Journal of Forensic Medicine最新文献

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Forensic Pathological Diagnosis of Acute and Old Myocardial Infarction Using Fourier Transform Infrared Spectroscopy. 利用傅立叶变换红外光谱对急性和陈旧性心肌梗死进行法医病理诊断。
Journal of Forensic Medicine Pub Date : 2023-12-25 DOI: 10.12116/j.issn.1004-5619.2023.430317
Tian Tian, Xin-Biao Liao, Fu Zhang, Kai-Fei Deng, Ji Zhang, Ping Huang, Yi-Jiu Chen, Jian-Hua Zhang
{"title":"Forensic Pathological Diagnosis of Acute and Old Myocardial Infarction Using Fourier Transform Infrared Spectroscopy.","authors":"Tian Tian, Xin-Biao Liao, Fu Zhang, Kai-Fei Deng, Ji Zhang, Ping Huang, Yi-Jiu Chen, Jian-Hua Zhang","doi":"10.12116/j.issn.1004-5619.2023.430317","DOIUrl":"10.12116/j.issn.1004-5619.2023.430317","url":null,"abstract":"<p><strong>Objectives: </strong>Fourier transform infrared spectroscopy (FTIR) was used to analyze myocardial infarction tissues at different stages of pathological change to achieve the forensic pathology diagnosis of acute and old myocardial infarction.</p><p><strong>Methods: </strong>FTIR spectra data of early ischemic myocardium, necrotic myocardium, and myocardial fibrous tissue in the left ventricular anterior wall of the sudden death group of atherosclerotic heart disease and the myocardium of the normal control group were collected using hematoxylin-eosin (HE) and immunohistochemistry (IHC) staining as a reference, and the data were analyzed using multivariate statistical analysis.</p><p><strong>Results: </strong>The mean normalized spectra of control myocardium, early ischemic myocardium and necrotic myocardium were relatively similar, but the mean second derivative spectra were significantly different. The peak intensity of secondary structure of proteins in early ischemic myocardium was significantly higher than in other types of myocardium, and the peak intensity of the α-helix in necrotic myocardium was the lowest. The peaks of amide Ⅰ and amide Ⅱ in the mean normalized spectra of myocardial fibrous tissue significantly shifted towards higher wave numbers, the peak intensities of amide Ⅱ and amide Ⅲ were higher than those of other types of myocardium, and the peak intensities at 1 338, 1 284, 1 238 and 1 204 cm<sup>-1</sup> in the mean second derivative spectra were significantly enhanced. Principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) showed that FTIR could distinguish different types of myocardium.</p><p><strong>Conclusions: </strong>FTIR technique has the potential to diagnose acute and old myocardial infarction, and provides a new basis for the analysis of the causes of sudden cardiac death.</p>","PeriodicalId":15899,"journal":{"name":"Journal of Forensic Medicine","volume":"39 6","pages":"535-541"},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139478688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Research Progress of Metabolomics Techniques Combined with Machine Learning Algorithm in Wound Age Estimation. 代谢组学技术与机器学习算法在伤口年龄估计中的结合研究进展。
Journal of Forensic Medicine Pub Date : 2023-12-25 DOI: 10.12116/j.issn.1004-5619.2022.421105
Xing-Yu Ma, Hao Cheng, Zhong-Duo Zhang, Ye-Ming Li, Dong Zhao
{"title":"Research Progress of Metabolomics Techniques Combined with Machine Learning Algorithm in Wound Age Estimation.","authors":"Xing-Yu Ma, Hao Cheng, Zhong-Duo Zhang, Ye-Ming Li, Dong Zhao","doi":"10.12116/j.issn.1004-5619.2022.421105","DOIUrl":"10.12116/j.issn.1004-5619.2022.421105","url":null,"abstract":"<p><p>Wound age estimation is the core content in the practice of forensic medicine. Accurate estimation of wound age is a scientific question that needs to be urgently solved by forensic scientists at home and abroad. Metabolomics techniques can effectively detect endogenous metabolites produced by internal or external stimulating factors and describe the dynamic changes of metabolites <i>in vivo</i>. It has the advantages of strong operability, high detection efficiency and accurate quantitative results. Machine learning algorithm has special advantages in processing high-dimensional data sets, which can effectively mine biological information and truly reflect the physiological, disease or injury state of the body. It is a new technical means for efficiently processing high-throughput big data. This paper reviews the status and advantages of metabolomic techniques combined with machine learning algorithm in the research of wound age estimation, and provides new ideas for this research.</p>","PeriodicalId":15899,"journal":{"name":"Journal of Forensic Medicine","volume":"39 6","pages":"596-600"},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139478694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Forensic Validation and Application Evaluation of IDentifier DNA Typing Kit (Yan-Huang34). IDentifier DNA 分型试剂盒的法医验证和应用评估(Yan-Huang34)。
