Journal of ethnopharmacology最新文献

{"title":"Phytochemical analysis and wound healing properties of Malva parviflora L. ethanolic extract","authors":"","doi":"10.1016/j.jep.2024.118983","DOIUrl":"10.1016/j.jep.2024.118983","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div>Scientific publications documented the use of plants from Genus <em>Malva</em> to treat inflammatory diseases and skin disorders by our ancestors. <em>Malva parviflora</em> L. has reported benefits for wound healing in traditional medicine; however, there is a lack of experimental study to validate these claims.</div></div><div><h3>Aim</h3><div>We initiated this study to explore the metabolites and verify the wound healing properties of <em>M. parviflora</em> using <em>in vivo</em> and <em>in vitro</em> models.</div></div><div><h3>Materials and methods</h3><div>Liquid chromatography electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) was used to identify the ethanolic extract different metabolites. Additionally, total phenolic content was determined using Folin-Ciocalteu reagent. To verify the extract wound healing potential, an <em>in vivo</em> rat wound excision model was employed. Round wounds (5 mm in diameter) were created by a sterile biopsy punch needle. The wounds were treated with plant extracts (2.5% and 5%) as well as a commercially available wound healing product (Mebo®) for 10 days. The results were assessed as follows: 1) Measuring the reduction% in wound area compared to the original wound size. 2) Evaluation of the levels of wound healing biomarkers, namely collagen type I (Col-1), alpha smooth muscle actin (<em>α</em>-SMA), extracellular signal-regulated kinases-1 (ERK1), and matrix metalloproteinase-9 (MMP9) levels. 3) Performing histopathological examination of the wound tissue. The antioxidant properties of the <em>M. parviflora</em> leaves ethanolic extract were investigated using various assays: total antioxidant capacity (TAC), iron reducing power (IRP), 1,1-Diphenyl-2-picryl-hydrazyl (DPPH), and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals scavenging assays. Furthermore, the anti-inflammatory activity was confirmed by calculating the inhibition percentages of protein denaturation and the activity of the proteinase enzyme.</div></div><div><h3>Results</h3><div>Liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis revealed the presence of various secondary metabolites in <em>M. parviflora</em> ethanolic extract, including phenolic acids (cinnamic and ferulic acids), flavonoids (quercetin and “iso”rhamnetin monoglucuronides), fatty acids (hydroxy-octadecatrienoic and oxo-octadecatrienoic acids), in addition to chlorophyll derivatives and carotenoids (pheophorbide-a and lutein, respectively). <em>Malva</em> extracts significantly reduced wound size compared to untreated control group. The extracts also promoted wound healing by upregulating collagen I, <em>α</em>-SMA, and ERK1 levels, while downregulating MMP9 expression. Notably, the effect of 2.5% and 5% extracts was similar or exceeds those of Mebo®, supported by histopathological results. Finally, <em>M. parviflora</em> ethanolic extract exhibited antioxidant and anti-inflamm","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xihuang pill suppresses breast cancer malignancy by inhibiting TGF-β signaling and acquires chemotherapy benefits","authors":"","doi":"10.1016/j.jep.2024.119000","DOIUrl":"10.1016/j.jep.2024.119000","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div>Breast cancer (BC) has an extremely high global incidence rate. The Xihuang pill (XHP), a traditional Chinese formula, originates from Hongxu Wang's “Life-Saving Manual of Diagnosis and Treatment of External Diseases” written during the Qing Dynasty. In this book, XHP, was first suggested as an anticancer treatment for BC. However, the regulatory mechanism of XHP on BC requires further investigated.</div></div><div><h3>Aim of the study</h3><div>To assess the effects of XHP on BC and elucidate the underlying associated signaling network.</div></div><div><h3>Materials and methods</h3><div>The influence of XHP on cellular viability, proliferation, and apoptosis of MDA-MB-231 and BT-549 cells were examined. The ability to metastasize was evaluated using Transwell invasion and wound healing tests. Western blotting was used to examine the epithelial-mesenchymal transition (EMT) markers expression. RNA sequencing and bioinformatic analysis were utilized to investigate the regulation mechanism of XHP. A subcutaneous tumor model was developed to study the tumor-inhibitory effects of XHP or/and Doxorubicin (Dox) on BALB/c nude mice, and the EMT marker levels in tumor tissues were determined using immunohistochemical labeling.