Journal of Evidence-based Integrative Medicine最新文献

{"title":"Characterization of Antibiofilm and Antimicrobial Effects of <i>Trigona</i> Stingless Bee Honey Compared to Stinging Bee <i>Centaurea hyalolepis</i> and <i>Citrus</i> Honeys.","authors":"Walid Salem Aburayyan, Nesrin Seder, O'la Al-Fawares, AbdulFattah Fararjeh, Ibrahim S Majali, Yousef Al-Hajaya","doi":"10.1177/2515690X241271978","DOIUrl":"10.1177/2515690X241271978","url":null,"abstract":"<p><p>The antibiofilm and antimicrobial properties of tropical honey types including Malaysian stingless bee honey remain explicitly unexplored when compared with Apies honey. The antibiofilm and antimicrobial activities of the Malaysian <i>Trigona</i> honey were characterized with two stinging bee honey types (<i>Centaurea hyalolepis</i> and <i>Citrus</i> honeys) from Jordan. The antibiofilm and antimicrobial investigations were conducted on a set of seven microbial strains; five bacterial species of <i>Pseudomonas aeruginosa</i> ATCC 10145<i>, Streptococcus pyogenes</i> ATCC 19615<i>, Staphylococcus aureus</i> ATCC 25923, <i>Escherichia coli</i> ATCC 25922, <i>Klebsiella pneumoniae</i> ATCC <i>13883,</i> and two fungal strains <i>Candida albicans</i> ATCC 10231 <i>and Candida krusei</i> ATCC 14243. The antimicrobial investigations revealed a broad spectrum activity for <i>Trigona</i> honey against Gram-positive, Gram-negative, and fungal strains over the two honey types. One-way ANOVA showed a significant difference (<i>p < 0.001</i>) in the zone of inhibition ranging from 9 to 25 mm and minimum inhibition activity (MIC) ranged from 9.4-29.6% (w/v) against the microbial strains. Moreover, the addition of honey to established biofilms has induced a degradation activity in the biofilm mass. Two-way ANOVA showed a significant biofilm degradation proportion (<i>p < 0.001</i>) ranging from 1.3% to 91.3% following treatment with <i>Trigona</i> honey and the other honey types in relevance to the concentration ranging from 10% to 50% (w/v). Moreover, the antibiofilm activity was highly consistent with MIC affecting bacterial growth inhibition. In conclusion, a robust antimicrobial and antibiofilm activity for <i>Trigona</i> stingless bee honey over the stinging bee <i>Centaurea hyalolepis</i> and <i>Citrus</i> honeys is noticed which endows the usage of <i>Trigona</i> honey in the antimicrobial industry.</p>","PeriodicalId":15714,"journal":{"name":"Journal of Evidence-based Integrative Medicine","volume":"29 ","pages":"2515690X241271978"},"PeriodicalIF":3.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11311187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141906775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Garcinia dulcis</i> Extract Alleviates Inflammation in Kidney and Liver of the 2-Kidney-1-Clip Hypertensive rat.","authors":"Nattaya Thongsepee, Wanwisa Himakhun, Ratsikan Parachat, Pongsakorn Martviset, Pathanin Chantree, Phornphan Sornchuer, Kant Sangpairoj, Siriphun Hiranyachattada","doi":"10.1177/2515690X241244845","DOIUrl":"https://doi.org/10.1177/2515690X241244845","url":null,"abstract":"<p><p><i>Garcinia dulcis</i> (GD) extract possesses anti-hypertensive property that are poorly characterized. This study aimed to investigate an anti-inflammatory effect of GD flower extract in the 2-kidney-1-clip (2K1C) hypertensive compared to sham operative (SO) rat. Male Wistar rats were divided into 2 groups; the 2K1C group in which a silver clip was placed around renal artery to induce hypertension, and the SO normotensive group. Four weeks later, each group of rats were further divided into 2 subgroups, each subgroup was orally gavaged of either corn oil (vehicle) or 50 mg/kg BW GD extract daily for 4 weeks. The malondialdehyde (MDA) levels in serum, liver, and kidney were determined. Hematoxylin and eosin staining was carried out for histological examination, Periodic acid - Schiff staining for glomerular injury, Masson's trichrome staining for renal fibrosis, and immunohistochemistry for either tumor necrosis factor alpha (TNF-α) or endothelial