Journal of Human Reproductive Sciences最新文献

{"title":"Impact of Previous-day versus Same-day Warming of Cleavage-stage Embryos on Reproductive Outcomes: A Retrospective Cohort Study.","authors":"Swati Singh, Ann Margaret Mangalaraj, Muthukumar Karthikeyan, N Jansi Rani, Aleyamma T Kunjummen, Mohan S Kamath","doi":"10.4103/jhrs.jhrs_98_25","DOIUrl":"10.4103/jhrs.jhrs_98_25","url":null,"abstract":"<p><strong>Background: </strong>Studies have assessed the impact of culturing warmed cleavage-stage embryos to blastocyst in frozen embryo transfer (FET) cycles; there is a paucity of studies that have evaluated the impact of warming of day 3 embryos and culturing for a day before embryo transfer.</p><p><strong>Aim: </strong>The purpose of this research was to study whether warming of day 3 embryos and subsequent transfer on day 4 versus same-day warming and transfer of day 3 embryos improves the treatment outcomes following a FET.</p><p><strong>Settings and design: </strong>We conducted a retrospective cohort study in the Department of Reproductive Medicine and Surgery at Christian Medical College, Vellore, India. All couples who underwent FET at our centre between January 2016 and December 2023 were screened for eligibility.</p><p><strong>Materials and methods: </strong>The patients were divided into two groups according to the stage of the embryo that was transferred: warmed and transferred the same day as a day 3 embryo. The other group in which embryos were warmed the previous day of embryo transfer, cultured for 1 day and were transferred as day 4 embryo. The study groups were matched for age and body mass index by case-control matching. After case-control matching, 209 FET cycles were analysed in either group. The primary outcome was live birth rate (LBR).</p><p><strong>Statistical analysis used: </strong>A parametric <i>t</i>-test or non-parametric Mann-Whitney test was used to find the difference between the groups. The Chi-square and Fisher's exact tests were used to find the association between categorical variables. Univariate and multivariable analysis was performed with a multiple logistic regression model to assess the potential impact of those parameters on LBR.</p><p><strong>Results: </strong>The clinical pregnancy rate per transfer (41.1% vs. 38.2%, <i>P</i> = 0.548) and LBR per transfer (29.1% vs. 27.7%, <i>P</i> = 0.745) were comparable between day 3 versus day 4 transfer groups. Although the cycle cancellation rate was comparable in both the groups (0.8% in day 3 vs. 1.4% in day 4, <i>P</i> = 0.673), cryosurvival of embryos was higher in the day 3 group versus the day 4 group (86.3% vs. 81.7%), and the difference was significant (<i>P</i> < 0.001). Multivariate logistic regression analysis demonstrated that the likelihood of achieving live birth did not depend on day of embryo transfer (odds ratio: 1.059, 95% confidence interval: 0.688-1.628, <i>P</i> = 0.793).</p><p><strong>Conclusion: </strong>Warming cleavage-stage embryos and culturing for 1 extra day did not show any significant difference in FET outcomes.</p>","PeriodicalId":15975,"journal":{"name":"Journal of Human Reproductive Sciences","volume":"18 3","pages":"141-147"},"PeriodicalIF":1.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12527148/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145308293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gonadotropin-releasing Hormone Antagonist versus Fixed Progestin-Primed Ovarian Stimulation in <i>In vitro</i> Fertilisation: A Cohort Study.","authors":"Manjushri Amol Kothekar, Richa A Jagtap, Parul Katiyar, Human M Fatemi, Barbara Lawrenz","doi":"10.4103/jhrs.jhrs_97_25","DOIUrl":"10.4103/jhrs.jhrs_97_25","url":null,"abstract":"<p><strong>Background: </strong>Investigators have compared progestin-primed ovarian stimulation (PPOS) and conventional stimulations in multiple studies, however, with contrasting observations with respect to <i>in vitro</i> fertilisation (IVF) cycle outcomes. The extent of serum luteinising hormone (LH) suppression has not been studied much between the protocols.</p><p><strong>Aim: </strong>The primary aim of this study was to compare IVF cycle outcomes in terms of oocyte yield, maturation rate, fertilisation rate, blastulation rate, implantation and cumulative pregnancy rate between gonadotropin-releasing hormone (GnRH) antagonist protocol and fixed PPOS protocol. The secondary aim was to compare the extent of pituitary suppression as reflected by serum LH before final oocyte maturation between both the protocols.