Journal of Inflammation Research最新文献

{"title":"Exportin-T Promotes Breast Cancer Progression via PI3K/AKT/mTOR Signaling Pathway.","authors":"Zhichao Hou, Wenxia Ma, Dongliang Ren, Ningning Shen, Weilin Bi, Meiqin Guo, Xinzheng Li, Yanhong Wang, Hongyan Jia","doi":"10.2147/JIR.S512905","DOIUrl":"10.2147/JIR.S512905","url":null,"abstract":"<p><strong>Purpose: </strong>Breast cancer (BC) is the most common malignant tumor in women. Exportin-T (XPOT) which is a member of the karyopherin -β family has been identified as a prognostic biomarker in various cancers, but its role in BC remains inadequately understood. This study aims to investigate the clinical characterization and molecular mechanism of XPOT in BC.</p><p><strong>Material and methods: </strong>A retrospective RNA-seq data analysis based on a cohort of 966 BC patients from The Cancer Genome Atlas database (TCGA) and 1904 patients from the Molecular Taxonomy of Breast Cancer International Consortium database was conducted for analyzing the correlation between XPOT expression and BC clinical pathological features. In addition, small interfering RNA transfection was used to downregulate XPOT expression in MDA-MB-468/231 cell lines followed by cell proliferation assessed via Cell Counting Kit-8 assays, meanwhile, BC cell migration and invasion capabilities were measured using Transwell test. Expression levels of CDK4/6 and key proteins in the PI3K/Akt/mTOR signaling pathway were assessed using Western blotting.</p><p><strong>Results: </strong>We found that XPOT was enriched in estrogen receptor (ER)-negative, progesterone receptor (PR)-negative, larger tumor size, and cases with increased lymph node metastasis BC. XPOT was identified as a potential biomarker for the basal subtype of BC and a prognostic factor for the overall survival of patients with BC. Furthermore, XPOT promoted the proliferation and invasion of BC cells, likely through activation of the PI3K/AKT/mTOR signaling pathway, which in turn to upregulate cyclin D and CDK4/6 to drive tumor progression.</p><p><strong>Conclusion: </strong>Our findings indicate that XPOT overexpression is associated with poor clinical characteristics and poor prognosis in BC, promoting disease progression by activating PI3K/AKT/mTOR pathway. These findings highlight XPOT as a potential therapeutic target in BC.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"6467-6481"},"PeriodicalIF":4.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12103871/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Association Between Neonatal Respiratory Distress Syndrome and Plasma IgG N-Glycosylation: A Case-Control Study.","authors":"Yingjie Wang, Qingqing Shen, Ruxu Yan, Meng Wang, Min Xu, Hanxiang Chen, Dong Li","doi":"10.2147/JIR.S524188","DOIUrl":"10.2147/JIR.S524188","url":null,"abstract":"<p><strong>Background: </strong>Neonatal respiratory distress syndrome (NRDS) is the leading cause of neonatal death. Changes in plasma immunoglobulin G (IgG) N-glycosylation have been demonstrated in a variety of diseases. However, its implications and clinical significance in NRDS remain to be clarified.</p><p><strong>Methods: </strong>To determine the effect of IgG N-glycosylation on NRDS, we recruited 88 NRDS participants and 120 control participants from December 2021 to September 2022. Plasma was collected, IgG was isolated and purified, and the glycogram was analyzed by ultra performance liquid chromatography (UPLC) with fluorescence detector.</p><p><strong>Results: </strong>The occurrence of premature rupture of membranes (PROM) [OR=9.043(1.036-78.966), P=0.046] and the elevation of γ-glutamyltransferase (GGT) [OR=1.015(1.001-1.029), P=0.032] were independent risk factors for the occurrence of NRDS. Furthermore, the area percentages of GP1, GP3, GP4, GP11, GP13, and GP24 were significantly higher in NRDS patients compared with control group. Conversely, GP14 was observed to be significantly lower. Furthermore, an increase in plasma IgG sialylation and core fucosylation was observed in NRDS, whereas the modification with galactosylation was decreased. The model constructed using GP1, GP13, GP14, PROM, and GGT as composite indices demonstrated robust predictive performance (AUC=0.902, 95% CI: 0.851-0.953).</p><p><strong>Conclusion: </strong>Patients with NRDS frequently exhibit alterations in the glycosylation of plasma IgG. These findings provide new insights into the diagnosis of NRDS and clinical treatment.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"6439-6451"},"PeriodicalIF":4.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12103862/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying KL-6-Associated Immune Cell Signatures and Key Genes in Emphysematous COPD.","authors":"Xinru Xiao, Wenwen Guo, Na Li, Nuo Chen, Qian Zhang","doi":"10.2147/JIR.S515653","DOIUrl":"10.2147/JIR.S515653","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to evaluate the potential of Krebs von den lungen-6 (KL-6) as a biomarker for distinguishing emphysematous chronic obstructive pulmonary disease (COPD-E) from non-emphysematous COPD (COPD-NE), and to explore the underlying mechanisms associated with KL-6 expression.