Journal of Food Safety最新文献

{"title":"Traceable Organization of Food Quality- and Safety-Related Postharvest Evidence in Fresh Tomato Literature","authors":"Xinxing Li,&nbsp;Shuai Yuan,&nbsp;Ruihua Yu,&nbsp;Jianwei Li,&nbsp;Lin Xue,&nbsp;Yunfei Ma","doi":"10.1111/jfs.70089","DOIUrl":"https://doi.org/10.1111/jfs.70089","url":null,"abstract":"<div>\u0000 \u0000 <p>Postharvest evidence relevant to food quality and safety in fresh tomato literature is often scattered across result sections, tables, and figure captions, making condition-specific comparison and evidence trace-back difficult. This study developed and evaluated a traceable approach for organizing such evidence from the literature. The approach combined evidence segmentation, LLM-assisted observation recovery, rule-guided normalization, human review, and structured organization through a Source Article–Evidence Unit–Observation framework. Evaluation was conducted on a small controlled corpus of five English full-text articles, which yielded 17 evidence units and 24 reference observations. The results showed stable performance in identifying evidence units that contained valid observations, whereas field extraction was less consistent and normalization was comparatively robust. The organized observation records supported evidence trace-back and condition-specific comparison across storage-, transport-, packaging-, maturity-, and treatment-related contexts, including observations on firmness, color, weight loss, lycopene, sugar content, microbial count, and pH. Within this controlled setting, the findings support the feasibility of organizing food quality- and safety-related postharvest evidence in fresh tomato literature into traceable and reviewable observation records for literature-based comparison and interpretation. These findings should be understood within the limits of the present small-scale validation and should not yet be generalized to a broader literature domain.</p>\u0000 </div>","PeriodicalId":15814,"journal":{"name":"Journal of Food Safety","volume":"46 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Antimicrobial Resistance of Campylobacter jejuni in Raw Fish Meat and Retail Fish Market Environments in Bareilly, India: Implications for Food Safety From a One Health Surveillance Perspective","authors":"Javed Ahamad Khan,&nbsp;Iqbal Ahmad,&nbsp;Fohad Mabood Husain,&nbsp;Gaurav Kumar,&nbsp;Fahad S. Bazaid,&nbsp;Leena A. Neyaz,&nbsp;Hussein H. Abulreesh","doi":"10.1111/jfs.70090","DOIUrl":"https://doi.org/10.1111/jfs.70090","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Campylobacter jejuni</i> is the leading cause of global foodborne gastroenteritis. The prevalence of this pathogen in nontraditional foods like fish is particularly worrisome. This study investigated the prevalence, antimicrobial resistance, and contamination-associated factors of <i>C. jejuni</i> in raw fish meat and associated retail market environmental samples from a One Health surveillance perspective. A total of 260 samples of raw fish meat (<i>n</i> = 200) and environmental swabs (<i>n</i> = 60) were collected from three major fish markets. Selective culturing, biochemical characterization, and PCR-based <i>hipO</i> gene detection were followed for bacterial isolation and identification. Of the 260 samples examined, 21 (8.08%) samples were positive for <i>C. jejuni</i>, although prevalence varied substantially among sample types, with highest rates observed on chopping boards and floor swabs, and the lowest in raw fish meat. The chopping boards and floor swabs had the highest bacterial prevalence (33.3%), followed by the water buckets (20%). In contrast, a lower <i>C. jejuni</i> prevalence (4.0%) was noted in fish meat. Statistical analysis established a highly significant association between sample type and <i>C. jejuni</i> prevalence (<i>p</i> &lt; 0.001). Antimicrobial susceptibility testing revealed high <i>C. jejuni</i> resistance to cephalothin (100%) and chloramphenicol (71.4%), whereas it was moderately resistant to cotrimoxazole (52.4%) and tetracycline (47.6%). A considerable number of isolates (47.6%, <i>n</i> = 10/21) exhibited multidrug resistance patterns. The findings suggest that contaminated processing environments may contribute to cross-contamination of raw fish during retail handling. The study further highlights the needs for improved sanitation, regular monitoring, and antimicrobial stewardship to attenuate <i>Campylobacter</i> spp.-associated fish-borne infections.