Journal of Immunology Research最新文献

筛选
英文 中文
Influence of PD-1 and PD-1L Immune Exhaustion Receptors on Immune Reconstruction in People Living With HIV. PD-1和PD-1L免疫衰竭受体对HIV感染者免疫重建的影响
IF 3.6 3区 医学
Journal of Immunology Research Pub Date : 2025-09-30 eCollection Date: 2025-01-01 DOI: 10.1155/jimr/2462382
Bogusz Aksak-Wąs, Karolina Skonieczna-Żydecka, Miłosz Parczewski, Rafał Hrynkiewicz, Filip Lewandowski, Karol Serwin, Kaja Mielczak, Adam Majchrzak, Franciszek Lenkiewicz, Paulina Niedźwiedzka-Rystwej, Poorani Gurumallesh
{"title":"Influence of PD-1 and PD-1L Immune Exhaustion Receptors on Immune Reconstruction in People Living With HIV.","authors":"Bogusz Aksak-Wąs, Karolina Skonieczna-Żydecka, Miłosz Parczewski, Rafał Hrynkiewicz, Filip Lewandowski, Karol Serwin, Kaja Mielczak, Adam Majchrzak, Franciszek Lenkiewicz, Paulina Niedźwiedzka-Rystwej, Poorani Gurumallesh","doi":"10.1155/jimr/2462382","DOIUrl":"10.1155/jimr/2462382","url":null,"abstract":"<p><strong>Introduction: </strong>The progressive immunological impairment associated with human immunodeficiency virus (HIV) infection is partially mediated by the programmed cell death protein-1 (PD-1)/programed death-ligand 1(PD-L1) inhibitory pathway. This investigation aims to evaluate the influence of PD-1 on immune reconstitution in patients undergoing antiretroviral therapy (ART), with data visualized through principal component analysis (PCA).</p><p><strong>Materials and methods: </strong>Data from 52 ART-treated individuals achieving viral suppression were analyzed over 12 months. CD4+, CD8+, CD19+, and PD-1/PD-L1 expressions were quantified via flow cytometry at baseline and after 12 months, and immune recovery was assessed at CD4+ thresholds of 500 and 800/μL and CD4+/CD8+ ratios of >0.8 and >1.0 using linear and logistic regression. PCA was applied to visualize clustering of immune recovery patterns based on PD-1/PD-L1 expression levels and immune cell counts, with statistical significance evaluated using ANOVA.</p><p><strong>Results: </strong>The analyzed group of 52 patients was predominantly male (65.4%; <i>n</i> = 34). PD-1/PD-L1 expression showed modest associations with immune recovery. Higher PD-L1 expression on CD3+ T-cells at baseline was associated with a reduced likelihood of recovery to CD4+>500/μL (OR: 0.79; 95%CI: 0.62-0.99; <i>p</i> = 0.04). Linear regression demonstrated that increased PD-L1 on CD4+ T-cells and PD-1 on CD19+ B-cells positively correlated with higher CD4+/CD8+ ratios at follow-up (coefficient: 0.035 and 0.03, respectively; <i>p</i> < 0.02), while logistic regression indicated that higher PD-1 on CD3+ T-cells increased the odds of recovery to CD4+>500/μL (OR: 1.03; 95% CI: 1.0036-1.07); = 0.03). Notably, this weak signal may result from a general increase in the number of lymphocytes during therapy. PCA did not reveal significant clustering of immune recovery patterns.</p><p><strong>Conclusion: </strong>PD-1 and PD-L1 expressions on immune cells are weakly associated with immune recovery metrics in individuals undergoing ART. Further research is needed to explore their role in immune reconstitution and potential clinical applications.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2025 ","pages":"2462382"},"PeriodicalIF":3.6,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12481824/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification and Validation of Fibroblast-Associated Genes in Osteoarthritis Based on High-Dimensional Weighted Gene Coexpression Network Analysis. 基于高维加权基因共表达网络分析的骨关节炎成纤维细胞相关基因鉴定与验证。
IF 3.6 3区 医学
Journal of Immunology Research Pub Date : 2025-09-28 eCollection Date: 2025-01-01 DOI: 10.1155/jimr/5547701
Juan Xiao, Wei Lei, Hao Zhang, Feng Niu, Qunhai Wu, Honglin Pi, Poorani Gurumallesh
