Journal of Medical Virology最新文献_第6页

Highly Sensitive and Specific Diagnosis of Enterovirus A71 by Reverse Transcription Multiple Cross-Displacement Amplification-Labeled Nanoparticles Biosensor 利用反转录多重交叉置换扩增标记纳米粒子生物传感器对肠病毒 A71 进行高灵敏度和特异性诊断

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2024-11-12 DOI: 10.1002/jmv.70059

Jinzhi Cheng, Yuhong Zhou, Xiaomin Tang, Jingrun Lu, Yu Wang

{"title":"Highly Sensitive and Specific Diagnosis of Enterovirus A71 by Reverse Transcription Multiple Cross-Displacement Amplification-Labeled Nanoparticles Biosensor","authors":"Jinzhi Cheng, Yuhong Zhou, Xiaomin Tang, Jingrun Lu, Yu Wang","doi":"10.1002/jmv.70059","DOIUrl":"10.1002/jmv.70059","url":null,"abstract":"<div>\u0000 \u0000 Enterovirus A71 (EVA71) is a leading causative agent of hand, foot, and mouth disease, posing a significant threat to the health of young children, particularly in the Asia-Pacific region. Currently, there is no specific antiviral drug for EVA71 infection; therefore, early and rapid diagnosis is critical for disease prevention and control. Here, we report the development of a simple, rapid, and sensitive detection method for EVA71 infection using reverse transcription-multiple cross displacement amplification (RT-MCDA) combined with nanoparticle-based lateral flow biosensors (LFB). In the RT-MCDA system, a set of 10 primers was designed to target the highly conserved region of the VP1 gene of EVA71 and amplify the genes in an isothermal amplification device. The RT-MCDA amplification reaction products could then be identified by visual detection reagent (VDR) and LFB without the need for specialized equipment. The results demonstrated that the optimal reaction condition for the EVA71-RT-MCDA assay was 65℃ for 40 min. The EVA71-RT-MCDA assay could detect as low as 40 copies of plasmid and 50 copies of pseudotyped virus in a reaction. No cross-reaction was found between EVA71 strains and non-EVA71 strains. For 125 clinical anal swab samples, with EVA71-RT-MCDA assay, 30 samples were positive, which was in consistent with the the conventional real-time quantitative reverse transcription polymerase chain reaction assays. The entire procedure, including a 15-min specimen processing step, a 40-min MCDA reaction, and result reporting within 2 min, was completed in less than 60 min. In conclusion, the EVA71-RT-MCDA-LFB assay targeting the VP1 gene is a rapid, highly sensitive, simple, and specific test that could be widely applied in point-of-care settings and basic medical facilities in rural areas.</div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"96 11","pages":""},"PeriodicalIF":6.8,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Measles Surveillance and Vaccination in 2005–2021 in Guangdong, China 中国广东省 2005-2021 年麻疹监测和疫苗接种情况。

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2024-11-12 DOI: 10.1002/jmv.70045

Jiufeng Sun, Zhaowan Li, Jian Liang, Xin Xie, Guanghu Zhu, Limei Sun

引用次数: 0

HBV Antigen-Guided Switching Strategy From Nucleos(t)ide Analogue to Interferon: Avoid Virologic Breakthrough and Improve Functional Cure 从核苷酸类似物到干扰素的 HBV 抗原指导转换策略：避免病毒学突破并改善功能性治愈。

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2024-11-12 DOI: 10.1002/jmv.70021

Da Huang, Zhize Yuan, Di Wu, Wei Yuan, Jiang Chang, Yuying Chen, Qin Ning, Weiming Yan

