Journal of Experimental Zoology最新文献_第3页

Collagen fibrils are differently organized in weight-bearing and not-weight-bearing regions of pig articular cartilage. 胶原原纤维在猪关节软骨负重区和非负重区组织结构不同。

Journal of Experimental Zoology Pub Date : 2000-10-01

S Gomez, R Toffanin, S Bernstorff, M Romanello, H Amenitsch, M Rappolt, R Rizzo, F Vittur

{"title":"Collagen fibrils are differently organized in weight-bearing and not-weight-bearing regions of pig articular cartilage.","authors":"S Gomez, R Toffanin, S Bernstorff, M Romanello, H Amenitsch, M Rappolt, R Rizzo, F Vittur","doi":"","DOIUrl":"","url":null,"abstract":"The magnetic resonance (MR) appearance of the weight-bearing (\"loaded\") and not-weight-bearing (\"unloaded\") regions in T(2)-weighted images of pig articular cartilage is different. On the hypothesis that this difference may be ascribed, at least in part, to a different collagen fibre organization in the two regions, this organization was studied using biochemical, histological, and X-ray diffraction methods. While the mean concentrations of collagen and of its cross-links were the same in the two regions, a regular small angle X-ray diffraction pattern was observed only for the habitually \"loaded\" tissue. It was also seen by light microscopy that the four typical functional zones were well displayed in the \"loaded\" cartilage whereas they were not clearly depicted in the \"unloaded\" tissue. Collagen presented a high concentration of fibrils forming an intricate and dense meshwork at the surface of both \"loaded\" and \"unloaded\" cartilage. A second zone of high collagen concentration was present at the upper layer of the deep zone of \"loaded\" cartilage. By contrast, this lamina of highly concentrated fibrils was lacking in \"unloaded\" cartilage and collagen fibrils appear thinner. Our study proves that the organization of collagen fibres is different for the \"loaded\" and \"unloaded\" regions of articular cartilage. It also suggests that this different organization may influence the MR appearance of the tissue. J. Exp. Zool. 287:346-352, 2000.","PeriodicalId":15686,"journal":{"name":"Journal of Experimental Zoology","volume":"287 5","pages":"346-52"},"PeriodicalIF":0.0,"publicationDate":"2000-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21816667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of bovine seminal ribonuclease and bovine pancreatic ribonuclease A on bovine oocyte maturation. 牛精核糖核酸酶和牛胰核糖核酸酶A对牛卵母细胞成熟的影响。

Journal of Experimental Zoology Pub Date : 2000-10-01

T Slavík, J Matousek, J Fulka, R T Raines

{"title":"Effect of bovine seminal ribonuclease and bovine pancreatic ribonuclease A on bovine oocyte maturation.","authors":"T Slavík, J Matousek, J Fulka, R T Raines","doi":"","DOIUrl":"","url":null,"abstract":"Bovine seminal ribonuclease (BS-RNase) contains the MxM (noncovalent dimer) and M=M (free monomer) in constant ratio. The aim of this work was to evaluate the effect of BS-RNase, its monomer and dimer forms, and also various mutants of this enzyme on meiotic completion in cattle oocytes. It was found that BS-RNase has irreversible effects on the meiotic maturation of bovine oocytes in vitro, particularly on the completion of meiosis. The effect of BS-RNase is dose-dependent. In medium supplemented with 1 microg/ml, the results were comparable with those of the control (70% MII oocytes after 24 hr of culture). Whereas 5 microg/ml reduced the number of MII oocytes to 50%, 10 and 25 microg/ml arrested this process completely. The MxM form and RNase A at 5 microg/ml inhibited the maturation rate by 71 and 48%, respectively, but a less significant effect was observed for the M=M form, or the carboxymethylated monomers MCM31 and MCM32 (21%, 16%, and 42% MII oocytes, respectively, in comparison with control). These data demonstrate that bovine ribonucleases can have variable detrimental effects on the maturation of bovine oocyte. J. Exp. Zool. 287:394-399, 2000.","PeriodicalId":15686,"journal":{"name":"Journal of Experimental Zoology","volume":"287 5","pages":"394-9"},"PeriodicalIF":0.0,"publicationDate":"2000-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21816671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Collagen study and regulation of the de novo synthesis by IGF-I in hemocytes from the gastropod mollusc, Haliotis tuberculata. 胃足类软体动物haaliotis tuberculata血细胞中igf - 1新生合成的胶原蛋白研究和调控。

