Journal of Immunology Research最新文献

{"title":"Astaxanthin Mitigates Dextran Sulfate Sodium-Induced Colitis in Mice by Repairing the Intestinal Barrier, Regulating Specific Intestinal Flora, and Reducing Inflammatory Cell Infiltration.","authors":"Guangzhe Shen, Du Yu, Longfei Xu, Tiezheng Yang","doi":"10.1155/jimr/3381950","DOIUrl":"10.1155/jimr/3381950","url":null,"abstract":"<p><p>As a dietary supplement for humans and animals, Astaxanthin (Ax) is widely believed to possess antioxidant and anti-inflammatory properties. In this study, we attempted to evaluate the protective effects of Ax on dextran sodium sulfate (DSS)-induced colitis in mice and the underlying molecular mechanism. Our results suggested that Ax significantly reduced the severity of DSS-induced colitis in mice, as evidenced by increased colon length, decreased disease activity index (DAI), and attenuated inflammatory factors. In addition, Ax significantly increased the diversity of gut microbiota in mice with colitis, remodeled the microbial composition, promoted the production of beneficial bacteria (e.g., Lactobacillaceae), and inhibited the production of harmful bacteria (e.g., Lachnospiraceae and Muribaculaceae). In conclusion, Ax alleviated DSS-induced colitis by maintaining the intestinal barrier and regulating intestinal microbes.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2025 ","pages":"3381950"},"PeriodicalIF":3.6,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12393947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144956967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"miR-214-3p Deficiency Enhances Caspase-1-Dependent Pyroptosis of Microglia in White Matter Injury\".","authors":"","doi":"10.1155/jimr/9826132","DOIUrl":"https://doi.org/10.1155/jimr/9826132","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1155/2022/1642896.].</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2025 ","pages":"9826132"},"PeriodicalIF":3.6,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367391/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144956905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intradermally Administered Retinoic Acid or Vitamin D3-Loaded Liposomes Induce Tolerogenic Skin Dendritic Cells.","authors":"Noémi Anna Nagy, Sanne G Celant, Toni M M van Capel, Rinske Sparrius, Fernando Lozano Vigario, Ronald van Ree, Bram Slütter, Teunis B H Geijtenbeek, Sander W Tas, Esther C de Jong","doi":"10.1155/jimr/2208155","DOIUrl":"10.1155/jimr/2208155","url":null,"abstract":"<p><p>In vivo targeting of dendritic cells (DCs) with nanocarriers containing tolerogenic adjuvants is an attractive strategy to dampen inflammation. Here, we used ex vivo skin vaccination to examine the effect of intradermal injection of liposomes loaded with the tolerogenic adjuvants all-trans retinoic acid (RA) and vitamin D3 (VD3). We investigated the effect of intradermal liposome injection on skin DCs and the skin DC-induced T cell response. Our study shows that intradermal injection of RA or VD3-loaded anionic phospholipid 1,2-distearoyl-sn-glycero-3-phosphoglycerol (DSPG) liposomes selectively induces CD14+ dermal DC (DDC) migration while reducing migration of CD1a dim DDCs. Migrated CD14+ DDCs displayed a partially immature phenotype. RA or VD3 liposome-treated CD1a dim DDCs exhibited reduced expression of maturation markers and induced expression of coinhibitory immunoglobulin-like transcript 3 (ILT3). VD3 liposome-treated CD14+ DDCs, as well as, CD1a dim DDCs, exhibited reduced expression of maturation markers, induction of coinhibitory molecules ILT3, and programmed death-ligand 1 (PD-L1). Migrated DCs from RA or VD3 liposome-injected skin differentiated naïve CD4+ T cells into FoxP3+ CD127 low and ICOS+ Tregs, expressing functional regulatory markers. Thus, our findings provide further substantiation for in vivo DC-modulating vaccines with tolerogenic liposomes as a putative clinical therapy for autoimmune diseases and allergies.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2025 ","pages":"2208155"},"PeriodicalIF":3.6,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12339165/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144821529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Impaired Proliferation of CD8⁺ T Cells Stimulated with Monocyte-Derived Dendritic Cells Previously Matured with Thapsigargin-Stimulated LAD2 Human Mast Cells\".","authors":"","doi":"10.1155/jimr/9807058","DOIUrl":"https://doi.org/10.1155/jimr/9807058","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1155/2024/5537948.].