nitric oxide synthase (eNOS) investigation. Taken together, our results demonstrated that GD flower extract decreased the MDA level in both serum and liver and kidney tissue and suppressed the expression of TNF-α in both liver and kidney of 2K1C hypertensive rats. Mesangial cell proliferation, expansion of mesangial matrix, widening Bowman's capsule space, congestion of glomerular capillary and vessel, cloudy swelling of renal tubular epithelial cell, and renal fibrosis were observed in the kidneys of 2K1C rats. Therefore, we concluded that GD flower extract can alleviate liver and kidney inflammation in which partially attenuates the glomerular injury in the 2K1C rat.</p>","PeriodicalId":15714,"journal":{"name":"Journal of Evidence-based Integrative Medicine","volume":"29 ","pages":"2515690X241244845"},"PeriodicalIF":3.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11016236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140853157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Aqueous Extract of Unripe <i>Musa Paradisiaca</i> Linn on Parameters Affecting Reproduction in Rats.","authors":"Collins Azubuike Onyeto, Akachukwu Marytheresa Onwuka, Ikechukwu Emmanuel Peter, Chukwuemeka Sylvester Nworu, Peter Achunike Akah","doi":"10.1177/2515690X241249534","DOIUrl":"https://doi.org/10.1177/2515690X241249534","url":null,"abstract":"<p><strong>Objective: </strong>Aqueous extract of unripe <i>Musa paradisiaca</i> fruit is commonly used for the treatment of ulcers in eastern Nigeria. This study aimed to assess the acute and subacute effects of an aqueous extract of unripe fruit on male and female fertility in rats.</p><p><strong>Methods: </strong>Aqueous extracts obtained by maceration were analyzed for acute and subacute toxicity and for the presence of phytochemical constituents using standard procedures. The extract (100, 500, and 1000 mg/kg) was administered daily to rats of both sexes for 28 d. Blood samples collected on days 0 and 28 were assessed for follicle-stimulating hormone (FSH), luteinizing hormone (LH), catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA). Testes and ovaries were harvested for histopathological analysis. Sperm were also collected to determine the sperm count and motility.</p><p><strong>Results: </strong>Phytochemical screening revealed the presence of saponins, tannins, alkaloids, and resins. After an oral dose of up to 5000 mg/kg, there were no deaths in the acute toxicity test. The extract (500 mg/kg) significantly (<i>P </i>< .05) enhanced sperm count and motility relative to the untreated control; significantly (<i>P </i>< .05) reduced SOD, CAT, and glutathione levels, while significantly (<i>P </i>< .05) elevated LH, FSH, and MDA levels in male and female rats. Histological examination revealed significant structural damage to the ovaries.</p><p><strong>Conclusion: </strong>Unripe <i>Musa paradisiaca</i> fruit exhibited an adverse toxicological profile following prolonged administration and caused oxidative stress in rodents.</p>","PeriodicalId":15714,"journal":{"name":"Journal of Evidence-based Integrative Medicine","volume":"29 ","pages":"2515690X241249534"},"PeriodicalIF":3.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11057342/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140860326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Best Practice Recommendations for Conducting and Reporting Controlled Trials in Clinical Hypnosis Research.","authors":"Zoltan Kekecs, Donald Moss, Peter J Whorwell, Katalin Varga, Devin B Terhune, Philip D Shenefelt, Olafur S Palsson, Giuseppe De Benedittis, Gary Elkins","doi":"10.1177/2515690X241274538","DOIUrl":"https://doi.org/10.1177/2515690X241274538","url":null,"abstract":"<p><p>There is an abundance of outcomes research for clinical hypnosis showing promising results. Nonetheless, hypnosis is still underutilized in clinical care. For a behavioral intervention to enter mainstream clinical care, efficacy needs to be demonstrated with exceptionally high quality of evidence, and its reporting needs to be