Journal of Dairy Research最新文献

{"title":"Thistle-curdled cheeses: exploring consumer acceptance and willingness to pay in the Mediterranean.","authors":"Serena Mandolesi, Emel Ozturk, Simona Naspetti, Raffaele Zanoli","doi":"10.1017/S0022029926102398","DOIUrl":"https://doi.org/10.1017/S0022029926102398","url":null,"abstract":"<p><p>Vegetable rennet (VR) has a long history in Mediterranean cheese-making, and in response to the growing demand for vegetarian-friendly cheeses, enzymes extracted from the thistle <i>Cynara humilis</i> have become a promising substitute for calf rennet. This study investigated consumer acceptance and willingness to pay (WTP) for artisanal cheeses produced with thistle rennet in three Mediterranean countries: Italy, Spain and Tunisia. Sensory evaluations focused on feta cheese in all three countries, while queso de Murcia al vino was additionally tested in Italy and Spain. The research was conducted in three stages. First, focus groups explored consumers' knowledge, beliefs and attitudes towards thistle rennet. Second, hedonic tests were carried out under three conditions: blind liking (tasting without information), expected liking (only information, no tasting) and actual liking (tasting with information). Finally, an experimental auction was conducted for feta cheese to assess consumers' WTP. In general, consumers showed positive perceptions of thistle-curdled cheeses, despite some cross-country differences. Both intrinsic factors (e.g., taste and fat content) and extrinsic cues (e.g., information, price and origin) influenced acceptance. Overall, results highlight the potential of VR for traditional cheeses, with specific emphasis on targeted marketing strategies and transparent labelling to support their adoption as high-value artisanal products.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-11"},"PeriodicalIF":1.2,"publicationDate":"2026-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148150115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of a novel experimental NanoQuil vaccine against <i>Staphylococcus aureus-</i>induced mastitis.","authors":"Claudia Lützelschwab, Caroline Fossum, Josef Dahlberg","doi":"10.1017/S0022029926102428","DOIUrl":"https://doi.org/10.1017/S0022029926102428","url":null,"abstract":"<p><p>Despite implementing preventive measures, mastitis caused by <i>Staphylococcus aureus</i> remains a significant challenge in Swedish dairy farming, and an efficient vaccine against mastitis is still sought. In this research article, results from a clinical trial evaluating the efficacy of a novel experimental vaccine against <i>S. aureus</i>-induced mastitis in a Swedish dairy herd with continuous calving are presented. The vaccine was formulated with NanoQuil adjuvant and formalin-fixed <i>S. aureus</i> antigen. The trial was conducted as a comparative study in which previously unvaccinated cows were vaccinated before calving with either the experimental vaccine or a commercially available control vaccine (Startvac). Milk yield and somatic cell count (SCC) were the primary evaluation criteria. Data from the Swedish Official Milk Recording Scheme were used in the analysis of infection rates and herd udder health. In total, data from 144 cows across 199 lactation periods were evaluated. The two vaccine groups did not exhibit any significant differences in milk yield, and there were only minor, inconsistent differences in SCC between the treatments. However, the overall herd udder health evidently improved after the introduction of the control and treatment vaccines. No difference in the rate of new infections, as indicated by SCC increases above a threshold of 150 000 cells/ml between samplings, was recorded between the experimental groups. In contrast, the rate of new infections during the dry period tended to be lower. The recovery rate tended to be higher for cows vaccinated with the experimental vaccine, although not statistically significant.