</p>\u0000 </div>","PeriodicalId":15814,"journal":{"name":"Journal of Food Safety","volume":"46 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Consumer Use of Package-Level Food Safety Information in Imported Packaged Foods: Perceived Hazards, Label Trust, and Market Entry in Taiwan","authors":"Yan-An Chen,&nbsp;Ming-Chih Fang,&nbsp;Yen-Hua Chen,&nbsp;Pei-Ting Chuang","doi":"10.1111/jfs.70088","DOIUrl":"https://doi.org/10.1111/jfs.70088","url":null,"abstract":"<div>\u0000 \u0000 <p>Imported packaged foods are often purchased under conditions of limited consumer verification, where consumers cannot directly confirm safety-related attributes at the point of purchase and must rely on package-level label information as a visible cue of product identity, disclosure, and apparent safety. This study examined how food safety risk perception, trust in packaging-label information, and food neophilia were associated with consumer engagement with imported packaged foods in Taiwan. A self-administered online survey was completed by 454 adults in Taiwan. The questionnaire assessed perceived food safety hazards, trust in packaging-label information, food neophilia, and imported packaged food purchasing. Descriptive statistics, reliability analysis, bivariate correlations, Friedman tests, a market-entry logistic model, a purchaser-only ordered logistic model, and item-level Mann–Whitney <i>U</i> tests were applied. The highest-rated perceived hazards were unknown ingredients whose safety could not be confirmed, illegal food additives, pesticide residues, natural food toxins, and microbiological contamination. The most trusted package-level label information items were expiry date, ingredient declaration, and disclosure of food additive names. Food safety risk perception was associated with lower odds of market entry, whereas trust in packaging-label information was associated with higher odds of ever having purchased imported packaged foods. Among prior purchasers, food neophilia was associated with higher odds of being in a more frequent purchase category. Item-level comparisons further showed that never-purchasers reported greater concern about all retained food safety risk items, with the largest difference observed for microbiological contamination. These findings suggest that package-level label information functions as a consumer-facing food safety cue, but it should be interpreted as a partial cue rather than as direct evidence of product safety.</p>\u0000 </div>","PeriodicalId":15814,"journal":{"name":"Journal of Food Safety","volume":"46 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148467522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extending the Shelf-Life of Wet Sweet Potato Starch Noodles Through Potassium Cinnamate: Antibacterial Mechanisms and Preservation Efficacy","authors":"You Wu,&nbsp;Sha Yang,&nbsp;Zhigang Chen","doi":"10.1111/jfs.70085","DOIUrl":"https://doi.org/10.1111/jfs.70085","url":null,"abstract":"<div>\u0000 \u0000 <p>Wet sweet potato starch noodles are highly susceptible to microbial spoilage due to their high moisture content, leading to significant economic losses and food safety concerns. This study systematically investigated the bacterial inhibition mechanism of potassium cinnamate, a natural and eco-friendly preservative, against dominant spoilage bacteria in wet sweet potato starch noodles. <i>Pseudomonas aeruginosa</i> and <i>Trichosporon asahii</i> were identified as the predominant spoilage species. Potassium cinnamate exhibited potent in vitro antimicrobial activity with minimum inhibitory concentrations (MICs) of 40 and 20 mg/mL, respectively. The antibacterial effect of potassium cinnamate was concentration-dependent, and when combined with 0.2% citric acid, the shelf life of wet sweet potato starch noodles could extend to 90 days. Mechanistic analyses revealed that potassium cinnamate effectively inhibited microbial growth and biofilm formation by increasing cell membrane permeability, inducing protein leakage, and reducing cell surface hydrophobicity. Morphological alterations, including cell rupture and distortion, were directly visualized via scanning and transmission electron microscopy. These findings demonstrate that potassium cinnamate effectively disrupts membrane integrity and biofilm stability in spoilage microorganisms, providing a scientific basis for its potential application as a green preservative in the food industry.