{"title":"Identification and Validation of Fibroblast-Associated Genes in Osteoarthritis Based on High-Dimensional Weighted Gene Coexpression Network Analysis.","authors":"Juan Xiao, Wei Lei, Hao Zhang, Feng Niu, Qunhai Wu, Honglin Pi, Poorani Gurumallesh","doi":"10.1155/jimr/5547701","DOIUrl":"10.1155/jimr/5547701","url":null,"abstract":"<p><p><b>Background:</b> Osteoarthritis (OA) is a degenerative joint disease with articular cartilage destruction, triggering a pro-inflammatory response. The aim of this study was to screen key genes associated with fibroblasts based on single-cell transcriptomic data and explore their potential value in OA diagnosis. <b>Methods:</b> We obtained RNA sequencing (RNA-seq) and single-cell RNA-seq (scRNA-seq) data of OA from the Gene Expression Omnibus (GEO) database. The CellChat package for cell-to-cell communication analysis and identification of possible ligand-receptor pairs. High-dimensional weighted gene coexpression network analysis (hdWGCNA) was applied to identify the gene modules, and the key genes in the modules were identified and subjected to functional enrichment analysis. Subsequently, limma packages were used to screen for differentially expressed genes (DEGs) between OA and its control samples. Finally, the R package multipleROC was used to test the diagnostic potential of the screened key genes and to construct an OA diagnostic model using the rms package. <b>Result:</b> Eight cell populations were identified and annotated based on scRNA-seq and the percentage of fibroblasts was the highest. The cell-cell communication analysis has suggested that the highest communication probability was seen between mesenchymal cells/T cells and fibroblasts through the pairs of CD99-CD99. The hdWGCNA analysis suggested that genes of modules M3, M4, M5, M6, and M8 (50 genes in total) were highly expressed in fibroblasts. Thereafter, we obtained 394 DEGs in OA and its control samples and took intersections with 50 modular genes and identified seven central genes (including apolipoprotein D <i>[APOD]</i>, biglycan <i>[BGN]</i>, <i>MXRA5</i>, <i>THY1</i>, <i>C1QTNF3</i>, dermatopontin [<i>DPT]</i>, and osteoglycin <i>[OGN]</i>). The constructed diagnostic models showed good predictive performance with all area under the curve (AUC) values >0.8. Finally, a satisfactory diagnostic model was established using these seven genes, and the differences in mRNA expression levels of these genes in OA and normal tissues were verified. <b>Conclusion:</b> For the first time, our study systematically screened and validated key genes with diagnostic potential based on fibroblast-specific single-cell data in combination with hdWGCNA, providing a new theoretical basis and research direction for molecular typing and diagnosis of OA.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2025 ","pages":"5547701"},"PeriodicalIF":3.6,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12477407/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Dynamic Expression Changes of Neutrophil Extracellular Traps in Mouse Apical Periodontitis: A Potential Correlation With IL-17. 小鼠根尖牙周炎中性粒细胞胞外陷阱的动态表达变化:与IL-17的潜在相关性。
IF 3.6 3区 医学
Journal of Immunology Research Pub Date : 2025-09-15 eCollection Date: 2025-01-01 DOI: 10.1155/jimr/8039031
Zihan Ma, Xiaoyue Sun, Ying Lin, Zijun Wang, Qing Nie, Jingjing Yu, Jingwen Yang, Lingxin Zhu
{"title":"The Dynamic Expression Changes of Neutrophil Extracellular Traps in Mouse Apical Periodontitis: A Potential Correlation With IL-17.","authors":"Zihan Ma, Xiaoyue Sun, Ying Lin, Zijun Wang, Qing Nie, Jingjing Yu, Jingwen Yang, Lingxin Zhu","doi":"10.1155/jimr/8039031","DOIUrl":"10.1155/jimr/8039031","url":null,"abstract":"<p><p>Neutrophil extracellular traps (NETs) consist of decondensed chromatin and antimicrobial proteins, which are released from neutrophils and have been implicated in several inflammatory diseases. NETs and interleukin (IL)-17 constitute a feed-forward loop that promotes immunopathological development of inflammation. Therefore, this study aimed to investigate the dynamic distribution of NETs and their