Journal of Immunology Research最新文献

{"title":"Broadly Reactive SARS-CoV-2-Specific T-Cell Response and Participation of Memory B and T Cells in Patients with Omicron COVID-19 Infection.","authors":"Pragya D Yadav,&nbsp;Rima R Sahay,&nbsp;Sukeshani Salwe,&nbsp;Diptee Trimbake,&nbsp;Prasad Babar,&nbsp;Gajanan N Sapkal,&nbsp;Gururaj R Deshpande,&nbsp;Kiran Bhise,&nbsp;Anita M Shete,&nbsp;Priya Abraham,&nbsp;Anuradha S Tripathy","doi":"10.1155/2023/8846953","DOIUrl":"10.1155/2023/8846953","url":null,"abstract":"<p><p>January 2022 onward, India witnessed a sudden increase in Omicron COVID-19 infections, having a mild course that prompted us to identify the key host factors/immune molecules modulating disease course/outcomes. The current study evaluated the percentages of lymphocyte subsets by flowcytometry, SARS-CoV-2 specific T-cell immune response by ELISPOT, estimation of plasma cytokine/chemokine levels on a Bio-plex Multiplex Immunoassay System and anti-SARS-CoV-2 IgG levels by enzyme-linked immunosorbent assay in 19 mild Omicron infected patients, 45 mild SARS-CoV-2 (2020) patients and 36 uninfected controls from India. Natural killer cells, B and memory B cells were high in vaccinated and total Omicron-infected patients groups compared to the mild SARS-CoV-2 (2020) patient group, while CD8⁺ T cells were high in total Omicron-infected patients group compared to the uninfected control group (<i>p</i> < 0.05 each). Omicron-infected patients had T-cell response against SARS-CoV-2 whole virus, S1 proteins (wild type and delta variant) in 10 out of 17 (59%), 10 out of 17 (59%), and 8 out of 17 (47%), respectively. The current study of Omicron-infected patients elucidates broadly reactive antibody, T-cell response, and participation of memory B and T cells induced by vaccination/natural infection. The limited effect of Omicron's mutations on T-cell response is suggestive of protection from severity. Pro-inflammatory IL-6, IFN-<i>γ</i>, chemokines CCL-2, CCL-3, CCL-4, CCL-5, and IL-8 as potential biomarkers of Omicron infection may have future diagnostic importance. The cellular immune response data in Omicron-infected patients with parental Omicron lineage could serve as a starting point to define the readouts of protective immunity against circulating Omicron subvariants.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2023 ","pages":"8846953"},"PeriodicalIF":4.1,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10597734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50161849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex-Specific Immune Responses to Seasonal Influenza Vaccination in Diabetic Individuals: Implications for Vaccine Efficacy.","authors":"Anirban Sengupta,&nbsp;Noha Al-Otaibi,&nbsp;Jorma Hinkula","doi":"10.1155/2023/3111351","DOIUrl":"10.1155/2023/3111351","url":null,"abstract":"<p><p>Seasonal influenza vaccination has different implications on the immune response depending on the comorbidities. Diabetes is one such critical disease that increases the patient's susceptibility to influenza and suppresses vaccine efficacy and immunity. The sex of the individuals also plays a definitive role in the immune responses to both the vaccine and the infection. This study aims to understand the efficacy of the seasonal vaccine against influenza in diabetic groups and undergoing immune mechanisms in different sexes (females and males). In this study, we are reporting about a switching of the immune response of the infected and vaccinated diabetic females towards stronger Th1/Th17 responses with suppressed humoral immunity. They show increased cDC1, enhanced proinflammatory activities within T cells, CD8T activation, Th17 proliferation, and the majority of IgG2 antibody subtypes with reduced neutralization potential. Males with diabetes exhibit enhanced humoral Th2-immunity than the nondiabetic group. They exhibit higher cDC2, and DEC205 levels within them with an increase in plasma B lymphocytes, higher IgG1 subtypes in plasma cells, and influenza-hemagglutinin-specific IgG titer with stronger virus neutralization potential. Males with diabetes recovered better than the females as observed from the changes in their body weight. This study highlights the critical immune mechanisms and sex-specific swapping of their preferred immune response pathways against influenza after vaccination during diabetes. We propose a need for a sex-specific customized vaccine regimen to be implemented against influenza for individuals having diabetes to exploit the manifested strength and weakness in their protective immunity.