Journal of Liquid Chromatography & Related Technologies最新文献_第10页

Identification and characterization of novel hydrolytic degradation products of netarsudil by LC-Q-TOF-MS/MS: In silico toxicity prediction 用LC-Q-TOF-MS/MS鉴定奈沙地尔新型水解降解产物及其在硅毒性预测中的应用

IF 1.3 4区化学

Journal of Liquid Chromatography & Related Technologies Pub Date : 2022-02-25 DOI: 10.1080/10826076.2022.2089999

Surapuraju Pavan Kumar Raju, Juturu Ravindra Reddy

{"title":"Identification and characterization of novel hydrolytic degradation products of netarsudil by LC-Q-TOF-MS/MS: In silico toxicity prediction","authors":"Surapuraju Pavan Kumar Raju, Juturu Ravindra Reddy","doi":"10.1080/10826076.2022.2089999","DOIUrl":"https://doi.org/10.1080/10826076.2022.2089999","url":null,"abstract":"Abstract A forced degradation study on novel glaucoma drug netarsudil was performed as per ICH and WHO guidelines. All the degradation products were separated from the drug on a C-18 column utilizing ammonium acetate buffer (10 mM, pH 4.90) as a mobile phase-A and acetonitrile: methanol (70:30) v/v as mobile phase-B, by employing gradient elution mode. The injection volume was 10 µL with a 0.8 mL/min flow rate. The detection wavelength was 245 nm and the study was executed at a constant column temperature of 40 °C. Initially, the mass fragmentation pathway of the drug was postulated followed by characterization of degradation products from LC-MS-TOF/MS data. This was followed by LC-MS/TOF studies on the degradation products. Significant degradation was witnessed in alkaline and acidic conditions whereas no signification decline in the drug was observed in other decomposition environments. A total of four degradation products were formed and characterized with the aid of high-resolution mass spectrometry. The mechanistic pathway of formation of all DPs from netarsudil is established. In addition, comparative toxicity properties of the drug and degradation products were established using the online web server Pro Tox II. Graphical Abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2022-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44965313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Indexation of mixed mode adsorbents using low temperature nitrogen adsorption and Silica-C18 surface energy scale 低温氮吸附和Silica-C18表面能标度对混合模式吸附剂的指数化

IF 1.3 4区化学

Journal of Liquid Chromatography & Related Technologies Pub Date : 2021-12-14 DOI: 10.1080/10826076.2022.2110117

Dinah Lee, Y. Kazakevich

{"title":"Indexation of mixed mode adsorbents using low temperature nitrogen adsorption and Silica-C18 surface energy scale","authors":"Dinah Lee, Y. Kazakevich","doi":"10.1080/10826076.2022.2110117","DOIUrl":"https://doi.org/10.1080/10826076.2022.2110117","url":null,"abstract":"Abstract Mixed-mode chromatography is becoming increasingly popular in pharmaceutical and biopharmaceutical applications due to its unique selectivity and retention of a variety of compounds. Mixed-mode chromatography is a chromatographic method in which solutes interact with stationary phase through more than one interaction mode or mechanism. It is because of these complex interactions, however, that it is difficult to predict chromatographic behavior of analytes on mixed-mode columns. Characterization of different mixed-mode columns on the basis of surface energy characteristics has not been explored. In this study, different ratios of porous silica (Axia Luna 5 µm Silica – Phenomenex), and C18 (Axia Luna 5 µm C18 – Phenomenex), single mode adsorbents were blended in various ratios and analyzed by Low Temperature Nitrogen Adsorption (LTNA). A variety of commercially available mixed mode columns were also unpacked and the materials were analyzed by LTNA. Adsorption isotherms, surface area, and BET constants were obtained for all the blended materials in various ratios and the mixed-mode materials. A linear relationship between the BET C-constant and the blend ratio of silica has been observed. The results from the commercial mixed mode packing material showed similar surface energy characteristics to single mode C18 materials. This data suggests that the BET C-constant maybe a viable parameter that can be used to characterize mixed-mode columns on the basis of surface energy. Utilizing this surface energy scale in conjunction with the study of retention behaviors on mixed-mode material could lead to improvements in the understanding of method development on mixed-mode columns, allow the comparison of commercialized mixed-mode columns, and even predict retention behavior of an analyte in mixed-mode columns. Graphical Abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2021-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49431578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Separation optimization and quantitative analysis of phytoestrogens employing reverse-phase high-performance liquid chromatography with UV-VIS detection 紫外可见反相高效液相色谱法优化植物雌激素分离及定量分析

IF 1.3 4区化学

Journal of Liquid Chromatography & Related Technologies Pub Date : 2021-12-14 DOI: 10.1080/10826076.2022.2110118

