Journal of Nanobiotechnology最新文献

Dual-source powered sea urchin-like nanomotors for intravesical photothermal therapy of bladder cancer. 双源动力海胆样纳米马达用于膀胱内光热治疗膀胱癌。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2025-05-17 DOI: 10.1186/s12951-025-03446-3

Yixuan Mou, Zhenghong Liu, Wentao Xu, Bin Zheng, Minghai Ma, Xiaowen Qin, Jiajia Zheng, Ran Ni, Haichang Li, Lei Wang, Yuchen Bai, Jinhai Fan, Xiaolong Qi, Qi Zhang, Pu Zhang, Dahong Zhang

{"title":"Dual-source powered sea urchin-like nanomotors for intravesical photothermal therapy of bladder cancer.","authors":"Yixuan Mou, Zhenghong Liu, Wentao Xu, Bin Zheng, Minghai Ma, Xiaowen Qin, Jiajia Zheng, Ran Ni, Haichang Li, Lei Wang, Yuchen Bai, Jinhai Fan, Xiaolong Qi, Qi Zhang, Pu Zhang, Dahong Zhang","doi":"10.1186/s12951-025-03446-3","DOIUrl":"https://doi.org/10.1186/s12951-025-03446-3","url":null,"abstract":"Bladder cancer (BCa) ranks as the 9th most prevalent malignancy worldwide, featured by its high risk of recurrence. Intravesical therapy constitutes the most important modality to tackle BCa, but its efficiency is often compromised due to the dense physiological barriers in BCa, the instability of the catalytic environment, and the rapid clearance facilitated by periodic urination. Here, we present a dual-source powered sea urchin-like nanomotor, which feature a gold nanocore decorated with ultrasmall platinum nanoparticles and ureases, enable rapid propulsion through the catalytic conversion of abundant urea and hydrogen peroxide present in the bladder cavity and BCa microenvironment, respectively. Our dual-source powered Au-Pt@ur NPs nanoparticles translocated across the mucus barrier rapidly, deeply penetrated tumor and hence chemo-resected bladder tumors in all cases. These results hold substantial promise for the development of biocompatible nanomotors for improved BCa intravesical therapy.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"355"},"PeriodicalIF":10.6,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144086246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NIR-responsive Cu_2 - xSe@Fc nanoparticles for photothermal- ferroptosis combination therapy in esophageal cancer. nir响应Cu2 - xSe@Fc纳米颗粒光热-铁下垂联合治疗食管癌。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2025-05-17 DOI: 10.1186/s12951-025-03434-7

Linlin Shi, Ying Yu, Jiayi Li, Beng Ma, Xiaoman Zhang, Pingjuan Yang, Pan Chen, Zhifeng Qu, Fengqi Zhang, Ke Liu, Shegan Gao, Haoyan Cheng

{"title":"NIR-responsive Cu2 - xSe@Fc nanoparticles for photothermal- ferroptosis combination therapy in esophageal cancer.","authors":"Linlin Shi, Ying Yu, Jiayi Li, Beng Ma, Xiaoman Zhang, Pingjuan Yang, Pan Chen, Zhifeng Qu, Fengqi Zhang, Ke Liu, Shegan Gao, Haoyan Cheng","doi":"10.1186/s12951-025-03434-7","DOIUrl":"https://doi.org/10.1186/s12951-025-03434-7","url":null,"abstract":"Esophageal cancer (EC) represents a highly recurrent and aggressive malignancy within the digestive system. However, conventional therapeutic strategies exhibit notable limitations in their clinical applications. Photothermal therapy (PTT), combined with ferroptosis, has attracted considerable attentions, emerging as a promising novel strategy for EC treatment. Therefore, there is a critical need to develop a drug delivery system capable of effectively integrating these two therapeutic approaches. In this work, we report a novel drug delivery system based on ferrocene (Fc), which is mixed with lauric acid (a phase-change material with a melting point around 44 oC) and then coated on the surface of Cu2 - xSe nanoparticles. The photothermal properties of Cu2 - xSe triggers the melting of lauric acid under near-infrared (NIR) laser irradiation, facilitating controlled release of Fc. Following internalization by tumor cells via endocytosis, the synergistic effect of PTT and ferroptosis, triggered by Cu2 - xSe@Fc, induced immunogenic cell death, which promoted dendritic cell maturation and cytotoxic T lymphocytes recruitment while decreasing the proportion of regulatory T cells, thereby strengthening the antitumor immune surveillance and improving the therapeutic efficacy of Anti-PD-1 blockade. These findings propose that the NIR-responsive Cu2 - xSe@Fc formulation represents a promising and effective strategy with prospecting application for cancer treatment.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"356"},"PeriodicalIF":10.6,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144086251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

