Journal of lasers in medical sciences最新文献

{"title":"Selective Response of Cancer and Normal Cells to 808 nm near Infrared Low Level Laser Irradiation: An <i>in Vitro</i> Study.","authors":"Sarah Shakir Al-Mafarjy, Nursakinah Suardi, Hamed Alnagran, Naser Mahmoud Ahmed, Daruliza Kernain Mohd Azman, Huda Hisham Alkatib","doi":"10.34172/jlms.50168","DOIUrl":"10.34172/jlms.50168","url":null,"abstract":"<p><p><b>Introduction:</b> This study aims to investigate laser irradiation therapy as a potential non-invasive treatment for breast cancer, introducing a promising alternative with the potential for the selective inhibition of cancerous cells while minimizing damage to surrounding healthy tissues. <b>Methods:</b> In this study, the utilization of cell culture stands out as an excellent methodology for evaluating both the effects and dosage of a given treatment. This study was conducted to evaluate the selective effect of low-level near-infrared (NIR) laser at an 808 nm wavelength, along with single and double irradiation with 5-minute intervals, on human breast cancer (MDA-MB-231) cells and human foreskin fibroblasts (Hs-27) cells using different laser powers and exposure times. The experiment involved exposing both cell lines to an NIR laser at 25, 43, 120, and 300 mW for 1, 5, 10, and 15 minutes. Cell viability was assessed via the MTT assay. <b>Results:</b> The outcomes revealed disparate responses in both cell types used in this study. The double irradiation procedure with a 15-minute exposure at 25, 43, 120, and 300 mW displayed the most inhibition of cancer cell growth among the applied durations. Notably, using 300 mW during 15-minute double irradiation resulted in the highest cancer cell growth inhibition at 4.67%. Conversely, normal cells showed enhanced proliferation across most powers and exposure times applied. However, a considerable reduction in the cell viability of normal cells was evident following 15-minute double irradiation at 300 mW. This indicates that normal cells have been negatively affected by this power. Caution is advised. <b>Conclusion:</b> The results suggest that NIR laser therapy at 808 nm with different output powers and exposure times significantly inhibits MDA-MB-231 cells, and this approach exhibits promising potential for inducing cancer cell death.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"17 ","pages":"e50168"},"PeriodicalIF":1.7,"publicationDate":"2026-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13527706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148865216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ablation Depth and Surface Morphology of Dentin After Irradiation with a Tunable TEA-CO₂ Laser in the Wavelength Range of 9.3 - 10.6 µm.","authors":"Hadiseh Ghalkhanbaz, Maryam Ilchi Ghazaani, Batool Sajad","doi":"10.34172/jlms.2026.09","DOIUrl":"10.34172/jlms.2026.09","url":null,"abstract":"<p><p><b>Introduction:</b> This study evaluated morphological and structural changes in extracted human dentin following irradiation with a pulsed tunable TEA-CO₂ laser (9-11 μm) using scanning electron microscopy (SEM). The ablation rate was quantitatively determined at different wavelengths and pulse numbers while assessing preservation of dentinal tubules. <b>Methods:</b> Nearly flat dentin specimens were irradiated at four wavelengths (9.3, 9.6, 10.3, and 10.6 μm) with a constant energy density of 7.36 J/cm², pulse width of 200 ns, and repetition rate of 1 Hz. Samples were grouped by cooling method (air or water) and exposed to 1, 5, 10, 20, or 30 pulses. Surface morphology was examined by optical microscopy and SEM. Single-pulse ablation depth and ablation rate (μm/pulse) were measured using a profilometer under sub-threshold thermal damage conditions. Dentinal tubule occlusion after single-shot irradiation was compared with untreated dentin controls. <b>Results:</b> Air-cooled samples exhibited thermal damage, including carbonization and cracking. SEM analysis showed that water cooling led to greater thermal damage at high pulse numbers, particularly at wavelengths near 9 μm, corresponding to strong hydroxyapatite absorption and a lower ablation threshold. Up to 10 pulses, no cracks or peripheral thermal damage were observed at any wavelength. For single-pulse irradiation, the greatest ablation depth occurred at 10.3 μm with complete tubule occlusion, followed by 9.6 μm. At five pulses, maximum ablation depth was again observed at 10.3 μm (complete occlusion) and 9.3 μm (partial occlusion). <b>Conclusion:</b> These findings indicate that appropriate selection of wavelength, pulse number, and cooling method enables controlled dentin removal using a pulsed TEA-CO₂ laser without peripheral tissue damage. Wavelength-dependent absorption and cooling conditions are critical for optimizing ablation efficiency while preserving dentinal tubule integrity.