</p>\u0000 </div>","PeriodicalId":15814,"journal":{"name":"Journal of Food Safety","volume":"46 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148324841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and Removal of Multispecies Biofilms Formed by L. monocytogenes, S. aureus, and E. coli","authors":"Viviana Švarcová,&nbsp;Kamila Zdeňková,&nbsp;Simona Peroutková,&nbsp;Marta Štindlová,&nbsp;Ondřej Chlumský,&nbsp;Kateřina Demnerová,&nbsp;Marco A. Lopez Marin","doi":"10.1111/jfs.70087","DOIUrl":"https://doi.org/10.1111/jfs.70087","url":null,"abstract":"<p>This study aimed to characterize mono-, dual-, and triple-species biofilms formed by the bacteria <i>Listeria monocytogenes, Staphylococcus aureus</i>, and <i>Escherichia coli</i>. This was achieved by assessing biofilm formation, planktonic cell growth, biomass, and metabolic activity. In total, 111 biofilm combinations were analyzed. Significant synergistic interactions were observed between <i>S. aureus</i> and <i>L. monocytogenes</i> in dual biofilms. In contrast, <i>E. coli</i> showed no synergy and frequently inhibited biofilm formation when co-cultured with the other species. In triple-species biofilms containing <i>E. coli</i>, biofilm formation decreased by up to 47%, and metabolic activity decreased by over 75%. Furthermore, the effectiveness of selected common disinfectants was evaluated. The effectiveness of solutions containing 0.47% NaClO (chlorine bleach), 8% acetic acid, and 0.5% lactic acid against 24-h biofilms was tested. All disinfectants were effective, with bleach demonstrating the greatest reduction in biomass and metabolic activity. Notably, higher resistance to disinfectants was exhibited by mono-species biofilms than multi-species biofilms in this study. Inter-species interactions strongly modulate biofilm behavior. <i>E. coli</i> tends to suppress biofilm formation, whereas <i>S. aureus</i> and <i>L. monocytogenes</i> consortia promote it. While mono-species biofilms exhibit greater resistance in this study, bleach remains the most effective disinfectant. These results improve our understanding of the dynamics of mixed biofilms and emphasize the importance of selecting appropriate disinfectants for controlling biofilms in the food and healthcare industries.</p>","PeriodicalId":15814,"journal":{"name":"Journal of Food Safety","volume":"46 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jfs.70087","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148324499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nondestructive Detection of Glucocorticoid Residues in Chicken Meat Using Hyperspectral Imaging","authors":"Shijie Xie,&nbsp;Rongchang Jiang,&nbsp;Ronghui Lu,&nbsp;Ke Wang,&nbsp;Chunkui Jin","doi":"10.1111/jfs.70086","DOIUrl":"https://doi.org/10.1111/jfs.70086","url":null,"abstract":"<div>\u0000 \u0000 <p>Residual glucocorticoids in chickens can harm human health, and traditional detection methods can cause environmental pollution. In this study, hyperspectral imaging (HSI) technology was used to investigate the feasibility of nondestructively identifying chicken samples spiked with five different glucocorticoids. First, regions of interest (ROIs) were used to extract hyperspectral data. After preprocessing, the obtained average hyperspectral data were transformed at eight scales using multiscale continuous wavelet transform (CWT), and distributed stochastic neighbor embedding (t-SNE) and decision tree (DT) models were used to select the optimal transformation scale. Among the four feature extraction methods, the combination of stacked autoencoders and Fisher score demonstrated the best performance for characterizing the spectral features of the treated chicken samples. Finally, <i>K</i>-nearest neighbors (KNNs), support vector machine (SVM), Light Gradient Boosting Machine (LightGBM), and DT were selected as classifiers to evaluate the overall performance. The results showed that the CWT-SAE-FisherScore-SVM model achieved the best classification performance (accuracy = 99.76%). Compared to benchmark methods such as PCA + SVM, this method demonstrates greater robustness and clearer physical significance at extremely low feature dimensions. This study established a proof-of-concept rapid screening framework for identifying simulated glucocorticoid residues in chicken in experimental settings. Although this model currently focuses on artificially spiked samples, it provides a robust technical foundation and an automatic segmentation algorithm to support the future development of real-time intelligent monitoring systems for practical food safety applications.