光生物调节疗法对提高阴囊高热成年雄性小鼠精子生成和血睾屏障完整性的影响

IF 1.4 Q3 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
Journal of lasers in medical sciences Pub Date : 2024-09-09 eCollection Date: 2024-01-01 DOI:10.34172/jlms.2024.43
Reza Soltani, Hojjat-Allah Abbaszadeh, Hamid Nazarian, Faraj Tabeie, Hassan Akbari, Ibrahim Mohammadzadeh, Azar Afshar, Fakhroddin Aghajanpour, Fatemeh Fadaei Fathabadi, Mohsen Norouzian
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引用次数: 0

摘要

导言:不健康的生活方式(如酒精、化学品和热应激)会加重男性不育症。热应激会对称为血睾屏障(BTB)的重要结构造成损害。近来,多项研究都采用了光生物调节疗法(PBMT)来提高生殖器炎症患者的精子质量。目前的研究旨在研究激光疗法如何影响阴囊受热小鼠模型的精子发生和 BTB 结构。研究方法将 30 只 8 周大的成年雄性 NMRI 小鼠分为三组:对照组、热疗组和热疗+激光 0.03 J/cm2 组。高热组动物的睾丸暴露在 43 °C 的水中,持续 20 分钟,每隔一天进行五次。然后,每隔一天对睾丸进行一次激光照射,持续 35 天,每次 3 分钟,能量密度为 0.03 J/cm2。最后对动物的精子参数、活性氧(ROS)和谷胱甘肽(GSH)水平、立体学参数和基因表达进行评估。结果研究表明,PBMT 能显著提高精子质量、生精细胞数量、睾丸体积、ROS 和 GSH 水平以及与血睾屏障相关的基因表达。结论目前,PBMT 是解决男性不育症的一种新方法,它能保持 Sertoli 细胞中 BTB 的完整性,进而支持生殖细胞的生长和特化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Impact of Photobiomodulation Therapy on Enhancing Spermatogenesis and Blood-Testis Barrier Integrity in Adult Male Mice Subjected to Scrotal Hyperthermia.

Introduction: Unhealthy lifestyle choices such as alcohol, chemicals, and heat stress can worsen male infertility. Heat stress can cause damage to the essential structure known as the blood-testis barrier (BTB). Photobiomodulation therapy (PBMT) has been employed in various studies to enhance sperm quality in individuals with genital inflammatory conditions in recent times. The current research sought to study how laser therapy affects spermatogenesis and the structure of the BTB in a mouse model of scrotal heat exposure. Methods: Thirty adult male NMRI mice, 8 weeks old, were divided into three groups: Control, Hyperthermia, and Hyperthermia+Laser 0.03 J/cm2. The animals in the hyperthermia group had their testicles exposed to water at 43 °C for 20 minutes five times every other day. Then, the testicles were exposed to laser radiation every other day for 35 days, lasting 3 minutes each time, with an energy density of 0.03 J/cm2. Animals were sacrificed, and sperm parameters, reactive oxygen species (ROS) and glutathione (GSH) levels, stereological parameters, and gene expression were assessed in the end. Results: The study showed that PBMT can significantly enhance sperm quality, quantity of spermatogenic cells, testicular volume, levels of ROS and GSH, and gene expression related to the blood-testis barrier. Conclusion: Currently, PBMT is a novel approach for addressing male infertility by preserving the integrity of the BTB in Sertoli cells, which in turn supports the growth and specialization of germ cells.

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来源期刊
Journal of lasers in medical sciences
Journal of lasers in medical sciences RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-
CiteScore
3.00
自引率
13.30%
发文量
24
期刊介绍: The "Journal of Lasers in Medical Sciences " is a scientific quarterly publication of the Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences. This journal received a scientific and research rank from the national medical publication committee. This Journal accepts original papers, review articles, case reports, brief reports, case series, photo assays, letters to the editor, and commentaries in the field of laser, or light in any fields of medicine such as the following medical specialties: -Dermatology -General and Vascular Surgery -Oncology -Cardiology -Dentistry -Urology -Rehabilitation -Ophthalmology -Otorhinolaryngology -Gynecology & Obstetrics -Internal Medicine -Orthopedics -Neurosurgery -Radiology -Pain Medicine (Algology) -Basic Sciences (Stem cell, Cellular and Molecular application and physic)
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