</p><p><strong>Methods: </strong>We enrolled 154 patients with COPD and 170 healthy controls to assess serum KL-6 levels. Receiver operating characteristic curve was used to determine the diagnostic sensitivity and specificity. Pearson's correlation analysis was used to evaluate the correlation. Univariate and multivariate linear regression analyses were performed to explore the factors influencing KL-6 levels in COPD. Transcriptomic sequencing was performed on peripheral blood mononuclear cells from COPD patients with varying KL-6 levels to explore underlying biological mechanisms. A Mendelian randomization analysis was employed to ascertain the association between the expression quantitative trait loci of key genes and emphysema risk.</p><p><strong>Results: </strong>Serum KL-6 levels were significantly elevated in COPD patients, particularly in COPD-E. Pearson analyses revealed that the serum KL-6 concentration was positively correlated with eosinophil count. Transcriptomic analysis revealed 237 differentially expressed genes (DEGs) between patients with high and low levels of KL-6. Gene set enrichment analysis revealed that these DEGs were associated with immune responses. No significant difference in immune cell proportions were observed between high and low KL-6 groups, but KL-6 showed a negative correlation with T cell gamma delta. By intersecting the DEGs with those from the GSE248493 dataset, we identified seven key genes and further validated their association with the risk of emphysema using Mendelian randomization, with amidohydrolase domain containing 2 (AMDHD2) potentially reducing the risk of the disease.</p><p><strong>Conclusion: </strong>KL-6 is a promising biomarker for distinguishing COPD-E from COPD-NE and AMDHD2 may be involved in the regulation of increased KL-6 levels in COPD-E.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"6453-6466"},"PeriodicalIF":4.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12103879/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Inhibitory Effect of the Active Ingredients in the Bushen Huoxue Formula on the IL-17A Signaling Pathway and Its Alleviating Effect on Osteoarthritis.","authors":"Xuan Wang, Yunheng Zhang, Xin Chang, Xiaodong Wen, Feng Tian, Hanjie Yu, Yi Li","doi":"10.2147/JIR.S506716","DOIUrl":"10.2147/JIR.S506716","url":null,"abstract":"<p><strong>Objective: </strong>Osteoarthritis (OA) stands as a prevalent degenerative disease worldwide. Despite the demonstrated therapeutic efficacy of the Bushen Huoxue formula (BSHXF) in treating OA, its underlying mechanism remains elusive. Network pharmacology is commonly employed for investigating drug-disease associations and processes. In this study, we employed network pharmacology alongside in vitro and in vivo experiments to elucidate the molecular mechanism by which BSHXF treats OA.</p><p><strong>Methods: </strong>Based on the TCMSP database, active components of BSHXF were screened, and OA-related targets were retrieved from GeneCard and DisGeNET to construct a \"component-target-pathway\" network using Cytoscape. Core target functions and pathways (KEGG/GO) were analyzed through STRING and Metascape, while component-target binding affinity was validated via Autodock. For in vitro experiments, an IL-1β-induced chondrocyte inflammation model was established, and key protein expression was detected by Western blot and immunofluorescence. For in vivo experiments, an OA model was created by medial meniscectomy of the knee joint in rats, and therapeutic efficacy was assessed using histological staining and micro-CT.</p><p><strong>Results: </strong>This study screened 89 active ingredients of BSHXF and identified 189 common targets. Network pharmacological analysis revealed luteolin and tanshinone IIA as the most crucial active ingredients in treating OA with BSHXF. The potential mechanisms of action for BSHXF in OA treatment involve inflammation inhibition, immune function regulation, and resistance to oxidative stress, with a significant regulatory role played by the IL-17 signaling pathway. Molecular docking results demonstrated luteolin's strong binding affinity to key targets such as B-cell lymphoma 2 (Bcl-2), Matrix metalloproteinase-9 (Mmp-9), and IL-6.In vitro experiments demonstrated that BSHXF significantly suppressed IL-1β-induced inflammatory responses in chondrocytes, downregulating IL-17A expression (<i>p</i> < 0.05), reducing the expression of MMP-9 (<i>p</i> < 0.05) and IL-6 (<i>p</i> < 0.05), and inhibiting apoptosis. Additionally, in vivo experiments revealed that the high-dose BSHXF group (150 mg/kg) markedly alleviated cartilage damage in OA rats, with OARSI scores significantly decreased compared to the model group (<i>p</i> < 0.05). Micro-CT analysis showed that BSHXF inhibited osteophyte formation and ameliorated OA pathological conditions.