{"title":"Cytotoxic effect of polystyrene nanoplastics in human umbilical vein endothelial cells (HUVECs) and normal rat kidney cells (NRK52E)","authors":"Maqusood Ahamed, Mohd Javed Akhtar","doi":"10.1016/j.jksus.2024.103505","DOIUrl":"10.1016/j.jksus.2024.103505","url":null,"abstract":"<div><div>Plastics play a crucial role in nearly each aspect of societal production and everyday life, therefore making them one of the most widespread pollutants on a global scale. Because synthetic plastics are not entirely biodegradable, they often remain in the environment and fragment into micro- and nanoplastic particles. The detrimental impacts of nanoplastics on the environment and human health have received substantial worldwide interest in recent years. However, the effects of nanoplastics on human health have not yet been fully explored. Our study aimed to investigate the cytotoxic effects of polystyrene nanoplastics on two distinct mammalian cell lines: normal rat kidney cells (NRK52E) and human umbilical vein endothelial cells (HUVECs). Results showed that polystyrene nanoplastics generate cytotoxicity in both NRK52E and HUVECs in a concentration- and time-dependent manner. Additionally, polystyrene nanoplastics induced pro-oxidant levels (e.g., reactive oxygen species and hydrogen peroxide) and reduced antioxidants (glutathione content and glutathione peroxidase enzyme activity) in both types of cells. We also found that polystyrene nanoplastics cause apoptosis in both NRK52E and HUVECs, as shown by the activation of the caspase-3 enzyme and the loss of mitochondrial membrane potential. Interestingly, it was noticed that the vulnerability of HUVECs cells against polystyrene nanoplastics was a little higher than that of NRK52E cells. Also, the cell toxicity caused by polystyrene nanoplastics in NRK52E and HUVECs was effectively alleviated by co-exposure to a reactive oxygen species inhibitor, N-acetyl-cysteine. This suggests that oxidative stress could be one of the possible pathways by which polystyrene nanoplastics cause cell toxicity. The present work warrants future study to explore the toxicity mechanisms of polystyrene nanoplastics in appropriate <em>in vivo</em> models.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":"36 11","pages":"Article 103505"},"PeriodicalIF":3.7,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hesham Hussein Rassem , Mohd Hairul Bin Khamidun , Umi Fazara Md Ali , Tony Hadibarata , Nabeel Abdullah Alrabie
{"title":"Comprehensive analysis of antioxidant and antibacterial activities of water and methanol extracts of Hibiscus flower","authors":"Hesham Hussein Rassem , Mohd Hairul Bin Khamidun , Umi Fazara Md Ali , Tony Hadibarata , Nabeel Abdullah Alrabie","doi":"10.1016/j.jksus.2024.103506","DOIUrl":"10.1016/j.jksus.2024.103506","url":null,"abstract":"<div><div>This study provides a comprehensive investigation into the antioxidant and antibacterial properties of Hibiscus flower extracts, highlighting their potential for future applications in functional foods. The research addressed key challenges by employing a multi-faceted approach, including four distinct methods for evaluating antioxidant potential: DPPH and FRAP assays, reducing power, and chelating ability of Fe ions. Total phenolic content was quantified using the Folin–Ciocalteu method, while tannins and flavonoids were assessed via vanillin–HCl and aluminum chloride assays. Additional analyses included spectrophotometric measurement of total flavonols and anthocyanins. Antibacterial activity was determined using a modified agar disk diffusion method, with results indicating significant inhibition of Salmonella typhimurium and Staphylococcus aureus by both aqueous and methanolic extracts. Colorimetric analysis revealed a reduction in chroma and hue angles, while FTIR spectroscopy identified key functional groups, such as suberin, lipids, and polysaccharides. Notably, the antioxidant efficacy varied depending on the solvent used, with aqueous extracts showing higher total phenolic content (4325.12 mg GAE/100 g) compared to methanolic extracts (3487.05 mg GAE/100 g). The study concludes that Hibiscus flower extracts, rich in antioxidants like tannins and anthocyanins, possess significant potential for use as natural preservatives and colorants in food products, supporting their role in developing novel functional foods.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":"36 11","pages":"Article 103506"},"PeriodicalIF":3.7,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tong Li , Xiuzhai Chen , Shoutao Cao , Zhongqing Liu , Lei Tian , Zhengxu Gao , Mingming Sun , Hao Zong , Dequan Wang , Mohamed A. El-Sheikh , Mingfeng Yang , Peng Liu