</p><p><strong>Settings and design: </strong>This was a retrospective observational cohort study.</p><p><strong>Materials and methods: </strong>All ovarian stimulation cycles, either performed as GnRH-antagonist or as fixed PPOS, between 1^st October 2023 and 15^th July 2024, at a tertiary level IVF centre were included. Egg freezing cycles and donor egg cycles were excluded.</p><p><strong>Statistical analysis used: </strong>Statistical tests applied were Student's <i>t</i>-test two-tailed for interval/ratio variables and the Chi-square test for nominal variables with a significance level of 0.05.</p><p><strong>Results: </strong>The total number of cycles was 189, of which 87 (46.03%) cycles were in the GnRH antagonist group and 102 (53.96%) in the PPOS group. The groups were similar with respect to age, anti-Müllerian hormone, body mass index as well as total gonadotropin consumption. There was no significant difference observed for the number of oocytes (<i>P</i> = 0.92), maturation rate (<i>P</i> = 0.07), fertilisation rate (<i>P</i> = 0.84), blastulation rate (<i>P</i> = 0.52), implantation rate (<i>P</i> = 0.36) and cumulative pregnancy rate (<i>P</i> = 0.63). The serum LH values before final oocyte maturation (suppressed LH) were similar (<i>P</i> = 0.09) in both the protocols.</p><p><strong>Conclusion: </strong>IVF cycle outcomes with respect to oocyte yield, maturation rate, fertilisation rate, blastulation rate, implantation rates and cumulative pregnancy were comparable between GnRH antagonist and PPOS cycles. Furthermore, the extent of pituitary suppression, reflected by the serum LH levels before final oocyte maturation, was similar between the groups as observed in our analysis. However, these findings need to be validated by prospective studies or randomised trials with equal patient division.</p>","PeriodicalId":15975,"journal":{"name":"Journal of Human Reproductive Sciences","volume":"18 3","pages":"136-140"},"PeriodicalIF":1.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12527153/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145308246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Commentary.","authors":"Mohan S Kamath","doi":"10.4103/jhrs.jhrs_205_25","DOIUrl":"https://doi.org/10.4103/jhrs.jhrs_205_25","url":null,"abstract":"","PeriodicalId":15975,"journal":{"name":"Journal of Human Reproductive Sciences","volume":"18 3","pages":"135"},"PeriodicalIF":1.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12527155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145308287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Angiotensin-converting Enzyme Insertion/Deletion Polymorphism Distribution in the North Indian Population of Jammu and Kashmir and Its Positive Correlation with Male Infertility: A Case Control Study.","authors":"Sonali Bhan, Manisha Bhagat, Shreya Sopori, Arti Dhar, Rakesh Kumar, Audesh Bhat","doi":"10.4103/jhrs.jhrs_79_25","DOIUrl":"10.4103/jhrs.jhrs_79_25","url":null,"abstract":"<p><strong>Background: </strong>A precise balance among angiotensin-converting enzyme (ACE), angiotensin-II (Ang-II) and Ang-II type 1 receptor is essential for spermatogenesis. Any alteration in this balance can potentially lead to infertility.</p><p><strong>Aim: </strong>To study the potential association of the <i>ACE</i> Insertion (I)/deletion (D) polymorphism with male infertility in the North Indian population of Jammu and Kashmir.</p><p><strong>Settings and design: </strong>This case-control genetic association study was conducted on 362 subjects in the North Indian population of Jammu and Kashmir. All participants provided informed consent, and the study was approved by the institutional human ethics committee.</p><p><strong>Materials and methods: </strong>DNA was extracted from blood using a kit (Qiagen Pvt. Ltd., Germany). The primer sequence was taken from the literature. Ready-to-use PCR mix from Takara Bioscience was used to genotype the <i>ACE</i> I/D polymorphism in 162 infertile men and 200 healthy controls.</p><p><strong>Statistical analysis used: </strong>Logistic regression, <i>t</i>-test and Chi-square were used to perform the statistical comparisons between cases and controls using SPSS v25.0 and other online statistical tools.