complete and sufficiently clear to enable replication and clinical use. The present article provides best practice guidelines formulated by the <i>Task Force for Establishing Efficacy Standards for Clinical Hypnosis</i> for conducting and reporting clinical hypnosis research.The recommendations are presented in two tiers. Tier I recommendations include essential best practices, such as a call for the use of detailed research and intervention manuals, plans for and reporting of participant-education about hypnosis, the use of hypnotizability scales with good psychometric properties, and clear reporting of the hypnotizability measurement. Tier I also includes the sharing of intervention manuals, the reporting of the induction procedure, the labeling of the intervention for participants, and the definition of hypnosis used. Tier II includes preferred recommendations, calling for measurement of adherence to home practice, measurement of hypnotizability using scales with both subjective and behavioral measures of responsiveness, and the involvement of participants from the full hypnotizability spectrum. Tier II also includes the assessment of variables related to proposed mechanisms of action, the reporting of participants prior hypnosis experiences, and the relationship of expectancies and treatment outcomes.This list of recommendations will be useful for researchers, reviewers, and journal editors alike when conducting, reporting, or evaluating studies involving clinical hypnosis.</p>","PeriodicalId":15714,"journal":{"name":"Journal of Evidence-based Integrative Medicine","volume":"29 ","pages":"2515690X241274538"},"PeriodicalIF":3.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11483803/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alkalinization Using Sodium Bicarbonate for COVID-19 Treatment: A Systematic Review and Meta-Analysis.","authors":"Arman Shafiee, Kyana Jafarabady, Hassan Moltazemi, Mohammad Javad Amini, Mohammad Ali Rafiei, Amirhossein Akhondi, Sayed-Hamidreza Mozhgani","doi":"10.1177/2515690X241258403","DOIUrl":"10.1177/2515690X241258403","url":null,"abstract":"<p><strong>Background: </strong>A systematic review and meta-analysis have been conducted to evaluate the efficacy of alkalinization for COVID-19 patients based on current evidence to determine the impact of alkalinization on COVID-19 outcomes.</p><p><strong>Methods: </strong>We searched MEDLINE (Pubmed), Web of Science, Cochrane Library, and Clinicaltrials.gov for studies evaluating the efficacy of alkalinization up to 30 April 2023. Based on the PRISMA 2020 statement criteria a systematic review and meta-analysis of studies were performed.</p><p><strong>Results: </strong>The results of our meta-analysis showed a significant reduction in mortality rate in the alkalinization group compared to controls (RR 0.73, 95% CI: 0.56-0.95; I2  =  0%). However, our subgroup analysis showed no significant improvement in RCT-only studies (RR 0.78, 95% CI: 0.59-1.05; I2  =  0%), the recovery rate was significantly higher in the alkalinization group (RR 2.13, 95% CI: 1.39-3.26; I2  =  0%), duration of recovery also has improved in alkalinization group (SMD 0.76, 95% CI: 0.33-1.18; I2  =  0%). The results of our meta-analysis showed a significant reduction in the duration of hospitalization in the alkalinization group compared to controls with very low certainty of evidence (SMD -0.66, 95% CI: -0.97 to -0.35; I2  =  36%).</p><p><strong>Conclusion: </strong>With low certainty of evidence, alkalinization (by sodium bicarbonate) can be an efficient and safe adjuvant treatment for COVID-19 patients. Future randomized controlled trials are needed to strengthen the available evidence.</p>","PeriodicalId":15714,"journal":{"name":"Journal of Evidence-based Integrative Medicine","volume":"29 ","pages":"2515690X241258403"},"PeriodicalIF":3.