Journal of Nanobiotechnology最新文献

{"title":"Mechanically tunable fiber-based hydrogel activates PIEZO1-integrin axis for enhanced bone repair.","authors":"Jinghong Yang, Runli Li, Xiaoshuang Wang, Dongheng Lu, Weichang Li, Yan Wang","doi":"10.1186/s12951-025-03653-y","DOIUrl":"10.1186/s12951-025-03653-y","url":null,"abstract":"<p><p>Irregular alveolar bone defects pose persistent clinical challenges due to their complex morphology and the lack of biomaterials that simultaneously provide structural integrity, biocompatibility, and dynamic osteoinductive potential. Herein, we report a fiber-reinforced, dual-network hydrogel system (OHADN fiber@Yoda1 hydrogel) engineered to recapitulate mechanobiological cues for enhanced bone regeneration. This injectable hydrogel integrates oxidized hyaluronic acid (OHA) crosslinked with Yoda1-loaded PLGA-collagen fiber fragments and stabilized via catechol-Fe³⁺ coordination, forming a robust and self-healing structure. The fiber network enhances matrix stiffness and sustains Yoda1 release, promoting PIEZO1 activation in stem cells and enabling persistent mechanotransduction. In vitro, this system effectively regulates macrophage polarization, maintains cellular tension homeostasis, and significantly upregulates osteogenic markers via the PIEZO1-ITGα5 axis. Transcriptomic profiling and mechanistic validation revealed that focal adhesion and cytoskeletal signaling pathways are enriched upon hydrogel treatment. In a rat alveolar bone defect model, the OHADN fiber@Yoda1 hydrogel demonstrated superior bone volume restoration and trabecular architecture compared to conventional materials. This work presents a promising paradigm for spatiotemporal control of osteoimmune microenvironments through mechanoresponsive biomaterials.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"603"},"PeriodicalIF":12.6,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12406391/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144992873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Separable cryo-microneedle patches delivery with capsaicin integrated mesoporous dopamine for obesity treatment.","authors":"Jingjing Gan, Lingyu Sun, Wenjuan Tang, Yuanjin Zhao, Yan Bi","doi":"10.1186/s12951-025-03645-y","DOIUrl":"10.1186/s12951-025-03645-y","url":null,"abstract":"<p><p>Microneedle patch encapsulated with active medication holds significant potential promise in the realm of anti-obesity therapy. Nevertheless, the improvement of actives delivery efficiency remains a significant challenge. In this paper, we present novel separable cryo-microneedles patches delivered with capsaicin integrated mesoporous dopamine (mPDA) for obesity treatment through activating TRPV1 and inducing lipid droplet dissolution. Such patch is fabricated by cryogenic molding of biocompatible hydrogel tips, which can be easily inserted and left from skin through the thermoresponsive backing layer. Upon penetration, these tips could release mPDA-carrying capsaicin into the targeted subcutaneous adipose layer. In addition, when exposed to near infrared (NIR) light, these mPDA could rapidly convert the light energy into heat to increase the local temperature, thus accelerating the release of capsaicin from mPDA and the metabolic benefits of regional hyperthermia therapy. Based on these features, we have demonstrated that the multifunctional separated cryo-microneedles could significantly reduce weight and relieve obesity-associated pathological conditions with no adverse effect through an 8-week observation on high-fat diet (HFD) mice. Thus, we believe that our separable cryo-microneedles patches delivered with capsaicin integrated mPDA will open a new prospect for obesity treatment.