Journal of Experimental Zoology Pub Date : 2000-09-01

A Serpentini, C Ghayor, J M Poncet, V Hebert, P Galéra, J P Pujol, E Boucaud-Camou, J M Lebel

{"title":"Collagen study and regulation of the de novo synthesis by IGF-I in hemocytes from the gastropod mollusc, Haliotis tuberculata.","authors":"A Serpentini, C Ghayor, J M Poncet, V Hebert, P Galéra, J P Pujol, E Boucaud-Camou, J M Lebel","doi":"","DOIUrl":"","url":null,"abstract":"To evidence a collagen synthesis and identify which type(s) of collagen is present in hemocytes from the mollusc Haliotis tuberculata, we have performed three separate approaches, namely, de novo synthesis by cultured cells, immunological approaches, and northern blot analysis. We demonstrated first that after 40-hr labeling, the de novo synthesis of collagen in the cell layer of cultured hemocytes represents 9.48 +/- 1.25% with respect to the total [(3)H]proline-labeled protein synthesis. In addition, IGF-I elicited a significant stimulation of collagen synthesis in cultured hemocytes in a dose-dependent manner from 10(-10) to 10(-8) M. The maximal stimulation (10(-9) M) induced an increase of 286 +/- 56% with respect to 100% control. By immunocytochemistry and immunoblotting, we showed that hemocytes present immunoreactive molecules to antibodies directed against the type I fibrillar collagen. In addition, using as a probe Hf 677 corresponding to a human pro alpha1(I) collagen cDNA and which encompasses the (Gly-X-Y) repeated sequence found in all Metazoa, four collagen transcripts of approximately 6.4, 5, 2.2, and 2 kb in length have been detected. These data suggest the presence of fibrillar type I collagen in hemocytes and are compatible with the concept that these cells are involved in the extracellular matrix deposition, a cardinal function in tissue repair as well as in developmental processes. Our model may appear as an excellent system to study the role of growth factors on the regulation of collagen synthesis by molluscan hemocytes. J. Exp. Zool. 287:275-284, 2000.","PeriodicalId":15686,"journal":{"name":"Journal of Experimental Zoology","volume":"287 4","pages":"275-84"},"PeriodicalIF":0.0,"publicationDate":"2000-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21788049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Introduction of a foreign gene into medakafish using the particle gun method. 用粒子枪法将外源基因导入三角鱼。

Journal of Experimental Zoology Pub Date : 2000-09-01

M Yamauchi, M Kinoshita, M Sasanuma, S Tsuji, M Terada, M Morimyo, Y Ishikawa

引用次数: 0

Identification of maturation-inducing steroid in a freshwater perch Anabas testudineus and differential responses of intact follicles and denuded oocytes to cyclic AMP in oocyte maturation. 淡水鲈鱼成熟诱导类固醇的鉴定及卵母细胞成熟过程中完整卵泡和脱毛卵母细胞对环AMP的差异反应。

