Journal of Liquid Chromatography & Related Technologies最新文献

{"title":"Characterization of quality differences of Ophiopogonis Radix from different origins by TLC, HPLC, UHPLC-MS and multivariate statistical analyses","authors":"Ling Jiang, Y. Qiu, Zhuliang Chen, Lunbo Luo, Hongxia Tang, Xudong Zhou, Hanwen Yuan, Wen Wang, Ping Liu","doi":"10.1080/10826076.2022.2159977","DOIUrl":"https://doi.org/10.1080/10826076.2022.2159977","url":null,"abstract":"Abstract Ophiopogonis Radix is normally used as a traditional medicine and healthy food in China. Thin-layer chromatography (TLC), total saponin content analysis, high-performance liquid chromatographic (HPLC) fingerprinting, ultra-high-performance liquid chromatographic (UHPLC) tandem mass (MS) spectra and multivariate statistical analyses were used to analyze differences in quality of Ophiopogonis Radix from three main producing areas in China. The TLC spots of samples obtained from Zhejiang were clearer and darker than samples from Sichuan and Shandong. Additionally, HPLC fingerprinting analysis of 14 common peaks of the Ophiopogonis Radix samples indicated that the similarities of the 15 batches of samples were between 0.94 and 0.99. Finally, UPLC-Orbitrap MS analysis was used to identify the 13 common peaks of Ophiopogonis Radix. These results showed that Ophiopogonis Radix obtained from different regions can be distinguished well using a combination of analytical techniques, based on differences in their chemical components. The combination of these techniques allows comprehensive and reliable quality evaluation of Ophiopogonis Radix. This study provides references for determining the quality of Ophiopogonis Radix obtained from different regions, which will guide its use as medicine or food. GRAPHICAL ABSTRACT","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":"45 1","pages":"120 - 129"},"PeriodicalIF":1.3,"publicationDate":"2022-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47091062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging trends in extraction and analytical techniques for bromelain","authors":"D. Maheshwari, J. Shah, Darshil Shah, Paresh K. Patel, Yash Raj Singh","doi":"10.1080/10826076.2022.2161573","DOIUrl":"https://doi.org/10.1080/10826076.2022.2161573","url":null,"abstract":"Abstract Bromelain, a proteolytic enzyme isolated from the stem and fruit of Ananas comosus has gained tremendous importance as anti-inflammatory, anticancer, and fibrinolytic agent in the clinical field. Recent anti-cancer and anti-thrombolytic activity of Bromelain has opened new areas of research in the field of safety and efficacy. Bromelain is widely used since time immemorial alone and in combination with other drugs in the treatment of Rheumatoid Arthritis. The review aims to evaluate various extraction and analytical techniques employed for the assay of Bromelain in complex enzymatic mixtures which involves the use of diverse derivatizing agents with variable sensitivity and accuracy. The review focuses on homogenous and heterogenous techniques used for the estimation of Bromelain in the crude extract. This review also emphasizes on separation novel techniques, such as ion-exchange chromatography for isolation and purification of Bromelain supported by Gel Chromatography. This review also aims to provide deep insight into recent reverse micellar techniques employed as an effective tool for the purification of Bromelain. An overview of hyphenated bioanalytical techniques, such as LC-MS/MS for analysis of Bromelain in Plasma is also inculcated in the study. The techniques presented in the study provide diversified tools employed for the analysis of Bromelain in crude mixtures of complex enzymes of clinical significance. GRAPHICAL ABSTRACT","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":"45 1","pages":"107 - 119"},"PeriodicalIF":1.3,"publicationDate":"2022-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49018966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the major degradation products of the praziquantel API by mass spectrometry: Development and validation of a stability-indicating reversed phase UPLC method","authors":"Ajay Kumar, R. Chalannavar","doi":"10.1080/10826076.2022.2163253","DOIUrl":"https://doi.org/10.1080/10826076.2022.2163253","url":null,"abstract":"Abstract Praziquantel was subjected to forced degradation studies under various conditions of hydrolysis (acidic, alkaline, and neutral/water), oxidation, photolysis, and thermal as prescribed by International Conference on Harmonization guideline Q1A (R2). A short and simple reversed phase UHPLC method was developed for the Praziquantel API. The method was developed on a Shimadzu, Shimpack Velox, SP-C18, 100 × 2.1 mm, 1.8 µm column. The isocratic mobile phase was a blend of water and acetonitrile in the ratio of 70:30 (v/v), respectively. The flow rate of the mobile phase was 0.5 mL/min. The developed method was validated using ICH guidelines. The parameters considered for method