Zhang, Feng Han, Yufang Li, Xueqing Zhu, Ciying Qian, Feihong Zou, Renfei Gao, Xiuli Zhang, Lizhi Zhou, Tingting Li, Min Lin, Qingbing Zheng, Hai Yu, Jun Zhang, Zizheng Zheng, Ying Gu, Ningshao Xia, Shaowei Li","doi":"10.1186/s12951-025-03560-2","DOIUrl":"https://doi.org/10.1186/s12951-025-03560-2","url":null,"abstract":"<p><p>Vaccine Adjuvant Delivery Systems (VADSs) that integrate both delivery and adjuvancy constitute a vital strategy in modern vaccine research. Herein, we employed microfluidics to blend nanosized aluminum hydroxide (XA) and a nanoemulsion (XE) in varying proportions. A 2:3 (v/v) mixture produced nanoparticles (XAE) of approximately 220 nm with near-neutral zeta potential, which significantly enhanced antigen uptake in DC2.4 cells and displayed stronger adjuvant activity than XA or XE alone. By electrostatically adsorbing the TLR9 agonist CpG onto XAE, we constructed XAEC as an integrated delivery platform that co-delivers antigens and immune-stimulatory molecules, thereby improving CpG's bioavailability and biocompatibility. This XAEC platform generated an \"immune-activated microenvironment\" at the injection site, fostering antigen-presenting cell (APC) recruitment, augmenting antigen presentation, facilitating vaccine trafficking to immune organs, and eliciting a Th1-biased immune response. XAEC substantially boosted immunogenicity in prophylactic Respiratory Syncytial Virus (RSV) and Varicella-Zoster Virus (VZV) vaccines and induced potent antitumor responses in a Human Papillomavirus (HPV) tumor model. Hence, XAEC emerges as a promising next-generation vaccine adjuvant system.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"472"},"PeriodicalIF":10.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brain targeted lipid nanoparticles with Hv1 inhibitors alleviate neuroinflammation post-ischemic stroke.","authors":"Zeyu Yang, Lei Jin, Longxiang Li, Yu Wu, Wenchao Liu, Xin Feng, Liyan Li, Fa Jin, Yiming Bi, Ran Li, Shenquan Guo, Yanan Wang, Boyang Wei, Yanchao Liu, Xifeng Li, Chuanzhi Duan","doi":"10.1186/s12951-025-03540-6","DOIUrl":"https://doi.org/10.1186/s12951-025-03540-6","url":null,"abstract":"<p><strong>Background: </strong>Ischemic stroke (IS) represents a significant global health burden. Current therapeutic options face problems such as window narrowing and reperfusion injury risk. Moreover, with increasing aging and risk factors, novel treatment strategies are urgently needed. NADPH oxidase (NOX)-mediated oxidative stress in microglia is a critical mechanism driving neuroinflammation and cell death. Hv1, a voltage-gated proton channel highly expressed in microglia, synergizes with NOX to generate reactive oxygen species (ROS), exacerbating brain damage. YHV984, a potent Hv1 inhibitor, alleviates post-IS neuroinflammation but faces clinical limitations due to potential toxic side effects and solubility issues. To improve the physicochemical and pharmacokinetic properties of YHV984 for specific Hv1 inhibition in the brain, the multifunctional nanoparticles consisting of a T7-targeting peptide and lipid nanoparticles (LNP) were developed to deliver YHV984 (T7-LNP@YHV984).</p><p><strong>Results: </strong>The results demonstrated that T7-LNP@YHV984 exhibited good stability and brain targeting capability, effectively crossing the blood-brain barrier (BBB) and accumulating within microglia. This targeted delivery significantly suppressed Hv1 expression and activation of the NLRP3 inflammasome pathway in the damaged brain. Furthermore, it promoted the polarization of microglia towards the M2 phenotype, enhancing the release of anti-inflammatory factors, alleviating neuroinflammation and improved neuronal survival. Additionally, T7-LNP@YHV984 improved survival and facilitated neurological recovery in post-IS mice.</p><p><strong>Conclusions: </strong>T7-LNP@YHV984 multifunctional nanoparticles with long-term stability emerged as a potent strategy to alleviate reperfusion injury and inhibit neuroinflammation post-IS. By precisely targeting Hv1 in microglia, the nanoparticles effectively suppressed microglia-induced neuroinflammation, minimizing off-target effects. This innovation offers novel insights into stroke treatment and neuroprotective strategies.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"464"},"PeriodicalIF":10.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel carrier-free nanoparticle with stable distinctive three-dimensional structure for tumor-targeted precision chemoimmunotherapy.","authors":"Bo Deng, Youpeng Kong, Yingying Ma, Yijie Zhan, Yitong Sun, Rudan Wang, Pengyu Huang, Lanxia Liu","doi":"10.1186/s12951-025-03568-8","DOIUrl":"https://doi.org/10.1186/s12951-025-03568-8","url":null,"abstract":"<p><p>Despite significant advancements in oncology, cancer remains a leading global health burden, necessitating innovative therapeutic strategies. Here, we present a novel carrier-free tumor-targeted nanomedicine system (DPA NPs) for tumor-targeted chemoimmunotherapy, formed by self-assembly of a conjugate synthesized with doxorubicin (DOX), tumor-homing peptide iRGD, matrix metalloproteinase 2 enzyme responsive peptide (MMP2), and adjuvant monophosphoryl lipid A (MPLA). The results demonstrated that DPA