Electroanalysis最新文献

{"title":"Electrochemical Monitoring of the Impact of Antibiotic Agents on Pseudomonas aeruginosa Phenazine Metabolite Production Rates","authors":"Olivia M. Clay, Courtney J. Weber, Richard S. Anum, Olja Simoska","doi":"10.1002/elan.12021","DOIUrl":"https://doi.org/10.1002/elan.12021","url":null,"abstract":"<p><i>Pseudomonas aeruginosa</i> is an opportunistic pathogen responsible for a high percentage of nosocomial infections, particularly posing a threat to immunocompromised patients. The increased use of antibacterial agents has led to the emergence of antibiotic resistance mechanisms in this pathogen, exacerbating challenges in treating <i>P. aeruginosa</i> infections. Previous studies have correlated the endogenous production of phenazines in <i>P. aeruginosa</i> to the development of antibiotic resistance in the bacterium. Phenazines are nitrogen-containing heterocyclic compounds endogenously produced by <i>P. aeruginosa</i> as redox-active biogenic signaling molecules during early infection stages. While several studies have provided qualitative insights into the effects of different antibiotics on <i>P. aeruginosa</i> growth, quantitative reports on the changes in <i>P. aeruginosa</i> cellular communication and metabolite production dynamics following antibiotic exposure are limited. Addressing this knowledge gap, this study reports real-time electrochemical quantification of phenazine virulence metabolites in both wild-type PA14 and clinical PA2114 strains of <i>P. aeruginosa</i> with varying levels of antibiotic resistance. Specifically, the impact of antibacterial agent ciprofloxacin (CIP) at different concentrations (0, 0.05, and 1 mg L<sup>−1</sup> CIP) on phenazine production in these <i>P. aeruginosa</i> strains was electrochemically monitored directly from bacterial cultures over a 48-h period. Square-wave voltammetry was utilized for quantitative monitoring of two phenazines, namely pyocyanin (PYO) and 5-methylphenazine-1-carboxylic acid (5-MCA), correlating electrical current signal at a unique redox potential to the phenazine concentration. The results indicate distinct phenazine redox responses of the clinical isolate PA2114 strain and wild-type PA14 strain due to antibiotic stress. Under optimal cell growth conditions without CIP exposure, the PA2114 strain with higher antibiotic resistance produced increasing PYO concentrations over 48 h, peaking at 84 ± 9 μM PYO. In contrast, the PYO levels produced by the wild-type strain plateaued and decreased after 24 h of growth, reaching a maximum PYO concentration of 65 ± 3 μM. Exposure to 0.05 mg L<sup>−1</sup> CIP led to marginally higher PYO concentrations in the clinical strain (88 ± 9 μM) while the wild-type strain displayed a notable decrease in PYO concentration (54 ± 4 μM). Additionally, cellular growth with 1 mg L<sup>−1</sup> CIP resulted in a decreased maximum PYO concentration for the clinical strain (70 ± 3 μM) and a substantially decreased PYO concentration for the wild-type strain (11 ± 5 μM). These differences in PYO production are attributed to distinctive changes in phenazine biosynthesis dynamics in different <i>P. aeruginosa</i> strains and the addition of CIP. Furthermore, this study shows the monitoring of phenazine biosynthesis dynamics through the detection of the","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Electrochemical Platform Based on Cadmium (II) (fluorophenyl)Porphyrin and Gold Nanoparticles Modified Screen-Printed Electrode Electrode for the Sensitive Detection of Bisphenol A","authors":"Fatma Rejab,&nbsp;Nour Elhouda Dardouri,&nbsp;Ahlem Rouis,&nbsp;Mosaab Echabaane,&nbsp;Habib Nasri,&nbsp;Hamdi Ben Halima,&nbsp;Nicole Jaffrezic-Renault","doi":"10.1002/elan.12017","DOIUrl":"https://doi.org/10.1002/elan.12017","url":null,"abstract":"<p>Bisphenol A (BPA) is categorized as an endocrine-disrupting