{"title":"Integrated physiological and transcriptome analyses of the effects of water-soluble amino acid fertilizer on plant growth","authors":"Tong Li , Xiuzhai Chen , Shoutao Cao , Zhongqing Liu , Lei Tian , Zhengxu Gao , Mingming Sun , Hao Zong , Dequan Wang , Mohamed A. El-Sheikh , Mingfeng Yang , Peng Liu","doi":"10.1016/j.jksus.2024.103504","DOIUrl":"10.1016/j.jksus.2024.103504","url":null,"abstract":"<div><div>Tobacco is a key component of China’s economy, serving as a major cash crop. With traditional fertilizers reaching their maximum potential in promoting tobacco growth, the exploration of new fertilizers has emerged as a viable solution for advancing experimental sustainable development. Spraying foliar fertilizer is a key measure to improve tobacco yield and quality. This study employed a combination of field and pot experiments to investigate the effects of applying water-soluble amino acid fertilizers on the growth, development, and quality of tobacco. The results of transcriptome analysis and physiological index measurements indicate that the application of water-soluble amino acid fertilizer can enhance the area of tobacco leaves, promote photosynthesis, and improve the chemical composition of the leaves. This research determined that the optimal concentration for spraying water-soluble amino acid fertilizer is diluted 500 times. Transcriptome analysis identified a total of 6,489 differentially expressed genes (DEGs), including 3,843 genes that were up-regulated and 2,646 genes that were down-regulated. Gene Ontology (GO)<!--> <!-->analysis demonstrated that these DEGs were significantly<!--> <!-->associated<!--> <!-->with<!--> <!-->processes<!--> <!-->including<!--> <!-->cell recognition, photosynthesis,<!--> <!-->thylakoid<!--> <!-->components, calcium ion binding, and carbohydrate binding.<!--> <!-->Additionally, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis emphasized that the DEGs were largely found in pathways<!--> <!-->related to<!--> <!-->photosynthesis-antenna proteins, interactions between plants and pathogens, photosynthesis<!--> <!-->itself, phenylpropanoid biosynthesis, and<!--> <!-->plant hormone signaling. Further research revealed that a significant number of genes involved in the auxin, gibberellin, salicylic acid, and jasmonic acid signal transduction pathways exhibited varying expression patterns following the application of water-soluble amino acid fertilizers.<!--> <!-->Additionally, the expression levels of bZIP, MYB, WRKY, bHLH, and AP2/ERF transcription factors exhibited significant variations following the application of water-soluble amino acid fertilizer. These results analyze the mechanisms of action of water-soluble amino acid fertilizers, offering new effective strategies to enhance both the yield and quality of tobacco.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":"36 11","pages":"Article 103504"},"PeriodicalIF":3.7,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142578993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abdelbaset S. El-Sorogy, Khaled Al-kahtany, Talal Alharbi, Saad S. Alarifi
{"title":"Distribution patterns, health hazards, and multivariate assessment of contamination sources of As, Pb, Ni, Zn, and Fe in agricultural soils","authors":"Abdelbaset S. El-Sorogy, Khaled Al-kahtany, Talal Alharbi, Saad S. Alarifi","doi":"10.1016/j.jksus.2024.103489","DOIUrl":"10.1016/j.jksus.2024.103489","url":null,"abstract":"<div><div>Soil heavy metal contamination is a worldwide environmental concern that presents considerable risks to ecosystems, agricultural progress, and human health. This study aims to evaluate the potential environmental and health hazards linked to the presence of arsenic (As), lead (Pb), nickel (Ni), zinc (Zn), and iron (Fe) in agricultural soil in Al Majma’ah governorate, Saudi Arabia. The contamination factor (CF), pollutant load index (PLI), chronic daily intake (CDI), hazard index (HI), and total lifetime cancer risk (LCR) were calculated for 34 soil samples. The results from the CF and PLI analysis demonstrate that the examined soil has a low contamination factor and is free from heavy metal pollution. The average CDI values for adults and children exhibited the following descending order: Fe > Zn > Cu > Pb > As. The highest HI values observed in adults ranged from 0.0375 (Fe) to 0.00019 (Zn), but in children, the range was from 0.3497 (Fe) to 0.0018 (Zn). The hazard index values for heavy metal(loid)s (HMs) in the Al Majma’ah area were all below 1.0, suggesting that residents in the area are not exposed to a significant non-carcinogenic risk. The LCR values ranged from 8.37E−06 to 7.80E-05 for As in both adults and children, and from 7.50E−08 to 6.98E-07 for Pb. The findings indicated a level of risk that was deemed acceptable or tolerable, without any significant adverse health effects.