colocalization with IL-17 during the progression of apical periodontitis in established mouse models. Apical periodontitis was induced in mice by exposing the pulp of the mandibular first molars, with mandibles harvested on day 0, 7, 14, 21, and 28 after pulp exposure. Micro-CT and high-resolution X-ray scanning showed progressive increases in both the area and volume of periapical lesions from day 0 to day 28. Osteoclasts in the periapical lesions were identified using tartrate-resistant acid phosphatase (TRAP) staining, with their numbers peaking on day 21. Immunofluorescence staining was performed for citrullinated histone H3 (CitH3), myeloperoxidase (MPO), neutrophil elastase (NE), and IL-17 to localize NETs and their colocalization with IL-17 in lesions. NETs, which were visible on day 7 and increased gradually until day 21, primarily located in inflammatory infiltration areas of the periapical tissue. Additionally, western blot analysis showed increased CitH3 expression in periapical lesions on day 21 after pulp exposure, further confirming the presence of NETs. Both RT-qPCR and enzyme-linked immunosorbent assay (ELISA) revealed increased IL-17 expression in periapical lesions. The colocalization of CitH3, the major component of NETs and IL-17 peaked on day 21 after pulp exposure. Furthermore, in vitro experiments demonstrated that IL-17 promoted NETs formation under lipopolysaccharide (LPS)-simulated inflammatory conditions. Our findings indicated that NETs expression changed dynamically and suggested a feedback loop between NETs and IL-17 during the development of apical periodontitis in mouse models.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2025 ","pages":"8039031"},"PeriodicalIF":3.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12445725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Astaxanthin Mitigates Dextran Sulfate Sodium-Induced Colitis in Mice by Repairing the Intestinal Barrier, Regulating Specific Intestinal Flora, and Reducing Inflammatory Cell Infiltration. 虾青素通过修复肠道屏障、调节特定肠道菌群和减少炎症细胞浸润来减轻葡聚糖硫酸钠诱导的小鼠结肠炎。
IF 3.6 3区 医学
Journal of Immunology Research Pub Date : 2025-08-21 eCollection Date: 2025-01-01 DOI: 10.1155/jimr/3381950
Guangzhe Shen, Du Yu, Longfei Xu, Tiezheng Yang
{"title":"Astaxanthin Mitigates Dextran Sulfate Sodium-Induced Colitis in Mice by Repairing the Intestinal Barrier, Regulating Specific Intestinal Flora, and Reducing Inflammatory Cell Infiltration.","authors":"Guangzhe Shen, Du Yu, Longfei Xu, Tiezheng Yang","doi":"10.1155/jimr/3381950","DOIUrl":"10.1155/jimr/3381950","url":null,"abstract":"<p><p>As a dietary supplement for humans and animals, Astaxanthin (Ax) is widely believed to possess antioxidant and anti-inflammatory properties. In this study, we attempted to evaluate the protective effects of Ax on dextran sodium sulfate (DSS)-induced colitis in mice and the underlying molecular mechanism. Our results suggested that Ax significantly reduced the severity of DSS-induced colitis in mice, as evidenced by increased colon length, decreased disease activity index (DAI), and attenuated inflammatory factors. In addition, Ax significantly increased the diversity of gut microbiota in mice with colitis, remodeled the microbial composition, promoted the production of beneficial bacteria (e.g., Lactobacillaceae), and inhibited the production of harmful bacteria (e.g., Lachnospiraceae and Muribaculaceae). In conclusion, Ax alleviated DSS-induced colitis by maintaining the intestinal barrier and regulating intestinal microbes.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2025 ","pages":"3381950"},"PeriodicalIF":3.6,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12393947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144956967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Corrigendum to "miR-214-3p Deficiency Enhances Caspase-1-Dependent Pyroptosis of Microglia in White Matter Injury". “miR-214-3p缺陷增强白质损伤中caspase -1依赖性小胶质细胞焦亡”的更正。
