Journal of Human Reproductive Sciences最新文献

{"title":"Translation, Cross-cultural Adaptation and Validation of Hindi Version of Polycystic Ovary Syndrome-quality of Life Scale (PCOSQOL-I) - A Cross-sectional study.","authors":"Reeta Mahey, Sujata Satapathy, Partha Haldar, Supriya Kumari, Monica Gupta, Aarthi K Jayraj, Archana Kumari, Rohitha Cheluvaraju, Rekha Ramappa, Ayushi Negi","doi":"10.4103/jhrs.jhrs_84_25","DOIUrl":"10.4103/jhrs.jhrs_84_25","url":null,"abstract":"<p><strong>Background: </strong>With specific symptomatology and clinical presentation, the validity of standard quality of life assessment scales is questionable for polycystic ovary syndrome (PCOS) women.</p><p><strong>Aim: </strong>The aim of the study was to translate and validate the Hindi version of the PCOS-related Quality of Life (PCOSQOL) scale into culturally adaptable, screening tool for Indian women with PCOS.</p><p><strong>Settings and design: </strong>A cross-sectional study was conducted in the outpatient department of a tertiary care hospital.</p><p><strong>Materials and methods: </strong>After standard translation and linguistic validation, modifications were done as per culturally acceptable items. After the item content validity index and scale content validity ratio testing, the preliminary tool was prepared. After pilot testing and minor modifications, the final PCOSQOL for Indian Women (PCOSQOL-I) tool was applied to 287 PCOS women to check reliability, exploratory factor analysis, uniqueness, and factor loading.</p><p><strong>Statistical analysis used: </strong>R (version 4.3.0 [2023-04-21]).</p><p><strong>Results: </strong>The CVI of the 27-item Hindi tool was 0.73, 0.83, 0.70, and 0.72, respectively, for relevance, clarity, appropriateness, and necessity, thus indicating recommended content validity. A three-factor solution emerged through exploratory factor analysis with proportional variance for factors 1 (impact of PCOS), 2 (hirsutism), and 3 (infertility) as 0.22, 0.12, and 0.10, respectively. The cumulative variance was 0.22, 0.35, and 0.44, respectively. There was high internal consistency (Cronbach Alpha 0.89). On factor loadings, and dropping items with value <0.3, final 25-item PCOSQOL-I scale was developed where each item scored 0-4 with higher score indicating better quality of life.</p><p><strong>Conclusion: </strong>The PCOSQOL-I scale has good reliability and may be used to screen the quality of life for PCOS women. Further testing of the tool is warranted to check its competency to differentiate among PCOS women with different phenotypes.</p>","PeriodicalId":15975,"journal":{"name":"Journal of Human Reproductive Sciences","volume":"18 2","pages":"96-104"},"PeriodicalIF":1.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12306719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Individualisation of the Dose of Letrozole for Ovulation Induction in Infertile Women with Polycystic Ovary Syndrome Based on Clinical and Ultrasonography Criteria: A Cohort Study.","authors":"Pratyasha Peepal, Padmalaya Thakur, Anubhuti Patel, Jyotiranjan Sahoo, Sujata Pradhan, Manisha Sahu","doi":"10.4103/jhrs.jhrs_72_25","DOIUrl":"10.4103/jhrs.jhrs_72_25","url":null,"abstract":"<p><strong>Background: </strong>Conventionally, letrozole for ovulation induction (OI) in polycystic ovary syndrome (PCOS) is started at a dose of 2.5 mg, which is gradually escalated depending on the follicular response. The minimum dose required for follicular response for a particular patient is yet to be determined.</p><p><strong>Aim: </strong>The aim of the study was to compare various clinical and ultrasonographic characteristics of patients with successful OI with different doses of letrozole and individualise the dose of letrozole.</p><p><strong>Setting and design: </strong>This prospective cohort study was conducted in a tertiary care teaching hospital from December 2019 to March 2024.</p><p><strong>Materials and methods: </strong>Ninety-seven patients were treated with a starting dose of 2.5 mg of letrozole. The dose was increased to 5 and 7.5 mg without a follicular response. Various clinical and ultrasonographic characteristics were compared.</p><p><strong>Statistical analysis used: </strong>Data entered in Microsoft Excel and analysed using SPSS version 27. <i>P</i> < 0.05 was statistically significant.</p><p><strong>Results: </strong>Of 97 patients, 10 were lost to follow-up. Eighty-one (93.1%) patients had a positive response to letrozole. Six (6.9%) patients did not respond to the maximum dose of 7.5 mg of letrozole. Waist circumference, maximum antral follicle count per ovary and volume of the largest ovary were significantly different for patients who responded to different doses of letrozole (<i>P</i> < 0.05). Further analysis revealed that an ovarian volume of 10 cc or more is a risk factor for non-response to 2.5 mg of letrozole.