Journal of Complementary and Integrative Medicine最新文献

{"title":"Chenopodium ambrosioides Linn. induces mitochondrial-dependent cell death via activation of mPT pore opening, caspases and apoptotic machinery.","authors":"Adeola Oluwakemi Olowofolahan, Mimidoo Gaaba, Fatima Bulus Musa","doi":"10.1515/jcim-2025-0312","DOIUrl":"https://doi.org/10.1515/jcim-2025-0312","url":null,"abstract":"<p><strong>Objectives: </strong>Mitochondrion is an organelle involved in cellular energy generation and cell death. The mitochondrial permeability transition (mPT) pore plays a crucial role in the execution of mitochondrial-dependent cell death. Several medicinal plants evoke their therapeutic potential against tumor and cancer by instigating mitochondrial-dependent cell death. This research consequently explored the effect of chloroform fraction of <i>Chenopodium ambrosioides</i> (CFCA) on mitochondrial-dependent cell death via mPT pore.</p><p><strong>Methodology: </strong>Rats were equally assigned into four groups; A (control), B (50), C (100) and D (200) mg/kg CFCA. Mitochondria were isolated by differential centrifugation. The mPT pore opening, mitochondrial ATPase (mATPase) activity and mitochondrial-lipid peroxidation (mLPO) were assessed spectrophotometrically. Caspases 9 and 3 levels, hepatic DNA fragmentation, histology and immunohistochemical analysis of Bax, Cytochrome <i>c</i>, P53 and anti-apoptotic Bcl-2 proteins were determined. Gas Chromatography-Mass Spectrometry (GC-MS) analysis of CFCA was carried out.</p><p><strong>Results: </strong>The CFCA dose-dependently induced mPT pore opening, enhanced mATPase activity, reduced malondialdehyde generation, increased Caspases 9 and 3 levels and effected hepatic DNA fragmentation. The histological assessment showed mild to moderate steatosis and infiltration of the hepatocytes. The CFCA upregulated the expression of Bax, Cytochrome <i>c</i> and P53 proteins while anti-apoptotic Bcl-2 protein was downregulated. The GC-MS analysis revealed the presence of certain pharmacologically reactive compounds including Tridecanoic acid, 12-methyl ester, Hexadecanoic acid methyl ester, 11-Octadecadienoic acid, methyl ester, Pyrido[2,3-d]pyrimidine and 6-Undecyl-5,6-dihydro-2H-pyran-2-one.</p><p><strong>Conclusions: </strong>This study suggests the presence of phytochemicals in CFCA that could execute mitochondrial-mediated apoptosis via induction of mPT pore opening, caspases and apoptotic machinery.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147674068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antidepressant-like effect of <i>Peperomia pellucida</i> (L.) Kunth extract: analysis of behavioral and oxidative stress parameters in mice exposed to chronic unpredictable mild stress.","authors":"Fitrya Fitrya, Ghina Desfitriyana, Nyiayu Fauziyah Kurniawati, Vitri Agustiarini, Annisa Amriani, Rennie Puspa Novita","doi":"10.1515/jcim-2025-0391","DOIUrl":"https://doi.org/10.1515/jcim-2025-0391","url":null,"abstract":"<p><strong>Objectives: </strong><i>Peperomia pellucida</i> (Indonesian name: Suruhan) is a tropical plant easily found throughout Indonesia. This plant is used as a food ingredient and to treat various diseases due to its phytoconstituent content. This study aimed to evaluate the antidepressant effect of the ethanol extract of the Suruhan herb.</p><p><strong>Methods: </strong>Mice were exposed to chronic mild stress using the chronic unpredictable mild stress (CUMS) method for 21 days, followed by a sucrose preference test. The antidepressant effect was assessed by behavioral changes in the tail suspension test (TST), forced swimming test <b></b> (FST), and locomotor tests. Oxidative stress levels were measured using malondialdehyde (MDA) and catalase levels, and the evaluation was complemented by histopathological analysis. The antidepressant effect of the Suruhan extract (ES) was compared with that of normal controls, stress controls, and fluoxetine controls.