{"title":"HBV Antigen-Guided Switching Strategy From Nucleos(t)ide Analogue to Interferon: Avoid Virologic Breakthrough and Improve Functional Cure","authors":"Da Huang, Zhize Yuan, Di Wu, Wei Yuan, Jiang Chang, Yuying Chen, Qin Ning, Weiming Yan","doi":"10.1002/jmv.70021","DOIUrl":"10.1002/jmv.70021","url":null,"abstract":"Little is known for factors associated with virologic breakthrough (VBT) after switching from nucleos(t)ide analogue (NA) to pegylated interferon alpha (Peg-IFN-α) for patients with chronic hepatitis B (CHB). Eighty patients who received 48-week Peg-IFN-ɑ and NA combination therapy followed by Peg-IFN-ɑ monotherapy for additional 48 weeks were included in this study. HBV-related markers including HBV DNA, HBsAg, HBcrAg, HBeAg, cccDNA, and immunological biomarkers were dynamically evaluated. Twelve (15.0%) patients experienced VBT after switching to Peg-IFN-ɑ and exhibited significantly lower rates of HBsAg loss after therapy completion (0% vs. 35.3%, p = 0.014). The patients with HBcrAg≥ 5 log10U/mL and HBsAg≥ 100 IU/mL had the highest risk of VBT and failed to achieve subsequent HBsAg clearance. Intrahepatic cccDNA level was significantly higher in patients with HBcrAg≥ 5 log10U/mL than those with HBcrAg< 5 log10U/mL. Notably, in contrast to patients with HBcrAg< 5 log10U/mL or with HBsAg< 100 IU/mL who had obviously restored HBV-specific CD8+T cell, Tfh or B cell responses before NA cessation, those with HBcrAg≥ 5 log10U/mL or with HBsAg≥ 100 IU/mL exhibited lackluster immunities before NA cessation and notable diminished immune responses thereafter. Monitoring HBcrAg and HBsAg levels, which correlated with poor immune responses during sequential Peg-IFN-ɑ strategy, may help to avoid VBT and improve functional cure of CHB.","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"96 11","pages":""},"PeriodicalIF":6.8,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep sequencing of dengue virus genome reveals simultaneous circulation and coinfection of dengue virus in Southern Brazil 2022–2023 登革热病毒基因组深度测序显示，2022-2023 年登革热病毒在巴西南部同时流行并合并感染。

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2024-11-12 DOI: 10.1002/jmv.70013

Thales Bermann, Ludmila F. Baethgen, Tatiana S. Gregianini, Fernanda Godinho, Regina B. Barcellos, Amanda P. Ruivo, Milena Bauerman, Taina M. Selayaran, Franciellen M. dos Santos, Julio A. Schoerer, Érica B. Möllmann, Fernanda L. Martiny, Valeska L. Lagranha, Luiz F. V. de Oliveira, Ana B. G. da Veiga, Andreia C. Turchetto-Zolet, Gabriel L. Wallau, Richard S. Salvato

引用次数: 0

Impact of Tobacco Use on Herpes Simplex Virus Infections: Findings From a National Survey 烟草使用对单纯疱疹病毒感染的影响：一项全国调查的结果。

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2024-11-12 DOI: 10.1002/jmv.70042

Jiafeng Zhang, Shuangxia Zhang, Huiquan Wang, Meng Sun, Yunxia Zhu, Lin Zhou

{"title":"Impact of Tobacco Use on Herpes Simplex Virus Infections: Findings From a National Survey","authors":"Jiafeng Zhang, Shuangxia Zhang, Huiquan Wang, Meng Sun, Yunxia Zhu, Lin Zhou","doi":"10.1002/jmv.70042","DOIUrl":"10.1002/jmv.70042","url":null,"abstract":"<div>\u0000 \u0000 Herpes simplex virus (HSV) infections, primarily caused by HSV-1 and HSV-2, are prevalent worldwide and carry significant health implications. The impact of tobacco use on HSV infections, however, remains underexplored. This cross-sectional study utilized the National Health and Nutrition Examination Survey (NHANES) database (2009–2016) to investigate the link between Tobacco use and HSV infections among U.S. adults. Smoking status, volume, and serum cotinine levels were analyzed, alongside demographic and behavioral factors. Propensity score matching (PSM) was employed to adjust for confounders such as sexual behavior. Our study involved 5693 participants to explore the relationship between tobacco use and HSV infection. We found that smokers, particularly current smokers, have a significantly increased risk of both HSV-1 and HSV-2 infections compared to non-smokers. Specifically, the adjusted odds ratio (OR) for HSV-1 in current smokers was 1.36 (95% CI: 1.16–1.59, p < 0.001), and for HSV-2, it was 2.37 (95% CI: 1.88–3, p < 0.001). The risk escalates with the intensity of smoking. Elevated serum cotinine levels correlated with an increased risk of HSV infection (HSV-1:1.13 [95% CI:1.09–1.18, p < 0.001]; HSV-2:1.33 [95% CI:1.25–1.41, p < 0.001]). After PSM for factors such as age, gender, sexual behavior, and condom use, these associations remained significant. Tobacco use is significantly associated with an increased risk of HSV infections, highlighting the importance of reducing tobacco exposure in public health strategies against HSV. Further, longitudinal studies are warranted to establish causality and explore underlying mechanisms.</div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"96 11","pages":""},"PeriodicalIF":6.8,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transmission Network and Phylogenetic Analysis Highlight the Role of Suburban Population in HIV-1 Transmission Among Older Adults in Nanjing, Jiangsu Province, China 传播网络和系统发育分析凸显郊区人口在中国江苏省南京市老年人HIV-1传播中的作用。

