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IF 2.6 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Drug Testing and Analysis Pub Date : 2024-12-14 DOI:10.1002/dta.3840

HuiFen Hannah Zhang, Jia Ying Hilda Loh, Hooi Yan Moy, Chi Pang Lui

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引用次数: 0

摘要

本研究采用半自动样品制备程序和气相色谱-串联质谱法（GC-MS/MS），开发并验证了毛发中多种药物的检测方法。该方法旨在检测和定量毛发中的多种药物，包括苯丙胺、甲基苯丙胺、3,4-亚甲二氧基苯丙胺、3,4-亚甲二氧基甲基苯丙胺、氯胺酮、诺卡他明、可卡因、苯甲酰基可待因、吗啡、可待因和 6-单乙酰吗啡，采用的综合程序包括去污、粉碎、使用支撑液体萃取（SLE）净化和两步衍生化过程。方法验证包括特异性、线性、检出限 (LOD)、定量限 (LOQ)、精密度、准确度、携带率、稳健性和稳定性。结果表明，除甲基苯丙胺的线性范围为 0.2-5 纳克/毫克外，其他药物的线性范围均为 0.1-5 纳克/毫克。该方法的准确度和精密度均在±20%以内，甲基苯丙胺的最低检出限为 0.2 纳克/毫克，苯丙胺和摇头丸为 0.1 纳克/毫克，其余药物为 0.05 纳克/毫克。甲基苯丙胺的检测限为 0.1 纳克/毫克，苯丙胺和摇头丸为 0.05 纳克/毫克，其他药物为 0.025 纳克/毫克。通过使用能力验证服务提供商提供的毛发样本，将定量检测结果与预期结果进行比较，证明了该方法的准确性。这种检测方法可在一次分析中对多种药物进行有效检测，通过减少周转时间和所需的毛发样本量，大大改进了常规检测实验室的毛发分析工作流程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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An Improved GC-MS/MS Method for a Fast Multidrug Analysis in Hair.

This study presents the development and validation of a drug testing method in hair, employing a semi-automated sample preparation procedure and gas chromatography-tandem mass spectrometry (GC-MS/MS) for simultaneous multidrug analysis in hair. The method aims to detect and quantify multiple drugs in hair, including amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, ketamine, norketamine, cocaine, benzoylecgonine, morphine, codeine and 6-monoacetylmorphine, using a comprehensive procedure involving decontamination, pulverization, clean-up using supported-liquid extraction (SLE) and a two-step derivatization process. The method validation included specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, carryover, robustness and stability. The results indicate a linearity range of 0.1-5 ng/mg for all drugs except methamphetamine, which has a linearity range of 0.2-5 ng/mg. The accuracy and precision of the method are within ±20%, with the LOQ at 0.2 ng/mg for methamphetamine, 0.1 ng/mg for amphetamine and MDMA and 0.05 ng/mg for the rest of the drugs. The LODs were found to be 0.1 ng/mg for methamphetamine, 0.05 ng/mg for amphetamine and MDMA and 0.025 ng/mg for other drugs. This method has demonstrated its accuracy by comparing the quantitative test results with the expected results, using hair samples from the proficiency test service providers. This test method offers efficient testing of multiple drugs in a single analysis, significantly improves the hair analysis workflow in a routine testing laboratory by reducing turnaround time and the amount of hair sample required.

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来源期刊

Drug Testing and Analysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

5.90

自引率

24.10%

发文量

191

审稿时长

2.3 months

期刊介绍： As the incidence of drugs escalates in 21st century living, their detection and analysis have become increasingly important. Sport, the workplace, crime investigation, homeland security, the pharmaceutical industry and the environment are just some of the high profile arenas in which analytical testing has provided an important investigative tool for uncovering the presence of extraneous substances. In addition to the usual publishing fare of primary research articles, case reports and letters, Drug Testing and Analysis offers a unique combination of; ‘How to’ material such as ‘Tutorials’ and ‘Reviews’, Speculative pieces (‘Commentaries’ and ‘Perspectives'', providing a broader scientific and social context to the aspects of analytical testing), ‘Annual banned substance reviews’ (delivering a critical evaluation of the methods used in the characterization of established and newly outlawed compounds). Rather than focus on the application of a single technique, Drug Testing and Analysis employs a unique multidisciplinary approach to the field of controversial compound determination. Papers discussing chromatography, mass spectrometry, immunological approaches, 1D/2D gel electrophoresis, to name just a few select methods, are welcomed where their application is related to any of the six key topics listed below.