Journal of immunological methods最新文献_第7页

Machine learning can identify an antinuclear antibody pattern that may rule out systemic autoimmune rheumatic diseases 机器学习可以识别抗核抗体模式，可能排除系统性自身免疫性风湿病

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-10-28 DOI: 10.1016/j.jim.2025.113967

May Y. Choi

引用次数: 0

A high-throughput multi-species platform using Biolayer Interferometry Immunosorbent Assay (BLI-ISA) as an alternative to indirect ELISA for vaccine development 使用生物层干涉免疫吸附测定（BLI-ISA）作为间接ELISA替代疫苗开发的高通量多物种平台。

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-09-18 DOI: 10.1016/j.jim.2025.113980

Ti Lu, Md Shafiullah Parvej, Sean K. Whittier, Suhrid Maiti, Zackary K. Dietz, Debaki R. Howlader, Mst Nusrat Zahan, Satabdi Biswas, William D. Picking, Wendy L. Picking

{"title":"A high-throughput multi-species platform using Biolayer Interferometry Immunosorbent Assay (BLI-ISA) as an alternative to indirect ELISA for vaccine development","authors":"Ti Lu, Md Shafiullah Parvej, Sean K. Whittier, Suhrid Maiti, Zackary K. Dietz, Debaki R. Howlader, Mst Nusrat Zahan, Satabdi Biswas, William D. Picking, Wendy L. Picking","doi":"10.1016/j.jim.2025.113980","DOIUrl":"10.1016/j.jim.2025.113980","url":null,"abstract":"<div><div>In vaccine development, the ELISA (Enzyme-Linked Immunosorbent Assay) is commonly used to compare the antibody titers of samples from several treatment groups. This often requires extensive sample preparation, manual labor, and long incubation and processing times. Biolayer Interferometry (BLI) has emerged as an alternative to the ELISA for the detection and quantification of antigen-specific antibodies in biological samples. However, the implementation of BLI as a replacement for the ELISA in vaccine development requires that experimental parameters are established for accurate and reproducible results. Here we give a general protocol for a biolayer interferometry immunosorbent assay (BLI-ISA) for the comparison of antigen-specific antibody levels in treatment group sera that uses secondary antibody binding responses as replacement for ELISA endpoint titers. BLI-ISA provides rapid relative measurements of antigen-specific antibody levels (in nm binding shift), yielding results consistent with ELISA endpoint titers (expressed in ELISA Units), thereby enabling reliable comparison across treatment groups within a fraction of the time required for conventional ELISA.</div></div>","PeriodicalId":16000,"journal":{"name":"Journal of immunological methods","volume":"544 ","pages":"Article 113980"},"PeriodicalIF":1.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Challenges limiting the establishment of standardized clinical cutpoints for antibody tests used in the evaluation of hypersensitivity pneumonitis 限制建立用于评估过敏性肺炎的抗体测试的标准化临床切入点的挑战

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-10-28 DOI: 10.1016/j.jim.2025.113954

Bethany Schuder, Anne Tebo

引用次数: 0

Determining reference values for the ALZPath plasma p-tau217 assay in a large cohort of elderly individuals with normal cognitive function 确定ALZPath血浆p-tau217测定在一大群认知功能正常的老年人中的参考值

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-10-28 DOI: 10.1016/j.jim.2025.113930

Pankaj Kumar , Anna Mammel , Ali Mousavi , Mary Encarnacion , Donald Biehl , Hans Frykman

引用次数: 0

Temperature stability of rabies neutralizing antibodies 狂犬病中和抗体的温度稳定性。

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-10-08 DOI: 10.1016/j.jim.2025.113982

Nicholas J. Jones, Kim A. Appler, Jodie A. Jarvis, Alexander C. Keyel, April D. Davis

引用次数: 0

Fluorescence immunochromatographic assay based on CdSe/ZnS quantum dots for the detection of OXA-48 CdSe/ZnS量子点荧光免疫层析法检测OXA-48。

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-10-02 DOI: 10.1016/j.jim.2025.113984

Mengying Lun , Yumei Chen , Hongliang Liu , Yanhua Qi , Aiping Wang , Jingming Zhou

