Journal of fish diseases最新文献

{"title":"The Pathology and Blood Biochemistry of Juvenile Lates calcarifer on Diets Contaminated With Mycotoxins, Histamines and Rancid Fats-A Case Study.","authors":"Aidan Kwok, Jose A Domingos, Joseph Oh, Piero Chan, Joyce J L Ong, Leo Nankervis, Jeffrey Ling, Robert Nichol, Susan Gibson-Kueh","doi":"10.1111/jfd.14034","DOIUrl":"https://doi.org/10.1111/jfd.14034","url":null,"abstract":"<p><p>Mycotoxins, originating from contaminated raw materials or suboptimal feed storage, are a growing concern in tropical aquaculture. Common fungi such as Aspergillus spp. and Fusarium spp. produce mycotoxins including aflatoxin, fumonisin, deoxynivalenol and zearalenone. High doses or prolonged exposure (weeks) to low doses of these mycotoxins (< 20 μg/kg) can depress growth, immunity, and cause mortality. This study investigated poor growth and low survival in juvenile Asian seabass, Lates calcarifer fed two different diets (FM40 and ABS3) for 5 weeks. Analysis of these diets revealed high peroxide values, multiple mycotoxins and high histamine levels. Fish fed the FM40 diet, which was contaminated with aflatoxin B1 (13.2 μg/kg), aflatoxin B2 (1.9 μg/kg), deoxynivalenol (29.5 μg/kg), alternariol (2.2 μg/kg), elevated peroxide value (45.91 mEq/kg), and histamine (129.51 mg/kg) developed mild bile duct hyperplasia, depressed total serum proteins (50.40 ± 10.06 g/L), markedly elevated blood potassium (8.2 ± 0.18 mmol/L), and heavy iron deposits in splenic melanomacrophage centres (Perl's stain) indicative of increased haemolysis. The presence of multiple cytotoxic mycotoxins in FM40 diet could explain the increased haemolysis and elevated blood potassium. In contrast, fish fed the ABS3 diet, which had high histamine levels (210.05 mg/kg), exhibited protein-losing nephropathy with multifocal fibrin plugs (Martius scarlet blue stain) indicating acute renal damage, and elevated blood calcium and phosphorus levels. Histamine is metabolised and excreted through the kidneys and known to induce renal arteriolar constriction, disrupt glomerular filtration barrier and increase permeability resulting in protein loss. This study shows that blood biochemistry and histopathology are useful diagnostic tools for assessing the impact of mycotoxins and histamines on fish health.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14034"},"PeriodicalIF":2.2,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune Response of Silver Pomfret (Pampus argenteus) CC Chemokine Ligand Gene Family to Photobacterium damselae Subsp. Damselae and Nocardia seriolae Infections","authors":"Kaiheng Yan,&nbsp;Youyi Zhang,&nbsp;Yuanbo Li,&nbsp;Mengke Tang,&nbsp;Yifan Xu,&nbsp;Xiaojun Yan,&nbsp;Jiabao Hu,&nbsp;Yajun Wang","doi":"10.1111/jfd.14032","DOIUrl":"10.1111/jfd.14032","url":null,"abstract":"<div>\u0000 \u0000 <p>Chemokines play a crucial role in immune responses by facilitating the migration of cells expressing corresponding chemokine receptors along concentration gradients. <i>Photobacterium damselae</i> subsp. <i>Damselae</i> (PDD) and <i>Nocardia seriolae</i> (NS) are known to induce substantial mortality in silver pomfret populations, yet there exists a dearth of research regarding the immune response of <i>CCL</i>s in PDD- or NS-infected silver pomfret. In our investigation, we identified 10 <i>PaCCL</i>s, which include one fish-specific <i>CCL</i> (<i>PaCCL44</i>). Phylogenetic analysis revealed considerable diversity in <i>CCL</i> types and copy numbers among various teleost fishes. Notably, silver pomfret lacks specific <i>CCL</i> genes, with most <i>PaCCL</i>s exhibiting heightened expression levels in immune-related organs such as the spleen and kidney, and some being expressed in mucosal immune-related organs like the skin and gills. Transcriptome analysis conducted on silver pomfret infected with NS and PDD elucidated that the expression changes of <i>PaCCL</i>s primarily manifested in the spleen during the initial stages of NS infection, shifting to the kidney in later stages. Conversely, the expression changes of <i>PaCCL</i>s following PDD infection predominantly occurred in the kidney. In vitro studies using silver pomfret spleen cell lines demonstrated an early peak in <i>PaCCL</i>s expression during infection, followed by gradual decline with NS treatment and rapid diminishment with PDD treatment. These findings suggest that <i>PaCCL</i>s primarily support the innate immunity of silver pomfret, potentially exhibiting chemotactic effects in the early infection stages, such as the synergistic action of <i>PaCCL4</i> and <i>PaCCL25</i>, and later serving as direct antibacterial agents. NS invasion is characterised by a chronic infection affecting multiple organs, whereas PDD primarily inflicts severe damage to the kidney. <i>PaCCL19a</i> and <i>PaCCL19b</i> are specific to PDD, and their expression levels may decrease in the later stages of infection due to PDD immune escape. These data offer initial insights into understanding the mechanism underlying the innate immune response of the <i>CCL</i> gene family in silver pomfret and provide theoretical underpinnings for fish culture practices.