</div></div><div><h3>Results</h3><div>XHP demonstrated anticancer effects on BC cells by suppressing cell proliferation, inducing cellular apoptosis, and inhibiting EMT progression. XHP may regulate the EMT via the TGF-β axis, as shown by RNA sequencing and Western blotting analysis. Furthermore, the combination of XHP and Dox had a stronger therapeutic effect on BC cell proliferation, apoptosis, and metastasis in both cellular and animal models.</div></div><div><h3>Conclusions</h3><div>We were the first to reveal that XHP abrogated EMT progression via modulating the TGF-β axis. Furthermore, the combination therapy of XHP and Dox presents a promising novel therapeutic candidate for BC patients.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scutellarein inhibits lung cancer growth by inducing cell apoptosis and inhibiting glutamine metabolic pathway","authors":"","doi":"10.1016/j.jep.2024.118999","DOIUrl":"10.1016/j.jep.2024.118999","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div>Scutellaria baicalensis Georgi, a widely used Chinese medicinal herb, has shown effectiveness against lung cancer. Scutellarein, a key component of <em>Scutellaria baicalensis</em>, also demonstrates anticancer properties in lung cancer. However, the underlying mechanisms have not yet been clarified.</div></div><div><h3>Aim of the study</h3><div>This study aimed to investigate the effects of scutellarein in the treatment of NSCLC and its underlying mechanisms.</div></div><div><h3>Methods</h3><div>This study explored the effects of scutellarein on non–small cell lung cancer (NSCLC) and its mechanisms. A Lewis lung cancer mouse model was established to assess scutellarein's anticancer activity <em>in vivo</em>. Additionally, the compound's effects on cell proliferation, colony formation, migration, and apoptosis were evaluated <em>in vitro</em> using A549 and H1299 lung cancer cells. Metabolomics analysis was conducted to identify changes in cellular metabolism due to scutellarein, while molecular docking and western blotting techniques were employed to elucidate the molecular mechanisms of its anti-lung cancer effects.</div></div><div><h3>Results</h3><div>Scutellarein significantly inhibited lung cancer xenograft tumor growth. <em>In vitro</em> studies showed that scutellarein suppressed migration and colony formation in A549 and H1299 cells, induced cell cycle arrest, and triggered cell apoptosis. Notably, scutellarein profoundly altered amino acid metabolism, particularly affecting glutamine metabolites. It affected key glutamine transporters ASCT2 and LAT1, as well as glutaminase GLS1, leading to their reduced expression.</div></div><div><h3>Conclusion</h3><div>Scutellarein effectively inhibits lung cancer growth both <em>in vivo</em> and <em>in vitro</em> by inducing cell apoptosis and downregulating the glutamine metabolic pathway.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo evaluation of anti-diarrheal activity of hydroalcoholic extract and solvent fractions of the leaf of Leucas deflexa Hook.f (Lamiaceae) in rodents","authors":"","doi":"10.1016/j.jep.2024.118993","DOIUrl":"10.1016/j.jep.2024.118993","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div>The emergence of antibiotic-resistant bacteria has intensified the search for novel antidiarrheal drug. Plant-derived extracts offer promising alternatives due to their ability to modulate gut motility and enhance water absorption. The leaves of <em>Leucas deflexa</em> Hook.f., a plant native to Ethiopia, have been traditionally utilized in the treatment of diarrheal diseases. Nonetheless, its traditional application has yet to be scientifically confirmed.</div></div><div><h3>Aim of the study</h3><div>Hence, the aim of the present study was to evaluate antidiarrheal activity of the crude extract and fractions of the leaf of <em>Leucas deflexa</em> Hook.f (Lamiaceae).</div></div><div><h3>Materials and methods</h3><div>Healthy Swiss albino mice were randomly divided into five groups per experimental model: castor oil-induced diarrhea, gastrointestinal motility, and anti-enteropooling assays. The control group received distilled water (10 ml/kg), while the positive control was treated with loperamide (3 mg/kg). The remaining groups were administered various dosages (LD100, LD200, and LD400 mg/kg) of a hydroalcoholic extract or its fractions from <em>Leucas deflexa</em> Hook.f.</div></div><div><h3>Result</h3><div>The crude extract and chloroform fraction substantially (P &lt; 0.001) delayed the onset of diarrhea, reduced fecal frequency, and decreased total fecal weight at all administered doses compared to the negative control. The butanol and aqueous fractions exhibited a substantial antidiarrheal effect at doses of 200 mg/kg and 400 mg/kg, respectively. All treatments, including the crude extract, chloroform, butanol, and aqueous fractions, substantially (P &lt; 0.001) reduced the volume and weight of intestinal contents at various doses. Additionally, the chloroform, aqueous fraction, and crude extract at doses of 100, 200, and 400 mg/kg substantially inhibited gastrointestinal motility compared to the negative control.