Journal of Nanobiotechnology最新文献

{"title":"Publisher Correction: Extracellular vesicles inherit lactate from aggregated MSCs to alleviate type 1 diabetes mellitus via H₂S-induced CD8⁺ T cell exhaustion.","authors":"Qianhui Ren, Qianmin Ou, Zhengshi Li, Luhan Niu, Deqian Tang, Xinyu Liu, Xueli Mao, Songtao Shi","doi":"10.1186/s12951-026-04905-1","DOIUrl":"https://doi.org/10.1186/s12951-026-04905-1","url":null,"abstract":"","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"24 1","pages":""},"PeriodicalIF":15.0,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13483483/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148794269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Multifunctional photodynamic/photothermal nano-agents for the treatment of oral leukoplakia.","authors":"Lin Lin, Chuanhui Song, Zheng Wei, Huihui Zou, Shengwei Han, Zichen Cao, Xinyu Zhang, Guorong Zhang, Jianchuan Ran, Yu Cai, Wei Han","doi":"10.1186/s12951-026-04908-y","DOIUrl":"https://doi.org/10.1186/s12951-026-04908-y","url":null,"abstract":"","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"24 1","pages":""},"PeriodicalIF":15.0,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13483666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148794295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor-Targeting monodisperse hydroxyapatite core–shell nanospheres for overcoming multidrug resistance in cancer therapy via tumor-specific synergistic calcium overload","authors":"Shuiquan Zhang, Tao Shen, Wen Zhang, Xue Gong, Yuan Yuan, Changsheng Liu, Jiangchao Qian","doi":"10.1186/s12951-026-04785-5","DOIUrl":"https://doi.org/10.1186/s12951-026-04785-5","url":null,"abstract":"Multidrug resistance (MDR) remains a central obstacle in cancer therapy, accounting for over 90% of cancer-related mortality. Although calcium overload has emerged as a promising anticancer strategy, its clinical translation is largely limited by insufficient tumor selectivity. Hydroxyapatite nanoparticles (HAPNs) possess intrinsic biocompatibility and the ability to induce tumor-specific Ca 2+ overload; however, conventional synthesis methods often yield polydisperse and aggregation-prone particles, resulting in high dosage requirements and inconsistent antitumor efficacy. Herein, we reported a tumor-targeting monodisperse hydroxyapatite core–shell nanospheres (TMHCNs) fabricated via microfluidic coaxial chips (MCC)-assisted soft-templating strategy to achieve precise physicochemical control for tumor-specific synergistic Ca 2+ overload therapy. FDA-approved components, sebacic acid, glycerol, and polyethylene glycol, were used to compose uniform nanomicelles as soft templates for homogeneous mineralization, enabling reproducible generation of uniform core–shell nanospheres. Subsequent surface functionalization with hyaluronic acid (HA) yielded monodisperse tumor-targeting nanospheres, TMHCNs (47.0 ± 5.0 nm) with enhanced CD44-mediated cellular uptake. The antimalarial drug and sarcoplasmic/endoplasmic reticulum Ca 2+ -ATPase (SERCA) inhibitor, artemisinin (Art), was then selected and encapsulated into TMHCNs to construct the synergistic Ca 2+ overload nanosystem Art@TMHCNs. TMHCNs alone induced sustained and tumor-selective intracellular Ca 2+ overload. Loading the SERCA inhibitor Art further amplified tumor-specific Ca 2+ overload, triggered mitochondrial dysfunction, depleted ATP, and activated both intrinsic and extrinsic apoptotic pathways, thereby effectively reversing MDR. In an A549/PTX xenograft model, Art@TMHCNs achieved complete tumor growth suppression and pronounced tumor-specific calcification with minimal off-target toxicity. This work establishes a precisely engineered, monodisperse hydroxyapatite core–shell platform that amplifies tumor-specific calcium dysregulation and provides a clinically translatable strategy for safe and effective MDR reversal in cancer therapy.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148861233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Restoring autophagy-apoptosis balance in diabetic cardiomyopathy via a biomimetic, ROS-responsive nanocarrier associated with Mst1 pathway modulation.","authors":"Xinke Li, Guangwei Li, Nana Meng, Wensi Xu, Runbo Tang, Jianqiang Chen, Lulu Wang, Jun Wang","doi":"10.1186/s12951-026-04903-3","DOIUrl":"10.1186/s12951-026-04903-3","url":null,"abstract":"<p><p>Diabetic cardiomyopathy (DCM) is driven by oxidative stress and an imbalance between autophagy and apoptosis. To improve myocardial delivery of dimethyl fumarate (DMF), a known NRF2-activating redox modulator, and evaluate its association with Mst1 pathway regulation, we developed a cardiac-targeting peptide (APT)-modified biomimetic, reactive oxygen species (ROS)-responsive nanoplatform (NP-APT). NP-APT