validation were solution stability, specificity, DL/QL, linearity, accuracy, precision, and robustness. The drug showed significant degradation in acidic and alkaline conditions while slight degradation was observed in water and oxidative conditions. The drug was found to be stable in photolytic and thermal conditions. The characterization of four major degradation products (DP1, DP2, DP3, and DP4) was done with LC–Q-TOF-MS/MS in combination with accurate mass measurements. The most probable mechanisms for the formation of DPs have been proposed on the basis fragmentation pattern. Graphical Abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":"45 1","pages":"130 - 142"},"PeriodicalIF":1.3,"publicationDate":"2022-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45382450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study on flavonoids from Tibetan medicinal plants Saussurea species using HPLC-DAD-ESI-MS","authors":"Nianhua Jing, Junyou Shi, Jiang Hu, Zhiwei Sun","doi":"10.1080/10826076.2023.2165096","DOIUrl":"https://doi.org/10.1080/10826076.2023.2165096","url":null,"abstract":"Abstract Three kinds of “Snowlotus” including Saussurea involucrate, Saussurea medusa and Saussurea laniceps had been prescribed as folk medicine interchangeably in Tibet and Uygur, China. To verify the feasibility of this phenomenon, a HPLC-DAD-ESI-MS method was developed in this study to determine the flavonoid compounds from three Saussurea species qualitatively and quantitatively. The LODs and LOQs ranged from 0.0263–0.1172 µg/mL and 0.0862–0.3867 µg/mL, respectively. The relative standard deviation (RSD × 100%) of the validation for the instrument precision was below 2.03%, while the RSD × 100% values for the method precision were below 5.73%, respectively. The recoveries ranged from 92.98–101.79%. These results further demonstrated that this method was precise and practical for determining flavonoid compounds from three Saussurea species. In this study, 14 kinds of constituents were simultaneously identified according to the ESI-MS data and previous studies. The contents of total flavonoids and eight components were determined with spectrophotometry and HPLC-DAD methods, respectively. The results demonstrated that the total flavonoids in S. involucrate had the hightest value and there were some differences in the content of eight flavonoids. For S. involucrate, a proportion of rutin, luteolin-7-O-glucoside, and hispidulin were detected. For S. laniceps, Arctiin, apigenin, and arctigenin were the major flavonoid compounds. For S. medusa, seven types of compounds were detected and arctiin presented the highest value, whereas apigenlin exhibited the lowest value. This comparative study may promote the differentiation in use of three kinds of snowlotus. Graphical abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":"45 1","pages":"156 - 163"},"PeriodicalIF":1.3,"publicationDate":"2022-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45050185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of a recombinase-aided amplification assay using betaine and pullulan.","authors":"Jinrong Wang, Guowei Song, Yue Ming, Jing Pan, Ruiqing Zhang, Guohao Fan, Xinxin Shen, Xuejun Ma, Lixin Li","doi":"10.1016/j.imj.2022.06.002","DOIUrl":"10.1016/j.imj.2022.06.002","url":null,"abstract":"<p><strong>Background: </strong>Nucleic acid amplification enhancers suitable for use in a recombinase-aided amplification (RAA) assay were studied for the first time, and amplification of a long-fragment (509 bp) was initially explored.</p><p><strong>Methods: </strong>Using recombinant plasmids and clinical samples, RAA fluorescence and basic methods were used to evaluate the efficacy. The fluorescence method was evaluated by threshold time and fluorescence value, and the basic method was characterized by 2% agarose gel electrophoresis.</p><p><strong>Results: </strong>Taking a previously established RAA assay for HPV18 as an example, we demonstrated that the addition of 0.2 M, 0.4 M, and 0.6 M betaine and 10% pullulan could enhance the RAA. The new RAA assays with betaine and pullulan were named B-RAA and P-RAA, respectively. Using the B-RAA and P-RAA fluorescence methods, the threshold time values could be shortened by 1.72-2.32 minutes and 2.60 minutes, respectively, and the fluorescence values could be enhanced by 8847.25-9094.37 mv and 5250 mv, respectively. Using the basic method, the sensitivity could be increased 10-fold. We successfully amplified a long-fragment of 509 bp using a P-RAA assay with a sensitivity of 10² copies/µL (compared with 10³ copies/µL in the RAA assay).</p><p><strong>Conclusions: </strong>Thus, we concluded that betaine and pullulan are effective additives to enhance the sensitivity of RAA assays.