Journal of Nanobiotechnology最新文献

CuOTeDsP nanotherapeutics enhance cuproptosis-mediated immunotherapy by modulating cholesterol metabolism in bladder cancer. CuOTeDsP纳米治疗药物通过调节膀胱癌胆固醇代谢来增强铜中毒介导的免疫治疗。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2025-07-22 DOI: 10.1186/s12951-025-03609-2

Hai Cai, Panpan Xue, Siyuan Liu, Yaxin Ma, Xuemei Zeng, Shuangqian Yan, Ning Xu

引用次数: 0

A fibrin gel-loaded Gouqi-derived nanovesicle (GqDNV) repairs the heart after myocardial infarction by inhibiting p38 MAPK/NF-κB p65 pathway. 一种纤维蛋白凝胶负载的沟气衍生纳米囊泡（GqDNV）通过抑制p38 MAPK/NF-κB p65途径修复心肌梗死后的心脏。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2025-07-22 DOI: 10.1186/s12951-025-03615-4

Huan-Huan Zhou, Xiaolei Zhou, Jianqiu Pei, Shiyin Xu, Biyu Jin, Jiuling Chen, Zixuan Zhang, Mingmeng Tang, Yan Liu, Andreas K Nüssler, Liegang Liu, Qin Xu, Anxin Wang, Min Xia, Wei Yang

{"title":"A fibrin gel-loaded Gouqi-derived nanovesicle (GqDNV) repairs the heart after myocardial infarction by inhibiting p38 MAPK/NF-κB p65 pathway.","authors":"Huan-Huan Zhou, Xiaolei Zhou, Jianqiu Pei, Shiyin Xu, Biyu Jin, Jiuling Chen, Zixuan Zhang, Mingmeng Tang, Yan Liu, Andreas K Nüssler, Liegang Liu, Qin Xu, Anxin Wang, Min Xia, Wei Yang","doi":"10.1186/s12951-025-03615-4","DOIUrl":"https://doi.org/10.1186/s12951-025-03615-4","url":null,"abstract":"The restoration of cardiac function post-myocardial infarction (MI) remains a significant clinical challenge. Emerging evidence indicates that Goji berries (\"Gouqi\" in Chinese) and their extracts exhibit substantial cardioprotective properties. Here, we introduce fibrin gel-loaded Gouqi-derived nanovesicles (GqDNVs-gel) as a delivery system targeted at the infarcted myocardium. The application of GqDNVs-gel resulted in a marked improvement in survival rates over a 14-day period post-MI, enhanced cardiac function, reduced infarct size, myocardial apoptosis, and excessive fibrosis, and facilitated endogenous repair. Through a combination of transcriptomics and proteomics analyses, alongside in vitro and in vivo experiments, we identified that the cardioprotective effect of GqDNVs are mediated through the inhibition of the p38 MAPK-NF-κB p65 signaling pathway. Furthermore, GqDNVs contain abundant bioactive compounds, including proteins, genetic materials, lipids, polysaccharides, and flavonoids. GqDNVs-gel intervention can reshape the post-MI cardiac environment and modulate myocardial lipid metabolism, specifically impacting glycerophospholipid and α-linolenic acid metabolic pathways. The upregulation of the peptide Arg-Thr-Ile-Glu and the downregulation of phosphatidylethanolamine in the hearts of MI mice after GqDNVs-gel intervention may play crucial roles in modulating the associated metabolic pathways. This study is the first to highlight the multifaceted therapeutic effects of GqDNVs-gel, offering a promising strategy for enhancing cardiac function post-MI.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"535"},"PeriodicalIF":10.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quercetin combined with shTERT induces apoptosis in ovarian cancer via the P53/Bax pathway, and RGD-MSN/QR/shTERT nanoparticles enhance the therapeutic efficacy. 槲皮素联合shTERT通过P53/Bax通路诱导卵巢癌细胞凋亡，RGD-MSN/QR/shTERT纳米颗粒增强了治疗效果。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2025-07-22 DOI: 10.1186/s12951-025-03546-0