</p><p><strong>Results: </strong>The D allele frequency in combined cases was significantly higher than in the control group, with values of 0.52 compared to 0.34 (<i>P</i> = 0.04). Similarly, the genotypic frequency distribution between cases and controls was significantly different, with cases having a higher prevalence of the DD genotype than controls (27.7% vs. 9.5%, respectively). Our data suggest that men with the DD genotype are approximately five times more likely to experience infertility than those with the II genotype in the study population, with an odds ratio (OR) of 4.94 (95% confidence interval [CI] 2.45-9.97). Furthermore, infertile men with the DD genotype exhibited lower sperm count (54.2 ± 13.1 million/mL) and progressive motility (41.2 ± 12.8%) compared to the II carriers (sperm counts 59.1 ± 13.2 million/mL and progressive motility 47.7 ± 11.5%). When analysed separately, only asthenoteratozoospermic and idiopathic cases showed a highly significant association with the DD genotype when compared with the control group (OR 4.7 [95% CI 2.09-10.34] and OR 6.3 [95% CI 2.79-14.90], respectively).</p><p><strong>Conclusion: </strong>Our study is the first report linking the <i>ACE</i> I/D polymorphism to male infertility in any Indian population. The D allele appears to pose a greater risk for male infertility in the study population, particularly for asthenoteratozoospermic and idiopathic infertility.</p>","PeriodicalId":15975,"journal":{"name":"Journal of Human Reproductive Sciences","volume":"18 3","pages":"166-173"},"PeriodicalIF":1.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12527149/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145308333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Translation, Cross-cultural Adaptation and Validation of Hindi Version of Polycystic Ovary Syndrome-quality of Life Scale (PCOSQOL-I) - A Cross-sectional study.","authors":"Reeta Mahey, Sujata Satapathy, Partha Haldar, Supriya Kumari, Monica Gupta, Aarthi K Jayraj, Archana Kumari, Rohitha Cheluvaraju, Rekha Ramappa, Ayushi Negi","doi":"10.4103/jhrs.jhrs_84_25","DOIUrl":"10.4103/jhrs.jhrs_84_25","url":null,"abstract":"<p><strong>Background: </strong>With specific symptomatology and clinical presentation, the validity of standard quality of life assessment scales is questionable for polycystic ovary syndrome (PCOS) women.</p><p><strong>Aim: </strong>The aim of the study was to translate and validate the Hindi version of the PCOS-related Quality of Life (PCOSQOL) scale into culturally adaptable, screening tool for Indian women with PCOS.</p><p><strong>Settings and design: </strong>A cross-sectional study was conducted in the outpatient department of a tertiary care hospital.</p><p><strong>Materials and methods: </strong>After standard translation and linguistic validation, modifications were done as per culturally acceptable items. After the item content validity index and scale content validity ratio testing, the preliminary tool was prepared. After pilot testing and minor modifications, the final PCOSQOL for Indian Women (PCOSQOL-I) tool was applied to 287 PCOS women to check reliability, exploratory factor analysis, uniqueness, and factor loading.</p><p><strong>Statistical analysis used: </strong>R (version 4.3.0 [2023-04-21]).</p><p><strong>Results: </strong>The CVI of the 27-item Hindi tool was 0.73, 0.83, 0.70, and 0.72, respectively, for relevance, clarity, appropriateness, and necessity, thus indicating recommended content validity. A three-factor solution emerged through exploratory factor analysis with proportional variance for factors 1 (impact of PCOS), 2 (hirsutism), and 3 (infertility) as 0.22, 0.12, and 0.10, respectively. The cumulative variance was 0.22, 0.35, and 0.44, respectively. There was high internal consistency (Cronbach Alpha 0.89). On factor loadings, and dropping items with value <0.3, final 25-item PCOSQOL-I scale was developed where each item scored 0-4 with higher score indicating better quality of life.</p><p><strong>Conclusion: </strong>The PCOSQOL-I scale has good reliability and may be used to screen the quality of life for PCOS women. Further testing of the tool is warranted to check its competency to differentiate among PCOS women with different phenotypes.</p>","PeriodicalId":15975,"journal":{"name":"Journal of Human Reproductive Sciences","volume":"18 2","pages":"96-104"},"PeriodicalIF":1.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12306719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Individualisation of the Dose of Letrozole for Ovulation Induction in Infertile Women with Polycystic Ovary Syndrome Based on Clinical and Ultrasonography Criteria: A Cohort Study.","authors":"Pratyasha Peepal, Padmalaya Thakur, Anubhuti Patel, Jyotiranjan Sahoo, Sujata Pradhan, Manisha Sahu","doi":"10.4103/jhrs.jhrs_72_25","DOIUrl":"10.4103/jhrs.jhrs_72_25","url":null,"abstract":"<p><strong>Background: </strong>Conventionally, letrozole for ovulation induction (OI) in polycystic ovary syndrome (PCOS) is started at a dose of 2.5 mg, which is gradually escalated depending on the follicular response. The minimum dose required for follicular response for a particular patient is yet to be determined.