</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2025 ","pages":"9807058"},"PeriodicalIF":3.6,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12328042/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-Cell Data and Weighted Correlation Network Analysis Revealed the Regulatory Mechanisms of Macrophages in Carotid Plaques.","authors":"Yakun Ding, Xiaoyang Niu, Peng Guo, Bing Wang","doi":"10.1155/jimr/9987367","DOIUrl":"10.1155/jimr/9987367","url":null,"abstract":"<p><p><b>Background:</b> Macrophages play a critical role in carotid plaque. Understanding the mechanisms of carotid plaque formation based on macrophage heterogeneity could provide valuable insights for clinical intervention. <b>Methods:</b> Single-cell transcriptome and bulk RNA-seq data of carotid plaque were obtained from public databases. Weighted gene correlation network analysis (WGCNA) identified gene modules linked to unstable plaques. Macrophage marker genes were intersected with module genes of WGCNA, followed by using randomForest and LASSO regression to pinpoint key genes. Quantitative real-time PCR (qRT-PCR) and Western blot were used to verify the regulation of key genes at the cellular level. The correlation between the key genes and inflammatory phenotypes was examined by single-sample gene set enrichment analysis (ssGSEA). <b>Results:</b> Single-cell clustering revealed major cellular subpopulations, with elevated macrophage infiltration in carotid plaque. Six key macrophage-associated genes (<i>ADPGK, ATP6V1F, CX3CR1, MYO9B, RNF135</i>, and <i>SLC7A8</i>) were discovered. The qRT-PCR results demonstrated upregulation of <i>ADPGK, ATP6V1F</i>, and <i>RNF135</i> genes in vascular smooth muscle cells (VSMCs) treated with oxidized low-density lipoprotein (ox-LDL), except for <i>CX3CR1</i>, which was downregulated. Protein expression results showed that expressions of <i>ADPGK, ATP6V1F, RNF135</i>, and <i>SLC7A8</i> were significantly elevated in the ox-LDL-VSMC group. In addition, most of the immune cells showed significant differences between the unstable arterial plaque group and the control group. <b>Conclusion:</b> This study discovered potential biomarkers that affected carotid plaque progression and macrophage regulation at the single-cell level, and examined their regulatory roles in immune regulation, programed cell death (PCD), and inflammatory factor modulation.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2025 ","pages":"9987367"},"PeriodicalIF":3.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12303652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epigenetic Regulation of B Cell Memory Formation: A Poised Model for B Cell Epigenetic Reprograming.","authors":"Carlos Valverde-Hernandez","doi":"10.1155/jimr/9328523","DOIUrl":"10.1155/jimr/9328523","url":null,"abstract":"<p><p>The formation of B cell immunological memory happens after the first encounter with a pathogen. At the germinal center (GC), B cells experience complex transcriptional and epigenetic transitions to differentiate into memory B cells (MBCs) and plasma cells (PCs). In particular, the differentiation of GC B cells into MBCs has been poorly understood, and no clear conclusions on the signals and transcription factors leading to this cell fate have been identified. Recent discoveries in epigenetics and immune memory have elucidated the essential role of epigenetic regulators in establishing the memory B cell (MBC) fate. DNA methylation regulators, histone modifiers, noncoding RNAs (ncRNAs), and chromatin remodelers orchestrate a dynamic reprograming of the MBC phenotype. Positive and negative epigenetic regulators of the B cell program collaborate at each differentiation stage and allow for complex chromatin topology rearrangements and dynamic exposure to transcription and translation. Following MBC fate determination at the GC, the acquired epigenetic modifications induce a poised regulatory state where genes are epigenetically marked to remain transcriptionally inactive, but primed for rapid activation upon stimuli. Thus, a poised epigenetic control over gene expression governs MBC formation and a novel model of epigenetic reprograming is proposed. This model provides a novel perspective on how the B cell fate is determined in the GC and memory is formed, offering insights for improved vaccination and therapeutical approaches.