Journal of Nanobiotechnology最新文献

{"title":"Publisher Correction: Extracellular vesicles inherit lactate from aggregated MSCs to alleviate type 1 diabetes mellitus via H₂S-induced CD8⁺ T cell exhaustion.","authors":"Qianhui Ren, Qianmin Ou, Zhengshi Li, Luhan Niu, Deqian Tang, Xinyu Liu, Xueli Mao, Songtao Shi","doi":"10.1186/s12951-026-04905-1","DOIUrl":"https://doi.org/10.1186/s12951-026-04905-1","url":null,"abstract":"","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"24 1","pages":""},"PeriodicalIF":15.0,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13483483/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148794269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Multifunctional photodynamic/photothermal nano-agents for the treatment of oral leukoplakia.","authors":"Lin Lin, Chuanhui Song, Zheng Wei, Huihui Zou, Shengwei Han, Zichen Cao, Xinyu Zhang, Guorong Zhang, Jianchuan Ran, Yu Cai, Wei Han","doi":"10.1186/s12951-026-04908-y","DOIUrl":"10.1186/s12951-026-04908-y","url":null,"abstract":"","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"24 1","pages":""},"PeriodicalIF":15.0,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13483666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148794295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor-Targeting monodisperse hydroxyapatite core–shell nanospheres for overcoming multidrug resistance in cancer therapy via tumor-specific synergistic calcium overload","authors":"Shuiquan Zhang, Tao Shen, Wen Zhang, Xue Gong, Yuan Yuan, Changsheng Liu, Jiangchao Qian","doi":"10.1186/s12951-026-04785-5","DOIUrl":"https://doi.org/10.1186/s12951-026-04785-5","url":null,"abstract":"Multidrug resistance (MDR) remains a central obstacle in cancer therapy, accounting for over 90% of cancer-related mortality. Although calcium overload has emerged as a promising anticancer strategy, its clinical translation is largely limited by insufficient tumor selectivity. Hydroxyapatite nanoparticles (HAPNs) possess intrinsic biocompatibility and the ability to induce tumor-specific Ca 2+ overload; however, conventional synthesis methods often yield polydisperse and aggregation-prone particles, resulting in high dosage requirements and inconsistent antitumor efficacy. Herein, we reported a tumor-targeting monodisperse hydroxyapatite core–shell nanospheres (TMHCNs) fabricated via microfluidic coaxial chips (MCC)-assisted soft-templating strategy to achieve precise physicochemical control for tumor-specific synergistic Ca 2+ overload therapy. FDA-approved components, sebacic acid, glycerol, and polyethylene glycol, were used to compose uniform nanomicelles as soft templates for homogeneous mineralization, enabling reproducible generation of uniform core–shell nanospheres. Subsequent surface functionalization with hyaluronic acid (HA) yielded monodisperse tumor-targeting nanospheres, TMHCNs (47.0 ± 5.0 nm) with enhanced CD44-mediated cellular uptake. The antimalarial drug and sarcoplasmic/endoplasmic reticulum Ca 2+ -ATPase (SERCA) inhibitor, artemisinin (Art), was then selected and encapsulated into TMHCNs to construct the synergistic Ca 2+ overload nanosystem Art@TMHCNs. TMHCNs alone induced sustained and tumor-selective intracellular Ca 2+ overload. Loading the SERCA inhibitor Art further amplified tumor-specific Ca 2+ overload, triggered mitochondrial dysfunction, depleted ATP, and activated both intrinsic and extrinsic apoptotic pathways, thereby effectively reversing MDR. In an A549/PTX xenograft model, Art@TMHCNs achieved complete tumor growth suppression and pronounced tumor-specific calcification with minimal off-target toxicity. This work establishes a precisely engineered, monodisperse hydroxyapatite core–shell platform that amplifies tumor-specific calcium dysregulation and provides a clinically translatable strategy for safe and effective MDR reversal in cancer therapy.","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148861233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A portable nanobiosensor with machine learning: enabling multi-cytokines point-of-care testing and early identification of sepsis immunoparalysis.","authors":"Rongjun Yu, Jiangling Wu, Anyi Chen, Jianjiang Xue, Sihan Guo, Siling Chen, Shanshan Dong, Li Li, Xiao Gui, LiLin Wang, Gang Tian, Gang Liu, Jingfu Qiu, Weixian Chen","doi":"10.1186/s12951-026-04939-5","DOIUrl":"10.1186/s12951-026-04939-5","url":null,"abstract":"<p><p>Sepsis immunoparalysis (SIs) is a major factor contributing to organ dysfunction. However, emergency departments (EDs) and