</p>\u0000 </div>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"48 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors Influencing the Prevalence of Hyperpigmented Melanistic Lesions in Smallmouth Bass Micropterus dolomieu in the Susquehanna River Basin, Pennsylvania","authors":"Megan K. Schall,&nbsp;Geoffrey D. Smith,&nbsp;Vicki S. Blazer,&nbsp;Heather L. Walsh,&nbsp;Tyler Wagner","doi":"10.1111/jfd.14033","DOIUrl":"10.1111/jfd.14033","url":null,"abstract":"<p>Hyperpigmented melanistic lesions (HPMLs) are a visual anomaly documented on the skin of smallmouth bass <i>Micropterus dolomieu</i> in the Susquehanna River Basin, Pennsylvania and in numerous other geographical locations. Currently, there is a lack of information on environmental and fish characteristics that may influence the prevalence of HPMLs associated with a recently described <i>Adomavirus</i>. The goal of this study was to understand potential drivers associated with HPMLs in socioeconomically and ecologically important riverine smallmouth bass populations. A total of 16,220 smallmouth bass were collected and examined for HPMLs between 2012 and 2022 in the Susquehanna River Basin. Overall, HPMLs were documented on 2.9% of fish collected. The interaction between temperature and fish size suggested differing relationships between shorter and longer fish with respect to temperature. Predicted probability of HPML prevalence ranged from 1.1% (95% CI = 0.3, 3.2) at 4°C to 0.01% (CI = 0.00, 0.04) at 26°C for an age-0 (125 mm) fish. In contrast, predicted probability of HPML prevalence ranged from 10.5% (95% CI = 5.8, 18.9) at 4°C to 0.8% (CI = 0.4, 1.5) at 26°C for an adult (322 mm) fish. Overall, HPMLs were more common in longer fish during cooler temperature periods which also corresponds to key life history periods for smallmouth bass (e.g., pre-spawn and overwintering) and could represent different exposure histories for juvenile and adult fish.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"48 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jfd.14033","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Constituents From Brazilian Propolis Against Edwardsiella ictaluri and Flavobacterium covae, Two Bacteria Affecting Channel Catfish (Ictalurus punctatus).","authors":"Victor P Ribeiro, Jairo K Bastos, Marcuslene D Harries, Phaedra N Page, Natascha Techen, Kumudini M Meepagala","doi":"10.1111/jfd.14030","DOIUrl":"https://doi.org/10.1111/jfd.14030","url":null,"abstract":"<p><p>Edwardsiella ictaluri and Flavobacterium covae are two bacteria species that cause diseases in farm-raised channel catfish (Ictalurus punctatus) that cause heavy economic damage to the aquaculture industry, particularly to the channel catfish farming. In search for environmentally benign antibacterial compounds active against E. ictaluri and F. covae, we investigated the constituents isolated from Brazilian red, brown and green propolis. We have also synthetically modified active constituents to see if lipophilicity plays a role in enhancing antibacterial activities. Vestitol, neovestitol and methylvestitol were found to be the active constituents with minimum inhibitory concentration (MIC) relative to drug control florfenicol (RDCF) values (MIC^-RDCF) of 7.6, 7.6 and 7.9 mg/L, respectively, against F. covae. The activity against E. ictaluri was not significant.