Journal of ethnopharmacology最新文献

{"title":"Phytochemical characterization, antioxidant and anti-inflammatory properties of Anethum graveolens L. seeds’ ethanolic extract: In vitro and In vivo studies","authors":"Nour El Houda Belabas ,&nbsp;Thoraya Guemmaz ,&nbsp;Haifaa Laroui ,&nbsp;Younes Douffa ,&nbsp;Nadjat Azzi ,&nbsp;Amel Barkat ,&nbsp;Karima Saffidine ,&nbsp;Fatima Zerargui ,&nbsp;Nadjat Righi ,&nbsp;Abderrahmane Baghiani","doi":"10.1016/j.jep.2025.120205","DOIUrl":"10.1016/j.jep.2025.120205","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div><em>Anethum graveolens</em> L. (Apiaceae), commonly known as \"zareaat el besbas\" in Algeria, is traditionally utilized in both traditional and modern Algerian medicine for its therapeutic properties.</div></div><div><h3>Aim of the study</h3><div>To analyze the chemical composition of the ethanolic crude extract (CrdE) from <em>A. graveolens</em> seeds and evaluate its antioxidant and anti-inflammatory activities.</div></div><div><h3>Materials and methods</h3><div>The CrdE was analyzed for its total polyphenol and flavonoid contents. High-performance liquid chromatography-diode array detector (HPLC-DAD) was used to identify the predominant bioactive compounds. <em>In vitro</em> antioxidant activities were evaluated using DPPH^•, OH^•, H₂O₂, and NO^• scavenging assays, lipid peroxidation inhibition, iron chelation, and ferric reducing power assays. Acute toxicity was assessed following the administration of CrdE at 5 g/kg<em>. In vivo</em> anti-inflammatory activity was tested using xylene-induced and carrageenan-induced edema models at a dose of 0.25, 0.5 and 1 g/kg.</div></div><div><h3>Results</h3><div>The CrdE contained significant levels of total polyphenols (26.33 ± 0.87 μg GAE/mg) and flavonoids (3.02 ± 0.03 μg QE/mg). HPLC-DAD analysis identified kaempferol (294.42 μg/g), chlorogenic acid (264.71 μg/g), and rutin (144.68 μg/g) as major bioactive compounds. The extract exhibited significant antioxidant potential across various assays, effectively scavenging free radicals, inhibiting lipid peroxidation, and demonstrating metal chelation and ferric reducing capabilities. The acute toxicity study revealed no adverse effects at the administered dose. <em>In vivo</em>, CrdE significantly reduced edema in xylene-induced (74 %) and carrageenan-induced (83.55 %) inflammation models.</div></div><div><h3>Conclusions</h3><div><em>A. graveolens</em> seeds are a rich source of bioactive compounds with notable antioxidant and anti-inflammatory properties, supporting their traditional medicinal use.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"352 ","pages":"Article 120205"},"PeriodicalIF":4.8,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144491307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eucalyptus torquata: Chemical profiling and the therapeutic potential of its buds extract against Toxoplasma gondii infection in mice","authors":"Enas F. Abdel Hamed ,&nbsp;Nelly M. Said ,&nbsp;Badreddine Drissi ,&nbsp;Ismail Mahdi ,&nbsp;Mohamed A.O. Abdelfattah ,&nbsp;Mansour Sobeh ,&nbsp;Mona F. Mahmoud","doi":"10.1016/j.jep.2025.120202","DOIUrl":"10.1016/j.jep.2025.120202","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div><em>Toxoplasma gondii</em> is a protozoan parasite that infects many warm-blooded animals, including humans. The genus <em>Eucalyptus</em> has been used for centuries, particularly in traditional medicine, for its antimicrobial and anti-inflammatory properties.</div></div><div><h3>Aim of the study</h3><div>The phytochemical composition of <em>Eucalyptus torquata</em> buds extract was profiled and its potential to mitigate toxoplasmosis-related symptoms, counter histopathological insults, and inhibit cyst development in a mice model were investigated.