M2-ApoBDs as a therapeutic strategy for systemic lupus erythematosus: targeted macrophage reprogramming and treg differentiation. M2-ApoBDs作为系统性红斑狼疮的治疗策略：靶向巨噬细胞重编程和treg分化。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2025-05-16 DOI: 10.1186/s12951-025-03437-4

Juan Ji, Shaoying Yang, Yongxin Xu, Qian He, Qian Liang, Guijuan Feng, Yunfei Xia, Mei Yang, Yuting Huang, Junling Yang, Chen Dong, Rui Zhao, Yunan Wang, Genkai Guo, Xiaoqi Sha, Jing Li, Yuehua Guo, Zhifeng Gu

{"title":"M2-ApoBDs as a therapeutic strategy for systemic lupus erythematosus: targeted macrophage reprogramming and treg differentiation.","authors":"Juan Ji, Shaoying Yang, Yongxin Xu, Qian He, Qian Liang, Guijuan Feng, Yunfei Xia, Mei Yang, Yuting Huang, Junling Yang, Chen Dong, Rui Zhao, Yunan Wang, Genkai Guo, Xiaoqi Sha, Jing Li, Yuehua Guo, Zhifeng Gu","doi":"10.1186/s12951-025-03437-4","DOIUrl":"https://doi.org/10.1186/s12951-025-03437-4","url":null,"abstract":"Systemic lupus erythematosus (SLE) is a chronic autoimmune disorder that affects various organs and systems, significantly impacting patients' health and quality of life. Conventional drugs, including glucocorticoids and standard immunosuppressive drugs, may not be enough to achieve a satisfactory therapeutic outcome in some refractory SLE patients. The abnormal phenotype and function of macrophages participate in the development of SLE. The targeted delivery to reprogram macrophage in SLE has been a long-standing challenge. Apoptotic bodies (ApoBDs) are essential for intercellular communications. This study aims to explore an effective and targeted treatment to SLE via macrophage reprogramming and Treg differentiation. In this work, we found that M2 macrophages-derived ApoBDs (M2-ApoBDs) could selectively target and localize to the spleen, where they were engulfed by splenic macrophages (phagocytic rate 73.4%). Single-cell RNA sequencing revealed that the efferocytosis of M2-ApoBDs triggered transcriptional changes in M2 (anti-inflammatory) macrophages within the spleen, subsequently promoting the differentiation of Treg cells in vivo. Immunological experiments revealed that M2-ApoBDs prompted the reprogramming of M2 macrophages in vitro, which subsequently influenced Treg cell differentiation via ligand-receptor interactions. In SLE mice, M2-ApoBDs alleviated the disease progression, including 24-hours urinary protein, plasma creatinine, plasma C3 levels, and glomerular sclerosis and interstitial fibrosis. These findings show that M2-ApoBDs can targeted-modulate macrophage polarization and Treg immune regulation, offering a novel therapeutic strategy for the effective treatment of SLE.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"354"},"PeriodicalIF":10.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144086249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxygen vacancy-engineered bimetallic nanozymes for disrupting electron transport chain and synergistic multi-enzyme activity to reverse oxaliplatin resistance in colorectal cancer. 氧空位工程双金属纳米酶用于破坏电子传递链和协同多酶活性以逆转结直肠癌的奥沙利铂耐药。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2025-05-16 DOI: 10.1186/s12951-025-03417-8