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14030"},"PeriodicalIF":2.2,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Exposure Dosage and Host Genetics on the Shedding Kinetics of Flavobacterium psychrophilum in Rainbow Trout.","authors":"Darbi R Jones, Jeremy Everson, Timothy D Leeds, Gregory D Wiens, Andrew R Wargo","doi":"10.1111/jfd.14026","DOIUrl":"https://doi.org/10.1111/jfd.14026","url":null,"abstract":"<p><p>Flavobacterium psychrophilum, the causative agent of bacterial cold water disease (BCWD), is one of the leading pathogens in rainbow trout (Oncorhynchus mykiss) aquaculture. To date, there is little knowledge of the transmission kinetics of F. psychrophilum over the course of infection. In particular, how transmission is affected by host genotype and pathogen exposure dosage are not well studied. In order to fill in these knowledge gaps, we exposed two divergently selected lines of rainbow trout (ARS-Fp-R and ARS-Fp-S) to a range of dosages of F. psychrophilum (strain CSF117-10). We then measured mortality and bacterial shedding to estimate transmission risk at multiple time points since initial infection. As dosage increased, the number of fish shedding and the amount of bacteria shed increased ranging from 0% to 100% and 10³ to 10⁸ cells fish^-1 h^-1, respectively. In addition, we found that disease resistance (survival) was not correlated with transmission risk blocking, in that 67% of fish which shed bacteria experienced no clinical disease. In general, fish mortality began on Day 3, peaked between Days 5-7 and was higher in the ARS-Fp-R line. Results from this study could be used to develop epidemiological models and improve disease management, particularly in the context of aquaculture and selective breeding.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14026"},"PeriodicalIF":2.2,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Classification of Kudoa thyrsites infected and uninfected fish using a handheld near-infrared spectrophotometer, SIMCA and PLS-DA","authors":"S. Henning","doi":"10.1111/jfd.14025","DOIUrl":"10.1111/jfd.14025","url":null,"abstract":"<p><i>Kudoa thyrsites</i> infection of marine fish typically results in myoliquefaction, which is only apparent 24 to 56 h <i>post-mortem</i>. The traditional methods for the detection of <i>K. thyrsites</i> infected fish are time-consuming and destructive, reducing its marketability. This poses a challenge for the fish industry to remove infected fish before it reaches the market or further processing activities. This study investigated the use of near-infrared (NIR) spectroscopy, in combination with soft independent modelling of class analogy (SIMCA) and partial least square discriminant analysis (PLS-DA), for discriminating <i>K. thyrsites</i> infected fish from uninfected fish. Performance of the classification models was evaluated by calculating the sensitivity, specificity and precision. A total of 334 fish samples (200 sardine, 64 hake and 70 kingklip) were used for this study. Infection of <i>K. thyrsites</i> was determined with the use of qPCR assays. Ninety per cent (90%) of the sardine samples, 78% of the hake samples and 37% of the kingklip samples were infected. Class groups of infected and uninfected fish samples were created for the purpose of generating SIMCA and PLS-DA classification models for each species of fish, as well as for a species independent data set. Principal component analysis (PCA) of NIR spectra did not show any clustering for infected and uninfected samples. Calibration and test sample sets were generated for the purpose of building and testing the SIMCA and PLD-DA classification models. SIMCA and PLS-DA were unable to classify test samples correctly into the two classes. The number of misclassifications (NMC) was higher for the SIMCA models than for the PLS-DA models, with more than 60% incorrectly classified. SIMCA classified most of the test samples into both classes. The precision for PLS-DA were 89% for sardine, 81% for hake, 0% for kingklip and 87% for species independent models, however, most samples were classified at infected. The use of NIR spectroscopy and classification models such as SIMCA and PLS-DA showed limited use as a method to distinguish between <i>K. thyrsites</i> infected and uninfected fish samples. Textural and chemical changes during extended frozen storage of the fish samples may have masked the effects associated with <i>K. thyrsites</i> infection. Further studies are suggested where NIR spectroscopy is used in combination with texture analysis and image spectroscopy.