Journal of ethnopharmacology最新文献

{"title":"Systematic Multi-Model Toxicological Evaluation and Phytochemical Signatures of Carthamus tinctorius L., Scabiosa comosa, and Their Mixture: Acute and 28-Day Oral Toxicity Studies.","authors":"Wei Lv, Chenlu Zhang, Ming Xue, Yuehong Ma","doi":"10.1016/j.jep.2026.122515","DOIUrl":"https://doi.org/10.1016/j.jep.2026.122515","url":null,"abstract":"<p><strong>Ethnopharmacological relevance: </strong>Carthamus tinctorius L. and Scabiosa comosa Fisch. ex Roem. & Schult. are traditional medicinal plants used within the Traditional Chinese Medicine (TCM) and Traditional Mongolian Medicine (TMM) systems in China. Historically, the dried florets of C. tinctorius are administered as an aqueous decoction, typically at 3-10 g/day, and are prescribed to invigorate blood circulation and resolve blood stasis, whereas the capitula of S. comosa are prepared as a decoction or powder, typically at 6-9 g/day, and are utilized to clear heat and detoxify, with traditional indications for liver and pulmonary febrile diseases. However, comprehensive evaluations of their antioxidant properties and toxicological characteristics, particularly regarding their individual and combined applications, remain limited.</p><p><strong>Aim of the study: </strong>This study aimed to systematically characterize the phytochemical composition, antioxidant capacity, and safety profiles of C. tinctorius, S. comosa, and their combination using multiple extraction strategies and complementary biological models.</p><p><strong>Materials and methods: </strong>Water extracts, ethanol extracts, and sequential water-ethanol extracts were prepared and analyzed by LC-MS/MS and total flavonoid content determination. Their antioxidant capacities were evaluated using complementary in vitro assays. The toxicological profiles were investigated using BRL-3A rat hepatocytes, zebrafish embryo toxicity assays, acute oral toxicity studies in mice, and a 28-day repeated-dose oral toxicity study in Wistar rats.</p><p><strong>Results: </strong>LC-MS/MS profiling revealed diverse chemical constituents, including phenolic acids, flavonoids, coumarins, and iridoids. Notably, C. tinctorius exhibited the highest total flavonoid content (TFC), and sequential extraction significantly increased TFC compared to single-solvent extractions. In the antioxidant evaluations, S. comosa demonstrated the most potent antioxidant capacity with the lowest EC₅₀ values. Toxicity varied by concentration and extract type, with water extracts displaying the lowest toxicity. While high concentrations induced developmental abnormalities (e.g., mortality, oedema) in zebrafish, rodent models demonstrated high safety. Acute (5000 mg/kg) and 28-day repeated administrations caused no mortality or severe histopathological lesions, yielding only minor body weight and biochemical fluctuations.</p><p><strong>Conclusions: </strong>The present study provides a systematic chemical and toxicological evaluation of C. tinctorius, S. comosa, and their combination. These extracts exhibited antioxidant potential and favorable safety profiles in multiple experimental models, providing important evidence for their further development as medicinal or functional food-related products.</p>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":" ","pages":"122515"},"PeriodicalIF":6.8,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149207199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spermacoce stachydea DC. (Rubiaceae), a Traditionally Used Antimalarial Plant: Phytochemical Profiling, Antioxidant and Antiplasmodial Activities, Host-Protective Effects, and Preclinical Safety Assessment.","authors":"Saamou Isaac Boni, Kouliga Benjamin Koama, Domonbabele François de Sales Hien, Mariam Youba, Manon Vitou, Virginie Vaissayre, Zachari Kabre, Stephane Dussert, Sylvie Morel, Françoise Fons, Rakiswende Serge Yerbanga, Thierry Lefevre, Mohamed Haddad, Abdoulaye Diabate, Nâg-Tero Roland Meda","doi":"10.1016/j.jep.2026.122518","DOIUrl":"https://doi.org/10.1016/j.jep.2026.122518","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Ethnopharmacological relevance: &lt;/strong&gt;Spermacoce stachydea (Rubiaceae) is traditionally used for malaria treatment across West Africa, yet its phytochemical composition, antioxidant properties, antiplasmodial activity, and safety profile remain insufficiently characterised. This study aimed to provide a comprehensive preclinical characterisation for its ethnomedicinal use in malaria management.