</div></div><div><h3>Materials and methods</h3><div>HPLC-MS/MS was used to identify and annotate the phytochemical contents. Protective effects against acute and chronic toxoplasma infection were evaluated. Several biochemical parameters were tested, including AST and ALT, along with histopathological and immunohistochemistry assays. Molecular docking was utilized to pinpoint the compounds associated with the anti-toxoplasmosis activity.</div></div><div><h3>Results</h3><div>HPLC-MS/MS analysis revealed that the plant extract contained 61 secondary metabolites belonging to various polyphenols and organic acids. Administration of <em>E. torquata</em> extract at 200 and 400 mg/kg/day for 14 consecutive days to mice initially infected with <em>T. gondii</em> ME-49 strain significantly prolonged the survival time compared to the untreated and spiramycin-treated groups. In addition, the extract, especially at 400 mg/kg, reduced the brain cyst burden (53 % and 75 %) and viability (38 % and 43 %), and decreased animals’ mortality rates in both acute (48^th-day post-infection) and chronic (64^th-day post-infection) phases. Monitoring liver and renal function revealed that the extract counteracted the infection-induced damage. It significantly reduced AST, ALT, and creatinine levels and increased the serum albumin level compared to the untreated mice. Furthermore, the extract-treated mice groups showed a substantial reduction in neuron degeneration, hepatic necrosis, and the associated inflammatory signs. Immunohistochemical analyses of the cytoplasmic inducible nitric oxide synthase (iNOS) in brain and liver tissues showed that both the extract and spiramycin elicited significant decreases in iNOS expression in acute and chronic phases. Molecular docking suggested that epigallocatechin-epigallocatechin, gallic acid coumaroyl gallate, gallic acid shikimate, and galloyl diglucose had the most favorable binding affinities towards iNOS enzyme.</div></div><div><h3>Conclusion</h3><div>Altogether, these findings open a promising avenue into the potential role of <em>E. torquata</em> phytochemicals in managing toxoplasmosis and the related complications.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"352 ","pages":"Article 120202"},"PeriodicalIF":4.8,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traditional knowledge and pharmacological evidence of pequi (Caryocar brasiliense) root bark for pain and inflammation","authors":"Ellen Pereira da Silva Maciel ,&nbsp;Katyuce Souza Farias ,&nbsp;Jéssica de Araújo Isaias Muller ,&nbsp;Iluska Senna Bonfá Moslaves ,&nbsp;Edson Lucas dos Santos ,&nbsp;Mônica Cristina Toffoli Kadri ,&nbsp;Ieda Maria Bortolotto ,&nbsp;Carlos Alexandre Carollo","doi":"10.1016/j.jep.2025.120203","DOIUrl":"10.1016/j.jep.2025.120203","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div><em>Caryocar brasiliense</em> (pequi) is used in Brazil for culinary and medicinal purposes. While its fruit and oil have been extensively studied, the root bark is traditionally used as an anti-inflammatory, but its pharmacological properties remain unexplored.</div></div><div><h3>Aim of the study</h3><div>This study investigates the chemical composition, antioxidant activity, anti-inflammatory and anti-hyperalgesic effects, and potential toxicity of <em>C. brasiliense</em> root bark to validate its traditional use.</div></div><div><h3>Materials and methods</h3><div>An aqueous extract was prepared via cold maceration (1–9 days) to simulate traditional use and assess compound stability. Chemical composition was analyzed by LC-DAD-MS, phenolics and tannins by the Folin-Ciocalteu method, and antioxidant activity via DPPH assay. Toxicity was evaluated in <em>Caenorhabditis elegans</em>. The anti-inflammatory and anti-hyperalgesic effects were tested in mice using carrageenan-induced paw edema and acetic acid-induced writhing.</div></div><div><h3>Results</h3><div>Throughout the 8-day maceration period, the extract maintained chemical stability, with an average phenolic content of 6.1 % and tannins at 5.0 %. It exhibited antioxidant activity (IC₅₀ = 16.15 ± 0.77 μg/mL). LC-DAD-MS identified galloyl tannins, ellagitannins, hydrolyzable tannins, and triterpenic saponins. <em>In vivo</em> assays confirmed anti-inflammatory and anti-hyperalgesic effects, with no toxicity in <em>C. elegans</em>.