comprises a ROS-sensitive core and a lipid-cell membrane hybrid coating modified with APT to achieve targeted delivery. The designed nanoplatform demonstrated favorable physicochemical properties, serum/storage stability, macrophage-avoidance capability, and ROS-responsive drug release. In vitro, NP-APT enhanced cardiomyocyte uptake, mitigated high-glucose-induced oxidative stress, restored mitochondrial function and bioenergetic activity, and attenuated apoptosis. Mechanistically, NP-APT protected cardiomyocytes in association with reduced Mst1-related protein abundance, improved autophagy-related signaling, and rebalanced the autophagy-apoptosis equilibrium; Mst1 overexpression weakened these effects, whereas autophagy blockade reduced NP-APT-mediated protection. In a murine DCM model, NP-APT achieved cardiac-specific accumulation, significantly improved cardiac function and fibrosis, and restored cellular homeostasis, with efficacy associated with Mst1 pathway modulation. Hematological, biochemical, behavioral, and histological safety assessments indicated that NP-APT did not produce evident additional systemic toxicity under the tested treatment conditions in DCM mice. Collectively, this study demonstrates that APT-mediated biomimetic nanodelivery provides an effective strategy to enhance DMF myocardial delivery and ameliorate DCM in association with Mst1-autophagy-apoptosis pathway regulation.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"24 1","pages":""},"PeriodicalIF":15.0,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13505037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148818771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced integrated SERS-based strategies for the early diagnosis of upper gastrointestinal cancers.","authors":"Qian Wang, Siyi Jiang, Shuyu Zhai, Wenjia Yin, Yao Sun, Huifang Zhao, Ruiping Zhang","doi":"10.1186/s12951-026-04844-x","DOIUrl":"10.1186/s12951-026-04844-x","url":null,"abstract":"<p><p>Upper gastrointestinal (UGI) cancers are associated with high incidence and poor prognosis due to their insidious onset, posing a serious threat to human health. Traditional diagnostic approaches, including endoscopic biopsy, imaging modalities, and molecular assays, frequently suffer from tedious procedures and insufficient sensitivity. Therefore, it is imperative to develop novel technologies for the early diagnosis of UGI cancers to reduce diagnostic delay, ensure diagnostic accuracy, and ultimately improve patient survival rates. Owing to its exceptional sensitivity and rich molecular fingerprinting information, surface-enhanced Raman spectroscopy (SERS) has emerged as an invaluable analytical tool for uncovering metabolic molecular alterations, thereby facilitating rapid, sensitive, and early-stage diagnosis of UGI cancers. Herein, this review not only outlines the principles and detection strategies of SERS technology, but also highlights its recent advances in the early diagnosis of UGI cancers, particularly in conjunction with other innovative technologies. Ultimately, the current challenges and future development directions are discussed, providing a valuable perspective to guide future efforts toward early UGI cancer screening.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"24 1","pages":""},"PeriodicalIF":15.0,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13508314/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148829348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing chitosan-based biomaterials for advanced cancer immunotherapy: from nanocarriers to tumor microenvironment modulation.","authors":"Yun Wang, Yan Fan, Jing Zhang, Ning Sun, Kang Chen, Qasim Khan, Haishan Zhang","doi":"10.1186/s12951-026-04821-4","DOIUrl":"https://doi.org/10.1186/s12951-026-04821-4","url":null,"abstract":"<p><p>Cancer immunotherapy has transformed oncology, however, its clinical efficacy remains limited by immunosuppressive tumor microenvironments (TMEs), poor therapeutic delivery, systematic toxicity, treatment resistance. Chitosan, a biocompatible and biodegradable polysaccharide, has emerged as a versatile biomaterial capable of addressing these challenges through both intrinsics immunomodulatory activity and advanced drug-delivery functions. This review summarizs recent advances in chitosan-based biomaterials for cancer immunotherapy, highlighting their ability to activate innate and adaptive immune responses through pathways involving patterns recognition receptors, cyclic GMP-AMP synthase (cGAS)=simulator of interferon genes (STING) signaling, dendric cells (DCs)macrophages, natural killer (NK) cells and T lymphocytes. The design and application of chitosan-based nanoparticles and hydrogels as platformsfor delivering cytokines, nucleic acids, immune adjuvant, cancer vaccines and immune checkpoint therapeutics are discussed. Particular attention is given to their roles in TME remodeling, sustained local drug release, postsurgical immunotherapy, and combination approaches that integrate chemotherapy, radiotherapy (RT), phototherapy and immunotherapy. Emerging strategies, including stimuli-responsive systems, biomimetic formulations, and multifunctional nanoplatforms are also discussed. Finally, we discuss the current translational current translational challenges and future perspectives, emphasizing the potential of chitosan-based biomaterials to enhance antitumor immunity and improve clinical outcomes in cancer immunotherapy.