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"604"},"PeriodicalIF":12.6,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12406480/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144992790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoscale metal-organic frameworks as a versatile platform for synergistic combination tumor therapy.","authors":"Mengru Cai, Yuqing Yang, Jiahui Kong, Yongqiang Zhang, Shiman Li, Huizhong Zhang, Xiaohan Xv, Jian Ni, Xingbin Yin","doi":"10.1186/s12951-025-03625-2","DOIUrl":"10.1186/s12951-025-03625-2","url":null,"abstract":"<p><p>As the second most common cause of death globally, tumor significantly impacts human health and quality of life. Although monotherapy has achieved remarkable progress, its therapeutic effectiveness remains less than ideal. The current strategy is increasingly inclined towards a combination of different treatments. Combination therapy is conducive to rectifying the inherent flaws of monotherapy, surmounting multi-drug resistance, and attaining a synergistic effect. The advancement of nanotechnology offers an unparalleled opportunity for anti-tumor combination therapy. Metal-organic frameworks (MOFs) possess distinctive structural features, including adjustable pore sizes, diverse organic ligands and metal ion centers, as well as the modifiability of functional sites. These characteristics render the combined application of multiple therapeutic approaches feasible. This article provides a review of the research on MOFs-based nano drug delivery platform for anti-tumor combination therapy under physiological conditions or exogenous stimulation. The opportunities and challenges of MOFs entering the clinic for anti-tumor combination therapy are also discussed to provide reference for the further application of MOFs, and provide a novel idea for the research of MOFs-based drug delivery system.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"601"},"PeriodicalIF":12.6,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12403579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spleen-targeted NeoPol-mL242 mRNA vaccine induces robust T-cell responses in a hepatocellular carcinoma model.","authors":"Yufei Wu, Gongrui Sun, Wendan Ren, Yang Gui, Cong Wang, Xinyi Ye, Yun Chen, Xiufeng Pang, Qi Zhang, Zi Jun Wang, Yuxuan Wu","doi":"10.1186/s12951-025-03681-8","DOIUrl":"10.1186/s12951-025-03681-8","url":null,"abstract":"<p><p>Personalized neoantigen peptide vaccines have shown remarkable anti-tumor activity across diverse cancer types. With the rapid advancement of messenger RNA (mRNA) delivery technologies during the coronavirus disease of 2019 (COVID-19) pandemic, mRNA-based cancer vaccines have emerged as a promising therapeutic approach because of their scalable production, safety, and capacity to elicit potent immune responses. However, the predominant distribution of mRNA delivery systems in the liver may lead to hepatic damage and restrict therapeutic accessibility. In this study, we designed a novel ionizable lipid library to shift the delivery to the spleen. By incorporating an additional anionic lipid, we identified an optimized vaccine formulation, which exhibited efficient uptake by dendritic cells (DCs). Notably, this formulation achieved spleen-selective delivery without requiring targeting ligand modifications, thereby minimizing cytotoxicity risks. Furthermore, the spleen-targeted L242-20Lipo nanoparticle was employed to facilitate the efficient delivery of personalized neoantigen mRNA vaccines. Evaluation in a hepatocellular carcinoma (HCC) model demonstrated that the NeoPol-mL242 mRNA vaccine elicited potent anti-tumor immunity while maintaining an excellent safety profile. These results highlight NeoPol-mL242 as a promising candidate for application in cancer immunotherapy.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"602"},"PeriodicalIF":12.6,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12403396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circadian disruption and ROS-NLRP3 signaling mediate sleep deprivation-enhanced silica nanoparticle toxicity in lacrimal glands.","authors":"Wenxiao Zhang, Di Qi, Xiaoting Pei, Dingli Lu, Mengru Ba, Shuting Xuan, Duliurui Huang, Tingting Yang, Jingwen Yang, Zhijie Li, Shenzhen Huang","doi":"10.1186/s12951-025-03630-5","DOIUrl":"10.1186/s12951-025-03630-5","url":null,"abstract":"<p><p>Sleep deprivation (SD) and exposure to engineered nanomaterials such as silica nanoparticles (SiNPs) are emerging risk factors for ocular surface