</div></div><div><h3>Conclusion</h3><div>The results validate the traditional use of <em>C. brasiliense</em> root bark for pain and inflammation, supporting its potential as a source of bioactive compounds with ethnopharmacological significance.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"352 ","pages":"Article 120203"},"PeriodicalIF":4.8,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144491235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiproliferative cassaine diterpenoid amines from the leaves and twigs of the traditional Thai medicinal plant Erythrophleum teysmannii (Kurz) Craib","authors":"Maria Orfanoudaki ,&nbsp;Dongdong Wang ,&nbsp;Lin Du ,&nbsp;Somsak Ruchirawat ,&nbsp;Prasat Kittakoop ,&nbsp;Tanja Grkovic ,&nbsp;Chulabhorn Mahidol ,&nbsp;Barry R. O'Keefe","doi":"10.1016/j.jep.2025.120167","DOIUrl":"10.1016/j.jep.2025.120167","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div><em>Erythrophleum teysmannii</em> (Kurz) Craib is a medicinal plant used by traditional healers in Laos and Thailand to treat cancer. Despite ethnopharmacological reports about plant extracts from this genus as cancer drug formulae, there is a knowledge gap about the chemical composition and potential pharmacological effects of <em>E. teysmannii</em>.</div></div><div><h3>Aim of the study</h3><div>The study aimed to investigate the phytochemical constituents of the leaf and twig DCM/MeOH extract of <em>E. teysmannii</em> and their antiproliferative properties, and to support its traditional uses.</div></div><div><h3>Materials and methods</h3><div>Bioassay-guided phytochemical investigation of the organic solvent extract of the plant led to the isolation of 21 pure compounds. Their structures were determined by extensive spectroscopic and spectrometric analyses, chemical derivatization and hydrolysis approaches, and ECD computational studies. Pure compounds were assessed for their antiproliferative activities against a panel of NCI-60 human tumor cell lines.</div></div><div><h3>Results</h3><div>Thirteen new cassaine diterpenoid amine monomers erythroteysamines A–M (<strong>1</strong>, <strong>4</strong>–<strong>9</strong>, <strong>11</strong>–<strong>14</strong>, <strong>16</strong>, and <strong>19</strong>) and two new dimers erythroteysamines N and O (<strong>20</strong> and <strong>21</strong>), together with six previously reported analogues (<strong>2</strong>, <strong>3</strong>, <strong>10</strong>, <strong>15</strong>, <strong>17</strong>, and <strong>18</strong>) were isolated. Sixteen compounds were tested in the NCI-60 assay, and they all averaged low micromolar antiproliferative potency.</div></div><div><h3>Conclusion</h3><div>The isolated compounds from <em>E. teysmannii</em> showed antiproliferative activities, providing chemical and pharmacological evidence supporting the traditional use of the plant in herbal medicine.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"352 ","pages":"Article 120167"},"PeriodicalIF":4.8,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144510733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tetrapleura tetraptera fruit (Schumach. & Thonn.) Taub. as a prospective anti-prostate cancer agent revealed through network pharmacology, molecular docking and dynamics, and in vitro studies","authors":"Joseph Kofi Abankwah ,&nbsp;Eunice Dotse ,&nbsp;Felix Boahen Owusu ,&nbsp;Hagnes Ofori-Attah ,&nbsp;Francis Bonsu Opoku ,&nbsp;Lidya Tesfaye Teka ,&nbsp;Eugene Ofori Asante ,&nbsp;Regina Appiah-Opong ,&nbsp;Alexander K. Nyarko","doi":"10.1016/j.jep.2025.120168","DOIUrl":"10.1016/j.jep.2025.120168","url":null,"abstract":"<div><h3>Ethnopharmacology relevance</h3><div>Cancer is a major global public health concern with significant ramifications on quality of life and treatment outcomes. Medicinal plants such as <em>Tetrapleura tetraptera</em> (TT) are key sources of bioactive compounds with therapeutic potential, yet their efficacy and underlying molecular mechanisms remain largely unexplored.