NPs exhibited a stable unique 3D nanostructure with tumor microenvironment (TME)-responsive properties. DPA NPs could efficiently deliver DOX to tumor cells, inducing immunogenic cell death (ICD) and simultaneously triggering tumor specific immune response. Meanwhile, MPLA amplified the anti-tumor immunity, significantly inhibiting tumor growth and metastasis. When combined with immune checkpoint inhibitors (ICIs), DPA NPs further enhanced the therapeutic outcomes in a B16 melanoma model, demonstrating remarkable suppression of tumor growth, metastasis inhibition and recurrence prevention. Mechanistic investigations across multiple biological hierarchies conclusively further confirmed the synergistic therapeutic effect. This study demonstrated that DPA NPs provide a precise, multifunctional nanoplatform for tumor-targeted combination therapy, highlighting their potential for clinical translation in cancer treatment.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"480"},"PeriodicalIF":10.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanomaterial isolated extracellular vesicles enable high precision identification of tumor biomarkers for pancreatic cancer liquid biopsy.","authors":"Zachary F Greenberg, Samantha Ali, Andrew Brock, Jinmai Jiang, Thomas D Schmittgen, Song Han, Steven J Hughes, Kiley S Graim, Mei He","doi":"10.1186/s12951-025-03527-3","DOIUrl":"https://doi.org/10.1186/s12951-025-03527-3","url":null,"abstract":"<p><p>Pancreatic cancer has the highest mortality rate among all major cancers, which highlights the urgent needs of non-invasive early detection. Circulating extracellular vesicles (EVs) have gained significant attention for discovering tumor biomarkers. However, isolating EVs with well-defined homogeneous populations from complex biological samples is challenging. Different methods have been found to derive different EV populations carrying different biomolecules, which significantly confound biomarker discovery for developing clinical diagnostics. Building a rigorous EV isolation and standardizing assessment platform associated with -omics is essential to overcome this challenge. We introduced a novel isolation approach using a pH-responsive peptide conjugated with NanoPom magnetic beads (ExCy) for homogeneous EV isolation. Additionally, we introduced the first statistical algorithm for EV quality assessment (ExoQuality Index, EQI), which enables multi-assay quantification to provide a consistent and accurate definition of EV purity and quality; ExoQuality's algorithm intakes multi-assay information to deconvolute complex EV heterogeneity. We performed the next generation sequencing on EV RNAs from pancreatic cancer patient plasma using four isolation methods; results highlighting ExCy's isolation and EQI assessment improved biomarker identification. We identified a novel EV biomarker for pancreatic tumor, ATP6V0b, validated with quantitative PCR (qPCR) by screening a pilot cohort of 22 plasma samples. 16 were from pancreatic cancer patients, 6 with matched tumor tissue, and 6 healthy plasma samples. Through modelling the ATP6V0B cycling threshold, we reported 3 models with AUCs between 0.86 and 0.88, showcasing an enabling and clinically translatable liquid biopsy approach for early detection of pancreatic cancer using circulating EVs.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"467"},"PeriodicalIF":10.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural small molecule smart hydrogels inhibited the Hsp90/NF-κB signaling axis in inflammation to achieve sustained antipyretic effect.","authors":"Yuqin Yang, Jingyi Jiao, Xiaohui Jia, Lei Li, Meiling Wu, Xinyue Lu, Yangyang Sun, Yingqi Lang, Fuhao Chu, Dong Bai, Penglong Wang, Haimin Lei","doi":"10.1186/s12951-025-03517-5","DOIUrl":"https://doi.org/10.1186/s12951-025-03517-5","url":null,"abstract":"<p><p>In general, pseudoephedrine (PE) is a safe and universally employed in cold medicine, which displays powerful effect on antipyretic. Nonetheless, the sustained drug delivery system can effectively put an end to the above problems attributable to the drawbacks of low bioavailability and short intervals of administration. \"Complexation\" hydrogels are capturing enormous attention in a diverse array of fields in that there is no necessity to carry out external intervention for drug delivery. Nevertheless, it is prevalently acknowledged that macromolecular \"complexation\" hydrogels, biotin/avidin, antibodies/antigens, heterodimers, conA/glucose and cyclodextrin (CD) inclusion complexes, have several limitations of conventional drug delivery systems, such as unfavorable biological safety, undesirable intestinal wall penetrating, and extremely limited biodegradability, etc. For this reason, it is tremendously imperative to develop a natural small \"complexation\" hydrogel. In this context, we innovated a direct self-assembly \"complexation\" hydrogel (PE-GA). The PE-GA hydrogel was prepared by the incorporation of PE and glycyrrhizic acid (GA) into an aqueous dispersion without the aid of other carriers, which demonstrated dual-responsiveness including heating-cooling as well as pH. It is mainly governed by hydrogen bonds and electrostatic interactions. For cell