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":"36 11","pages":"Article 103489"},"PeriodicalIF":3.7,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Seed inoculation with Rhizobium japonium bacteria improved fatty acid composition of different soybean (Glycine max L.) genotypes","authors":"Aynur Bilmez Özçınar","doi":"10.1016/j.jksus.2024.103492","DOIUrl":"10.1016/j.jksus.2024.103492","url":null,"abstract":"<div><h3>Background</h3><div>Soybean is an important crop for food security as it fulfills global oil requirements. Seed inoculation with bacteria is frequently used to increase its production; however, it could change the seed composition. Nevertheless, the genotypes respond differently to the bacteria. Therefore, it is necessary to assess the impact of <em>Rhizobium</em> bacteria on the seed composition.</div></div><div><h3>Methods</h3><div>This two-year (2018 and 2019) study investigated the effects of seed inoculation with <em>Rhizobium japonicum</em> on fatty acid composition of different soybean genotypes. Three frequently cultivated soybean genotypes, i.e., ‘Gapsoy16′, ‘Traksoy’, and ‘İlksoy’ were included in the study. The seeds were either inoculated with <em>R. japonicum</em> or sown without inoculation. The fatty acid profile, i.e., saturated fatty acids (palmitic and stearic acid) and unsaturated fatty acids (oleic, linoleic, linolenic, and arachidic acids) was determined, and the collected data were analyzed by single and multivariate analysis.</div></div><div><h3>Results</h3><div>Seed inoculation with <em>R. japonicum</em> significantly altered the fatty acid composition of different genotypes; however, varied effects were recorded for the genotype. Linoleic acid, oleic acid, and palmitic acid made up ∼ 33 % of total fatty acids in seeds. Linoleic acid contents varied between 30.78–34.02 %, whereas oleic acid contents ranged between 27.85–31.04 %. Similarly, palmitic acid contents differed between 15.53–16.93 %. The ‘İlksoy’ and ‘Gapsoy’ had the highest contents of palmitic and oleic acids, respectively. Overall, inoculation of bacteria increased the composition of unsaturated fatty acids and lowered saturated fatty acids.</div></div><div><h3>Conclusion</h3><div>Seed inoculation with <em>R. japonicum</em> increased the essential fatty acid composition in ‘Traksoy’ genotype. However, ‘İlksoy’ genotype recorded a decrease in unsaturated fatty acids. Therefore, ‘Traksoy’ can be inoculated with <em>R. japonicum</em> to improve fatty acid profile.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":"36 11","pages":"Article 103492"},"PeriodicalIF":3.7,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142551956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kirill Azarin , Alexander Usatov , Tatiana Minkina , Ilya Alliluev , Nadezhda Duplii , Saglara Mandzhieva , Abhishek Singh , Vishnu D. Rajput , Sandeep Kumar , Marwa A. Fakhr , Mohamed S. Elshikh , M. Ajmal Ali , Karen Ghazaryan
{"title":"Impact nano- and micro- form of CdO on barley growth and oxidative stress response","authors":"Kirill Azarin , Alexander Usatov , Tatiana Minkina , Ilya Alliluev , Nadezhda Duplii , Saglara Mandzhieva , Abhishek Singh , Vishnu D. Rajput , Sandeep Kumar , Marwa A. Fakhr , Mohamed S. Elshikh , M. Ajmal Ali , Karen Ghazaryan","doi":"10.1016/j.jksus.2024.103493","DOIUrl":"10.1016/j.jksus.2024.103493","url":null,"abstract":"<div><div>The objective was investigated the effects of CdO and nano-CdO as potential toxic pollutants on growth and redox response of barley. CdO and nano-CdO have been found to cause significant phytotoxicity in barley seedlings, with nano-CdO increasing plant tissue cadmium accumulation. This accumulation is linked to growth retardation and oxidative stress. Low molecular weight antioxidants like restored glutathione and ascorbate have been found to increase the activity of glutathione peroxidase (GPX), glutathione-S-transferase (GSTs), and ascorbate peroxidase (APX) in green tissues. Catalase (CAT) activity increased from 50 % with 100 mg/l CdO to 70 % with 1000 mg/l and nano-CdO. The observed disturbance in redox balance signals the upregulation of corresponding genes. Antioxidant enzyme isoform gene transcripts increased for <em>SODB, CAT2,</em> and <em>APX</em>. Cadmium buildup in root cells causes oxidative stress, leading to upregulation of SOD, CAT, GR, and GSTs isoform genes as well as protein carbonylation, sulfhydryl group degradation, and MDA accumulation. CdO and nano-CdO have similar phytotoxic effects, but bioavailability affects biochemical and molecular responses.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":"36 11","pages":"Article 103493"},"PeriodicalIF":3.7,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abdullah M Alhadheq , Rana Alhadheq , Saad Alkahtani