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2024-11-12 DOI: 10.1002/jmv.70035

Xin Li, Hui Shi, Hongjie Shi, Yuanyuan Xu, Sushu Wu, Rong Wu, Xin Yuan, Jingwen Wang, Zhengping Zhu

{"title":"Transmission Network and Phylogenetic Analysis Highlight the Role of Suburban Population in HIV-1 Transmission Among Older Adults in Nanjing, Jiangsu Province, China","authors":"Xin Li, Hui Shi, Hongjie Shi, Yuanyuan Xu, Sushu Wu, Rong Wu, Xin Yuan, Jingwen Wang, Zhengping Zhu","doi":"10.1002/jmv.70035","DOIUrl":"10.1002/jmv.70035","url":null,"abstract":"<div>\u0000 \u0000 Describing the transmission characteristics among older adults is essential for designing tailored interventions. An epidemiological investigation combined with phylogenetic analysis was conducted to reveal potential transmission linkages among older adults in Nanjing. Between 2018 and 2022, 188 pol sequences were successfully amplified. Multiple genotypes were identified, including CRF07_BC (55.3%), CRF01_AE (30.3%), CRF08_BC (8.0%), B (3.2%), CRF55_01B (1.1%), CRF67_01B (0.5%), CRF68_01B (0.5%), and unique recombinant forms (URF) (1.1%). Transmission network analysis identified 120 genetically linked patients forming 23 clusters, ranging from 2 to 26 individuals. Multivariable logistic regression analysis showed that compared with farmers and heterosexuals, patients with other occupations (OR = 0.404, 95% CI: 0.173−0.945) and MSM (OR = 0.193, 95% CI: 0.050−0.738) were less likely to have high linkage. Subjects who lived in suburban areas were more likely to have high linkage (OR = 10.932, 95% CI: 3.335−35.830). The Sankey diagram suggested that patients living in suburban areas primarily transmitted the disease within the local district (χ2 = 24.192, p < 0.001). Among the 188 pol sequences, the prevalence of pretreatment drug resistance was 8%. In suburban areas with a rising HIV-1 epidemic, improving early detection and timely treatment is critical. More tailored interventions for this subgroup are urgently needed.</div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"96 11","pages":""},"PeriodicalIF":6.8,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Associations of Lipid Profiles With the Onset of HEV-Related Acute Liver Failure: A Multicenter Cohort Study 血脂谱与 HEV 引起的急性肝衰竭发病的关系：一项多中心队列研究

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2024-11-11 DOI: 10.1002/jmv.70033

Rui Dong, Hong Xue, Lin Chen, Wenjuan Jin, Zhenghan Luo, Chao Shen, Lili Huang, Jianguo Shao, Jie Wang

