Journal of Dental Sciences最新文献

{"title":"Furcation-involved class II subclass B molar treated by Er:YAG laser-assisted comprehensive periodontal pocket therapy (Er-LCPT) under a microscope: A case report","authors":"Taichen Lin, Min Yee Ng, Yoichi Taniguchi, Akira Aoki","doi":"10.1016/j.jds.2025.08.017","DOIUrl":"10.1016/j.jds.2025.08.017","url":null,"abstract":"","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 1","pages":"Pages 629-631"},"PeriodicalIF":3.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145903881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rethinking English medium instruction for dental education in Taiwan","authors":"Ching-Shuen Wang , Feng-Chou Cheng , Wei-Chun Lin, Chun-Pin Chiang","doi":"10.1016/j.jds.2025.10.013","DOIUrl":"10.1016/j.jds.2025.10.013","url":null,"abstract":"","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 1","pages":"Pages 665-667"},"PeriodicalIF":3.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145903890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pleomorphic adenoma with extensive squamous metaplasia - A case report","authors":"Hung-Pin Lin, Chun-Pin Chiang","doi":"10.1016/j.jds.2025.10.016","DOIUrl":"10.1016/j.jds.2025.10.016","url":null,"abstract":"","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 1","pages":"Pages 668-670"},"PeriodicalIF":3.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145903891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zoledronate enhances lipopolysaccharide-induced inflammation via toll-like receptor 4 upregulation","authors":"Kanan Bando ,&nbsp;Yasuo Endo","doi":"10.1016/j.jds.2025.06.004","DOIUrl":"10.1016/j.jds.2025.06.004","url":null,"abstract":"<div><h3>Background/Purpose</h3><div>Bisphosphonates (BPs) are commonly used to treat osteoporosis and bone metastases, but their use is associated with bisphosphonate-related osteonecrosis of the jaw (BRONJ), a condition characterized by chronic inflammation and impaired healing. Since bacterial infection is a major risk factor for BRONJ, this study investigated whether zoledronate (Zol), a nitrogen-containing BP, modulates Toll-like receptor 4 (TLR4) expression and enhances lipopolysaccharide (LPS)-induced inflammation.</div></div><div><h3>Materials and methods</h3><div>Zol or other BPs were injected into the ear pinnae of mice. Five days later, tissues were harvested and analyzed by flow cytometry and ELISA to assess TLR4 expression and cytokine production. TLR4 expression in immune cells and keratinocytes was evaluated, and inflammatory cytokine production (IL-1β, TNF-α) was measured after LPS stimulation.</div></div><div><h3>Results</h3><div>TLR4 expression was significantly increased in keratinocytes and neutrophils after Zol administration, whereas it was decreased in macrophages due to an increased proportion of inflammatory macrophage subsets with intrinsically low TLR4 expression. Zol-treated neutrophils exhibited elevated IL-1β and TNF-α production following LPS stimulation. Co-administration of Zol and LPS markedly enhanced local inflammation compared to either agent alone.</div></div><div><h3>Conclusion</h3><div>Zoledronate upregulates TLR4 expression in neutrophils and keratinocytes and augments LPS-induced inflammation, suggesting a mechanism by which BPs exacerbate oral inflammatory responses. These findings provide new insight into the pathogenesis of BRONJ and suggest that targeting TLR4 signaling may be a potential strategy to mitigate BP-associated oral complications.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 1","pages":"Pages 184-190"},"PeriodicalIF":3.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145904003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of multimodal large language models on visually-based questions in the Japanese National Examination for Dental Hygienists: A preliminary comparative study","authors":"Yoshino Kaneyasu ,&nbsp;Yuichi Mine ,&nbsp;Yoshie Niitani ,&nbsp;Tsuyoshi Taji ,&nbsp;Saori Takeda ,&nbsp;Ryohei Tokinaga ,&nbsp;Hideo Shigeishi ,&nbsp;Toshinobu Takemoto ,&nbsp;Naoya Kakimoto ,&nbsp;Takeshi Murayama ,&nbsp;Kouji Ohta","doi":"10.1016/j.jds.2025.06.005","DOIUrl":"10.1016/j.jds.2025.06.005","url":null,"abstract":"<div><h3>Background/purpose</h3><div>The importance of oral health is globally recognized, which has increased the demand for qualified dental hygienists. This study assessed the performance of multimodal large language models (LLMs), on the Japanese National Examination for Dental Hygienists, focusing on their ability to answer visually-based questions and evaluating image-recognition capabilities.