</div></div><div><h3>Conclusion</h3><div>The results of this study corroborate the traditional use of <em>Leucas deflexa</em> leaves in the treatment of diarrhea. These findings give a scientific justification for further exploration of the underlying mechanisms and potential therapeutic applications of this plant extract.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uncaria rhynchophylla alkaloid extract exerts neuroprotective activity against Parkinson's disease via activating mitophagy with the involvement of UCHL1","authors":"","doi":"10.1016/j.jep.2024.119009","DOIUrl":"10.1016/j.jep.2024.119009","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div><em>Uncaria rhynchophylla</em> (Miq.) Miq. ex Havil. (UR), a traditional Chinese medicinal plant, plays an active role in neuroprotection. Clinical medication and modern pharmacological studies have proved the efficacy of UR against Parkinson's disease (PD), with alkaloids being recognized as the main bioactive components. But the therapeutic effect and mechanism of <em>U. rhynchophylla</em> alkaloid extract (URA) against PD need further exploration.</div></div><div><h3>Aim of study</h3><div>The study aimed to investigate the therapeutic effect and potential mechanism of URA on PD.</div></div><div><h3>Materials and methods</h3><div>LC-MS methodology was used to evaluate the chemical constituents of URA. The anti-PD activity of URA in vivo was measured on the mouse and rat models of PD. Neuroprotective effect of URA on PC12 cells was measured by MTT assay. Dopamine (DA) and its metabolites were detected by LC-MS for probing the protection ability on dopaminergic neurons. The differentially expressed proteins between model group and URA administrated group were analyzed by proteomics, suggesting oxidative phosphorylation as possible pathway of URA. Considering the critical role of mitochondria in oxidative phosphorylation, JC-1 staining, MitoSOX staining, transmission electron microscopy (TEM) observation and adenosine triphosphate (ATP) levels detection were used to analyze the effects of URA on mitochondrial morphology and function. Biolayer interferometry (BLI) was used to search for the possible UCHL1-bonding compounds in URA.</div></div><div><h3>Results</h3><div>URA significantly mitigated the behavioural defects by improving coordination, shortening the time to climb down the whole pole (T-LA) and increasing the forelimbs’ muscle strength of MPTP-induced PD mice and 6-OHDA-induced PD rats. In addition, URA improved tyrosine hydroxylase expression in dopaminergic neurons by immunohistochemistry (IHC) staining, and thus increased the neurotransmitter levels of DA and relevant metabolites. Furthermore, URA promoted mitophagy as reflected by a significant decrease in reactive oxygen species (ROS) generation, an increase in ATP levels and clearance of damaged mitochondria. Subsequently, Ubiquitin C-terminal hydrolase 1 (UCHL1), which is associated with the mitochondrial dysfunction in PD, is suggested to be a promising target based on the proteomics result, and proved by the blocked protective effects of URA by UCHL1 inhibitor. Furthermore, hirsuteine (HTE) was identified as a potential active compound of URA binding to UCHL1 by BLI, and the binding capacity and site were verified by surface plasmon resonance (SPR) and molecular docking.</div></div><div><h3>Conclusion</h3><div>This work demonstrates that URA exerts effective neuroprotective activity against PD via activation of mitophagy with the involvement of UCHL1, and HTE may be a potential active compound of URA.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traditional Chinese Medicine formulas-based interventions on colorectal carcinoma prevention: The efficacies, mechanisms and advantages","authors":"","doi":"10.1016/j.jep.2024.119008","DOIUrl":"10.1016/j.jep.2024.119008","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div>The Traditional Chinese Medicine Formulas (TCMFs) represent a distinctive medical approach to disease treatment and have been utilized in clinical practice for treating intestinal diseases for thousands of years. Recently, TCMFs have received increasing attention due to their advantages of high efficiency, safety, as well as low toxicity, providing promising strategies for preventing colorectal carcinoma (CRC). Nonetheless, the potential mechanism of TCMFs in preventing CRC has not been fully elucidated.