Michael Payette, Marta R. M. Lima, W. M. Coleman, M. Ashraf-Khorassani

{"title":"Separation optimization and quantitative analysis of phytoestrogens employing reverse-phase high-performance liquid chromatography with UV-VIS detection","authors":"Michael Payette, Marta R. M. Lima, W. M. Coleman, M. Ashraf-Khorassani","doi":"10.1080/10826076.2022.2110118","DOIUrl":"https://doi.org/10.1080/10826076.2022.2110118","url":null,"abstract":"Abstract The effect of different acidic modifiers on the separation of seven selected phytoestrogens by reverse-phase high-performance liquid chromatography has been described. Variation in the hydrocarbon chain length of organic acids as represented by acetic acid, propanoic acid, iso-butyric acid, and n-butyric acid as additives to the mobile phase had minimal effect on the separation of phytoestrogens but notably reduced sample turnaround times relative to the turnaround time linked with formic acid. Two different C18 columns were evaluated in attempts of optimize the phytoestrogen separations. Formic acid as a modifier in the mobile phase and an Atlantis C18 column provided the best overall separation at optimum gradient conditions. A higher acid concentration in the mobile phase did not improve the phytoestrogen separation. Higher column temperature (40 °C) provided an improved separation. Finally, the optimized method was used to quantify different phytoestrogens in selected different botanical samples. For this purpose, both internal and external standard methods were compared to obtain the quantitative results. Graphical Abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2021-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46869503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

In memoriam: Professor Harold M. McNair 纪念：哈罗德·麦克奈尔教授

IF 1.3 4区化学

Journal of Liquid Chromatography & Related Technologies Pub Date : 2021-12-14 DOI: 10.1080/10826076.2022.2110119

N. Snow

引用次数: 0

Current prospects on nano liquid chromatography coupled to electron ionization mass spectrometry (nanoLC-EI-MS) 纳米液相色谱-电子电离质谱联用技术的发展前景

IF 1.3 4区化学

Journal of Liquid Chromatography & Related Technologies Pub Date : 2021-12-14 DOI: 10.1080/10826076.2022.2110114

D. A. Vargas Medina, Natalia Gabrielly Pereira dos Santos, E. Maciel, F. Lanças

{"title":"Current prospects on nano liquid chromatography coupled to electron ionization mass spectrometry (nanoLC-EI-MS)","authors":"D. A. Vargas Medina, Natalia Gabrielly Pereira dos Santos, E. Maciel, F. Lanças","doi":"10.1080/10826076.2022.2110114","DOIUrl":"https://doi.org/10.1080/10826076.2022.2110114","url":null,"abstract":"Abstract NanoLC consolidates as powerful analytical technology liable to provide improved chromatographic performance and superior detection capabilities. Significant instrumental advances have been accomplished in the last 20 years to make nanoLC instruments more robust, accurate, and amenable for routine analysis. Similarly, new stationary phases and nanoLC formats provide new opportunities for separations at the miniaturized scale. Besides, nanoLC makes the hyphenation with EI-MS feasible, and a series of interfaces and interesting applications have been reported in the last years. Ei-MS offers unparallel identification capabilities, and its coupling with nanoLC has the potential to revolutionize the Performance of LC-MS untargeted studies. This manuscript makes a historical journey through the development of the nanoLC and its hyphenation with the EI-MS, exploring the more outstanding advances in that technology, discussing its scope, and identifying its limitations. We intend to give the reader a current overview of the state of the art of nanoLC and point out perspectives and future lines of advancement and improvement for new research in this area. Graphical Abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2021-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43236127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Determination of selected herbicides employed in sugarcane crops by disposable pipette tip (DPX) extraction followed by LC-MS/MS and GC-MS/MS 一次性移液管尖端(DPX)萃取- LC-MS/MS和GC-MS/MS联合测定甘蔗作物中选定除草剂

IF 1.3 4区化学

Journal of Liquid Chromatography & Related Technologies Pub Date : 2021-12-14 DOI: 10.1080/10826076.2022.2110115