intensive care units (ICUs) currently lack a rapid workflow for early identification of SIs. Herein, an innovative strategy for early SIs identification is proposed. We construct a portable nanobiosensor based on the Nanozyme-Linked Immunosorbent Assay (NLISA) for ultrasensitive and specific Multi-Cytokines quantification, benefiting from its advantages of parallel, independent, rapid, and portable detection. Machine learning is applied to process Multi-Cytokines concentration data measured under varied conditions and build an early identification model for rapid and effective sepsis immunoparalysis diagnosis. This method achieves point-of-care testing (POCT) with small plasma sample volumes (30 µL), with a detection limit as low as 3.8 fg mL^- 1. Notably, machine learning models constructed from different cytokine combinations can achieve high diagnostic performance, accurately distinguishing sepsis immunoparalysis, sepsis non-immunoparalysis, and healthy donors, while addressing diverse clinical needs and cost-effectiveness considerations, enabling personalized diagnostic strategies tailored to distinct patient populations and resource-constrained settings. This opens up the potential application of the nanobiosensor with machine learning for POCT of Multi-Cytokines detection and sepsis immunoparalysis early identification.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"24 1","pages":""},"PeriodicalIF":15.0,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13551774/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148912211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Restoring autophagy-apoptosis balance in diabetic cardiomyopathy via a biomimetic, ROS-responsive nanocarrier associated with Mst1 pathway modulation.","authors":"Xinke Li, Guangwei Li, Nana Meng, Wensi Xu, Runbo Tang, Jianqiang Chen, Lulu Wang, Jun Wang","doi":"10.1186/s12951-026-04903-3","DOIUrl":"10.1186/s12951-026-04903-3","url":null,"abstract":"<p><p>Diabetic cardiomyopathy (DCM) is driven by oxidative stress and an imbalance between autophagy and apoptosis. To improve myocardial delivery of dimethyl fumarate (DMF), a known NRF2-activating redox modulator, and evaluate its association with Mst1 pathway regulation, we developed a cardiac-targeting peptide (APT)-modified biomimetic, reactive oxygen species (ROS)-responsive nanoplatform (NP-APT). NP-APT comprises a ROS-sensitive core and a lipid-cell membrane hybrid coating modified with APT to achieve targeted delivery. The designed nanoplatform demonstrated favorable physicochemical properties, serum/storage stability, macrophage-avoidance capability, and ROS-responsive drug release. In vitro, NP-APT enhanced cardiomyocyte uptake, mitigated high-glucose-induced oxidative stress, restored mitochondrial function and bioenergetic activity, and attenuated apoptosis. Mechanistically, NP-APT protected cardiomyocytes in association with reduced Mst1-related protein abundance, improved autophagy-related signaling, and rebalanced the autophagy-apoptosis equilibrium; Mst1 overexpression weakened these effects, whereas autophagy blockade reduced NP-APT-mediated protection. In a murine DCM model, NP-APT achieved cardiac-specific accumulation, significantly improved cardiac function and fibrosis, and restored cellular homeostasis, with efficacy associated with Mst1 pathway modulation. Hematological, biochemical, behavioral, and histological safety assessments indicated that NP-APT did not produce evident additional systemic toxicity under the tested treatment conditions in DCM mice. Collectively, this study demonstrates that APT-mediated biomimetic nanodelivery provides an effective strategy to enhance DMF myocardial delivery and ameliorate DCM in association with Mst1-autophagy-apoptosis pathway regulation.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"24 1","pages":""},"PeriodicalIF":15.0,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13505037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148818771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"cRGD-functionalized exosome-Mn₃O₄ nanoplatform enhanced ischemic lesion accumulation and ros scavenging-mediated neuroprotection in ischemic stroke.","authors":"Xu Deng, Xixiang Xie, Xing Zhou, Lanfang Jiang, Tao Zhu, Rixiang Su, Chunmei Chen, Wan Chen, Chunxia Chen","doi":"10.1186/s12951-026-04922-0","DOIUrl":"10.1186/s12951-026-04922-0","url":null,"abstract":"<p><p>The therapeutic efficacy of ischemic stroke (IS) treatment is severely limited by insufficient accumulation of therapeutic agents