Journal of immunological methods最新文献_第8页

Mayaro virus and chikungunya virus IgM and IgG testing in parallel improves diagnosis of acute Mayaro virus infections 同时检测马雅罗病毒和基孔肯雅病毒IgM和IgG可改善急性马雅罗病毒感染的诊断

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-10-28 DOI: 10.1016/j.jim.2025.113939

Maite Sabalza , Julia Klemens , Marleen Janku , Erik Lattwein , Anette Schulz , Oliver Klemens

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Evaluation of anti-nuclear antibody (ANA) patterns and its associations with autoimmune disease biomarkers 评估抗核抗体（ANA）模式及其与自身免疫性疾病生物标志物的关联

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-10-28 DOI: 10.1016/j.jim.2025.113924

Vincent Ricchiuti , Min Kyung Lee , David Alfego , Stanley J. Naides , Ajay Grover

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Celiac disease risk stratification: correlation of HLA genotype and celiac-specific antibody positivity 乳糜泻风险分层：HLA基因型与乳糜泻特异性抗体阳性的相关性

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-10-28 DOI: 10.1016/j.jim.2025.113949

Tracie Profaizer , Ann Pole , Michael J. Thompson , Eszter Lazar-Molnar

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Performance characteristics of a vascular endothelial growth factor-D (VEGF-D) ELISA for diagnostic and therapeutic monitoring 用于诊断和治疗监测的血管内皮生长因子- d (VEGF-D) ELISA的性能特征

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-10-28 DOI: 10.1016/j.jim.2025.113964

Lorraine Abushanab, Maria Meriwether, Yongbao Wang, Claire Coeshott

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Improved accessibility of the high precision T-SPOT®.TB test for laboratories and patients through the automation of cell isolation 提高了高精度T-SPOT®的可及性。结核病检测可通过实验室和患者的细胞分离自动化进行

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-10-28 DOI: 10.1016/j.jim.2025.113934

Thomas Jackson-Soutter , Shu-Hua Wang , Andre Trollip , Ruth Brignall , Maite Sabalza , Mathew Quinn , Blanca Restrepo , Mathew Dudek

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Single-tiered serological diagnostic test for Lyme disease 莱姆病单层血清学诊断试验

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-10-28 DOI: 10.1016/j.jim.2025.113941

Kirsten Fagan , Marta Urban , Paul Rocco LaSala , Charles Caso , James Needham , Anu Singh Maharjan

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Establishing biological variation of immunological and inflammatory biomarkers in healthy individuals: a longitudinal study 建立健康个体免疫和炎症生物标志物的生物学变异：一项纵向研究

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-10-28 DOI: 10.1016/j.jim.2025.113947

Eliska Spalkova , Jane Birtwistle , Gabriella Lakos , Selina Howes , Robert van Heezik , Gemma Malin , Isabella Dutton , Satvir Shoker , Khalid Choudhary , Azar Jadir , Paul Moss , Stephen Harding

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Immunogenicity, biodistribution, and toxicology evaluation of Mycobacterium tuberculosis ag85a plasmid DNA in cynomolgus monkeys, mice and guinea pigs 结核分枝杆菌ag85a质粒DNA在食蟹猴、小鼠和豚鼠中的免疫原性、生物分布和毒理学评价

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-08-28 DOI: 10.1016/j.jim.2025.113969

Danyang Zhang , Yourong Yang , Zhen Zhang , Junxian Zhang , Xueqiong Wu , Yan Liang

