Journal of General and Applied Microbiology最新文献_第5页

Artificial activation of both σ^H and Spo0A in Bacillus subtilis enforced initiation of spore development at the vegetatively growing phase. 人工激活枯草芽孢杆菌中的σH和Spo0A，可在无性生殖阶段启动孢子发育。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2024-02-02 Epub Date: 2023-06-29 DOI: 10.2323/jgam.2023.06.004

Tomomitsu Karaki, Ai Sunaga, Yasuhiro Takahashi, Kei Asai

{"title":"Artificial activation of both σH and Spo0A in Bacillus subtilis enforced initiation of spore development at the vegetatively growing phase.","authors":"Tomomitsu Karaki, Ai Sunaga, Yasuhiro Takahashi, Kei Asai","doi":"10.2323/jgam.2023.06.004","DOIUrl":"10.2323/jgam.2023.06.004","url":null,"abstract":"When Bacillus subtilis cells face environmental deterioration, such as exhaustion of nutrients and an increase in cell density, they form spores. It is known that phosphorylation of Spo0A and activation of σH are key events at the initiation of sporulation. However, the initiation of sporulation is an extremely complicated process, and the relationship between these two events remains to be elucidated. To determine the minimum requirements for triggering sporulation initiation, we attempted to induce cell sporulation at the log phase, regardless of nutrients and cell density. In rich media such as Luria-Bertani (LB) medium, the cells of B. subtilis do not sporulate efficiently, possibly because of excess nutrition. When the amount of xylose in the LB medium was limited, σH -dependent transcription of the strain, in which sigA was under the control of the xylose-inducible promoter, was induced, and the frequency of sporulation was elevated according to the decreased level of σA. We also employed a fusion of sad67, which codes for an active form of Spo0A, and the IPTG-inducible promoter. The combination of lowered σA expression and activated Spo0A allowed the cells in the log phase to stop growing and rush into spore development. This observation of enforced initiation of sporulation in the mutant strain was detected even in the presence of the wild-type strain, suggesting that only intracellular events initiate and fulfill spore development regardless of extracellular conditions. Under natural sporulation conditions, the amount of σA did not change drastically throughout growth. Mechanisms that sequester σA from the core RNA polymerase and help σH to become active exist, but this has not yet been elucidated.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":" ","pages":"215-228"},"PeriodicalIF":1.2,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9748688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of n-butanol production on metabolism and the photosystem in Synecococcus elongatus PCC 7942 based on metabolic flux and target proteome analyses. 基于代谢通量和目标蛋白质组分析的正丁醇生产对 Synecococcus elongatus PCC 7942 的新陈代谢和光合系统的影响。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2024-02-02 Epub Date: 2023-03-20 DOI: 10.2323/jgam.2023.03.002

Keisuke Wada, Kiyoka Uebayashi, Yoshihiro Toya, Sastia Prama Putri, Fumio Matsuda, Eiichiro Fukusaki, James C Liao, Hiroshi Shimizu

{"title":"Effects of n-butanol production on metabolism and the photosystem in Synecococcus elongatus PCC 7942 based on metabolic flux and target proteome analyses.","authors":"Keisuke Wada, Kiyoka Uebayashi, Yoshihiro Toya, Sastia Prama Putri, Fumio Matsuda, Eiichiro Fukusaki, James C Liao, Hiroshi Shimizu","doi":"10.2323/jgam.2023.03.002","DOIUrl":"10.2323/jgam.2023.03.002","url":null,"abstract":"Although n-butanol (BuOH) is an ideal fuel because of its superior physical properties, it has toxicity to microbes. Previously, a Synechococcus elongatus PCC 7942 derivative strain that produces BuOH from CO2 was developed by introducing six heterologous genes (BUOH-SE strain). To identify the bottleneck in BuOH production, the effects of BuOH production and its toxicity on central metabolism and the photosystem were investigated. Parental (WT) and BUOH-SE strains were cultured under autotrophic conditions. Consistent with the results of a previous study, BuOH production was observed only in the BUOH-SE strain. Isotopically non-stationary 13C-metabolic flux analysis revealed that the CO2 fixation rate was much larger than the BuOH production rate in the BUOH-SE strain (1.70 vs 0.03 mmol gDCW-1 h-1), implying that the carbon flow for BuOH biosynthesis was less affected by the entire flux distribution. No large difference was observed in the flux of metabolism between the WT and BUOH-SE strains. Contrastingly, in the photosystem, the chlorophyll content and maximum O2 evolution rate per dry cell weight of the BUOH-SE strain were decreased to 81% and 43% of the WT strain, respectively. Target proteome analysis revealed that the amounts of some proteins related to antennae (ApcA, ApcD, ApcE, and CpcC), photosystem II (PsbB, PsbU, and Psb28-2), and cytochrome b6f complex (PetB and PetC) in photosystems decreased in the BUOH-SE strain. The activation of photosynthesis would be a novel approach for further enhancing BuOH production in S. elongatus PCC 7942.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":" ","pages":"185-195"},"PeriodicalIF":1.2,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9133419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzyme immobilization on α-1,3-glucan: development of flow reactor with fusion protein of α-1,3-glucan binding domains and histamine dehydrogenase. 在α-1,3-葡聚糖上固定酶：利用α-1,3-葡聚糖结合域和组胺脱氢酶的融合蛋白开发流动反应器。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2024-02-02 Epub Date: 2023-05-16 DOI: 10.2323/jgam.2023.04.002