IF 3.6 3区 医学
Journal of Immunology Research Pub Date : 2025-08-13 eCollection Date: 2025-01-01 DOI: 10.1155/jimr/9826132
{"title":"Corrigendum to \"miR-214-3p Deficiency Enhances Caspase-1-Dependent Pyroptosis of Microglia in White Matter Injury\".","authors":"","doi":"10.1155/jimr/9826132","DOIUrl":"https://doi.org/10.1155/jimr/9826132","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1155/2022/1642896.].</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2025 ","pages":"9826132"},"PeriodicalIF":3.6,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367391/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144956905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Intradermally Administered Retinoic Acid or Vitamin D3-Loaded Liposomes Induce Tolerogenic Skin Dendritic Cells. 皮内给予维甲酸或维生素d3负载脂质体诱导耐受性皮肤树突状细胞。
IF 3.6 3区 医学
Journal of Immunology Research Pub Date : 2025-08-04 eCollection Date: 2025-01-01 DOI: 10.1155/jimr/2208155
Noémi Anna Nagy, Sanne G Celant, Toni M M van Capel, Rinske Sparrius, Fernando Lozano Vigario, Ronald van Ree, Bram Slütter, Teunis B H Geijtenbeek, Sander W Tas, Esther C de Jong
{"title":"Intradermally Administered Retinoic Acid or Vitamin D3-Loaded Liposomes Induce Tolerogenic Skin Dendritic Cells.","authors":"Noémi Anna Nagy, Sanne G Celant, Toni M M van Capel, Rinske Sparrius, Fernando Lozano Vigario, Ronald van Ree, Bram Slütter, Teunis B H Geijtenbeek, Sander W Tas, Esther C de Jong","doi":"10.1155/jimr/2208155","DOIUrl":"10.1155/jimr/2208155","url":null,"abstract":"<p><p>In vivo targeting of dendritic cells (DCs) with nanocarriers containing tolerogenic adjuvants is an attractive strategy to dampen inflammation. Here, we used ex vivo skin vaccination to examine the effect of intradermal injection of liposomes loaded with the tolerogenic adjuvants all-trans retinoic acid (RA) and vitamin D3 (VD3). We investigated the effect of intradermal liposome injection on skin DCs and the skin DC-induced T cell response. Our study shows that intradermal injection of RA or VD3-loaded anionic phospholipid 1,2-distearoyl-sn-glycero-3-phosphoglycerol (DSPG) liposomes selectively induces CD14+ dermal DC (DDC) migration while reducing migration of CD1a dim DDCs. Migrated CD14+ DDCs displayed a partially immature phenotype. RA or VD3 liposome-treated CD1a dim DDCs exhibited reduced expression of maturation markers and induced expression of coinhibitory immunoglobulin-like transcript 3 (ILT3). VD3 liposome-treated CD14+ DDCs, as well as, CD1a dim DDCs, exhibited reduced expression of maturation markers, induction of coinhibitory molecules ILT3, and programmed death-ligand 1 (PD-L1). Migrated DCs from RA or VD3 liposome-injected skin differentiated naïve CD4+ T cells into FoxP3+ CD127 low and ICOS+ Tregs, expressing functional regulatory markers. Thus, our findings provide further substantiation for in vivo DC-modulating vaccines with tolerogenic liposomes as a putative clinical therapy for autoimmune diseases and allergies.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2025 ","pages":"2208155"},"PeriodicalIF":3.6,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12339165/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144821529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Corrigendum to "Impaired Proliferation of CD8+ T Cells Stimulated with Monocyte-Derived Dendritic Cells Previously Matured with Thapsigargin-Stimulated LAD2 Human Mast Cells". “单核细胞来源的树突状细胞刺激CD8+ T细胞增殖受损,先前由thapsigarin刺激的LAD2人肥大细胞成熟”的更正。
IF 3.6 3区 医学
Journal of Immunology Research Pub Date : 2025-07-30 eCollection Date: 2025-01-01 DOI: 10.1155/jimr/9807058