</div></div><div><h3>Aim of the study</h3><div>To investigate the anti-prostate cancer potential, antioxidant activity, and underlying mechanism of TT.</div></div><div><h3>Materials and methods</h3><div>Crude extracts were obtained through the sequential extraction of powdered TT (whole fruit, pulp, and seed). TT extracts were assessed for their antioxidant activity, total phenolic content and antiprostate cancer activity using the DPPH radical scavenging assay, Folin-Ciocalteu method and tetrazolium-based colorimetric assay, respectively. Bioactive compounds from TT and prostate cancer-related targets were identified through literature mining and public databases. Network pharmacology, molecular docking and molecular dynamics were employed to elucidate TT's potential mechanisms of action.</div></div><div><h3>Results</h3><div>Aqueous whole fruit extract exhibited potent antioxidant activity with EC₅₀ = 83.46 ± 1.07 μg/mL, while the ethyl acetate fruit extract showed the strongest anti-prostate cancer activity (PC3 IC₅₀ = 5.39 ± 1.24 μg/mL, SI = 7.72; LNCaP IC₅₀ = 8.38 ± 1.27 μg/mL, SI = 4.97). Network pharmacology revealed that TT compounds targeted key proteins within the prostate cancer pathway, implicating MAPK, PI3K/AKT and P53 signaling cascades. Molecular docking and dynamics simulations further supported these findings, demonstrating strong, stable and flexible binding interactions between luteolin with MMP9/AR and Scopoletin with ERBB2.</div></div><div><h3>Conclusions</h3><div>Whole TT fruit demonstrates promising anti-prostate cancer activity through the multitargeted modulation of key proteins involved in pivotal signaling pathways. However, further <em>in vitro</em>, <em>in vivo</em> and clinical studies are required to validate these findings and fully explore TT's therapeutic potential.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"352 ","pages":"Article 120168"},"PeriodicalIF":4.8,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144491919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of glycolytic metabolic reprogramming and the TLR4/NF-κB/NLRP3 Pathway: comparative analysis of anti-inflammatory activity between two dosage forms of Pudilan xiaoyan","authors":"Ni Xu ,&nbsp;Xingru Lu ,&nbsp;Jingtao Yu ,&nbsp;Jiajun Gong ,&nbsp;Heng Guo ,&nbsp;Kaitong Chen ,&nbsp;Junshan Liu ,&nbsp;Jinying Ou ,&nbsp;Linzhong Yu ,&nbsp;Zibin Lu","doi":"10.1016/j.jep.2025.120204","DOIUrl":"10.1016/j.jep.2025.120204","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Ethnopharmacological relevance&lt;/h3&gt;&lt;div&gt;Pudilan xiaoyan oral liquid (PDL-L), a antipyretic and toxin-eliminating agent used in China for inflammatory conditions including sore throat and tonsillitis, is a standardized Chinese patent medicine. Pudilan formula granules (PDL-G), which share the same composition as PDL-L, are another dosage form of this medication. However, the difference in anti-inflammatory efficacy between PDL-L and PDL-G remains unevaluated, and their potential mechanisms require further exploration.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Aim of the study&lt;/h3&gt;&lt;div&gt;The present work systematically investigates comparative pharmacodynamic differences between PDL-L and PDL-G and their underlying mechanisms.