{"title":"Aberrant expression of miR-143/miR-223/miR4478 and miR145 as prognostic factor for colorectal cancer patients","authors":"Abdullah M Alhadheq , Rana Alhadheq , Saad Alkahtani","doi":"10.1016/j.jksus.2024.103494","DOIUrl":"10.1016/j.jksus.2024.103494","url":null,"abstract":"<div><div>Colorectal cancer (CRC) is a type of malignancy that develops in the colon or rectal region. It develops due to abnormal growth and proliferation of cells in the lining of the colon, forming a tumor. This study included tissue samples (56 malignant and 56 matched normal samples). miRNAs were determined and retrieved from miRBase database and moderated by p53 gene. Expression levels of miRNA were varied. Only miR-223 revealed greater expression than normal matched tissue. mMiR-143, miR-4478, and miR-145 exhibited lower expression than matched normal tissue. Only miR-143 and miR-145 showed considerable variations in the expression among groups while miR-4478 did not demonstrate statistically substantial difference. In conclusion, this study highlights that upregulation of P53 and miR-223 and downregulation of miR-145 and miR-143 were associated with cancer advancement and unfavorable prognosis in Saudi CRC patients, indicating P53, miR-223, miR-145 and miR-143 to be novel and valuable signatures for predicting the outcomes for patients with CRC.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":"36 11","pages":"Article 103494"},"PeriodicalIF":3.7,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anas Charbaoui , Mourad Guernouche , Azzouz Kchikach , Mohammed Jaffal , Abdessamad Hadri , Mihai Octavian Cimpoiasu , Samuel Zappalá , Mounir Amar , Oussama Khadiri Yazami
{"title":"Understanding the impact of deep structures on the hydrological setting of the Eastern Bahira Basin in Morocco using combined geophysical analysis of gravity, seismic, and electrical resistivity data","authors":"Anas Charbaoui , Mourad Guernouche , Azzouz Kchikach , Mohammed Jaffal , Abdessamad Hadri , Mihai Octavian Cimpoiasu , Samuel Zappalá , Mounir Amar , Oussama Khadiri Yazami","doi":"10.1016/j.jksus.2024.103491","DOIUrl":"10.1016/j.jksus.2024.103491","url":null,"abstract":"<div><div>In arid regions like the Eastern Bahira Basin, communities mainly rely on groundwater for drinking and irrigation. However, efficiently managing these vital resources requires a deep understanding of the underlying aquifers’ structure and identifying the most suitable areas for exploitation. This presents a significant challenge for the success of water supply and irrigation programs in the Eastern Bahira Basin. This study is based on an integrative approach, combining Electrical Resistivity Tomography data, with the compilation and reinterpretation of pre-existing seismic, gravimetric and vertical electrical sounding data. This approach is based on compiling old gravimetric data and applying advanced processing techniques to determine the horizontal gradient maxima, which helps highlight the major structural alignments in the basin. Furthermore, the approach utilizes seismic data in order to enhance understanding of the deep structure of the basin, reinterpreting it in light of recent drilling data. The interpretation of the gravimetric and seismic data has also been validated by the results of vertical electrical soundings and electrical tomography that we recently acquired in the Eastern Bahira basin. The outcomes of this research provide new insights into the deep structure of the Eastern Bahira Basin and suggest the most promising hydrogeological prospects, thereby contributing to the success of the ongoing drinking water supply and irrigation program in the Eastern Bahira Basin.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":"36 11","pages":"Article 103491"},"PeriodicalIF":3.7,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hanaa M. Hassan , Hanaa S.S. Gazwi , Inas Hussein Refaat , Asmaa Hussein Zaki , Marija Milošević , Salim S. Al-Rejaie , Mohamed Mohany , Eman E. Yassien