{"title":"Corrigendum to \"Impaired Proliferation of CD8<sup>+</sup> T Cells Stimulated with Monocyte-Derived Dendritic Cells Previously Matured with Thapsigargin-Stimulated LAD2 Human Mast Cells\".","authors":"","doi":"10.1155/jimr/9807058","DOIUrl":"https://doi.org/10.1155/jimr/9807058","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1155/2024/5537948.].</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2025 ","pages":"9807058"},"PeriodicalIF":3.6,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12328042/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Single-Cell Data and Weighted Correlation Network Analysis Revealed the Regulatory Mechanisms of Macrophages in Carotid Plaques. 单细胞数据和加权相关网络分析揭示了巨噬细胞在颈动脉斑块中的调节机制。
IF 3.6 3区 医学
Journal of Immunology Research Pub Date : 2025-07-21 eCollection Date: 2025-01-01 DOI: 10.1155/jimr/9987367
Yakun Ding, Xiaoyang Niu, Peng Guo, Bing Wang
{"title":"Single-Cell Data and Weighted Correlation Network Analysis Revealed the Regulatory Mechanisms of Macrophages in Carotid Plaques.","authors":"Yakun Ding, Xiaoyang Niu, Peng Guo, Bing Wang","doi":"10.1155/jimr/9987367","DOIUrl":"10.1155/jimr/9987367","url":null,"abstract":"<p><p><b>Background:</b> Macrophages play a critical role in carotid plaque. Understanding the mechanisms of carotid plaque formation based on macrophage heterogeneity could provide valuable insights for clinical intervention. <b>Methods:</b> Single-cell transcriptome and bulk RNA-seq data of carotid plaque were obtained from public databases. Weighted gene correlation network analysis (WGCNA) identified gene modules linked to unstable plaques. Macrophage marker genes were intersected with module genes of WGCNA, followed by using randomForest and LASSO regression to pinpoint key genes. Quantitative real-time PCR (qRT-PCR) and Western blot were used to verify the regulation of key genes at the cellular level. The correlation between the key genes and inflammatory phenotypes was examined by single-sample gene set enrichment analysis (ssGSEA). <b>Results:</b> Single-cell clustering revealed major cellular subpopulations, with elevated macrophage infiltration in carotid plaque. Six key macrophage-associated genes (<i>ADPGK, ATP6V1F, CX3CR1, MYO9B, RNF135</i>, and <i>SLC7A8</i>) were discovered. The qRT-PCR results demonstrated upregulation of <i>ADPGK, ATP6V1F</i>, and <i>RNF135</i> genes in vascular smooth muscle cells (VSMCs) treated with oxidized low-density lipoprotein (ox-LDL), except for <i>CX3CR1</i>, which was downregulated. Protein expression results showed that expressions of <i>ADPGK, ATP6V1F, RNF135</i>, and <i>SLC7A8</i> were significantly elevated in the ox-LDL-VSMC group. In addition, most of the immune cells showed significant differences between the unstable arterial plaque group and the control group. <b>Conclusion:</b> This study discovered potential biomarkers that affected carotid plaque progression and macrophage regulation at the single-cell level, and examined their regulatory roles in immune regulation, programed cell death (PCD), and inflammatory factor modulation.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2025 ","pages":"9987367"},"PeriodicalIF":3.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12303652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Epigenetic Regulation of B Cell Memory Formation: A Poised Model for B Cell Epigenetic Reprograming. B细胞记忆形成的表观遗传调控:B细胞表观遗传重编程的平衡模型。
IF 3.6 3区 医学
Journal of Immunology Research Pub Date : 2025-07-17 eCollection Date: 2025-01-01 DOI: 10.1155/jimr/9328523
Carlos Valverde-Hernandez
{"title":"Epigenetic Regulation of B Cell Memory Formation: A Poised Model for B Cell Epigenetic Reprograming.","authors":"Carlos Valverde-Hernandez","doi":"10.1155/jimr/9328523","DOIUrl":"10.1155/jimr/9328523","url":null,"abstract":"<p><p>The formation of B cell immunological memory happens after the first encounter with a pathogen. At the germinal center (GC), B cells experience complex transcriptional and epigenetic transitions to differentiate into memory B cells (MBCs) and plasma cells (PCs). In particular, the differentiation of GC B cells into MBCs has been poorly understood, and no clear conclusions on the signals and transcription factors leading to