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Materials and methods&lt;/h3&gt;&lt;div&gt;Qualitative analysis of key bioactive constituents in PDL-G was conducted using high performance liquid chromatography (HPLC) to ensure pharmaceutical quality standards. The anti-inflammatory effects of PDL-L and PDL-G were compared using three established models: the mouse auricular swelling model, the abdominal capillary permeability model, and the rat paw edema model. Additionally, to establish an inflammatory phenotype, zebrafish embryos at 3-day-post-fertilization received microinjection of 2-nL LPS solution (3 mg/mL) into the yolk sac region for inflammatory challenge. Comparative evaluation of anti-inflammatory efficacy between PDL-L and PDL-G were compared by larval survival analysis and observation of neutrophils and macrophages migration. Quantitative real-time PCR (qRT-PCR) was employed to measure transcriptional levels of pro-inflammatory cytokines and TLR4/NF-κB/NLRP3 pathway components in LPS-challenged zebrafish. The inhibitory effects of PDL-L and PDL-G on LPS- and Poly (I:C)-induced acute lung injury (ALI) were evaluated in mice through enzyme-linked immunosorbent assay (ELISA) and qRT-PCR. Pathological damage was evaluated &lt;em&gt;via&lt;/em&gt; hematoxylin-eosin (H&amp;E) staining. A concurrent evaluation was performed on glycolytic-related protein expression and TLR4/NF-κB/NLRP3 signaling activation in ALI mice.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;Qualitative profiling of eight principal constituents in PDL-G and PDL-L was achieved through simultaneous chromatographic detection. Both PDL-L and PDL-G exhibited remarkable anti-inflammatory effects in several types of inflammation models. Both two formulations reduced ear swelling, abdominal capillary permeability, and paw edema with no significant difference in efficacy. In zebrafish inflammation model, PDL-L and PDL-G both significantly suppressed neutrophils infiltration and macrophages accumulation in LPS-challenged zebrafish larvae, reduced the release of LPS-stimulated inflammatory factors, and prolonged larval survival through the TLR4/NF-κB pathway inhibition coupled with NLRP3 inflammasome suppression. In murine models of LPS/Poly (I:C)-induced ALI, both PDL-L and PDL-G effectively mitigated pulmonary inflammation through s","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"352 ","pages":"Article 120204"},"PeriodicalIF":4.8,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salvia officinalis L. leaf extracts: Chemical analysis and biological studies","authors":"Jessica Ceramella ,&nbsp;Daniel Chavarria ,&nbsp;Domenico Iacopetta ,&nbsp;Carlos Fernandes ,&nbsp;Maria Marra ,&nbsp;Antonio Gattuso ,&nbsp;Monica Rosa Loizzo ,&nbsp;Vincenzo Sicari ,&nbsp;Rosa Tundis ,&nbsp;Stefano Alcaro ,&nbsp;Fernanda Borges ,&nbsp;Maria Stefania Sinicropi","doi":"10.1016/j.jep.2025.120193","DOIUrl":"10.1016/j.jep.2025.120193","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div>Plants contain various bioactive molecules that may promote human health by preventing the onset and progression of different illnesses, including cancer, diabetes, neurodegenerative conditions, and cardiovascular issues. <em>Salvia species</em> have been employed since ancient times in traditional medicine and for culinary use.</div></div><div><h3>Aim of the study</h3><div>Herein, four extracts from leaves of <em>Salvia officinalis</em> L., cultivated in Calabria (Italy) were obtained and quali- and quantitatively characterized, finding a high presence of bioactive compounds. The extracts were investigated for their biological activities, showing interesting antioxidant, anti-inflammatory and anticancer properties. In addition, all the extracts were tested for their potential regulation of some enzymes involved in neurological and neurodegenerative diseases, as MAO-A and B, AChE and BChE. Finally, the safety of the extracts was also investigated.</div></div><div><h3>Materials and methods</h3><div>The extracts were obtained using conventional maceration and ultrasound-assisted extractions. The chemical characterization was achieved by the means of Ultra-High Performance Liquid Chromatography. The biological evaluation was performed by <em>in vitro</em>, direct enzymatic, fluorescence and cell-based assays.