</div></div><div><h3>Materials and methods</h3><div>The 34th Japanese National Examination for Dental Hygienists (March 2025) supplied 213 multiple-choice questions (74 text-only, 139 visually-based). Five multimodal LLMs were tested: OpenAI o3-mini-high (o3-mh), ChatGPT-4.5 Preview (GPT-4.5), Gemini 2.0 Flash Thinking Experimental (Gemini 2.0), Gemini 2.5 Pro Experimental (Gemini 2.5), and Claude 3.7 Sonnet (Claude 3.7). Performance was evaluated by comparing LLM answers to official correct answers. Cochran's Q test and McNemar's tests with Bonferroni correction were used for statistical analysis.</div></div><div><h3>Results</h3><div>Gemini 2.5 achieved the highest overall correct response rate (85.0 %), followed by Claude 3.7 (77.5 %), o3-mh (77.0 %), GPT-4.5 (76.1 %), and Gemini 2.0 (75.1 %). For text-only questions, Claude 3.7 (91.9 %) performed best. On visually-based questions, Gemini 2.5 was superior (82.0 %), while other models scored around 70–73 %. Gemini 2.5 significantly outperformed, GPT-4.5 and Gemini 2.0 overall, and GPT-4.5 and Claude 3.7 on visually-based questions.</div></div><div><h3>Conclusion</h3><div>Multimodal LLMs, particularly Gemini 2.5, demonstrate significant proficiency on the Japanese National Examination for Dental Hygienists, including questions with visual elements. These findings suggest a growing potential for LLMs as educational tools in dental hygiene. However, current limitations in accuracy and reliability necessitate further refinement and cautious integration into educational and clinical settings.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 1","pages":"Pages 198-205"},"PeriodicalIF":3.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145904005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The roles of CD24-Sec14 like lipid binding 2 (SEC14L2) axis in the neoplastic progression of oral squamous cell carcinomas","authors":"Shi-Rou Chang ,&nbsp;Chung-Hsien Chou ,&nbsp;Chung-Ji Liu ,&nbsp;Kuo-Wei Chang ,&nbsp;Jian-Hua Pan ,&nbsp;Sheng-Lin Yen ,&nbsp;Shu-Chun Lin","doi":"10.1016/j.jds.2025.08.014","DOIUrl":"10.1016/j.jds.2025.08.014","url":null,"abstract":"<div><h3>Background/purpose</h3><div>Immune stimulation or escape are critical factors determining the survival of malignancies, including oral squamous cell carcinoma (OSCC) and head and neck SCC (HNSCC). CD24 (CD24A in mice) is a glycoprotein anchored to cell membranes, modulating macrophages' immune responses, cell interaction, tumorigenesis, and stemness. However, its roles in the OSCC pathogenesis are still controversial. The modulation of CD24 on the oncogenicity and immunity of OSCC were investigated in this study.</div></div><div><h3>Materials and methods</h3><div>Knockdown approaches and the establishment of stable tet-off CD24 expression cell subclones were used in cell and mouse models for phenotypic and transcriptomic analysis. Bioinformatic assessments were performed to specify the clinicopathological implications.</div></div><div><h3>Results</h3><div>CD24 expression modulated the increase of migration and invasion and the upregulation of phosphatidylcholine/phosphatidylinositol transfer protein Sec14 like lipid binding 2 (SEC14L2) expression. The syngeneic grafts of CD24 tet-off expressing murine OSCC cell subclones exhibited modest changes of immune cell infiltration within tumors and were devoid of immune profile disruption in the recipient's neck lymph node and spleen. The shutdown of CD24 with doxycycline treatment drastically suppressed the growth of CD24 tet-off tumors. A correlation between CD24 expression and myeloid dendritic cell population was noted in murine and human OSCC tissue. Concordances in CD24 and SEC14L2 expression and oncogenic induction were noted in murine tumors. SEC14L2 was upregulated in HNSCC/OSCC tumors, and it was an unfavorable survival predictor.</div></div><div><h3>Conclusion</h3><div>This study's elucidation of the oncogenic potential of the CD24-SEC14L2 axis may signify the therapeutic efficacy of CD24 targeting for HNSCC/OSCC.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 1","pages":"Pages 232-243"},"PeriodicalIF":3.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145904008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trimethyl chitosan: Antibacterial activity on Enterococcus faecalis biofilm and cytocompatibility on human periodontal ligament fibroblasts cells","authors":"Raras Ajeng Enggardipta ,&nbsp;Minato Akizuki ,&nbsp;Mika Bando ,&nbsp;Yuji Inagaki ,&nbsp;Kazumitsu Sekine ,&nbsp;Kenichi Hamada ,&nbsp;Tomoko Sumitomo ,&nbsp;Kanta Sato ,&nbsp;Hiromichi Yumoto","doi":"10.1016/j.jds.2025.06.022","DOIUrl":"10.1016/j.jds.2025.06.022","url":null,"abstract":"<div><h3>Background/purpose</h3><div>Effective disinfection of the root canal system remains a major challenge due to complex anatomy and the persistence of biofilm-forming bacteria such as <em>Enterococcus faecalis</em>. Trimethyl chitosan (TMC), a quaternized chitosan derivative, has shown promising antimicrobial properties but has not been extensively studied for endodontic use. This study aimed to evaluate the antibacterial and antibiofilm efficacy of TMC against <em>E. faecalis</em> and assess its cytocompatibility with human periodontal ligament fibroblasts (HPdLFs).