Journal of Forensic Medicine最新文献

{"title":"[Analysis of injury mechanism of vertical compression ankle fracture： Two case reports].","authors":"晴 夏, 捷 敏 陈","doi":"10.12116/j.issn.1004-5619.2021.211005","DOIUrl":"10.12116/j.issn.1004-5619.2021.211005","url":null,"abstract":"吴某，男，41岁，某年8月8日因“被人打伤致右踝关节及全身多处疼痛伴活动受限半天”入院。右踝部CT片示：右后踝骨折，右下胫腓联合损伤？临床诊断为右后踝骨折，右下胫腓骨联合损伤。入院后建议手术治疗，吴某拒绝，故暂予以患肢支具外固定及对症治疗等保守治疗。出院时情况：右踝部肿胀明显减轻。出院医嘱：建议继续治疗，支具外固定4~6周，伤后3个月内严禁患肢下地活动等。原鉴定机构根据四肢长骨骨折评定为轻伤二级。现因办案需要，公安机关委托本鉴定机构对吴某右踝关节骨折的成伤机制及损伤程度进行法医学鉴定。.","PeriodicalId":15899,"journal":{"name":"Journal of Forensic Medicine","volume":"39 5","pages":"525-527"},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138434216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection and Analysis of Blood Dexmedetomidine in Drug-Facilitated Cases.","authors":"Wen-Ya Zhai, Li-Na Wu, Shuo Yang, Bao-Hua Shen, Yan Shi","doi":"10.12116/j.issn.1004-5619.2022.320306","DOIUrl":"10.12116/j.issn.1004-5619.2022.320306","url":null,"abstract":"<p><strong>Objectives: </strong>To establish a simple and rapid qualitative and quantitative detection method of dexmedetomidine in blood.</p><p><strong>Methods: </strong>Blood was separated on the Allure PFP Propyl liquid chromatography column with isocratic elution after it was precipitated by acetonitrile and filtered. Qualitative and quantitative analysis of dexmedetomidine was performed using positive ion scan mode and multi-reaction monitoring mode.</p><p><strong>Results: </strong>The limit of detection of dexmedetomidine in blood was 0.2 ng/mL and the limit of quantification was 0.5 ng/mL. The linearity of the method was good in the range of 0.5-1 000 ng/mL, and the correlation coefficient was greater than 0.99. The accuracy of the method was 90.34%-112.67% and the extraction recovery was 50.05%-91.08%, with no significant matrix effect.</p><p><strong>Conclusions: </strong>This method is simple, selective and suitable for the qualitative and quantitative analysis of dexmedetomidine in blood, which can provide a reference for drug-facilitated cases involving dexmedetomidine.</p>","PeriodicalId":15899,"journal":{"name":"Journal of Forensic Medicine","volume":"39 5","pages":"452-456"},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138434230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Polymorphism and mutation investigation of 37 Y-STR loci in Shandong Han population].","authors":"科 建 郭, 磊 黄, 士 林 李, 才 湧 殷, 真 汤","doi":"10.12116/j.issn.1004-5619.2022.520903","DOIUrl":"10.12116/j.issn.1004-5619.2022.520903","url":null,"abstract":"Y染色体具有男性特有、父系遗传的特点，相比常染色体遗传标记有其独特的优点。Y-STR具有非重组、单倍型父系遗传的特征，近年被广泛应用于家系排查、混合斑男性成分检测检验中，已成为公安机关侦破案件的重要手段之一[ 1]。Y染色体分为拟常染色体区和非重组区，拟常染色区常与X染色体发生重组，非重组区不发生重组，法医Y-STR基因座的挑选多分布于非重组区，呈单倍型遗传，且多含有重复序列，在遗传过程中，极易发生突变，突变率约为4×10-3[ 1- 3]。本研究通过采集山东省16个地级市1 490个家系祖孙三代人的样本，进行遗传多态性和突变率分析，以发现家系内部的遗传突变规律，为山东省Y-STR数据的应用比对和国产试剂盒的研发提供遗传学基础数据。.","PeriodicalId":15899,"journal":{"name":"Journal of Forensic Medicine","volume":"39 5","pages":"501-506"},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138434226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Death caused by high-dose difenidol hydrochloride combined with ethanol and potassium chloride poisoning： A case report].","authors":"日 红 高, 新 宙 王, 冰 王, 立 新 郑","doi":"10.12116/j.issn.1004-5619.2022.420604","DOIUrl":"10.12116/j.issn.1004-5619.2022.420604","url":null,"abstract":"陈某，男，27岁，因工作、感情、生活等压力厌世。某日陈某独自骑摩托车旅行，于3月15日入住某民宿，并先后于实体药店和网上平台购买药物。3月22日民宿到期，房东联系不上陈某，敲门不应，遂报警。民警上门查探时发现房门为反锁状态，强行破门后发现陈某已死亡。为明确死亡原因，对其进行法医学鉴定。.","PeriodicalId":15899,"journal":{"name":"Journal of Forensic Medicine","volume":"39 5","pages":"512-515"},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138434219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Identification of testicular atrophy caused by perineal trauma： A case report].","authors":"安 全 马, 晓 晨 马, 俊 杰 方","doi":"10.12116/j.issn.1004-5619.2022.220214","DOIUrl":"10.12116/j.issn.1004-5619.2022.220214","url":null,"abstract":"王某，男，42岁，某年1月2日，因“被他人用刀刺伤右侧阴囊部”入院。