</p><p><strong>Conclusion: </strong>The ovarian volume can be considered when determining the dose of letrozole in PCOS.</p>","PeriodicalId":15975,"journal":{"name":"Journal of Human Reproductive Sciences","volume":"18 2","pages":"89-95"},"PeriodicalIF":1.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12306715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond the Inner Cell Mass: Evaluating the Impact of Inner Cell Mass Visibility on Implantation Prediction in Static Blastocyst Images - A Retrospective Cohort Study.","authors":"Doel Bose Pande, Hemant Deshmukh, Sandeep Kumar Verma, Gajendra Singh Tomar","doi":"10.4103/jhrs.jhrs_63_25","DOIUrl":"10.4103/jhrs.jhrs_63_25","url":null,"abstract":"<p><strong>Background: </strong>Embryo selection in IVF often relies on inner cell mass (ICM) morphology as a predictor of implantation. However, in static two-dimensional images, ICM visibility may be limited by technical factors like orientation or focus, rather than actual quality. Since most IVF labs still rely on static images for embryo evaluation, it is important to reconsider the emphasis placed on ICM and investigate other morphological features that may support clinical decision-making.</p><p><strong>Aim: </strong>To assess whether the visibility of the inner cell mass (ICM) in static blastocyst images affects the accuracy of embryologists in predicting clinical pregnancy, defined by the presence of fetal cardiac activity.</p><p><strong>Settings and design: </strong>A retrospective cohort study conducted at a single IVF center, analyzing 300 single vitrified warmed blastocyst transfer cycles between 2021 and 2024. Two senior embryologists independently evaluated static blastocyst images, blinded to clinical metadata. Clinical pregnancy, confirmed by fetal cardiac activity at 7 to 8 weeks, was used as the ground truth for measuring prediction accuracy.</p><p><strong>Materials and methods: </strong>This retrospective study analysed 300 single vitrified warmed blastocyst transfers performed at a single IVF centre between 2021 and 2024. Only Day 5 blastocysts that re-expanded within 2-3 hours post-warming were included. Static images captured just before transfer were evaluated independently by two senior embryologists, who were blinded to clinical outcomes and to each other's assessments. ICM visibility (Good/Poor) and binary implantation predictions were recorded. Clinical pregnancy was determined by the presence of fetal cardiac activity at 7-8 weeks of gestation.</p><p><strong>Statistical analysis used: </strong>Chi square tests were performed to compare prediction accuracy between good and poor ICM visibility groups for overall, positive and negative predictions. A <i>P</i> value less than 0.05 was considered statistically significant.</p><p><strong>Results: </strong>A total of 300 blastocysts were analysed to assess the impact of ICM visibility on the accuracy of implantation prediction by embryologists. While embryos with good ICM visibility comprised 63% of cases (n = 189), prediction accuracy was slightly higher for embryos with poor ICM visibility (44.14%) than for those with good visibility (39.68%), although the difference was not statistically significant. Chi square analysis showed no significant association between ICM visibility and accuracy for either positive (<i>P</i> = 0.0652) or negative (<i>P</i> = 0.9220) predictions.</p><p><strong>Conclusion: </strong>Our findings suggest that the predictive accuracy of embryo outcomes does not significantly differ whether the ICM is clearly visible or not in the static 2D image. This supports the need to focus on other morphological and morphometric features that can be consistently","PeriodicalId":15975,"journal":{"name":"Journal of Human Reproductive Sciences","volume":"18 2","pages":"80-88"},"PeriodicalIF":1.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12306722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Psychological Distress and Fatigue in Polycystic Ovarian Syndrome: A Pilot Study.","authors":"Aakanksha Bajpai, Digvijay Sharma","doi":"10.4103/jhrs.jhrs_25_25","DOIUrl":"10.4103/jhrs.jhrs_25_25","url":null,"abstract":"<p><strong>Background: </strong>Polycystic ovarian syndrome (PCOS) is a complex endocrine disorder affecting both physical and psychological health in women of reproductive age. While its metabolic and reproductive symptoms are well-documented, fatigue remains an underrecognised yet significant concern. Psychological distress, including anxiety, depression and stress, may contribute to fatigue in women with PCOS, either directly or by influencing sleep disturbances. While insomnia is commonly reported in this population, this study primarily focuses on fatigue and its association with psychological distress, with sleep disturbances considered as a secondary factor requiring further exploration.