{"title":"Fluorescence immunochromatographic assay based on CdSe/ZnS quantum dots for the detection of OXA-48","authors":"Mengying Lun , Yumei Chen , Hongliang Liu , Yanhua Qi , Aiping Wang , Jingming Zhou","doi":"10.1016/j.jim.2025.113984","DOIUrl":"10.1016/j.jim.2025.113984","url":null,"abstract":"<div><div>The production of carbapenemase is the primary mechanism of bacterial resistance to carbapenem antibiotics. This resistance seriously compromises the efficacy of carbapenem antibiotics in treating infections and poses a significant challenge to clinical anti-infective therapy. Oxacillinase-48 (OXA-48) is a class D carbapenemase, whose genes are usually located on transferable elements and can spread between different strains and genera. Therefore, from the perspective of infection control, effective monitoring and prevention of OXA-48 are imperative. In this study, CdSe/ZnS quantum dots (QDs) were coupled with an anti-OXA-48 monoclonal antibody (mAb) as a fluorescent signal probe to establish a quantum dot-based fluorescent immunochromatographic strip. The test strip contains a Test line (T-line) coated with an anti-OXA-48 monoclonal antibody and a Control line (C-line) coated with Staphylococcus protein A (SPA). Based on a double-antibody sandwich detection mode, it can complete highly sensitive detection of OXA-48 within 10 min. The visual detection limit of this test strip for OXA-48 recombinant protein was 3.13 ng/mL, and there was no cross-reaction with other common carbapenemases (IMP-1, NDM-1, KPC-2, VIM-2, OXA-23). It can be positioned as a rapid and reliable OXA-48 detection tool, providing a novel method for the rapid identification of OXA-48-producing resistant bacterial strains in clinical practice.</div></div>","PeriodicalId":16000,"journal":{"name":"Journal of immunological methods","volume":"544 ","pages":"Article 113984"},"PeriodicalIF":1.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145228254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Controlling background effects in anti-ganglioside antibody testing 抗神经节苷脂抗体检测中的控制背景效应

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-10-28 DOI: 10.1016/j.jim.2025.113936

Renato Cotti , Jen Stuart , Brandon Beloin , Dominique Keller , Christian-Benedikt Gerhold , Christina Bauer

引用次数: 0

Evaluation of the i-Tracker infliximab and i-Tracker anti-infliximab total antibodies ECLIA on the random-access automated analyzer IDS-iSYS for the routine clinical monitoring of infliximab-treated patients 在IDS-iSYS随机存取自动分析仪上评价i-Tracker英夫利昔单抗和i-Tracker抗英夫利昔单抗总抗体ECLIA对英夫利昔单抗治疗患者的常规临床监测

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-10-28 DOI: 10.1016/j.jim.2025.113933

Akpedje S. Dossou, Serena Kang, Tahira Kalhoro, Eduardo Castro Echeverry, Nathan C. Horton

引用次数: 0

Persistence of criteria antiphospholipid antibodies in relation to titers, isotype and antiphospholipid syndrome diagnosis 标准抗磷脂抗体的持久性与滴度、同型和抗磷脂综合征诊断的关系

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-10-28 DOI: 10.1016/j.jim.2025.113932

Arevik Ghazaryan , Mouhamed Yazan Abou-Ismail , Abdulrahman Saadalla

引用次数: 0

Isolation of HPRT gene depleted human TK6 lymphoblastoid cell line through gamma irradiation γ辐照分离HPRT基因缺失的人TK6淋巴母细胞系。

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-10-10 DOI: 10.1016/j.jim.2025.113985

P.R. Vivek Kumar , Anila Gopinathan , Deepak Sharma

{"title":"Isolation of HPRT gene depleted human TK6 lymphoblastoid cell line through gamma irradiation","authors":"P.R. Vivek Kumar , Anila Gopinathan , Deepak Sharma","doi":"10.1016/j.jim.2025.113985","DOIUrl":"10.1016/j.jim.2025.113985","url":null,"abstract":"<div><div>Ionizing radiation induced DNA DSBs can cause large deletions in the human genome. This property may be utilized for gene disruption to create knockout mutants. The X-linked hypoxanthine–guanine phosphoribosyl transferase (<em>HPRT</em>) gene is commonly used as a reporter to evaluate the frequency and molecular nature of somatic mutations in the human population. Mutations at the <em>HPRT</em> locus are measured through a T lymphocyte cloning assay. This assay relies on lymphoblast feeder cells that support T lymphocyte proliferation via cell-to-cell contact and by helping in the expression of functional interleukin-2 receptors. However, the feeder cells must be <em>HPRT</em>-depleted to prevent feeder cell DNA from serving as substrate during molecular analysis of T lymphocyte mutations. To the best of our knowledge, the human lymphoblastoid TK6 cell line available in Indian cell repositories has an intact <em>HPRT</em> gene (<em>HPRT</em>+). In this study, the parent TK6 cell line was subjected to the clastogenic effects of a high dose gamma radiation to isolate TK6 cells with a total deletion of the <em>HPRT</em> locus. The <em>HPRT</em>-depleted cell line (TK6<sub>LLrrL</sub>) was characterized by mPCR for the loss of exons and the extent of the deletion by flanking STS markers analysis. Further, the feeder cell function of TK6<sub>LLrrL</sub> was evaluated by estimating the cloning efficiency of human peripheral blood lymphocytes. Thus, the study demonstrates the potential of low LET gamma radiation to generate gene knockout cell line. The method described here can be applied to various cell types to produce functionally null cell lines for gene-specific studies.</div></div>","PeriodicalId":16000,"journal":{"name":"Journal of immunological methods","volume":"544 ","pages":"Article 113985"},"PeriodicalIF":1.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145280376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0