Journal of Forensic Medicine Pub Date : 2023-12-25 DOI: 10.12116/j.issn.1004-5619.2022.520902
Lin-Lin Gao, Wei Xie, Su-Juan Zhu, Da Li, Qin Wang, Liang Hong, You-Ying Li
{"title":"Forensic Validation and Application Evaluation of IDentifier DNA Typing Kit (Yan-Huang34).","authors":"Lin-Lin Gao, Wei Xie, Su-Juan Zhu, Da Li, Qin Wang, Liang Hong, You-Ying Li","doi":"10.12116/j.issn.1004-5619.2022.520902","DOIUrl":"10.12116/j.issn.1004-5619.2022.520902","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the technical performance of IDentifier DNA typing kit (YanHuang34) and evaluate its forensic application value.</p><p><strong>Methods: </strong>Following the <i>Criterion of Forensic Science Human Fluorescence STR Multiplex Amplification Reagent</i> (GB/T 37226-2018), IDentifier DNA typing kit (YanHuang34) was verified in 11 aspects of species specificity, veracity, sensibility, adaptability, inhibitor tolerance, consistency, balance, reaction condition verification, mixed samples, stability and inter batch consistency. The system efficiency of IDentifier DNA typing kit (YanHuang34) was compared with the PowerPlex<sup>®</sup> Fusion 6C System, VersaPlex<sup>®</sup> 27PY System and VeriFiler<sup>TM</sup> Plus PCR Amplification Kit. The IDentifier DNA typing kit (YanHuang34) was used to detect the swabs of biological samples in daily cases and the STR performances were observed.</p><p><strong>Results: </strong>IDentifier DNA typing kit (YanHuang34) had good species specificity, veracity, adaptability, inhibitor tolerance and balance. The sensibility was up to 0.062 5 ng. It was able to detect different types of samples, degraded samples and inhibitor mixed samples. Complete DNA typing could be obtained for samples with the mixture ratio less than 4∶1. The system efficiency of IDentifier DNA typing kit (YanHuang34) was very high, with TDP up to 1-1.08×10<sup>-37</sup>, CPE<sub>trio</sub> and CPE<sub>duo</sub> up to 1-5.47×10<sup>-14</sup> and 1-6.43×10<sup>-9</sup>, respectively. For the touched biological samples in actual cases, the effective detection rate was 21.05%. The system efficiency of kinship, single parent and full sibling identifications was effectively improved.</p><p><strong>Conclusions: </strong>The IDentifier DNA typing kit (YanHuang34) is adaptive to the GB/T 37226-2018 requirements. It can be used for individual identification and paternity identification, and is suitable for application in the field of forensic science.</p>","PeriodicalId":15899,"journal":{"name":"Journal of Forensic Medicine","volume":"39 6","pages":"579-585"},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139478689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Death due to gastric perforation caused by congenital defect of gastric musculature in a toddler: A case report]. [一名幼儿因胃部肌肉先天缺陷导致胃穿孔死亡:病例报告]。
Journal of Forensic Medicine Pub Date : 2023-12-25 DOI: 10.12116/j.issn.1004-5619.2023.430309
赛 超 陈, 明 昌 张
{"title":"[Death due to gastric perforation caused by congenital defect of gastric musculature in a toddler: A case report].","authors":"赛 超 陈, 明 昌 张","doi":"10.12116/j.issn.1004-5619.2023.430309","DOIUrl":"10.12116/j.issn.1004-5619.2023.430309","url":null,"abstract":"","PeriodicalId":15899,"journal":{"name":"Journal of Forensic Medicine","volume":"39 6","pages":"612-613"},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139478653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pathological Changes and Cause of Death Associated with the Global Novel Coronavirus Disease (COVID-19). 与全球新型冠状病毒病(COVID-19)相关的病理变化和死因。
Journal of Forensic Medicine Pub Date : 2023-12-25 DOI: 10.12116/j.issn.1004-5619.2023.430703
Bin Yang, Lu-Yao Xu, Ling-Yue Li, Dong-Fang Qiao, Si-Hao DU, Xia Yue, Hui-Jun Wang
{"title":"Pathological Changes and Cause of Death Associated with the Global Novel Coronavirus Disease (COVID-19).","authors":"Bin Yang, Lu-Yao Xu, Ling-Yue Li, Dong-Fang Qiao, Si-Hao DU, Xia Yue, Hui-Jun Wang","doi":"10.12116/j.issn.1004-5619.2023.430703","DOIUrl":"10.12116/j.issn.1004-5619.2023.430703","url":null,"abstract":"<p><p>The coronavirus disease 2019 (COVID-19) has been a global epidemic for more than three years, causing more than 6.9 million deaths. COVID-19 has the clinical characteristics of strong infectivity and long incubation period, and can cause multi-system damage, mainly lung damage, clinical symptoms of acute respiratory distress syndrome (ARDS) and systemic multiple organ damage. The SARS-CoV-2 virus