Dong Zhong, Xiaoxin Yang, Jinhui Yang, Zhisheng Luo, Zhichao Feng, Mengtian Ma, Yunjie Liao, Yongxiang Tang, Yu Wen, Jun Liu, Shuo Hu

{"title":"Oxygen vacancy-engineered bimetallic nanozymes for disrupting electron transport chain and synergistic multi-enzyme activity to reverse oxaliplatin resistance in colorectal cancer.","authors":"Dong Zhong, Xiaoxin Yang, Jinhui Yang, Zhisheng Luo, Zhichao Feng, Mengtian Ma, Yunjie Liao, Yongxiang Tang, Yu Wen, Jun Liu, Shuo Hu","doi":"10.1186/s12951-025-03417-8","DOIUrl":"https://doi.org/10.1186/s12951-025-03417-8","url":null,"abstract":"In colorectal cancer treatment, chemotherapeutic agents induce reactive oxygen species (ROS) production, which promotes NAD+ accumulation in tumor cells, reducing treatment sensitivity and worsening patient prognosis. Targeted depletion of NAD+ presents a promising strategy to overcome tumor resistance and improve patient prognosis. Here, we designed a dual-metallic nanozyme (CuMnOx-V@Oxa@SP) with defect engineering, modified by soy phospholipids (SP) and loaded with oxaliplatin (Oxa). This nanozyme uses its oxygen-deficient active sites to rapidly and irreversibly degrade NAD⁺ and NADH into nicotinamide and ADP-ribose derivatives, disrupting the electron transport chain (ETC) and compromising tumor antioxidant defenses. It also inhibits the glutathione S-transferase P1 (GSTP1) pathway, weakening tumor detoxification and enhancing chemotherapy sensitivity. Density functional theory calculations revealed that the synergistic effect among multi-enzyme active centers endows the CuMnOx-V nanozymes with excellent catalytic activity. In the tumor microenvironment (TME), CuMnOx-V nanozymes exhibit peroxidase, oxidase, and NAD+ oxidase-mimicking activities. CuMnOx-V generates multiple ROS and depletes NAD+ while preventing their regeneration thereby triggering a cascade amplification of oxidative stress. This, coupled with targeted chemotherapy drug delivery, restores chemosensitivity in refractory tumors and exposes the vulnerabilities of resistant colorectal cancer cells to ROS.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"352"},"PeriodicalIF":10.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144086252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The biological activity and potential of probiotics-derived extracellular vesicles as postbiotics in modulating microbiota-host communication. 益生菌衍生的细胞外囊泡作为后生制剂在调节微生物-宿主通讯中的生物活性和潜力。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2025-05-16 DOI: 10.1186/s12951-025-03435-6

Xiaoming Zhang, Ye Wang, Qiyu E, Muhammad Naveed, Xiuli Wang, Yinhui Liu, Ming Li

{"title":"The biological activity and potential of probiotics-derived extracellular vesicles as postbiotics in modulating microbiota-host communication.","authors":"Xiaoming Zhang, Ye Wang, Qiyu E, Muhammad Naveed, Xiuli Wang, Yinhui Liu, Ming Li","doi":"10.1186/s12951-025-03435-6","DOIUrl":"https://doi.org/10.1186/s12951-025-03435-6","url":null,"abstract":"Probiotics such as Lactobacillus and Bifidobacterium spp. have been shown to be critical for maintaining host homeostasis. In recent years, key compounds of postbiotics derived from probiotic metabolism and cellular secretion have been identified for their role in maintaining organ immunity and regulating intestinal inflammation. In particular, probiotic-derived extracellular vesicles (PEVs) can act as postbiotics, maintaining almost the same functional activity as probiotics. They also have strong biocompatibility and loading capacity to carry exogenous or parental active molecules to reach distal organs to play their roles. This provides a new direction for understanding the intrinsic microbiota-host communication mechanism. However, most current studies on PEVs are limited to their functional effects/benefits, and their specific physicochemical properties, composition, intrinsic mechanisms for maintaining host homeostasis, and possible threats remain to be explored. Here, we review and summarize the unique physicochemical properties of PEVs and their bioactivities and mechanisms in mediating microbiota-host communication, and elucidate the limitations of the current research on PEVs and their potential application as postbiotics.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"349"},"PeriodicalIF":10.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144086253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced therapeutic effects of all-trans retinoic acid nanostructured lipid carrier composite gel drug delivery system for alopecia areata. 全反式维甲酸纳米结构脂质载体复合凝胶给药系统对斑秃的治疗效果增强。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2025-05-16 DOI: 10.1186/s12951-025-03407-w