bioavailability, there were substantial discrepancies between the PE-GA hydrogel and free PE at 72 and 84 h. For pharmacokinetic properties, there was also conspicuous discrepancy in Area Under the Curve (AUC) values between them. In subsequent antipyretic assay, PE-GA hydrogel displayed a conspicuous antipyretic effect in fever rats induced by LPS. The non-invasive fluorescence imaging was utilized to monitor the intestinal retention of the PE-GA hydrogel in mice, its unique aggregation/assembly induced retention (AIR) effect reinforced bioactive molecule retention, which may be another manifestation of enhancing antipyretic effect. Aside from that, PE-GA hydrogel played an antipyretic role by Hsp90/NF-κB pathway. The current research revealed potential antipyretic effect of PE-GA hydrogel which could be the therapeutic option against fever.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"478"},"PeriodicalIF":10.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemotherapeutic drug scavenger-based combination therapy toward treating triple-negative breast cancer.","authors":"Qingqing Yin, Yutong Zhong, Mengchun Chen, Weian Mao, Yuan Yang, Li Li, Dongyan Tian, Shuangshuang Liu, Ying Chen, Jiale Quan, Shiyu Li, Deli Zhuge, Xufei Zhang, Ledan Wang, Fang Wang, Yiming Chen, Xiaosheng Lu, Xiaoji Lin, Yijie Chen, Linzhi Yan","doi":"10.1186/s12951-025-03571-z","DOIUrl":"https://doi.org/10.1186/s12951-025-03571-z","url":null,"abstract":"<p><p>Triple-negative breast cancer (TNBC) is resistant to most antitumor treatments, leaving chemotherapy as the primary option. Although doxorubicin (Dox) in combination with other therapies is promising for TNBC management, the combined effect is still compromised by the dose-limiting toxicities of Dox. Here, we developed a chemotherapeutic drug scavenger (CDS) by encapsulating GC-rich DNA-preferred binding targets of Dox-within an erythrocyte membrane functionalized with a normal tissue-targeting (NTT) peptide. Mimicking the structure of the cell nucleus, CDS selectively absorbs and neutralizes Dox in susceptible normal organs while sparing tumor tissues. This targeted detoxification allows for safe escalation of the Dox dose to 15 mg/kg, three times the standard 5 mg/kg, without observable toxicity. Such a high Dox dose enabled by CDS pretreatment significantly inhibited the post-operative residual/metastasized 4T1 tumor growth, regardless of the early or later stages of the tumor. Also, delivery of a high dose of Dox into the 4T1 tumor could profoundly increase the G2/M arrest, facilitating the combination therapy with a low-powered radiation of 2 Gy. Further, tumor exposure to high Dox amounts could convert the 4T1 tumor microenvironment from 'cold' to 'hot', leading to improved infiltration of immune cells, including T cells, dendritic cells, and macrophages. Overall, this study demonstrates how the safe injection of high amounts of Dox enabled by CDS detoxification could augment and extend Dox's functionality combined with surgery, radiotherapy, and cell therapy for TNBC treatment.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"473"},"PeriodicalIF":10.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Normal stress on surface of mesenchymal stem cells boosts extracellular vesicle secretion and regenerative bioactivity.","authors":"Chengmeng Bai, Huifang Ren, Feng Zhang, Dandan Li, Qingyu Feng, Ying Li, Ting Chen, Chengxu Li, Xuan Xiao, Hengrui Zhang, Liang Hu","doi":"10.1186/s12951-025-03556-y","DOIUrl":"https://doi.org/10.1186/s12951-025-03556-y","url":null,"abstract":"<p><p>Scalable approaches for enhancing therapeutic small extracellular vesicles (sEVs) production can facilitate the transition of sEVs from bench to bedside and beyond. Here, we present a user-friendly method to manipulate the extracellular mechanical microenvironment of umbilical cord-derived mesenchymal stem cells (MSCs), a promising cell type for generating sEVs with therapeutic benefits, to boost sEV secretion and regenerative bioactivity. The bioreactor system, called the programmable controlled rotating platform (PRP), is designed to apply normal stress on cell surface through centrifugal rotation culture. Experimental analyses suggested that the PRP can promote a 4-fold sEV secretion increase without affecting cell viability and sEV size when compared to the traditional static culture condition. More importantly, PRP-induced MSC-sEVs can significantly promote epithelial cell migration in vitro and accelerate corneal wound healing in a murine model, with suppressed inflammatory responses in wound bed tissue. Further mechanistic investigations revealed that this process involves the activation of cellular transcriptional signals implicated in sEV biogenesis. Concurrently, sEV cargo undergoes remodeling to enrich regenerative and immunoregulatory functions. These findings demonstrate the efficacy of our established platform in advancing sEV production and improving clinical performance, providing a novel sEV-based mechanism for ocular treatments, including corneal epithelialization and even retinal neural regeneration.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"476"},"PeriodicalIF":10.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}