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;The whole-plant methanolic extract was profiled by ultra-high-performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS) and gas chromatography-mass spectrometry (GC-MS). Total phenolic (TPC), flavonoid (TFC), and condensed tannin (CTC) contents were quantified, and antioxidant capacity was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC) assays. Antiplasmodial activity was evaluated in vitro against Plasmodium falciparum 3D7 using a growth inhibition assay and in vivo in Plasmodium berghei ANKA-mice using a four-day suppressive test at 125, 250, and 500 mg/kg b.wt., and curative and prophylactic models at 250 mg/kg b.wt. Host-protective effects defined as the capacity of the extract to attenuate malaria-induced physiological disturbances including anaemia, organ enlargement, and hepatic and renal dysfunction were evaluated at 250 mg/kg b.wt. through restorative and protective assays encompassing physiological, haematological, biochemical analyses. Safety was assessed following single oral administration at 2000 and 5000 mg/kg b.wt. and repeated oral administration at 20, 200, and 2000 mg/kg b.wt./day in accordance with Organisation for Economic Co-operation and Development (OECD) guidelines 423 and 407, respectively.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Phytochemical profiling revealed a diverse array of bioactive metabolites including flavonoids, terpenoids, coumarins, iridoids, alkaloids, and polyphenols. The extract exhibited a flavonoid-dominant phenolic profile (TPC: 33.99 ± 0.07 mg GAE/100 mg; TFC: 7.57 ± 0.03 mg QE/100 mg) and strong DPPH radical-scavenging activity reached 774 ± 3.9 μmol AAE/g, compared with 647 ± 3.5 and 766 ± 2.9 μmol AAE/g for Trolox and quercetin, respectively. In vitro antiplasmodial activity was significant (IC&lt;sub&gt;50&lt;/sub&gt; = 6.30 ± 0.13 μg/mL) with negligible haemolysis (&lt;1%). In vivo, parasitaemia suppression reached 88.10 ± 6 % at 500 mg/kg (suppressive model), with good curative (58.40 ± 14.70%) and moderate prophylactic (35.70 ± 5.60%) activities at 250 mg/kg b.wt.. The extract attenuated malaria-associated physiological disturbances including anaemia, organ enlargement, and hepatic and renal dysfunction while demonstrated hepatoprotective effects. No mortality or consistent treatment-related toxicological alterations were observed after a single oral dose up to 5000 mg/kg b.wt. or repeated adminis","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":" ","pages":"122518"},"PeriodicalIF":6.8,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149194638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Predicting the potential mechanism of radix chimonanthi pracecocis in treating osteoarthritis by network pharmacology analysis combined with experimental validation\" [J. Ethnopharmacol. 331, (2024), 118231].","authors":"Xudong Zhang, Dongwen Wu, Lukai Zhang, Hongyan Zhang, Liping Yang, Li Wei, Huimin Mei, Liying Luo, Zong Jiang, Cong Huang","doi":"10.1016/j.jep.2026.122503","DOIUrl":"https://doi.org/10.1016/j.jep.2026.122503","url":null,"abstract":"","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":" ","pages":"122503"},"PeriodicalIF":6.8,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149194651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of the mechanism of Huangqi Guizhi Wuwu Decoction in the treatment of Diabetic Peripheral Neuropathy with Qi Deficiency and Blood Stasis based on the dynamic metabolic trajectory analysis.","authors":"Jingyu Wen, Li Yan, Yujie Xiang, Xu Wang, Cheng Fang, Shuyi Ru, Xinyue Zhang, Ziyi Liu, Xiuhong Wu","doi":"10.1016/j.jep.2026.122422","DOIUrl":"https://doi.org/10.1016/j.jep.2026.122422","url":null,"abstract":"<p><strong>Ethnopharmacological relevance: </strong>Huangqi Guizhi Wuwu Decoction (HGWD), a classic Traditional Chinese Medicine formula, has shown clinical efficacy in the treatment of diabetic peripheral neuropathy (DPN) with Qi deficiency and blood stasis syndrome. However, its pharmacodynamic material basis and underlying therapeutic mechanisms remain unclear.</p><p><strong>Purpose: </strong>This study aimed to systematically elucidate the pharmacodynamic material basis and therapeutic mechanisms of HGWD in the treatment of DPN using an integrated Chinmedomics strategy.