Lingling Zheng, Yang Du, Lulu Zhang, Fuxing Jin, Wangting Li, Xuan Zhou, Yanping Yin, Yan Weng, Dong Xu, Jingwen Wang

{"title":"Enhanced therapeutic effects of all-trans retinoic acid nanostructured lipid carrier composite gel drug delivery system for alopecia areata.","authors":"Lingling Zheng, Yang Du, Lulu Zhang, Fuxing Jin, Wangting Li, Xuan Zhou, Yanping Yin, Yan Weng, Dong Xu, Jingwen Wang","doi":"10.1186/s12951-025-03407-w","DOIUrl":"https://doi.org/10.1186/s12951-025-03407-w","url":null,"abstract":"Background: Alopecia areata (AA) affects approximately 2% of the global population and causes psychological distress. All-trans retinoic acid (ATRA) has the potential to promote hair regeneration; however, its clinical use is limited by skin irritation and low targeting specificity. To address these limitations, we designed an ATRA-loaded nanostructured lipid carrier gel (ATRA-NLC-Gel) drug delivery system to enhance the therapeutic effects of ATRA in AA.Results: ATRA-NLC showed a uniform nanoparticle size distribution and excellent biocompatibility. In vitro, they enhanced the uptake ability of dermal papilla cells, increased cell viability, and promoted cell proliferation by facilitating the cell cycle process. Compared to ATRA cream, ATRA-NLC-Gel significantly reduced skin irritation, prolonged residence time on the skin, and achieved a sustained and slow release of ATRA. Treatment with ATRA-NLC-Gel enhanced transdermal penetration and targeted enrichment in the hair follicle region, thereby significantly promoting hair regrowth. ATRA-NLC-Gel improved AA symptoms by upregulating CD200 and Ki-67 expression, activating the Wnt/β-catenin pathway.Conclusions: ATRA-NLC-Gel enhanced the transdermal permeability and follicle-targeting efficacy of ATRA, alleviated ATRA-induced skin dryness and irritation, and effectively improved the symptoms of AA in AA model mice. ATRA-NLC-Gel offers a highly promising strategy for transdermal treatment of AA in clinical setting.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"351"},"PeriodicalIF":10.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144086248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in microneedle-based drug delivery system for metabolic diseases: structural considerations, design strategies, and future perspectives. 代谢疾病微针给药系统的研究进展：结构考虑、设计策略和未来展望。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2025-05-16 DOI: 10.1186/s12951-025-03432-9

Yao Li, Qiu Chen, Tingting Wang, Zengkai Ji, Sagar Regmi, Haibin Tong, Jian Ju, Aifang Wang