{"title":"Associations of Lipid Profiles With the Onset of HEV-Related Acute Liver Failure: A Multicenter Cohort Study","authors":"Rui Dong, Hong Xue, Lin Chen, Wenjuan Jin, Zhenghan Luo, Chao Shen, Lili Huang, Jianguo Shao, Jie Wang","doi":"10.1002/jmv.70033","DOIUrl":"10.1002/jmv.70033","url":null,"abstract":"<div>\u0000 \u0000 Hepatitis E virus (HEV) is one of the major etiologies for acute liver failure. This multicenter retrospective cohort study aimed to investigate the associations of lipid profiles with the risk of HEV-related acute liver failure (HEV-ALF) among hospitalized patients with acute hepatitis E. A total of 1061 participants were obtained from three tertiary medical centers in Jiangsu, China, between February 2018 and May 2024. Univariate and multivariate Cox regression models were constructed to assess the associations between lipid profiles and the risk of HEV-ALF onset. The time-dependent area under the receiver-operating-characteristic curve (AUROC) and decision curve analysis were used to further evaluate the predictive value of blood lipids. After adjusting for potential confounders, total cholesterol (HR = 0.535, 95% CI: 0.437–0.656, p < 0.001), high-density lipoprotein-cholesterol (HR = 0.065, 95% CI: 0.027–0.154, p < 0.001), low-density lipoprotein-cholesterol (HR = 0.653, 95% CI: 0.512–0.833, p = 0.001), and apolipoprotein A (ApoA) (HR = 0.006, 95% CI: 0.002–0.020, p < 0.001) were significantly associated with a reduced risk of HEV-ALF. Moreover, blood ApoA exhibited excellent discrimination ability and net benefit for predicting 7-day (AUROC = 82.47%, 95% CI: 77.92–87.02) and 14-day (AUROC = 78.81%, 95% CI: 74.13–83.49) HEV-ALF onset. The findings may provide further evidence on the progression of HEV infection and future risk prediction.</div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"96 11","pages":""},"PeriodicalIF":6.8,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Burden and seasonality of RSV bronchiolitis in hospitalized children on a French Caribbean island: Practical lessons from a 13-year study 法属加勒比海岛住院儿童 RSV 支气管炎的负担和季节性：13 年研究的实用经验。

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2024-11-11 DOI: 10.1002/jmv.70006

Olivier Fléchelles, Camille Oger, Aude Charollais, Moustapha Drame, Rishika Banydeen, Fatiha Najioullah

{"title":"Burden and seasonality of RSV bronchiolitis in hospitalized children on a French Caribbean island: Practical lessons from a 13-year study","authors":"Olivier Fléchelles, Camille Oger, Aude Charollais, Moustapha Drame, Rishika Banydeen, Fatiha Najioullah","doi":"10.1002/jmv.70006","DOIUrl":"10.1002/jmv.70006","url":null,"abstract":"Epidemiology of respiratory syncytial virus (RSV) bronchiolitis in the tropics is poorly understood, complicating the effective management of RSV epidemics. The main objective was to describe the seasonality of RSV bronchiolitis epidemics and the clinical characteristics of hospitalized infants over a 13-year period on the French Caribbean island of Martinique. Single-center retrospective observational study including infants under 2 years of age hospitalized at the Martinique University Hospital for RSV-positive bronchiolitis from January 2007 to December 2019. One thousand two hundred thirty-tree cases were included. Epidemics occurred during the rainy season, beginning in September, peaking in October/November and ending in December. A distinct biennial seasonality pattern was observed, with alternating years of high and low incidence. Mean duration of epidemics was of 11 weeks. Clinical characteristics of patients were similar to those hospitalized in temperate areas. Median hospital stay was 4 days. Median age was 3 months, 14.1% of patients were born prematurely, 2.5% had congenital heart disease and 41.1% required oxygen therapy. In Martinique, RSV bronchiolitis epidemics in infants occur in a regular biennial pattern during the rainy season. An accurate knowledge of the local seasonality will allow us to better anticipate hospital organization before epidemics.","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"96 11","pages":""},"PeriodicalIF":6.8,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BIC/FTC/TAF Benefits People Living With HIV After Omicron Breakthrough Infection in Shortening Duration of Symptoms, Enhancing Specific Immune Response and Increasing Total CD4 Cells BIC/FTC/TAF 在缩短症状持续时间、增强特异性免疫反应和增加 CD4 细胞总数方面，有益于 Omicron 突破性感染后的 HIV 感染者。

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2024-11-11 DOI: 10.1002/jmv.70036