</div></div><div><h3>Materials and methods</h3><div>The <em>E. faecalis</em> biofilm formation in the presence of TMC and antibacterial activity of TMC against mature <em>E. faecalis</em> biofilms were evaluated using crystal violet staining, adenosine triphosphate assays, colony forming unit counting, scanning electron microscopy, and fluorescence microscopy. The expression of genes associated with <em>E. faecalis</em> biofilm formation, such as <em>ace</em>, <em>esp</em>, and <em>gelE,</em> was determined. Moreover, cytocompatibility of TMC with HPdLFs was assessed using a cell counting kit-8 assay.</div></div><div><h3>Results</h3><div>TMC significantly inhibited biofilm formation by <em>E. faecalis</em> and in the mature <em>E. faecalis</em> biofilm, TMC interfered with the total biofilm biomass, reduced bacterial numbers, weakened the biofilm structure, and upregulated <em>ace</em>, <em>esp</em>, and <em>gelE</em> expression. Furthermore, a lower concentration of TMC maintained HPdLFs viability.</div></div><div><h3>Conclusion</h3><div>This study highlights the potential of TMC as a novel irrigating material owing to its antibacterial and antibiofilm activities against <em>E. faecalis</em> and its cytocompatibility with HPdLFs.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 1","pages":"Pages 150-158"},"PeriodicalIF":3.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145903950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Measurement of predictive validity of admission criteria used by public dental colleges in Pakistan. Time to reconsider","authors":"Shahid Akhtar Akhund","doi":"10.1016/j.jds.2025.08.028","DOIUrl":"10.1016/j.jds.2025.08.028","url":null,"abstract":"<div><h3>Background/purpose</h3><div>Dentistry education evolved from treating painful teeth to comprehensive oral care program. Oral health is an integral component of general healthcare. Like medical institutes, dental institutes have introduced admission tests as a component of selection criteria. However, various selection criteria components are not investigated for their predictive validity. This study aimed to measure the predictive value of selection criteria used in Pakistani dental colleges. This study provides recommendations for revision of weightings of admission criteria.</div></div><div><h3>Materials and methods</h3><div>This is a retrospective, quantitative and longitudinal cohort study. Deidentified data from a public dental college of Pakistan were analyzed. The Secondary School Certificate Examination (SSCE), Higher Secondary School Certificate Examination (HSSCE), and admission test scores were used as predictive variables, and professional examinations scores as outcome variables. Spearman's correlation coefficient and multivariate multiple regression (MMR) analyses were performed.</div></div><div><h3>Results</h3><div>Most data were left skewed, suggesting higher scores. The SSCE and admission test scores showed moderate positive correlation with professional examinations (<em>P-</em>values &lt;0.001 to &lt; 0.05). The R² values from 0.439 to 0.343, indicating a moderate to good amount of variance explained for each professional examination. The SSCE and admission test scores make a significant predictive model for all professional examinations.</div></div><div><h3>Conclusion</h3><div>The SSCE and admission test are the significant predictors of successful professional examinations. The Pakistan Medical and Dental Council (PMDC) should reconsider selection criteria weightings. A revised higher weighting for SSCE and admission test and lower weighting for HSSCE scores would bring fairness in the selection process.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 1","pages":"Pages 258-264"},"PeriodicalIF":3.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145904036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long non-coding RNA PP7080 enhances cancer stemness and tumorigenic features in oral cancer","authors":"Chih-Yu Peng ,&nbsp;Yu-Wei Chiu ,&nbsp;Chun-Chung Huang ,&nbsp;Po-Hsuan Tang ,&nbsp;Fang-Yu Chang ,&nbsp;Yi-Wen Liao ,&nbsp;Cheng-Chia Yu ,&nbsp;Shih-Min Wang","doi":"10.1016/j.jds.2025.07.004","DOIUrl":"10.1016/j.jds.2025.07.004","url":null,"abstract":"<div><h3>Background/purpose</h3><div>Emerging evidence has shown that various failures in cancer therapy, such as drug resistance, metastasis, and cancer relapse are attributed to cancer stem cells (CSCs). Also, growing attention has been paid to the regulation of non-coding RNAs in cancer stemness. Here, we aimed to investigate the impact of lncRNA PP7080 on cancer stemness in oral squamous cell carcinomas.