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"17 ","pages":"e9"},"PeriodicalIF":1.7,"publicationDate":"2026-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13527704/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148864724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Photodynamic Antimicrobial Therapy Using 405 nm Laser Diode and Moringa Oleifera-Derived Silver Nanoparticles against Pathogenic Bacteria.","authors":"Suryani Dyah Astuti, Agustina Dwi Cahyani, Endarko, Sri Dewi Astuty, Ahmad Khalil Yaqubi, Muhammad Nurdin, Dezy Zahrotul Istiqomah Nurdin, Ardiansyah Syahrom","doi":"10.34172/jlms.2026.08","DOIUrl":"10.34172/jlms.2026.08","url":null,"abstract":"<p><p><b>Introduction:</b> This study examines the antibacterial capabilities of Moringa oleifera green-synthesized silver nanoparticles (AgNPs-MO), in combination with UV light and a blue laser set at 405 nm, against <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. Higher concentrations of AgNPs-MO enhanced the antimicrobial activity when exposed to both light treatments. The findings suggest that Moringa oleifera-derived photosensitizers improve the efficacy of photodynamic antimicrobial therapy, offering an eco-friendly approach for enhanced infection control. <b>Methods:</b> The experimental design comprised four groups: three bacterial groups (A1/A2 and A3/A4) exposed to laser irradiation at varying concentrations and durations of silver nanoparticles, and a control group (T0) that was not exposed to laser irradiation. A photosensitizer produced from Moringa oleifera was also administered to bacteria in groups A2 and A4, and different laser exposure durations were then applied. After incubation, bacterial colonies were measured with a Quebec colony counter. <b>Results:</b> Statistical analysis using a two-way ANOVA factorial test and post-hoc Tukey test revealed significant reductions in bacterial viability. <i>E. coli</i> exhibited viability reductions of 70.90%, 76.44%, and 79.87% with mmol L-1, mmol L-1.5, and mmol L-2 AgNPs-MO after 180 seconds of laser exposure, respectively. Similarly, <i>S. aureus</i> showed viability reductions of 97.48%, 95.94%, and 94.72% under the same conditions. <b>Conclusion:</b> At an energy density of 3.44 J/cm², our approach demonstrated notable efficacy, achieving a 79.87%±1.92% inactivation of <i>E. coli</i> and a 97.48%±0.78% inactivation of <i>S. aureus</i>. This study suggests promising applications for the combined use of a blue laser, UV radiation, silver nanoparticles, and Moringa oleifera extract in combating infectious bacteria.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"17 ","pages":"e8"},"PeriodicalIF":1.7,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13527705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148865312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Anticancer Effect of Rhodium Nanoparticle-based Photodynamic Therapy via Protein-Protein Interaction Network Analysis.","authors":"Nikoo Hossein-Khannazer, Babak Arjmand, Zahra Razzaghi, Farideh Razi, Fatemeh Bandarian, Alireza Ahmadzadeh","doi":"10.34172/jlms.2026.07","DOIUrl":"10.34172/jlms.2026.07","url":null,"abstract":"<p><p><b>Introduction:</b> Photodynamic therapy, as an efficient and safe method, has attracted the attention of experts. This therapeutic method is based on the application of photosensitizers and light radiation. This study was designed to assess the possible molecular mechanism of rhodium nanoparticle-based photodynamic therapy through protein-protein interaction (PPI) network analysis of proteomic data from the literature. <b>Methods:</b> Proteomic data about rhodium nanoparticle-based photodynamic therapy effect on the HeLa cell line proteome were retrieved from the literature and were included in the CluePedia application of Cytoscape software to create a directed PPI network. The network was analyzed, and the crucial targeted proteins were identified and compared with genes in GeneCards for \"HeLa cell line\" and \"cervical cancer\". The common gene and protein were selected and discussed. <b>Results:</b> A directed PPI network of 105 proteins was formed. Six sub-networks were selected for further investigation. Comparison of the PPI data and the genes from the GeneCards database led to the introduction of HLA-B, CYCS, CD44, HSPB1, and RBBP4 as the critical targeted proteins by the applied treatment. <b>Conclusion:</b> In conclusion, a sub-network including HLA-B, CYCS, CD44, and HSPB1 and another sub-network containing RBBP4 and its neighbors were highlighted as the core of molecular effects of the applied rhodium nanoparticle-based photodynamic therapy.