</p><p><strong>Materials and methods: </strong>A rat model of DPN with Qi deficiency and blood stasis syndrome was established by streptozotocin (STZ) injection combined with dietary intervention and exhaustive swimming. HGWD was administered continuously for 8 weeks to assess its therapeutic effects. In addition to conventional pharmacological assessments, ANOVA-simultaneous component analysis (ASCA) was applied to urinary metabolomics analysis to dynamically track metabolic trajectories and characterize time-dependent changes in disease-related urinary biomarkers. Blood-absorbed components in the HGWD-treated group were characterized using UHPLC-QE-Orbitrap-MS. Correlation analysis was then performed among urinary biomarkers, key pharmacodynamic indicators, and in vivo active components. Molecular docking, molecular dynamics simulations, and in vitro experiments were further conducted to validate the core active components and their corresponding targets.</p><p><strong>Results: </strong>HGWD significantly improved neurological function and alleviated pathological damage in the DPN rat model. Urinary metabolomics analysis identified 28 disease-related urinary biomarkers that were markedly modulated by HGWD treatment. Under conditions of confirmed therapeutic efficacy, 98 blood-absorbed components, including 63 prototype compounds and 35 metabolites, were identified. Integrated correlation analysis and network pharmacology revealed eight key pharmacodynamic components and three core targets closely associated with the therapeutic effects of HGWD. Among these components, astragaloside II, paeoniflorin, and quercetin were identified as major bioactive compounds, which may exert therapeutic effects by regulating the protein expression of P-gp, PTP1B, and MMP2.</p><p><strong>Conclusion: </strong>This study systematically clarified the pharmacodynamic material basis and therapeutic mechanisms of HGWD in the treatment of DPN using an integrated Chinmedomics approach. The integrated analysis identified 17 urinary biomarkers, 8 core components, and 3 core targets, namely ABCB1, MMP2, and PTPN1, which were further supported by molecular simulations and in vitro validation These findings provide a comprehensive strategy for deciphering the mechanisms of Traditional Chinese Medicine formulas and support the potential application of HGWD in DPN treatment.</p>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":" ","pages":"122422"},"PeriodicalIF":6.8,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149182357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects and possible mechanisms of Salvia miltiorrhiza-derived interventions in animal models of osteoarthritis: a preclinical systematic review and meta-analysis.","authors":"Dongping Wan, Danyuan Wang, Yu Cui, Tao Chen, Chengcheng Zhang, Yuhang Liu, Ming Li, Zhong Li, Taotao Ren, Bing Du","doi":"10.1016/j.jep.2026.122511","DOIUrl":"https://doi.org/10.1016/j.jep.2026.122511","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Ethnopharmacological relevance: &lt;/strong&gt;Salvia miltiorrhiza Bunge, commonly known as Danshen, is a traditional Chinese medicinal herb historically used for blood stasis- and pain-related disorders. Its extracts and bioactive constituents have shown anti-inflammatory, antioxidant, anti-apoptotic, and tissue-protective effects, suggesting potential value for osteoarthritis (OA).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Aim of the study: &lt;/strong&gt;This systematic review and meta-analysis evaluated the protective effects and possible mechanisms of Salvia miltiorrhiza-derived interventions in animal models of OA.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Materials and methods: &lt;/strong&gt;PubMed, EMBASE, Web of Science, Scopus, CNKI, and Wanfang were searched from inception to March 2026. Controlled in vivo animal studies assessing Salvia miltiorrhiza-derived interventions or related constituents in OA models were included. Primary outcomes were histological cartilage damage scores, including OARSI and Mankin scores. Secondary outcomes included inflammatory cytokines, cartilage matrix metabolism markers, apoptosis-related indicators, and oxidative stress markers. Standardized mean differences (SMDs) with 95% confidence intervals (CIs) were calculated.