Yuta Nagahashi, Kazuki Hasegawa, Kazuyoshi Takagi, Shigekazu Yano

{"title":"Enzyme immobilization on α-1,3-glucan: development of flow reactor with fusion protein of α-1,3-glucan binding domains and histamine dehydrogenase.","authors":"Yuta Nagahashi, Kazuki Hasegawa, Kazuyoshi Takagi, Shigekazu Yano","doi":"10.2323/jgam.2023.04.002","DOIUrl":"10.2323/jgam.2023.04.002","url":null,"abstract":"α-1,3-Glucanase Agl-KA from Bacillus circulans KA-304 consists of a discoidin domain (DS1), a carbohydrate binding module family 6 (CBM6), a threonine-proline-rich-linker (TP linker), a discoidin domain (DS2), an uncharacterized domain, and a catalytic domain. The binding of DS1, CBM6, and DS2 to α-1,3-glucan can be improved in the presence of two of these three domains. In this study, DS1, CBM6, and TP linker were genetically fused to histamine dehydrogenase (HmDH) from Nocardioides simplex NBRC 12069. The fusion enzyme, AGBDs-HmDH, was expressed in Escherichia coli Rosetta 2 (DE3) and purified from the cell-free extract. AGBDs-HmDH bound to 1% micro-particle of α-1,3-glucan (diameter: less than 1 μm) and 7.5% coarse-particle of α-1,3-glucan (less than 200 μm) at about 97 % and 70% of the initial amounts of the enzyme, respectively. A reactor for flow injection analysis filled with AGBDs-HmDH immobilized on the coarse-particle of α-1,3-glucan was successfully applied to determine histamine. A linear calibration curve was observed in the range for about 0.1 to 3.0 mM histamine. These findings suggest that the combination of α-1,3-glucan and α-1,3-glucan binding domains is a candidate for novel enzyme immobilization.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":" ","pages":"206-214"},"PeriodicalIF":1.2,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9833400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effect of secondary metabolites isolated from Pestalotiopsis sp. FKR-0115 in overcoming β-lactam resistance in MRSA. 从Pestalotiopsis sp.FKR-0115中分离出的次生代谢物在克服MRSA对β-内酰胺耐药性方面的协同作用。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2024-02-02 Epub Date: 2023-06-10 DOI: 10.2323/jgam.2023.06.001

Kanako Taba, Masako Honsho, Yukihiro Asami, Hiromu Iwasaki, Kenichi Nonaka, Yoshihiro Watanabe, Masato Iwatsuki, Hidehito Matsui, Hideaki Hanaki, Toshiaki Teruya, Takahiro Ishii

引用次数: 0

TrLys9 participates in fungal development and lysine biosynthesis in Trichoderma reesei. TrLys9参与里氏木霉的真菌发育和赖氨酸的生物合成。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2023-12-05 Epub Date: 2023-02-17 DOI: 10.2323/jgam.2023.01.002