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2023 ","pages":"3111351"},"PeriodicalIF":4.1,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10597737/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50161850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review of Antibiotic Efficacy in COVID-19 Control.","authors":"Hamidreza Hekmat, Aziz Rasooli, Zeinab Siami, Kauthar Amir Rutajengwa, Zahra Vahabi, Fatemeh Alsadat Mirzadeh","doi":"10.1155/2023/6687437","DOIUrl":"10.1155/2023/6687437","url":null,"abstract":"<p><p>Severe acute respiratory disease is associated with chronic secondary infections that exacerbate symptoms and mortality. So far, many drugs have been introduced to treat this disease, none of which effectively control the coronavirus. Numerous studies have shown that mitochondria, as the center of cell biogenesis, are vulnerable to drugs, especially antibiotics. Antibiotics were widely prescribed during the early phase of the pandemic. We performed a literature review to assess the reasons, evidence, and practices on the use of antibiotics in coronavirus disease 2019 (COVID-19) in- and outpatients. The current research found widespread usage of antibiotics, mostly in an empirical context, among COVID-19 hospitalized patients. The effectiveness of this approach has not been established. Given the high death rate linked with secondary infections in COVID-19 patients and the developing antimicrobial resistance, further study is urgently needed to identify the most appropriate rationale for antibiotic therapy in these patients.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2023 ","pages":"6687437"},"PeriodicalIF":3.5,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49678104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential Production of Midkine and Pleiotrophin by Innate APCs upon Stimulation through Nucleic Acid-Sensing TLRs.","authors":"Elias A Said,&nbsp;Sumaya Al-Dughaishi,&nbsp;Wadha Al-Hatmi,&nbsp;Iman Al-Reesi,&nbsp;Mohammed S Al-Balushi,&nbsp;Atika Al-Bimani,&nbsp;Juma Z Al-Busaidi,&nbsp;Marwa Al-Riyami,&nbsp;Murtadha Al-Khabori,&nbsp;Salam Al-Kindi,&nbsp;Francesco A Procopio,&nbsp;Shadia Al-Sinawi,&nbsp;Aliyaa Al-Ansari,&nbsp;Crystal Y Koh,&nbsp;Khalid Al-Naamani,&nbsp;Ali A Al-Jabri","doi":"10.1155/2023/7944102","DOIUrl":"10.1155/2023/7944102","url":null,"abstract":"<p><p>Midkine (MK) and pleiotrophin (PTN) belong to the same family of cytokines. They have similar sequences and functions. Both have important roles in cellular proliferation, tumors, and diseases. They regulate and are expressed by some immune cells. We have recently demonstrated MK production by some human innate antigen-presenting cells (iAPCs), i.e., monocyte-derived dendritic cells (MDDCs) and macrophages stimulated through Toll-like receptor (TLR)-4, and plasmacytoid dendritic cells (pDCs) stimulated through TLR 7. While PTN production was only documented in tissue macrophages. TLRs 3, 7, 8, and 9 are nucleic acid sensing (NAS) TLRs that detect nucleic acids from cell damage and infection and induce iAPC responses. We investigated whether NAS TLRs can induce MK and PTN production by human iAPCs, namely monocytes, macrophages, MDDCs, myeloid dendritic cells (mDCs), and pDCs. Our results demonstrated for the first time that PTN is produced by all iAPCs upon TLR triggering (<i>p</i> < 0.01). IAPCs produced more PTN than MK (<i>p</i> < 0.01). NAS TLRs and iAPCs had differential abilities to induce the production of MK, which was induced in monocytes and pDCs by all NAS TLRs (<i>p</i> < 0.05) and in MDDCs by TLRs 7/8 (<i>p</i> < 0.05). TLR4 induced a stronger MK production than NAS TLRs (<i>p</i> ≤ 0.05). Monocytes produced higher levels of PTN after differentiation to macrophages and MDDCs (<i>p</i> < 0.05). The production of MK and PTN differs among iAPCs, with a higher production of PTN and a selective induction of MK production by NAS TLR. This highlights the potentially important role of iAPCs in angiogenesis, tumors, infections, and autoimmunity through the differential production of MK and PTN upon TLR triggering.