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"24 1","pages":""},"PeriodicalIF":15.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148887848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ROS-responsive injectable hydrogel enables controlled release of human adipose tissue-derived extracellular vesicles for multifaceted osteoarthritis therapy.","authors":"Yikai Wang, Le Kang, Kaizhe Chen, Yu Jiang, Yi Zheng, Peng Xu, Chuandong Wang, Wenhui Zhu, Dequn Wu, Kai Liu","doi":"10.1186/s12951-026-04838-9","DOIUrl":"10.1186/s12951-026-04838-9","url":null,"abstract":"<p><p>Chronic inflammation and elevated reactive oxygen species (ROS) are pivotal drivers of osteoarthritis (OA), demanding integrated, pathology-adaptive strategies. Here, we develop an injectable, ROS-responsive hydrogel for intra-articular delivery of human adipose tissue-derived extracellular vesicles (AT-EVs) to enable multifaceted OA treatment. Unlike conventional cell-derived MSC-EVs, AT-EVs are tissue-derived and can be isolated directly from lipoaspirate under aseptic operating-room conditions, providing a clinically practical EV source that bypasses prolonged cell expansion and multi-step culture conditioning. Small RNA-seq established their OA therapeutic potential. To facilitate intra-articular retention, the AT-EVs@BA-CS/EGCG hydrogel is formed via dynamic boronate ester crosslinking between phenylboronic acid-grafted chitosan and epigallocatechin-3-gallate (EGCG), achieving triple functionality: (i) injectable self-healing capacity, (ii) ROS-triggered controlled release, and (iii) synergistic ROS scavenging capacity. In vitro, the hydrogel attenuated oxidative stress, protected chondrocytes, restored matrix homeostasis, and suppressed inflammatory macrophage activation. Integrated small RNA profiling, transcriptomics, and phospho-protein validation consistently implicated PI3K/AKT/mTOR pathway modulation as a key mechanism. In a rat OA model, AT-EVs@BA-CS/EGCG mitigated cartilage degeneration, reduced oxidative damage, and dampened inflammatory macrophage signatures. Collectively, this study provides a clinically practical, ROS-adaptive EV-hydrogel platform with translational potential for OA therapy.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"24 1","pages":""},"PeriodicalIF":15.0,"publicationDate":"2026-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13540952/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mucus and tumor penetrating paclitaxel micelles for potent local therapy of cervical cancer.","authors":"Yijie Chen, Yangla Xie, Jiaping Wu, Xianguo Qu, Youqing Shen, Nasha Qiu, Zhifen Zhang","doi":"10.1186/s12951-026-04528-6","DOIUrl":"10.1186/s12951-026-04528-6","url":null,"abstract":"<p><p>The therapeutic efficacy for cervical cancer treatment is limited by insufficient drug accumulation and penetration due to physiological barriers such as mucin-rich environments after systemic administration. Thus, developing a local drug delivery system is essential to overcome these hindrances. Active transcytosis of cancer nanomedicines holds great promise for enhancing tumor extravasation, infiltration, and antitumor activity. Herein, polyzwitterionic OPDEA-PCL was developed to encapsulate paclitaxel (PTX) into micelles, serving as an intravaginal therapy for orthotopic cervical cancer. The OPDEA-PCL/PTX micelles efficiently penetrated mucus and exhibited strong resistance to mucin fouling, thereby facilitating rapid transcytosis into tumors. Furthermore, OPDEA-PCL/PTX micelles colocalized with the mitochondria of tumor cells, reversing PTX resistance. In the orthotopic cervical tumor model, the inhibition rate of OPDEA-PCL/PTX micelles was 90.0%, more than 2-fold higher than that of free PTX. In the subcutaneous cervical cancer model which is resistant to PTX. Intravenous administration of OPDEA-PCL/PTX micelles significantly overcame PTX resistance, achieving a tumor inhibition rate of 95.2%, and extending the median survival time by more than 2-fold compared to free PTX and PEG-PCL/PTX treated groups. In summary, this approach holds great promise as a potent localized nanomedicine for cervical cancer treatment with minimal side effects.