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"48 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jfd.14025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AI-Driven Realtime Monitoring of Early Indicators for Ichthyophthirius multifiliis Infection of Rainbow Trout","authors":"Rikke Bonnichsen,&nbsp;Glenn Gunner Brink Nielsen,&nbsp;Jeppe Seidelin Dam,&nbsp;Dorte Schrøder-Petersen,&nbsp;Kurt Buchmann","doi":"10.1111/jfd.14027","DOIUrl":"10.1111/jfd.14027","url":null,"abstract":"<p>A novel video-based real-time system based on AI (artificial intelligence) was developed to detect clinical signs in fish exposed to pathogens. We selected a White Spot Disease model involving rainbow trout as the experimental animal and the parasitic ciliate <i>Ichthyophthirius multifiliis</i> as a pathogen. We compared two identical fish tank systems: one tank was infected by co-habitation, whereas the other tank was kept non-infected (sham infection). The two fish tanks were separately video monitored (full top and side view) during the course of infection, during which fish were removed whenever they developed clinical signs (direct visual inspection by the observer). Image analysis (object detection, classification and tracking) was used to track behavioural changes in fish (in every recorded video frame), focusing on movement patterns and spatial localisation. Initially, the two fish groups (infected and non-infected) exhibited similar behaviour and non-infected fish did not change behaviour during the 15 d observation period (from 5 d before infection until 10 dpi). At 4, 7, 8, 9 and 10 dpi some infected fish showed clinical signs (equilibrium disturbance, gasping and lethargy) and were removed from the experiment. Anorexia occurred from 5 dpi and a gradual progression of gasping behaviour was noted, whereas the frequency of fish flashing (rubbing/scratching against objects) was low. Equilibrium disturbances and the development of white spots in the skin appeared to be a much later (8–10 dpi at this temperature) indicator of infection. The video analysis showed a general distribution of non-infected fish in all parts of the fish tank during the entire experiment, whereas infected fish already at 4–5 dpi moved towards higher water currents in the top and bottom positions. This change of fish positioning within the tank appeared as a promising early indicator of infection. The study suggests that continuous monitoring of fish behaviour using AI can potentially optimise the timing of humane endpoints, indicate disease signs earlier and thereby improve animal welfare in both animal experimentation and in aquaculture settings.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"48 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jfd.14027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of cardiac cell cultures from salmonids to measure the cardiotoxic effect of environmental pollutants","authors":"Torben Krebs,&nbsp;Julia Bauer,&nbsp;Sarah Graff,&nbsp;Lukas Teich,&nbsp;Markus Sterneberg,&nbsp;Marina Gebert,&nbsp;Henrike Seibel,&nbsp;Bettina Seeger,&nbsp;Dieter Steinhagen,&nbsp;Verena Jung-Schroers,&nbsp;Mikolaj Adamek","doi":"10.1111/jfd.14018","DOIUrl":"10.1111/jfd.14018","url":null,"abstract":"<p>Environmental stressors such as micro- and nanosized plastic particles (MNPs) or crude oil have a detrimental effect on aquatic animals; however, the impact upon the cardiovascular system of fish remains relatively under-researched. This study presents a novel approach for investigating the effect of crude oil and MNPs on the cardiac system of fish. We used salmonid larvae and cardiac cell cultures derived from hearts of salmonid fish and exposed them to environmental stressors. Following exposure to plastic particles or crude oil, the larvae exhibited some variation in contraction rate. In contrast, significant alterations in the contraction rate were observed in all cardiac cell cultures. The greatest differences between the control and treatment groups were observed in cardiac cell cultures derived from older brown trout. Following 7 days of exposure to MNPs or crude oil in Atlantic salmon larval hearts or cardiac cell cultures, there were only minor responses noted in mRNA expression of the selected marker genes. These findings show the use of a novel in vitro technique contributing to the existing body of knowledge on the impact of MNPs and crude oil on the cardiovascular system of salmonids and the associated risk.