</div></div><div><h3>Results</h3><div>The chromatographic analysis indicated a high presence of bioactive compounds, which confer high ability in ROS scavenging, NO production inhibition and impacting breast cancer cells viability. In addition, all the extracts targeted some enzymes involved in neurological and neurodegenerative diseases, as MAO-A and B, AChE and BChE. Moreover, the extracts were found safe and with a low hepatotoxic toxicity.</div></div><div><h3>Conclusions</h3><div>The present study demonstrated that the extracts from <em>Salvia officinalis</em> L. leaf, traditionally used for many puproses, possess various biological activities, regulating the oxidative stress and inflammation, reducing the growth of breast cancer cells and blocking some key enzymes involved in neurological diseases. The combined low toxicity and biological features reported in this work suggest a high potential of the studied extracts for the management of some pathological conditions and/or for the achievement of nutraceutical products.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"352 ","pages":"Article 120193"},"PeriodicalIF":4.8,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calycosin limits hepatic fibrogenesis via KLF15 dependent repression of P2X7R in thioacetamide-induced hepatic fibrosis","authors":"Jin-Jin Zhang ,&nbsp;Wan-Ling Wang ,&nbsp;Jiang-Tao Zhong ,&nbsp;Hai-Ming Sun ,&nbsp;Yu-Chen Jiang ,&nbsp;Yue Shang ,&nbsp;Jian Song","doi":"10.1016/j.jep.2025.120194","DOIUrl":"10.1016/j.jep.2025.120194","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div><em>Astragalus membranaceus (Fisch.) Bunge</em> is a traditional Chinese medicinal herb that has been documented for its hepatoprotective properties and therapeutic potential. Calycosin, derived from <em>Astragalus membranaceus (Fisch.) Bunge</em> is a natural flavonoid widely used in ameliorating chronic liver diseases.</div></div><div><h3>Aim of the study</h3><div>The current study investigates hepatoprotective effects as well as the underlying mechanism of Calycosin on hepatic fibrosis.</div></div><div><h3>Materials and methods</h3><div>Hepatic fibrosis was induced in mice (n = 6) by intraperitoneal injection of thioacetamide (TAA). Subsequently, we deduced the hepatic fibrosis signaling network using RNA sequencing. LX-2 and mouse primary hepatocytes were transfected with the siRNA-KLF15; mouse bone marrow-derived macrophages (BMDMs) were also treated with LPS/ATP and Calycosin to produce conditioned medium. Dual luciferase assay characterized KLF15-P2X7r promoter interaction.</div></div><div><h3>Results</h3><div>RNA sequencing showed that the KLF15/NOD-like receptor pathway played a significant role in the Calycosin-mediated hepatoprotection in TAA-induced mouse models. Calycosin treatment resulted in a 33 % reduction in ALT and a 35 % decrease in AST levels. In TAA-induced mouse models of hepatic fibrosis, Calycosin greatly diminished the formation of neutrophil extracellular traps. Calycosin treatment induced KLF15 upregulation and a 3-fold reduction in P2X7R protein expression. In the hepatocyte and LX-2, KLF15 deletion cancels the inhibitory effect of Calycosin on the P2X7r. It is shown that KLF15 directly binds to the P2X7r promoter. Furthermore, Calycosin blocked pyroptosis in BMDMs, especially via Calycosin interaction with HSCs-macrophage crosstalk.</div></div><div><h3>Conclusions</h3><div>This study implies that Calycosin alleviates hepatic fibrosis in the fibroinflammatory lesion through modulating the KLF15-P2X7r/NLRP3 signaling pathway.