</div></div><div><h3>Aim of the study</h3><div>The literature from the past three years was reviewed to highlight the therapeutic effects and underlying mechanisms of TCMFs in preventing CRC.</div></div><div><h3>Materials and methods</h3><div>The keywords have been searched, including “traditional Chinese medicine formulas,” “herb pairs,” “Herbal plant-derived nanoparticles,” et al. in “PubMed” and “China National Knowledge Infrastructure (CNKI),” and screened published articles related to the treatment of intestinal precancerous lesions. This review primarily examined the effectiveness and mechanisms of TCMFs in treating intestinal precancerous lesions, highlighting their significant potential in preventing CRC.</div></div><div><h3>Results</h3><div>Gegen Qinlian decoction, Shaoyao decoction, Wu Wei Wan, etc., exert substantial therapeutic effects on intestinal precancerous lesions. These therapeutic effects are demonstrated by a reduction in disease activity index scores, suppression of intestinal inflammation, and preservation of body weight and intestinal function, all of which contribute to the effective prevention of CRC. Besides, the classic Chinese herbal pairs and the extracellular vesicle-like nanoparticles of herbaceous plants have demonstrated superior efficacy in the treatment of intestinal precancerous lesions. Mechanistically, protecting the epithelial barrier, regulating gut microbiota as well as related metabolism, modulating macrophage polarization, and maintaining immune balance contribute to the role of TCMFs in CRC prevention.</div></div><div><h3>Conclusions</h3><div>This review demonstrates the great potential and mechanism of TCMFs in CRC prevention and provides a scientific basis for their utilization in CRC prevention.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect and mechanism of Magnolia officinalis in colorectal cancer: multi-component-multi-target approach.","authors":"He Zhang, Jun Tang, Huiliang Cao, Chenguang Wang, Chong Shen, Jun Liu","doi":"10.1016/j.jep.2024.119007","DOIUrl":"https://doi.org/10.1016/j.jep.2024.119007","url":null,"abstract":"<p><strong>Ethnopharmacological relevance: </strong>Colorectal cancer (CRC) is a prevalent malignant tumor of the digestive tract. Traditional Chinese medicine (TCM) has a long history of treating CRC, with advantages such as effectiveness, multi-target, multi-pathway, and minimal side effects. TCM Magnolia officinalis (M. officinalis) refers to the dried bark, root bark, and branch bark of either Magnolia officinalis Rehd.et Wils. or Magnolia officinalis Rehd.et Wils. var. biloba Rehd.et Wils.. It is commonly utilized to alleviate the side effects of chemotherapy for CRC, owing to its anti-inflammatory and anti-tumor properties. However, current research primarily focuses on the individual components and does not take into consideration the characteristics of multi-component-multi-target action.</p><p><strong>Aim of the study: </strong>Our aim is to study the new action characteristics of M. officinalis in the treatment of CRC.</p><p><strong>Materials and methods: </strong>Utilizing network pharmacology to identify potential active ingredients, key targets, and main signaling pathways of M. officinalis for the treatment of CRC. The binding effect was further validated through molecular docking analysis. Furthermore, the aforementioned components were identified using liquid chromatography-mass spectrometry (LC-MS), and the cleavage pathways of the main components were analyzed. Subsequently, both in vitro and in vivo experiments were carried out to investigate the anti-CRC effect of the active ingredients of M. officinalis and its potential mechanism.</p><p><strong>Results: </strong>Network pharmacology and Molecular docking identified 5 main active ingredients and 6 core targets of M. officinalis for the treatment of CRC. Then, LC-MS identified the active components of M. officinalis. At the same time, both in vitro and in vivo experiments have confirmed the ability of Eucalyptol (Euc) and Obovatol (Obo)to inhibit inflammation and tumor cell proliferation. The possible mechanism involved is that Euc and Obo counteract CRC by inhibiting the over-activation of NF-κBp65/JAK and Bcl-2/Caspase signaling pathways, respectively. They also play a role in the anti-CRC effect of M. officinalis.</p><p><strong>Conclusion: </strong>Magnolol (MAG), Honokiol (HK), Euc, Obo, and Neohesperidin (NHP) in M. officinalis may be the pharmacological substance basis for its anti-cancer effect on CRC. The treatment of CRC with M. officinalis is characterized by its multi-component, multi-target, and multi-pathway approach. These findings provide a theoretical basis for further inspiring the clinical application of M. officinalis and the development of efficacy targets.