</p><p><strong>Aim: </strong>This study aims to investigate the association between psychological distress (anxiety, depression and stress) and fatigue in women with PCOS. In addition, it explores whether sleep disturbances show any association with fatigue, providing insights into factors that may contribute to fatigue severity in this population.</p><p><strong>Settings and design: </strong>This is a cross-sectional pilot study conducted in a university outpatient department.</p><p><strong>Materials and methods: </strong>A total of 52 women, diagnosed with PCOS were included in this cross-sectional study. Data collection involves scoring of fatigue by administering the fatigue severity scale (FSS) and insomnia severity index (ISI) for insomnia and components of psychological distress (stress, depression and anxiety) using the Depression Anxiety Stress Scale-21.</p><p><strong>Statistical analysis used: </strong>Data were analysed using IBM SPSS version 25. Pearson correlation coefficients were used to explore bivariate relationships between fatigue and independent variables. Statistical significance was set at <i>P</i> < 0.05.</p><p><strong>Results: </strong>The findings revealed significant associations between FSS and psychological distress, particularly anxiety (<i>r</i> = 0.507, <i>P</i> = 0.038) and depression (<i>r</i> = 0.595, <i>P</i> = 0.012). In addition, insomnia (ISI) showed the strongest correlation with fatigue (<i>r</i> = 0.705, <i>P</i> = 0.002), indicating that sleep disturbances may further exacerbate fatigue in women with PCOS.</p><p><strong>Conclusion: </strong>This study highlights a strong association between psychological distress, insomnia and fatigue in women with PCOS. The findings suggest that higher levels of anxiety, depression and sleep disturbances contribute significantly to fatigue severity, emphasising the need for integrated psychological and sleep management strategies in PCOS care. Further research with larger cohorts and longitudinal designs is necessary to establish causal relationships and develop targeted interventions for improving overall well-being in this population.</p>","PeriodicalId":15975,"journal":{"name":"Journal of Human Reproductive Sciences","volume":"18 2","pages":"113-117"},"PeriodicalIF":1.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12306717/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Severe Early-onset Ovarian Hyperstimulation Syndrome with Isolated Pleural Effusion following Agonist Trigger in Gonadotropin-releasing Hormone Antagonist Cycle: A Case Report.","authors":"Pooja Dhir Bhayana, Priyanka Singh, Shruti Passan","doi":"10.4103/jhrs.jhrs_39_25","DOIUrl":"10.4103/jhrs.jhrs_39_25","url":null,"abstract":"<p><p>The use of gonadotrophin-releasing hormone (GnRH) agonist trigger in GnRH antagonist cycles in <i>in vitro</i> fertilisation (IVF) with elective freeze-all strategy is known to practically eliminate the risk of severe ovarian hyperstimulation syndrome (OHSS). Still, there are few case reports of early-onset severe OHSS development after agonist use for final oocyte maturation. This patient presented in an emergency on post-Ovum pickup (OPU) day 5 with complaints of cough with chest pain. Her ultrasound chest revealed bilateral massive pleural effusion right > left and required pleural tap on day 7 post-OPU. On Ultrasound abdomen, she was found to have B/L enlarged ovaries consistent with post-OPU status with minimal ascites. She was managed conservatively and discharged in stable condition. This case report emphasises that severe OHSS can develop even after agonist trigger with the segmentation of IVF. The best strategy to prevent OHSS is to keep the stimulation to lower side in patients at risk and take all due precautions to prevent the same.</p>","PeriodicalId":15975,"journal":{"name":"Journal of Human Reproductive Sciences","volume":"18 2","pages":"125-128"},"PeriodicalIF":1.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12306716/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Non-invasive Preimplantation Genetic Testing-Aneuploidy Using Spent Culture Media and its Concordance with Trophectoderm Biopsy: A Proof of Concept and Validation Study.","authors":"Neeta Singh, Ankita Sethi, Lata Rani, Monika Saini, Ritu Gupta, Neena Malhotra, Reeta Mahey","doi":"10.4103/jhrs.jhrs_192_24","DOIUrl":"10.4103/jhrs.jhrs_192_24","url":null,"abstract":"<p><strong>Background: </strong>While trophectoderm (TE) biopsy with next-generation sequencing (NGS) remains the gold standard for preimplantation genetic testing for aneuploidy (PGT-A), the discovery of cell-free DNA (cfDNA) in spent culture media (SCM) has sparked interest in non-invasive PGT-A (NiPGT-A) as a potential alternative.