</p>","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":"12 1","pages":"73-80"},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10699723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81190856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Quantitative Model of Sporadic Axonal Degeneration in the <i>Drosophila</i> Visual System.","authors":"Mélisande Richard, Karolína Doubková, Yohei Nitta, Hiroki Kawai, Atsushi Sugie, Gaia Tavosanis","doi":"10.1523/JNEUROSCI.2115-21.2022","DOIUrl":"10.1523/JNEUROSCI.2115-21.2022","url":null,"abstract":"<p><p>In human neurodegenerative diseases, neurons undergo axonal degeneration months to years before they die. Here, we developed a system modeling early degenerative events in <i>Drosophila</i> adult photoreceptor cells. Thanks to the stereotypy of their axonal projections, this system delivers quantitative data on sporadic and progressive axonal degeneration of photoreceptor cells. Using this method, we show that exposure of adult female flies to a constant light stimulation for several days overcomes the intrinsic resilience of R7 photoreceptors and leads to progressive axonal degeneration. This was not associated with apoptosis. We furthermore provide evidence that loss of synaptic integrity between R7 and a postsynaptic partner preceded axonal degeneration, thus recapitulating features of human neurodegenerative diseases. Finally, our experiments uncovered a role of postsynaptic partners of R7 to initiate degeneration, suggesting that postsynaptic cells signal back to the photoreceptor to maintain axonal structure. This model can be used to dissect cellular and circuit mechanisms involved in the early events of axonal degeneration, allowing for a better understanding of how neurons cope with stress and lose their resilience capacities.<b>SIGNIFICANCE STATEMENT</b> Neurons can be active and functional for several years. In the course of aging and in disease conditions leading to neurodegeneration, subsets of neurons lose their resilience and start dying. What initiates this turning point at the cellular level is not clear. Here, we developed a model allowing to systematically describe this phase. The loss of synapses and axons represents an early and functionally relevant event toward degeneration. Using the ordered distribution of <i>Drosophila</i> photoreceptor axon terminals, we assembled a system to study sporadic initiation of axon loss and delineated a role for non-cell-autonomous activity regulation in the initiation of axon degeneration. This work will help shed light on key steps in the etiology of nonfamilial cases of neurodegenerative diseases.</p>","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":"6 1","pages":"4937-4952"},"PeriodicalIF":0.0,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9188428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81171483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating caregiver stress in craniosynostosis patients.","authors":"Alan R Tang, Jeffrey W Chen, Georgina E Sellyn, Heidi Chen, Shilin Zhao, Stephen R Gannon, Chevis N Shannon, Christopher M Bonfield","doi":"10.3171/2022.4.PEDS21596","DOIUrl":"10.3171/2022.4.PEDS21596","url":null,"abstract":"<p><strong>Objective: </strong>Caregiver stress from a child's diagnosis can impact a caregiver's ability to participate in treatment decisions, comply, and manage long-term illness. The aim of this study was to compare caregiver stress in children with craniosynostosis at diagnosis and postoperatively.</p><p><strong>Methods: </strong>This prospective study included caregivers of pediatric patients with craniosynostosis receiving operative intervention. Demographics and Parenting Stress Index, Short Form (PSI-SF) and Pediatric Inventory for Parents (PIP) surveys at baseline (preoperatively) and 3 and 6 months postoperatively were completed. PSI-SF scores between 15 and 80 are considered normal, with > 85 being clinically significant and requiring follow-up. Higher PIP scores represent increased frequency and difficulty of stressful events due to the child's illness. Pairwise comparisons were performed using the Wilcoxon signed-rank test. Multivariate analysis was performed to assess for PSI-SF and PIP predictors.</p><p><strong>Results: </strong>Of 106 caregivers (84% Caucasian), there were 62 mothers and 40 fathers. There were 68 and 45 responses at 3 and 6 months postoperatively, respectively. Regarding the baseline group, more than 80% were between 20 and 40 years of age and 58% had less than 2 years of college education. The median household income fell in the $45,001-$60,000 bracket. There was no significant difference between median baseline PSI-SF score (65, IQR 51-80) and those at 3 months (p = 0.45) and 6 months (p = 0.82) postoperatively. Both median PIP frequency (89 vs 74, p < 0.01) and difficulty (79 vs 71, p < 0.01) scores were lower at 3 months, although no significant difference was observed at 6 months (frequency: 95 vs 91, p = 0.67; difficulty: 82 vs 80, p = 0.34). Female sex, uninsured status, and open surgery type were all risk factors for higher parental stress.