Efficient and reliable CE-SDS separations by the high-speed turbo CE-SDS ® cartridge

Abstract

AbstractCapillary electrophoresis-sodium dodecyl sulfate (CE-SDS) is a modern form of SDS-PAGE that uses capillary electrophoresis to separate proteins by size. The technique is based on the sieving effect of net-like structures in the capillary. In this study, we compared the Maurice Turbo CE-SDS Cartridge™ with the established Maurice CE-SDS PLUS Cartridge, both developed for the Maurice CE system. The Turbo cartridge was found to be up to 4–5 times faster than the PLUS cartridge, while maintaining similar separation efficiency. The limit of quantitation (LOQ) for the Turbo cartridge was estimated to be 0.1 mg/mL with a signal-to-noise ratio (S/N) of 18.55. The linearity of the cartridge was verified in the range of 0.1–4 mg/mL, with correlation coefficient (R2) values of at least 0.99. The separation efficiency remained constant over long series of measurements, and the relative migration time of proteins in a mixture remained stable over 1,000 injections. These results demonstrate that the Turbo CE-SDS Cartridge is a very useful tool for fast and efficient analysis of protein (mixtures). The shorter analysis time can save significant resources by reducing the time and consumables required for analysis, while the high LOQ and linearity ensure that the method is sensitive and accurate.Keywords: CECE-SDScomparisongel electrophoresisvalidation Disclosure StatementThe authors declare there is no Complete of Interest at this study.AcknowledgmentsNot applicableAuthors’ contributionsRM performed the experiments, wrote the manuscript, and designed the study. UB participated in study design and partly supervised the project. LM carried out repetitive experiments as instructed. SD partly supervised the project. HW supervised the entire project and revised the manuscript. All authors read and approved the final manuscript.Availability of data and materialsAll relevant data generated or analyzed during this study are included in this published article and its additional file. The missing datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request.Competing interestsUdo Burger and Susanne Doerks are employes of ProteinSimple (a Bio-Techne Brand).Consent for publicationNot applicable.Declaration of interestUdo Burger and Susanne Doerks are employees of Bio-Techne.Ethics approval and consent to participateNot applicable.Additional informationFundingThis work was supported by Protein Simple (a Brand of Bio-Techne) with materials.