</p>\u0000 </div>","PeriodicalId":15814,"journal":{"name":"Journal of Food Safety","volume":"46 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148324842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unusual Detection of Kurthia sp. Strain CMMC05 on ALOA Medium Leading to False-Positive Listeria Identification in Chicken Meat","authors":"Divya Suresh,&nbsp;Mohankumari Honganoor Puttananjaih,&nbsp;Mohan Appasaheb Dhale","doi":"10.1111/jfs.70084","DOIUrl":"https://doi.org/10.1111/jfs.70084","url":null,"abstract":"<div>\u0000 \u0000 <p>A bacterium was isolated during routine detection of <i>Listeria</i> spp. from chicken meat using Agar Listeria according to Ottaviani and Agosti (ALOA). The isolate produced bluish-green colonies on ALOA. This color indicates β-D-glucosidase activity, a feature commonly used to presumptively identify <i>Listeria</i> spp. However, later biochemical and molecular analysis revealed discrepancies compared with the reference strain <i>Listeria monocytogenes</i> ScottA. PCR analysis showed the absence of major virulence-associated internalin genes (<i>inlA, inlB,</i> and <i>inlC</i>). To confirm the taxonomic identity, 16S rRNA gene sequencing was performed. BLASTn analysis against the NCBI database identified the isolate as belonging to the genus <i>Kurthia</i>, with the highest sequence similarity to <i>Kurthia gibsonii</i> NBRC 15534^T. GC–MS fatty acid methyl ester analysis also revealed anteiso-C15:0 as the main fatty acid, matching <i>Kurthia</i> species. These results revealed that <i>Kurthia</i> sp. strain CMMC05 can grow on ALOA medium and resembles <i>Listeria</i> spp. Such results lead to false-positive identification. The study highlights a limitation of chromogenic media used in food safety testing and emphasizes the need for molecular identification to avoid misinterpretation of <i>Listeria</i> contamination in poultry products.</p>\u0000 </div>","PeriodicalId":15814,"journal":{"name":"Journal of Food Safety","volume":"46 3","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148282031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quadruplex Real-Time PCR for Rapid Detection and Prevalence Assessment of Vibrio parahaemolyticus, Vibrio vulnificus, Shigella spp., and Staphylococcus aureus in Aquatic Products","authors":"Jingjing Zou,&nbsp;Qinghua Ye,&nbsp;YuWei Wu,&nbsp;Jiahui Huang,&nbsp;Wangxia Liu,&nbsp;Langping Huang,&nbsp;Qiang Wan,&nbsp;Yanmei Feng,&nbsp;Zhihe Cai,&nbsp;Huishu Huang,&nbsp;Xinyu Zhao,&nbsp;Qingping Wu","doi":"10.1111/jfs.70083","DOIUrl":"https://doi.org/10.1111/jfs.70083","url":null,"abstract":"<div>\u0000 \u0000 <p>Surveillance of pathogenic bacteria in aquatic products is vital to public health. <i>Vibrio parahaemolyticus</i>, <i>Vibrio vulnificus</i>, <i>Shigella</i> spp., and <i>Staphylococcus aureus</i> represent important and complementary bacterial hazards associated with aquatic products, highlighting the need for rapid and simultaneous detection methods. This study aimed to develop a novel quadruplex real-time PCR system for detecting these four pathogens in aquatic products. Four sets of primers and TaqMan probes were newly designed, specific to the <i>pR72H</i>, <i>vvhA</i>, <i>ipaH</i>, and <i>kipA_2</i> genes, respectively. The assay exhibited high specificity against other common pathogens and no cross-reaction among these different bacteria. Analytical sensitivity ranged from 10⁻⁶ ng μL⁻¹ for <i>Shigella</i> spp. to 10⁻⁵ ng μL⁻¹ for <i>Vibrio</i> species and <i>Staphylococcus aureus</i>. The limits of detection in pure culture and spiked samples were 10¹ CFU mL⁻¹ for <i>Shigella</i> spp., 10² CFU mL⁻¹ for <i>Vibrio</i> species, and 10³ CFU mL⁻¹ for <i>Staphylococcus aureus</i>, respectively. To enhance the sensitivity to 10⁰ CFU mL⁻¹ directly from real samples, a 9-h pre-enrichment step was incorporated. The effectiveness of the method was validated using 365 diverse aquatic products from multiple coastal and inland regions in China, demonstrating diagnostic accuracy above 97.3% compared to traditional culture-based methods while offering a significant time reduction (3–7 days vs. 11 h). Overall pathogen prevalence across samples ranged from 0% to 21.4%. This study offers a practical tool for large-scale surveillance, enabling timely detection of key pathogens and supporting proactive food-safety interventions.