Drug Testing and Analysis最新文献_第7页

An Improved GC-MS/MS Method for a Fast Multidrug Analysis in Hair. 头发中多药快速分析的改进GC-MS/MS方法。

IF 2.6 3区医学

Drug Testing and Analysis Pub Date : 2024-12-14 DOI: 10.1002/dta.3840

HuiFen Hannah Zhang, Jia Ying Hilda Loh, Hooi Yan Moy, Chi Pang Lui

{"title":"An Improved GC-MS/MS Method for a Fast Multidrug Analysis in Hair.","authors":"HuiFen Hannah Zhang, Jia Ying Hilda Loh, Hooi Yan Moy, Chi Pang Lui","doi":"10.1002/dta.3840","DOIUrl":"https://doi.org/10.1002/dta.3840","url":null,"abstract":"This study presents the development and validation of a drug testing method in hair, employing a semi-automated sample preparation procedure and gas chromatography-tandem mass spectrometry (GC-MS/MS) for simultaneous multidrug analysis in hair. The method aims to detect and quantify multiple drugs in hair, including amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, ketamine, norketamine, cocaine, benzoylecgonine, morphine, codeine and 6-monoacetylmorphine, using a comprehensive procedure involving decontamination, pulverization, clean-up using supported-liquid extraction (SLE) and a two-step derivatization process. The method validation included specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, carryover, robustness and stability. The results indicate a linearity range of 0.1-5 ng/mg for all drugs except methamphetamine, which has a linearity range of 0.2-5 ng/mg. The accuracy and precision of the method are within ±20%, with the LOQ at 0.2 ng/mg for methamphetamine, 0.1 ng/mg for amphetamine and MDMA and 0.05 ng/mg for the rest of the drugs. The LODs were found to be 0.1 ng/mg for methamphetamine, 0.05 ng/mg for amphetamine and MDMA and 0.025 ng/mg for other drugs. This method has demonstrated its accuracy by comparing the quantitative test results with the expected results, using hair samples from the proficiency test service providers. This test method offers efficient testing of multiple drugs in a single analysis, significantly improves the hair analysis workflow in a routine testing laboratory by reducing turnaround time and the amount of hair sample required.","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Studies on the Stability and Microbial Biotransformation of Five Deschloroketamine Derivatives as Prerequisite for Wastewater-Based Epidemiology Screening. 五种去氯氯胺酮衍生物的稳定性和微生物转化研究——废水流行病学筛选的前提。

IF 2.6 3区医学

Drug Testing and Analysis Pub Date : 2024-12-11 DOI: 10.1002/dta.3839

Fabian Frankenfeld, Lea Wagmann, Markus R Meyer

{"title":"Studies on the Stability and Microbial Biotransformation of Five Deschloroketamine Derivatives as Prerequisite for Wastewater-Based Epidemiology Screening.","authors":"Fabian Frankenfeld, Lea Wagmann, Markus R Meyer","doi":"10.1002/dta.3839","DOIUrl":"https://doi.org/10.1002/dta.3839","url":null,"abstract":"Wastewater (WW)-based epidemiology (WBE) is a powerful tool for screening and surveillance of drugs (of abuse) or new psychoactive substances (NPSs) in larger population. Since the drug market changes frequently, it is crucial for WBE to define screening and surveillance biomarkers considering drug metabolism and (microbial) stability. The aims of the presented work were first to identify metabolites, potentially serving as a WBE biomarker of five deschloroketamine derivatives (DCKDs) in rat feces samples after oral administration in addition to already known urinary metabolites, and second to elucidate the microbial biotransformation and WW stability of five DCKDs and their metabolites detected in urine and feces. Microbial biotransformation and stability of DCKD and their metabolites in WW were assessed by incubating parent compounds at 0.1 mg/L or rat urine or rat feces samples in freshly collected, untreated, influent WW over a period of 24 h. All samples were analyzed using liquid chromatography-high-resolution tandem mass spectrometry. All parent compounds, seven Phase I, and one Phase II metabolite were detected in rat feces samples. After WW incubations, all tested DCKD and their metabolites were still detectable at least in trace amounts, but particularly, peak areas of the Phase II N- and O-glucuronides showed a markable decrease. This is in line with previous findings where Phase II conjugates were identified to be unstable in WW and thus not recommended as a WW biomarker. Hence, incubations demonstrated that the five DCKD and most of their metabolites were sufficiently stable in WW influent and can thus be used as analytical targets in the context of WBE.","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolism of Six Novel Nitazenes in Human Liver Microsomes Based on Ultra-High-Performance Liquid Chromatography Coupled With High-Resolution Mass Spectrometry. 基于高效液相色谱-高分辨率质谱联用的六种新型nitazene在人肝微粒体中的代谢