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2025 ","pages":"9328523"},"PeriodicalIF":3.6,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12289371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144707761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Transcriptomic Analysis Provided Diagnostic and Pathophysiological Insights for Epilepsy.","authors":"Shuang Li, Zhigang Wang, Yake Zheng, Yunqing Ma, Zhi Huang, Yajun Lian","doi":"10.1155/jimr/5925485","DOIUrl":"10.1155/jimr/5925485","url":null,"abstract":"<p><p><b>Background:</b> Epilepsy is a common neurological disorder involving multiple genes and molecular pathways. Study of differentially expressed genes (DEGs) and hub genes related to epilepsy can help reveal the pathophysiologic basis and improve potential diagnostic and therapeutic strategies. <b>Methods:</b> Transcriptome data of two epilepsy datasets (GSE143272 and GSE32534) and single-cell sequencing data (GSE201048) were collected from the Gene Expression Omnibus (GEO) database. Differential expression analysis was performed using Limma R package, and the hub genes were identified and analyzed utilizing STRING database and Cytoscape software. The clusterProfiler R package was used to perform gene function enrichment analysis and an epilepsy diagnostic model was constructed with the hub genes. The model performance was assessed according to receiver operating characteristic (ROC) curves. <b>Results:</b> Multiple DEGs linked to epilepsy were identified and 20 common DEGs between the two datasets were revealed. Eleven hub genes closely associated with epilepsy were identified by protein-protein interaction (PPI) network analysis. CD3D, CD3G, CTSW, and JCHAIN were consistently expressed in the GSE143272 and GSE32534 datasets and all showed a low expression in epilepsy samples. In particular, the diagnostic model developed with the four genes demonstrated a strong discriminatory ability in both datasets (all area under curve (AUC) > 0.7). Functional enrichment and single-cell analysis revealed that these key genes were closely related to T cell function, suggesting that they may play an important role in the immune regulation of epilepsy. <b>Conclusion:</b> This study successfully identified four key genes linked to epilepsy, contributing to the molecular diagnosis of epilepsy.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2025 ","pages":"5925485"},"PeriodicalIF":3.5,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Poxvirus Vectors Activate Human NK and MAIT Cells in a Type I Interferon, IL-18, and Monocyte-Dependent Manner.","authors":"Kombo F N'guessan, Zhanna Shubin, Kawthar Machmach, Johan K Sandberg, Julie A Ake, Sandhya Vasan, Michael A Eller, Dominic Paquin-Proulx","doi":"10.1155/jimr/1203141","DOIUrl":"10.1155/jimr/1203141","url":null,"abstract":"<p><p>Recombinant poxviruses have been extensively studied as vaccine vectors, yet the specific mechanisms by which they engage the immune system remain incompletely understood. ALVAC is a poxviral vector that was a component of the HIV vaccine used in the Thai RV144 trial, showing modest efficacy in reducing HIV acquisition. Here, we show that in vitro ALVAC-HIV infection of peripheral blood mononuclear cells (PBMCs) activates natural killer (NK) and mucosal-associated invariant T (MAIT) cells. This activation was partially dependent on monocytes, cGAS sensing, and production of IL-18 and type I IFN. Furthermore, ALVAC-HIV-mediated activation of NK and MAIT cells contributed to the activation of B cells. Modified vaccinia Ankara (MVA), another poxviral vector used for prevention of smallpox and mpox, similarly activated NK and MAIT cells. Overall, this suggests a conserved mechanism by which NK and MAIT cells could contribute to the immunogenicity of poxviral vectors.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2025 ","pages":"1203141"},"PeriodicalIF":3.5,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12276059/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"4-Phenyl Butyric Acid (4-PBA) Suppresses Neutrophil Recruitment in a Murine Model of Acute Perinatal Inflammation.","authors":"Christian Gille, Maylis Jungwirth, Silvia Pezer, Stefanie Dietz-Ziegler, Natascha Köstlin-Gille, Trim Lajqi","doi":"10.1155/jimr/2438058","DOIUrl":"10.1155/jimr/2438058","url":null,"abstract":"<p><p>Neutrophils are the first immune cells to be recruited to the site of infection and deregulated activation is linked to adverse outcome, especially in premature neonates. Dampening neutrophil activity may therefore be a means of preventing acute and chronic inflammatory diseases; however, little is known about potential drugs to modulate neutrophil activity. 