</p><p><strong>Aim: </strong>The aim of the study was to compare various clinical and ultrasonographic characteristics of patients with successful OI with different doses of letrozole and individualise the dose of letrozole.</p><p><strong>Setting and design: </strong>This prospective cohort study was conducted in a tertiary care teaching hospital from December 2019 to March 2024.</p><p><strong>Materials and methods: </strong>Ninety-seven patients were treated with a starting dose of 2.5 mg of letrozole. The dose was increased to 5 and 7.5 mg without a follicular response. Various clinical and ultrasonographic characteristics were compared.</p><p><strong>Statistical analysis used: </strong>Data entered in Microsoft Excel and analysed using SPSS version 27. <i>P</i> < 0.05 was statistically significant.</p><p><strong>Results: </strong>Of 97 patients, 10 were lost to follow-up. Eighty-one (93.1%) patients had a positive response to letrozole. Six (6.9%) patients did not respond to the maximum dose of 7.5 mg of letrozole. Waist circumference, maximum antral follicle count per ovary and volume of the largest ovary were significantly different for patients who responded to different doses of letrozole (<i>P</i> < 0.05). Further analysis revealed that an ovarian volume of 10 cc or more is a risk factor for non-response to 2.5 mg of letrozole.</p><p><strong>Conclusion: </strong>The ovarian volume can be considered when determining the dose of letrozole in PCOS.</p>","PeriodicalId":15975,"journal":{"name":"Journal of Human Reproductive Sciences","volume":"18 2","pages":"89-95"},"PeriodicalIF":1.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12306715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond the Inner Cell Mass: Evaluating the Impact of Inner Cell Mass Visibility on Implantation Prediction in Static Blastocyst Images - A Retrospective Cohort Study.","authors":"Doel Bose Pande, Hemant Deshmukh, Sandeep Kumar Verma, Gajendra Singh Tomar","doi":"10.4103/jhrs.jhrs_63_25","DOIUrl":"10.4103/jhrs.jhrs_63_25","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Embryo selection in IVF often relies on inner cell mass (ICM) morphology as a predictor of implantation. However, in static two-dimensional images, ICM visibility may be limited by technical factors like orientation or focus, rather than actual quality. Since most IVF labs still rely on static images for embryo evaluation, it is important to reconsider the emphasis placed on ICM and investigate other morphological features that may support clinical decision-making.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Aim: &lt;/strong&gt;To assess whether the visibility of the inner cell mass (ICM) in static blastocyst images affects the accuracy of embryologists in predicting clinical pregnancy, defined by the presence of fetal cardiac activity.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Settings and design: &lt;/strong&gt;A retrospective cohort study conducted at a single IVF center, analyzing 300 single vitrified warmed blastocyst transfer cycles between 2021 and 2024. Two senior embryologists independently evaluated static blastocyst images, blinded to clinical metadata. Clinical pregnancy, confirmed by fetal cardiac activity at 7 to 8 weeks, was used as the ground truth for measuring prediction accuracy.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Materials and methods: &lt;/strong&gt;This retrospective study analysed 300 single vitrified warmed blastocyst transfers performed at a single IVF centre between 2021 and 2024. Only Day 5 blastocysts that re-expanded within 2-3 hours post-warming were included. Static images captured just before transfer were evaluated independently by two senior embryologists, who were blinded to clinical outcomes and to each other's assessments. ICM visibility (Good/Poor) and binary implantation predictions were recorded. Clinical pregnancy was determined by the presence of fetal cardiac activity at 7-8 weeks of gestation.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Statistical analysis used: &lt;/strong&gt;Chi square tests were performed to compare prediction accuracy between good and poor ICM visibility groups for overall, positive and negative predictions. A &lt;i&gt;P&lt;/i&gt; value less than 0.05 was considered statistically significant.