Thaís Camarotto Oliveira, F. Lanças

{"title":"Determination of selected herbicides employed in sugarcane crops by disposable pipette tip (DPX) extraction followed by LC-MS/MS and GC-MS/MS","authors":"Thaís Camarotto Oliveira, F. Lanças","doi":"10.1080/10826076.2022.2110115","DOIUrl":"https://doi.org/10.1080/10826076.2022.2110115","url":null,"abstract":"Abstract A disposable pipette tip extraction (DPX) method followed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was developed and validated to determine eleven herbicides employed in sugarcane crops. After improving conditions, the DPX method consists of conditioning a C18 tip with 0.3 mL of methanol, followed by the same volume of water and 0.15 mL of the sample. After completing the sorption interaction, the sample was dispensed, and elution was made with 0.15 mL of methanol before collecting the extract for injection. The total extraction time was 4 min, and recoveries ranged from 59.1 to 76.5%. DPX method was compared to cartridge solid-phase extraction, which presented recoveries between 30.4 and 108.6%. SPE consumed four times more solvent, total extraction time is five times longer than DPX, and cartridges are more expensive than the DPX tips. DPX performance was also evaluated by gas chromatography coupled with tandem mass spectrometry (GC-MS/MS). The recoveries were similar to LC-MS/MS. Furthermore, the DPX-C18-LC-MS/MS method was applied to analyze sugarcane-derived juice samples. Results indicate that the method can determine the herbicides in actual samples and other sugarcane matrices. Graphical Abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2021-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48423968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Streamlined stationary phase selection facilitated by a “sample-plug retention test” in supercritical fluid extraction-supercritical fluid chromatography-mass spectroscopy (SFE-SFC-MS) method development for on-line extraction of anabolic agents 在超临界流体萃取-超临界流体色谱-质谱(SFE-SFC-MS)在线提取合成代谢剂的方法开发中，通过“样品塞保留试验”简化了固定相选择

IF 1.3 4区化学

Journal of Liquid Chromatography & Related Technologies Pub Date : 2021-12-14 DOI: 10.1080/10826076.2022.2098319

B. Berger, A. Wicker, E. Preuss, Yuka Fujito, W. Hedgepeth, Masayuki Nishimura, K. Schug

{"title":"Streamlined stationary phase selection facilitated by a “sample-plug retention test” in supercritical fluid extraction-supercritical fluid chromatography-mass spectroscopy (SFE-SFC-MS) method development for on-line extraction of anabolic agents","authors":"B. Berger, A. Wicker, E. Preuss, Yuka Fujito, W. Hedgepeth, Masayuki Nishimura, K. Schug","doi":"10.1080/10826076.2022.2098319","DOIUrl":"https://doi.org/10.1080/10826076.2022.2098319","url":null,"abstract":"Abstract A novel method development (MD) approach was established to aid column selection for online supercritical fluid extraction-supercritical fluid chromatography-mass spectrometry (SFE-SFC-MS). An adapted stationary phase (SP) selection procedure was used to expand traditional SFC-MS screening by inclusion of an “Analyte-Plug Retention Test.” This test enabled an early evaluation of “trapping” capability for a plug of targeted analytes on the SP. Using an SFE-simulation method, the on-line trapping of an SFE-based extract-plug was mimicked by trapping an SFC-based injection-plug under instrument conditions similar to an actual on-line extraction. Thirteen SPs were screened using the adapted MD procedure as part of the SFC-separation optimization in the development of an on-line SFE-SFC-MS extraction method for anabolic agents (AAS) in anti-doping testing. A cyano SP was chosen for further development, based on chromatographic separation potential and high plug-retention potential for targeted analytes. The chosen column was used in “proof-of-concept” on-line extractions using a generic SFE-SFC-MS method. Successful extract-plug trapping was demonstrated. Retention times and peak areas (4–20%RSD) were reproducible for targeted AAS. Successful first round SFE-SFC-MS SP selection was demonstrated to circumvent tedious re-optimization in future development stages and streamlining of the hyphenated SFE-SFC-MS MD process. Graphical Abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2021-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48284273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Extraction of glucocorticoids from water into acetonitrile using polyol induced extraction with ultra performance liquid chromatography and triple quadrupole mass spectrometry (PIE-UPLC-MS-MS) 多元醇诱导萃取-超高效液相色谱-三重四极杆质谱法(PIE-UPLC-MS-MS)从水中提取糖皮质激素

IF 1.3 4区化学

Journal of Liquid Chromatography & Related Technologies Pub Date : 2021-12-14 DOI: 10.1080/10826076.2022.2110116

Shipra Patel, N. Snow

{"title":"Extraction of glucocorticoids from water into acetonitrile using polyol induced extraction with ultra performance liquid chromatography and triple quadrupole mass spectrometry (PIE-UPLC-MS-MS)","authors":"Shipra Patel, N. Snow","doi":"10.1080/10826076.2022.2110116","DOIUrl":"https://doi.org/10.1080/10826076.2022.2110116","url":null,"abstract":"Abstract Polyol-induced Extraction (PIE) was used for the extraction and UPLC-MS-MS was used for the analysis of glucocorticoids in water. The focus of this work was to demonstrate PIE as an effective technique that allows extraction of solutes between miscible solutes using a phase separating agent, with an example analysis of glucocorticoids. In this work glycerol is used as the mass separating agent in the extraction of glucocorticoids from water into acetonitrile. Eight glucocorticoids were extracted by PIE and analyzed by UPLC-MS-MS. Extraction conditions were optimized, and extraction results were compared to non-extracted glucocorticoid standards and to literature methods. Extraction was optimized at 0 °C, in 10 mL of a 1:1 ACN/Water mixture 2 mL of glycerol added as phase separating agent. Calibration curves showed overall correlation of r 2 > 0.99. Precent relative standard deviation below 4%was obtained for all analytes. Limits of detection were obtained between 3–4 ng/mL by the IUPAC method and 40–200 ng/mL by a propagation of errors method. PIE is demonstrated to be a simple and quick method for extracting glucocorticoids from water into a miscible polar solvent, acetonitrile. Graphical Abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2021-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45129835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a rapid LC-MS/MS method for the simultaneous quantification of various flavonoids, isoflavonoids, and phytohormones extracted from Medicago truncatula leaves 一种快速LC-MS/MS方法用于同时定量从截茎苜蓿叶中提取的各种黄酮类化合物、类黄酮和植物激素