Guojie Chen, Weiwei Song, Xing Wang, Guangyao Mao, Weifeng Hu, Rongrong Dou, He Zhu, Yongkang Zhang, Xianhua Fu, Mei Lin

{"title":"Quercetin combined with shTERT induces apoptosis in ovarian cancer via the P53/Bax pathway, and RGD-MSN/QR/shTERT nanoparticles enhance the therapeutic efficacy.","authors":"Guojie Chen, Weiwei Song, Xing Wang, Guangyao Mao, Weifeng Hu, Rongrong Dou, He Zhu, Yongkang Zhang, Xianhua Fu, Mei Lin","doi":"10.1186/s12951-025-03546-0","DOIUrl":"https://doi.org/10.1186/s12951-025-03546-0","url":null,"abstract":"Background: Ovarian cancer (OC) is a highly malignant gynecological tumor with poor current treatment effects. Telomerase reverse transcriptase (TERT) is an important component of telomerase and plays an important role in the progression of ovarian cancer. Quercetin(QR) has been shown to inhibit the cell cycle and induce the apoptosis in various types of tumors. However, the mechanism of quercetin in ovarian cancer and whether it can be applied in the treatment of ovarian cancer has not been fully understood.Results: OC cells were intervened with QR in vitro and it was found that QR only inhibited the cell cycle but not induced cell apoptosis. By conducting network pharmacology, proteomics and TCGA-OV database analysis, we found that QR inhibited the cell cycle by binding to P53 and P21. However, in this study, overexpressed TERT in OC could bind to P53 and inhibit the binding of QR to P53, failing to induce tumor cell apoptosis. After TERT was knocked down, QR significantly suppressed the cell cycle of OC cells and induced apoptosis.To realize high drug delivery efficiency and drug targeting to improve the effect of inhibiting OC, we designed and prepared RGD-MSN/QR/shTERT nanoparticles for the combined administration of QR and shTERT. As confirmed by the in vivo experiments, RGD-MSN/QR/shTERT possessed good targeting ability and significant OC inhibiting effect, with no adverse reactions, and improved the survival benefits.Conclusions: This study demonstrated the mechanistic and therapeutic advantages of combining QR with shTERT in the treatment of OC. Based on this mechanism, we synthesized the novel nanoparticles (RGD-MSN/QR/shTERT) and verified the favorable OC inhibiting effect in vivo, providing a novel strategy for the treatment of OC.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"536"},"PeriodicalIF":10.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifunctional nanoagent for enhanced cancer radioimmunotherapy via pyroptosis and cGAS-STING activation. 通过焦亡和cGAS-STING激活增强癌症放射免疫治疗的多功能纳米剂。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2025-07-21 DOI: 10.1186/s12951-025-03608-3

Ziting Xu, Yang Gao, Li Zhang, Yingshan Gao, Yushu Liao, Yu Liang, Zhen Yuan, Yingjia Li, Bingxia Zhao, Ge Wen

{"title":"Multifunctional nanoagent for enhanced cancer radioimmunotherapy via pyroptosis and cGAS-STING activation.","authors":"Ziting Xu, Yang Gao, Li Zhang, Yingshan Gao, Yushu Liao, Yu Liang, Zhen Yuan, Yingjia Li, Bingxia Zhao, Ge Wen","doi":"10.1186/s12951-025-03608-3","DOIUrl":"10.1186/s12951-025-03608-3","url":null,"abstract":"The immunosuppressive tumor microenvironment (ITME) and inherent radioresistance of tumor cells limit the effectiveness of radioimmunotherapy and exacerbate immune evasion. To address these challenges, PEGylated Azacitidine-loaded and Mn2+-doped calcium carbonate nanoparticles (A@MCP NPs) are synthesized as multifunctional nanoagent to enhance radioimmunotherapy outcomes. Upon acidic TME, the release of Ca2+ and Mn2+ from A@MCP NPs co-triggers intracellular reactive oxygen species (ROS) generation via Ca2+ overload and Fenton-like reactions, inducing cytochrome C release and caspase-3 activation. Concurrently, released Azacitidine inhibits DNA methylation, upregulating GSDME expression in irradiated tumor cells, which synergistically amplifies caspase-3/GSDME-induced pyroptosis. The resulting pyroptotic cell damage, coupled with radiotherapy (RT)-induced DNA, activates Mn2+-sensitized cGAS-STING pathways, amplifying immune responses. Collectively, A@MCP, as a nano radiosensitizer, together with RT, co-activates pyroptosis and cGAS-STING to further amplify anti-tumor immune response, overcome ITME-mediated resistance and offer significant potential for improved cancer radioimmunotherapy.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"527"},"PeriodicalIF":10.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12278542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metal-organic nanostructures based on sono/chemo-nanodynamic synergy of Ti_xO_y/Ru reaction units: for ultrasound-induced dynamic cancer therapy. 基于声/化学- TixOy/Ru反应单元纳米动态协同作用的金属有机纳米结构：用于超声诱导的动态癌症治疗。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2025-07-21 DOI: 10.1186/s12951-025-03599-1