Jinling Lan, Lin Zhang, Jie Gao, Ronglin He

{"title":"TrLys9 participates in fungal development and lysine biosynthesis in Trichoderma reesei.","authors":"Jinling Lan, Lin Zhang, Jie Gao, Ronglin He","doi":"10.2323/jgam.2023.01.002","DOIUrl":"10.2323/jgam.2023.01.002","url":null,"abstract":"Fungi uniquely synthesize lysine through the α-aminoadipate pathway. The saccharopine reductase ScLys9 catalyzes the formation of saccharopine from ɑ-aminoadipate 6-semialdehyde, the seventh step in the lysine biosynthesis pathway in Saccharomyces cerevisiae. Here, we characterized the functions of TrLys9, an ortholog of S. cerevisiae ScLys9 in the industrial filamentous fungus Trichoderma reesei. Transcriptional level analysis indicated that TrLYS9 expression was higher in the conidial stage than in other stages. Disruption of TrLYS9 led to lysine auxotrophy. Phenotype analysis of the ΔTrlys9 mutant showed that TrLYS9 was involved in fungal development including vegetative growth, conidiation, and conidial germination and lysine biosynthesis. Cellulase production was also impaired in the ΔTrlys9 mutant due to the failure of conidial germination in liquid cellulase-inducing medium. Defects in radial growth and asexual development of the ΔTrlys9 mutant were fully recovered when exogenous lysine was added to the medium. These results imply that TrLys9 is involved in fungal development and lysine biosynthesis in T. reesei.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":" ","pages":"159-166"},"PeriodicalIF":1.2,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10757885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of glyceroglycolipids from Lactiplantibacillus pentosus and other Lactiplantibacillus species. 戊糖乳杆菌与其他乳杆菌甘油三酯的比较分析。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2023-12-05 Epub Date: 2022-12-08 DOI: 10.2323/jgam.2022.12.001

Ayako Kuri, Hirokazu Iida, Kazuyoshi Kawahara

{"title":"Comparative analysis of glyceroglycolipids from Lactiplantibacillus pentosus and other Lactiplantibacillus species.","authors":"Ayako Kuri, Hirokazu Iida, Kazuyoshi Kawahara","doi":"10.2323/jgam.2022.12.001","DOIUrl":"10.2323/jgam.2022.12.001","url":null,"abstract":"Cellular lipids of Lactiplantibacillus species were extracted and neutral glyceroglycolipids (GGLs) were purified, and analyzed by thin-layer chromatography (TLC). Four GGLs with known structures were detected in GGL preparation of L. plantarum, and the same GGL profiles of TLC were observed for all other strains of Lactiplantibacillus species examined, suggesting that the GGL profile could be one of the chemotaxonomic characters of the genus Lactiplantibacillus. On the other hand, the quantity of each GGL showed some variation among species, and L. pentosus was found to have higher proportion of disaccharide-type GGL, designated GGL-3 in this study, compared with other species including L. plantarum. The quantitative difference of GGL-3 found in this study could be regarded as the characteristics of L. pentosus. The carbohydrate structure of L. pentosus GGL-3 was precisely analyzed by 1H NMR and methylation analysis, and the structure was confirmed to be αGal-(1→2)-αGlc-diacylglycerol, with the carbohydrate structure identical to that of L. plantarum, although fatty acid composition of the two GGL-3 showed some difference.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":" ","pages":"135-141"},"PeriodicalIF":1.2,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10370805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of three γ-glutamyltranspeptidases from Pseudomonas aeruginosa PAO1. 铜绿假单胞菌PAO1中三个γ-谷氨酰转肽酶的鉴定

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2023-12-05 Epub Date: 2023-01-18 DOI: 10.2323/jgam.2023.01.001

Yuuki Nonomura, Xinjia Wang, Takeshi Kikuchi, Daisuke Matsui, Yosuke Toyotake, Kazuyoshi Takagi, Mamoru Wakayama

