Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes最新文献_第9页

Microbiome analyses of poultry feeds: Part I. Comparison of five different DNA extraction methods. 家禽饲料微生物组分析：第一部分：五种不同 DNA 提取方法的比较。

IF 2 4区农林科学

Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes Pub Date : 2024-01-01 Epub Date: 2024-05-23 DOI: 10.1080/03601234.2024.2353002

E G Olson, D K Dittoe, A C Micciche, D A Stock, P M Rubinelli, M J Rothrock, S C Ricke

{"title":"Microbiome analyses of poultry feeds: Part I. Comparison of five different DNA extraction methods.","authors":"E G Olson, D K Dittoe, A C Micciche, D A Stock, P M Rubinelli, M J Rothrock, S C Ricke","doi":"10.1080/03601234.2024.2353002","DOIUrl":"10.1080/03601234.2024.2353002","url":null,"abstract":"Given extensive variability in feed composition, the absence of a dedicated DNA extraction kit for poultry feed underscores the need for an optimized extraction technique for reliable downstream sequencing analyses. This study investigates the impact of five DNA extraction techniques: Qiagen QIAamp DNA Stool Mini Kit (Qiagen), modified Qiagen with Lysing Matrix B (MQ), modified Qiagen with celite purification (MQC), polyethylene glycol (PEG), and 1-Day Direct. Genomic DNA amplification and Illumina MiSeq sequencing were conducted. QIIME2-2021.4 facilitated data analysis, revealing significant diversity and compositional differences influenced by extraction methods. Qiagen exhibited lower evenness and richness compared to other methods. 1-Day Direct and PEG enhanced bacterial diversities by employing bead beating and lysozyme. Despite similar taxonomic resolution, the Qiagen kit provides a rapid, consistent method for assessing poultry feed microbiomes. Modified techniques (MQ and MQC) improve DNA purification, reducing bias in commercial poultry feed samples. PEG and 1-Day Direct methods were effective but may require standardization. Overall, this study underscores the importance of optimized extraction techniques in poultry feed analysis, with potential implications for future standardization of effective methods.","PeriodicalId":15720,"journal":{"name":"Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes","volume":" ","pages":"378-389"},"PeriodicalIF":2.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141081725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Root exudation of glyphosate in Eucalyptus urophylla S.T. Blake. 草甘膦在 Eucalyptus urophylla S.T. Blake 根部的渗出。

IF 1.4 4区农林科学

Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes Pub Date : 2024-01-01 Epub Date: 2024-11-06 DOI: 10.1080/03601234.2024.2422719

Rodrigo Eduardo Barros, Matheus Mendes Reis, Leonardo David Tuffi Santos, João Paulo Fernandes Tiago, Érika Manuela Gonçalves Lopes, Luan Mateus Silva Donato

{"title":"Root exudation of glyphosate in Eucalyptus urophylla S.T. Blake.","authors":"Rodrigo Eduardo Barros, Matheus Mendes Reis, Leonardo David Tuffi Santos, João Paulo Fernandes Tiago, Érika Manuela Gonçalves Lopes, Luan Mateus Silva Donato","doi":"10.1080/03601234.2024.2422719","DOIUrl":"10.1080/03601234.2024.2422719","url":null,"abstract":"Glyphosate stands out in the eucalyptus management, which makes it essential to know its behavior, its effects on the plant, and possible environmental impacts. This study aimed to identify and quantify the root exudation of glyphosate and aminomethylphosphonic acid (AMPA) by Eucalyptus urophylla with chromatographic and biological methods. The five glyphosate doses were tested (0, 360, 720, 1080 and 1440 g a.e ha-1) on E. urophylla plants. The physiological and intoxication evaluations were performed after herbicide application. Water samples remaining from the pots were used for chemical quantification of root exudation of glyphosate and AMPA in high-performance liquid chromatography. Cucurbita pepo plants were used as bioindicators of glyphosate in the water remaining in the pots after applying herbicide. The increase in glyphosate doses promoted linear growth in E. urophylla intoxication and significantly reduced total dry mass and root production. E. urophylla plants had their photosynthetic, transpiratory, and stomatal conductance rates reduced as the herbicide doses increased. The AMPA root exudation was not detected, but it was possible to identify the presence of glyphosate by bioassay and chemical methods. Root exudation of glyphosate by eucalyptus can result in lesser herbicide action in plant control and cause contamination of deeper soil layers.","PeriodicalId":15720,"journal":{"name":"Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes","volume":" ","pages":"749-757"},"PeriodicalIF":1.4,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pre-emergence herbicides mixture in soybeans: Amaranthus hybridus control and crop selectivity on cover crops soil. 大豆播前除草剂混合物：杂交苋在覆盖作物土壤上的控制和作物选择性。