</p><p><strong>Conclusion: </strong>BSHXF has the potential to alleviate OA by suppressing inflammation, inhibiting cartilage apoptosis and hindering extracellular matrix degradation via the IL-17 signaling pathway. Our study elucidated the molecular mechanisms underlying the therapeutic effects of BSHXF on OA, thus highlighting its further research implications as a novel drug candidate.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"6505-6527"},"PeriodicalIF":4.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12104672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Characteristics and Mechanism Discussion of Peripheral Nerve Injury in 2 Cases of Severe Viral Meningoencephalitis.","authors":"Xiaosu Guo, Mengyi Zheng, Zibin Wei, Jianghua Song, Xue Wang, Zhiyuan Shen, Xin Guo, Nan Zhang, Yuan Xing, Yaxin Zhang, Wei Zhang, Runxuan Du, Bo Qiu, Shujuan Tian, Zhiwei Wang","doi":"10.2147/JIR.S505159","DOIUrl":"10.2147/JIR.S505159","url":null,"abstract":"<p><strong>Purpose: </strong>Peripheral neuropathy(PN) secondary to central nervous system(CNS) infections is rare in clinical practice. This study analyze the prognosis, clinical characteristics, and outcomes of patients with PN secondary to CNS infections to aid early diagnosis and improve prognosis.</p><p><strong>Methods: </strong>Clinical data from two patients admitted to our Neurology Department with PN secondary to severe viral meningoencephalitis were collected, summarized, and analyzed. Using diagnostic tools like body fluid tests, imaging, EEG, and EMG, and based on the criteria of the International Encephalitis Consortium, encephalitis was diagnosed in Case 1 and Case 2. The European Academy of Neurology/Peripheral Nerve Society recommendations were applied to confirm patients' PN diagnosis.</p><p><strong>Results: </strong>Patient 1 was diagnosed with encephalitis, presenting with elevated serum IL-6 levels, and received IVIG treatment upon admission. One week later, the infection remitted and IL-6 levels decreased. Physical and EMG examinations revealed peripheral nerve demyelination damage. After treatment, the nerve damage improved, and the patient had a good prognosis post-discharge. Upon admission, Patient 2 exhibited viral meningoencephalitis symptoms, with elevated serum IL-8 and normal IL-6 levels; limb muscle strength and tone were normal. Five days later, the infection deteriorated, accompanied by reduced lower limb strength, and elevated IL-6 and IL-8 in serum and CSF, with a striking peak of CSF IL-6. EMG confirmed peripheral nerve demyelination and axonal damage. Following 5-day IVIG treatment, IL-6 and IL-8 levels in serum and CSF declined. Peripheral nerve injury recovery was modest despite treatment, and the patient's prognosis remained moderate.</p><p><strong>Conclusion: </strong>This study reported two rare cases of PN following CNS infection. Comparative analysis of symptoms, cytokine in body fluids, treatments, disease courses, and prognosis indicates that elevated peripheral and/or central cytokines, particularly IL-6 and IL-8, correlate with the severity and prognosis of this complication. IVIG modulates inflammation, and its administration timing likely determines differential outcomes.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"6397-6410"},"PeriodicalIF":4.2,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12103168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pan-Immune-Inflammation Value as a Prognostic Biomarker for Hepatocellular Carcinoma Patients Undergoing Hepatectomy.","authors":"Hongyuan Fu, Yubo Wang, Bangde Xiang","doi":"10.2147/JIR.S521603","DOIUrl":"10.2147/JIR.S521603","url":null,"abstract":"<p><strong>Purpose: </strong>Hepatocellular carcinoma (HCC) poses a substantial threat to global health, characterized by its high incidence and mortality rates. This research aims to assess the prognostic value of a systematic serum inflammation index, the pan-immune-inflammation value (PIV), in patients with HCC who have undergone hepatectomy.</p><p><strong>Patients and methods: </strong>A total of 1764 HCC patients who underwent surgery were included in the study. These patients were divided into two groups based on the median PIV value. The Cox regression model was utilized to ascertain the independent risk factors that influence the prognosis of patients. A PIV-based nomogram was constructed and its performance was evaluated by the C-index, calibration curve, ROC curve, and DCA curve. Finally, a comparison was made between the nomogram and existing staging models.</p><p><strong>Results: </strong>Patients with elevated PIV exhibited diminished OS and RFS compared to those with lower PIV. Univariate and multivariate Cox analyses revealed that PIV is an independent predictor of prognosis. The PIV-based nomogram demonstrated excellent discrimination, calibration, and clinical net benefit. The proposed nomogram outperformed the other existing staging systems, as evidenced by a higher AUC value.