within ischemic lesions and persistent secondary injury after ischemia-reperfusion. Herein, we report a cRGD-functionalized exosome-based nanoplatform that enhances ischemic lesion-associated accumulation and antioxidative neuroprotection for the treatment of IS. Neural stem cell-derived exosomes were functionalized with cyclic RGD peptides (cRGD) and subsequently loaded with Mn₃O₄ nanoparticles to construct a hybrid nanosystem (cRGD-Exo@Mn₃O₄). The engineered exosomes preserve intrinsic brain tropism, while cRGD modification promotes preferential accumulation in ischemic regions, potentially through interaction with α_vβ₃ integrin that is upregulated in ischemic lesions. The incorporated Mn₃O₄ nanoparticles confer robust reactive oxygen species (ROS) scavenging capability, thereby mitigating oxidative stress in ischemic microenvironments. In vitro and in vivo studies demonstrate that cRGD-Exo@Mn₃O₄ exhibits enhanced accumulation in ischemic regions compared with non-modified counterparts. The nanosystem effectively attenuates oxidative stress and neuroinflammation, leading to reduced infarct volume, alleviation of cerebral edema, and improved neurological function in MCAO/R mice. Mechanistically, transcriptomic analysis suggests that the therapeutic effects are associated with modulation of inflammation-and cell death-related pathways, including suppression of the RIPK1/RIPK3/MLKL signaling cascade. Collectively, this study presents a rationally designed exosome-based nanoplatform integrating ischemic lesion-associated accumulation with ROS-scavenging capability.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"24 1","pages":""},"PeriodicalIF":15.0,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13595739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A tumor microenvironment acid-responsive therapy combining fenton chemistry and chemotherapy via TNF pathway for synergistic and safe lung cancer treatment.","authors":"Cai Zhang, Long Zhang, Jing Tian, Jie Shi, Xiao Li, Tong Jiao, Xin Zhao, Bo Yuan, Lizhong Zeng, Yuanyuan Cheng, Umid Sunnatovich Mamedov, Laziz Nurkhonovich Niyazov, Shuanying Yang","doi":"10.1186/s12951-026-04932-y","DOIUrl":"10.1186/s12951-026-04932-y","url":null,"abstract":"<p><p>Lung cancer is one of the most common malignant tumors, but traditional treatments, particularly chemotherapy, often face significant challenges, including drug resistance and high systemic toxicity. In this study, we developed a novel acid responsive nanoparticle, La₂O₃@PDA/PTX, by combining lanthanum oxide (La₂O₃), polydopamine (PDA), and paclitaxel (PTX). This nanoparticle demonstrated excellent aqueous stability, high biocompatibility, and low toxicity. Benefiting from its structural design, the nanoparticle enables targeted delivery of PTX while simultaneously elevating intracellular reactive oxygen species (ROS) levels, which synergistically exacerbates oxidative stress injury within tumor cells. Mechanistically, La₂O₃@PDA/PTX inhibited tumor growth and induced apoptosis by increasing intracellular ROS, inducing G2/M cell-cycle arrest, and regulating proliferation and apoptosis related proteins, including Ki67, Bcl-2, and Bax. At the signaling level, La₂O₃@PDA/PTX increased NF-κB p65 phosphorylation and modulated TNF/NF-κB associated signaling, accompanied by changes in tumor associated cytokines and T-cell associated immunofluorescence signals. Furthermore, it effectively inhibited the progression of both subcutaneous and metastatic tumors with no significant adverse effects on major organs or peripheral blood parameters, showing great promise for future translational research. Collectively, this work presents an acid responsive therapeutic strategy combining ROS enhancement, chemotherapy, and TNF/NF-κB associated signaling modulation for lung cancer treatment.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"24 1","pages":""},"PeriodicalIF":15.0,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13595560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phototherapy-responsive nanoplatform with hydrogel delivery potentiates immunotherapy and inhibits lung metastasis in osteosarcoma via synergistic glycolysis inhibition and vascular normalization.","authors":"Mengchao Dong, Tingting Zhang, Yichun Huang, Meng Liu, Yuhui Yuan, Bowen Liu, Qiang Ma, Haotian Zhang, Canhua Huang, Chengcheng Shi, Yi Zhang","doi":"10.1186/s12951-026-04906-0","DOIUrl":"10.1186/s12951-026-04906-0","url":null,"abstract":"<p><p>The immunosuppressive tumor microenvironment (ITME)-marked by acidosis and