Tao Jiang, Zixiang Tang, Shumiao Tian, Haitian Tang, Zhekun Jia, Fangjian Li, Chenyue Qiu, Lin Deng, Lang Ke, Pan He, Gang Liu, Chengchao Chu, Yongfu Xiong

{"title":"Metal-organic nanostructures based on sono/chemo-nanodynamic synergy of TixOy/Ru reaction units: for ultrasound-induced dynamic cancer therapy.","authors":"Tao Jiang, Zixiang Tang, Shumiao Tian, Haitian Tang, Zhekun Jia, Fangjian Li, Chenyue Qiu, Lin Deng, Lang Ke, Pan He, Gang Liu, Chengchao Chu, Yongfu Xiong","doi":"10.1186/s12951-025-03599-1","DOIUrl":"10.1186/s12951-025-03599-1","url":null,"abstract":"Sonodynamic therapy (SDT) exhibits clinical potential for deep-tissue tumor treatment due to its deep tissue penetration and spatiotemporal controllability. Its core mechanism relies on ultrasound-activated sonosensitizers to generate reactive oxygen species (ROS), thereby inducing tumor cell apoptosis. However, conventional sonosensitizers face limitations in ROS yield and tumor-targeting efficiency. In this study, we innovatively designed a multifunctional metal-organic nanosheet (TiZrRu-MON) by hydrothermal coordination of [Ru(bpy)₃]2⁺ photosensitizing units with TiZr-O clusters, while incorporating Fe3⁺ to construct a cascade catalytic system. Experimental results demonstrated that: (1) Fe3⁺ lattice doping significantly enhanced charge carrier mobility and ultrasound-triggered 1O₂ quantum yield via the formation charge transfer channels; (2) The acidic tumor microenvironment activated Fe3⁺-mediated Fenton reactions, establishing a positive feedback loop with SDT to synergistically amplify ROS generation; (3) Hyaluronic acid functionalization improved nanosheet internalization in HepG2 tumor cells through CD44 receptor-mediated endocytosis. Remarkably, ultrasound irradiation induced substantial oxidative stress and immunogenic cell death, promoting the release of damage-associated molecular patterns (DAMPs), which elevated the maturation rate of tumor-infiltrating dendritic cells (DCs) and significantly increased the proportion of CD8⁺ T cells. In a mouse subcutaneous tumor model, the system achieved effective tumor suppression with manageable systemic toxicity. This work proposes a metal-ligand coordination strategy to advance the development of high-performance sonosensitizers and immunomodulatory antitumor technologies.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"533"},"PeriodicalIF":10.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12281788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomimetic phosphorus dendrimer multi-epitope nanovaccine enhances humoral and cellular immune response against African swine fever virus. 仿生磷树突多表位纳米疫苗增强对非洲猪瘟病毒的体液和细胞免疫应答。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2025-07-21 DOI: 10.1186/s12951-025-03593-7

Hong Duan, Aijuan Shen, Min Wang, Fengxia Zhang, Ziheng Zhang, Yaci Zhang, Yunshuo Lu, Qiming Pei, Angke Zhang