IF 1.4 4区农林科学

Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes Pub Date : 2024-01-01 Epub Date: 2024-07-03 DOI: 10.1080/03601234.2024.2372920

Modesto Roque Mita, Kassio Ferreira Mendes

{"title":"Pre-emergence herbicides mixture in soybeans: Amaranthus hybridus control and crop selectivity on cover crops soil.","authors":"Modesto Roque Mita, Kassio Ferreira Mendes","doi":"10.1080/03601234.2024.2372920","DOIUrl":"10.1080/03601234.2024.2372920","url":null,"abstract":"The objective was to evaluate the efficacy of pre-emergence herbicides mixture applied to the soil with and without dead cover crops (Sorghum bicolor) for the control of Amaranthus hybridus L. (smooth pigweed) and its selectivity in soybeans. This study was structured in split plot (2 × 6 + 2), where factor A plots (with and without dead cover) and factor B six herbicides mixture: flumioxazin + S-metolachlor (50.4 + 1,008 g a.i. ha-1), flumioxazin + imazethapyr (60 + 127.2 g a.i. ha-1), pyroxasulfone + sulfentrazone (137.6 + 160 g a.i. ha-1), diuron + sulfentrazone (400 + 200 g a.i. ha-1), metribuzin + S-metolachlor (326.4 + 1,344 g a.i. ha-1) and sulfentrazone + imazethapyr (200 + 100 g a.i. ha-1) and two untreated control plots. As for the results, the herbicides flumioxazin + S-metolachlor, flumioxazin + imazethapyr and pyroxasulfone + sulfentrazone showed excellent control (97-99%) and were not influenced by the plot with and without dead cover. They also showed higher yield soybeans (<2,244 kg ha-1). All herbicides were selective to the soybeans. Overall, pre-emergence herbicides and cover crops were efficient methods for the control of A. hybridus, which farmers should use to avoid losses in yield soybeans due to weed competition.","PeriodicalId":15720,"journal":{"name":"Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes","volume":" ","pages":"497-506"},"PeriodicalIF":1.4,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141492232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantification of cresols in liquid smoke samples employing liquid-liquid extraction with low-temperature purification and analysis by gas chromatography-mass spectrometry. 利用液液萃取和低温净化以及气相色谱-质谱分析法对液体烟雾样本中的甲酚进行定量。

IF 1.4 4区农林科学

Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes Pub Date : 2024-01-01 Epub Date: 2024-07-29 DOI: 10.1080/03601234.2024.2384717

Marina Ranna Pinheiro Vasconcelos, Lucélio Marques Alves Costa, Flaviano Oliveira Silvério, Gevany Paulino de Pinho

{"title":"Quantification of cresols in liquid smoke samples employing liquid-liquid extraction with low-temperature purification and analysis by gas chromatography-mass spectrometry.","authors":"Marina Ranna Pinheiro Vasconcelos, Lucélio Marques Alves Costa, Flaviano Oliveira Silvério, Gevany Paulino de Pinho","doi":"10.1080/03601234.2024.2384717","DOIUrl":"10.1080/03601234.2024.2384717","url":null,"abstract":"Liquid smoke is a food additive and cresols are among its chemical constituents, potentially toxic to human health. Thus, the objective of this study was to develop a method to quantify cresols in liquid smoke. First, the liquid-liquid extraction with low temperature purification (LLE-LTP) was validated for cresols in water, as there are no cresol-free liquid smoke samples. Analyzes were performed by gas chromatography coupled to mass spectrometry in full scan mode. LLE-LTP was subsequently applied in five commercial samples of liquid smoke. Validation results showed that the proposed extraction method was selective for cresols, linear in the range of 0.5 to 35 mg L-1, limit of quantification of 0.5 mg L-1, recovery rate between 90% and 104% and relative standard deviation lower than 10%. The quantification of cresols in liquid smoke samples ranged from 3.0 to 38.3 mg L-1 and the concentration of these chemical contaminants in liquid smoke remained constant for at least 21 days at 25 °C.","PeriodicalId":15720,"journal":{"name":"Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes","volume":" ","pages":"562-570"},"PeriodicalIF":1.4,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Herbicides in the initial growth and chlorophyll indices of sugarcane in pre-sprouted seedlings. 除草剂对甘蔗发芽前幼苗的初始生长和叶绿素指数的影响。