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Twenty-two studies involving 456 animals were included. Salvia miltiorrhiza-derived interventions significantly attenuated histological cartilage damage, as reflected by lower OARSI scores (SMD = -4.20, 95% CI -5.31 to -3.09; I&lt;sup&gt;2&lt;/sup&gt; = 69.26%) and Mankin scores (SMD = -6.50, 95% CI -8.50 to -4.50; I&lt;sup&gt;2&lt;/sup&gt; = 85.61%). Model type showed a significant subgroup difference for OARSI score, and intervention category showed a significant subgroup difference for Mankin score, suggesting possible sources of heterogeneity. Significant reductions were observed in serum IL-1β, serum IL-6, cartilage/chondrocyte IL-6, and serum TNF-α, whereas the reduction in cartilage TNF-α did not reach statistical significance. Salvia miltiorrhiza-derived interventions also increased Aggrecan and Collagen II expression and modulated MMP-13 expression, supporting an improvement in cartilage matrix homeostasis. Apoptosis-related analyses showed increased Bcl-2 expression and reduced caspase-3 expression and TUNEL-positive apoptotic cells, whereas the reduction in Bax expression was not statistically significant. Cartilage MDA levels were also significantly reduced. Leave-one-out sensitivity analysis indicated that the pooled OARSI result was robust. Although funnel plot asymmetry was observed, trim-and-fill analysis did not alter the direction of the pooled effect.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;Current preclinical evidence suggests that Salvia miltiorrhiza-derived interventions may attenuate cartilage structural damage in animal models of OA, with possible involvement of inflammatory responses, extracellular matrix metabolism, chondrocyte apoptosis, and oxidative stress. However, these findings should be interpreted cautiously beca","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":" ","pages":"122511"},"PeriodicalIF":6.8,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149182347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agriophyllum squarrosum oligosaccharides ameliorate inflammation and lipid accumulation in T2DM-induced MASLD via miR-155-regulated PPARγ signaling pathways.","authors":"Shuyin Bao, Meijun Lin, Xuan Wang, Qiong Wu, Junguo Yin, Ruibo Wu, Yiping Che, Qianqian Ma, Xiuzhi Wang, Wuliji Ao","doi":"10.1016/j.jep.2026.122462","DOIUrl":"https://doi.org/10.1016/j.jep.2026.122462","url":null,"abstract":"<p><strong>Ethnopharmacological relevance: </strong>Agriophyllum squarrosum (L.) Moq., is a traditional medicinal herb first recorded in the \"Four Medical Tantras\" during the 8th century AD. It is commonly employed in Mongolian medicine for various therapeutic diseases, including the dispelling epidemics, heat clearance, detoxification, and diuresis. This herb is known to inhibit \"He Yi,\" regulate \"Ba Da Gan,\" and is utilized in the treatment of \"Xi He Ri Xi Jing Bing\" which corresponds to modern diabetes and xiaoke in traditional Chinese medicine. Agriophyllum squarrosum oligosaccharides (AOS) are recognized as major bioactive constituents, however, the molecular mechanisms underlying their therapeutic effects on type 2 diabetes mellitus (T2DM)-related metabolic dysfunction-associated steatotic liver disease (MASLD) remain unclear.</p><p><strong>Aim of the study: </strong>This study investigates the therapeutic effects of AOS in T2DM-MASLD and explores the underlying mechanisms involving miR-155-mediated signaling pathways.</p><p><strong>Materials and methods: </strong>In vivo, db/db mice were treated with AOS, and hepatic lipid accumulation and insulin resistance were evaluated using biochemical and histological analyses. Hepatic expression of miR-155 and associated signaling proteins was observed by qRT-PCR, Western blotting, and immunohistochemistry. Exosomes derived from ATMs of db/db mice were co-cultured with AML-12 hepatocytes to investigate the role of exosomal miR-155 in MASLD progression. In vitro, inflammatory cytokine production and miR-155 expression were quantified using ELISA and qRT-PCR, while exosomal miR-155 secretion was evaluated in glucolipid-induced cellular injury models.</p><p><strong>Results: </strong>AOS treatment reduced hepatic steatosis and improved insulin resistance in db/db mice. AOS was associated with decreased hepatic miR-155 expression. Exosomal miR-155 derived from ATMs aggravated hepatocyte injury in the co-culture system, accompanied by increased expression of SREBP1c, NLRP3, and NF-κB p65. In vitro, AOS inhibited exosomal miR-155 secretion and downregulated the expression of SREBP1c, P2X7R, NF-κB p65, and inhibitor of IκBα, thus attenuating glucolipid metabolism-associated inflammatory responses and lipid dysregulation.</p><p><strong>Conclusion: </strong>AOS ameliorates inflammation, hepatic lipid accumulation, and insulin resistance in T2DM-associated MASLD, possibly via suppression of miR-155 and modulation of downstream inflammatory and lipogenic signaling pathways. These results identify AOS as a promising therapeutic candidate for treating T2DM-associated MASLD.