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-8"},"PeriodicalIF":1.2,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147987767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methionine promotes cell proliferation in bovine mammary epithelial cells via the ATF4-GSK3β signalling pathway.","authors":"Yanying Zhang, Yalin Xie, Meiyan Liu, Fengxian Sui, Jing Gao, Jiadi Su, Yang Yang, Ye Lin, Xiaoming Hou, Na Zhang","doi":"10.1017/S0022029926102416","DOIUrl":"https://doi.org/10.1017/S0022029926102416","url":null,"abstract":"<p><p>Increasing the methionine (Met) supply to lactating cows will increase mammary cell proliferation. Activating transcription factor 4 (ATF4) is a regulator of cellular amino acid sensing, linking nutrient signals to cellular anabolism; however, its role in regulating bovine mammary epithelial cell (BMEC) proliferation remains largely unknown. In this study, ATF4 expression paralleled that of Cyclin D1 in mammary tissues from dairy cows at different developmental stages (puberty, lactation and dry period), suggesting that ATF4 is associated with cell proliferation. In BMECs, knockdown of ATF4 increased the number of cells arrested in the G1 phase (∼11%; <i>P</i> < 0.05; control) and downregulated Cyclin D1 expression (∼40%; <i>P</i> < 0.05; control), indicating that ATF4 is required for cell proliferation. Treatment of BMECs with increasing concentrations of Met (0, 0.3, 0.6, 0.9, 1.0 and 1.2 mM) showed that 0.6 mM Met enhanced mammalian target of rapamycin (mTOR) phosphorylation and increased protein expression of ATF4 and Cyclin D1, without activating canonical endoplasmic reticulum stress markers. Inhibition assays demonstrated that mTOR is required for Met-induced ATF4 activation. Functional studies further revealed that ATF4 is a key mediator of Met-regulated cell proliferation. Mechanistically, Met facilitated the physical interaction between ATF4 and glycogen synthase kinase-3β (GSK3β), promoting GSK3β phosphorylation at Ser9 and subsequently increasing Cyclin D1 expression. Collectively, these findings indicate that Met promotes BMEC proliferation through the mTOR-ATF4-GSK3β signalling pathway, providing a potential nutritional strategy to enhance mammary cell proliferation in dairy cows.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-12"},"PeriodicalIF":1.2,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147987716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research communication: comparison of calf faeces and rumen digesta as inoculum to measure <i>in vitro</i> digestibility using the gas production technique.","authors":"Stefan Yerby, James Huntington, Nicholas N Jonsson, Helen Warren","doi":"10.1017/S0022029926102337","DOIUrl":"https://doi.org/10.1017/S0022029926102337","url":null,"abstract":"<p><p>This Research Communication addresses the hypothesis that calf faeces can be used as a microbial inoculum in the <i>in vitro</i> gas production (GP) technique to model rumen digestibility in calves. <i>In vitro</i> GP is a valuable method of screening dietary interventions in ruminants. However, the technique requires a microbial inoculum, typically obtained from the rumens of fistulated animals. Using faecal inocula that can be collected non-invasively would be ethically and economically preferable, if it provided equivalent results. The aim of this pilot study was to compare <i>in vitro</i> GP from faecal and ruminal inocula obtained from dairy calves that were killed either pre-weaning or post-weaning. Ruminal content and faeces were collected from calves aged 4, 7 and 13 weeks old, and incubated with calf starter concentrates in the ANKOM^RF system. A weak relationship between inocula was seen for all modelled GP kinetic parameters (R² < 0.53), although modelling indicated that incubations using faecal and rumen inocula from pre-weaned calves had similar lag times and rates of degradation. In weaned calves, aged 13 weeks, faecal incubations produced less gas, lag times were longer, and the rate of degradation of the slowly degradable fraction of calf starter was slower, compared to rumen incubations. Weak relationships, and kinetic differences, between rumen and faecal inocula would likely hinder the use of faeces to model calf rumen fermentation.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-5"},"PeriodicalIF":1.2,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: <i>Journal of Dairy Research</i> instructions for peer reviewers.","authors":"Nicholas N Jonsson","doi":"10.1017/S0022029926102386","DOIUrl":"https://doi.org/10.1017/S0022029926102386","url":null,"abstract":"","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"1-4"},"PeriodicalIF":1.2,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycerophospholipids in dairy cow health and longevity: a review.","authors":"David Bruce Sheedy, Helen Marie Golder, Sergio Carlos Garcia, Ian John Lean","doi":"10.1017/S0022029926102222","DOIUrl":"10.1017/S0022029926102222","url":null,"abstract":"<p><p>This review examines the role glycerophospholipids (<b>PL</b>) in dairy cow health, with specific focus on phosphatidylcholine (<b>PC</b>), phosphatidylethanolamine (<b>PE</b>), phosphatidylinositol (<b>PI</b>) and phosphatidylserine (<b>PS</b>). Increasing parity of cows is associated with lower concentrations of plasma PL that contain very long-chain omega-3 fatty acids, including docosahexaenoic acid and eicosapentaenoic acid, which are precursors for prostaglandin synthesis, and have anti-inflammatory roles. Low concentrations of these PL could plausibly contribute to the increased risk of disease, reproductive failure and mortality in older cows. The bioavailability and metabolism of fatty acids may differ among supplements that are predominately neutral lipids, such as triacylglycerol-rich oils, and those bound to PL including pasture, whole or ground oilseeds and fish meal. Hepatic lipidosis can occur during the transition period if there is insufficient very-low density lipoproteins (<b>VLDL</b>) production in the liver to transport lipids into blood circulation. The PC are the primary PL of VLDL and are produced by two main pathways in the liver, the cytidine diphosphate-choline pathway that uses choline as a substrate, and the PE <i>N</i>-methyltransferase pathway that uses PE and methyl-donors as substrates. Co-supplementation strategies that target both pathways may increase PC production over a one-pathway supplementation strategy. The PIs are phosphoinositides precursors, which have broad physiological roles including regulating inflammatory processes and may offer targets for novel treatment and management of disease. Both the PI and PE are precursors to endocannabinoids, important regulators of energy metabolism, immune function and reproduction in mammals. Early findings on the endocannabinoid system in transition dairy cows yielded results that diverge from non-ruminant models. The PS expression on cytoplasmic membranes signals apoptosis, coagulation and contributes to sperm-oocyte recognition. As lipidomic diagnostics become increasingly available, understanding the metabolism of PL will continue to develop and promises to offer novel strategies for optimising cattle health and longevity.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"223-237"},"PeriodicalIF":1.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147512470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic epidemiology, antimicrobial resistance and risk-tier stewardship of dairy <i>Staphylococcus aureus</i> from bulk-tank milk in subtropical China.","authors":"Shuanghui Zhou, Dejiang Fu, Peng Meng, Yonghui Huang, Tao Han","doi":"10.1017/S0022029926102106","DOIUrl":"10.1017/S0022029926102106","url":null,"abstract":"<p><p>Bovine mastitis caused by <i>Staphylococcus aureus</i> increases antimicrobial use and may compromise milk quality, yet genomic evidence from subtropical China remains limited. This study aimed to characterise the genomic epidemiology, antimicrobial non-susceptibility and functional risk tiers of dairy-associated <i>S. aureus</i> in northern Fujian, China. We analysed 59 <i>S. aureus</i> isolates recovered from 162 bulk-tank milk samples collected from 14 farms during 2023-2024, using whole-genome sequencing, MLST/<i>spa</i> typing and core-genome SNP phylogenetics, together with disc diffusion susceptibility testing (CLSI M100) and annotation of resistance, virulence and plasmid replicons (CARD, VFDB and PlasmidFinder). An unsupervised multivariate workflow (principal component analysis followed by K-means clustering) integrated genomic and phenotypic features to assign functional-risk tiers. Seven MLST and eight <i>spa</i> types were detected, dominated by ST1-t114/t127 (40.7%) and ST9784-t189 (20.3%). Livestock-associated MRSA ST59-t437 carrying SCCmec IV and IEC-D was detected in raw milk from this region for the first time. Overall, 45.8% of isolates were non-susceptible to at least one antimicrobial, most commonly penicillin and ampicillin (28.8% each). ST9784-t189 more frequently carried <i>lnu(A)</i> and pLNU4-like plasmid sequences and showed higher clindamycin non-susceptibility than other lineages. PCA/K-means grouped isolates into low-, medium- and high-risk tiers (76.3%, 15.3% and 8.4%, respectively); medium/high tiers showed higher multidrug non-susceptibility and a greater enterotoxin gene burden and were more often associated with plasmid replicons including pKH1 and pAMalpha1. This single-province pilot study provides a genomic baseline for dairy-associated <i>S. aureus</i> in subtropical China and suggests that unsupervised multivariate grouping may support risk-aware surveillance and milk-quality assurance; larger multi-region datasets are needed to assess generalisability.