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"17 ","pages":"e7"},"PeriodicalIF":1.7,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13224329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148150085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Overview of How HNCs Radiotherapy Affects Oral Microbiota: Shifts, Complications, and Management Strategies.","authors":"Saman Ayoubi, Mohammad Rahmanian, Neda Hakimiha, Mostafa Rezaei Tavirani, Somayeh Jahani-Sherafat","doi":"10.34172/jlms.2026.06","DOIUrl":"10.34172/jlms.2026.06","url":null,"abstract":"<p><p><b>Introduction:</b> Radiotherapy (RT) is a primary treatment for head and neck cancers (HNCs). However, it can lead to acute and chronic side effects that diminish patients' quality of life. RT-induced radiation can damage tissues and alter the oral microbiota. Multiple studies indicate that the oral microbiota significantly influences the development of these complications. <b>Methods:</b> This research aims to examine how radiotherapy affects the oral microbiota thoroughly, explore the link between these changes and clinical issues, and evaluate strategies for prevention and management. <b>Results:</b> RT damages salivary glands and the oral mucosa, leading to xerostomia (dry mouth) and oral mucositis. These physical and chemical changes alter the oral environment, resulting in dysbiosis. This dysbiosis is characterized by reduced microbial diversity and an increase in pathogenic species, including cariogenic bacteria like Streptococcus mutans and Lactobacillus, as well as inflammatory bacteria such as Prevotella, Fusobacterium, and Candida albicans. The review suggests that this dysbiotic profile may significantly contribute to the worsening of oral mucositis (through inflammatory and LPS-dependent pathways), promote radiation-induced caries (due to increased xerostomia and acidogenic bacteria), elevate the risk of oropharyngeal candidiasis, and play a role in osteoradionecrosis development. <b>Conclusion:</b> The findings indicate that the oral microbiota is not just a secondary factor in RT-induced damage but plays an active role in worsening RT-related side effects. Gaining a precise understanding of the oral microbiota as a potential therapeutic target is crucial, and incorporating oral microbiology into oncology protocols can greatly enhance patients' quality of life and pave the way for new approaches in cancer supportive care.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"17 ","pages":"e6"},"PeriodicalIF":1.7,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13224326/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148150130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photothermal Therapy Using Ag/TiO2 Core-Shell Nanoparticles Against Breast Cancer Cell Line: an <i>In Vitro</i> and <i>In Vivo</i> Study.","authors":"Maeda H Mohammad, Omar Hussein Khalaf, Huda Mahmood Al-Attar, Aous Kahtan Almzaien, Ali Hussain Alwan, Adnan Khazaal Ajeel, Haydar Adnan Hassoon, Rasha Abd Al-Amir Hussein, Aysar A Ahmed","doi":"10.34172/jlms.2026.05","DOIUrl":"10.34172/jlms.2026.05","url":null,"abstract":"<p><p><b>Introduction:</b> Pulsed Laser Ablation in Liquid (PLAL) is considered a facile and green method for synthesizing nanoparticles (NPs) with no byproducts formed during the synthesis process. Compared to other traditional treatment strategies, photothermal therapy (PTT) is considered a powerful alternative anti-tumor treatment methods. Ag/TiO₂ core-shell NPs were determined as a photothermal agent in the NIR-II window in the biomedical field. The anti-tumor effect (<i>in vitro</i>) and safety study (<i>in vivo</i>) of Ag/TiO₂ core-shell NPs were conducted in the current study. <b>Methods:</b> TiO₂ nanoparticle colloid was prepared by PLAL using a fiber laser (1060 nm), In order to achieve a nanoparticle core-shell, Ag NPs were added to the as-prepared nanoparticle colloid. The anti-tumor effect of the produced Ag/TiO₂ core-shell NPs, in D.W. and PBS as solvents, was investigated against the AMJ13 cell line both with and without using the laser at 1060 nm as the ablation source for the PTT <i>in vitro</i>. Also, histopathological changes following a single injection of the Ag/TiO₂ in mice were evaluated. <b>Results:</b> <i>In vitro</i> results showed that the Ag/TiO₂ in PBS had anti-breast cancer cell line compared with REF normal cell line, while Ag/TiO₂ that was prepared in D.W. was biocompatible. Therefore, the anti-tumor potential impact of Ag/TiO₂ on PBS was selected to be tested as a photothermal agent <i>in vitro</i>. Interestingly, the results of the <i>in vivo</i> study showed Ag/TiO₂ no significant histopathological changes on mouse tissue. Moreover, the PTT study demonstrated that the best working laser parameters were 5W within 5 minutes, which achieved more than 57% inhibition percentage of tumor cell growth compared to the non-irradiated cancer cells. <b>Conclusion:</b> Ag/TiO₂ core-shell nanoparticle is concluded to be a promising PTT agent in the NIR-II window against the AMJ13 breast cancer cell line, and it was safe on normal tissue <i>in vivo</i>.