4-Phenyl butyric acid (4-PBA) is a clinically used drug, which acts as a chemical chaperone to inhibit endoplasmic reticulum (ER) stress and to suppress immune activation. Here, we investigated the potential of 4-PBA to regulate neutrophil-mediated inflammation and specifically the recruitment cascade of neutrophils. We found that 4-PBA suppressed perinatal neutrophil recruitment cascade as assessed by fetal intravital microscopy (IVM), as well as transmigration of neutrophils through the endothelial compartment via the inositol-requiring enzyme (IRE)-1α/extracellular signal-regulated kinase (ERK) 1/2 signaling pathway. Likewise, 4-PBA promoted an anti-inflammatory phenotype by suppressing the release of pro-inflammatory mediators in bone marrow neutrophils and endothelial cells in vitro. Taken together, our data indicate that 4-PBA can exert anti-inflammatory effects by limiting excessive neutrophil infiltration into inflamed tissues, thereby holding significant therapeutic potential in managing various inflammatory diseases.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2025 ","pages":"2438058"},"PeriodicalIF":3.5,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12259320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Multifaceted Role of the IL-2 Cytokine Family in Melanoma: Mechanisms, Therapeutic Implications, and Immune Modulation.","authors":"Mona Daghaighei, Samaneh Dodge, Soheil Bolandi, Boutros Youssef, Niket Attarde, Moein Maddahi, Maryam Mostofi, Reza Morovatshoar, Mehrnaz Mostafavi, Dejbakht Majid, Mohammadamin Joulani, Pegah Tamimi, Malihe Sharafi, Qumars Behfar, Yasaman Ghodsi Boushehri, Alireza Azani","doi":"10.1155/jimr/8890939","DOIUrl":"10.1155/jimr/8890939","url":null,"abstract":"<p><p><b>Background and Objective:</b> Melanoma is a complex malignancy where the interplay between immune cells, cytokines, and the tumor microenvironment (TME) significantly influences disease progression and patient outcomes. This review explores the involvement of the interleukin-2 (IL-2) cytokine family in both the development and therapeutic approaches for melanoma. <b>Methods:</b> A narrative literature review was conducted, synthesizing findings from studies on immune cell behavior, cytokine functions, and their implications in melanoma and other cancers. This narrative review emphasizes the roles of immune cells and cytokines in both promoting and inhibiting tumor growth. <b>Results:</b> Neutrophils, influenced by tumor-derived cytokines, can adopt phenotypes that either inhibit or promote tumor growth. B cells in the TME often correlate with better survival, although their regulatory forms can suppress immune responses. Tissue-resident memory T cells (TRM cells) are crucial for antitumor immunity, particularly in response to immune checkpoint inhibitors (ICIs). Dendritic cells (DCs) are vital for antigen presentation, yet their function can be compromised in melanoma. Macrophages frequently support tumor growth through immunosuppressive actions. The IL-2 cytokine family, including IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21, plays diverse roles in immune regulation. These cytokines are involved in T-cell proliferation, B-cell differentiation, and modulation of other immune responses, influencing both tumor progression and the effectiveness of immunotherapies. <b>Conclusions:</b> Immune cells and cytokines are pivotal in the pathogenesis, progression, and immunotherapy of melanoma. Understanding their complex roles offers insights into potential therapeutic strategies, highlighting the importance of targeted immunotherapies in treating melanoma and possibly other cancers. Additional studies are required to clarify the precise mechanisms and interactions occurring within the TME to enhance treatment strategies.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2025 ","pages":"8890939"},"PeriodicalIF":3.6,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12240660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144600693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}