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;A total of 300 blastocysts were analysed to assess the impact of ICM visibility on the accuracy of implantation prediction by embryologists. While embryos with good ICM visibility comprised 63% of cases (n = 189), prediction accuracy was slightly higher for embryos with poor ICM visibility (44.14%) than for those with good visibility (39.68%), although the difference was not statistically significant. Chi square analysis showed no significant association between ICM visibility and accuracy for either positive (&lt;i&gt;P&lt;/i&gt; = 0.0652) or negative (&lt;i&gt;P&lt;/i&gt; = 0.9220) predictions.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;Our findings suggest that the predictive accuracy of embryo outcomes does not significantly differ whether the ICM is clearly visible or not in the static 2D image. This supports the need to focus on other morphological and morphometric features that can be consistently","PeriodicalId":15975,"journal":{"name":"Journal of Human Reproductive Sciences","volume":"18 2","pages":"80-88"},"PeriodicalIF":1.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12306722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Psychological Distress and Fatigue in Polycystic Ovarian Syndrome: A Pilot Study.","authors":"Aakanksha Bajpai, Digvijay Sharma","doi":"10.4103/jhrs.jhrs_25_25","DOIUrl":"10.4103/jhrs.jhrs_25_25","url":null,"abstract":"<p><strong>Background: </strong>Polycystic ovarian syndrome (PCOS) is a complex endocrine disorder affecting both physical and psychological health in women of reproductive age. While its metabolic and reproductive symptoms are well-documented, fatigue remains an underrecognised yet significant concern. Psychological distress, including anxiety, depression and stress, may contribute to fatigue in women with PCOS, either directly or by influencing sleep disturbances. While insomnia is commonly reported in this population, this study primarily focuses on fatigue and its association with psychological distress, with sleep disturbances considered as a secondary factor requiring further exploration.</p><p><strong>Aim: </strong>This study aims to investigate the association between psychological distress (anxiety, depression and stress) and fatigue in women with PCOS. In addition, it explores whether sleep disturbances show any association with fatigue, providing insights into factors that may contribute to fatigue severity in this population.</p><p><strong>Settings and design: </strong>This is a cross-sectional pilot study conducted in a university outpatient department.</p><p><strong>Materials and methods: </strong>A total of 52 women, diagnosed with PCOS were included in this cross-sectional study. Data collection involves scoring of fatigue by administering the fatigue severity scale (FSS) and insomnia severity index (ISI) for insomnia and components of psychological distress (stress, depression and anxiety) using the Depression Anxiety Stress Scale-21.</p><p><strong>Statistical analysis used: </strong>Data were analysed using IBM SPSS version 25. Pearson correlation coefficients were used to explore bivariate relationships between fatigue and independent variables. Statistical significance was set at <i>P</i> < 0.05.</p><p><strong>Results: </strong>The findings revealed significant associations between FSS and psychological distress, particularly anxiety (<i>r</i> = 0.507, <i>P</i> = 0.038) and depression (<i>r</i> = 0.595, <i>P</i> = 0.012). In addition, insomnia (ISI) showed the strongest correlation with fatigue (<i>r</i> = 0.705, <i>P</i> = 0.002), indicating that sleep disturbances may further exacerbate fatigue in women with PCOS.</p><p><strong>Conclusion: </strong>This study highlights a strong association between psychological distress, insomnia and fatigue in women with PCOS. The findings suggest that higher levels of anxiety, depression and sleep disturbances contribute significantly to fatigue severity, emphasising the need for integrated psychological and sleep management strategies in PCOS care. Further research with larger cohorts and longitudinal designs is necessary to establish causal relationships and develop targeted interventions for improving overall well-being in this population.</p>","PeriodicalId":15975,"journal":{"name":"Journal of Human Reproductive Sciences","volume":"18 2","pages":"113-117"},"PeriodicalIF":1.