IF 1.3 4区化学

Journal of Liquid Chromatography & Related Technologies Pub Date : 2021-10-02 DOI: 10.1080/10826076.2022.2040028

N. Bisht, Arunima Gupta, Pallavi Awasthi, Atul Goel, D. Chandran, Neha Sharma, Nirpendra Singh

{"title":"Development of a rapid LC-MS/MS method for the simultaneous quantification of various flavonoids, isoflavonoids, and phytohormones extracted from Medicago truncatula leaves","authors":"N. Bisht, Arunima Gupta, Pallavi Awasthi, Atul Goel, D. Chandran, Neha Sharma, Nirpendra Singh","doi":"10.1080/10826076.2022.2040028","DOIUrl":"https://doi.org/10.1080/10826076.2022.2040028","url":null,"abstract":"Abstract Flavonoids, isoflavonoids, and phytohormones are small metabolites of plants, which are involved in the regulation of plant development and/or defense against pathogens. Quantitation of these metabolites in plants is important to basic research on crop protection and in the food and herbal industry. Hence, a robust, reliable, and sensitive method is required for the analysis of these compounds in plant samples. In the present study, a high-performance liquid chromatographic-tandem mass spectrometric (HPLC–ESI-MS/MS) method was developed and validated for the determination of isoflavonoids including genistein, daidzein, formononetin, biochanin A, 2′-hydroxyformononetin, 2′-methoxyformononetin, medicarpin, flavonoids; liquiritigenin, naringenin, and phytohormones; salicylic acid and jasmonic acid in Medicago truncatula. The analytes were separated by means of C-18, reversed-phase chromatography, and detected using QTRAP mass spectrometer. The on-column limit of detection of all the analytes was as low as 0.03 pg, whereas the limit of quantification of all the compounds was observed up to 0.1 pg levels. Further the method was also validated in terms of selectivity, linearity (r 2 > 0.99), recovery (90.6–110.3%), accuracy (RE% ≤ 3%) and precision (RSD% ≤ 3%). As a proof of concept, the developed method was successfully used for the quantitation of these metabolites from leaf extract of M. truncatula. Graphical Abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2021-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48953714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

IF 1.3 4区化学

Journal of Liquid Chromatography & Related Technologies Pub Date : 2021-10-02 DOI: 10.1080/10826076.2022.2072329

A. Bana, P. Mehta

{"title":"Similarity assessment of charge variants for bevacizumab biosimilar formulations using imaged capillary isoelectric focusing","authors":"A. Bana, P. Mehta","doi":"10.1080/10826076.2022.2072329","DOIUrl":"https://doi.org/10.1080/10826076.2022.2072329","url":null,"abstract":"Abstract The imaged capillary isoelectric focusing method was developed and validated for charge variant analysis of the bevacizumab innovator product and its biosimilar formulations (N = 2). The optimized method consists of; 4 M urea, 2.0 mM iminodiacetic acid, 0.35% m/v methylcellulose, 5.0 mM L-arginine, 4.0% v/v carrier ampholytes (narrow-range isoelectric point 5–8 and 8–10.5 with ratio of 1:3), 60 mM sodium hydroxide and 30 mM phosphoric acid as catholyte and anolyte solutions. The bevacizumab innovator and biosimilars formulations were observed with two acidic peaks followed by the main peak and two basic peaks. The validation of the developed method was done by performing intraday (n = 6) and interday (n = 9) precision studies with observed RSD < 2.0%. The limit of detection and limit of quantification of the developed method was found to be 0.016 and 0.048 mg mL−1. The charge variant profiles observed for innovator and biosimilar formulations (N = 2) were then applied for statistical comparison using one-way ANOVA, Dunnett’s test. This study can serve as a reference to biosimilar manufacturers and startups on how to assess the charge variant profiles of bevacizumab during the manufacturing process. Graphical Abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2021-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48491383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2