{"title":"Immunogenicity, biodistribution, and toxicology evaluation of Mycobacterium tuberculosis ag85a plasmid DNA in cynomolgus monkeys, mice and guinea pigs","authors":"Danyang Zhang , Yourong Yang , Zhen Zhang , Junxian Zhang , Xueqiong Wu , Yan Liang","doi":"10.1016/j.jim.2025.113969","DOIUrl":"10.1016/j.jim.2025.113969","url":null,"abstract":"<div><h3>Background</h3><div><em>Mycobacterium tuberculosis</em> (MTB) Ag85A has become a component of multiple new tuberculosis vaccines. It is necessary to evaluate the immunogenicity, biological distribution, and safety of <em>ag85a</em> plasmid DNA (pDNA) to lay the foundation for the design of new vaccines.</div></div><div><h3>Method</h3><div>Chronic toxicity test: cynomolgus monkeys were injected intramuscularly with different doses of <em>ag85a</em> pDNA, and the vaccine absorption kinetics, tissue distribution, and toxicity were observed. Their immune function was evaluated. Acute toxicity test: Mice were injected intramuscularly 0.5 ml saline, and injected intramuscularly and intravenously 0.5 mg/0.5 ml <em>ag85a</em> pDNAs, respectively. The toxicity and death of the mice were observed continuously for 14 days. Allergic test: Guinea pigs were intraperitoneally injected with different doses of <em>ag85a</em> pDNA. After stimulation, the allergic reaction and its severity were observed.</div></div><div><h3>Results</h3><div>Chronic and acute toxicity tests demonstrated that <em>ag85a</em> pDNA injections caused no clinical symptoms or tissue damage. Repeated intramuscular injections in cynomolgus monkeys enhanced specific Th1 immune responses, with pDNA rapidly entering the bloodstream and its concentration positively correlating with dosage. After 8 weeks, <em>ag85a</em> gene was detected only in muscles, myocardium, iliac lymph nodes, and blood. Guinea pig allergy tests showed no weight changes or allergic reactions, even after multiple sensitizations.</div></div><div><h3>Conclusions</h3><div>The <em>ag85a</em> pDNA showed good safety in cynomolgus monkeys, mice, and guinea pigs, and induced high levels of antibodies and T-cell responses, making it a candidate antigen for the construction of a new tuberculosis vaccine.</div></div>","PeriodicalId":16000,"journal":{"name":"Journal of immunological methods","volume":"544 ","pages":"Article 113969"},"PeriodicalIF":1.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CAR binding efficacy and off-target interactions for common CAR detection reagents 常用CAR检测试剂的CAR结合效果及脱靶相互作用

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-08-28 DOI: 10.1016/j.jim.2025.113970

Ryan Sinapius, Jeremy Fisher, Christopher Manning, Amrik Singh

{"title":"CAR binding efficacy and off-target interactions for common CAR detection reagents","authors":"Ryan Sinapius, Jeremy Fisher, Christopher Manning, Amrik Singh","doi":"10.1016/j.jim.2025.113970","DOIUrl":"10.1016/j.jim.2025.113970","url":null,"abstract":"<div><h3>Background</h3><div>Chimeric Antigen Receptor (CAR)-T cell therapy is a highly innovative form of cell-based immunotherapy. To expand CAR-T therapies into additional disease indications, identification of novel tumor antigens and grafting of CARs on other types of immune cells, such as macrophages and natural killer (NK) cells are being pursued. Therefore, as this treatment modality continues to evolve, there is a need for highly specific detection reagents to interrogate CAR surface expression.</div></div><div><h3>Methods/Objectives</h3><div>This study uses flow cytometry to compare the ability of various commercially available CAR detection reagents' to detect CAR constructs containing distinct hinges, linker sequences, and scFv designs. Furthermore, interrogation off-target interactions were performed within a PBMC matrix.</div></div><div><h3>Results</h3><div>All antibody-based CAR detection reagents demonstrated specificity and robust signal in stable CAR-Jurkat cell lines and primary human CAR-T cells. Protein L only detected anti-CD20 (Leu16) scFv-based CAR; off-target non-CAR staining was observed in non-transduced human PBMCs. Detection using recombinant protein antigen provides clean results in single-stain samples for CD19 and BMCA proteins, though CD20 did not produce positive staining in anti-CD20 CARs. Additionally, in live human PBMCs, false-positive signals may be observed for antibody conjugates due to the binding of human Fc receptors to IgG.</div></div><div><h3>Conclusions</h3><div>CAR idiotype antibodies, recombinant antigens, and anti-linker antibodies were able to provide specific and robust detection of CAR-expressing cells. However, the utility of both idiotype antibodies and recombinant antigens is limited to CARs targeting specific antigens. The anti-linker antibodies enable universal and broad detection of CARs independent of target antigens.</div></div>","PeriodicalId":16000,"journal":{"name":"Journal of immunological methods","volume":"544 ","pages":"Article 113970"},"PeriodicalIF":1.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144932472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and evaluation of i-Tracker risankizumab and i-Tracker anti-risankizumab kits: fast and innovative chemiluminescent assays for the monitoring of patients treated with Risankizumab i-Tracker risankizumab和i-Tracker anti-risankizumab试剂盒的开发和评估：快速和创新的化学发光检测，用于监测接受risankizumab治疗的患者

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-10-28 DOI: 10.1016/j.jim.2025.113940

C. Montaillier , S. Daviere , V. Guilbert , G. Noguier , Maite Sabalza , T. Stoor , G. Khater

引用次数: 0