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"352 ","pages":"Article 120194"},"PeriodicalIF":4.8,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms and synergistic effects of the active components of Xanthocerais lignum in inhibiting rheumatoid arthritis through the modulation of the biological behavior of synovial cells.","authors":"Hao Qian, Cuilan Bai, Xin Jia, Yaqiong Yang, Xiangyang Tian, Xiaoqin Wang","doi":"10.1016/j.jep.2025.120200","DOIUrl":"https://doi.org/10.1016/j.jep.2025.120200","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Ethnopharmacological relevance: &lt;/strong&gt;Xanthocerais lignum, a classic medicinal herb in Mongolian medicine for the treatment of rheumatoid arthritis (RA), is documented in traditional Mongolian texts such as the \"Wu Wu Meng Yao Jian\" and \"Meng Yao Zhi\" for its efficacy in clearing heat, reducing swelling, and modulating \"Xie Ri Wu Su\" (the pathogenesis related to rheumatism). However, the active compounds and molecular mechanisms of action remain inadequately elucidated, hindering its modernization and development.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Aim of the study: &lt;/strong&gt;This study aims to systematically elucidate the molecular mechanisms by which the active components of Xanthocerais lignum inhibit RA through the modulation of the biological behavior of synovial cells, while also revealing the patterns of synergistic interactions among its multiple components.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Materials and methods: &lt;/strong&gt;Initially, serum network pharmacology was utilized to predict the potential active components and mechanisms of Xanthocerais lignum against RA, followed by quantitative analysis of 6 primary active ingredients via high performance liquid chromatography (HPLC). Subsequently, a collagen-induced arthritis (CIA) rat model was established, and the expression of relevant proteins and mRNA in synovial tissues was assessed using western blot and quantitative real-time polymerase chain reaction (qPCR). Primary fibroblast-like synoviocytes (FLS) were isolated and cultured using the tissue block adherence culture method. Cell proliferation, invasion, apoptosis, and other assays were conducted to evaluate the biological effects of the active components and explore their mechanisms. Finally, the median drug-effect analysis (Chou-Talalay method) and molecular simulation were employed to investigate the synergistic effects and mechanisms among the active components.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Serum network pharmacology predictions indicated that Xanthocerais lignum may exert its anti-RA effects by regulating biological processes such as cell proliferation, apoptosis, and inflammation via the PI3K-Akt signaling pathway. Animal experiments confirmed that the ethanol extract and ethyl acetate fraction of Xanthocerais lignum significantly reduced the abnormal overexpression of key proteins like phosphoinositide 3-kinase (PI3K) and protein kinase B (AKT) in the synovial tissues of CIA rats. Further in vitro experiments revealed that the content of the 6 active components in Xanthocerais lignum exceeded 1.4 mg/g, and they were found to modulate various biological processes in primary RA-FLS cells, including proliferation, invasion, migration, apoptosis, cycle, and inflammation, alongside the expression of the PI3K-Akt signaling pathway. It is noteworthy that quantitative analysis using the median drug-effect method revealed that the combined administration of epicatechin and procyanidin A2 exerted a markedly synergistic inhibitory effect on RA-FLS cell proliferat","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":" ","pages":"120200"},"PeriodicalIF":4.8,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Astragulus embranaceus (Fisch.) Bge-Dioscorea opposita Thunb herb pair ameliorates sarcopenia in senile type 2 diabetes mellitus through Rab5a/mTOR-mediated mitochondrial dysfunction\" [J. Ethnopharmacol. 317 (2023) 116737].","authors":"Meiling She, Minna Huang, Jing Zhang, Yan Yan, Lingli Zhou, Meng Zhang, Yajun Yang, Dongtao Wang","doi":"10.1016/j.jep.2025.120180","DOIUrl":"https://doi.org/10.1016/j.jep.2025.120180","url":null,"abstract":"","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":" ","pages":"120180"},"PeriodicalIF":4.8,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144497199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}