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11145993/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aesthetic Radiofrequency Associated with <i>Rosmarinus officinalis</i> Supplementation is Safe and Reduces Oxidative Stress in Women: Randomized, and Double-Blind Clinical Trial.","authors":"Greissi Tatieli Franke Tremêa, Karine Raquel Uhdich Kleibert, Lenara Schalanski Krause, Ana Paula Weber Fell, Anais Regina Scapini, Keli Wilchen Marschall, Cristiano Sartori Baiotto, Martha Héllen Tremêa da Silva, José Antonio Gonzalez da Silva, Christiane de Fátima Colet","doi":"10.1177/2515690X241246293","DOIUrl":"10.1177/2515690X241246293","url":null,"abstract":"<p><p>The objective were to evaluate the effects of supplementation of standardized dry extract of Rosmarinus officinalis (RO) and the application of aesthetic radiofrequency on the oxidative stress markers catalase (CAT), superoxide dismutase (SOD), non-protein thiols (NP-SH), and thiobarbituric acid reactive species (TBARS) and the biochemical markers triglycerides, total cholesterol, high density lipoprotein (HDL) cholesterol, glutamic-oxaloacetic transaminase (TGO/AST), pyruvic-glutamic transaminase (TGP/ALT), gamma glutamyl transpeptidase (gamma-GT), and creatinine. This study included 32 women received the aesthetic therapy to reduce localized fat. They were divided into the control group (n = 8) receiving placebo capsules and the intervention group (n = 24) subdivided into Group A, B, and C, each with eight members receiving supplementation with 100, 500, and 1000 mg/day of standardized dry extract of RO, respectively. The Universal Trial Number (UTN) - U1111-1274-6255. Supplementation with RO (500 mg/day) demonstrated a reduction in oxidative stress (quantified with through a significant increase in NP-SH and a reduction in SOD and CAT enzymes). The radiofrequency aesthetic treatment did not promote an increase in oxidative stress; however, it caused significant changes in total cholesterol, HDL cholesterol, and creatinine. RO is a plant with antioxidant effects and its oral consumption is safe in selected women subjects in hepatic and renal markers.</p>","PeriodicalId":15714,"journal":{"name":"Journal of Evidence-based Integrative Medicine","volume":"29 ","pages":"2515690X241246293"},"PeriodicalIF":3.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11320689/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141971234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Objective Evaluation of Pulse Width Using an Array Pulse Diagram.","authors":"Zi-Juan Bi, Ji Cui, Xing-Hua Yao, Xiao-Juan Hu, Si-Han Wang, Meng-Chen Liang, Zhi-Hui Zhou, Jia-Tuo Xu","doi":"10.1177/2515690X241241859","DOIUrl":"10.1177/2515690X241241859","url":null,"abstract":"<p><strong>Background: </strong>Pulse width, which can reflect <i>qi</i>, <i>blood</i> excess, and deficiency, has been used for diagnosing diseases and determining the prognosis in traditional Chinese medicine (TCM). This study aimed to devise an objective method to measure the pulse width based on an array pulse diagram for objective diagnosis.</p><p><strong>Methods: </strong>The channel 6, the region wherein the pulse wave signal is the strongest, is located in the middle of the pulse sensor array and at the <i>guan</i> position of <i>cunkou</i> during data collection. Therefore, the main wave (h₁) time of the pulse wave was collected from the channel 6 through calculation. The left h₁ time was collected from the remaining 11 channels. The amplitudes at these time points were extracted as the h₁ amplitudes for each channel. However, the pulse width could not be calculated accurately at 12 points. Consequently, a bioharmonic spline interpolation algorithm was used to interpolate the h₁ amplitude data obtained from the horizontal and vertical points, yielding 651 (31 × 21) h₁ amplitude data. The 651 data points were converted into a heat map to intuitively calculate the pulse width. The pulse width was calculated by multiplying the number of grids on the vertical axis with the unit length of the grid. The pulse width was determined by TCM doctors to verify the pulse width measurement accuracy. Meanwhile, a color Doppler ultrasound examination of the volunteers' radial arteries was performed and the intravascular meridian widths of the radial artery compared with the calculated pulse widths to determine the reliability.