</p><p><strong>Results: </strong>In the behavioral test, there was no significant difference in immobility time and number of line crossings in the test group compared to the fluoxetine control (p>0.05). ES significantly increased the sucrose preference value of the test group. Oxidative stress parameter analysis showed that the extract reduced MDA levels in mice, no different from the fluoxetine control group (p>0.05), and increased catalase levels, but not as effectively as the standard drug (p<0.05). Histopathological analysis found a significant increase in the thickness of the granular layer in the dentate gyrus of mice treated with ES and fluoxetine compared to the stress group.</p><p><strong>Conclusions: </strong>Suruhan herb has antidepressant-like effects and has the potential to be used as a functional food and complementary medicine in the treatment of depression.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147654379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Black Maitake mushroom (<i>Grifola frondosa</i>) extract exerts anti-cancer effects and enhances the efficacy of chemotherapeutic drugs in 2D and 3D models of triple-negative breast cancer.","authors":"Silvia DeMarco, Sona Regonda, Siyaram Pandey","doi":"10.1515/jcim-2025-0255","DOIUrl":"10.1515/jcim-2025-0255","url":null,"abstract":"<p><strong>Intoduction: </strong>Triple-negative breast cancer (TNBC) is an aggressive breast cancer subtype that lacks hormone receptors and HER2 amplification, limiting targeted therapy options. Chemotherapeutics like cisplatin and taxol are commonly used but lack selectivity, often causing harsh side effects. These limitations have led to increased interest in natural health products (NHPs) as complementary treatments due to their potential anticancer benefits and lower toxicity. Black Maitake mushroom (<i>Grifola frondosa</i>), used in traditional Chinese medicine, contains polysaccharides with reported anticancer activity. However, further research is needed on the whole extract, its mechanism, and its interaction with chemotherapy.</p><p><strong>Objectives: </strong>This study assessed the anticancer effects of Black Maitake Odaira Extract - Prothera (BMOE; manufactured by Shogun Maitake Canada, London, ON) on the MDA-MB-231 TNBC cell line. BMOE was evaluated alone and in combination with chemotherapeutic agents cisplatin and taxol using both 2D and 3D <i>in vitro</i> models.</p><p><strong>Methods: </strong>Apoptosis was evaluated using Hoechst 33342, Annexin V, and propidium iodide staining. Mitochondrial membrane depolarization was assessed via TMRM. A wound-healing assay measured cell migration. Structural effects were examined in 3D spheroid cultures following treatment.</p><p><strong>Results: </strong>BMOE induced dose- and time-dependent apoptosis and mitochondrial depolarization. In 3D models, BMOE disrupted spheroid structure. The wound-healing assay showed impaired TNBC cell migration. Most importantly, BMOE significantly enhanced chemotherapeutic efficacy when combined with cisplatin or taxol.</p><p><strong>Conclusions: </strong>These findings highlight the potential of BMOE as a non-toxic adjunct that enhances the efficacy of standard chemotherapies to contribute to the development of safer, more targeted treatment strategies for TNBC.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":"23 2","pages":"296-307"},"PeriodicalIF":0.0,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148455497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydroethanolic root extract of <i>Ficus capensis</i> exhibits antiulcerogenic activities in acetic acid-induced colitis rat model.","authors":"George Owusu, Meshack Antwi-Adjei, Yeboaba Kofi Opoku-Yeboah, Barnabas Tuatil, Abigail Adu-Boateng, Frederick Emmanuel Baidoo, Francis Kwame Abrokwah, Benjamin Aboagye","doi":"10.1515/jcim-2025-0389","DOIUrl":"https://doi.org/10.1515/jcim-2025-0389","url":null,"abstract":"<p><strong>Objectives: </strong><i>Ficus capensis</i> (FCE) is widely used in Ghana and some other West African countries to treat gastrointestinal diseases. This study examines the antiulcerogenic properties of the ethanol-based FCE root extract in rats using acetic acid-induced ulcerative colitis model.</p><p><strong>Methods: </strong>Rats were arbitrarily assigned to 6 groups (n=5) and given either oral normal saline (10 mL/kg), sulfasalazine (500 mg/kg), or FCE (30, 100, and 300 mg/kg) for 7 days. To induce colitis, 500 μL of acetic acid (4 % v/v) was given intrarectally on day 2. On the 8th day, rats were euthanized. Hematology, antioxidant, and histopathological investigations were assessed.