Journal of Liquid Chromatography & Related Technologies最新文献

{"title":"Applying machine learning on high-performance thin-layer chromatography using the complementary developing solvents concept","authors":"Thi Kieu Tiên Do, Ilona Trettin, Mona Hänni, Eike Reich","doi":"10.1080/10826076.2023.2284707","DOIUrl":"https://doi.org/10.1080/10826076.2023.2284707","url":null,"abstract":"Predicting chromatographic results is a difficult task for many analysts, especially in Thin-Layer Chromatography (TLC) where reproducibility is always a critical point. The availability of suitabl...","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":"14 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138540054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A modern chromatographic energy-saving approach for the analysis of doxycycline, ambroxol, and its precursor and impurity: Green metric assessment and comparison","authors":"Selvia M. Adly, Mohamed A. Elsayed, Safa’a M. Riad, Hany H. Monir","doi":"10.1080/10826076.2023.2284729","DOIUrl":"https://doi.org/10.1080/10826076.2023.2284729","url":null,"abstract":"The ternary mixture of doxycycline hyclate (DOX), ambroxol hydrochloride (AMB), and ambroxol synthetic precursor and process impurity (IMP) as chemically identified in the British Pharmacopeia as 2...","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":"40 2 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138540068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect-directed profiling of bee pollen versus propolis","authors":"Gertrud E. Morlock, Fang Yang","doi":"10.1080/10826076.2023.2284708","DOIUrl":"https://doi.org/10.1080/10826076.2023.2284708","url":null,"abstract":"Natural bee products are increasingly used in cosmetics, health products, and food supplements. Bee pollen and propolis are quite different products, although the processing by the bees is for the ...","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":"30 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138543560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of TLC-smartphone method for the analysis of carbamazepine in plasma","authors":"Mohamed Ali Soussi, Youssef Mallek, Wiem Ben Ayed, Slah eddine Liouane, Wahiba Douki","doi":"10.1080/10826076.2023.2284722","DOIUrl":"https://doi.org/10.1080/10826076.2023.2284722","url":null,"abstract":"A new, simple, rapid, low-cost, and robust thin layer chromatography-smartphone method has been developed for the qualitative and quantitative determination of carbamazepine in human plasma. Liquid...","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":"36 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138540071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel UHPLC-MS/MS method for trace level identification and quantification of genotoxic impurity 2-(2-chloroethoxy) ethanol in quetiapine fumarate","authors":"Mahammad Ali Shaik, Krishna Moorthy Manchuri, Devanna Nayakanti","doi":"10.1080/10826076.2023.2276283","DOIUrl":"https://doi.org/10.1080/10826076.2023.2276283","url":null,"abstract":"AbstractA selective and sensitive new reversed-phase method was developed and validated by using Waters Acquity UHPLC system coupled with Waters Micromass Quattro PremierXE (MS/MS) to identify and quantify the genotoxic impurity 2-(2-chloroethoxy) ethanol at trace level (∼2.0 ppm) in quetiapine fumarate. The method used positive ion electrospray ionization with a multiple reaction monitoring detection mode using ACE3 C18(100 mm × 4.6 mm × 3.0 µm) column. For analysis, an isocratic program was developed using 0.01 M ammonium acetate in Milli-Q water (mobile phase A) and methanol (mobile phase B) in 20:80v/v ratio. Elution of 2-(2-chloroethoxy) ethanol was monitored using triple quadrupole mass spectrometer with an injection volume of 10 μl, a column oven temperature of 40 °C, an auto-sampler temperature of 15 °C, and flow rate of 0.5 mL/min. The retention time of 2-(2-chloroethoxy) ethanol was observed at 2.38 min. The LOQ and LOD were determined at concentrations of 0.235 ppm and 0.070 ppm respectively. The correlation coefficient R2 was 0.9983 and the percent recoveries of method range from 98.1% to 114.0%. The method was successfully validated according to International Council for Harmonization requirements (ICH Q2(R1) and ICH M7(R1)) for genotoxic impurities. The method was sensitive, selective, accurate and precise and therefore can be used for identification and quantification of genotoxic impurity 2-(2-chloroethoxy) ethanol in quetiapine fumarate.Keywords: 2-(2-chloroethoxy) ethanolgenotoxic impurityquetiapineUHPLC-MS/MSMultiple reaction monitoring (MRM) Disclosure statementNo potential conflict of interest was reported by the author(s).AcknowledgementsThe authors thank the management of Kshetra Analytics Ltd (Hyderabad, TS, India) and Jawaharlal Nehru Technological University, Anantapur (AP, India) for their support and encouragement to conduct this research.The author’s own views were expressed in this paper. The authors Shaik Mahammad Ali and Manchuri Krishna Moorthy are currently working for Novartis Health Care Pvt Ltd, Hyderabad, India.","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":" 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135290780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rapid and eco-friendly method for determination of dictamnine, obacunone, and fraxinellone