{"title":"Advances in microneedle-based drug delivery system for metabolic diseases: structural considerations, design strategies, and future perspectives.","authors":"Yao Li, Qiu Chen, Tingting Wang, Zengkai Ji, Sagar Regmi, Haibin Tong, Jian Ju, Aifang Wang","doi":"10.1186/s12951-025-03432-9","DOIUrl":"https://doi.org/10.1186/s12951-025-03432-9","url":null,"abstract":"As the prevalence of metabolic diseases such as diabetes and obesity continue to rise, the search for more effective and convenient treatments has become a crucial issue in medical research. Microneedles (MNs), as an innovative drug delivery system, have shown advantages in the treatment of metabolic diseases in recent years. MNs-based drug delivery system, which use MNs to deliver drugs directly to the subcutaneous tissue, improve drug bioavailability and reduce systemic side effects. This review aims to summarize the latest concepts, designs, and types of MNs, and to investigate the materials and manufacturing methods used in their construction. Subsequently, the mechanisms of drug delivery and graded release of MNs and recent research progress are further summarized. This article focuses on the application of MNs in the treatment of common metabolic diseases, with a special emphasis on the progress and optimization of diabetic and anti-obesity MNs. The main challenges and future perspectives in the production and evaluation of MNs, as well as in enhancing treatment efficacy and improving safety, are elucidated.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"350"},"PeriodicalIF":10.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144086245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A spreadable self-gelling hemostatic powder sensitizes CAR-NK cell therapy to prevent hepatocellular carcinoma recurrence postresection. 一种可涂抹的自凝胶止血粉使CAR-NK细胞治疗增敏，以预防肝癌切除术后复发。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2025-05-16 DOI: 10.1186/s12951-025-03424-9

Yusheng Cheng, Yihang Gong, Xin Li, Fanxin Zeng, Bo Liu, Wenjie Chen, Feng Zhang, Haofei Chen, Weixiong Zhu, Hui Li, Lei Zhou, Tiangen Wu, Wence Zhou

{"title":"A spreadable self-gelling hemostatic powder sensitizes CAR-NK cell therapy to prevent hepatocellular carcinoma recurrence postresection.","authors":"Yusheng Cheng, Yihang Gong, Xin Li, Fanxin Zeng, Bo Liu, Wenjie Chen, Feng Zhang, Haofei Chen, Weixiong Zhu, Hui Li, Lei Zhou, Tiangen Wu, Wence Zhou","doi":"10.1186/s12951-025-03424-9","DOIUrl":"https://doi.org/10.1186/s12951-025-03424-9","url":null,"abstract":"Adoptive natural killer cell therapy (ANKCT) harbors great potential for combating postsurgical hepatocellular carcinoma (HCC) recurrence, but its efficacy is limited by tumor microenvironment (TME)-meditated repression on NK cell function and insufficient NK cell homing to tumor sites. Therefore, herein we develop a nanocomposite sprayable self-gelling powder enabling liver-localized codelivery of three FDA-approved drugs including calcitriol (Cal), gemcitabine (Gem), and tazemetostat (Taz) to address these challenges. This powder can be laparoscopically spread to liver wound sites, where it rapidly absorbs interfacial liquid to form a bulk adhesive pressure-resistant hydrogel in situ, implying its application potential in minimally surgery. Moreover, its application to liver resection bed significantly sensitizes allogenic NK and EpCAM chimeric antigen receptor modified-NK-92 (EpCAM-CAR-NK) cell infusion to prevent HCC recurrence in orthotopic Heap1-6 tumor-bearing and patient-derived tumor xenograft (PDX) HCC murine models. Additionally, this powder can allow for an effective hemostatic effect in rat and porcine models due to its powerful tissue adhesion-seal and erythrocyte-aggregating effects. Altogether, our newly developed hemostatic self-gelling powder can significantly sensitize ANKCT to combat HCC recurrence in a manner compatible with surgical treatment of HCC.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"353"},"PeriodicalIF":10.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144086244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synphilin-1 regulates mechanotransduction in rigidity sensing through interaction with zyxin. Synphilin-1通过与zyxin的相互作用调节刚性感应的机械转导。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2025-05-14 DOI: 10.1186/s12951-025-03429-4

Seok Gi Kim, Jinyan Li, Ji Su Hwang, Muhammad Anwar Ul Hassan, Ye Eun Sim, Ju Yeon Lee, Jung-Soon Mo, Myeong Ok Kim, Gwang Lee, Sungsu Park