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12306717/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Severe Early-onset Ovarian Hyperstimulation Syndrome with Isolated Pleural Effusion following Agonist Trigger in Gonadotropin-releasing Hormone Antagonist Cycle: A Case Report.","authors":"Pooja Dhir Bhayana, Priyanka Singh, Shruti Passan","doi":"10.4103/jhrs.jhrs_39_25","DOIUrl":"10.4103/jhrs.jhrs_39_25","url":null,"abstract":"<p><p>The use of gonadotrophin-releasing hormone (GnRH) agonist trigger in GnRH antagonist cycles in <i>in vitro</i> fertilisation (IVF) with elective freeze-all strategy is known to practically eliminate the risk of severe ovarian hyperstimulation syndrome (OHSS). Still, there are few case reports of early-onset severe OHSS development after agonist use for final oocyte maturation. This patient presented in an emergency on post-Ovum pickup (OPU) day 5 with complaints of cough with chest pain. Her ultrasound chest revealed bilateral massive pleural effusion right > left and required pleural tap on day 7 post-OPU. On Ultrasound abdomen, she was found to have B/L enlarged ovaries consistent with post-OPU status with minimal ascites. She was managed conservatively and discharged in stable condition. This case report emphasises that severe OHSS can develop even after agonist trigger with the segmentation of IVF. The best strategy to prevent OHSS is to keep the stimulation to lower side in patients at risk and take all due precautions to prevent the same.</p>","PeriodicalId":15975,"journal":{"name":"Journal of Human Reproductive Sciences","volume":"18 2","pages":"125-128"},"PeriodicalIF":1.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12306716/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Non-invasive Preimplantation Genetic Testing-Aneuploidy Using Spent Culture Media and its Concordance with Trophectoderm Biopsy: A Proof of Concept and Validation Study.","authors":"Neeta Singh, Ankita Sethi, Lata Rani, Monika Saini, Ritu Gupta, Neena Malhotra, Reeta Mahey","doi":"10.4103/jhrs.jhrs_192_24","DOIUrl":"10.4103/jhrs.jhrs_192_24","url":null,"abstract":"<p><strong>Background: </strong>While trophectoderm (TE) biopsy with next-generation sequencing (NGS) remains the gold standard for preimplantation genetic testing for aneuploidy (PGT-A), the discovery of cell-free DNA (cfDNA) in spent culture media (SCM) has sparked interest in non-invasive PGT-A (NiPGT-A) as a potential alternative.</p><p><strong>Aim: </strong>The study was conducted to assess the feasibility of cell cfDNA from SCM as a tool for NiPGT-A in patients undergoing IVF for advanced age, repeated implantation failure or severe male factor infertility.</p><p><strong>Settings and design: </strong>This is a prospective study where a total of 44 embryos having TE biopsy for aneuploidy testing and their respective SCM collected at day 5/6 were analysed.</p><p><strong>Materials and methods: </strong>All aneuploid blastocysts (WB) were subjected to DNA extraction and amplification using Sureplex DNA amplification system followed by library preparation using VeriSeq™ PGS Library Prep kit and sequencing on MiSeq (Illumina, California, USA).</p><p><strong>Statistical analysis used: </strong>Copy Number Variation visualisation and analysis were carried out using BlueFuse Multi Software (Illumina). The statistical data were analysed by STATA version 14.</p><p><strong>Results: </strong>Informative results were obtained in 36/44 (81.2%) SCM samples. The reads were analysable in 26 paired (SCM and TE biopsy) samples. Concordant NGS results for both TE biopsy and SCM sample were obtained in 17/26 (65.38%) embryos. The per chromosome concordance rate was 85.13% (487/572) and the sex chromosome concordance rate was 73% (19/26). The sensitivity and specificity of NiPGT-A were 66.6% and 60%, respectively. On comparing the ploidy concordance rate, poor morphology embryos had better, but not statistically significant concordance rate (83.33%) as compared to good morphology embryos (50%, <i>P</i> = 0.16). Although not significant, day 6 embryos had better per chromosome as well as sex chromosome concordance rate as compared to day 5 embryos.</p><p><strong>Conclusion: </strong>Aneuploidy testing using cf DNA in SCM is a promising technique but needs more research on larger cohort size to improve the sensitivity, specificity and concordance rate.</p>","PeriodicalId":15975,"journal":{"name":"Journal of Human Reproductive Sciences","volume":"18 2","pages":"70-79"},"PeriodicalIF":1.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12306720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}