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"17 ","pages":"e5"},"PeriodicalIF":1.7,"publicationDate":"2026-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13224323/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148150160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Synergic Effects of Molybdenum Disulfide Treatment and Near-Infrared Radiation on Human Mesenchymal Stem Cells.","authors":"Alireza Omidi Farzin, Babak Arjmand, Nastaran Asri, Zahra Razzaghi, Farideh Razi, Fatemeh Bandarian, Reza M Robati, Mitra Rezaei, Reza Vafaee, Parnia Rahnamay Farnood","doi":"10.34172/jlms.2026.04","DOIUrl":"10.34172/jlms.2026.04","url":null,"abstract":"<p><p><b>Introduction:</b> Molybdenum disulfide (MoS2), as an anticancer reagent, can absorb near-infrared (NIR) wavelengths in phototherapy. In the present study, the synergic effect of MoS2 (in nanostructure) and NIR radiation on human mesenchymal stem cells (hMSCs) was evaluated via protein-protein interaction (PPI) network analysis. <b>Methods:</b> Gene expression profiles of hMSCs were extracted from the Gene Expression Omnibus (GEO) database and analyzed via PPI network analysis. The validity of findings was assessed via the Kaplan-Meier Plotter. <b>Results:</b> FN1, ACTA2, CCL2, CXCL8, FBN1, and LEP genes were selected as hub genes among 121 recognized differentially expressed genes (DEGs) as the targeted genes by the synergic effects of MoS2 and NIR radiation. Kaplan-Meier Plotter analysis demonstrated that FN1 suppression by MoS2 and NIR radiation can significantly increase the overall survival of patients with gastric cancer, lung cancer, ovarian cancer, and myeloma. <b>Conclusion:</b> In conclusion, findings indicate that the anticancer property of MoS2 is intensified in the condition of NIR radiation. Therefore, MoS2 is a suitable photosensitizer reagent in photodynamic therapy.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"17 ","pages":"e4"},"PeriodicalIF":1.7,"publicationDate":"2026-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13224324/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148150086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Propolis as an Intracanal Agent in Combination with an Nd:YAG Laser for Bacterial Reduction.","authors":"Mahdieh Naghizadeh, Elaheh Fakhri, Hossein Samadi Kafil, Hosein Eslami, Azam Valian","doi":"10.34172/jlms.2026.03","DOIUrl":"10.34172/jlms.2026.03","url":null,"abstract":"<p><p><b>Introduction:</b> This study aimed to assess the antimicrobial efficacy of propolis for an intracanal medicament in combination with an Nd:YAG laser against the biofilm of persistent endodontic pathogens: <i>Enterococcus faecalis</i>, <i>Staphylococcus aureus</i> and <i>Candida albicans</i>. <b>Methods:</b> One hundred and sixty-five extracted human premolars were included. Monospecies of four-week biofilms were cultured in root canals after the teeth were prepared chemo-mechanically. Specimens were treated with calcium hydroxide for 24 hours (G1), propolis for 24 hours (G2), Nd:YAG laser (1064 nm, 1.5 w, 15 Hz, 100 mJ/pulse, 200 µm endodontic fiber tip) (G3), propolis medicament followed by Nd:YAG laser (G4), and normal saline as the control group (G5). Dentin chips were obtained with Gates-Glidden #5, 6 and inoculated onto agar plates for microbial growth. The antimicrobial efficacy of each group was evaluated by quantifying the number of colony-forming units. Data were statistically analyzed using the Kruskal-Wallis and Mann-Whitney U test (<i>P</i> value<0.05). <b>Results:</b> All investigated groups reduced the bacterial count compared to normal saline (<i>P</i> value<0.05). Propolis (0.23±0.58, 0.00±0.00, 1.34±1.12 for <i>E.f</i>, <i>S.a</i>, and <i>C.a</i>., respectively) and Nd:YAG laser (3.19±0.51, 3.21±0.37, 3.29±0.52 for <i>E.f</i>, <i>S.a</i>, and <i>C.a</i>., respectively) were more effective in the reduction of all three pathogens during 24 hours, compared to calcium hydroxide (<i>P</i> value<0.05). The application of propolis followed by the Nd:YAG laser resulted in the complete elimination of <i>E. faecalis</i> and <i>S. aureus</i> and a significant reduction in <i>C. albicans</i> (0.60±0.96) (<i>P</i> value<0.05). <b>Conclusion:</b> Propolis alone or in combination with the Nd:YAG laser at low powers is highly effective against endodontic pathogens in comparison with conventional calcium hydroxide medicament.