in Cortex Dictamni by LC–MS and matrix solid-phase dispersion extraction","authors":"Zhengming Qian, Qinggui Lei, Chuanxi Wang, Qi Huang, Wenqing Li, Wenhao Wang","doi":"10.1080/10826076.2023.2274848","DOIUrl":"https://doi.org/10.1080/10826076.2023.2274848","url":null,"abstract":"","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135871458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-friendly design of experiment based approach for estimation of azelnidipine and olmesartan medoxomil in RP-HPLC: Greenness appraisal","authors":"Naveenarani Dharuman, Lakshmi Karunanidhi Santhana, Manikandan Krishnan","doi":"10.1080/10826076.2023.2270734","DOIUrl":"https://doi.org/10.1080/10826076.2023.2270734","url":null,"abstract":"AbstractThe analytical chemistry community has prioritized the implementation of green analytical methods in recent years to minimize the adverse effect on the environment and human beings. The combination of Azelnidipine (AZD) and Olmesartan medoxomil (OLM) was used to manage hypertension. Thus, the present study aimed to develop an RP-HPLC technique to determine AZD and OLM simultaneously by the combined framework of design of experiments and green analytical principles. A central composite design (CCD) was employed for method optimization, with ethanol, and flow rate was selected as critical variables. The effective separation was performed by Agilent Eclipse Plus (C18, 250 × 4.6 mm i.d, 5 μm) with a mobile phase comprising ethanol and 1% v/v aqueous acetic acid in the ratio of 49.5:50.5 I flowing at 1 mL/min, the detection wavelength at 250 nm. The retention time for AZD and OLM were 6.362 and 3.323, respectively. Linearity was perceived over the concentration ranges of 6.4–9.6 and 16–24 μg/mL for AZD and OLM, respectively. The green character of the proposed method showed excellent green results. This innovative eco-friendly, design of experiment technology was adequate for regular analysis of AZD and OLM in pharmaceutical dosage form without harming the environment.Keywords: Azelnidipinecentral composite designgreen HPLColmesartan medoxomil AcknowledgementThe authors are grateful to SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, for motivating us to carry out this research.Disclosure statementNo potential conflict of interest was reported by the author(s)","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":"45 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136381260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient and reliable CE-SDS separations by the high-speed turbo CE-SDS ^® cartridge","authors":"Robert Minkner, Udo Burger, Louis Malz, Susanne Doerks, Hermann Wätzig","doi":"10.1080/10826076.2023.2269233","DOIUrl":"https://doi.org/10.1080/10826076.2023.2269233","url":null,"abstract":"AbstractCapillary electrophoresis-sodium dodecyl sulfate (CE-SDS) is a modern form of SDS-PAGE that uses capillary electrophoresis to separate proteins by size. The technique is based on the sieving effect of net-like structures in the capillary. In this study, we compared the Maurice Turbo CE-SDS Cartridge™ with the established Maurice CE-SDS PLUS Cartridge, both developed for the Maurice CE system. The Turbo cartridge was found to be up to 4–5 times faster than the PLUS cartridge, while maintaining similar separation efficiency. The limit of quantitation (LOQ) for the Turbo cartridge was estimated to be 0.1 mg/mL with a signal-to-noise ratio (S/N) of 18.55. The linearity of the cartridge was verified in the range of 0.1–4 mg/mL, with correlation coefficient (R2) values of at least 0.99. The separation efficiency remained constant over long series of measurements, and the relative migration time of proteins in a mixture remained stable over 1,000 injections. These results demonstrate that the Turbo CE-SDS Cartridge is a very useful tool for fast and efficient analysis of protein (mixtures). The shorter analysis time can save significant resources by reducing the time and consumables required for analysis, while the high LOQ and linearity ensure that the method is sensitive and accurate.Keywords: CECE-SDScomparisongel electrophoresisvalidation Disclosure StatementThe authors declare there is no Complete of Interest at this study.AcknowledgmentsNot applicableAuthors’ contributionsRM performed the experiments, wrote the manuscript, and designed the study. UB participated in study design and partly supervised the project. LM carried out repetitive experiments as instructed. SD partly supervised the project. HW supervised the entire project and revised the manuscript. All authors read and approved the final manuscript.Availability of data and materialsAll relevant data generated or analyzed during this study are included in this published article and its additional file. The missing datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request.Competing interestsUdo Burger and Susanne Doerks are employes of ProteinSimple (a Bio-Techne Brand).Consent for publicationNot applicable.Declaration of interestUdo Burger and Susanne Doerks are employees of Bio-Techne.Ethics approval and consent to participateNot applicable.Additional informationFundingThis work was supported by Protein Simple (a Brand of Bio-Techne) with materials.","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135888610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-performance liquid chromatography with fluorescence detection method