</p><p><strong>Results: </strong>The pulse width determined using the maximal h₁ amplitude method was comparable with the radial artery intravascular meridian widths measured using color Doppler ultrasound. The h₁ amplitude was higher in the high blood pressure group and the pulse width was greater.</p><p><strong>Conclusions: </strong>The pulse width determined using the maximal h₁ amplitude was objective and accurate. Comparison between the pulse widths of the normal and high blood pressure groups verified the reliability of the method.</p>","PeriodicalId":15714,"journal":{"name":"Journal of Evidence-based Integrative Medicine","volume":"29 ","pages":"2515690X241241859"},"PeriodicalIF":3.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11119526/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140305821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Phytolacca Dodecandra</i> (L' Herit) (Phytolaccaceae) Methanol Root Extract Protects Liver from Acetaminophen-Induced Injury in Rats.","authors":"Gudeta Duga Geresu, Tilahun Tesfaye Abebe, Diriba Alemayehu Gadisa, Getu Melese Taye, Dejene Hailu Bedada, Tamirat Bekele Baressa, Shemsu Umer Hussen, Tesemma Sileshi Chala","doi":"10.1177/2515690X241263063","DOIUrl":"10.1177/2515690X241263063","url":null,"abstract":"<p><p><i>Phytolacca dodecandra</i> (L' Herit), or 'Endod', is one of the widely known medicinal plants in Ethiopia. Berries of the endod have been used as a detergent for centuries. The present study was aimed to test the hepatoprotective effects of the plant against acetaminophen (APAP)-induced liver injury in rats. Mice of either sex were used for oral acute toxicity tests and APAP-induced lethality tests. Hepatoprotective experiments were done on male rats using 2 g/kg of APAP to induce liver damage. Liver enzymes, total bilirubin (TB), and lipid profile were determined. Liver tissues were also examined histopathologically to see a morphologic change in the control and experiment groups. The protective effect of the plant extract was also tested through sodium pentobarbital (SPB)-induced sleeping time. A significant increase in serum levels of liver enzymes, TB, low-density lipoprotein (LDL), and triglycerides (TGs) was seen from oral administration of 2 g/kg APAP. Total cholesterol (TC) and high-density lipoprotein (HDL) levels were decreased. Serum levels of all parameters were reversed to normal after administration of silymarin 100 mg/kg and, 100, 200, and 400 mg/kg doses of the extract. A significant dose-dependent hepatoprotective effect of <i>Phytolacca dodecandra</i> Methanol Root Extract (PDME) was seen in terms of LDL. Histopathological investigations and SPB-induced sleeping time confirmed the findings of biochemical analysis. The findings of the present study indicate that PDME protected the liver from APAP injury.</p>","PeriodicalId":15714,"journal":{"name":"Journal of Evidence-based Integrative Medicine","volume":"29 ","pages":"2515690X241263063"},"PeriodicalIF":3.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11273601/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melanogenesis Inhibitory Activity, Chemical Components and Molecular Docking Studies of <i>Prunus cerasoides</i> Buch.-Ham. D. Don. Flowers.","authors":"Nateelak Kooltheat,&nbsp;Aman Tedasen,&nbsp;Kenshi Yamasaki,&nbsp;Moragot Chatatikun","doi":"10.1177/2515690X231152928","DOIUrl":"https://doi.org/10.1177/2515690X231152928","url":null,"abstract":"<p><p>Safe depigmenting agents are currently increasing in the cosmetic or pharmaceutical industry because various compounds have been found to have undesirable side effects. Therefore, the present study aimed to investigate the melanogenesis inhibitory effects of <i>Prunus cerasoides</i> Buch. -Ham. D. Don. flower extracts and their molecular mechanism in B16F10 mouse melanoma cells. Moreover, we also examined phenolic and flavonoid contents, antioxidant activity, chemical constituents of potential