hypoxia-persists as a critical barrier to cancer immunotherapy, especially for osteosarcoma. Herein, an injectable pH-responsive nanocomposite hydrogel (OP@NPs) is presented to overcome this challenge via coordinated metabolic modulation, vascular normalization, and immune reprogramming. This platform co-delivers a novel lactate metabolism inhibitor (D780, a derivative conjugated by IR780 and diclofenac), Zn²⁺, and the anti-angiogenic agent nintedanib (NIB). D780 inhibits key glycolytic enzymes to reduce lactate production, while exerting photothermal/photodynamic effects that directly ablate tumors and mitigate hypoxia. Through metal coordination, Zn²⁺ assembles NIB and D780 into NPs. It also blocks GLUT1, boosting D780's inhibition of glycolysis. NIB can restore abnormal vascular structure, thereby alleviating oxidative hypoxia and blocking epithelial-mesenchymal transition. In murine osteosarcoma models, the combined therapy centered on OP@NPs remodelled the tumor immune microenvironment (TME). This transformation led to an accumulation of cytotoxic T cells, dendritic cells and M1 macrophages. The treatment achieved favorable outcomes in inhibiting primary tumor growth and pulmonary metastases. Overall, this work presents a localized targeting strategy to regulate key signaling pathways in osteosarcoma.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"24 1","pages":""},"PeriodicalIF":15.0,"publicationDate":"2026-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13551941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148912255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cationic nanoemulsions enable follicle-associated delivery with reduced systemic exposure for combined minoxidil/finasteride therapy against androgenetic alopecia","authors":"Ming-Wei Huo, Jing-Yi Liu, Ye-Chao Zhang, Xin Feng, Le-Yan Gu, Cai-Yi Ping, Yan-Kai Qu, Honghao Pan, Beizhen Guo, Bihe Jin, Yongan Tang, Qing-Ri Cao","doi":"10.1186/s12951-026-04902-4","DOIUrl":"https://doi.org/10.1186/s12951-026-04902-4","url":null,"abstract":"","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":" ","pages":""},"PeriodicalIF":15.0,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148909380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breaking the ROS inflammation loop: a hierarchical nanozyme natural enzyme cascade for acetaminophen induced acute liver injury.","authors":"Mengmeng Dong, Xiaomin Huang, Kaiqiang Li, Xiaorong Zhu, Tingting Hu, Xiaohuan Guo, Ke Hao, Fei Kong, Mei Yang, Ning Hu, Yingwei Wang, Lisen Lu, Zhen Wang","doi":"10.1186/s12951-026-04912-2","DOIUrl":"10.1186/s12951-026-04912-2","url":null,"abstract":"<p><p>Acetaminophen (APAP) induced acute liver injury (ALI) is driven by a self amplifying cascade of reactive oxygen species (ROS) and lipid peroxidation, yet current therapies fail to interrupt this pathological loop. Here we engineer a liver targeted hierarchical nanozyme, SOD@ZIF-8/Mn₃O₄, which integrates natural superoxide dismutase (SOD) and Mn₃O₄ nanozymes within a ZIF‑8 nanoplatform. Unlike conventional single step scavengers, this system implements a catalytic hierarchy matching strategy. SOD rapidly converts O₂·^- to H₂O₂, while Mn₃O₄ simultaneously decomposes H₂O₂ and intercepts ·OH, thereby converting the entire ROS cascade into benign water. Density functional theory calculations reveal that the SOD-Mn₃O₄ interface lowers the energy barriers for catalase like and glutathione peroxidase like steps, establishing a synergistic channeling effect. In APAP injured AML-12 cells and a murine ALI model, the nanozyme not only alleviates oxidative stress and lipid peroxidation but also breaks the ROS inflammation feed forward loop by restoring the Keap1/Nrf2/HO‑1 redox switch and suppressing NF‑κB activation. Transcriptomic and functional analyses confirm that treatment rewires hepatic transcriptional programs associated with lipid metabolism and inflammatory signaling. This work establishes a modular nanozyme natural enzyme hybrid platform where the MOF acts as a spatiotemporal organizer, transforming the pathological ROS chain into a controlled detoxification pathway. The concept is readily extensible to other diseases driven by sequential ROS cascades, offering a paradigm shift from single enzyme mimicry to pathway reprogramming nanobiocatalysis.</p>","PeriodicalId":16383,"journal":{"name":"Journal of Nanobiotechnology","volume":"24 1","pages":""},"PeriodicalIF":15.0,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13595542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}