Dan Nana Osei Bonsu PhD, Natasha Mitchell BSc, Claire Jeanes BSc (Hons), Julianne Henry PhD
{"title":"Investigation of a Y-chromosome screening approach for sexual assault evidence using the QIAGEN Investigator Casework GO! kit","authors":"Dan Nana Osei Bonsu PhD, Natasha Mitchell BSc, Claire Jeanes BSc (Hons), Julianne Henry PhD","doi":"10.1111/1556-4029.70027","DOIUrl":"10.1111/1556-4029.70027","url":null,"abstract":"<p>Following an alleged sexual assault, a victim may undergo a medical examination whereby internal or external bodily swabs may be collected and submitted to a forensic laboratory to be screened for the presence of semen. At Forensic Science SA (FSSA), this involves microscopic examination of a smear prepared from each swab for the presence of sperm. Microscopic detection of sperm is confirmatory for semen, but the process is very time-consuming, especially where very low levels are present. Moreover, microscopy results are not a reliable predictor of the amount of male DNA that might be recovered. SWGDAM recommends a direct-to-DNA approach as an efficient alternative. Therefore, we investigated the Qiagen Casework GO! kit (CWG), in conjunction with the Y-chromosome marker in the Quantifiler Trio DNA quantification kit, for the rapid screening of sexual assault swabs for the presence of male DNA (termed ‘Y-screen’). We initially investigated two swab types, the Copan cotton swab (currently used in Forensic Examination Collection Kits (FECK) submitted to FSSA) and the Sarstedt Forensic XL swab (a Forensic DNA grade alternative). The optimal size of the swab cutting and the volume of CWG lysis mix for the Y-screen was determined to be 1/8 and 100 μL, respectively, which ensured cost minimization and sample preservation. The performance of the optimized Y-screen method was compared with conventional microscopy using a series of simulated sexual assault swabs constructed by spiking female buccal swabs with serial dilutions of semen. For Copan cotton swabs, all samples with detectable sperm by microscopy had detectable levels of male DNA using the Y-screen. The Y-screen also showed greater sensitivity than microscopy for this swab type, detecting male DNA in 8% of samples where sperm was not observed. In contrast, 36% of Forensic XL swabs gave undetectable male DNA when sperm was observed by microscopy, which indicated that the Y-screen approach with CWG may not be compatible with all swab types. A casework trial of the Y-screen approach using 46 FECK swabs (Copan cotton swabs) submitted across 29 cases confirmed the higher sensitivity of the Y-screen approach as compared to conventional microscopy. Here, 44% of FECK swabs that had no observable sperm by microscopy had detectable male DNA using the Y-screen. There were no instances where the FECK swabs with observable sperm by microscopy had undetectable male DNA using the Y-screen. Our study confirmed the utility of the Y-screen approach using CWG to expedite the downstream DNA analysis of FECK swab samples in operational casework.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"70 3","pages":"1133-1148"},"PeriodicalIF":1.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1556-4029.70027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143672125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kenneth Aschheim DDS, János Vág DMD, PhD, Botond Simon DMD, PhD
{"title":"A mathematical model for scientifically defining the class characteristics of the human anterior maxilla—Part 1: The dental arch","authors":"Kenneth Aschheim DDS, János Vág DMD, PhD, Botond Simon DMD, PhD","doi":"10.1111/1556-4029.70006","DOIUrl":"10.1111/1556-4029.70006","url":null,"abstract":"<p>Bitemark recognition reliability in forensics has been criticized for lacking objectivity and empirical support. Despite doubts about classifying these injuries as bitemarks, pattern injuries must still be legally documented and analyzed. Forensic odontology can benefit from aesthetic dentistry by adopting metric analysis methods to define human dentition and objectively assess whether a pattern resembles a dental arch. In addition, past research on the challenges of individualizing bitemarks has inadvertently also defined these class characteristics. Although objectively quantifying these metrics is critical, prospective research must prove their uniqueness to the human species as well as their dependable transference to substrates such as skin. To explore and qualitatively define the mathematical characteristics of variations in the maxillary dental arch, an under-researched aspect of forensic odontology, two scanners collected digital maxilla scans from 100 participants (50% male, 50% female). The arch shape's intercuspal distance and parabolic