IF 2.6 3区医学

Drug Testing and Analysis Pub Date : 2024-12-11 DOI: 10.1002/dta.3838

Bo-Yu Huang, Zhen-Dong Hua, Cui-Mei Liu, Jing Li, Jian-Zhao Shu, Zhiyu Li

{"title":"Metabolism of Six Novel Nitazenes in Human Liver Microsomes Based on Ultra-High-Performance Liquid Chromatography Coupled With High-Resolution Mass Spectrometry.","authors":"Bo-Yu Huang, Zhen-Dong Hua, Cui-Mei Liu, Jing Li, Jian-Zhao Shu, Zhiyu Li","doi":"10.1002/dta.3838","DOIUrl":"https://doi.org/10.1002/dta.3838","url":null,"abstract":"Synthetic opioids are a chemically diverse group of substances that function as central nervous system depressants, among which the 2-benzylbenzimidazole derivatives, also known as nitazenes or nitazene analogs, have recently emerged into the recreational market. The detection of these substances in biological samples largely relies on the acquisition of metabolic data. In this study, the first-phase metabolism patterns of methylenedioxynitazene, N-desethyl etonitazene, N-desethyl methylenedioxynitazene, ethyleneoxynitazene, N-pyrrolidino etonitazene, and N-desethyl isotonitazene utilizing human liver microsomes were investigated, and the metabolites were characterized through high-performance liquid chromatography-high-resolution tandem mass spectrometry. Two to eleven metabolites were identified for different nitazene analogs. It was observed that N-dealkylation, hydroxylation, and dehydrogenation were the major metabolic reactions, with other noteworthy metabolic reactions such as reduction, oxidation, and their combinations also identified. Therefore, it is recommended to use N-dealkylation and hydroxylation metabolites as analytical markers for monitoring of the intake of these substances.","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Over 110 Anabolic-Androgenic Steroids, Corticosteroids and/or Their Esters in Horse Hair Using Ultra-High-Performance Liquid Chromatography-High-Resolution Mass Spectrometry and Gas Chromatography-Tandem Mass Spectrometry After Solid-Supported Liquid Extraction. 超高效液相色谱-高分辨率质谱法和气相色谱-串联质谱法检测马毛中110多种合成代谢激素、皮质激素和/或其酯类物质。

IF 2.6 3区医学

Drug Testing and Analysis Pub Date : 2024-12-10 DOI: 10.1002/dta.3837

Yat-Ming So, Wai Him Kwok, Chris C W Ching, Celia O L Wong, Terence S M Wan, Emmie N M Ho

{"title":"Detection of Over 110 Anabolic-Androgenic Steroids, Corticosteroids and/or Their Esters in Horse Hair Using Ultra-High-Performance Liquid Chromatography-High-Resolution Mass Spectrometry and Gas Chromatography-Tandem Mass Spectrometry After Solid-Supported Liquid Extraction.","authors":"Yat-Ming So, Wai Him Kwok, Chris C W Ching, Celia O L Wong, Terence S M Wan, Emmie N M Ho","doi":"10.1002/dta.3837","DOIUrl":"https://doi.org/10.1002/dta.3837","url":null,"abstract":"Anabolic-androgenic steroids (AAS) are banned substances in both human and equine sports. They are often administered intramuscularly to horses in esterified forms for the purpose of extending their duration of action. As such, the detection of intact esters of endogenous steroids in hair is particularly advantageous, as it can provide unequivocal proof of their external origin. Compared with urine and blood matrices, hair in general allows a longer detection window as long as the administered drug can be incorporated into hair and has not degraded. This can be useful in doping control of equine sports, where the timing of sample collection may be critical due to the transient nature of AAS in other biological fluids. This paper describes the detection of AAS and/or their esters, as well as corticosteroids, in horse hair using ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UPLC-HRMS) and gas chromatography-tandem mass spectrometry (GC-MS/MS) after solid-supported liquid extraction (SLE) at basic pH. The developed UPLC-HRMS and GC-MS/MS methods in combination allowed the detection of 117 AAS, corticosteroids and/or their esters with estimated limits of detection down to sub-ppb levels and with good inter-day precision. Method applicability has been demonstrated through the detections of (i) boldenone undecylenate, nandrolone phenylpropionate and trenbolone acetate in three proficiency testing hair samples and (ii) nandrolone as a metabolite in a post-administration hair sample collected from a castrated horse having been administered nandrolone decanoate.","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142798897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on Internal Standards Applicable to the Detection of Recombinant Erythropoietin. 重组红细胞生成素检测内部标准的研究。

