Separation optimization and quantitative analysis of phytoestrogens employing reverse-phase high-performance liquid chromatography with UV-VIS detection

IF 1 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of Liquid Chromatography & Related Technologies Pub Date : 2021-12-14 DOI:10.1080/10826076.2022.2110118

Michael Payette, Marta R. M. Lima, W. M. Coleman, M. Ashraf-Khorassani

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引用次数: 1

Abstract

Abstract The effect of different acidic modifiers on the separation of seven selected phytoestrogens by reverse-phase high-performance liquid chromatography has been described. Variation in the hydrocarbon chain length of organic acids as represented by acetic acid, propanoic acid, iso-butyric acid, and n-butyric acid as additives to the mobile phase had minimal effect on the separation of phytoestrogens but notably reduced sample turnaround times relative to the turnaround time linked with formic acid. Two different C18 columns were evaluated in attempts of optimize the phytoestrogen separations. Formic acid as a modifier in the mobile phase and an Atlantis C18 column provided the best overall separation at optimum gradient conditions. A higher acid concentration in the mobile phase did not improve the phytoestrogen separation. Higher column temperature (40 °C) provided an improved separation. Finally, the optimized method was used to quantify different phytoestrogens in selected different botanical samples. For this purpose, both internal and external standard methods were compared to obtain the quantitative results. Graphical Abstract

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紫外可见反相高效液相色谱法优化植物雌激素分离及定量分析

本文介绍了不同酸性改性剂对反相高效液相色谱法分离7种植物雌激素的影响。以乙酸、丙酸、异丁酸和正丁酸为流动相添加剂的有机酸的烃链长度的变化对植物雌激素的分离影响最小，但相对于与甲酸相关的周转时间，样品周转时间显著缩短。为了优化植物雌激素的分离，对两种不同的C18柱进行了评价。在流动相中作为改性剂的甲酸和Atlantis C18柱在最佳梯度条件下提供了最佳的整体分离。流动相中较高的酸浓度并不能改善植物雌激素的分离。较高的柱温度（40 °C）提供了改进的分离。最后，使用优化的方法对所选不同植物样品中的不同植物雌激素进行了定量。为此，对内部和外部标准方法进行了比较，以获得定量结果。图形摘要

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来源期刊

Journal of Liquid Chromatography & Related Technologies 化学-分析化学

CiteScore

2.80

自引率

0.00%

发文量

审稿时长

4.9 months

期刊介绍： The Journal of Liquid Chromatography & Related Technologies is an internationally acclaimed forum for fast publication of critical, peer reviewed manuscripts dealing with analytical, preparative and process scale liquid chromatography and all of its related technologies, including TLC, capillary electrophoresis, capillary electrochromatography, supercritical fluid chromatography and extraction, field-flow technologies, affinity, and much more. New separation methodologies are added when they are developed. Papers dealing with research and development results, as well as critical reviews of important technologies, are published in the Journal.