</p>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":" ","pages":"122462"},"PeriodicalIF":6.8,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149182362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shisanwei Qinglan Powder attenuates ethanol-induced gastric mucosal injury in association with reduced NF-κB signalling and caspase activation.","authors":"Kanshen Ye, Haoqin Li, Zhizheng Qin, Zichen Zhu, Rui Wang, Jie Dou, Qingjia Ren, Xiaoya Liu, Fei Wang","doi":"10.1016/j.jep.2026.122505","DOIUrl":"https://doi.org/10.1016/j.jep.2026.122505","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Ethnopharmacological relevance: &lt;/strong&gt;Shisanwei Qinglan Powder (SQP) is a marketed Tibetan medicinal formula traditionally used for gastrointestinal disorders associated with \"peigen mubu\", including epigastric distension and pain, gastritis, and ulcer-related symptoms. However, experimental evidence for the complete formula, its systemically exposed constituents, and the mechanisms underlying gastric mucosal protection remains limited.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Aim of the study: &lt;/strong&gt;This study aimed to characterise the serum-associated constituents of SQP and evaluate its protective effects and molecular responses in acute ethanol-induced gastric mucosal injury.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Materials and methods: &lt;/strong&gt;Chemical profiling and serum pharmacochemistry were performed by liquid chromatography-tandem mass spectrometry. Network pharmacology and gastric-tissue transcriptomics were integrated to prioritise treatment-associated pathways. Pharmacological effects were evaluated in mice receiving SQP prophylactically before anhydrous ethanol challenge and in GES-1 cells receiving SQP after ethanol-induced injury, using viability, migration, apoptosis, macroscopic lesion, histological, oxidative-stress, and inflammatory measurements. The IKKβ/IκBα/NF-κB axis and caspase-8/caspase-3 activation were assessed by immunohistochemistry and western blotting. Selected compound-target pairs were further evaluated by molecular docking and 100-ns molecular dynamics simulations.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Thirty-two distinct serum-associated constituents were tentatively identified, and 185 targets were shared between SQP- and gastric ulcer-related datasets. Under the post-injury cell-intervention paradigm, SQP improved viability and migration, corrected oxidative and cytokine imbalances, and reduced total apoptosis from approximately 21.9% to 7.9% at 20 μg/mL. Under the prophylactic animal paradigm, SQP alleviated haemorrhage, erosion, and glandular disruption, and 0.52 g/kg significantly reduced both the ulcer index and damaged area. RNA sequencing identified 1,533 differentially expressed genes between the high-dose SQP and model groups, with enrichment in TNF, NF-κB and apoptosis-related pathways. In both models, SQP reduced phosphorylation of IKKβ, IκBα and NF-κB p65, suppressed caspase-8 and caspase-3 cleavage, and decreased gastric TUNEL positivity. Molecular docking and molecular dynamics simulations were used for computational prioritization of the TNF-bicuculline and CASP3-kaempferol pairs; these analyses indicated structural feasibility under the simulated conditions but did not establish direct binding or target engagement.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;SQP showed prophylactic gastroprote ctive effects in mice and post-injury protective and reparative effects in ethanol-injured GES-1 cells. Across these distinct experimental paradigms, the observed effects were associated with attenuation of oxidative stress, IKK/NF-κB-related inflammati","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":" ","pages":"122505"},"PeriodicalIF":6.8,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149182374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modified Danzhi Xiaoyao San ameliorates depression-associated dry eye by modulating sphingolipid metabolism across the liver, hippocampus, and lacrimal gland.","authors":"Xu Yan, Xie MinSi, Deng Qibo, Ji Jiajia, Zhou YuXi, Huang Yu, Xia Shuaishuai, Peng Qinghua, Yuan Zhiying","doi":"10.1016/j.jep.2026.122472","DOIUrl":"https://doi.org/10.1016/j.jep.2026.122472","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Ethnopharmacological relevance: &lt;/strong&gt;Modified Danzhi Xiaoyao San (MDZXYS) originates from Xue Ji's \"Internal Medicine Abstract\" (Ming Dynasty), which derived from the Song Dynasty Taiping Huimin Heji Jufang Xiao-Yao Powder by adding Moutan Cortex and Gardeniae Fructus. It is traditionally used to soothe the liver, relieve depression, and clear stagnant heat associated with liver-qi stagnation transforming into fire. Grounded in the theory that \"the liver opens into the eyes,\" liver-soothing and heat-clearing strategy has long been applied to treat ocular diseases. Previous studies have reported beneficial effects of Danzhi Xiaoyao San and related modified formulas on depression and dry eye; however, the metabolic basis of their effects across multiple tissues remains incompletely understood.