substance; it is a risky substance to both environmental and human health, necessitating monitoring. In this present study, a novel electrochemical sensing platform based on Cd metalloporphyrin ([(5,10,15,20- tetrakis(p-flurophenyl) porphyrinato] cadmium (II) [CdTFPP]) and gold nanoparticles (AuNPs) for a rapid and sensitive determination of BPA was designed. To confirm the successful synthesis of CdTFPP, UV/vis, IR, and ¹H NMR were employed. The detection platform CdTFPP/AuNPs/SPCE was prepared using a screen-printed electrode (SPCE), through the deposition of AuNPs and CdTFPP by drop-casting. Under optimized measuring conditions, the developed sensor exhibited two linear ranges from 10⁻¹⁰ to 10⁻⁷ M and 10⁻⁷ to 10⁻² M with a limit of detection of 133 pM. Additionally, the CdTFPP/AuNPs/SPCE/ electrode demonstrated to present an excellent selectivity and reproducibility and to be a promising tool for the development of simple low-cost water quality monitoring system for BPA detection.</p>","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective Electrochemical Sensing of Sunset Yellow in Beverages Based on Synergistic Signal Amplification of HKUST1 and MWCNTs","authors":"Khethiwe Mthiyane,&nbsp;Gloria Ebube Uwaya,&nbsp;Krishna Bisetty","doi":"10.1002/elan.12020","DOIUrl":"https://doi.org/10.1002/elan.12020","url":null,"abstract":"<p>In this study, a highly selective electrooxidation method for sunset yellow (SY) detection in real food samples is presented on the basis of the integration of HKUST1 with multiwalled carbon nanotubes (MWCNTs) doped on a glassy carbon electrode (GCE). Cyclic voltammetry measurements of the HKUST-1-MWCNT-composite electrode revealed enhanced sensitivity for SY with a distinct redox peak potential of approximately 0.6 V, which was attributed to the highly active surface of the electrode. This response was notably more pronounced than those of tartrazine, allura red and amaranth. Electrochemical impedance spectroscopy analysis revealed a low charge transfer resistance (<i>R</i>_ct) for the GCE-HKUST-1-MWCNT electrode, indicating that the integration of HKUST−1 and MWCNTs significantly enhanced the electrical conductivity. In addition, Monte Carlo simulations revealed that a single copper paddle wheel (the secondary building unit of HKUST−1) greatly enhanced the dispersion of the MWCNTs within the matrix. Notably, revealing that the preferred adsorption site of SY was within the inner surface of the MWCNTs, leading to signal amplification. Overall, the sensor achieved limits of detection and limits of quantification of 1.03 and 3.44 μM, respectively, within a defined linear dynamic range. Furthermore, the sensor demonstrated strong practical applicability in real sample analysis, with an excellent recovery rate ranging from 83.11 to 114%. This sensing technique shows great promise for the selective monitoring and detection of synthetic colourants in the food sector.</p>","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elan.12020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective Electrochemical Sensing of Sunset Yellow in Beverages Based on Synergistic Signal Amplification of HKUST1 and MWCNTs","authors":"Khethiwe Mthiyane,&nbsp;Gloria Ebube Uwaya,&nbsp;Krishna Bisetty","doi":"10.1002/elan.12020","DOIUrl":"https://doi.org/10.1002/elan.12020","url":null,"abstract":"<p>In this study, a highly selective electrooxidation method for sunset yellow (SY) detection in real food samples is presented on the basis of the integration of HKUST1 with multiwalled carbon nanotubes (MWCNTs) doped on a glassy carbon electrode (GCE). Cyclic voltammetry measurements of the HKUST-1-MWCNT-composite electrode revealed enhanced sensitivity for SY with a distinct redox peak potential of approximately 0.6 V, which was attributed to the highly active surface of the electrode. This response was notably