Journal of immunological methods最新文献_第6页

Validation of at-home blood sampling for large scale, cost effective serological analysis for anti-viral antibody responses 验证家庭血液采样大规模，成本有效的血清学分析抗病毒抗体反应。

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-12-01 Epub Date: 2025-11-20 DOI: 10.1016/j.jim.2025.114007

Stephen C. Mayer , Jennifer K. Stevenson , Siva Gandhapudi , Jerold G. Woodward

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Impact of oral Bacillus Calmette–Guérin on B220 receptor expression in Peyer's patches of lactating mice: Analysis of receptor percentiles and cell frequencies 口服卡介苗-谷氨酰胺芽孢杆菌对泌乳小鼠Peyer斑中B220受体表达的影响：受体百分位数和细胞频率分析。

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-12-01 Epub Date: 2025-11-05 DOI: 10.1016/j.jim.2025.113995

Ivana Vieira de Mello Loureiro, Claudia Andrea Alves de Araujo

{"title":"Impact of oral Bacillus Calmette–Guérin on B220 receptor expression in Peyer's patches of lactating mice: Analysis of receptor percentiles and cell frequencies","authors":"Ivana Vieira de Mello Loureiro, Claudia Andrea Alves de Araujo","doi":"10.1016/j.jim.2025.113995","DOIUrl":"10.1016/j.jim.2025.113995","url":null,"abstract":"<div><div>The oral administration of the <em>Bacillus Calmette–Guérin</em> (BCG) vaccine aims to replicate the earliest inoculation route. We investigated the impact of this method by analyzing major Peyer's Patches (PP) cell lineages in mice. Oral BCG altered T/CD3, B/B220, double-labeled CD3–B220, double-labeled IgM<img>B220, and triple-labeled CD49b–CD3–B220 cells. Both immunized (IL) and non-immunized (NIL) lactating mice served as controls in the flow cytometry analyses. The findings suggest that oral BCG vaccination induces changes in specific cell lineages, which may have implications for the development of novel vaccination strategies. Since population percentages vary according to source, frequencies based on fluorescence intensity were calculated according to percentiles, and subpopulations were subsequently analyzed per percentile. A robust statistical analysis assessed whether immunization influenced either the percentage of B220-labeled cells or the cellular expression of B220 per bin. The Thy1.2–depleted population revealed B220 as a receptor shared by both B and T lymphocytes. Statistical evaluation by receptor type and cell difference distance revealed subpopulations in transitional phases and showed that, among polychromatic receptor cells such as B220–IgM (CD11b<sup>−</sup>), oral BCG selectively increased B220 expression. Oral BCG also induced CD3 cell activation, evidenced by a decrease in CD5 within the double-labeled CD3–CD5 cluster, despite an overall increase in CD3 receptors. Receptor analysis in regularly turning-over populations, such as PP lymphoid tissue, highlighted the double-labeled B220–CD3 cells. Notably, oral BCG influenced the development of double-labeled B220–CD3 cells in NKT-like populations.</div></div>","PeriodicalId":16000,"journal":{"name":"Journal of immunological methods","volume":"545 ","pages":"Article 113995"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145471061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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An index for deviation distance among amino acid sequences of antibody complementarity determining regions 抗体互补决定区氨基酸序列间的偏离距离指标。

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-12-01 Epub Date: 2025-11-19 DOI: 10.1016/j.jim.2025.114005