{"title":"Synphilin-1 regulates mechanotransduction in rigidity sensing through interaction with zyxin.","authors":"Seok Gi Kim, Jinyan Li, Ji Su Hwang, Muhammad Anwar Ul Hassan, Ye Eun Sim, Ju Yeon Lee, Jung-Soon Mo, Myeong Ok Kim, Gwang Lee, Sungsu Park","doi":"10.1186/s12951-025-03429-4","DOIUrl":"https://doi.org/10.1186/s12951-025-03429-4","url":null,"abstract":"Background: Synphilin-1 has been studied extensively in the context of Parkinson's disease pathology. However, the biophysical functions of synphilin-1 remain unexplored. To investigate its novel functionalities herein, cellular traction force and rigidity sensing ability are analyzed based on synphilin-1 overexpression using elastomeric pillar arrays and substrates of varying stiffness. Molecular changes are analyzed using RNA sequencing-based transcriptomic and liquid chromatography-tandem mass spectrometry-based proteomic analyses.Results: Synphilin-1 overexpression reduces cell area, with a decline of local contraction on elastomeric pillar arrays. Cells overexpressing synphilin-1 exhibit an impaired ability to respond to substrate rigidity; however, synphilin-1 knockdown restores rigidity sensing abilities. Integrated omics analysis and in silico prediction corroborate the phenotypic alterations induced by synphilin-1 overexpression at a biophysical level. Zyxin emerges as a novel synphilin-1 binding protein, and synphilin-1 overexpression reduces the nuclear translocation of yes-associated protein.Conclusion: These findings provide novel insights into the biophysical functions of synphilin-1, suggesting a potential protective role to the altered extracellular matrix, which may be relevant to neurodegenerative conditions such as Parkinson's disease.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"345"},"PeriodicalIF":10.6,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12076907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PEGylated gas vesicles: a promising novel ultrasound contrast agent for diagnosis and guiding radiofrequency ablation of liver tumor. 聚乙二醇化气体囊泡：一种有前途的新型超声造影剂，用于诊断和指导肝脏肿瘤的射频消融。

IF 10.6 1区生物学

Journal of Nanobiotechnology Pub Date : 2025-05-14 DOI: 10.1186/s12951-025-03377-z

Kezhi Yu, Yongquan Huang, Yuanyuan Wang, Qunyan Wu, Zihang Wang, Fei Li, Jianri Chen, Maierhaba Yibulayin, Shushan Zhang, Zhongzhen Su, Fei Yan

{"title":"PEGylated gas vesicles: a promising novel ultrasound contrast agent for diagnosis and guiding radiofrequency ablation of liver tumor.","authors":"Kezhi Yu, Yongquan Huang, Yuanyuan Wang, Qunyan Wu, Zihang Wang, Fei Li, Jianri Chen, Maierhaba Yibulayin, Shushan Zhang, Zhongzhen Su, Fei Yan","doi":"10.1186/s12951-025-03377-z","DOIUrl":"https://doi.org/10.1186/s12951-025-03377-z","url":null,"abstract":"Ultrasound contrast agents (UCAs) play an important role in diagnosis and the imaging-guided treatment of liver tumor in clinical settings. However, most commercially available UCAs are micro-sized and fabricated through a chemical synthesis route. Here, we developed a new class of biosynthesized nanoscale contrast agent (PEG-GVs) and comprehensively compared its physicochemical characteristics and imaging performance with commercial Sonovue and Sonazoid. Our results revealed PEG-GVs may produce more stable and durable contrast signals, contributing to their penetration beyond blood vessels and long-time retention in liver. Interestingly, we found that PEG-GVs did not exhibit a continuously enhanced accumulation in the liver tumor due to the EPR effect, but displayed a rapid regression. The long-time retention of PEG-GVs in normal liver tissue and rapid regression from liver tumor lead to distinct display of liver tumor boundaries, enabling the early diagnosis of small liver metastases and presenting advantages in guiding radiofrequency ablation of liver tumor. Moreover, we have also verified that PEG-GVs exhibit excellent imaging performance and biosafety in macaques. Our study provides new insights into the roles of PEG-GVs in liver tumor diagnosis and ablation guidance.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"344"},"PeriodicalIF":10.6,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12076873/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0