more pronounced than those of tartrazine, allura red and amaranth. Electrochemical impedance spectroscopy analysis revealed a low charge transfer resistance (<i>R</i>_ct) for the GCE-HKUST-1-MWCNT electrode, indicating that the integration of HKUST−1 and MWCNTs significantly enhanced the electrical conductivity. In addition, Monte Carlo simulations revealed that a single copper paddle wheel (the secondary building unit of HKUST−1) greatly enhanced the dispersion of the MWCNTs within the matrix. Notably, revealing that the preferred adsorption site of SY was within the inner surface of the MWCNTs, leading to signal amplification. Overall, the sensor achieved limits of detection and limits of quantification of 1.03 and 3.44 μM, respectively, within a defined linear dynamic range. Furthermore, the sensor demonstrated strong practical applicability in real sample analysis, with an excellent recovery rate ranging from 83.11 to 114%. This sensing technique shows great promise for the selective monitoring and detection of synthetic colourants in the food sector.</p>","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elan.12020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Additive Manufacturing Electrochemistry: Development of Bismuth Oxide Microparticle Filament for Lead (II) Detection","authors":"Enyioma C. Okpara,&nbsp;Robert D. Crapnell,&nbsp;Elena Bernalte,&nbsp;Olanrewaju B. Wojuola,&nbsp;Craig E. Banks","doi":"10.1002/elan.12019","DOIUrl":"https://doi.org/10.1002/elan.12019","url":null,"abstract":"<p>Accurate, rapid, and cost-effective validation of water quality is essential to ensure that the World Health Organization's (WHO) standards are met and that the United Nations Sustainable Development Goal 6—Clean Water and Sanitation is achieved. To this end, the development of additive-manufactured electrodes using recycled polylactic acid, nanocarbon black, and micro-sized bismuth oxide is reported. These electrodes, that are fabricated using a thermal mixing approach, can be customized to incorporate varying amounts of bismuth oxide (1, 2.5, and 5 wt%) maintaining the integrity of the base polymer. The electrodes developed in this work demonstrate significant potential for the electrochemical detection of lead (II) within water, achieving limits of detection of 0.79, 0.93, and 4.29 μg L⁻¹ (3<i>σ</i>), for the 1, 2.5, and 5 wt% bismuth oxide sensors, respectively. These detection limits are notably below the WHO recommended threshold of 10 μg L⁻¹ for lead in domestic water and even achieve the 2036 European Union targets of 5 μg L⁻¹. The 2.5 wt% bismuth oxide electrodes exhibit excellent reproducibility and specificity, achieving average recovery rates of 98.28% and 100.15% in the analysis of spiked lead (II) samples in deionized and condensed atmospheric water, respectively. This approach is further validated against inductively coupled plasma mass spectroscopy measurements.</p>","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elan.12019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Additive Manufacturing Electrochemistry: Development of Bismuth Oxide Microparticle Filament for Lead (II) Detection","authors":"Enyioma C. Okpara,&nbsp;Robert D. Crapnell,&nbsp;Elena Bernalte,&nbsp;Olanrewaju B. Wojuola,&nbsp;Craig E. Banks","doi":"10.1002/elan.12019","DOIUrl":"https://doi.org/10.1002/elan.12019","url":null,"abstract":"<p>Accurate, rapid, and cost-effective validation of water quality is essential to ensure that the World Health Organization's (WHO) standards are met and that the United Nations Sustainable Development Goal 6—Clean Water and Sanitation is achieved. To this end, the development of additive-manufactured electrodes using recycled polylactic acid, nanocarbon black, and micro-sized bismuth oxide is reported. These electrodes, that are fabricated using a thermal mixing approach, can be