is still constantly mutating. At present, there is no global consensus on the pathological changes of COVID-19 associated deaths and even no consensus on the criteria for determining the cause of death. The investigation of the basic pathological changes and progression of the disease is helpful to guide the clinical treatment and the development of therapeutic drugs. This paper reviews the autopsy reports and related literature published worldwide from February 2020 to June 2023, with a clear number of autopsy cases and corresponding pathological changes of vital organs as the inclusion criteria. A total of 1 111 autopsy cases from 65 papers in 18 countries are included. Pathological manifestations and causes of death are classified and statistically analyzed, common pathological changes of COVID-19 are summarized, and analytical conclusions are drawn, suggesting that COVID-19 infection can cause life-threatening pathological changes in vital organs. On the basis of different health levels of infected groups, the direct cause of death is mainly severe lung damage and secondary systemic multiple organ failure.</p>","PeriodicalId":15899,"journal":{"name":"Journal of Forensic Medicine","volume":"39 6","pages":"586-595"},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139478691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Proteomic Difference Analysis of Whole Blood and Bloodstains. 全血和血迹的蛋白质组差异分析
Journal of Forensic Medicine Pub Date : 2023-12-25 DOI: 10.12116/j.issn.1004-5619.2022.521204
Ao Huang, Shu-Bo Wen, Qian-Qian Kong, Zhen-Min Zhao, Xi-Ling Liu
{"title":"Proteomic Difference Analysis of Whole Blood and Bloodstains.","authors":"Ao Huang, Shu-Bo Wen, Qian-Qian Kong, Zhen-Min Zhao, Xi-Ling Liu","doi":"10.12116/j.issn.1004-5619.2022.521204","DOIUrl":"10.12116/j.issn.1004-5619.2022.521204","url":null,"abstract":"<p><strong>Objectives: </strong>To study the changes of protein levels in peripheral blood after it dried.</p><p><strong>Methods: </strong>The proteins from whole blood and bloodstains were detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and normalized by the label-free quantification (LFQ) method. The differential proteins were analyzed by using R 4.2.1 software, limma and edgeR package. The analysis of biological function, signaling pathway and subcellular localization for the differential proteins was then performed.</p><p><strong>Results: </strong>A total of 623 and 596 proteins were detected in whole blood and bloodstains, respectively, of which 31 were statistically significant in the quantitative results, including 10 up-regulated and 21 down-regulated proteins in bloodstains.</p><p><strong>Conclusions: </strong>The protein abundances in whole blood and bloodstains are highly correlated, and the variation of protein abundances may be related to the changes of endogenous and structural proteins in cells. The application of proteomics technology can assist the screening and identification of protein biomarkers, thereby introducing new biomarkers for forensic research.</p>","PeriodicalId":15899,"journal":{"name":"Journal of Forensic Medicine","volume":"39 6","pages":"549-556"},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139478692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Traffic accident caused by acute attack of Hashimoto encephalopathy: A case report]. [桥本脑病急性发作引发的交通事故:病例报告]。
Journal of Forensic Medicine Pub Date : 2023-12-25 DOI: 10.12116/j.issn.1004-5619.2022.120301
德 凤 胡
{"title":"[Traffic accident caused by acute attack of Hashimoto encephalopathy: A case report].","authors":"德 凤 胡","doi":"10.12116/j.issn.1004-5619.2022.120301","DOIUrl":"10.12116/j.issn.1004-5619.2022.120301","url":null,"abstract":"","PeriodicalId":15899,"journal":{"name":"Journal of Forensic Medicine","volume":"39 6","pages":"635-638"},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139478578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Traceability of Geographic Origin Using Human Skin and Oral Microbiota. 利用人体皮肤和口腔微生物群追溯地理来源。
Journal of Forensic Medicine Pub Date : 2023-12-25 DOI: 10.12116/j.issn.1004-5619.2023.530401
Xin-Yu Dong, Ru-Xin Zhu, Yin-Lei Lei, Rui-Yang Tao, Cheng-Tao Li