</p>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leech granules inhibit ferroptosis and alleviate renal injury in mice with diabetic kidney disease","authors":"","doi":"10.1016/j.jep.2024.118995","DOIUrl":"10.1016/j.jep.2024.118995","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div>The underlying mechanisms of diabetic kidney disease (DKD) and effective treatment strategies remain unclear. DKD progression is closely associated with abnormal iron metabolism and ferroptosis <em>in vivo</em>. Leeches, used in traditional Chinese medicine for promoting blood circulation and resolving blood stasis, are utilized to treat diabetes and its associated complications. Leeches effectively antagonize oxidative stress injury and exert protective effects on renal function. However, whether leeches can inhibit ferroptosis by modulating oxidative stress and iron metabolism remains unclear.</div></div><div><h3>Aim of the study</h3><div>To investigate the therapeutic potential of leech granules in DKD and, specifically, their effects on ferroptosis.</div></div><div><h3>Materials and methods</h3><div>We used a mouse model of DKD. The mice were treated with leech granules via gavage. Component identification and analysis of leech granules were performed using UPLC-MS, and efficacy was assessed by histopathology and analysis of blood glucose, lipids, and renal function. Additionally, the pharmacological mechanisms of leech granules were explored via proteomics, immunohistochemical staining, western blotting, and cell culture.</div></div><div><h3>Results</h3><div>Proteomic analysis showed that iron metabolism was dysregulated and ferroptosis increased in DKD mice. Leech granules significantly reduced iron accumulation and renal pathological damage, decreased ROS levels, upregulated GSH levels, and inhibited ferroptosis in the kidneys of DKD mice. Furthermore, <em>in vitro</em> cellular experiments demonstrated that leech granules could inhibit erastin-induced ferroptosis and protect renal cells.</div></div><div><h3>Conclusions</h3><div>The regulation of renal iron metabolism and inhibition of ferroptosis mediates the therapeutic effect of leech granules on DKD. Leech granules represent a promising approach for DKD treatment.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ammopiptanthus nanus (M. Pop. ) Cheng f. stem ethanolic extract ameliorates rheumatoid arthritis by inhibiting PI3K/AKT/NF-κB pathway-mediated macrophage infiltration.","authors":"Yuan Yao, Jiaye Wang, Hongjuan Zhang, Tao Peng, Yanpei Sun, Ruili Zhang, Xiang Meng, Xu Lu, Yankun Gao, Yang Jin, Yu Zhang, Lina Chen","doi":"10.1016/j.jep.2024.118974","DOIUrl":"https://doi.org/10.1016/j.jep.2024.118974","url":null,"abstract":"<p><strong>Ethnopharmacological relevance: </strong>Ammopiptanthus nanus (M. Pop. ) Cheng f. (A. nanus), a traditional Kirgiz medicinal plant, its stem has shown potential in treating rheumatoid arthritis (RA) in China, either through oral medication or by topical application directly to the affected joints, but its underlying mechanism of action remains unexplored.</p><p><strong>Aim of the study: </strong>The purpose of this study is to elucidate pharmacological mechanism of A. nanus in ameliorating RA using a comprehensive approach that combines network pharmacology, molecular docking and experimental evaluations.</p><p><strong>Materials and methods: </strong>Firstly, the major constituents of A. nanus stem ethanolic extract were identified and quantified by High-Performance Liquid Chromatography (HPLC). Disease target data from Gene Cards database was then used to define RA-associated targets. A protein-protein interaction (PPI) network was created via STRING database. The DAVID database powered gene ontology (GO) function and kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis to gain functional insights. In vitro, RAW264.7 cells were treated with A. nanus to investigate the roles of target proteins and pathways during lipopolysaccharide (LPS) - induced inflammation. Immunofluorescence assays were performed to assess the effects of A. nanus on macrophage infiltration. The key targets and signalling pathways were validated using enzyme-linked immunosorbent assay (ELISA), real-time quantitative polymerase chain reaction (RT-qPCR), molecular docking, immunohistochemical analysis, western blotting and immunofluorescence. Finally, the therapeutic potential of A. nanus in RA was evaluated in a carrageenan-induced rat model.