customized to incorporate varying amounts of bismuth oxide (1, 2.5, and 5 wt%) maintaining the integrity of the base polymer. The electrodes developed in this work demonstrate significant potential for the electrochemical detection of lead (II) within water, achieving limits of detection of 0.79, 0.93, and 4.29 μg L⁻¹ (3<i>σ</i>), for the 1, 2.5, and 5 wt% bismuth oxide sensors, respectively. These detection limits are notably below the WHO recommended threshold of 10 μg L⁻¹ for lead in domestic water and even achieve the 2036 European Union targets of 5 μg L⁻¹. The 2.5 wt% bismuth oxide electrodes exhibit excellent reproducibility and specificity, achieving average recovery rates of 98.28% and 100.15% in the analysis of spiked lead (II) samples in deionized and condensed atmospheric water, respectively. This approach is further validated against inductively coupled plasma mass spectroscopy measurements.</p>","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elan.12019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Electrochemical Platform Based on Cadmium (II) (fluorophenyl)Porphyrin and Gold Nanoparticles Modified Screen-Printed Electrode Electrode for the Sensitive Detection of Bisphenol A","authors":"Fatma Rejab,&nbsp;Nour Elhouda Dardouri,&nbsp;Ahlem Rouis,&nbsp;Mosaab Echabaane,&nbsp;Habib Nasri,&nbsp;Hamdi Ben Halima,&nbsp;Nicole Jaffrezic-Renault","doi":"10.1002/elan.12017","DOIUrl":"https://doi.org/10.1002/elan.12017","url":null,"abstract":"<p>Bisphenol A (BPA) is categorized as an endocrine-disrupting substance; it is a risky substance to both environmental and human health, necessitating monitoring. In this present study, a novel electrochemical sensing platform based on Cd metalloporphyrin ([(5,10,15,20- tetrakis(p-flurophenyl) porphyrinato] cadmium (II) [CdTFPP]) and gold nanoparticles (AuNPs) for a rapid and sensitive determination of BPA was designed. To confirm the successful synthesis of CdTFPP, UV/vis, IR, and ¹H NMR were employed. The detection platform CdTFPP/AuNPs/SPCE was prepared using a screen-printed electrode (SPCE), through the deposition of AuNPs and CdTFPP by drop-casting. Under optimized measuring conditions, the developed sensor exhibited two linear ranges from 10⁻¹⁰ to 10⁻⁷ M and 10⁻⁷ to 10⁻² M with a limit of detection of 133 pM. Additionally, the CdTFPP/AuNPs/SPCE/ electrode demonstrated to present an excellent selectivity and reproducibility and to be a promising tool for the development of simple low-cost water quality monitoring system for BPA detection.</p>","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design of a Peroxidase-Based Gas Diffusion Electrode for Bioelectroenzymatic Applications","authors":"Vladyslav Mishyn,&nbsp;Nunzio Giorgio G. Carducci,&nbsp;David P. Hickey,&nbsp;Sofiene Abdellaoui","doi":"10.1002/elan.12015","DOIUrl":"https://doi.org/10.1002/elan.12015","url":null,"abstract":"<p>We report here the design and application of an all-in-one gas diffusion electrode (GDE) combined with surface immobilized horseradish peroxidase (HRP) capable of <i>in situ</i> generation of hydrogen peroxide (H₂O₂) from air and simultaneous oxidation of a model substrate (ABTS) within a single-cell electrochemical reactor. Carboxyl-functionalized multiwalled carbon nanotubes (MWCNT-COOH) were employed as an electrocatalyst for oxygen reduction reaction producing 719 ± 97 µM h⁻¹ H₂O₂ at −0.2 V (<i>vs</i> Ag/AgCl) as an electron acceptor for HRP. We investigated two immobilization strategies to obtain HRP-modified biocathodes using covalent amide conjugation between primary amine groups of HRP and carboxyl groups of MWCNT-COOH (GDE/MWCNT-COOH/HRP) and entrapment into a cross-linked pyrene-modified linear poly(ethylenimine) matrix (GDE/MWCNT-COOH/Py-LPEI/HRP). Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) were used to characterize such surface modifications. The apparent catalytic activity achieved by HRP-modified biocathodes <i>via</i> either covalent conjugation or entrapment into a polymer film was 311 ± 31 U mg⁻¹ and 174 ± 17 U mg⁻¹, respectively, as compared to the activity of freely diffusing 188 ± 23 U mg⁻¹. The interfaces were reused showing 55% and 82% residual activity after 5 consecutive cycles for GDE/MWCNT-COOH/HRP and GDE/MWCNT-COOH/Py-LPEI/HRP, respectively. Our findings illustrate prospects for integrating GDE and surface-bound peroxidases for H₂O₂-dependent electroenzymatic reactions, offering a promising platform for diverse applications in bioelectrosynthesis.</p>","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143111886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A 3D Layered ASPC-CoS@Cos2 Composite Electrode Film via Simple Hydrothermal Method for High-Performance Supercapacitors","authors":"Weihong Cai,&nbsp;Zhentao Liu,&nbsp;Weijing Mo,&nbsp;Yao Liang,&nbsp;Qixin Cheng,&nbsp;Huixin Jin,&nbsp;Meilong Wang","doi":"10.1002/elan.12014","DOIUrl":"https://doi.org/10.1002/elan.12014","url":null,"abstract":"<p>Constructing suitable nanostructures can provide a large specific surface area for storing charge. A 3D layered CoS@CoS₂ composite electrode film grown on activated shaddock peel-derived carbon (ASPC-CoS@CoS₂) was prepared by a simple hydrothermal method in this work. The specific capacitance of the ASPC-CoS@CoS₂ is 1160 F g⁻¹ at 1.0 A g⁻¹. When assembled into a supercapacitor (ASC: ASPC-CoS@CoS₂//ASPC), the ASC device achieved an energy density of 40.4 Wh kg⁻¹ at 364.5 W kg⁻¹. This enhancement in performance can be ascribed to the unique layered porous structure.</p>","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143111887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design of a Peroxidase-Based Gas Diffusion Electrode for Bioelectroenzymatic Applications","authors":"Vladyslav Mishyn,&nbsp;Nunzio Giorgio G. Carducci,&nbsp;David P. Hickey,&nbsp;Sofiene Abdellaoui","doi":"10.1002/elan.12015","DOIUrl":"https://doi.org/10.1002/elan.12015","url":null,"abstract":"<p>We report here the design and application of an all-in-one gas diffusion electrode (GDE) combined with surface immobilized horseradish peroxidase (HRP) capable of <i>in situ</i> generation of hydrogen peroxide (H₂O₂) from air and simultaneous oxidation of a model substrate (ABTS) within a single-cell electrochemical reactor. Carboxyl-functionalized multiwalled carbon nanotubes (MWCNT-COOH) were employed as an electrocatalyst for oxygen reduction reaction producing 719 ± 97 µM h⁻¹ H₂O₂ at −0.2 V (<i>vs</i> Ag/AgCl) as an electron acceptor for HRP. We investigated two immobilization strategies to obtain HRP-modified biocathodes using covalent amide conjugation between primary amine groups of HRP and carboxyl groups of MWCNT-COOH (GDE/MWCNT-COOH/HRP) and entrapment into a cross-linked pyrene-modified linear poly(ethylenimine) matrix (GDE/MWCNT-COOH/Py-LPEI/HRP). Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) were used to characterize such surface modifications. The apparent catalytic activity achieved by HRP-modified biocathodes <i>via</i> either covalent conjugation or entrapment into a polymer film was 311 ± 31 U mg⁻¹ and 174 ± 17 U mg⁻¹, respectively, as compared to the activity of freely diffusing 188 ± 23 U mg⁻¹. The interfaces were reused showing 55% and 82% residual activity after 5 consecutive cycles for GDE/MWCNT-COOH/HRP and GDE/MWCNT-COOH/Py-LPEI/HRP, respectively. Our findings illustrate prospects for integrating GDE and surface-bound peroxidases for H₂O₂-dependent electroenzymatic reactions, offering a promising platform for diverse applications in bioelectrosynthesis.</p>","PeriodicalId":162,"journal":{"name":"Electroanalysis","volume":"37 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143112093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}