this cell fate have been identified. Recent discoveries in epigenetics and immune memory have elucidated the essential role of epigenetic regulators in establishing the memory B cell (MBC) fate. DNA methylation regulators, histone modifiers, noncoding RNAs (ncRNAs), and chromatin remodelers orchestrate a dynamic reprograming of the MBC phenotype. Positive and negative epigenetic regulators of the B cell program collaborate at each differentiation stage and allow for complex chromatin topology rearrangements and dynamic exposure to transcription and translation. Following MBC fate determination at the GC, the acquired epigenetic modifications induce a poised regulatory state where genes are epigenetically marked to remain transcriptionally inactive, but primed for rapid activation upon stimuli. Thus, a poised epigenetic control over gene expression governs MBC formation and a novel model of epigenetic reprograming is proposed. This model provides a novel perspective on how the B cell fate is determined in the GC and memory is formed, offering insights for improved vaccination and therapeutical approaches.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2025 ","pages":"9328523"},"PeriodicalIF":3.6,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12289371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144707761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Integrated Transcriptomic Analysis Provided Diagnostic and Pathophysiological Insights for Epilepsy. 综合转录组学分析为癫痫的诊断和病理生理学提供了见解。
IF 3.5 3区 医学
Journal of Immunology Research Pub Date : 2025-07-15 eCollection Date: 2025-01-01 DOI: 10.1155/jimr/5925485
Shuang Li, Zhigang Wang, Yake Zheng, Yunqing Ma, Zhi Huang, Yajun Lian
{"title":"Integrated Transcriptomic Analysis Provided Diagnostic and Pathophysiological Insights for Epilepsy.","authors":"Shuang Li, Zhigang Wang, Yake Zheng, Yunqing Ma, Zhi Huang, Yajun Lian","doi":"10.1155/jimr/5925485","DOIUrl":"10.1155/jimr/5925485","url":null,"abstract":"<p><p><b>Background:</b> Epilepsy is a common neurological disorder involving multiple genes and molecular pathways. Study of differentially expressed genes (DEGs) and hub genes related to epilepsy can help reveal the pathophysiologic basis and improve potential diagnostic and therapeutic strategies. <b>Methods:</b> Transcriptome data of two epilepsy datasets (GSE143272 and GSE32534) and single-cell sequencing data (GSE201048) were collected from the Gene Expression Omnibus (GEO) database. Differential expression analysis was performed using Limma R package, and the hub genes were identified and analyzed utilizing STRING database and Cytoscape software. The clusterProfiler R package was used to perform gene function enrichment analysis and an epilepsy diagnostic model was constructed with the hub genes. The model performance was assessed according to receiver operating characteristic (ROC) curves. <b>Results:</b> Multiple DEGs linked to epilepsy were identified and 20 common DEGs between the two datasets were revealed. Eleven hub genes closely associated with epilepsy were identified by protein-protein interaction (PPI) network analysis. CD3D, CD3G, CTSW, and JCHAIN were consistently expressed in the GSE143272 and GSE32534 datasets and all showed a low expression in epilepsy samples. In particular, the diagnostic model developed with the four genes demonstrated a strong discriminatory ability in both datasets (all area under curve (AUC) > 0.7). Functional enrichment and single-cell analysis revealed that these key genes were closely related to T cell function, suggesting that they may play an important role in the immune regulation of epilepsy. <b>Conclusion:</b> This study successfully identified four key genes linked to epilepsy, contributing to the molecular diagnosis of epilepsy.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2025 ","pages":"5925485"},"PeriodicalIF":3.5,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信