{"title":"Biomimetic phosphorus dendrimer multi-epitope nanovaccine enhances humoral and cellular immune response against African swine fever virus.","authors":"Hong Duan, Aijuan Shen, Min Wang, Fengxia Zhang, Ziheng Zhang, Yaci Zhang, Yunshuo Lu, Qiming Pei, Angke Zhang","doi":"10.1186/s12951-025-03593-7","DOIUrl":"10.1186/s12951-025-03593-7","url":null,"abstract":"Using nanoparticles (NPs) as a platform for multivalent antigen display is an effective strategy to increase the immunogenicity of subunit vaccines, which can induce high levels of humoral and cellular immunity. In addition, antigens that target antigen-presenting cells (APCs) can further increase their immunogenicity. To date, there are no commercially available ASFV vaccines available worldwide. The present study developed a dendritic cell (DC)-targeting ASFV biomimetic nanovaccine. First, a high-affinity and specific nanobody (Nb) targeting DCs was screened and expressed in tandem with B and T-cell epitopes of highly immunogenic p30, p54, p72, pB602L, and CD2V proteins of ASFV (Nb-rAg). The Nb-rAg complexes were then loaded onto azabisphosphonate-terminated phosphorus dendrimers (PPHs) to construct PPH-Nb-rAg NPs, which were subsequently coated with ASFV-infected activated porcine alveolar macrophage (PAM) membranes to prepare the PPH-Nb-rAg@PM biomimetic nanovaccine. Finally, the immune efficacy of the nanovaccine was evaluated in mice. Notably, compared with the PBS, rAg, Nb-rAg, and PPH-Nb-rAg immunization groups, the PPH-Nb-rAg@PM immunization group exhibited stronger ASFV antigen-specific humoral and cellular immune responses. Single-cell RNA sequencing (scRNA-seq) revealed that immunization with PPH-Nb-rAg@PM increased the proportions of B cells, T cells, NK cells, plasma cells, and macrophages in the mouse spleen. Further analysis revealed that PPH-Nb-rAg@PM immunization increased the numbers of memory B cells and plasma cells in the mouse spleen, and the numbers of CD4 + T cells, CD8 + T cells and NK cells also increased compared with those in the control group. These results suggest that PPH-Nb-rAg@PM is a promising and effective candidate vaccine against ASFV.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"530"},"PeriodicalIF":10.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12278616/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Injectable HAMA-CPC hydrogels loaded with high-yield 3D bioprinted adipose-derived stem cell small extracellular vesicles for increased bone repair. 可注射的HAMA-CPC水凝胶装载了高产量的3D生物打印脂肪来源的干细胞细胞外小泡，用于增加骨修复。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2025-07-21 DOI: 10.1186/s12951-025-03596-4

Wenbin Xu, Wenling Gao, Yi Zhang, Gang Hou, Wenhui Zhang, Jintao Deng, Kun Wang, Yichun Xu, Boxun Liu, Tao Xu, Chang Liu, Tangzhao Liang

{"title":"Injectable HAMA-CPC hydrogels loaded with high-yield 3D bioprinted adipose-derived stem cell small extracellular vesicles for increased bone repair.","authors":"Wenbin Xu, Wenling Gao, Yi Zhang, Gang Hou, Wenhui Zhang, Jintao Deng, Kun Wang, Yichun Xu, Boxun Liu, Tao Xu, Chang Liu, Tangzhao Liang","doi":"10.1186/s12951-025-03596-4","DOIUrl":"10.1186/s12951-025-03596-4","url":null,"abstract":"In the field of bone tissue engineering, the development of effective strategies for bone defect repair remains a major challenge. Herein, we report a novel approach involving the integration of high-yield 3D bioprinted adipose-derived stem cell small extracellular vesicles (3D-sEVs) into an injectable HAMA-CPC hydrogel (HAMA-CPC@3D-sEVs). In vitro, HAMA-CPC@3D-sEVs significantly promoted the proliferation, migration, and osteogenic differentiation of bone marrow stromal cells (BMSCs) and promoted angiogenesis in human umbilical vein endothelial cells (HUVECs). In vivo, in a rat tibial defect model, this treatment strongly promoted bone regeneration, increasing the bone volume fraction (BV/TV), bone volume (BV), and trabecular thickness (Tb.Th) at 4 weeks post-surgery. Mechanistically, proteomic analysis revealed that NAMPT in 3D-sEVs upregulated S1PR1 in HUVECs, leading to increased VEGF expression and angiogenesis. These findings suggest that 3D-sEVs-functionalized HAMA-CPC hydrogels have good potential for bone defect repair.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"531"},"PeriodicalIF":10.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12278526/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An adeno-associated virus gene therapy strategy for anti-obesity treatment by nanocarrier-based delivery systems. 基于纳米载体的递送系统抗肥胖的腺相关病毒基因治疗策略。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2025-07-21 DOI: 10.1186/s12951-025-03595-5