disorders, particularly dry eye disease. However, the molecular mechanisms underlying their combined impact on lacrimal gland function remain unclear. In this study, we investigated the synergistic effects of SD and SiNPs exposure on circadian regulation, oxidative stress, inflammation, and structural integrity of the extraorbital lacrimal glands (ELGs) in C57BL/6J mice. Behavioral and physiological monitoring revealed that SD + SiNPs disrupted circadian locomotor activity and body temperature rhythms. Phenotypic assessments showed reduced tear secretion and ELG atrophy. RNA sequencing identified extensive transcriptomic reprogramming, including altered expression of core clock genes and enrichment of inflammatory and redox-related pathways. Increased reactive oxygen species (ROS) accumulation and γ-H2AX expression indicated oxidative DNA damage. Immunohistochemistry confirmed NLRP3 inflammasome activation, while Western blotting revealed enhanced phosphorylation of JAK2, STAT3, NF-κB p65, and IκBα, alongside upregulation of IL-17A. Elevated malondialdehyde levels further reflected oxidative lipid damage. These findings demonstrate that SD exacerbates SiNPs-induced ELG dysfunction via circadian disruption and activation of the ROS/NLRP3/IL-17A inflammatory axis. While these effects are currently limited to the lacrimal gland, future studies are needed to determine whether similar mechanisms contribute to broader systemic metabolic consequences.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"600"},"PeriodicalIF":12.6,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12403269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dectin-1-targeted pH-responsive liposomal nanoplatform delivering Plantago Asiatica L. acidic polysaccharide for immunomodulation and immunosuppressive breast cancer microenvironment reprogramming.","authors":"Wanbing Pan, Can Li, Xiaoyu Zhou, Wei Liu, Jintong Liu, Qiao Lin, Jinglin Huang, Zhihui Hao, Yanyan Jiang, Jiahao Lin","doi":"10.1186/s12951-025-03638-x","DOIUrl":"10.1186/s12951-025-03638-x","url":null,"abstract":"<p><strong>Background: </strong>The limited tumor-specific delivery and insufficient dendritic cell (DC) activation remain critical challenges in cancer immunotherapy. This research aimed to improve antitumor efficacy by developing a novel pH-responsive liposomal nanoplatform that specifically targets DC via Dectin-1 recognition in the tumor microenvironment (TME), thereby enhancing cellular immunity, minimizing off-target toxicity and reprograming the tumor immunosuppressive TME.</p><p><strong>Methods: </strong>The construction, physical stability, biocompatibility and targeting capability of PLP-II/MGlu-Curd-Lips were evaluated using ¹H NMR spectra, FT-IR spectroscopy, TEM, LUMiSizer assay, CCK-8 assay, Flow Cytometry (FC), and IVIS imaging. Therapeutic efficacy was assessed through FC, H&E staining, TUNEL, and immunohistochemical staining. The antitumor mechanism of action of PLP-II/MGlu-Curd-Lips in murine 4T1 breast tumors was investigated using RNA sequencing.</p><p><strong>Results: </strong>A Dectin-1-targeted pH-responsive liposomal nanoplatform (PLP-II/MGlu-Curd-Lips) was developed for spatiotemporally controlled delivery of Plantago asiatica L. acidic polysaccharide (PLP-II). The nanocarrier featured a curdlan-grafted copolymer backbone with pH-cleavable 3-methyl glutarylated moieties and demonstrated an ideal particle size and enhanced stability, enabling tumor acidity-triggered payload release, Dectin-1-mediated DC targeting, and enhanced cytoplasmic delivery via lysosomal escape. The prepared nanocarriers exhibited obvious lysosomal accumulation, and they significantly improved the co-stimulation and migration ability of DCs. In vivo studies indicated that the PLP-II/MGlu-Curd-Lips accumulated at the tumor sites and efficiently promoted DCs activation, tumor-associated macrophages (TAMs) polarization, and cytotoxic T lymphocytes (CTLs) infiltration. Consequently, this remodeling of the tumor microenvironment significantly inhibited the growth of 4T1 breast tumors. Importantly, RNA-Seq confirmed that this therapeutic approach promoted the upregulation of genes related to p53 and NF-κB signaling pathways, thereby enhancing immune activation and tumor-suppression effect.