curvature (<i>y</i> = <i>ax</i><sup>2</sup> + <i>bx</i> + <i>c</i>) were defined, recorded, and analyzed. The mean intercuspal distance of maxillary canines was 33.8 mm (SD: 2.25 mm, 29.0–39.6 mm). The mean fitted curve created by Linear Mixed Model (LMM) for Quadratic Regression Analysis was a mean of <i>y</i>-mean<sub>Total</sub> = 0.040<i>x</i><sup>2</sup> − 0.0008<i>x</i> − 1.581, an upper limit of <i>y</i>-higher<sub>Total</sub> = 0.047<i>x</i><sup>2</sup> − 0.00039<i>x</i> + 2.593, and a lower limit of <i>y</i>-lower<sub>Total</sub> = 0.032<i>x</i><sup>2</sup> − 0.0012<i>x</i> − 5.754. In addition, sexual dimorphism using quadratic analysis was not established. Quadratic regression analysis establishes an objective framework for characterizing the maxillary dental arch. Results indicate that the arches of human dentitions share class characteristics within a narrow range, and can also offer a framework for the assessment of dentitions across species.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"70 3","pages":"1074-1088"},"PeriodicalIF":1.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1556-4029.70006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Victor G. Petreca PhD, DNP, Kalliopi Tzani-Pepelasis PhD, Maria Ioannou PhD, Alice Siggery PhD, Lauren McBride BA, Elora Barros, Ann W. Burgess DNSc
{"title":"The grip of crime: Analyzing strangulation and asphyxiation typologies in homicide cases","authors":"Victor G. Petreca PhD, DNP, Kalliopi Tzani-Pepelasis PhD, Maria Ioannou PhD, Alice Siggery PhD, Lauren McBride BA, Elora Barros, Ann W. Burgess DNSc","doi":"10.1111/1556-4029.70021","DOIUrl":"10.1111/1556-4029.70021","url":null,"abstract":"<p>Strangulation-related homicides represent a complex and under-researched form of violent crime, characterized by distinct behavioral and situational elements. The aim of the research was to identify perpetrator typologies in strangulation homicides and to analyze their relationships with victim characteristics and crime scene factors. A retrospective analysis of 200 systematically sampled cases from the Radford/Florida Gulf Coast University Serial Killer Database employed Smallest Space Analysis (SSA) to examine 42 behavioral variables. The analysis revealed four primary typologies: Excessive (characterized by severe violence beyond lethality, including mutilation and torture), intended (marked by premeditation and organizational elements), personal (distinguished by intimate engagement and direct manual methods), and sexual (defined by sexual assault and sadistic behaviors). Statistical analysis demonstrated significant correlations between offender–victim relationships, crime scene characteristics, and degrees of violence within each typological category, with 97% of cases fitting distinctly into one category and 3% showing hybrid characteristics. The findings provide empirically based frameworks for forensic profiling and inform specific investigative strategies aligned with each typological pattern. These results equip law enforcement with targeted investigation and risk assessment approaches, potentially leading to more accurate offender identification and prevention efforts. The research advances the understanding of psychological and situational factors in strangulation-related homicides, contributing to both forensic literature and practical applications in criminal investigations.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"70 3","pages":"1094-1104"},"PeriodicalIF":1.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1556-4029.70021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143672127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alexandra R. Klales PhD, Kate M. Lesciotto JD, PhD
{"title":"Reevaluating skeletal sex estimation practices in forensic anthropology","authors":"Alexandra R. Klales PhD, Kate M. Lesciotto JD, PhD","doi":"10.1111/1556-4029.70014","DOIUrl":"10.1111/1556-4029.70014","url":null,"abstract":"<p>Understanding practitioner preferences in method selection and reporting for skeletal sex estimation is a necessary step toward the standardization of biological profile estimation within forensic anthropological practice in the United States. To better understand the current state of skeletal sex estimation, an electronic survey was sent via omnichannel distribution methods, targeted to individuals practicing skeletal sex estimation in forensic anthropology. One hundred eighteen individuals responded, answering questions about their educational and training background, case experience, practices, and preferences for skeletal sex estimation, and preferences for future method development. Most respondents use both qualitative and quantitative approaches to estimate skeletal sex (99.0%) and employ multiple methods for casework. The pelvis was preferred for morphological approaches, and the Fordisc program [2005, FORDISC 3: Personal computer forensic discriminate functions] was preferred for metric approaches to skeletal sex estimation. Respondents placed emphasis on the validity and reliability of specific methods, their experience and comfort level with applying specific methods, and utilizing methods that did not require expensive equipment. There was considerable variation in how the final sex estimate was determined and reported, with most either giving preference to the pelvis (36.1%) or reporting all methods but basing the final estimation on experience (39.2%). These results were largely similar to the results from a similar survey conducted in 2012, including a preference for using the pelvis for morphological sex estimation; however, the introduction and adoption of new sex estimation methods since 2012 have changed the landscape of practitioner preferences.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"70 3","pages":"825-834"},"PeriodicalIF":1.5,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Paige Riley-Carrier MS, Mindy Greco PhD, Aldo D'Ottavio MS, Kiersten Schiliro BS, Christopher A. Tipple PhD
{"title":"Screening for clandestine graves via the analysis of infrared images and temperature metrics","authors":"Paige Riley-Carrier MS, Mindy Greco PhD, Aldo D'Ottavio MS, Kiersten Schiliro BS, Christopher A. Tipple PhD","doi":"10.1111/1556-4029.70019","DOIUrl":"10.1111/1556-4029.70019","url":null,"abstract":"<p>Locating a clandestine grave is a challenging yet crucial step in a criminal investigation and eventual prosecution. Many techniques have been used to locate clandestine graves, including light detection and ranging and ground penetrating radar. Recently, thermal imaging has also been evaluated as a method for this task. However, questions such as the best time of day for use and the technique's longevity still exist. This study served as a means to address these topics. First, six simulated clandestine graves were created in a forested environment. Then, the graves were monitored for two years with long-wave infrared cameras. Temperature probes were also employed to collect surface and subsurface level data. After collection, a visual evaluation of the thermal images was conducted, and then an image processing code was employed to obtain image temperature data. From there, the data were evaluated for possible statistically significant temperature differences. Temperature probe data and camera data were also compared. In addition to the visual differences noted in the images, statistically significant differences were measured between the regions of interest and the controls throughout the experimental time period. Specifically, the location of the grave and time of day influenced the temperature response. For this specific study location, it was determined that image collection between 1700 and 0800 h provided the greatest ability to discern the regions of interest from the surrounding undisturbed areas. The temperature probes presented differences when inter- and intra-comparisons were performed. Differences also existed between the temperature probe and camera data.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"70 3","pages":"889-904"},"PeriodicalIF":1.5,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kate Moghadam PhD, Diane Beauchemin PhD, Claude Dalpé PhD
{"title":"Laser-induced breakdown spectroscopy/laser ablation coupled to inductively coupled plasma mass spectrometry (LIBS/LA-ICPMS) for the forensic screening and discrimination of lead-free solders","authors":"Kate Moghadam PhD, Diane Beauchemin PhD, Claude Dalpé PhD","doi":"10.1111/1556-4029.70022","DOIUrl":"10.1111/1556-4029.70022","url":null,"abstract":"<p>The tandem LIBS/LA-ICPMS technique is a desirable tool for the multi-elemental determination, characterization, and classification of alloys as forensic evidence. In this study, LIBS/LA-ICPMS is validated for the forensic evaluation of lead-free solder alloys, which form valuable evidence from post-blast crime scenes involving homemade and improvised explosive devices. LIBS/LA-ICPMS is competitive with other spectroscopic-based forensic techniques as it is in situ, analyzes samples directly, and requires minimal destruction of the exhibit. Following a one-standard calibration technique, nine major (alloying metals) and trace elements (impurities or additives) are quantified in lead-free solders. Optimizing laser parameters and using Pb as a naturally occurring internal standard are shown to compensate for mass-dependent drift and matrix effects. The quantitative results of Pb-free certified reference materials