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2023 ","pages":"7944102"},"PeriodicalIF":4.1,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10578979/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41235902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of the IL-33/ST2 Axis in CpG-Induced Macrophage Activation Syndrome.","authors":"Yuanji Dong,&nbsp;Rongfen Gao,&nbsp;Kailin He,&nbsp;Jixin Zhong,&nbsp;Lingli Dong","doi":"10.1155/2023/2689360","DOIUrl":"10.1155/2023/2689360","url":null,"abstract":"<p><strong>Background: </strong>Macrophage activation syndrome (MAS) is a fatal inflammatory condition, which is often associated with the elevation of multiple proinflammatory cytokines and multiple organ dysfunction. Previous studies have shown that ST2 contributes to T cell overactivation and plays a detrimental role in mouse models of primary hemophagocytic lymphohistiocytosis. The purpose of this study was to investigate the role of the IL-33/ST2 axis in a mouse model of MAS induced by repeated injections of cytosine-phosphate-guanine (CpG).</p><p><strong>Methods: </strong>Serum cytokines were determined using the cytometric bead array by flow cytometry. IL-33 and ST2 were detected by immunohistochemistry and real-time quantitative PCR in the liver and spleen of mice. CD3 and F4/80 in the liver were detected by immunohistochemistry. Inflammatory macrophages and effector memory T lymphocytes were detected by flow cytometry.</p><p><strong>Result: </strong>The CpG-induced MAS model was successfully induced after repeated CpG injections, presenting with hypercytokinemia and hepatosplenomegaly. The numbers of IL-33 positive cells in the liver and spleen decreased significantly, while the expression of ST2 in the liver tended to increase in the mice with MAS. <i>IL-33</i> and <i>St2</i> knockout mice showed similar levels of hepatosplenomegaly, peripheral blood count, and cytokine storm when compared with wild-type (WT) mice after induction of MAS. There were also no significant differences in liver pathology (including inflammatory cell infiltration of CD3 and F4/80) and levels of splenic inflammatory macrophages and effector memory T cells between the WT and knockout mice.</p><p><strong>Conclusion: </strong>These results suggested that IL-33 decreased in the liver and spleen tissues of MAS mice. Further results suggest that <i>IL-33</i> and <i>St2</i> knockout mice have no treatment potential in CpG-induced MAS. Thus, the IL-33/ST2 axis has little effect on the prognosis of CpG-induced MAS.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2023 ","pages":"2689360"},"PeriodicalIF":4.1,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10569892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41235903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Value of CD25, CD69, and CD134 on Tuberculosis-Specific Antigen-Stimulated CD4+ T Cells for Tuberculous Pleurisy.","authors":"Hanlu Shi,&nbsp;Liping Yang,&nbsp;Fujie Zhang,&nbsp;Yu Zhou,&nbsp;Yonglie Zhou","doi":"10.1155/2023/5309816","DOIUrl":"10.1155/2023/5309816","url":null,"abstract":"<p><p>Rapid and accurate methods for the diagnosis of tuberculous pleurisy (TP) are urgently needed. Activation markers of tuberculosis (TB)-reactive T cells are considered promising for the diagnosis of active TB (ATB). Different activation indexes may play different roles in the progression of TB, but there are few reports on T cell activation indicators, except for HLA-DR. Hence, we evaluated the expression of early (CD25 and CD69) and late (CD134) activation markers on TB antigen-stimulated CD4+ T cells in populations with different TB infection status and investigated their diagnostic value for ATB, particularly, for TP. Moreover, we compared the differences in the diagnostic efficacy among the indexes from peripheral blood (PB) and pleural fluid (PF) for TP. The expression of each activation marker was significantly increased in TB-infected populations (patients with ATB and latent TB infection vs. healthy individuals; patients with TP vs. non-TP) and was significantly higher in the PF than in the PB of patients with TP. The diagnostic performance of the coexpressed activation markers was superior to that of single expression markers in the differential diagnosis of ATB and non-TB, with CD25+CD134+ showing the best diagnostic efficiency (AUC: 0.93, 95% CI, 0.87-0.99; sensitivity: 86.7%, 95% CI, 72.5%-94.5%; and specificity: 94.0%, 95% CI, 82.5%-98.4%). Except for TB-IGRA, the activation indexes were more accurate than conventional laboratory methods for ATB diagnosis. In addition, the expression of CD25+CD134+ in PB and PF was the best values for differential diagnosis of TP and NTP, with AUCs of 0.87 (95% CI, 0.77-0.96) and 0.95 (95% CI, 0.90-1.00), respectively. Our study provides information on the diagnostic value of different activation markers for TB and shows that the expression of CD25+CD134+ on CD4+ T cells in PF can serve as a potential marker for TP diagnosis.