</p><p><strong>Conclusions: </strong>This study establishes curdlan-modified liposomes as the dual-functional nanoplatform that synergistically enhances DC-targeted delivery of PLP-II and systemic immune activation, providing a promising strategy to augment cancer immunotherapy.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"597"},"PeriodicalIF":12.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12400629/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymerase-based DNA reactions for molecularly computing cancerous diagnostic valences of multiple miRNAs.","authors":"Yumin Yan, Hongyang Zhao, Lijie Xing, Ye Ouyang, Linghao Zhang, Jiayu Yang, Jing Qiu, Yongzhong Qian, Liang Ma, Rui Weng, Xin Su","doi":"10.1186/s12951-025-03643-0","DOIUrl":"10.1186/s12951-025-03643-0","url":null,"abstract":"<p><p>Conventional miRNA-based diagnostic methods often treat all biomarkers equally, overlooking the fact that each miRNA contributes differently to disease classification. This differential diagnostic importance is captured by the concept of Cancerous Diagnostic Valence (CDV)-a metric that quantifies both the direction (oncogenic or protective) and magnitude of each miRNA's association with cancer. Here, we introduce a polymerase-based DNA molecular computing system that directly encodes and integrates CDVs to perform weighted molecular classification of non-small cell lung cancer (NSCLC). By coupling DNA polymerase-mediated strand extension and displacement (PB-DSD and cascade PB-DSD), the system translates miRNA inputs into proportional molecular signals spanning a wide CDV range (1-25), with minimal probe complexity. Seven NSCLC-related miRNAs with machine learning-derived CDVs were used to construct a diagnostic classifier, achieving 95% accuracy in tissue and 90% in plasma samples. Compared to conventional toehold strand displacement systems, this approach offers broader scalability, lower background interference, and more accurate diagnostic logic. Furthermore, we demonstrate its utility for therapeutic monitoring by tracking drug-induced shifts in CDV-weighted miRNA profiles in tumor-bearing mice treated with allicin and curcumin. This work establishes a molecularly programmable and biologically informed diagnostic platform that advances the precision and interpretability of miRNA-based cancer diagnostics.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"598"},"PeriodicalIF":12.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12400545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intraoperative application of an antioxidant nanoparticle-hydrogel targeting microglia regulates neuroinflammation in traumatic brain injury.","authors":"Yuhan Han, Jiacheng Gu, Miaomiao Xu, Yufei Ma, Weiji Weng, Qiyuan Feng, Zhenghui He, Wenlan Qi, Qing Mao, Jiyao Jiang, Junfeng Feng","doi":"10.1186/s12951-025-03682-7","DOIUrl":"10.1186/s12951-025-03682-7","url":null,"abstract":"<p><p>Microglia play a critical role in neuroinflammation, a key secondary injury mechanism following traumatic brain injury (TBI). The colony-stimulating factor 1 receptor (CSF-1R) inhibitor PLX5622 has shown promise in suppressing neuroinflammation by depleting microglia, but it lacks specificity in targeting microglia at the injury site. To overcome this limitation, we developed PLX5622 nanoparticles functionalized with the CAQK peptide for lesion-specific targeting and combined them with a hydrogel (GelMA-PPS) that possesses potent reactive oxygen species (ROS) scavenging capabilities. This nanoparticle-hydrogel drug delivery system (GelMA-PPS/P) significantly enhanced the delivery efficiency and therapeutic efficacy of PLX5622 in TBI treatment. Localized administration of this system effectively depleted microglia at the injury site, suppressed neuroinflammation, and reduced the release of inflammatory cytokines. Its ROS scavenging ability was also validated in vitro and in vivo. Together, these effects synergistically improved neurological function recovery in TBI mouse models. This innovative strategy offers a comprehensive and targeted approach to managing neuroinflammation after TBI, providing a promising avenue for advancing TBI therapies.