</div></div><div><h3>Materials and methods</h3><div>PP7080 expression was assessed in ALDH1⁺ and ALDH1^- cells sorted from oral squamous cell carcinoma cell lines using qRT-PCR. PP7080 was silenced in ALDH1⁺ SAS cells, and the effects on stemness characteristics, including ALDH1 activity, cell migration, colony formation, CD44 and Oct4 expression, and the expression of drug resistance-related markers ABCG2 and Bmi-1, were examined. The interaction between PP7080 and miR-601 was validated using a luciferase reporter assay. Rescue experiments were performed by inhibiting miR-601 in PP7080-silenced cells using apoptosis analysis. PP7080 expression in HNSCC was analyzed using the TCGA-HNSCC database.</div></div><div><h3>Results</h3><div>Our results showed that PP7080 was significantly overexpressed in oral cancer tissues and positively correlated with stemness markers. The phenotypic and flow cytometry assays demonstrated that suppression of PP7080 reduced the migratory and colony-forming aibilities, cancer stemness features, and self-renewal abilities in oral CSCs. Furthermore, we demonstrated that PP7080 may enhance cancer stemness features by counteracting the tumor-suppressive effect of miR-601.</div></div><div><h3>Conclusion</h3><div>Targeting PP7080 may offer a promising therapeutic strategy for oral cancer by reducing cancer stemness.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 1","pages":"Pages 125-132"},"PeriodicalIF":3.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145904038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ovatodiolide overcomes cisplatin resistance in head and neck cancer by disrupting a novel oncogenic signature and cancer-associated fibroblast activation","authors":"Chi-Hsueh Yang ,&nbsp;Rachel Raditya Renantha ,&nbsp;Po-Chih Hsu ,&nbsp;Alexander TH. Wu ,&nbsp;Szu-Yuan Wu ,&nbsp;Nur Azizah ,&nbsp;Ritmaleni Ritmaleni ,&nbsp;Jia-Hong Chen ,&nbsp;Jih-Chin Lee ,&nbsp;Chih-Yuan Fang ,&nbsp;Kuan-Chou Lin","doi":"10.1016/j.jds.2025.08.041","DOIUrl":"10.1016/j.jds.2025.08.041","url":null,"abstract":"<div><h3>Background/purpose</h3><div>Malignant head and neck squamous cell carcinoma (HNSCC) is an aggressive cancer with complex tumor microenvironment interactions. We aimed to identify key molecular drivers and therapeutic targets using bioinformatics approaches and examine their associations with cancer-associated fibroblasts (CAF).</div></div><div><h3>Materials and methods</h3><div>We analyzed three HNSCC transcriptomic datasets using bioinformatics. Protein–protein interaction networks were created using STRING, and pathway enrichment was also performed. The clinical relevance and CAF association of genes were evaluated using the TCGA HNSCC cohort and TIMER 2.0. Molecular docking predicted ovatodiolide binding to target proteins. Bioinformatics findings were validated in HNSCC cell lines and normal fibroblasts (WS1) by assessing cell viability, tumor spheroid formation, and CAF transformation through viability assays, qPCR, and Western blot. A mouse model of cisplatin resistance was used to test ovatodiolide's therapeutic effect.</div></div><div><h3>Results</h3><div>Our bioinformatics identified a nine-gene oncogenic network in HNSCC enriched in inflammatory and profibrotic pathways. A core three-gene SIS oncogenic signature (SERPINE1, INHBA, SPP1) was identified. High SIS expression correlated with poor survival and increased CAF infiltration. Docking predicted favorable binding of ovatodiolide to SERPINE1, SPP1, and INHBA. CAF-conditioned medium enhanced the stemness and chemoresistance of HNSCC cells, promoting SIS signature and stemness markers. Ovatodiolide suppressed oncogenic properties and CAF activation, decreasing SIS and CAF markers. In a mouse model, ovatodiolide overcame cisplatin resistance by reducing the SIS signature.</div></div><div><h3>Conclusion</h3><div>The SIS signature contributes to HNSCC progression, stemness, and drug resistance by facilitating CAF generation. Ovatodiolide disrupts this signature and inhibits CAF transformation.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 1","pages":"Pages 282-293"},"PeriodicalIF":3.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145904039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}