{"title":"Characterization of three γ-glutamyltranspeptidases from Pseudomonas aeruginosa PAO1.","authors":"Yuuki Nonomura, Xinjia Wang, Takeshi Kikuchi, Daisuke Matsui, Yosuke Toyotake, Kazuyoshi Takagi, Mamoru Wakayama","doi":"10.2323/jgam.2023.01.001","DOIUrl":"10.2323/jgam.2023.01.001","url":null,"abstract":"The Pseudomonas aeruginosa strain, PAO1, has three putative γ-glutamyltranspeptidase (GGT) genes: ggtI, ggtII, and ggtIII. In this study, the expression of each of these genes in P. aeruginosa PAO1 was analyzed, and the properties of the corresponding GGT proteins were investigated. This is the first report on biochemical characterization of GGT paralogs from Pseudomonas species. The crude extracts prepared from P. aeruginosa PAO1 exhibited hydrolysis and transpeptidation activities of 17.3 and 65.0 mU/mg, respectively, and the transcription of each gene to mRNA was confirmed by RT-PCR. All genes were cloned, and the expression plasmids constructed were introduced into an Escherichia coli expression system. Enzyme activity of the expressed protein of ggtI (PaGGTI) was not detected in the system, while the enzyme activities of the expressed proteins derived from ggtII and ggtIII (PaGGTII and PaGGTIII, respectively) were detected. However, the enzyme activity of PaGGTII was very low and easily decreased. PaGGTII with C-terminal his-tag (PaGGTII25aa) showed increased activity and stability, and the purified enzyme consisted of a large subunit of 40 kDa and a small subunit of 28 kDa. PaGGTIII consisted of a large subunit of 37 kDa and a small subunit of 24 kDa. The maximum hydrolysis and transpeptidation activities of PaGGTII25aa were obtained at 40ºC-50ºC, and the maximum hydrolysis and transpeptidation activities of PaGGTIII were obtained at 50ºC-60ºC. These enzymes retained approximately 80% of their hydrolysis and transpeptidation activities after incubation at 50ºC for 10 min, reflecting good stability. Both PaGGTII25aa and PaGGTIII showed higher activities of hydrolysis and transpeptidation in the alkali range than in the acidic range. However, they were highly stable at a wide pH range (5-10.5).","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":" ","pages":"150-158"},"PeriodicalIF":1.2,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10599088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Bacillus velezensis S141, a soybean growth-promoting bacterium, hydrolyzes isoflavone glycosides into aglycones. velezensis S141是一种促进大豆生长的细菌，它能将异黄酮苷水解成苷元。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2023-12-05 Epub Date: 2023-02-28 DOI: 10.2323/jgam.2023.02.002

Takahiko Kondo, Surachat Sibponkrung, Ken-Yu Hironao, Panlada Tittabutr, Nantakorn Boonkerd, Shu Ishikawa, Hitoshi Ashida, Neung Teaumroong, Ken-Ichi Yoshida

{"title":"Bacillus velezensis S141, a soybean growth-promoting bacterium, hydrolyzes isoflavone glycosides into aglycones.","authors":"Takahiko Kondo, Surachat Sibponkrung, Ken-Yu Hironao, Panlada Tittabutr, Nantakorn Boonkerd, Shu Ishikawa, Hitoshi Ashida, Neung Teaumroong, Ken-Ichi Yoshida","doi":"10.2323/jgam.2023.02.002","DOIUrl":"10.2323/jgam.2023.02.002","url":null,"abstract":"Bacillus velezensis S141, a plant growth-promoting rhizobacteria (PGPR), was isolated from a soybean field in Thailand. Previous studies demonstrated that S141 enhanced soybean growth, stimulating nodulation for symbiotic nitrogen fixation with soybean root nodule bacteria, including Bradyrhizobium diazoefficience USDA110. Isoflavone glycosides are produced in soybean roots and hydrolyzed into their aglycones, triggering nodulation. This study revealed that S141 efficiently hydrolyzed two isoflavone glycosides in soybean roots (daidzin and genistin) to their aglycones (daidzein and genistein, respectively). However, S141, Bacillus subtilis 168, NCIB3610, and B. velezensis FZB42 hydrolyzed isoflavone glucosides into aglycones. A BLASTp search suggested that S141 and the other three strains shared four genes encoding β-glucosidases corresponding to bglA, bglC, bglH, and gmuD in B. subtilis 168. The gene inactivation analysis of B. subtilis 168 revealed that bglC encoded the major β-glucosidase, contributing about half of the total activity to hydrolyze isoflavone glycosides and that bglA, bglH, and gmuD, all barely committed to the hydrolysis of isoflavone glycosides. Thus, an unknown β-glucosidase exists, and our genetic knowledge of β-glucosidases was insufficient to evaluate the ability to hydrolyze isoflavone glycosides. Nevertheless, S141 could predominate in the soybean rhizosphere, releasing isoflavone aglycones to enhance soybean nodulation.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":" ","pages":"175-183"},"PeriodicalIF":1.2,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9379653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A high-temperature sensitivity of Synechococcus elongatus PCC 7942 due to a tRNA-Leu mutation. 长聚球菌PCC 7942因tRNA-Leu突变而对高温敏感。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2023-12-05 Epub Date: 2023-02-17 DOI: 10.2323/jgam.2023.02.001

Hazuki Hasegawa, Yu Kanesaki, Satoru Watanabe, Kan Tanaka

引用次数: 0

Novel transporter screening technology for chemical production by microbial fermentation. 微生物发酵化工生产转运体筛选新技术。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2023-12-05 Epub Date: 2022-12-25 DOI: 10.2323/jgam.2022.12.002

Kei Nanatani, Tomoko Ishii, Ayumu Masuda, Satoshi Katsube, Tasuke Ando, Hiroshi Yoneyama, Keietsu Abe

引用次数: 0