</p><p><strong>Conclusion: </strong>PIV exhibits potential as a prognostic factor for both OS and RFS in patients with HCC who have undergone hepatectomy. The PIV-based nomogram can serve as an additional tool in conjunction with the existing liver cancer staging system, thereby facilitating more personalized treatment decisions for clinicians.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"6411-6425"},"PeriodicalIF":4.2,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12103170/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine Learning-Based Mortality Risk Prediction Model in Patients with Sepsis.","authors":"Ye Zhang, Chen Li, Yilin Ji, Bing Wei, Shubin Guo, Xue Mei, Junyu Wang","doi":"10.2147/JIR.S502837","DOIUrl":"10.2147/JIR.S502837","url":null,"abstract":"<p><strong>Objective: </strong>The aim of our study was to establish and validate a machine learning-based predictive model for mortality risk in elderly patients with sepsis. By integrating traditional biomarkers, novel biomarkers, clinical data, and established scoring systems, the model seeks to enhance predictive accuracy and thereby improve clinical outcomes in high-risk patient population.</p><p><strong>Methods: </strong>Conducted at Beijing Chao-Yang Hospital from August 2021 to August 2023, our study included 180 emergency department patients meeting Sepsis 3.0 diagnostic criteria. Data collected included patient demographics, vital signs, laboratory parameters, disease-related scores, major comorbidities, and the 28-day mortality. Variables were analyzed using univariate analysis and LASSO regression, and the machine learning model was constructed using R statistical software and validated internally via bootstrap resampling and calibration curves.</p><p><strong>Results: </strong>The model identified seven significant variables: SOFA, APACHE II, MAP, ALB, PCT, LTB, and VEGF. These variables constituted our final prediction model, which achieved an AUC of 0.845 (95% CI: 0.786, 0.905), with a sensitivity of 75.9% and a specificity of 85.0%. Internal validation yielded a bootstrap-corrected AUC of 0.857 (95% CI: 0.799, 0.912), confirming the model's statistical robustness. The nomogram provided a visual tool for predicting 28-day mortality risk, and decision curve analysis demonstrated strong potential for clinical utility.</p><p><strong>Conclusion: </strong>The predictive model, which incorporates SOFA, APACHE II, MAP, ALB, PCT, LTB, and VEGF, shows significant potential in predicting the 28-day mortality risk for elderly sepsis patients. It provides a convenient and rapid tool for clinical use. Further research with larger sample sizes and external validation is warranted to confirm these findings and enhance the model's applicability.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"6427-6437"},"PeriodicalIF":4.2,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101463/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>CLEC3B</i> as a Prognostic and Immunological Biomarker in Pan-Cancer: Multi-Omics Profiling and Validation in Pancreatic Cancer and Exosomes.","authors":"Kai Nan, Lei Zhang, Yulong Peng, Jing Huang, Su Yin, Yujia Zou, Kaikai He, Ming Zhang","doi":"10.2147/JIR.S517595","DOIUrl":"10.2147/JIR.S517595","url":null,"abstract":"<p><strong>Background: </strong>Despite the emergence of in vitro and in vivo experiments validating the connection between <i>CLEC3B</i> and various cancers, a comprehensive pan-cancer investigation remains elusive. In this study, we explored the potential roles of <i>CLEC3B</i> as a tumor suppressor and in immune function across multiple cancer types.</p><p><strong>Methods: </strong>We visualized outcomes derived from Gene Expression Omnibus (GEO) and diverse online databases. The relationship between tumor-infiltrating cells, gene set enrichment analysis (GSEA) and CLEC3B expression and was examined using R. Additionally, we explored the potential role of CLEC3B in tumor malignant behavior by using siRNA-mediated knockdown.</p><p><strong>Results: </strong>Our study identifies <i>CLEC3B</i>'s low expression in majority of cancers compared with adjacent normal tissues. Reduced <i>CLEC3B</i> expression correlated with advanced clinical stages, inferior overall survival (OS) and DNA methylation levels. We observed significant positive associations between <i>CLEC3B</i> expression and infiltration levels of various immune cell subtypes. Furthermore, markers linked with immune checkpoints, immunomodulation and RNA modification exhibited a favorable correlation with <i>CLEC3B</i> expression. Intriguingly, silencing <i>CLEC3B</i> (si-<i>CLEC3B</i>) augmented the migratory capabilities of pancreatic adenocarcinoma (PAAD) cells. Additionally, <i>CLEC3B</i> expression was notably enriched in metastatic PAAD endothelial cells and extracellular vesicles, potentially implicating its involvement in tumor vascular function by way of extracellular vesicle.