</p>\u0000 </div>","PeriodicalId":15814,"journal":{"name":"Journal of Food Safety","volume":"46 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148238067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-thermal Plasma as a Decontamination Technology for Improving Microbial Safety of Fresh Produce","authors":"Marwa A. Kamel,&nbsp;Sherif Abd-Elmaksoud,&nbsp;Mohamed Ali Marouf,&nbsp;Mohamed Mokhtar Hefny,&nbsp;Mohamed Azab El-Liethy,&nbsp;Ibrahim Ahmed Hamza","doi":"10.1111/jfs.70082","DOIUrl":"https://doi.org/10.1111/jfs.70082","url":null,"abstract":"<div>\u0000 \u0000 <p>Nonthermal plasma (NTP) and plasma-activated water (PAW) are emerging nonthermal technologies for microbial decontamination of fresh vegetable and fruit products. Yet, their efficacy is strongly influenced by microbial type and matrix complexity. This study evaluated the antimicrobial performance of NTP and PAW against two foodborne bacterial pathogens, namely <i>Salmonella Typhimurium</i> (<i>S. Typhimurium</i>) and <i>Listeria monocytogenes</i> (<i>L. monocytogenes</i>), as well as bacteriophage ΦX174 as a viral surrogate. The effects of NTP and PAW were studied on two fresh produce surfaces of <i>Eruca sativa</i> (arugula) and Fragaria ananassa Duch (strawberry) in aqueous suspensions. The OES confirmed the generation of reactive oxygen and nitrogen species (ROS/RNS) with high electron density. In water, direct NTP achieved rapid inactivation of bacterial pathogens (&gt; 5 log₁₀ within 2–3 min), whereas efficacy on produce surfaces was reduced, with maximum reductions of ~2.5 log₁₀ on arugula and ~2 log₁₀ on strawberries, while higher reductions (&gt; 5 log₁₀) were observed in the corresponding aqueous phase. In contrast, PAW demonstrated strong bactericidal activity in aqueous systems (up to ~4.9 log₁₀) and on produce surfaces, achieving ~2.5 log₁₀ reduction on arugula and ~3.1 log₁₀ on strawberries for <i>S. Typhimurium</i>. However, viral inactivation was more limited and matrix-dependent, with PAW achieving ~2.3 log₁₀ reduction on arugula but only ~0.5 log₁₀ on strawberries. The scanning electron microscopy (SEM) revealed severe membrane damage to the selected bacterial pathogens after treatment and microbial localization within the produce surface pores, explaining matrix-associated protection. Overall, NTP and PAW show strong potential to enhance the safety of fresh produce, but further optimization is needed to overcome surface shielding and preserve product quality.</p>\u0000 </div>","PeriodicalId":15814,"journal":{"name":"Journal of Food Safety","volume":"46 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148174273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic and Molecular Docking Evaluation of Doxycycline Combined With Tannic, Salicylic, and Boric Acids Against Staphylococcus aureus","authors":"Karrar Al-Aklah,&nbsp;Somayeh Reiisi,&nbsp;Gharsrhasb Rigi,&nbsp;Razieh Heidari,&nbsp;Mehran Feizi-Dehnayebi","doi":"10.1111/jfs.70081","DOIUrl":"https://doi.org/10.1111/jfs.70081","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Staphylococcus aureus</i> is an important human pathogen with a high potential for antibiotic resistance and zoonotic transmission. Doxycycline is widely used in human and poultry infections, but its extensive application has accelerated the emergence of resistant strains. This study evaluated the potential of tannic acid, salicylic acid, and boric acid as adjuvants to doxycycline against <i>S. aureus</i> isolated from frozen chicken meat. Isolates were identified using selective culture media and biochemical tests. Antimicrobial activity was determined by broth microdilution assays to measure minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values. Synergistic interactions were assessed using the fractional inhibitory concentration index (FICI), while antibiofilm activity was also investigated. In addition, molecular docking analysis was performed using the target protein structure (PDB ID: 4URO). Doxycycline showed a MIC value of 0.5 μg/mL, whereas tannic acid, salicylic acid, and boric acid exhibited MIC values of 500, 1000, and 2000 μg/mL, respectively. Combination treatments reduced the MIC of doxycycline to 0.125 μg/mL with tannic acid and 0.25 μg/mL with salicylic acid, confirming synergistic effects, while boric acid showed no synergism. MBC and biofilm inhibition assays further supported the enhanced antibacterial activity of doxycycline combined with tannic acid or salicylic acid. Molecular docking results were consistent with the biological findings, showing the strongest binding affinity for doxycycline (−6.92 kcal/mol), followed by tannic acid (−5.61 kcal/mol) and salicylic acid (−5.49 kcal/mol), whereas boric acid showed weak binding (−3.22 kcal/mol). Overall, tannic acid and salicylic acid significantly enhanced the antimicrobial and antibiofilm effects of doxycycline against <i>S. aureus</i>, suggesting their potential as natural adjuvants for combating antibiotic resistance.</p>\u0000 </div>","PeriodicalId":15814,"journal":{"name":"Journal of Food Safety","volume":"46 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148173732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}