</p><p><strong>Conclusions: </strong>Stress levels ranged from normal to clinically significant in the caregivers, with sex, uninsured status, and open repair predicting higher stress. Stress decreased at 3 months postoperatively before increasing at 6 months. Intervention targeting caregiver stress should be explored to maintain lower stress observed at 3 months after surgery.</p>","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":"28 1","pages":"224-231"},"PeriodicalIF":1.9,"publicationDate":"2022-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82348851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An overview of liquid chromatographic methods for analyzing new generation anti-epileptic drugs","authors":"Mohammed AlSaeedy, Ahmed Hasan, Arwa Al-Adhreai, Ali Alrabie, Hafsah Qaba, Abdulrahman Mashrah, Elif Mine Öncü-Kaya","doi":"10.1080/10826076.2022.2134146","DOIUrl":"https://doi.org/10.1080/10826076.2022.2134146","url":null,"abstract":"Abstract Pregabalin, Lacosamide, Rufinamide, Perampanel, Eslicarbazepine acetate, Brivaracetam, Cannabidiol, Stiripentol, Everolimus, Cenobamate, and Fenfluramine are drugs of the third generation of antiepileptic drugs registered by International Coalition of Medicine Regulatory Authorities between 2000 and 2021. There are 213 peer-reviewed papers on antiepileptic drug analysis in the literature over the last three decades (1990–2022) which were reviewed in this article to provide an extensive overview of liquid chromatography techniques used to determine antiepileptic drugs. These details were discussed on the basis of a matrix: additives that are used to prepare a dosage form, sample preparation technique, column, limits of detection and quantification, types of elution in chromatography (isocratic or gradient), and detector. This review article will provide vital information to the scientific community in order to identify the most appropriate approach for antiepileptic drug analysis and determination. Graphical Abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":"45 1","pages":"66 - 83"},"PeriodicalIF":1.3,"publicationDate":"2022-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47489979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Separation of acetylferrocene from synthetic mixtures by countercurrent chromatography","authors":"Xiao‐yong Lv, Haibo You, Huiyun Qiu, Mengyi Wen, Chuncui Zheng, Shengqiang Tong","doi":"10.1080/10826076.2022.2143798","DOIUrl":"https://doi.org/10.1080/10826076.2022.2143798","url":null,"abstract":"Abstract The main product of acetylation of ferrocene was successfully separated and purified with high purity from synthetic mixtures by countercurrent chromatography using a selected biphasic solvent system. According to distribution performance of main product and reactant, a two-phase solvent system was selected by thin layer chromatography, in which the partition coefficient for each component was determined by HPLC. Then a two-phase solvent system consisting of petroleum ether/ethyl acetate/methanol/water (6:4:6:4, v/v) was selected. After acetylation reaction, 20 mg of synthetic mixture was subjected to countercurrent chromatography, yielding 2.1 mg of ferrocene and 8.7 mg of acetyl ferrocene with 96.6% and 97.4% purity. Results indicated that countercurrent chromatography is a very efficient method for separation and purification of target product from synthetic mixtures. GRAPHICAL ABSTRACT","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":"45 1","pages":"94 - 99"},"PeriodicalIF":1.3,"publicationDate":"2022-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47783617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Countercurrent chromatography: separation principle, mechanical design, development trends, and applications","authors":"Zhicong Liu, Yongjie Zhao, Huihui Peng, Tiexuan Luo, Yang Liu","doi":"10.1080/10826076.2022.2117190","DOIUrl":"https://doi.org/10.1080/10826076.2022.2117190","url":null,"abstract":"Abstract Countercurrent chromatography (CCC) is the separation technique based on the different partition coefficients of the sample between two immiscible solvents, which is a liquid-liquid partition without solid support in the column. In this paper, Firstly, the mechanic’s equilibrium principle of CCC is described, which includes hydrostatic equilibrium system (HSES), hydrodynamic equilibrium system (HDES), unidirectional hydrodynamic equilibrium phenomenon, etc. The separation principle of the CCC is also explained. Secondly, the mechanical structure and design of parallel-axis CCC, cross-axis CCC, oblique-axis CCC, as well as some CCC derived from the three are illustrated respectively. Finally, the existing problems for the development of CCC have been exposed, and the trend of the future development direction and applications of CCC are discussed. Graphical Abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":"45 1","pages":"51 - 65"},"PeriodicalIF":1.3,"publicationDate":"2022-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46058462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}