{"title":"Effects of Laurus extract against cadmium-induced neurotoxicity in rats","authors":"Hanaa M. Hassan , Hanaa S.S. Gazwi , Inas Hussein Refaat , Asmaa Hussein Zaki , Marija Milošević , Salim S. Al-Rejaie , Mohamed Mohany , Eman E. Yassien","doi":"10.1016/j.jksus.2024.103488","DOIUrl":"10.1016/j.jksus.2024.103488","url":null,"abstract":"<div><div>Cadmium (Cd) is a hazardous heavy metal with widespread environmental presence, posing significant threats to human and animal health. This study investigates the neurotoxic effects of Cd exposure and explores the therapeutic efficacy of <em>Laurus nobilis</em> L. in counteracting these adverse effects. Cd exposure induces oxidative stress and neurotoxicity, leading to neuronal damage and histopathological alterations. <em>Laurus</em>, known for its antioxidant properties, is assessed for its potential in mitigating Cd-induced neurotoxicity through in vitro antioxidant assays, GC–MS analysis, HPLC profiling, and experimental animal models. Results demonstrate that <em>Laurus</em> ethanolic extract exhibits significant antioxidant activity, attributed to its phenolic and flavonoid constituents. GC–MS analysis reveals various bioactive compounds in the extract, including Hexadecanoic acid methyl ester and (Z)-13-Docosenamide, which posses neuroprotective properties. HPLC analysis identifies phenolic compounds such as ellagic acid and flavonoids like rutin and kaempferol in the extract. In vivo studies in rats exposed to Cd demonstrate that <em>Laurus</em> extract administration mitigates Cd-induced alterations in body and brain weight, hematological parameters, liver and kidney function, oxidative stress markers, and acetylcholinesterase (AchE) activity. Histopathological examination confirms the protective effects of <em>Laurus</em> against Cd-induced neuronal damage. The SOD model, validated with high resolution (2.05 Å) and strong R values (work: 0.192, free: 0.236, observed: 0.194), shows strong structural stability (C-score: 0.30). Docking studies reveal high binding affinities of kaempferol (−8.1 kcal/mol) and rutin (−8.7 kcal/mol) with SOD with kaempferol demonstrating superior solubility, lipophilicity, and drug-likenessSimulation analysis confirms the protein’s flexibility and adaptability, highlighting its therapeutic potential, especially with kaempferol. These findings suggest the therapeutic potential of <em>Laurus</em>. as a natural remedy for Cd-induced neurotoxicity, highlighting its antioxidant and neuroprotective properties.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":"36 11","pages":"Article 103488"},"PeriodicalIF":3.7,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chinnadurai Veeramani, Mohammed A. Alsaif, Muhammad Ibrar Khan, Ahmed S. El Newehy, Ali Alshammari, Khalid S. Al-Numair
{"title":"Calcium nanoparticles produced by Acacia arabica leaf extract and their influence on fresh-cut fruit quality features","authors":"Chinnadurai Veeramani, Mohammed A. Alsaif, Muhammad Ibrar Khan, Ahmed S. El Newehy, Ali Alshammari, Khalid S. Al-Numair","doi":"10.1016/j.jksus.2024.103487","DOIUrl":"10.1016/j.jksus.2024.103487","url":null,"abstract":"<div><div>Calcium oxide nanoparticles have possessed unique structural and numerous applications, including the food preservative, antimicrobial, and chemotherapeutic properties. The study’s goal was to identify and create calcium oxide-derived leaf extract nanoparticles from <em>Acacia arabica</em> (AA) and assess their effectiveness on fresh-cut fruit quality attributes. The calcium oxide nanoparticles (AACaN) produced using AA leaf extract by the simple precipitation method and the AACaN obtained were characterised and confirmed using various analytical techniques. Freshly sliced apple fruit as well as unsliced blueberry and blackberry were immersed in AACaN (10 and 20 µg/mL) at two different concentrations for five minutes before being put in packed polypropylene plastic bags and maintained at 5 °C. According to the quality evaluations of pH, DPPH, 2,2-diphenylpicrylhydrazyl (DPPH), hardness, total soluble solid content (TSS), and sensory analysis. AACaN at 20 µg/mL has the ability to preserve freshly cut fruits of apples as well as unsliced blueberries and blackberries, thereby effectively extending fruits 20-day shelf life in comparison to control (untreated) fruits. This study has concluded that the AA is an ideal source to synthesis calcium oxide nanoparticles, and it was proved by materially characterizing. Moreover, the AACaN at 20 µg/mL proved the apples, blueberry, and blackberry fruits shelf life, which also added a large nutritional value while maintaining a storage quality.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":"36 11","pages":"Article 103487"},"PeriodicalIF":3.7,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}