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2023 ","pages":"5309816"},"PeriodicalIF":4.1,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10551431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41134643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alterations in the \"Gut-Liver Axis\" on Rats with Immunological Hepatic Fibrosis.","authors":"Zhaoyao Qi,&nbsp;Xinxin Qi,&nbsp;Yuanhui Xu,&nbsp;Hongguang Sun,&nbsp;Dengfeng Li,&nbsp;Jincun Liu,&nbsp;Meili Cong,&nbsp;Tao Liu","doi":"10.1155/2023/5577850","DOIUrl":"https://doi.org/10.1155/2023/5577850","url":null,"abstract":"<p><p>There remains a lack of standard models that have all the characteristics of human diseases. Especially in immunological hepatic fibrosis, the bovine serum albumin (BSA)-induced liver fibrosis models have the same developmental mechanisms as human liver fibrosis models, but have received little attention. We standardized a BSA-induced liver fibrosis model in rats and thoroughly assessed its pathological characteristics. We also used 16S sequencing to assess homeostasis of the intestinal microflora of rats with BSA-induced liver fibrosis and detected various differential metabolites in the serum of these rats using ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). We observed stable and unambiguous histological changes in liver tissue morphology and remarkably high concentrations of inflammatory markers in the serum of BSA-induced liver fibrosis rats. In keeping with the fact that BSA induction can cause gut microbiota disorders in rats. UHPLC-MS/MS analysis of rat serum samples in positive-ion mode and negative-ion mode revealed 17 and 25 differential metabolites, respectively. Network analysis revealed that phenylalanine or tyrosine metabolites (e.g., PAGln) were the predominant metabolites in the sera of BSA-induced liver fibrosis rats. Taken together, our results suggest that disorders of amino acid metabolism caused by the gut microbiota may play an important role in the progression of immunological hepatic fibrosis.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2023 ","pages":"5577850"},"PeriodicalIF":4.1,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41135497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"sNASP Mutation Aggravates to the TLR4-Mediated Inflammation in SLE by TAK1 Pathway.","authors":"Yatao Bao,&nbsp;Meng Lian,&nbsp;Yong Chen,&nbsp;Xiaotian Gu,&nbsp;Kunyu Cao,&nbsp;Xiaoping Du,&nbsp;Jiyu Ju","doi":"10.1155/2023/4877700","DOIUrl":"https://doi.org/10.1155/2023/4877700","url":null,"abstract":"<p><p>Genetic factors play an important role in the pathogenesis of systemic lupus erythematosus (SLE), and abnormal Toll-like receptor (TLR) signaling pathways are closely related to the onset of SLE. In previous studies, we found that the mutant somatic nuclear autoantigenic sperm protein (sNASP) gene in the mouse lupus susceptibility locus <i>Sle2</i> can promote the development of lupus model mice, but the mechanism is still unclear. Here, we stimulated mouse peritoneal macrophages with different concentrations of lipopolysaccharide. The results showed that sNASP gene mutations can promote the response of the TLR4-TAK1 signaling pathway but have no significant effect on the TLR4-TBK1 signaling pathway. sNASP mutations enhanced TLR4-mediated nuclear factor-<i>κ</i>-gene binding and mitogen-activated protein kinase activation and IL-6, tumor necrosis factor secretion in murine peritoneal macrophages. Collectively, our study revealed the impact of sNASP gene mutation on the sensitivity of TLR4 receptors in mouse peritoneal macrophages and shed light on potential mechanisms underlying inflammation in autoimmune diseases.