IF 2 4区农林科学

Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes Pub Date : 2024-01-01 Epub Date: 2024-03-25 DOI: 10.1080/03601234.2024.2331946

Ana Ligia Giraldeli, André Felipe Moreira Silva, Gustavo Soares da Silva, Lucas da Silva Araújo, Luísa Carolina Baccin, Giovani Apolari Ghirardello, Ricardo Victoria Filho

{"title":"Herbicides in the initial growth and chlorophyll indices of sugarcane in pre-sprouted seedlings.","authors":"Ana Ligia Giraldeli, André Felipe Moreira Silva, Gustavo Soares da Silva, Lucas da Silva Araújo, Luísa Carolina Baccin, Giovani Apolari Ghirardello, Ricardo Victoria Filho","doi":"10.1080/03601234.2024.2331946","DOIUrl":"10.1080/03601234.2024.2331946","url":null,"abstract":"The aim of the present study was to assess the selectivity of herbicides applied in the pre and post-planting of pre-sprouted seedlings (PSS). The experiment was conducted in a greenhouse, using a completely randomized design, with nine treatments and four repetitions. The IACSP95-5000 cultivar was used. The treatments consisted of herbicides applied in five doses as follows: in pre-planting: sulfentrazone, diclosulam, imazapic, and imazapyr, in post-planting: ethoxysulfuron, halosulfuron, 2,4-D and MSMA, and a control treatment without herbicide. Injury symptoms were assessed at 7, 15, 30, 45, 60, 75, and 90 days after application (DAA), height, diameter, number of tillers, chlorophyll A, B, and total chlorophyll at 30, 60, and 90 DAA, leaf area, shoot and root dry mass at 90 DAA. The post-emergent herbicides were considered selective. Pre-planting herbicides reduced the variables assessed at 90 DAA, observed by linear regression for diclosulam and imazapic, and exponential regression for imazapyr. Treatments with sulfentrazone caused few injury symptoms, with subsequent recovery. In addition, all the variables analyzed were equal to control, with the sulfentrazone considered selective for the IACSP95-5000 cultivar.","PeriodicalId":15720,"journal":{"name":"Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes","volume":" ","pages":"223-232"},"PeriodicalIF":2.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycoremediation of pretilachlor and its metabolite by Aspergillus ficuum. 烟曲霉对苯甲草胺及其代谢物的菌修复。

IF 2 4区农林科学

Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes Pub Date : 2023-01-01 DOI: 10.1080/03601234.2023.2232276

Nancy Kwatra, Jayanthi Abraham

{"title":"Mycoremediation of pretilachlor and its metabolite by Aspergillus ficuum.","authors":"Nancy Kwatra, Jayanthi Abraham","doi":"10.1080/03601234.2023.2232276","DOIUrl":"https://doi.org/10.1080/03601234.2023.2232276","url":null,"abstract":"Pretilachlor is one of the widely used chloroacetamide herbicides in Asian countries to control weeds in the rice field. The extensive use of herbicides has caused major concern among scientists throughout the world. Therefore, it is essential to develop an efficient method for the remediation of pretilachlor and its harmful by-products from contaminated surfaces. Mycoremediation is known to play a key role in the removal of various environmental contaminants. Hence, in the present study, strain AJN2 Aspergillus ficuum was isolated from a paddy field that was in continuous exposure to pretilachlor for over a decade. The degradation studies showed that the strain was efficiently able to degrade 73% of pretilachlor in an aqueous medium within 15 days of incubation and 70% of its major metabolite PME (2-methyl-6-ethylalanine). The GC/MS profile revealed the formation of aldehyde as the end product of degradation which was confirmed through the infrared fingerprint of the degradation sample. The ligninolytic enzyme activity studies showed that the lignin peroxidase enzyme system could be responsible for the degradation of pretilachlor and its major metabolite. The results highlight that the strain AJN2 A. ficuum could be a potential strain for the bioremediation of pretilachlor from the contaminated areas.","PeriodicalId":15720,"journal":{"name":"Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes","volume":"58 6","pages":"489-499"},"PeriodicalIF":2.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10207299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of tolfenpyrad residues in green tea by GC-MS/MS based on acetonitrile extractant, dispersion solid phase extraction purification. 乙腈萃取-分散固相萃取纯化-气相色谱-质谱联用法测定绿茶中甲苯吡虫啉残留量。