Li Li, Wang Wang, Rong Li, Jiayue Guo, Xulin Hu, Yu Pan, Taoyuan Zhang, Sensen Chi, Zili Gu, Gaohui Zhu, Qi Liu, Shuai Tan

{"title":"An adeno-associated virus gene therapy strategy for anti-obesity treatment by nanocarrier-based delivery systems.","authors":"Li Li, Wang Wang, Rong Li, Jiayue Guo, Xulin Hu, Yu Pan, Taoyuan Zhang, Sensen Chi, Zili Gu, Gaohui Zhu, Qi Liu, Shuai Tan","doi":"10.1186/s12951-025-03595-5","DOIUrl":"10.1186/s12951-025-03595-5","url":null,"abstract":"Gut microbiota is increasingly recognized for its profound influence on host metabolism. However, the mechanisms underlying the distinct metabolic phenotype observed in germ-free (GF) mice are not fully understood. Here, the serum levels of metabolic hormones glucagon-like peptide-1 (GLP-1) and peptide YY (PYY) were shown with significant elevation in GF than the conventionally-raised (CONV-R) mice. Single-cell transcriptome analysis revealed that Secretogranin II (Scg2), with a known function in secretion control, was exclusively expressed in enteroendocrine cells (EECs). Scg2 transcript levels were significantly up-regulated in GF mice, positively correlated with enhanced GLP-1 and peptide PYY secretion. To examine the functional significance of Scg2 in hormone regulation, cross-linked nanoparticles capable of long-term adhesion to intestinal epithelium were designed, with AAV adsorbed within the cross-linked structure. This innovative design enhances its stability and retention in vivo, providing a robust platform for continuous and efficient gene delivery. We evidenced that over-expression of Scg2 via AAV-loaded nanocarriers in the colons of mice on high-fat diet or with the ob/ob genotype not only enhanced the secretion of GLP-1 and PYY but also mitigated weight gain in these mice by reducing their appetite. A multi-omics analysis reveals that Scg2 overexpression in the colon decreased hypothalamic inflammation and activated tryptophan metabolic pathways. Collectively, our findings suggest a potential therapeutic approach for treating metabolic disorders by enhancing Scg2 expression in colonic EECs.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"528"},"PeriodicalIF":10.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12278491/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing exosome stability and delivery with natural polymers to prevent intrauterine adhesions and promote endometrial regeneration: a review. 用天然聚合物增强外泌体稳定性和递送以防止宫内粘连和促进子宫内膜再生：综述

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2025-07-21 DOI: 10.1186/s12951-025-03603-8

Wei Sun, Wei Xu, Miaomiao Xiao, Xinge Zhang, Jing Chen, Jinzhe Zhang, Liqun Yang, Quan Na