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"48 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jfd.14018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mucosal epithelial homeostasis: Reference intervals for skin, gill lamellae and filament for Atlantic salmon and other fish species","authors":"Grigory V. Merkin,&nbsp;Albert Girons,&nbsp;Mearge A. Okubamichael,&nbsp;Karin Pittman","doi":"10.1111/jfd.14023","DOIUrl":"10.1111/jfd.14023","url":null,"abstract":"<p>Mucosal barriers are gatekeepers of health and exhibit homeostatic variation in relation to habitat and disease. Mucosal Mapping technology provides an in-depth examination of the dynamic mucous cells (MCs) in fish mucosal barriers on tangential sections, about 90° from the view of traditional histology. The method was originally developed and standardized in academia prior to the establishment of QuantiDoc AS to apply mucosal mapping, now trademarked as Veribarr™ for the analysis of skin, gills and gastrointestinal tracts. Veribarr™ uses design-based stereology for the selection and measurement of cell area (size) (μm²), the volumetric density of MCs in the epithelium (MCD, amount of the epithelia occupied by MCs, in %) and the calculated abundance of the MCs (barrier status or defence activity). MC production was mapped across the skin and gill epithelia in 12 species, discovering that gills consistently have two distinct groups of MCs, one on the lamellae where MCs are few and small and one on the filament where MCs are larger and more abundant. MCs were usually much larger in the skin than in the gills, with the latter requiring fewer and smaller cells for adequate respiration. The difference observed between MCs in gill lamella and gill filament is likely a result of functional demands. In addition, our findings also highlight a variation in the mucosal parameters between the species skin, which cannot be explained by the weight differences, and a potential link between MC distribution and species-specific lifestyles in the gill lamella. This diversity necessitates the development of species and tissue site-specific reference intervals for mucosal health evaluation. Mucosal bivariate reference intervals were developed for MC production, including size (trophy) and calculated defence activity (plasia) in the skin and gills of Atlantic salmon, to contrast new measurements against historical data patterns. The application of mucosal reference intervals demonstrates that stress from parasites and treatments can manifest as changes in mucosal architecture, as evidenced by MC hypertrophy and hyperplasia within the gill lamellae. These reference intervals also facilitate comparisons with wild Atlantic salmon, revealing a somewhat higher MC level in farmed salmon gill lamellae. These findings suggest that MC hyperplasia and hypertrophy in the gills are stress/environmental responses in aquaculture. They also advocate for developing specific mucosal bivariate homeostatic reference intervals in aquaculture to improve fish health and welfare across all farmed species.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"48 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature-dependent alterations in the proteome of the emergent fish pathogen Edwardsiella piscicida.","authors":"Kim L Jacobsen, Matt Griffin, Brett S Phinney, Michelle Salemi, Zeinab Yazdi, Sujita Balami, Caitlin E Older, Esteban Soto","doi":"10.1111/jfd.14017","DOIUrl":"10.1111/jfd.14017","url":null,"abstract":"<p><p>Edwardsiella piscicida is an emerging bacterial pathogen and the aetiological agent of edwardsiellosis among cultured and wild fish species globally. The increased frequency of outbreaks of this Gram-negative, facultative intracellular pathogen pose not only a threat to the aquaculture industry but also a possible foodborne/waterborne public health risk due to the ill-defined zoonotic potential. Thus, understanding the role of temperature on the virulence of this emerging pathogen is essential for comprehending the pathogenesis of piscine edwardsiellosis in the context of current warming trends associated with climate change, as well as providing insight into its zoonotic potential. In this study, significant temperature-dependent alterations in bacterial growth patterns were observed, with bacterial isolates grown at 17°C displaying higher peak growth sizes, extended lag times, and slower maximal growth rates than isolates grown at 27or 37°C. When E. piscicida isolates were grown at 37°C compared to 27 and 17°C, mass spectrometry analysis of the E. piscicida proteome revealed significant downregulation of crucial virulence proteins, such as Type VI secretion system proteins and flagellar proteins. Although in vivo models of infection are warranted, this in vitro data suggests possible temperature-associated alterations in the virulence and pathogenic potential of E. piscicida in poikilotherms and homeotherms.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14017"},"PeriodicalIF":2.2,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}