IF 2.6 3区医学

Drug Testing and Analysis Pub Date : 2024-12-05 DOI: 10.1002/dta.3836

Chengshuai Niu, Kaifeng Liu, Xinchao Liu, Sen He, Xinmiao Zhou

引用次数: 0

Analytical and Pharmacological Characterization of 1-(Furan-2-Carbonyl)-LSD (1F-LSD) and Comparison With 1-(Thiophene-2-Carbonyl)-LSD (1T-LSD). 1-（呋喃-2-羰基）- lsd （1F-LSD）的分析和药理特性及与1-（噻吩-2-羰基）- lsd （1T-LSD）的比较。

IF 2.6 3区医学

Drug Testing and Analysis Pub Date : 2024-12-03 DOI: 10.1002/dta.3829

Simon D Brandt, Pierce V Kavanagh, Sarah Gare, Simon P Elliott, Alexander Stratford, Adam L Halberstadt

{"title":"Analytical and Pharmacological Characterization of 1-(Furan-2-Carbonyl)-LSD (1F-LSD) and Comparison With 1-(Thiophene-2-Carbonyl)-LSD (1T-LSD).","authors":"Simon D Brandt, Pierce V Kavanagh, Sarah Gare, Simon P Elliott, Alexander Stratford, Adam L Halberstadt","doi":"10.1002/dta.3829","DOIUrl":"https://doi.org/10.1002/dta.3829","url":null,"abstract":"The classical psychedelic drug (+)-lysergic acid diethylamide (LSD) continues to attract considerable multidisciplinary interest, and over the last eight decades, many derivatives and analogs of LSD have been synthesized. One site on the ergoline scaffold of LSD that has been frequently modified is the N1-position, with the N1-acylated LSD derivative 1-acetyl-LSD (1A-LSD, ALD-52) being one of the earliest examples. In more recent years, several other alkylcarbonyl- and cycloalkylcarbonyl-substituted LSD derivatives have been evaluated, including several distributed as research chemicals. Although N1-substitution is detrimental for the activity of LSD at the 5-HT2A receptor (the primary site of action of psychedelic drugs), N1-acylated LSD derivatives are rapidly hydrolyzed in vivo and are believed to act as prodrugs for LSD. Recently, 1-(thiophene-2-carbonyl)-LSD (1T-LSD, SYN-L-021) was detected as a new recreational drug, signaling a move towards N1-acyl groups with an aromatic character. The present study was conducted to investigate the analytical profile and pharmacology of 1-(2-furoyl)-lysergic acid diethylamide (1F-LSD, SYN-L-005), a novel analog of 1T-LSD. The binding of 1F-LSD to the 5-HT2A receptor and other monoamine sites was assessed using radioligand binding. Furthermore, the in vivo activities of 1F-LSD and 1T-LSD were assessed in C57BL/6 J mice by comparing their biotransformation to LSD and effects on the head-twitch response (HTR), a 5-HT2A-mediated behavior. Both 1F-LSD and 1T-LSD induced the HTR in mice and were hydrolyzed to LSD after in vivo administration, indicating that both substances exhibit LSD-like properties and may serve as prodrugs for LSD.","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142764850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of External Factors on Recovery and Persistence Parameters of Chemical Weapons-Related Alcohols and Thiols in Concrete Samples. 外部因素对化学武器相关醇和硫醇在混凝土样品中回收和持久参数的影响

IF 2.6 3区医学

Drug Testing and Analysis Pub Date : 2024-12-01 DOI: 10.1002/dta.3833

Tomáš Rozsypal, Jakub Pavlík, Ondřej Kareš, Jiří Štoller

{"title":"Influence of External Factors on Recovery and Persistence Parameters of Chemical Weapons-Related Alcohols and Thiols in Concrete Samples.","authors":"Tomáš Rozsypal, Jakub Pavlík, Ondřej Kareš, Jiří Štoller","doi":"10.1002/dta.3833","DOIUrl":"https://doi.org/10.1002/dta.3833","url":null,"abstract":"The contamination of materials in urban areas by chemical weapons is a critical issue, especially as these materials can serve as key evidence in forensic investigations. Concrete, commonly found in urban environments, is highly porous and can retain chemical residues. However, its alkaline nature accelerates the degradation of chemical warfare agents, complicating the recovery of usable evidence. This study explores the recovery and persistence of alcohols and thiols, final degradation products of nerve and blistering agents, from two types of concrete matrices: lightweight concrete formworks and dense, steel-reinforced concrete blocks. Using an optimized method, uncrushed concrete fragments (up to 85 g) were extracted with acetone, monitoring two critical parameters: apparent recovery and persistence. The influence of external conditions, such as water addition, temperatures between 5°C and 35°C, and varying airflow speeds (1.7-5.1 m·s-1), was systematically evaluated. Reference conditions involved dried concrete at 22°C with no airflow. The findings revealed that alcohol recovery aligned with the volatility of the compounds, with denser concrete exhibiting lower recoveries but greater persistence. Thiols quickly converted to disulfides. Notably, temperature and moisture had the most profound effects on the recovery and persistence of the chemicals. These results highlight the importance of considering environmental factors when assessing chemical warfare agents and their degradation products in concrete, offering insights relevant to forensic science, environmental safety, and military defense. The study demonstrates how concrete's properties and external conditions can alter the forensic traceability of chemical contaminants.","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142764911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Doping Control Analysis of Total Carbon Dioxide (TCO₂) in Equine Plasma by Headspace Gas Chromatography-Mass Spectrometry (HS-GC/MS). 利用顶空气相色谱-质谱法 (HS-GC/MS) 对马血浆中的总二氧化碳 (TCO2) 进行兴奋剂控制分析。