align with certificate values and with results from two techniques in a cross-validation comparison, including electrothermal vaporization-inductively coupled plasma optical emission spectrometry and neutron activation analysis. Utilizing peak ratios in a model of principal component analysis is presented to identify key compositional differences among solders and provide a visual model for solder discrimination. Outcomes of this approach demonstrate the potential for associating or discriminating lead-free solders, including different solders from the same manufacturer. Together, this technique can establish chemical concordance among known and questioned materials and offers a utilitarian approach for the forensic assessment of trace evidence.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"70 3","pages":"980-994"},"PeriodicalIF":1.5,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1556-4029.70022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Carl J. Stella BSc (Hons), Todd W. Bille MSc, Georgina E. Meakin PhD, Roland A. H. van Oorschot PhD
{"title":"Potential for DNA loss during collection and packaging of fired cartridge cases at a crime scene","authors":"Carl J. Stella BSc (Hons), Todd W. Bille MSc, Georgina E. Meakin PhD, Roland A. H. van Oorschot PhD","doi":"10.1111/1556-4029.70016","DOIUrl":"10.1111/1556-4029.70016","url":null,"abstract":"<p>DNA recovery from fired cartridge cases (FCCs) found at a crime scene is complicated by the limited DNA amounts typically available for collection. While this challenge is unavoidable, recovery rates may be improved by optimizing handling and packaging processes to better preserve the available DNA. Here we compared existing methods of collecting and packaging FCCs at a crime scene and assessed the utility of a novel cartridge collection device to determine which combination best preserves DNA integrity and amounts. FCCs loaded with touch DNA or blood and some DNA-free FCCs were collected and placed into paper or plastic packaging by one of 12 methods. Packages were handled in a manner that mimicked handling during casework, after which the location of DNA within the packaging was assessed to determine where transfer/loss had occurred. We observed that DNA can be lost from FCCs to an examiner's glove during collection, to the inside of packaging after handling and transport, and between FCCs in the same packaging. The novel cartridge collection device mitigated this transfer in many cases and could be considered a means of preserving DNA on FCCs. The results demonstrate the potential for preserving DNA by avoiding direct contact between the FCC and other items.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"70 3","pages":"944-953"},"PeriodicalIF":1.5,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Damaris Roberta Alves Menezes De Sousa DDS, Carolina de Paula Rossetto Lisboa DDS, Ademir Franco PhD, José Luiz Cintra Junqueira PhD, Anne Caroline Oenning PhD, Monikelly do Carmo Nascimento Narchini PhD, Mariana Quirino Silveira Soares PhD
{"title":"Human identification through smile photographs: Comparison of two methods based on selfies","authors":"Damaris Roberta Alves Menezes De Sousa DDS, Carolina de Paula Rossetto Lisboa DDS, Ademir Franco PhD, José Luiz Cintra Junqueira PhD, Anne Caroline Oenning PhD, Monikelly do Carmo Nascimento Narchini PhD, Mariana Quirino Silveira Soares PhD","doi":"10.1111/1556-4029.70020","DOIUrl":"10.1111/1556-4029.70020","url":null,"abstract":"<p>This study aimed to compare the accuracy of two human identification methods based on selfies. The maxillary teeth of five adults were three-dimensionally acquired via intraoral scanning—being the simulated <i>postmortem</i> (PM) records. To simulate <i>antemortem</i> (AM) records, the five individuals took selfies of their smile. For each participant, five additional volunteers with similar ages and population affinities were requested to take selfies, which were used as foil AM records. Then, five sets containing the intraoral scan image, the respective selfie, and five foil selfies were assembled and sent to 29 forensic odontologists. The experts applied two analytical methods: the smile line and dental superimposition. The experts initially excluded the foil selfies and then identified the correct PM and AM match. Both methods allowed the same number of correct (<i>n</i> = 123) and incorrect (<i>n</i> = 22) exclusions of foils (<i>p</i> > 0.05). The smile line and the dental superimposition methods resulted in 94 and 86 correct, as well as 51 and 59 incorrect matches (<i>p</i> > 0.05), respectively. These methods must be cautiously considered and combined with other methods for safer forensic practices.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"70 3","pages":"1181-1187"},"PeriodicalIF":1.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hasan Abder-Rahman MD, PhD, Imad Al-Abdallat MD, Lana N. Qaqish MD, Ameera Elqasass MD, Asma Al-Shaeb MD, Leen Abuzaid MD, Younes F. Samara MD