Journal of Experimental Zoology Pub Date : 2000-09-01

S Bhattacharyya, U Sen, S P Bhattacharyya, D Mukherjee

{"title":"Identification of maturation-inducing steroid in a freshwater perch Anabas testudineus and differential responses of intact follicles and denuded oocytes to cyclic AMP in oocyte maturation.","authors":"S Bhattacharyya, U Sen, S P Bhattacharyya, D Mukherjee","doi":"","DOIUrl":"","url":null,"abstract":"Postvitellogenic follicles of freshwater perch Anabas testudineus incubated with [(3)H]pregnenolone as exogenous precursor produced several metabolites, including 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one (DHP) and 5 beta-pregnane-3 alpha, 17 alpha,20 beta-triol (5 beta-3 alpha,17 alpha,20 beta-P). These were identified by chromatography, microchemical reactions, and crystallization to constant specific activity. Following stimulation with fish (perch) pituitary extract (FPE) there was significant high production of DHP and 5 beta-3 alpha,17 alpha,20 beta-P, concomitant with a high percentage of germinal vesicle breakdown (GVBD). Inhibitor of steroidogenesis (trilostane) and inhibitors of protein synthesis (cycloheximide and actinomycin-D) completely blocked FPE-induced pregnenolone metabolism and oocyte maturation. The effectiveness of various C(21) steroids in inducing GVBD was examined. Results indicate that DHP was the most potent inducer of GVBD than other structurally related C(21) steroids. In intact follicles, FPE-stimulated production of DHP was shown to be mediated through the adenylate cyclase-cAMP pathway. Addition of IBMX or forskolin, which increases the endogenous cAMP level, as well as directly supplementing dbcAMP to the incubation medium, had no inhibitory effect on DHP-induced GVBD in the intact follicles. But all these agents were shown to inhibit GVBD in fully denuded oocytes. This study provides evidence that DHP, produced by postvitellogenic follicles through the adenylate cyclase-cAMP pathway, is the maturation-inducing steroid in freshwater perch and that the role played by cAMP in the induction of GVBD in intact follicles is different from that in the denuded oocytes. J. Exp. Zool. 287:294-303, 2000.","PeriodicalId":15686,"journal":{"name":"Journal of Experimental Zoology","volume":"287 4","pages":"294-303"},"PeriodicalIF":0.0,"publicationDate":"2000-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21788051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructure and motility of the caudal epididymis spermatozoa from the volcano mouse (Neotomodon alstoni alstoni Merriam, 1898). 火山小鼠尾侧附睾精子的超微结构和运动(Neotomodon alstoni alstoni Merriam, 1898)。

Journal of Experimental Zoology Pub Date : 2000-09-01

I Villalpando, H Villafan-Monroy, D Aguayo, A Zepeda-Rodriguez, H G Espitia, A Chavez-Olivares

{"title":"Ultrastructure and motility of the caudal epididymis spermatozoa from the volcano mouse (Neotomodon alstoni alstoni Merriam, 1898).","authors":"I Villalpando, H Villafan-Monroy, D Aguayo, A Zepeda-Rodriguez, H G Espitia, A Chavez-Olivares","doi":"","DOIUrl":"","url":null,"abstract":"The volcano mouse Neotomodon alstoni alstoni is a genus endemic to the higher elevations of the Mexican transvolcanic belt. In the present study we examined for the first time the morphological features of the spermatozoa taken from the caudal epididymis of this species by transmission and scanning electron microscopy. Spermatozoan motility was studied in sucrose and bicarbonate solutions; vitality and morphology were observed by light microscopy. Transmission electron microscopy shows that the head of spermatozoon is asymmetric and possesses a large and curved hook. The axoneme of the spermatozoan tail is highly developed at fibers 1, 5, and 6. Absolute and relative measurements of the length of the head, the midpiece, and the rest of the tail were also obtained. N. alstoni alstoni spermatozoa were hyperactive in the presence of 290 mM sucrose and 10 and 20 nM bicarbonate solutions exhibited high motility (180-190 microm/sec), and high flagellum beating frequency (10-12 Hz). In contrast, the spermatozoa in 310 mM sucrose solution showed scarce motility (13.5 +/- 3.8 microm/sec) and low beating frequency (1.5 +/- 0.4 Hz). It is proposed that the volcano mouse spermatozoa possess some features very similar to other mammalian spermatozoa and that bicarbonate triggers caudal epididymal sperm motility of this species. J. Exp. Zool. 287:316-326, 2000.","PeriodicalId":15686,"journal":{"name":"Journal of Experimental Zoology","volume":"287 4","pages":"316-26"},"PeriodicalIF":0.0,"publicationDate":"2000-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21788053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid activation of gill Na(+),K(+)-ATPase in the euryhaline teleost Fundulus heteroclitus. 宽盐硬骨鱼鳃Na(+)、K(+)- atp酶的快速激活。