extracts, and molecular docking. The highest phenolic and flavonoid contents with the greatest scavenging activity were found in the butanol extract of the <i>P. cerasoides</i> flower compared to other extracts. From all extracts, only crude, diethyl ether, and butanol extracts showed an inhibition of mushroom tyrosinase activity, cellular tyrosinase activity, and melanin content as well as the downregulation of the gene expression of the microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein-1 (TRP-1), and tyrosinase-related protein-2 (TRP-2) in α-MSH-stimulated B16F10 cells. Based on the molecular docking study, n-hexadecanoic acid, heptadecanoic acid, octadecanoic acid, 9,12-octadecadienoic acid, 9,12,15-octadecanoic acid, and eicosanoic acid might show an inhibitory effect against tyrosinase and MITF. In conclusion, this finding demonstrates that both the diethyl ether and butanol extracts of the <i>P. cerasoides</i> flower can effectively reduce tyrosinase activity and melanin synthesis through the downregulation of the melanogenic gene expression in B16F10 cells and through the molecular docking study. Taken together, the diethyl ether and butanol extracts of the <i>P. cerasoides</i> flower could be an anti-melanogenic ingredient for hyperpigmentary or melasma treatment.</p>","PeriodicalId":15714,"journal":{"name":"Journal of Evidence-based Integrative Medicine","volume":"28 ","pages":"2515690X231152928"},"PeriodicalIF":3.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/45/35/10.1177_2515690X231152928.PMC9905211.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9237947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Antimalarial Activity of Ethanolic Extract of <i>Annona muricata L</i>.: An <i>in vivo</i> and an <i>in silico</i> Approach.","authors":"Charles Obiora Nwonuma,&nbsp;Elizabeth Abiodun Balogun,&nbsp;Gideon Ampoma Gyebi","doi":"10.1177/2515690X231165104","DOIUrl":"https://doi.org/10.1177/2515690X231165104","url":null,"abstract":"<p><p>In Nigeria, <i>Annona muricata</i> L. has been used to treat a variety of ailments. The mechanism of the antimalarial activity of ethanolic leaf extract of <i>Annona muricata</i> (EEAML) was investigated using both an in vivo and an in silico approach. The experimental mice were divided into five groups: A-F. The mice in groups B-F were inoculated with <i>Plasmodium berghei</i> NK-65 and treated accordingly. Groups A and B are the negative and positive controls (infected and untreated), respectively. Group C received 10 mg/kg chloroquine (standard drug), whereas groups D-F received 100, 200, and 300 mg/kg body weight of the extract orally respectively. The mice were euthanized eight days after infection, and their liver and blood were collected and used in biochemical tests. Molecular docking was performed using the extract's HPLC compounds and <i>Plasmodium falciparum</i> proteins. In the suppressive, prophylactic, and curative tests, there was a significant decrease (p < 0.05) in parasitemia levels in groups treated with the extract compared to the positive control and standard drug. When compared to the positive control, there was a significant (p < 0.05) reduction in liver MDA, total cholesterol, and total triglyceride levels. The binding energies of luteolin and apigenin-<i>pf</i>protein complexes were significantly (p < 0.05) higher compared to their respective references. The anti-plasmodial activity of the extract may result from its hypolipidemic effect, which deprives the parasite of essential lipid molecules needed for parasite growth, as well as from the inhibitory effects of apigenin and luteolin on specific proteins required for the <i>Plasmodium</i> metabolic pathway.</p>","PeriodicalId":15714,"journal":{"name":"Journal of Evidence-based Integrative Medicine","volume":"28 ","pages":"2515690X231165104"},"PeriodicalIF":3.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/25/56/10.1177_2515690X231165104.PMC10084581.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9997074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}