</p><p><strong>Aim: </strong>The study was conducted to assess the feasibility of cell cfDNA from SCM as a tool for NiPGT-A in patients undergoing IVF for advanced age, repeated implantation failure or severe male factor infertility.</p><p><strong>Settings and design: </strong>This is a prospective study where a total of 44 embryos having TE biopsy for aneuploidy testing and their respective SCM collected at day 5/6 were analysed.</p><p><strong>Materials and methods: </strong>All aneuploid blastocysts (WB) were subjected to DNA extraction and amplification using Sureplex DNA amplification system followed by library preparation using VeriSeq™ PGS Library Prep kit and sequencing on MiSeq (Illumina, California, USA).</p><p><strong>Statistical analysis used: </strong>Copy Number Variation visualisation and analysis were carried out using BlueFuse Multi Software (Illumina). The statistical data were analysed by STATA version 14.</p><p><strong>Results: </strong>Informative results were obtained in 36/44 (81.2%) SCM samples. The reads were analysable in 26 paired (SCM and TE biopsy) samples. Concordant NGS results for both TE biopsy and SCM sample were obtained in 17/26 (65.38%) embryos. The per chromosome concordance rate was 85.13% (487/572) and the sex chromosome concordance rate was 73% (19/26). The sensitivity and specificity of NiPGT-A were 66.6% and 60%, respectively. On comparing the ploidy concordance rate, poor morphology embryos had better, but not statistically significant concordance rate (83.33%) as compared to good morphology embryos (50%, <i>P</i> = 0.16). Although not significant, day 6 embryos had better per chromosome as well as sex chromosome concordance rate as compared to day 5 embryos.</p><p><strong>Conclusion: </strong>Aneuploidy testing using cf DNA in SCM is a promising technique but needs more research on larger cohort size to improve the sensitivity, specificity and concordance rate.</p>","PeriodicalId":15975,"journal":{"name":"Journal of Human Reproductive Sciences","volume":"18 2","pages":"70-79"},"PeriodicalIF":1.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12306720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel SUN5 Mutation Associated with Acephalic Spermatozoa Syndrome: A Cross-sectional Study.","authors":"Sarvenaz Alinia, Fatemeh Eskandari Chenari, Seyedeh-Hanieh Hosseini, Alemeh Rafaee, Amaneh Javid, Mohammad Ali Sadighi Gilani, Marjan Sabbaghian","doi":"10.4103/jhrs.jhrs_37_25","DOIUrl":"10.4103/jhrs.jhrs_37_25","url":null,"abstract":"<p><strong>Background: </strong>The <i>SUN5</i> gene encodes a testis-specific protein required for sperm head-tail connection during spermiogenesis. The <i>SUN5</i> gene has an established role in the acephalic spermatozoa syndrome (ASS) defect recognised by headless tails, spermatozoa with disrupted head-tail junction and also a few tailless heads in semen.</p><p><strong>Aim: </strong>This study aims to evaluate the genetic variants of all exons of the <i>SUN5</i> gene and the protein expression in 10 men with ASS.</p><p><strong>Settings and design: </strong>This cross-sectional study was conducted on 10 infertile men with ASS as a case group and 10 men with normal spermogram as a control group referred to the Royan institute between 2015 and 2020.</p><p><strong>Materials and methods: </strong>Polymerase chain reaction and Sanger sequencing were performed on DNA extracted from patients' peripheral blood. In addition, immunocytochemistry and western blotting were accomplished to evaluate the SUN5 protein expression in the patient carrying a distinct exonic variation.</p><p><strong>Statistical analysis used: </strong>No statistical analysis was needed to be done in this survey.</p><p><strong>Results: </strong>Sequencing outcomes represented one homozygous missense mutation (c.1073G>A [p.Arg358Gln]) in exon 13 of the <i>SUN5</i> gene in one patient with 98% acephalic spermatozoa in the total sperm population. This mutation did not exist in the control group. SUN5 protein expression was completely undetectable in the patient with c.1073G>A.</p><p><strong>Conclusion: </strong>Based on current results, it could be concluded that c.1073G>A detected mutations in the <i>SUN5</i> gene could alter the SUN5 protein expression and lead to male infertility due to ASS.</p>","PeriodicalId":15975,"journal":{"name":"Journal of Human Reproductive Sciences","volume":"18 2","pages":"105-112"},"PeriodicalIF":1.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12306721/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Commentary.","authors":"Mohan S Kamath","doi":"10.4103/jhrs.jhrs_120_25","DOIUrl":"https://doi.org/10.4103/jhrs.jhrs_120_25","url":null,"abstract":"","PeriodicalId":15975,"journal":{"name":"Journal of Human Reproductive Sciences","volume":"18 2","pages":"59"},"PeriodicalIF":1.