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"274-284"},"PeriodicalIF":1.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147390265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knowledge, attitudes, and perceptions toward dairy fat and consumption of dairy fat in Mexico, Chile, and Argentina - Erratum.","authors":"Einar Vargas-Bello-Pérez, Paula Toro-Mujica, América Chávez Martínez, Karen Tajonar, Andrew Ainslie, Juan Pablo Keim, Javier Baudracco, Navid Ghavipanje","doi":"10.1017/S0022029925101623","DOIUrl":"10.1017/S0022029925101623","url":null,"abstract":"","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"199"},"PeriodicalIF":1.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147271168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of water adulteration in camel milk by freezing point.","authors":"Gaukhar Konuspayeva, Moldir Nurseitova, Elizaveta Chuvashova, Zauresh Bilal, Farida Amutova, Bernard Faye","doi":"10.1017/S002202992610212X","DOIUrl":"10.1017/S002202992610212X","url":null,"abstract":"<p><p>This Research Communication aimed to establish reference freezing point (FP) values for raw Bactrian and dromedary camel milk and to evaluate the detectability of water dilution up to 30% using the cryoscopy method. A total of 38 milk samples from healthy camels in Kazakhstan were analysed. Pure Bactrian camel milk exhibited FP values between -0.542°C and -0.799°C, while dromedary milk ranged from -0.527°C to -0.655°C. Progressive dilution caused a significant linear increase in FP in both species (<i>p</i> < 0.0001). Although a 10% water addition resulted in measurable changes, natural variability may mask slight adulteration. FP showed weak correlations with protein and pH, with species-specific differences. These results provide reliable reference values for the FP and confirm the possibility of detecting adulteration of camel milk.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"321-324"},"PeriodicalIF":1.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147627874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of the use of ozonated water in sanitization and prevention of mastitis in dairy cows.","authors":"Jamile Achy, Esther Abihail Fuentes, Larissa M Dos Santos, José Cabral de S Neto, Claudio Cézar Dos Santos Freire, Amanda C G Graboschii, Thereza Cristina Bório Dos Santos Calmon Bittencourt, Tobyas Maya de Albuquerque Mariz, Juliana de Oliveira Bernardo, Pierre Barnabé Escodro","doi":"10.1017/S0022029926102155","DOIUrl":"10.1017/S0022029926102155","url":null,"abstract":"<p><p>Applying teat dips to cows' teats before and after milking (pre- and post-dipping) are important methods for preventing mastitis. Given the proven effectiveness of ozonated water for eliminating bacterial colonies, this study aimed to evaluate the effectiveness of ozonated water in preventing mastitis in pre- and post-dipping of dairy cows. Fifty-eight cows were selected based on negative black-bottom cup test results and the California mastitis test (CMT), and randomly allocated into two groups: the control group (CG, <i>n</i> = 23) where pre- and post-dipping were done with a commercial hydrogen peroxide product, and the ozone group (OG3, <i>n</i> = 35) where ozonated water at a concentration of 2 µg/ml was used, prepared immediately before use with an ozone generator. Samples were collected at four timepoints: M0 (pre), M1 (72 h), M2 (7 d) and M3 (14 d). Somatic cell count, CMT, pH, milk composition, and microbiological identification were analysed. The black-bottom cup test and physical evaluation of the udder were performed daily before milking. All milk composition variables were similar in both groups. SCC remained within normal ranges for both groups at all time points as per the guidelines of the Brazilian Ministry of Agriculture, Livestock and Food Supply. Microbiological cultures revealed similar pathogens across time points with the same growth dynamics. The study demonstrated that the use of ozonated water was as effective as the commercial product used in pre- and post-dipping for hygiene and prevention of mastitis in dairy cows, potentially serving as a viable alternative with lower environmental contamination, especially on organic dairy farms. Further studies comparing different concentrations and evaluations against other sanitizers are necessary.</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":" ","pages":"268-273"},"PeriodicalIF":1.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147512494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}