IF 2 4区农林科学

Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes Pub Date : 2023-01-01 DOI: 10.1080/03601234.2023.2235249

Songmei Li, Changming Nie

{"title":"Determination of tolfenpyrad residues in green tea by GC-MS/MS based on acetonitrile extractant, dispersion solid phase extraction purification.","authors":"Songmei Li, Changming Nie","doi":"10.1080/03601234.2023.2235249","DOIUrl":"https://doi.org/10.1080/03601234.2023.2235249","url":null,"abstract":"Green tea is one of people's favorite drinks. However, pesticide residues in green tea can cause harm to the human body, and therefore, detection of pesticide residues in green tea is very important. In recent years, the detection of pesticide residues in tea has become a research hotspot. In this paper, a gas chromatography-mass spectrometry/mass spectrometry (GC-MS/MS) detection method of tolfenpyrad pesticide residues in green tea was established by using acetonitrile extractant, dispersive solid-phase extraction purification, temperature programming and application retention time lock with the database. After the sample was extracted with acetonitrile, then the sample was purified by QuEChERS extraction purification tube, afterward isomer B was used as the internal standard for the determination by multiple reaction monitoring mode (MRM) of GC-MS/MS. The results indicated that the experimental data accorded with the criterion on quality control of laboratoris(chemical testing of food), and the requirements of recovery, calibration curve, precision.This method was used to detect tolfenpyrad residues in actual green tea samples in multiple batches, and the satisfactory results were obtained.","PeriodicalId":15720,"journal":{"name":"Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes","volume":"58 7","pages":"515-520"},"PeriodicalIF":2.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10299368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acute exposure of Isopyrazam damages the developed cardiovascular system of zebrafish (Danio rerio). 急性暴露于异吡嗪会损害斑马鱼发达的心血管系统。

IF 2 4区农林科学

Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes Pub Date : 2023-01-01 DOI: 10.1080/03601234.2023.2197655

Yuepei Yan, Shuang Liang, Tao Zhang, Chengchen Deng, Huili Li, Dechuan Zhang, Daoxi Lei, Guixue Wang

{"title":"Acute exposure of Isopyrazam damages the developed cardiovascular system of zebrafish (Danio rerio).","authors":"Yuepei Yan, Shuang Liang, Tao Zhang, Chengchen Deng, Huili Li, Dechuan Zhang, Daoxi Lei, Guixue Wang","doi":"10.1080/03601234.2023.2197655","DOIUrl":"https://doi.org/10.1080/03601234.2023.2197655","url":null,"abstract":"Isopyrazam (IPZ) is one of the broad-spectrum succinate dehydrogenase inhibitor fungicides (SDHIs). Although the potential bio-toxicity of SDHIs has been reported hourly, the specific effects focused on the cardiovascular system have remained unclear and piecemeal. Thus, we chose IPZ as a representative to observe the cardiovascular toxicity of SDHIs in zebrafish. Two types of transgenic zebrafish, Tg (cmlc2:GFP) and Tg (flk1:GFP) were used in this study. Healthy embryos at 6 hpf were exposed to IPZ solutions. The statistical data including survival rate, hatching rate, malformed rate, and morphological and functional parameters of the cardiovascular system at 48 hpf and 72 hpf demonstrated that IPZ could cause abnormalities and cardiovascular defects such as spinal curvature, dysmotility, pericardial edema, pericardial hemorrhage, and slowed heart rate, etc. At the same time, the activity of enzymes related to oxidative stress was altered with IPZ. Our results revealed that IPZ-induced cardiovascular toxicity and oxidative stress might be one of the underlying toxic mechanisms.","PeriodicalId":15720,"journal":{"name":"Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes","volume":"58 4","pages":"367-377"},"PeriodicalIF":2.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9678485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of heavy metal stress in the adaptation strategies of Tulipa luanica growing on serpentine soil through some biomarkers in comparison to Tulipa kosovarica. 通过一些生物标志物评估在蛇纹石土壤上生长的突丽帕与科索沃突丽帕适应策略中的重金属胁迫。