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Aim of the study: &lt;/strong&gt;This study evaluated the therapeutic effects of MDZXYS in a rat model of depression-associated dry eye and characterized associated changes in sphingolipid metabolism across multiple tissues.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Materials and methods: &lt;/strong&gt;A rat model of depression-associated dry eye was established by combining chronic unpredictable mild stress (CUMS) with benzalkonium chloride (BAC) eye drops. low- and high-dose MDZXYS were orally administered, with venlafaxine as the positive control. Evaluations included assessments of depression-like behaviors, tear secretion, tear film break-up time (TBUT), and corneal fluorescein staining. Histopathology, ELISA for inflammatory cytokines, targeted lipidomics, and Western blotting for Sptlc2 and nSMase were performed on hippocampal, hepatic, lacrimal gland, and serum samples.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;MDZXYS attenuated hippocampal neuronal abnormalities in the CA1, CA3, and DG regions and increased BDNF expression. It also improved pathological changes in the liver and lacrimal glands and preserved conjunctival goblet cells. MDZXYS increased tear secretion, prolonged TBUT, and reduced corneal fluorescein staining. Behavioral assessments showed increased sucrose preference and locomotor activity, together with reduced immobility time. MDZXYS also reduced the pro-inflammatory cytokines IL-1β, IL-6, and TNF-α and increased IL-4 levels in the liver, serum, and lacrimal glands. Lipidomic profiling and KEGG enrichment analysis showed that differentially altered metabolites across the hippocampus, liver, lacrimal gland, and serum were significantly enriched in the sphingolipid metabolism pathway. Among the altered sphingolipid metabolites, Cer(d18:1/20:1), Cer(d18:1/22:1), Cer(d18:1/24:1), HexCer(18:1/24:1), SM(d18:1/16:0), and SM(d18:1/20:1) showed significant associations with depressive-like behaviors and dry eye-related phenotypes. MDZXYS treatment partially reversed these disease-associated sphingolipid alterations. Western blotting further revealed tissue-specific changes in sphingolipid metabolism-related protein expression: MDZXYS increased nSMase and decrease","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":" ","pages":"122472"},"PeriodicalIF":6.8,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149167483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Tianma Gouteng granules decreases the susceptibility of Parkinson's disease by inhibiting ALOX15-mediated lipid peroxidation\" [J. Ethnopharmacol. 256, (2020), 112824].","authors":"Ying-Nan Jiang, Yong-Zhi Guo, Dan-Hua Lu, Ming-Hai Pan, Hai-Zhi Liu, Gen-Long Jiao, Wei Bi, Hiroshi Kurihara, Yi-Fang Li, Wen-Jun Duan, Rong-Rong He, Xin-Sheng Yao","doi":"10.1016/j.jep.2026.122501","DOIUrl":"https://doi.org/10.1016/j.jep.2026.122501","url":null,"abstract":"","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":" ","pages":"122501"},"PeriodicalIF":6.8,"publicationDate":"2026-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149166244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Tianma Gouteng granules decreases the susceptibility of Parkinson's disease by inhibiting ALOX15-mediated lipid peroxidation\" [J. Ethnopharmacol. 256, (2020), 112824].","authors":"Ying-Nan Jiang, Yong-Zhi Guo, Dan-Hua Lu, Ming-Hai Pan, Hai-Zhi Liu, Gen-Long Jiao, Wei Bi, Hiroshi Kurihara, Yi-Fang Li, Wen-Jun Duan, Rong-Rong He, Xin-Sheng Yao","doi":"10.1016/j.jep.2026.122456","DOIUrl":"https://doi.org/10.1016/j.jep.2026.122456","url":null,"abstract":"","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":" ","pages":"122456"},"PeriodicalIF":6.8,"publicationDate":"2026-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149166950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}