</p><p><strong>Conclusion: </strong>In conclusion, our initial pan-cancer analyses of <i>CLEC3B</i> provide insights into its associations with clinical prognosis, DNA methylation, immune cell infiltration, and tumor mutation burden, highlighting its potential as a tumor suppressor and mediator of immune infiltration in pan-cancer.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"6381-6396"},"PeriodicalIF":4.2,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101469/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Fatty Acid Metabolism Disorder-Related Gene Signature in Septic Cardiomyopathy.","authors":"Liman Li, Tiancong Zhang, Chuan Yang, Qiang Meng, Shuang Wang, Yang Fu","doi":"10.2147/JIR.S477110","DOIUrl":"10.2147/JIR.S477110","url":null,"abstract":"<p><strong>Background: </strong>Septic cardiomyopathy (SCM) is a prevalent complication of sepsis and a primary contributor to mortality in patients with sepsis. Although fatty acid metabolism (FAM) is known to regulate cardiac function, its specific role in the pathogenesis of SCM remains unclear.</p><p><strong>Methods: </strong>The SCM datasets were obtained from the NCBI GEO database. Differentially expressed genes (DEGs) were subjected to GO and KEGG pathway analyses. The fatty acid metabolism-related genes were obtained from the MSigDB database. CytoHubba and machine learning algorithms identified hub FAM-DEGs. Associated transcriptional factors and miRNAs of hub FAM-DEGs were predicted using Cytoscape software and miRWalk 3.0 database. The immune infiltration pattern in SCM was analyzed using the ImmuCellAI tool. The relationship between hub FAM-DEGs and immune infiltration abundance was investigated using Spearman method. Hub FAM-DEGs expression levels were validated in clinical samples and mouse models.</p><p><strong>Results: </strong>Five hub FAM-DEGs associated with SCM were identified, including <i>Hsd17b7, Dhcr24, Cyp1a1, Ephx1</i> and <i>Hmgcs2</i>. Immune analysis revealed significantly increased infiltrations of granulocytes, monocytes, M1 macrophage and neutrophils in the SCM group. Spearman analysis demonstrated that the hub FAM-DEGs were positively associated with the infiltration of pro-inflammatory immune cells. In Vivo, Down-regulations of Dhcr24 mRNA and protein levels in cardiac tissues were observed in the SCM mouse group. Clinically, the plasma concentration of DHCR24 was significantly decreased in patients with SCM.</p><p><strong>Conclusion: </strong>This study revealed fatty acid metabolism played a crucial role in SCM and identified DHCR24 may act as a potential diagnostic biomarker and therapeutic target in SCM.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"6363-6380"},"PeriodicalIF":4.2,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12096332/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends in Research on the P2X7 Receptor: A Bibliometric and Visualization Analysis.","authors":"Chao Ren, Jingjing Zhang, Ting Yang, Yao Wang, Xiaoyu Song, Hanrui Wang, Wanchen Liu, Yakui Mou, Xicheng Song","doi":"10.2147/JIR.S522380","DOIUrl":"10.2147/JIR.S522380","url":null,"abstract":"<p><p>The P2X7 receptor (P2X7R), activated by ATP, participates in mediating a variety of biological processes such as the release of inflammatory factors, thereby affecting the pathogenesis of numerous diseases. Despite its clinical significance, comprehensive bibliometric studies on P2X7R-related research remain scarce. To address this gap, we conducted a bibliometric investigation to quantify publication outputs, identify leading contributors, and map geographical distributions of P2X7R-related research, ultimately elucidating current research frontiers and emerging trends. Utilizing visualization techniques, complex bibliometric relationships were transformed into interpretable graphical representations. Our Web of Science query for \"P2X7R\" identified 4551 original research articles by 13,471 authors published since 1985. Publication volume demonstrated sustained growth, with China emerging as the predominant contributor in quantitative output (1871 articles) and the United States leading in international collaborations. Thematic mapping revealed two principal research areas: \"P2X7R, inflammation, microglia\" and \"P2X7R, apoptosis, purinergic\". Notably, \"P2X7R, P2 receptors, oxidative stress\" encompassed both motor and niche themes, thus representing the trajectory of future development potential. Through bibliometric and visualization analysis, we found that the literature related to P2X7R is on the rise and will enter a new stage of interdisciplinary integration development in the future.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"6349-6362"},"PeriodicalIF":4.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091240/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}