IF 2 4区农林科学

Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes Pub Date : 2023-01-01 Epub Date: 2023-11-02 DOI: 10.1080/03601234.2023.2274743

Mirsade Osmani, Bekim Gashi, Isa R Elezaj, Metin Tuna

{"title":"Assessment of heavy metal stress in the adaptation strategies of Tulipa luanica growing on serpentine soil through some biomarkers in comparison to Tulipa kosovarica.","authors":"Mirsade Osmani, Bekim Gashi, Isa R Elezaj, Metin Tuna","doi":"10.1080/03601234.2023.2274743","DOIUrl":"10.1080/03601234.2023.2274743","url":null,"abstract":"The aim of this study was to gain a better understanding of how Tulipa luanica adapts to growth in soil with higher concentrations of heavy metals and to assess potential toxic effects using various biomarkers, in comparison to Tulipa kosovarica, a typical serpentine species. For this purpose, we analyzed the concentrations of Al, Ca, Cd, Co, Cr, Cu, Fe, Mg, Mn, Ni, Pb, and Zn in the soil, as well as their accumulation in plants and their associated stress effects. The results indicate that, despite the presence of some metals in very high concentrations in the soil (Al, Fe, Mn, and Ni), they are translocated in minimal amounts within plant organs, particularly in T. luanica. Nearly all metals exhibited significantly higher concentrations in T. kosovarica when compared to T. luanica. Based on the analysis of biomarkers, it is apparent that T. luanica shows greater sensitivity to these conditions. This is evident through the decreased activity of δ-aminolevulinic acid dehydratase and levels of δ-aminolevulinic acid, malondialdehyde, and glutathione observed in T. luanica. It appears that T. luanica effectively restricts the absorption of metals in serpentine soils; however, it experiences oxidative stress induced by these metals, setting it apart from the more resilient T. kosovarica.","PeriodicalId":15720,"journal":{"name":"Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes","volume":" ","pages":"718-725"},"PeriodicalIF":2.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66783923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of optical density-based growth kinetics for pure culture Campylobacter jejuni, coli and lari grown in blood-free Bolton broth. 在无血Bolton肉汤中生长的纯培养空肠弯曲杆菌、大肠杆菌和拉里菌基于光密度的生长动力学的比较。

IF 2 4区农林科学

Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes Pub Date : 2023-01-01 Epub Date: 2023-10-12 DOI: 10.1080/03601234.2023.2264742

Aaron R Bodie, Michael J Rothrock, Steven C Ricke

{"title":"Comparison of optical density-based growth kinetics for pure culture Campylobacter jejuni, coli and lari grown in blood-free Bolton broth.","authors":"Aaron R Bodie, Michael J Rothrock, Steven C Ricke","doi":"10.1080/03601234.2023.2264742","DOIUrl":"10.1080/03601234.2023.2264742","url":null,"abstract":"Campylobacter growth kinetic parameters can be used to refine the sensitivity and efficiency of microbial growth-based methods. Therefore, the aim of this study was to construct growth curves for C. jejuni, C. coli, and C. lari in pure culture and calculate growth kinetics for each Campylobacter species in the same environmental conditions. Campylobacter jejuni, C. coli and C. lari were grown over 48 h and inoculated into 15 mL Hungate tubes (N = 3 trials per species; 5 biological replicates per trial; 3 species; 1 strain per species). Absorbance measurements were taken in 45 min intervals over 24 h. Optical density readings were plotted versus time to calculate growth kinetic parameters. C. jejuni exhibited the longest lag phase (p < 0.001) at 15 h 20 min ± 30 min, versus C. coli at 11 h 15 min ± 17 min, and C. lari at 9 h 27 min ± 15 min. The exponential phase duration was no longer than 5 h for all species, and doubling times were all less than 1h 30 min. The variation in growth kinetics for the three species of Campylobacter illustrates the importance of determining individual Campylobacter spp. growth responses for optimizing detection based on low bacterial levels. This study provides kinetics and estimates to define enrichment times necessary for low concentration Campylobacter detection.","PeriodicalId":15720,"journal":{"name":"Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes","volume":" ","pages":"671-678"},"PeriodicalIF":2.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41132453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0