Yikeshan Yalikun, Xinyue Qiao, Tyuji Hoshino

{"title":"An index for deviation distance among amino acid sequences of antibody complementarity determining regions","authors":"Yikeshan Yalikun, Xinyue Qiao, Tyuji Hoshino","doi":"10.1016/j.jim.2025.114005","DOIUrl":"10.1016/j.jim.2025.114005","url":null,"abstract":"<div><div>Monoclonal antibodies are currently essential biological molecules for immunotherapy and diagnosis. The adaptability of an antibody to humans is crucial for ensuring safety and achieving high therapeutic efficacy as a medicinal drug. A considerable amount of effort has been devoted to predicting the human likeness of recombinant antibody molecules to assess their suitability for clinical use. Many previous studies have utilized a database of antibody sequences. However, a rapid assessment of adaptability is also helpful in the early stage of high-affinity antibody molecular design to a target antigen. To characterize the amino acid sequences of human antibodies, we statistically analyzed 742 antigen-antibody complex structures extracted from the Protein Data Bank. The frequency and position of the appearances of the respective residues are surveyed, and their probabilities were obtained for three complementarity-determining regions of heavy and light chains. In particular, the populations were examined from the viewpoint of which positions the respective residues were likely to appear in each complementary determining region. Based on a statistical analysis, an arithmetic index was proposed to evaluate sequence compatibility with humans and assess antibody models in computational molecular design. An examination using a collection of neutralizing antibodies for viral infectious diseases suggested that the index can distinguish human antibodies from those of mice. An examination of a collection of approved antibody drugs showed a certain degree of correlation between the calculated index and the immunogenicity of the antibody drugs. These evaluations demonstrated the feasibility of the proposed index as a rapid method for evaluating molecular adaptability to medicinal antibodies.</div></div>","PeriodicalId":16000,"journal":{"name":"Journal of immunological methods","volume":"545 ","pages":"Article 114005"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145573687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A high-throughput assay to measure antibodies that block adhesion of Plasmodium falciparum infected erythrocytes to chondroitin sulfate A. 测定阻断恶性疟原虫感染红细胞对硫酸软骨素A粘附的抗体的高通量测定。

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-12-01 Epub Date: 2025-11-13 DOI: 10.1016/j.jim.2025.114003

Yvonne Dube , Wina Hasang , Andrew Teo , Mwayiwawo Madanitsa , Morten A. Nielsen , Feiko O. Ter Kuile , Elizabeth H. Aitken , Stephen J. Rogerson

{"title":"A high-throughput assay to measure antibodies that block adhesion of Plasmodium falciparum infected erythrocytes to chondroitin sulfate A.","authors":"Yvonne Dube , Wina Hasang , Andrew Teo , Mwayiwawo Madanitsa , Morten A. Nielsen , Feiko O. Ter Kuile , Elizabeth H. Aitken , Stephen J. Rogerson","doi":"10.1016/j.jim.2025.114003","DOIUrl":"10.1016/j.jim.2025.114003","url":null,"abstract":"<div><div>Placental malaria due to <em>Plasmodium falciparum</em> (<em>Pf</em>) is associated with adverse pregnancy outcomes. Infected erythrocytes (IEs) bind to placental chondroitin sulfate A (CSA) and antibodies that inhibit this adhesion are a potential correlate of protection. We developed a simplified adhesion inhibition assay that uses diaminofluorene to measure haemoglobin release from IEs bound to CSA.</div><div>Using hyperimmune plasma, the assay demonstrated concentration-dependent inhibition of CSA adhesion. Assay performance was consistent over time with strong reproducibility (<em>r</em> = 0.82), and results correlated with a published assay (<em>r</em> = 0.53). In 466 Malawian pregnant women (321 <em>P. falciparum</em>-infected and 145 uninfected at first antenatal visit), adhesion inhibitory antibodies were significantly higher in mid-pregnancy in infected multigravidae (46.3 % IQR 23.2 %, 74.8 %) compared to infected primigravidae (9.7 % IQR 0 %, 29.3 %, <em>p</em> < 0.001) and in uninfected multigravidae (29.9 % IQR 8.6 %, 54.5 %) than uninfected primigravidae (15.6 % IQR 0 %, 36.4 %, <em>p</em> = 0.04). Similar, significant gravidity-dependent differences were observed at delivery in both infected and uninfected women. Between enrolment and delivery, changes in antibodies were similar in infected and uninfected women.</div><div>Adhesion inhibitory antibodies protected against placental malaria. Of 162 women with infection at mid-pregnancy, 88 with no placental malaria had more adhesion inhibitory antibody (33.8 %, IQR 1.9 %, 63.2 %) than 74 with past placental malaria (12.5 %, IQR 0 %, 37.8 %; <em>p</em> = 0.002). This low cost, reproducible, and rapid high-throughput adhesion inhibition assay shows promise as a correlate of protection against placental malaria.</div></div>","PeriodicalId":16000,"journal":{"name":"Journal of immunological methods","volume":"545 ","pages":"Article 114003"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145530547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunogenicity of tislelizumab: clinical summary of ADA incidence and feasibility of assessing the suitability of validation cut points using statistical methods tislelizumab的免疫原性：ADA发病率的临床总结和使用统计方法评估验证切入点适用性的可行性。