{"title":"Understanding the link between autism and self-harm from a forensic lens","authors":"Hasan Abder-Rahman MD, PhD, Imad Al-Abdallat MD, Lana N. Qaqish MD, Ameera Elqasass MD, Asma Al-Shaeb MD, Leen Abuzaid MD, Younes F. Samara MD","doi":"10.1111/1556-4029.70026","DOIUrl":"10.1111/1556-4029.70026","url":null,"abstract":"<p>Autism spectrum disorder (ASD) is a complex neurodevelopmental condition characterized by social communication difficulties, restricted interests, and repetitive behaviors. Individuals with ASD, particularly females, are at an increased risk of self-injurious behavior (SIB), which can sometimes be misinterpreted as signs of physical abuse. This case report describes a 17-year-old female with ASD and ADHD and limited verbal language skills who presented to the emergency room with multiple skin lesions resembling second-degree burns, raising concerns of child abuse. Initial assessments by the emergency and dermatology teams were inconclusive, and plastic surgeons suggested the lesions were burns. However, forensic specialists noted inconsistencies with burn patterns, including the absence of blisters and redness around newer lesions. A review of the patient's psychiatric records revealed a history of repetitive self-harm, specifically aggressive scratching. Additional history from the family confirmed that the injuries were self-inflicted. Distinguishing between self-harming injuries and physical abuse in patients with ASD can be challenging, especially since both can coexist. This necessitates thorough assessment and experienced forensic consultation to avoid misdiagnosis, which can lead to significant legal and emotional consequences. To conclude, accurate diagnosis of self-harm versus abuse in patients with autism requires detailed investigation, consideration of psychiatric history, and forensic expertise. Moreover, early and correct diagnosis is crucial to prevent misattributed allegations of abuse and facilitate appropriate medical and psychiatric care for individuals with ASD.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"70 3","pages":"1202-1207"},"PeriodicalIF":1.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhengzhe Zang BE, Peibin Wang BE, Haoyuan Li BE, Jing Jin PE, Yu Wang BE, Yiyue Cao BE
{"title":"Research on temperature-dependent visualization and enhancement mechanism of latent fingermarks deposited on steel container","authors":"Zhengzhe Zang BE, Peibin Wang BE, Haoyuan Li BE, Jing Jin PE, Yu Wang BE, Yiyue Cao BE","doi":"10.1111/1556-4029.70024","DOIUrl":"10.1111/1556-4029.70024","url":null,"abstract":"<p>In arson cases, the accelerants are typically stored in steel containers where fingermarks may be preserved. To explore the effects of heat on latent fingermark visualization deposited on steel surfaces and the corresponding mechanisms, 304 stainless steel and tinplate were selected. Coaxial illumination and cyanoacrylate fuming were employed to visualize fingermarks. An optical microscope (OM) equipped with a hot stage was utilized to characterize the fingermark ridges evolution in situ. Additionally, the scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDS) were used to analyze fingermark micromorphology and variations in fingermark composition. The results showed that below 500°C, the visibility of fingermarks on both steel surfaces decreased as the temperature increased, and no ridges were visible at 500°C. This was due to the decomposition/evaporation of organic compounds in the fingermarks caused by high temperatures. However, their outlines began to reappear at 600°C, and ridges became visible to the naked eye at 800°C. This was due to the fact that at temperatures exceeding 600°C, oxidative, electrochemical, and salt corrosion between fingermarks and substrates were accelerated, achieving fingermark enhancement. For tinplate, due to its antioxidant properties and the corrosion resistance provided by FeS<sub>2</sub>, it exhibited less apparent oxidation and salt corrosion. Comparatively, fingermark visualization on 304 stainless steel was superior to that on tinplate. This study demonstrated that fingermarks could be retained on steel surfaces even after exposure to high temperatures with different mechanisms related to the chemical composition of steel, which provided crucial data for fire investigations.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"70 3","pages":"1012-1025"},"PeriodicalIF":1.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}