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"17 ","pages":"e3"},"PeriodicalIF":1.7,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13224327/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148150083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Low-Level Light Therapy (LLLT) on Oxidative Stress in the Cutaneous Healing Process: A Literature Review.","authors":"Paola F Irber, Jaíne Dalmolin, Danielli S Da Silva, Cecília C Pimentel, Eduarda R Kozoroski, Felipe B Schuch, Gustavo O Puntel","doi":"10.34172/jlms.2026.02","DOIUrl":"10.34172/jlms.2026.02","url":null,"abstract":"<p><p><b>Introduction:</b> Photobiomodulation (PBM), initially called low-level light therapy (LLLT), is defined as the application of radiation in the form of light for healing since it can modify the cell's behavior to facilitate repair of the tissue. The objective of this review study was to research the known effects of the LLLT on oxidative stress, as well as mitochondrial activity, in the cutaneous healing process. <b>Methods:</b> We included experimental animal studies that evaluated the effects of the LLLT on the oxidative stress related to the cutaneous healing process. In December 2023, research into the literature was conducted in the following databases: PubMed, Embase, and Web of Science. The methodological quality of the studies was evaluated by the adapted PEDro scale. Additionally, a qualitative and descriptive analysis of the included studies was made. <b>Results:</b> Nine studies were included, and all the articles presented significant modulation of oxidative stress, with reductions in oxidizing markers (superoxide anion) and apoptosis activators (cytochrome c), and mainly, increases in antioxidant enzymatic defense (catalase, superoxide dismutase, and glutathione peroxidase). <b>Conclusion:</b> LLLT represents a technique with promising results in the improvement and acceleration of the cutaneous healing process, as well as in the regulation of biochemical reactions related to oxidative stress of the treated areas. Despite that, more studies focusing on the central role of mitochondria in explaining the therapeutic effects of LLLT are necessary.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"17 ","pages":"e2"},"PeriodicalIF":1.7,"publicationDate":"2026-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13224328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148150116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Concise Review of the Impact of Photobiomodulation (PBM) on Neuronal Differentiation in Adipose-Derived Mesenchymal Stem Cells.","authors":"Negar Mobini, Afshan Shirkavand, Shirin Farivar","doi":"10.34172/jlms.2026.01","DOIUrl":"10.34172/jlms.2026.01","url":null,"abstract":"<p><p><b>Introduction:</b> Photobiomodulation (PBM), utilizing low-level light, has shown significant potential in differentiating adipose-derived mesenchymal stem cells (ADMSCs) into neuronal cells. Critical studies have shown that specific laser parameters, such as wavelength and fluence, significantly impact the trans-differentiation outcomes of immortalized ADMSCs. This review aimed to consider this phenomenon. <b>Methods:</b> This review was conducted based on several English articles from Google Scholar, Science Direct, and Medline, such as the National Library of Medicine (NLM) and PubMed from 2010 to 2025. The term 'ADMSCs' was searched combined with the following keywords: \"Photobiomodulation\" OR \"low-level laser irradiation\" OR \"low-power\" laser OR \"low-intensity laser\" OR \"Photobiostimulation\". <b>Results:</b> This investigation demonstrated that PBM is a non-invasive, light-based method for facilitating the neuronal differentiation of the ADMSCs. It is effective for increased production of adenosine triphosphate (ATP) and other biomolecules responsible for cell differentiation, proliferation, and migration to induce cell proliferation and cell signaling cascades, favoring neuronal lineage commitment, although results vary across protocols. <b>Conclusion:</b> This review indicates that PBMT-based regenerative medicine seems promising and applicable for future advances in cell-based regenerative medicine and neurology. However, alongside the potentials, it also underscores challenges of PBM-based neural regeneration, calling for parameter optimization, more in vivo studies, and shifting the focus of future studies to clinical applications.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"17 ","pages":"e1"},"PeriodicalIF":1.7,"publicationDate":"2026-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13224325/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148150161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}