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12306712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strategies to Improve Assisted Reproductive Technique Outcomes in Women with Adenomyosis: A Narrative Review.","authors":"Ruma Satwik, Nitisha Verma, Vijaita Thakur","doi":"10.4103/jhrs.jhrs_100_25","DOIUrl":"10.4103/jhrs.jhrs_100_25","url":null,"abstract":"<p><p>Adenomyosis is a complex, heterogeneous condition characterised by the development of ectopic endometrial glandular and stromal tissue within the myometrium. Surrounding it, there is reactionary fibrosis, hyperplasia and hypertrophy of the surrounding smooth muscle cells. This induces inflammatory changes in the eutopic endometrium, which alters the molecular environment at the time of implantation. Evidence from systematic reviews of observational studies has suggested a lowering of implantation and live birth rates and an increase in miscarriage rates in assisted reproductive technique (ART), particularly with diffuse adenomyosis involving the inner myometrium and junctional zone. This review describes and evaluates the various medical and surgical strategies employed to improve ART outcomes. These strategies are derived from observational data, and none have been tested through randomised controlled trials. In addition, there is a need to homogenise the reporting of adenomyosis on ultrasonography and stratify adenomyosis populations based on disease burden when determining the efficacy of various interventions.</p>","PeriodicalId":15975,"journal":{"name":"Journal of Human Reproductive Sciences","volume":"18 2","pages":"60-69"},"PeriodicalIF":1.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12306718/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Report on the Effect of White and Black Truffle Extracts on Rat Semen Parameters <i>In vitro</i>.","authors":"Victoria Nikolaevna Shelkovnikova, Maria Egorovna Dmitrieva, Ekaterina Vladimirovna Malygina, Natalia Alexandrovna Imidoeva, Alexander Yurievich Belyshenko, Maria Mikhailovna Morgunova, Anfisa Alexandrovna Vlasova, Tamara Yurievna Telnova, Anna Alexandrovna Batalova, Olga Evgenievna Lipatova, Tatyana Nikolaevna Vavilina, Angelika Sergeevna Listopad, Olga Vladimirovna Yurlova, Elena Igorevna Martynova, Denis Victorovich Axenov-Gribanov","doi":"10.4103/jhrs.jhrs_35_25","DOIUrl":"10.4103/jhrs.jhrs_35_25","url":null,"abstract":"<p><strong>Background: </strong>Truffle extracts possess the unique properties that make them potentially valuable for medicinal purposes. The use of truffles in reproductive medicine may be of particular interest for developing medications with both spermatozoa-activating and contraceptive effects.</p><p><strong>Aim: </strong>Our experiment evaluated the influence of extracts of white and black truffle mushrooms on rat spermatozoa.</p><p><strong>Settings and design: </strong>Animal model.</p><p><strong>Materials and methods: </strong>The study utilized 28 samples of wild truffle fruiting bodies. In the experiment, we used ejaculate from male Wistar rats (<i>n</i> = 10, 8-12 months). Sperm samples from ten male rats were incubated at 37°C in a 96-well plate under the three conditions: control (methanol evaporated), concentrated truffle extract (25 µL), and diluted extract (1:6 ratio). After solvent evaporation, 150 µL of ejaculate was added to each well. Sixteen sperm parameters were measured using an automated analysis system after 1, 3, and 6 h of incubation.</p><p><strong>Statistical analysis used: </strong>The methods should be adequately detailed or referenced to other work. The statistical processing was performed in the Past software (V4.03) using the ANOVA analysis of variance with the Mann-Whitney test. Differences between the mean values of the parameters were considered statistically significant at <i>P</i> ≤ 0.05.</p><p><strong>Results: </strong>Research has shown that extracts from black and white truffles related to Tuber sp. have a stimulating effect on spermatozoa. Curvilinear velocity and beat cross-frequency also increased by 48% and 50%, respectively. Linearity showed a significant increase by 56% and straightness by 48%. This could be useful in the development of drugs to enhance sperm activity and lifespan. In addition, extracts from black truffles have been found to have a negative impact on spermatozoa, which could be relevant for developing new contraceptive drugs.</p><p><strong>Conclusion: </strong>Our study demonstrated the influence of methanol extracts of Tuber sp. mushrooms on male gametes of rats <i>in vitro</i>.</p>","PeriodicalId":15975,"journal":{"name":"Journal of Human Reproductive Sciences","volume":"18 2","pages":"118-124"},"PeriodicalIF":1.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12306714/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}