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-10-02 DOI: 10.1016/j.jim.2025.113983

Boxiang Tao , Meng Wang , Yanfei Yang , Na Zhao , Meiling Tan , Xiaolei Xun , Chunxiang Zeng , Fan Wang

{"title":"Immunogenicity of tislelizumab: clinical summary of ADA incidence and feasibility of assessing the suitability of validation cut points using statistical methods","authors":"Boxiang Tao , Meng Wang , Yanfei Yang , Na Zhao , Meiling Tan , Xiaolei Xun , Chunxiang Zeng , Fan Wang","doi":"10.1016/j.jim.2025.113983","DOIUrl":"10.1016/j.jim.2025.113983","url":null,"abstract":"<div><div>Anti-drug antibody (ADA) is a critical indicator of the immunogenicity of biotherapeutics. To investigate the immunogenicity of tislelizumab, a humanized anti-PD-1 (programmed cell death 1) antibody, 3815 patients were analyzed for ADA incidence. The incidence of ADA is reported for the first time as 19.2 %. For any immunogenicity study, appropriate cut points are essential to accurately distinguish true ADA-positive samples from varying backgrounds across populations. Apart from the standardized false positive rate (FPR) method, statistical methods may be more efficient and powerful in evaluating the appropriateness of cut points. However, a detailed protocol for this approach is not yet in consensus. Therefore, by utilizing extensive data from tislelizumab clinical trials, this study explored the feasibility of assessing the suitability of validation cut points (VCPs) using statistical methods. ADA response datasets from 21 clinical tumor populations were evaluated for distribution differences in comparison to the validation population using statistical methods. Fourteen datasets exhibited significant differences from the validation dataset in both medians and variances, with FPRs exceeding the 2–11 % range. Seven datasets only differed significantly in medians, with five having out-of-range FPRs and their positive rates narrowly affected by cut point alteration. The results showed that statistical methods can effectively assess the suitability of VCPs: in-study cut points are recommended for datasets with significantly different median and variance, while VCPs may be appropriate when only medians differ.</div></div>","PeriodicalId":16000,"journal":{"name":"Journal of immunological methods","volume":"544 ","pages":"Article 113983"},"PeriodicalIF":1.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145228251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Evolution and characterization of β-Lactam allergy profiles and stability of sIgE over two decades in adult patients 20年来成人患者β-内酰胺过敏谱的演变和特征以及sIgE的稳定性

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-10-28 DOI: 10.1016/j.jim.2025.113959

Jessica Murphy , Jose-Julio Laguna , Merce Tena , Cosmin Boteanu , Maria-Luisa Sanchez-Millan

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Donor selection for basophil activation testing 嗜碱性粒细胞激活试验的供体选择

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-10-28 DOI: 10.1016/j.jim.2025.113944

Renee K. Johnson, Amir A. Sadighi Akha, Attila Kumánovics

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Going green: boosting efficiency and sustainability with digital solutions 走向绿色：利用数字解决方案提高效率和可持续性

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-10-28 DOI: 10.1016/j.jim.2025.113958

Jessica Murphy, Phyllis O'Connor

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Validation of a live cell based assay for the detection of MOG antibodies by flow cytometry 流式细胞术检测MOG抗体的活细胞检测验证

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-10-28 DOI: 10.1016/j.jim.2025.113938

Conlan Tran

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Comparative evaluation of pretreatment techniques for enhancing specificity in solid-phase bead-based immunoassays 增强固相珠基免疫测定特异性的预处理技术的比较评价

IF 1.6 4区医学

Journal of immunological methods Pub Date : 2025-11-01 Epub Date: 2025-10-28 DOI: 10.1016/j.jim.2025.113948

Dharmendra Jain , Denise Hurst , Andre Cruz Cruz Delgadillo , Eszter Lázár-Molnár

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