在参加外部质量保证计划监督实验室（EQAPOL）IFN-γ 酶联免疫斑点（IFN-γ ELISpot）检测能力验证计划期间，降低了检测点特定结果的变异性。

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of immunological methods Pub Date : 2024-10-23 DOI:10.1016/j.jim.2024.113770

Paul M. Hargarten , Cassandra G. Porth , Mark Berrong , Darin Weed , Miranda Carper , Thomas N. Denny , Guido Ferrari , Wes Rountree

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引用次数: 0

摘要

NIAID DAIDS 赞助的外部质量保证计划监督实验室 (EQAPOL) 负责管理干扰素-γ (IFN-γ) 酶联免疫斑点 (ELISpot) 外部能力验证计划。ELISpot 计划评估各实验室检测结果的准确性和变异性。该计划中的变异性通过离散度进行量化，离散度是在不同肽池（CMV、CEF）刺激下获得的经背景校正的斑点形成细胞（SFC）复制的方差与平均值之比。本报告包括对2011年至2022年由22个实验室组成的ELISpot计划队列的纵向分析，以评估实验室内的变异性是否随着时间的推移而改善。随机截距模型显示，随着时间的推移，总体离散度明显下降，从2011年的平均约1.8下降到2022年的约1.25。在 21 个观测点中，16 个观测点（4 个具有显著统计学意义）的离散度随时间呈负值趋势。我们发现，实验室内部的总体变异性有所降低，这表明能力验证计划是必要的，也是有益的。

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Decreased variability in the site-specific results during participation in the External Quality Assurance Program Oversight Laboratory (EQAPOL) proficiency program for IFN-gamma enzyme-linked immunospot (IFN-γ ELISpot) assay

The NIAID DAIDS-sponsored External Quality Assurance Program Oversight Laboratory (EQAPOL) manages an interferon-gamma (IFN-γ) enzyme-linked immunospot (ELISpot) external proficiency program. The ELISpot program evaluates the accuracy and variability of results across laboratories. The variability in the program is quantified via the dispersion, which is the ratio of the variance over the mean of the background-corrected spot-forming cells (SFC) replicates obtained under stimulation with different peptide pools (CMV, CEF). This report includes the longitudinal analysis of the ELISpot program cohort composed of 22 laboratories from 2011 to 2022 to assess whether the within-lab variability has improved over time. Random intercept models of the dispersion over time showed a significant decrease in overall dispersion from an average of approximately 1.8 in 2011 to approximately 1.25 in 2022. Out of the 21 sites, 16 sites (4 being statistically significant) had a negative trend for dispersion over time. Our finding of a reduction of overall within-lab variability demonstrates the need for and benefit of proficiency testing programs.

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来源期刊

Journal of immunological methods 医学-免疫学

CiteScore

4.10

自引率

0.00%

发文量

120

审稿时长

3 months

期刊介绍： The Journal of Immunological Methods is devoted to covering techniques for: (1) Quantitating and detecting antibodies and/or antigens. (2) Purifying immunoglobulins, lymphokines and other molecules of the immune system. (3) Isolating antigens and other substances important in immunological processes. (4) Labelling antigens and antibodies. (5) Localizing antigens and/or antibodies in tissues and cells. (6) Detecting, and fractionating immunocompetent cells. (7) Assaying for cellular immunity. (8) Documenting cell-cell interactions. (9) Initiating immunity and unresponsiveness. (10) Transplanting tissues. (11) Studying items closely related to immunity such as complement, reticuloendothelial system and others. (12) Molecular techniques for studying immune cells and their receptors. (13) Imaging of the immune system. (14) Methods for production or their fragments in eukaryotic and prokaryotic cells. In addition the journal will publish articles on novel methods for analysing the organization, structure and expression of genes for immunologically important molecules such as immunoglobulins, T cell receptors and accessory molecules involved in antigen recognition, processing and presentation. Submitted full length manuscripts should describe new methods of broad applicability to immunology and not simply the application of an established method to a particular substance - although papers describing such applications may be considered for publication as a short Technical Note. Review articles will also be published by the Journal of Immunological Methods. In general these manuscripts are by solicitation however anyone interested in submitting a review can contact the Reviews Editor and provide an outline of the proposed review.