IF 2.6 3区医学

Drug Testing and Analysis Pub Date : 2024-11-25 DOI: 10.1002/dta.3832

Karen Y Kwok, Wai Him Kwok, Terence S M Wan, Lydia Brooks, Marie-Agnes Popot, Murielle Jaubert, Ludovic Bailly-Chouriberry, Brendan T Heffron, Chak Kwen Choo, Juanita Tso, Richard Tso, Bob McKenzie, Naomi Selvadurai, David Batty, Bob Gray, Adam Hudson, Stefania Ragazzoni, Mariani Claudio, Emmie N M Ho

{"title":"Doping Control Analysis of Total Carbon Dioxide (TCO2) in Equine Plasma by Headspace Gas Chromatography-Mass Spectrometry (HS-GC/MS).","authors":"Karen Y Kwok, Wai Him Kwok, Terence S M Wan, Lydia Brooks, Marie-Agnes Popot, Murielle Jaubert, Ludovic Bailly-Chouriberry, Brendan T Heffron, Chak Kwen Choo, Juanita Tso, Richard Tso, Bob McKenzie, Naomi Selvadurai, David Batty, Bob Gray, Adam Hudson, Stefania Ragazzoni, Mariani Claudio, Emmie N M Ho","doi":"10.1002/dta.3832","DOIUrl":"https://doi.org/10.1002/dta.3832","url":null,"abstract":"The use of alkalinising agents prior to racing for manipulating performance in the horse has been identified since the 1990s. To mitigate the risk, an international threshold for available carbon dioxide in equine plasma based on analyses using the Beckman Synchron EL-ISE analyser was adopted in 1994 by the International Federation of Horseracing Authorities (IFHA) and revised from 37 to 36 mM in 2004. In 2009, the technical support for the above instrument was discontinued by its manufacturer. Based on the same measurement principle (i.e., ion selective electrode), the Beckman DxC600 analyser was selected as an alternative and validated against the protocol developed by the Association of Official Racing Chemists (AORC). Recently, the DxC600 analyser is also no longer supported by Beckman. Various alternative methods for measuring total carbon dioxide (TCO2) in plasma have been explored. Among these, a headspace gas chromatography-mass spectrometry (HS-GC/MS) method was first reported by the Analytical Forensic Testing Laboratory (AFTL) in 2017. Methods based on the same measurement principle were later developed by different horseracing laboratories. With the objective of cross-validating the new HS-GC/MS methods and to establish an absolute (rather than instrument-dependent or empirical) threshold, an international research collaboration was initiated among different racing laboratories. This paper describes the results of cross-validation studies conducted in November 2019 and December 2022 using horse administration samples from Canada and France, respectively, the determination of a threshold based on population data, and some technical insights on the HS-GC/MS methods.","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142714985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trends in the Detection of Erythropoietin Receptor Agonists (ERAs) in Anti-Doping: An Analysis of Recent Adverse Analytical Findings (AAFs). 反兴奋剂检测促红细胞生成素受体激动剂（ERAs）的趋势：近期阳性分析结果（AAFs）分析》。

IF 2.6 3区医学

Drug Testing and Analysis Pub Date : 2024-11-25 DOI: 10.1002/dta.3828

Tristan Equey, Julian Broséus, Norbert Baume, Reid Aikin

引用次数: 0

A Glimpse Into the Biological Life of Pablo Picasso: Toxicological Study of an Artist's Thumbnail. 巴勃罗-毕加索的生物生活一瞥：对艺术家拇指甲的毒理学研究。

IF 2.6 3区医学

Drug Testing and Analysis Pub Date : 2024-11-21 DOI: 10.1002/dta.3834

Philippe Charlier, Diana Widmaier-Ruiz-Picasso, Jean Claude Alvarez

引用次数: 0