{"title":"Traceability of Geographic Origin Using Human Skin and Oral Microbiota.","authors":"Xin-Yu Dong, Ru-Xin Zhu, Yin-Lei Lei, Rui-Yang Tao, Cheng-Tao Li","doi":"10.12116/j.issn.1004-5619.2023.530401","DOIUrl":"10.12116/j.issn.1004-5619.2023.530401","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the possibility of using human skin and oral microorganisms to estimate the geographic origin of an individual through the sequencing analysis of bacterial 16S rRNA gene.</p><p><strong>Methods: </strong>Microbial DNA was extracted from the palm and oral microorganisms of the Han population in Shanghai and Chifeng, Inner Mongolia, and the composition and diversity of the microbiota were analyzed by full-length 16S rRNA gene sequencing. Then, differential species were screened and a geographic location prediction model was constructed.</p><p><strong>Results: </strong>The compositions of palm and oral microorganisms between Shanghai and Chifeng samples were both different. The abundance and uniformity of palm side skin microorganisms were higher in Chifeng samples than in Shanghai samples, while there was no significant difference in oral microorganisms. Permutational multivariate analysis of variance (PERMANOVA) confirmed that th<b>e</b> β<b>-</b>diversity between the samples from the two places were statistically significant, and the coefficients of determination (<i>R</i><sup>2</sup>) for skin and oral samples were 0.129 and 0.102, respectively. Through principal co-ordinates analysis (PCoA), the samples from the two places could be preliminarily distinguished. The predictive model had the accuracies of 0.90 and 0.83 for the geographic origin using the skin and oral samples, respectively.</p><p><strong>Conclusions: </strong>There are differences in the compositions of palm and oral microbiota between Han populations in Shanghai and Chifeng. The prediction model constructed by the random forest algorithm can trace the unknown individuals from the above two places.</p>","PeriodicalId":15899,"journal":{"name":"Journal of Forensic Medicine","volume":"39 6","pages":"557-563"},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139478695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application of Targeted Coronary Angiography in the Diagnosis of Sudden Cardiac Death. 靶向冠状动脉造影在诊断心脏性猝死中的应用。
Journal of Forensic Medicine Pub Date : 2023-12-25 DOI: 10.12116/j.issn.1004-5619.2022.421108
Xin Zhao, Zhi-Ming Chen, Wen-Yun Liu, Bo Wang, Hong-Yang Li, Li-Yao Yang, Yan Teng, Li-Jun Wang, Yan-Bin Gao, Wei-Long Chen, Lei Zhang
{"title":"Application of Targeted Coronary Angiography in the Diagnosis of Sudden Cardiac Death.","authors":"Xin Zhao, Zhi-Ming Chen, Wen-Yun Liu, Bo Wang, Hong-Yang Li, Li-Yao Yang, Yan Teng, Li-Jun Wang, Yan-Bin Gao, Wei-Long Chen, Lei Zhang","doi":"10.12116/j.issn.1004-5619.2022.421108","DOIUrl":"10.12116/j.issn.1004-5619.2022.421108","url":null,"abstract":"<p><strong>Objectives: </strong>To diagnose coronary artery stenosis by using the postmortem computed tomography angiography (PMCTA), and to explore the diagnostic value of PMCTA in sudden cardiac death.</p><p><strong>Methods: </strong>Six death cases were selected, and the contrast medium iohexol was injected under high pressure through femoral artery approach with 5F pigtail catheter to obtain coronary image data and then the data was analyzed. The results of targeted coronary imaging and coronary artery calcium score (CaS) were compared with the results of conventional autopsy and histopathological examination.</p><p><strong>Results: </strong>The autopsy and histopathological examination of cases with coronary artery stenosis obtained similar results in targeted coronary angiography, with a diagnostic concordance rate of 83.3%. Targeted coronary angiography could effectively show coronary artery diseases, and the CaS was consistent with the results of conventional autopsy and histopathological examination.</p><p><strong>Conclusions: </strong>Targeted coronary angiography can be used as an effective auxiliary method for conventional autopsy in cases of sudden cardiac death.</p>","PeriodicalId":15899,"journal":{"name":"Journal of Forensic Medicine","volume":"39 6","pages":"542-548"},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139478686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[eath from rupture of penetrating atherosclerotic ulcer of thoracic aorta:A case report]. [胸主动脉穿透性动脉粥样硬化溃疡破裂致死:病例报告]。
Journal of Forensic Medicine Pub Date : 2023-12-25 DOI: 10.12116/j.issn.1004-5619.2022.420809
玮 玮 朱, 晨 光 杨, 美 辰 潘, 天 迎 孙, 慧 讷 刘, 红 梅 董
{"title":"[eath from rupture of penetrating atherosclerotic ulcer of thoracic aorta:A case report].","authors":"玮 玮 朱, 晨 光 杨, 美 辰 潘, 天 迎 孙, 慧 讷 刘, 红 梅 董","doi":"10.12116/j.issn.1004-5619.2022.420809","DOIUrl":"10.12116/j.issn.1004-5619.2022.420809","url":null,"abstract":"","PeriodicalId":15899,"journal":{"name":"Journal of Forensic Medicine","volume":"39 6","pages":"617-619"},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139478654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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