Journal of Experimental Zoology Pub Date : 2000-09-01

J M Mancera, S D McCormick

{"title":"Rapid activation of gill Na(+),K(+)-ATPase in the euryhaline teleost Fundulus heteroclitus.","authors":"J M Mancera, S D McCormick","doi":"","DOIUrl":"","url":null,"abstract":"The rapid activation of gill Na(+),K(+)-ATPase was analyzed in the mummichog (Fundulus heteroclitus) and Atlantic salmon (Salmo salar) transferred from low salinity (0.1 ppt) to high salinity (25-35 ppt). In parr and presmolt, Salmo salar gill Na(+),K(+)-ATPase activity started to increase 3 days after transfer. Exposure of Fundulus heteroclitus to 35 ppt seawater (SW) induced a rise in gill Na(+), K(+)-ATPase activity 3 hr after transfer. After 12 hr, the values dropped to initial levels but showed a second significant increase 3 days after transfer. The absence of detergent in the enzyme assay resulted in lower values of gill Na(+),K(+)-ATPase, and the rapid increase after transfer to SW was not observed. Na(+),K(+)-ATPase activity of gill filaments in vitro for 3 hr increased proportionally to the osmolality of the culture medium (600 mosm/kg > 500 mosm/kg > 300 mosm/kg). Osmolality of 800 mosm/kg resulted in lower gill Na(+),K(+)-ATPase activity relative to 600 mosm/kg. Increasing medium osmolality to 600 mosm/kg with mannitol also increased gill Na(+),K(+)-ATPase. Cycloheximide inhibited the increase in gill Na(+),K(+)-ATPase activity observed in hyperosmotic medium in a dose-dependent manner (10(-4) M > 10(-5) M > 10(-6) M). Actinomycin D or bumetanide in the culture (doses of 10(-4) M, 10(-5) M, and 10(-6) M) did not affect gill Na(+),K(+)-ATPase. Injection of fish with actinomycin D prior to gill organ culture, however, prevented the increase in gill Na(+),K(+)-ATPase activity in hyperosmotic media. The results show a very rapid and transitory increase in gill Na(+),K(+)-ATPase activity in the first hours after the transfer of Fundulus heteroclitus to SW that is dependent on translational and transcriptional processes. J. Exp. Zool. 287:263-274, 2000.","PeriodicalId":15686,"journal":{"name":"Journal of Experimental Zoology","volume":"287 4","pages":"263-74"},"PeriodicalIF":0.0,"publicationDate":"2000-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21788048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activation of porcine oocytes using cyclopiazonic acid, an inhibitor of calcium-dependent ATPases. 使用钙依赖性atp酶抑制剂环吡唑酸激活猪卵母细胞。

Journal of Experimental Zoology Pub Date : 2000-09-01

J Petr, J Rozinek, F Jílek, D Urbánková

{"title":"Activation of porcine oocytes using cyclopiazonic acid, an inhibitor of calcium-dependent ATPases.","authors":"J Petr, J Rozinek, F Jílek, D Urbánková","doi":"","DOIUrl":"","url":null,"abstract":"Cyclopiazonic acid (CPA), a potent inhibitor of endogenous calcium-dependent ATPases, is able to induce parthenogenetic activation in pig oocytes matured in vitro. Sixty-four percent of matured pig eggs cultured with 100 nM CPA for 4 hr were activated. A similar activation rate was observed in oocytes treated with thapsigargin, another inhibitor of calcium-dependent ATPases. The parthenogenetic development of CPA-activated eggs did not proceed beyond the 8-cell stage. The blockage of calcium channels by verapamil only slightly decreased the proportion of CPA-activated pig oocytes. This indicates that the release of calcium from intracellular stores is sufficient for oocyte activation and calcium influx from extracellular sources has no significant role. The significant decrease in CPA-activated oocytes (100 nM of CPA for 4 hr) after a microinjection of heparin indicated that the mobilization of intracellular calcium stores is mediated through inositol trisphosphate receptors. On the other hand, the only slightly depressed activation rate in oocytes microinjected with ruthenium red and procaine indicates that CPA mobilizes a much smaller amount of calcium through the ryanodine receptors. The marked inhibitory effect of ophiobolin A and W7 on the activation of CPA-treated pig oocytes suggests that the calcium signal, as the second messenger, acts downstream through calmodulin. J. Exp. Zool. 287:304-315, 2000.","PeriodicalId":15686,"journal":{"name":"Journal of Experimental Zoology","volume":"287 4","pages":"304-15"},"PeriodicalIF":0.0,"publicationDate":"2000-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21788052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hodin J. 2000. Plasticity and constraints in development and evolution. J exp zool (Mol dev evol) 288:1-20 霍丁J. 2000。发展和演变中的可塑性和约束。[J] exp zool (Mol dev evolution) 28:1-20

Journal of Experimental Zoology Pub Date : 2000-08-15

Kourakis, Martindale

引用次数: 0

Phylogenetic analysis of the Wnt gene family and discovery of an arthropod wnt-10 orthologue. Wnt基因家族的系统发育分析及节肢动物Wnt -10同源物的发现。

Journal of Experimental Zoology Pub Date : 2000-08-15

E L Jockusch, K A Ober

引用次数: 0