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"599"},"PeriodicalIF":12.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12400644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Defect-engineered amorphous-like nanointerceptors for T₂ MRI-Guided treatment of reperfusion injury.","authors":"Xiaotong Ma, Xin Liang, Yixin Yu, Hao Guan, Xuejiao Gao, Jing Li, Shanyue Guan, Aihua Liu, Li Yao, Kelong Fan","doi":"10.1186/s12951-025-03624-3","DOIUrl":"https://doi.org/10.1186/s12951-025-03624-3","url":null,"abstract":"<p><p>Ischemic reperfusion (I/R) injury is dominated by excessive reactive oxygen species (ROS)-mediated oxidative damage and uncontrolled inflammation, yet effective strategies for simultaneous diagnosis and treatment remain elusive. Herein, we report a defect-engineered amorphous-like MnCeO_x nanointerceptor with dual capabilities of magnetic resonance imaging (MRI) -guided stroke diagnosis and ROS-scavenging therapy. The synergistic effect of the amorphous-like structure and Mn-Ce solid solution induces abundant oxygen vacancies and a disordered surface, significantly boosting ROS catalytic removal. Theoretical calculations confirm that Mn doping and oxygen vacancy formation modulate the electronic structure, reduce the adsorption energy of ROS intermediates, and lower catalytic energy barriers, thereby enhancing enzyme-like activity. As a result, MnCeO_x exhibits an exceptionally high superoxide radical scavenging efficiency (115-fold higher than CeO_x) and superior MRI contrast (r₂ = 139 mM⁻¹) for precise lesion localization. In vivo, MnCeO_x efficiently alleviates ROS-mediated oxidative stress and neuroinflammation, promoting substantial recovery from I/R injury. This work offers a powerful defect-engineering strategy for developing next-generation diagnostic and therapeutic nanozymes.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"596"},"PeriodicalIF":12.6,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12398121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid nanoparticles-mRNA based on the consensus sequences of avian coronavirus S1 and N genes protect animals against multiple viral infections.","authors":"Hongtao Xiao, Wanyan Wu, Siyao Yu, Jiayang Chen, Lijin Lai, Jinlian Ren, Xiaotong Yan, Qiuyan Lin, Libin Chen, Tao Ren","doi":"10.1186/s12951-025-03668-5","DOIUrl":"https://doi.org/10.1186/s12951-025-03668-5","url":null,"abstract":"<p><p>Lipid nanoparticles-mRNA play important roles in SARS-CoV-2 infection control. Avian coronavirus infectious bronchitis virus (IBV) comprises eight genotypes with a lack of cross-protection, causing severe economic losses to the poultry industry. Using immunoinformatics methods, five consensus sequence antigens against prevalent IBV strains were designed. Four monovalent lipid nanoparticles-mRNA (GI-19, GI-13, GI-7, GVI-1) and one quadrivalent lipid nanoparticles-mRNA were constructed to develop a broad-spectrum IBV vaccine. The safety and biodistribution of the lipid nanoparticles-mRNA were evaluated in SPF chickens and confirmed that it induced a strong and durable immune response. The lipid nanoparticles-mRNA efficacy in SPF chickens was verified in infection assays with four genotypes of IBV strains, the results showed that immunization with a 10 µg dose provided complete protection for the chickens, while immunization with a 5 µg dose reduced disease severity, organ damage, and mortality, and inhibited viral replication and shedding. Our results indicate that these Lipid nanoparticles-mRNA are immunogenic and protective in preclinical animal models. These data can provide a basis for IBV prevention and control and the development of mRNA vaccines against other prevalent viruses.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"23 1","pages":"595"},"PeriodicalIF":12.6,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12398015/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}