</p><p><strong>Results: </strong>FCE significantly prevented body weight loss and reduced the colon weight/length ratio to 0.106 ± 0.004 g/cm relative to the control. Additionally, FCE reduced DAI to 2.035 ± 0.1216 compared to the control. The histopathology showed that FCE-treated colons reduced inflammatory scores compared to the control. Levels of NF-κB and TNF-α, gross colonic damage and epithelial cell loss were reduced. FCE elevated colonic SOD and CAT activity to 12.60 ± 1.327 and 9.60 ± 0.6713, correspondingly. Furthermore, the amount of MDA decreased to 29.72 ± 1.974 compared to the control. FCE increased levels of RBC and HGB, respectively, to 8.64 ± 0.48 (×10⁶/µL) and 9.36 ± 0.62 g/dL and lowered WBC and NEU to 10.27 ± 0.36, 9.35 ± 0.62, 20.43 ± 0.82, and 18.47 ± 0.47, respectively, compared to the control.</p><p><strong>Conclusions: </strong>Hydroethanolic root extract of FCE possesses antiulcerogenic effects.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147645309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction of: Evaluation of the protective effect of the <i>Echinacea Purpurea</i> against apoptosis and inflammation induced by overdose of iron dextran on the hematopoietic system.","authors":"Doaa Kahim Abdul Ridha, Ali Faris Hassan","doi":"10.1515/jcim-2026-2001","DOIUrl":"10.1515/jcim-2026-2001","url":null,"abstract":"","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":"23 1","pages":"200"},"PeriodicalIF":0.0,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147874272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Successful integration of Yoga and Naturopathy with conventional medicine for health promotion: insights from the Tamil Nadu model in rural India.","authors":"Vaishnu Karthik Kamaraj, Srikaren Udhayakumar, Yeswanth Kumaran Venkatachalam, Poornima Ravi, Maheshkumar Kuppusamy","doi":"10.1515/jcim-2025-0419","DOIUrl":"10.1515/jcim-2025-0419","url":null,"abstract":"","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":"357-359"},"PeriodicalIF":0.0,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147627851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromatographic analysis of <i>Crataegus azarolus</i> and possible psychiatric implications.","authors":"Bekir Nihat Dogrul, Ibrahim Kiliccalan, Ekrem Samet Asci","doi":"10.1515/jcim-2025-0397","DOIUrl":"https://doi.org/10.1515/jcim-2025-0397","url":null,"abstract":"<p><strong>Background: </strong><i>Crataegus azarolus</i> (hawthorn) is a medicinal plant traditionally used for various health benefits and culinary reasons, although its psychiatric effects remain poorly studied. This study aimed to assess the volatile chemical composition and ionic profile of <i>C. azarolus</i>, and to integrate these findings with a targeted review of the literature to propose potential psychiatric implications.</p><p><strong>Methods: </strong>Methanolic extracts of dried flowers, leaves, and berries were analyzed by gas chromatography-mass spectrometry (GC-MS), with compounds identified using reference libraries. Ion chromatography (IC) with anion and cation columns was used to quantify inorganic ions, expressed as mg per 100 g dried material.</p><p><strong>Results: </strong>GC-MS tentatively identified 13 major compounds, including flavonoids, fatty acids, sterols, vitamin D derivatives, and digitoxin. Many of these constituents have been suggested having anti-inflammatory, antioxidant, anxiolytic, and antidepressant-like activities in preclinical studies. Among the compounds, the flavonoids quercetin and lucenin-2 were particularly notable due to their proposed roles in central nervous system function, neuroprotection and possible psychiatric effects. IC analysis additionally revealed the presence of various inorganic ions, although their overall concentrations were low.</p><p><strong>Conclusions: </strong>The biochemical complexity of <i>C. azarolus</i> highlights its potential relevance for mental health, yet direct preclinical and clinical evidence remains lacking. Future investigations are needed to clarify its psychiatric applications.