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"24 1","pages":""},"PeriodicalIF":15.0,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13412313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148604272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor microenvironment-responsive nanocarriers for enhanced glioblastoma immunotherapy.","authors":"Cuicui Wang, Zhihong Sun, Jun Sun, Jie Liu, Qi Zhao, Yong Sun, Chengming Sun","doi":"10.1186/s12951-026-04823-2","DOIUrl":"https://doi.org/10.1186/s12951-026-04823-2","url":null,"abstract":"<p><p>The glioblastoma (GBM) microenvironment exhibits a profoundly immunosuppressive state, which constitutes the major barrier limiting the efficacy of immunotherapy. It is intricately intertwined with aberrant physicochemical characteristics including severe hypoxia, acidic pH, and redox imbalance. Although these physicochemical abnormalities further exacerbate immunosuppression within the GBM microenvironment, they also paradoxically serve as precise endogenous triggers for designing smart nanocarriers. By exploiting these pathological features as triggering signals, microenvironment-responsive nanocarriers can overcome the physical barriers imposed by the blood-brain barrier (BBB) and blood-brain tumor barrier (BBTB), enabling precise delivery and on-demand release of immunomodulators at lesion site. Moreover, these nanocarriers can effectively alleviate immune tolerance by reprogramming tumor-associated immune cells or inducing immunogenic cell death, thereby remodeling the immunosuppressive GBM microenvironment. This review elucidates the physicochemical and immunosuppressive features of the GBM microenvironment. Furthermore, it systematically summarizes the design principles, cross-barrier targeting strategies, and immune remodeling mechanisms of responsive nanocarriers engineered upon tumor microenvironment (TME) characteristics. The analysis highlights the synergistic enhancement achieved through this paradigm: responding to TME signals to reverse immunosuppression. Finally, clinical translation challenges and future directions within this field are discussed to provide a comprehensive reference for designing highly efficient, GBM-targeted responsive nanoimmunotherapeutic platforms.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":" ","pages":""},"PeriodicalIF":15.0,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148549583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral delivery of anti-mCD80 nanobody-engineered probiotic bacterial nanovesicles for macrophage-targeted Il17ra silencing in colitis.","authors":"Manli Zhang, Yonggang Zhu, Feng Hu, Min Rao","doi":"10.1186/s12951-026-04811-6","DOIUrl":"https://doi.org/10.1186/s12951-026-04811-6","url":null,"abstract":"<p><p>Inflammatory bowel disease remains challenging to treat because effective intervention requires localized suppression of mucosal inflammation together with restoration of tissue homeostasis. Here, a hierarchically engineered oral nanotherapeutic is developed based on Lactobacillus rhamnosus GG-derived bacterial nanovesicles (BNVs) for inflammatory macrophage-targeted gene silencing in colitis. The vesicles are genetically engineered to display an anti-mCD80 nanobody, loaded with siIl17ra, and further encapsulated within calcium alginate microcapsules to improve gastrointestinal protection and enable gastrointestinal protection and intestinal-fluid-associated release in the lower gut. The resulting system preserves nanoscale vesicular morphology, exhibits favorable cytocompatibility, and shows enhanced uptake by inflammatory macrophages after nanobody decoration. Following internalization, siIl17ra/CD80-BNVs effectively suppress Il17ra expression and reprogram macrophages toward a pro-repair phenotype. Microcapsule incorporation further improves siRNA retention, restrains premature release under acidic conditions, and promotes sustained release under intestinally relevant pH conditions. After oral administration, MC-siIl17ra/CD80-BNVs display enhanced colorectal retention and markedly alleviate dextran sulfate sodium-induced colitis, as evidenced by reduced disease activity, attenuated histopathological injury, enhanced epithelial regeneration, decreased inflammatory mediator expression, and reduced NF-κB/caspase-associated marker changes. This work establishes a microbiota-inspired oral nanomedicine platform for localized immunomodulation and mucosal repair in colitis.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":" ","pages":""},"PeriodicalIF":15.0,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148549556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}