Inhibition and biocontrol potential of Ochrobactrum pseudogrignonense NC1 against four Phytophthora species.

IF 0.8 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of General and Applied Microbiology Pub Date : 2024-05-02 Epub Date: 2023-11-21 DOI:10.2323/jgam.2023.11.001

Jinming Liu, Shiyu Zhang, Haikun Ma, Jun Huang, Meichun Xiang, Xingzhong Liu

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引用次数: 0

Abstract

Phytophthora species are highly destructive soilborne oomycetes pathogens that spread through infested soil and water. Ochrobactrum pseudogrignonense NC1 has been shown to inhibit plant parasitic nematodes via volatile organic compounds (VOCs). In this study, we investigated the inhibitory effect of O. pseudogrignonense NC1 against four Phytophthora species on agar plates and in vivo bioassay. We found that NC1 significantly inhibited the mycelial growth and zoospore production of all four species of Phytophthora in a dose-dependent manner. The half maximal inhibitory concentration (IC₅₀) values for inhibition of mycelial growth (or zoospore production) were 26% (14.8%), 18.9% (14.2%), 20.3% (8.3%) and 46.9% (4%) for Phytophthora capsici Leonian, Phytophthora infestans, Phytophthora parasitica var. nicotiana and Phytophthora sojae, respectively. The biocontrol efficiency of NC1 was 46.3% in pepper seedlings against P. capsici, almost 100% in potato tubers against P. infestans, 60% in tomato leave against P. parasitica and 100% in soybean leave against P. sojae, respectively. Our findings suggest that O. pseudogrignonense NC1 has great potential as a biocontrol agent for managing Phytophthora diseases.

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伪绿僵杆菌NC1对4种疫霉的抑制作用及其生物防治潜力。

疫霉是一种极具破坏性的土传卵菌病原体，通过受感染的土壤和水传播。Ochrobactrum pseudogrignonense NC1通过挥发性有机化合物(VOCs)抑制植物寄生线虫。在本研究中，我们研究了O. pseudogrignonense NC1对4种疫霉菌的抑菌作用，并进行了体内生物测定。我们发现，NC1显著抑制所有四种疫霉菌丝生长和游动孢子产生，并呈剂量依赖性。对辣椒疫霉、鼠疫疫霉、烟草疫霉和大豆疫霉菌丝生长(或虫孢子产生)的半数最大抑制浓度(IC50)值分别为26%(14.8%)、18.9%(14.2%)、20.3%(8.3%)和46.9%(4%)。NC1对辣椒幼苗、马铃薯块茎、番茄叶片和大豆叶片的防虫效果分别为46.3%、100%、60%和100%。我们的研究结果表明，伪grignonense NC1作为防治疫霉病的生物防治剂具有很大的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of General and Applied Microbiology 生物-生物工程与应用微生物

CiteScore

2.40

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.