{"title":"Enhancing exosome stability and delivery with natural polymers to prevent intrauterine adhesions and promote endometrial regeneration: a review.","authors":"Wei Sun, Wei Xu, Miaomiao Xiao, Xinge Zhang, Jing Chen, Jinzhe Zhang, Liqun Yang, Quan Na","doi":"10.1186/s12951-025-03603-8","DOIUrl":"10.1186/s12951-025-03603-8","url":null,"abstract":"Intrauterine adhesions (IUAs) are defined as the formation of fibrotic scar tissue within the uterine cavity. They can lead to a range of complications, including hypomenorrhea, amenorrhea, infertility, and recurrent pregnancy loss. Conventional methods of adhesiolysis frequently demonstrate high recurrence rates and suboptimal therapeutic outcomes. Moreover, conventional postoperative IUA prevention strategies (e.g., physical barriers and hormonal therapy) are ineffective and have adverse effects, emphasizing the need for new therapies. Natural polymers are ideal biomaterials for barrier membranes and drug delivery in uterine repair due to their safety, biodegradability, and compatibility. Exosomes, tiny cell-released vesicles containing healing factors (like miRNAs and proteins), help reduce inflammation and scarring while promoting tissue regeneration. Emerging evidence suggests that combining natural polymers with exosomes could provide a potential therapeutic approach for IUAs and endometrial regeneration. This review presents a comprehensive overview of the biological characteristics of natural polymers and exosomes, elucidating their mechanisms of action in endometrial repair. It also provides an in-depth analysis of the current research landscape pertaining to the combined delivery of natural polymers and exosomes in the prevention of IUAs and the promotion of endometrial regeneration.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"529"},"PeriodicalIF":10.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12278673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prunus mume derived extracellular vesicle-like particles alleviate experimental colitis via disrupting NEK7-NLRP3 interaction and inflammasome activation. 梅子衍生的细胞外囊泡样颗粒通过破坏NEK7-NLRP3相互作用和炎症小体激活来减轻实验性结肠炎。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2025-07-21 DOI: 10.1186/s12951-025-03567-9

Qi Lv, Hongqiong Yang, Ying Xie, Xinjie Huang, Zhiqi Yan, Yingshan Lv, Yifan Cui, Lihong Hu, Hongzhi Qiao

{"title":"Prunus mume derived extracellular vesicle-like particles alleviate experimental colitis via disrupting NEK7-NLRP3 interaction and inflammasome activation.","authors":"Qi Lv, Hongqiong Yang, Ying Xie, Xinjie Huang, Zhiqi Yan, Yingshan Lv, Yifan Cui, Lihong Hu, Hongzhi Qiao","doi":"10.1186/s12951-025-03567-9","DOIUrl":"10.1186/s12951-025-03567-9","url":null,"abstract":"Edible plant derived extracellular vesicle-like particles (EVLPs) have garnered attention as potential therapeutic agents for chronic inflammatory diseases. Prunus mume (PM) is a functional fruit known for its gastrointestinal benefits, yet the detail material basis and potential mechanism remain unclear. Here, we reported that oral administration of prunus mume derived EVLPs (PM-EVLPs) substantially mitigated experimental colitis in mice. The in vivo bio-distribution analysis revealed that PM-EVLPs specifically targeted inflamed colon of colitis mice. Further in vitro studies demonstrated that PM-EVLPs were predominantly internalized by macrophages. The combined treatment with clodronate liposomes confirmed that macrophage was the target cell for PM-EVLPs-mediated anti-colitis activity. Mechanistically, PM-EVLPs selectively inhibited caspase-1 auto-cleavage and IL-1β secretion caused by NLRP3 inflammasome activation, while exerting minimal impact on AIM2, NLRP1 or NLRC4 inflammasome activation. Excluding the effects on mitochondrial ROS generation, K+ efflux or Ca2+ influx, PM-EVLPs disrupted the NEK7-NLRP3 interaction, thereby preventing NLRP3 inflammasome assembly. Notably, the inhibitory activity was attributed to RNAs rather than lipids or proteins within PM-EVLPs. Deep RNA sequencing, coupled with the application of miRNA mimics/inhibitors identified miR159 as the material basis for PM-EVLPs' inhibition of NLRP3 inflammasome activation and anti-colitis efficacy. Collectively, these findings suggest that PM-EVLPs represent a promising nanomedicine with potential as a novel therapeutic strategy for colitis and deserves further investigation and development.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"532"},"PeriodicalIF":10.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12278495/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0