Ni Wang, Qing Lin, Huimin Fan, Yixuan Wang, Chang Shu, Na Wang, Dazhi Zhang

{"title":"BIC/FTC/TAF Benefits People Living With HIV After Omicron Breakthrough Infection in Shortening Duration of Symptoms, Enhancing Specific Immune Response and Increasing Total CD4 Cells","authors":"Ni Wang, Qing Lin, Huimin Fan, Yixuan Wang, Chang Shu, Na Wang, Dazhi Zhang","doi":"10.1002/jmv.70036","DOIUrl":"10.1002/jmv.70036","url":null,"abstract":"The clinical manifestations of variability in PLWH with SARS-CoV-2 acquired may be attributed to the use of ART drugs. Omicron breakthrough infection and reinfection in 27 PLWH treated with BIC/FTC/TAF and 51 PLWH treated with TDF/3TC/EFV were investigated, and humoral and cellular immunity after Omicron breakthrough infection were also compared. The results showed that Omicron breakthrough infection symptoms in BIC/FTC/TAF-treated had a shorter duration, and BIC/FTC/TAF could further reduce the duration of symptoms when re-infected. It was also found that the BA.5 specific IgG titers of BIC/FTC/TAF-treated were significantly increased and increased with the extension of the duration of BIC/FTC/TAF. In cellular immunity, the use of BIC/FTC/TAF was also found to increase not only BA.5-specific B lymphocytes and BA.5-specific CD4+T lymphocytes, but also total CD4+T cells. In conclusion, compared to TDF/3TC/EFV, BIC/FTC/TAF can help PLWH shorten the duration of COVID−19 symptoms, enhance SARS-CoV-2 specific humoral and cellular immune responses, help to expand the total CD4+T cells increase in PLWH after Omicron infection and may be more beneficial for PLWH with high risk of infection in the context of COVID-19 normalization.","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"96 11","pages":""},"PeriodicalIF":6.8,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70036","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Rotavirus Genotypes in Children under Five Years in the United Arab Emirates Using Nanopore Sequencing Technology 利用纳米孔测序技术识别阿拉伯联合酋长国五岁以下儿童的轮状病毒基因型。

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2024-11-08 DOI: 10.1002/jmv.70056

Junu A. George, Farah Al-Marzooq, Hassib Narchi, Ahmed R. Alsuwaidi

{"title":"Identification of Rotavirus Genotypes in Children under Five Years in the United Arab Emirates Using Nanopore Sequencing Technology","authors":"Junu A. George, Farah Al-Marzooq, Hassib Narchi, Ahmed R. Alsuwaidi","doi":"10.1002/jmv.70056","DOIUrl":"10.1002/jmv.70056","url":null,"abstract":"Group A rotaviruses (RVA) remain a principal cause of childhood diarrhea in the UAE, despite universal vaccine use. Monitoring genetic diversity is important for identifying prevalent genotypes and escape mutants. Although real-time polymerase chain reaction (RT-PCR) is widely used for RVA genotyping, it may not detect some new strains. This study evaluates nanopore sequencing and RT-PCR for RVA genotyping. Thirty-three RVA strains from children under 5 years presenting with diarrhea were genotyped using both methods. Thirteen strains were genotyped by RT-PCR and confirmed by nanopore sequencing. Fifteen strains were genotyped by nanopore method alone. Most PCR-genotyped strains (56%) had the VP7 G9 genotype, with G3 in five strains and G12 in two. For VP4, P8 (n = 8) and P4 (n = 7) were dominant. The most frequent combinations were G9P[8] (31%) and G9P[4] (25%). Nanopore sequencing of 28 strains revealed G3P[8] (29%) as the most prevalent, followed by G8P[8] (18%). G9P[8] and G2P[4] were present in 14% of samples with G12P[6] being the rarest (7%). Other combinations were detected in 4% the specimens with one nontypeable. Nanopore sequencing was superior to PCR in identifying diverse and emerging genotypes like G8P[8]. This method may enhance surveillance studies and guide preventive measures for RVA gastroenteritis.","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"96 11","pages":""},"PeriodicalIF":6.8,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70056","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0