出院后感觉双侧睾丸不等大，经医院多次检查后确认右侧睾丸较左侧偏小，怀疑与被刀刺伤有关，故对其损伤程度进行法医学鉴定。.","PeriodicalId":15899,"journal":{"name":"Journal of Forensic Medicine","volume":"39 5","pages":"528-530"},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138434224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Process of Forensic Medicine in DNA Identification of Aged Human Remains.","authors":"Lu Chen, Zhe Zhou, Sheng-Qi Wang","doi":"10.12116/j.issn.1004-5619.2021.511209","DOIUrl":"10.12116/j.issn.1004-5619.2021.511209","url":null,"abstract":"<p><p>Skeleton and teeth are important biological samples. Due to their special structure and strong ability to resist degradation, they are ideal biological materials to retain DNA under natural condition. In many cases, such as historical figure identification, aged skeleton and teeth are usually the only biological samples. However, their DNA is in a state of trace, damage and degradation to different degrees, which requires special experimental treatment to achieve identification. This paper reviews the sample selection, DNA extraction, DNA enrichment and analysis approaches based on relevant research reports in recent years, aiming to promote the further development and improvement of the aged skeleton and teeth identification system.</p>","PeriodicalId":15899,"journal":{"name":"Journal of Forensic Medicine","volume":"39 5","pages":"478-486"},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138434231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Dead hair detected from <i>Gelsemium elegans</i> poisoning： A case report].","authors":"建 波 陈, 影 郭, 再 勇 陈, 人 辉 鲍, 繁 荣 孔","doi":"10.12116/j.issn.1004-5619.2022.420903","DOIUrl":"10.12116/j.issn.1004-5619.2022.420903","url":null,"abstract":"王某，女，74岁，某年9月16日，购买中草药“红木香”熬成汤药后与其丈夫陈某一起服用。约10 min后王某出现头晕、昏迷，经送医院治疗后一直处于植物生存状态，直至次年5月13日死亡。陈某在送院途中发生抽搐，经治疗后好转并出院。.","PeriodicalId":15899,"journal":{"name":"Journal of Forensic Medicine","volume":"39 5","pages":"509-511"},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138434218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of an Analysis Model of mRNA Markers in Menstrual Blood Based on Naïve Bayes and Multivariate Logistic Regression Methods.","authors":"Qi Zhang, He-Miao Zhao, Kang Yang, Jing Chen, Rui-Qin Yang, Chong Wang","doi":"10.12116/j.issn.1004-5619.2021.511207","DOIUrl":"10.12116/j.issn.1004-5619.2021.511207","url":null,"abstract":"<p><strong>Objectives: </strong>To establish the menstrual blood identification model based on Naïve Bayes and multivariate logistic regression methods by using specific mRNA markers in menstrual blood detection technology combined with statistical methods, and to quantitatively distinguish menstrual blood from other body fluids.</p><p><strong>Methods: </strong>Body fluids including 86 menstrual blood, 48 peripheral blood, 48 vaginal secretions, 24 semen and 24 saliva samples were collected. RNA of the samples was extracted and cDNA was obtained by reverse transcription. Five menstrual blood-specific markers including members of the matrix metalloproteinase (MMP) family MMP3, MMP7, MMP11, progestogens associated endometrial protein (PAEP) and stanniocalcin-1 (STC1) were amplified and analyzed by electrophoresis. The results were analyzed by Naïve Bayes and multivariate logistic regression.</p><p><strong>Results: </strong>The accuracy of the classification model constructed was 88.37% by Naïve Bayes and 91.86% by multivariate logistic regression. In non-menstrual blood samples, the distinguishing accuracy of peripheral blood, saliva and semen was generally higher than 90%, while the distinguishing accuracy of vaginal secretions was lower, which were 16.67% and 33.33%, respectively.