</p><p><strong>Results: </strong>Network analysis identified 31 potential targets of A. nanus associated with RA, including 10 hub targets. KEGG analysis highlighted the involvement of PI3K/AKT signaling pathway. In vivo experiments demonstrated that A. nanus treatment significantly protected against carrageenan-induced inflammatory paw tissue and attenuated macrophage infiltration. Both in vivo and in vitro experiments confirmed that A. nanus significantly downregulated the protein expression of COX-2, iNOS and IL-1β, and inhibited PI3K/AKT/NFκB pathway, which are closely linked to RA. Furthermore, molecular docking and cellular thermal shift assay revealed that licoflavanone showed a strong binding affinity with key targets.</p><p><strong>Conclusion: </strong>In summary, this study provides the first evidence of the potent anti-inflammatory activity of A. nanus in experimental RA. The mechanism of action appears to involve inactivation of the PI3K/AKT/NF-κB pathway-mediated macrophage infiltration. These findings indicate that A. nanus has significant potential as a therapeutic potential agent for RA treatment and offer novel insights for future research and drug development in this field.</p>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical analysis and evaluation of the inhibitory effect of the Cunila lythrifolia Benth aerial parts on abdominal pain and some digestive enzymes","authors":"","doi":"10.1016/j.jep.2024.118991","DOIUrl":"10.1016/j.jep.2024.118991","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Ethnopharmacology relevance&lt;/h3&gt;&lt;div&gt;&lt;em&gt;Cunila lythrifolia&lt;/em&gt; Benth. (Lamiaceae) commonly named “poleo de monte” is a Mexican plant known since pre-Hispanic times because of the properties of its aerial parts to treat respiratory and gastrointestinal conditions, for postpartum care, and as an aphrodisiac.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Aim of the study&lt;/h3&gt;&lt;div&gt;To obtain preclinical evidence of the pharmacological properties of &lt;em&gt;C. lythrifolia&lt;/em&gt; on abdominal pain and some digestive enzymes, as well as its chemical composition.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Material and methods&lt;/h3&gt;&lt;div&gt;The preclinical safety of a decoction was evaluated by the Lorke method in mice. The antinociceptive effect was assessed using the acetic acid-induced writhing test in mice, and the mechanism of action was explored by the co-administration of naloxone. Additionally, the inhibition of the lipase and α-amylase was carried out using a colorimetric assay to calculate the percentage of inhibition. The isolation of specialized metabolites was carried out using chromatographic techniques, and characterization was established with MS, NMR, and chiroptical analysis. The volatile components of the aerial parts were identified by GC–MS analysis of the essential oils, and by HSPM coupled with GC−MS. An analytical method by UHPLC was validated under the guidelines of the ICH, for the quantification of &lt;strong&gt;1&lt;/strong&gt;.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;The LD&lt;sub&gt;50&lt;/sub&gt; of the aqueous extract is higher than 5 g/kg. The decoction and the essential oil have an antinociceptive effect at 100 and 10 mg/kg, respectively. The essential oil was active against the lipase enzyme (96−76 % of inhibition). The isolated compounds from the decoction were linarin (&lt;strong&gt;1&lt;/strong&gt;), 7-&lt;em&gt;O&lt;/em&gt;-[2-&lt;em&gt;O&lt;/em&gt;-acetyl-β-D-glucopyranosyl-(1 → 2)-[α-L-rhamnopyranosyl-(1 → 6)]-β-D-glucopyranoside] (&lt;strong&gt;2&lt;/strong&gt;), lithospermic acid (&lt;strong&gt;3&lt;/strong&gt;), rosmarinic acid (&lt;strong&gt;4&lt;/strong&gt;), and citrusin C (&lt;strong&gt;5&lt;/strong&gt;). Compound &lt;strong&gt;1&lt;/strong&gt; showed an antinociceptive effect at 316 mg/kg. A UHPLC method was validated for the quantification of &lt;strong&gt;1&lt;/strong&gt; in three different batches. The volatiloma analysis revealed that menthofuran (&lt;strong&gt;10&lt;/strong&gt;), β-caryophyllene (&lt;strong&gt;22&lt;/strong&gt;), spathulenol (&lt;strong&gt;31&lt;/strong&gt;), and caryophyllene oxide (&lt;strong&gt;32&lt;/strong&gt;) are the major constituents in the aerial parts. (±)−cunildone (&lt;strong&gt;27&lt;/strong&gt;) a new menthofuran derivative was isolated from the essential oil of the fresh aerial parts.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;div&gt;The results of these studies demonstrate the preclinical safety and validate the traditional use of &lt;em&gt;C. lythrifolia&lt;/em&gt; as an antinociceptive agent. Contribute to the chemical identification of the species and to the quality control and establish a method for quantitative analysis of the plant. Overall promoting the rational use and quality control of &lt;em&gt;C. lythrifolia&lt;/em&gt;.&lt;/div&gt;&lt;/div","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}