The Dynamic Expression Changes of Neutrophil Extracellular Traps in Mouse Apical Periodontitis: A Potential Correlation With IL-17.

IF 3.6 3区 医学 Q2 IMMUNOLOGY
Journal of Immunology Research Pub Date : 2025-09-15 eCollection Date: 2025-01-01 DOI:10.1155/jimr/8039031
Zihan Ma, Xiaoyue Sun, Ying Lin, Zijun Wang, Qing Nie, Jingjing Yu, Jingwen Yang, Lingxin Zhu
{"title":"The Dynamic Expression Changes of Neutrophil Extracellular Traps in Mouse Apical Periodontitis: A Potential Correlation With IL-17.","authors":"Zihan Ma, Xiaoyue Sun, Ying Lin, Zijun Wang, Qing Nie, Jingjing Yu, Jingwen Yang, Lingxin Zhu","doi":"10.1155/jimr/8039031","DOIUrl":null,"url":null,"abstract":"<p><p>Neutrophil extracellular traps (NETs) consist of decondensed chromatin and antimicrobial proteins, which are released from neutrophils and have been implicated in several inflammatory diseases. NETs and interleukin (IL)-17 constitute a feed-forward loop that promotes immunopathological development of inflammation. Therefore, this study aimed to investigate the dynamic distribution of NETs and their colocalization with IL-17 during the progression of apical periodontitis in established mouse models. Apical periodontitis was induced in mice by exposing the pulp of the mandibular first molars, with mandibles harvested on day 0, 7, 14, 21, and 28 after pulp exposure. Micro-CT and high-resolution X-ray scanning showed progressive increases in both the area and volume of periapical lesions from day 0 to day 28. Osteoclasts in the periapical lesions were identified using tartrate-resistant acid phosphatase (TRAP) staining, with their numbers peaking on day 21. Immunofluorescence staining was performed for citrullinated histone H3 (CitH3), myeloperoxidase (MPO), neutrophil elastase (NE), and IL-17 to localize NETs and their colocalization with IL-17 in lesions. NETs, which were visible on day 7 and increased gradually until day 21, primarily located in inflammatory infiltration areas of the periapical tissue. Additionally, western blot analysis showed increased CitH3 expression in periapical lesions on day 21 after pulp exposure, further confirming the presence of NETs. Both RT-qPCR and enzyme-linked immunosorbent assay (ELISA) revealed increased IL-17 expression in periapical lesions. The colocalization of CitH3, the major component of NETs and IL-17 peaked on day 21 after pulp exposure. Furthermore, in vitro experiments demonstrated that IL-17 promoted NETs formation under lipopolysaccharide (LPS)-simulated inflammatory conditions. Our findings indicated that NETs expression changed dynamically and suggested a feedback loop between NETs and IL-17 during the development of apical periodontitis in mouse models.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2025 ","pages":"8039031"},"PeriodicalIF":3.6000,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12445725/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Immunology Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1155/jimr/8039031","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Neutrophil extracellular traps (NETs) consist of decondensed chromatin and antimicrobial proteins, which are released from neutrophils and have been implicated in several inflammatory diseases. NETs and interleukin (IL)-17 constitute a feed-forward loop that promotes immunopathological development of inflammation. Therefore, this study aimed to investigate the dynamic distribution of NETs and their colocalization with IL-17 during the progression of apical periodontitis in established mouse models. Apical periodontitis was induced in mice by exposing the pulp of the mandibular first molars, with mandibles harvested on day 0, 7, 14, 21, and 28 after pulp exposure. Micro-CT and high-resolution X-ray scanning showed progressive increases in both the area and volume of periapical lesions from day 0 to day 28. Osteoclasts in the periapical lesions were identified using tartrate-resistant acid phosphatase (TRAP) staining, with their numbers peaking on day 21. Immunofluorescence staining was performed for citrullinated histone H3 (CitH3), myeloperoxidase (MPO), neutrophil elastase (NE), and IL-17 to localize NETs and their colocalization with IL-17 in lesions. NETs, which were visible on day 7 and increased gradually until day 21, primarily located in inflammatory infiltration areas of the periapical tissue. Additionally, western blot analysis showed increased CitH3 expression in periapical lesions on day 21 after pulp exposure, further confirming the presence of NETs. Both RT-qPCR and enzyme-linked immunosorbent assay (ELISA) revealed increased IL-17 expression in periapical lesions. The colocalization of CitH3, the major component of NETs and IL-17 peaked on day 21 after pulp exposure. Furthermore, in vitro experiments demonstrated that IL-17 promoted NETs formation under lipopolysaccharide (LPS)-simulated inflammatory conditions. Our findings indicated that NETs expression changed dynamically and suggested a feedback loop between NETs and IL-17 during the development of apical periodontitis in mouse models.

小鼠根尖牙周炎中性粒细胞胞外陷阱的动态表达变化:与IL-17的潜在相关性。
中性粒细胞胞外陷阱(NETs)由去致密的染色质和抗菌蛋白组成,它们从中性粒细胞中释放出来,并与几种炎症性疾病有关。NETs和白细胞介素(IL)-17构成一个前馈回路,促进炎症的免疫病理发展。因此,本研究旨在探讨NETs在建立的小鼠根尖牙周炎发展过程中的动态分布及其与IL-17的共定位。暴露小鼠下颌第一磨牙牙髓诱导根尖牙周炎,分别于牙髓暴露后第0、7、14、21、28天取龋。显微ct和高分辨率x线扫描显示,从第0天到第28天,根尖周围病变的面积和体积都在逐渐增加。采用抗酒石酸酸性磷酸酶(TRAP)染色检测根尖周围病变中的破骨细胞,破骨细胞数量在第21天达到峰值。对瓜氨酸组蛋白H3 (CitH3)、髓过氧化物酶(MPO)、中性粒细胞弹性酶(NE)和IL-17进行免疫荧光染色,定位NETs及其与IL-17在病变中的共定位。NETs在第7天可见,直到第21天逐渐增加,主要位于根尖周组织的炎症浸润区。此外,western blot分析显示,牙髓暴露后第21天,尖周病变中CitH3的表达增加,进一步证实了NETs的存在。RT-qPCR和酶联免疫吸附试验(ELISA)均显示IL-17在根尖周围病变中的表达升高。牙髓暴露后第21天,CitH3、NETs的主要成分和IL-17的共定位达到高峰。此外,体外实验表明,在脂多糖(LPS)模拟的炎症条件下,IL-17促进NETs的形成。我们的研究结果表明,在小鼠根尖牙周炎模型的发展过程中,NETs的表达是动态变化的,并且表明NETs和IL-17之间存在反馈回路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
6.90
自引率
2.40%
发文量
423
审稿时长
15 weeks
期刊介绍: Journal of Immunology Research is a peer-reviewed, Open Access journal that provides a platform for scientists and clinicians working in different areas of immunology and therapy. The journal publishes research articles, review articles, as well as clinical studies related to classical immunology, molecular immunology, clinical immunology, cancer immunology, transplantation immunology, immune pathology, immunodeficiency, autoimmune diseases, immune disorders, and immunotherapy.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信