for measuring colistin in human serum and its application in critically ill patients treated with colistin methanesulfonate sodium","authors":"Mohd Shafie Zabidi, Ruzilawati Abu Bakar, Nurfadhlina Musa, Suzana Mustafa, Wan Nasrudin Wan Ismail, Siti Nur Aziela Ab Manap, Nur Aishah Che Mohd Zaid, Wan Nazirah Wan Yusuf","doi":"10.1080/10826076.2023.2262002","DOIUrl":"https://doi.org/10.1080/10826076.2023.2262002","url":null,"abstract":"AbstractColistin, in the form of colistin methanesulfonate sodium (CMS), has a narrow therapeutic window, and colistin levels must be monitored during treatment. The aim of this study was to develop and validate a high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method for measuring colistin in human serum. Colistin was extracted from human serum using trichloroacetic acid and methanol for protein precipitation, followed by in-solid phase extraction derivatization with 9-fluorenylmethyl chloroformate. HPLC-FLD system using a reverse-phase HPLC C18 column and a mobile phase consisting of acetonitrile, tetrahydrofuran, and water (80%:4%:16%) were used for the quantification of colistin A, colistin B, and polymyxin B (internal standard) in human serum. The extraction recoveries were between 71% and 103%. Linear calibration curves were obtained for colistin in concentrations from 0.3 to 8.0 μg/mL, with good fit (r2 = 0.9993). The lower limit of quantitation was 0.3 μg/mL. The intra- and inter-day precision of the assay was 0.5–5% and 3.5–9.4%, respectively. The accuracy ranged from 98% to 100%. This validated method was successfully applied to the analysis of serum-receiving CMS in critically ill patients. This method will be useful to assist colistin dosage adjustment based on individual blood colistin levels for optimization of colistin therapy.Keywords: Colistin methanesulfonate sodiumcolistincritically ill patientsHPLC-FLDTDM AcknowledgementThe authors would like to thank the Director General of Health Malaysia for his permission to publish this article. We would also like to acknowledge the recruited patients and family, nurses, and staff members of the intensive care unit of Hospital Raja Perempuan Zainab II, Kota Bahru for their cooperation in conducting the research. The authors would also like to acknowledge staff members of Laboratory Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia for their support and help.Disclosure statementNo potential conflict of interest was reported by the author(s).Additional informationFundingThis study was supported by USM Bridging Grant No: 304.PPSP.6316240 and GIPS-PhD Grant No: 311/PPSP/4404810. The first author was supported by Malaysia Ministry of Health, Hadiah Latihan Persekutuan (HLP). Institute of Postgraduate Studies, Universiti Sains Malaysia","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135246229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expressional Study of Permeability Glycoprotein and Multidrug Resistance Protein 1 in Drug-resistant Mesial Temporal Lobe Epilepsy.","authors":"Mandeep Kaur, Tulika Gupta, Mili Gupta, Navneet Singla, Parampreet S Kharbanda, Yogender Singh Bansal, Daisy Sahni, Bishan Das Radotra, Sunil Kumar Gupta","doi":"10.32598/bcn.2021.2554.3","DOIUrl":"10.32598/bcn.2021.2554.3","url":null,"abstract":"<p><strong>Introduction: </strong>About 30% of patients with epilepsy do not respond to anti-epileptic drugs, leading to refractory seizures. The pathogenesis of drug-resistance in mesial temporal lobe epilepsy (MTLE) is not completely understood. Increased activity of drug-efflux transporters might be involved, resulting in subclinical concentrations of the drug at the target site. The major drug-efflux transporters are permeability glycoprotein (<i>P-gp</i>) and multidrug-resistance associated protein-1 (<i>MRP-1</i>). The major drawback so far is the expressional analysis of transporters in equal numbers of drug-resistant epileptic tissue and age-matched non-epileptic tissue.</p><p><strong>Methods: </strong>We have studied <i>P-gp</i> and <i>MRP-1</i> drug-efflux transporters in the sclerotic hippocampal tissues resected from the epilepsy surgery patients (n=15) and compared their expression profile with the tissues resected from non-epileptic autopsy cases (n=15).</p><p><strong>Results: </strong>Statistically significant over expression of both <i>P-gp</i> (P<0.0001) and <i>MRP-1</i> (P=0.01) at gene and protein levels were found in the MTLE cases. The fold change of <i>P-gp</i> was more pronounced than <i>MRP-1</i>. Immunohistochemistry of the patient group showed increased immunoreactivity of <i>P-gp</i> at blood-brain barrier and increased reactivity of <i>MRP-1</i> in the parenchyma. The results were confirmed by confocal immunofluorescence microscopy.</p><p><strong>Conclusion: </strong>Our results suggested that <i>P-gp</i> in association with <i>MRP-1</i> might be responsible for the multi-drug resistance in epilepsy. <i>P-gp</i> and <i>MRP-1</i> could be important determinants of bio availability and tissue distribution of anti-epileptic drugs in the brain which can pharmacologically inhibited to achieve optimal drug penetration to target site.</p>","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":"30 1","pages":"615-630"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11016880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81673958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}