</p><p><strong>Conclusions: </strong>The mRNA detection technology combined with statistical methods can be used to establish a classification and discrimination model for menstrual blood, which can distignuish the menstrual blood and other body fluids, and quantitative description of analysis results, which has a certain application value in body fluid stain identification.</p>","PeriodicalId":15899,"journal":{"name":"Journal of Forensic Medicine","volume":"39 5","pages":"447-451"},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138434228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Disseminated mucormycosis leading to death： A case report].","authors":"钳 钳 马, 云 张, 丽 娜 秦","doi":"10.12116/j.issn.1004-5619.2020.401005","DOIUrl":"10.12116/j.issn.1004-5619.2020.401005","url":null,"abstract":"某男，68岁，于某年9月11日因“上腹疼痛1周”入住某医院治疗。10月30日行“胆囊切除+胆总管切开胆道镜探查+T管引流术”，当天下午开始出现血压低，转重症监护室继续治疗。该男病情仍持续恶化，逐渐出现少尿、神志昏迷症状。血常规检查提示红细胞、血红蛋白及血小板持续减少。11月13日20：55突然出现心率减慢，血压下降，经抢救无效，于当日22：45宣告临床死亡。医院死亡诊断：急性上消化道大出血，失血性休克；多器官功能障碍综合征；胆源性胰腺炎；胆总管多发结石并急性单纯性胆管炎；胆囊多发结石并胆囊炎；肝脓肿；脑梗死后遗症；陈旧性脑干梗死；血管性痴呆；帕金森综合征；高血压2级；2型糖尿病；慢性胃炎。.","PeriodicalId":15899,"journal":{"name":"Journal of Forensic Medicine","volume":"39 5","pages":"507-509"},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138434221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of Genes Co-Associated with Sudden Infant Death Syndrome and Infectious Sudden Death in Infancy and Bioinformatics Analysis of Their Regulatory Networks.","authors":"Yu-Xin Sun, Xiao-Juan Gong, Xiu-Li Hao, Yu-Xin Tian, Yi-Ming Chen, Bao Zhang, Chun-Xia Yan","doi":"10.12116/j.issn.1004-5619.2022.420803","DOIUrl":"10.12116/j.issn.1004-5619.2022.420803","url":null,"abstract":"<p><strong>Objectives: </strong>The common differentially expressed mRNAs in brain, heart and liver tissues of deceased sudden infant death syndrome (SIDS) and infectious sudden death in infancy (ISDI) confirmed by autopsy was screened by bioinformatics to explore the common molecular markers and pathogenesis of SIDS and ISDI.</p><p><strong>Methods: </strong>The datasets of GSE70422 and GSE136992 were downloaded, the limma of R software was used to screen differentially expressed mRNA in different tissue samples of SIDS and ISDI decedents for overlapping analysis. The clusterProfiler of R software was used to conduct gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. The protein-protein interaction (PPI) network was constructed by STRING database, while the hub gene was screened by cytoHubba plug-in.</p><p><strong>Results: </strong>Compared with the control group, there were 19 significant differentially expressed genes in the tissue samples of SIDS and ISDI decedents, among which 16 in the heart tissue and 3 in the liver tissue, and the astrotactin 1 (<i>ASTN1</i>) gene expression difference in the heart tissue was most significant. The PPI network identified Ras homolog family member A (<i>RHOA</i>), integrin subunit alpha 1 (<i>ITGA1</i>), and H2B clustered histone 5 (<i>H2BC5</i>) were hub genes. The analysis of GO and KEGG showed that differentially expressed genes were enriched in the molecular pathways of actin cytoskeleton regulation, focal adhesion and response to mycophenolic acid.</p><p><strong>Conclusions: </strong><i>ASTN1</i>, <i>RHOA</i> and <i>ITGA1</i> may participate in the development of SIDS and ISDI. The enrichment of differentially expressed genes in immune and inflammatory pathways suggests a common molecular regulatory mechanism between SIDS and ISDI. These findings are expected to provide new biomarkers for molecular anatomy and forensic identification of SIDS and ISDI.</p>","PeriodicalId":15899,"journal":{"name":"Journal of Forensic Medicine","volume":"39 5","pages":"433-440"},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138434249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}