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2023 ","pages":"4877700"},"PeriodicalIF":4.1,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10533267/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41129773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Super Enhancer Regulatory Gene FYB1 Promotes the Progression of T Cell Acute Lymphoblastic Leukemia by Activating IGLL1.","authors":"Kunlong Zhang,&nbsp;Jun Lu,&nbsp;Fang Fang,&nbsp;Yongping Zhang,&nbsp;Juanjuan Yu,&nbsp;Yanfang Tao,&nbsp;Wenyuan Liu,&nbsp;Lihui Lu,&nbsp;Zimu Zhang,&nbsp;Xinran Chu,&nbsp;Jianwei Wang,&nbsp;Xiaolu Li,&nbsp;Yuanyuan Tian,&nbsp;Zhiheng Li,&nbsp;Qian Li,&nbsp;Xu Sang,&nbsp;Li Ma,&nbsp;Ningling Wang,&nbsp;Jian Pan,&nbsp;Shaoyan Hu","doi":"10.1155/2023/3804605","DOIUrl":"https://doi.org/10.1155/2023/3804605","url":null,"abstract":"<p><strong>Background: </strong>Arising from T progenitor cells, T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematologic malignant tumor, accounting for 15% of childhood ALL and 25% of adult ALL. Composing of putative enhancers in close genomic proximity, super enhancer (SE) is critical for cell identity and the pathogenesis of multiple cancers. Belonging to the cytosolute linker protein group, FYB1 is essential for TCR signaling and extensively studied in terms of tumor pathogenesis and metastasis. Dissecting the role of FYN binding protein 1 (FYB1) in T-ALL holds the potential to improve the treatment outcome and prognosis of T-ALL.</p><p><strong>Methods: </strong>In this study, SEs were explored using public H3K27ac ChIP-seq data derived from T-ALL cell lines, AML cell lines and hematopoietic stem and progenitor cells (HSPCs). Downstream target of FYB1 gene was identified by RNA-seq. Effects of shRNA-mediated downregulation of FYB1 and immunoglobulin lambda-like polypeptide 1 (IGLL1) on self-renewal of T-ALL cells were evaluated <i>in vitro</i> and/or <i>in vivo</i>.</p><p><strong>Results: </strong>As an SE-driven gene, overexpression of FYB1 was observed in T-ALL, according to the Cancer Cell Line Encyclopedia database. <i>In vitro</i>, knocking down FYB1 led to comprised growth and enhanced apoptosis of T-ALL cells. <i>In vivo</i>, downregulation of FYB1 significantly decreased the disease burden by suppressing tumor growth and improved survival rate. Knocking down FYB1 resulted in significantly decreased expression of IGLL1 that was also an SE-driven gene in T-ALL. As a downstream target of FYB1, IGLL1 exerted similar role as FYB1 in inhibiting growth of T-ALL cells.</p><p><strong>Conclusion: </strong>Our results suggested that FYB1 gene played important role in regulating self-renewal of T-ALL cells by activating IGLL1, representing a promising therapeutic target for T-ALL patients.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2023 ","pages":"3804605"},"PeriodicalIF":4.1,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41147173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dupilumab's Impact on Blood Parameters in Nasal Polyposis: 18-Month Follow-Up in Real Life.","authors":"Antonella Loperfido, Andrea Ciofalo, Carlo Cavaliere, Elona Begvarfaj, Francesca Cascone, Giacomo Alfonzo, Rosalba Cadeddu, Stefano Millarelli, Gianluca Bellocchi, Antonio Greco, Marco de Vincentiis, Simonetta Masieri","doi":"10.1155/2023/4027701","DOIUrl":"10.1155/2023/4027701","url":null,"abstract":"<p><strong>Background: </strong>Dupilumab represents the first approved biological for severe uncontrolled chronic rhinosinusitis with nasal polyps (CRSwNP).</p><p><strong>Objective: </strong>Aim of this paper is to provide a multicentric real-life study about treatment with dupilumab for CRSwNP with a special focus on blood parameters and IgE, IgG, and IgA.</p><p><strong>Method: </strong>A retrospective data collection was jointly conducted at the Otolaryngology departments of San Camillo Forlanini Hospital and University of Rome \"La Sapienza\" from December 2020 to January 2023.</p><p><strong>Results: </strong>A total of 130 patients were included in the study. Monitoring our patients for 18 months, we observed a reduction in nasal polyposis and an improvement in symptoms and their impact on quality of life. Regarding blood tests, a transient increase in blood eosinophils was found in most cases. Total IgE showed a gradual decrease in values. IgG and IgA also showed a slight reduction of values, while remaining within normal ranges.</p><p><strong>Conclusion: </strong>To the best of our knowledge, this is the first study to evaluate the impact of dupilumab on several blood parameters in patients receiving treatment for CRswNP. Further studies are needed to confirm our results and to understand the underlying immunological mechanisms.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2023 ","pages":"4027701"},"PeriodicalIF":3.5,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10516700/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41132761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}