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147486149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroprotective role of <i>Bougainvillea spectabilis</i> in ketamine-induced neurotoxicity: targeting oxidative stress and neuroinflammation.","authors":"Rania Elgohary, Omar M E Abdel-Salam, Eman R Youness, Enayat A Omara, Amany A Sleem","doi":"10.1515/jcim-2025-0175","DOIUrl":"10.1515/jcim-2025-0175","url":null,"abstract":"<p><strong>Objectives: </strong>Schizophrenia is a persistent and devastating mental illness that affects around 1 % of the population. Despite the availability of antipsychotic drugs, their limited efficacy and significant adverse effect profiles have sparked an increased interest in finding well-tolerated, safer alternatives. Recently the bioactive compounds that are produced from natural sources as possible supplementary therapy to reduce the symptoms of schizophrenia. This study seeks to evaluate the potential therapeutic effects of <i>Bougainvillea spectabilis (B. spectabilis)</i> in alleviating oxidative stress and neuroinflammatory pathways associated with ketamine-induced biochemical and histopathological changes.</p><p><strong>Methods: </strong>Rats were administered ketamine (30 mg/kg, s.c.) to cause neurotoxicity. <i>B. spectabilis</i> extract at dosages of 50 and 100 mg/kg, were administrated 30 min later.</p><p><strong>Results: </strong><i>B. spectabilis</i> extract significantly decreased oxidative stress, including homocysteine and malondialdehyde (MDA), while increasing endogenous antioxidant defenses such as reduced glutathione (GSH) and folic acid levels. The extract also attenuated neuroinflammatory responses through downregulation of TNF-α and NF-κB expression and markedly improved the histopathological architecture of brain tissue.</p><p><strong>Conclusions: </strong>The study demonstrates that <i>B. spectabilis</i> extract exerts neuroprotective effects through antioxidant and anti-inflammatory mechanisms. These findings suggest that the extract may represent a promising adjunct therapeutic strategy for mitigating neurotoxicity associated with oxidative stress and neuroinflammation.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":"308-317"},"PeriodicalIF":0.0,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147486140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum TNF-α, oxidized LDL, and APOCII as novel predictors for familial mediterranean fever in Egyptian children: a cross-sectional study.","authors":"Hala T El-Bassyouni, Khaled Hamed, Eman Refaat Youness, Hisham A Orban, Abeer Selim, Ahmed Adel, Rehab S I Moustafa, Randa Salah Lotfy, Maha Mahmoud A Kobesiy, Mina Wassef Girgiss, Sherif H Elwan, Hanan Farouk, Ramy Mohamed, Hanaa Reyad Abdallah, Moushira Zaki","doi":"10.1515/jcim-2025-0388","DOIUrl":"10.1515/jcim-2025-0388","url":null,"abstract":"<p><strong>Intoduction: </strong>Persistent subclinical inflammation is a hallmark of familial Mediterranean fever (FMF), the most common autoinflammatory disease. Among the key mediators implicated in this chronic inflammatory state are tumor necrosis factor-alpha (TNF-α), oxidized low-density lipoprotein (OxLDL), and apolipoprotein C-II (APOC2), which contribute to the dysregulated immune response characteristic of FMF. The present study aims to evaluate the potential role of TNF-α, OxLDL, and APOC2 as diagnostic biomarkers of disease severity in patients with FMF.</p><p><strong>Methods: </strong>The study enrolled 66 patients diagnosed with FMF and 60 age- and gender-matched healthy controls. Serum levels of tumor necrosis factor-alpha (TNF-α) and oxidized low-density lipoprotein (OxLDL) were quantified using the enzyme-linked immunosorbent assay (ELISA) technique. However, the apolipoprotein C-II (APOC2) concentrations were measured using an automated biochemistry analyzer. Furthermore, the Carotid intima media was assessed.</p><p><strong>Results: </strong>Serum TNF-α levels were significantly higher in FMF patients than in the control group, emphasizing the protein's role as an important marker of chronic inflammation. Levels of low-density lipoprotein (OxLDL) and apolipoprotein C-II (APOC2) were also notably altered in the FMF cohort. Notably, OxLDL, APOC2 and TNF-α demonstrated strong positive correlation with attack frequency, multiple linear regressions analysis showed that OxLDL (β=1.53, p=0.002) and TNF-α (β=1.38, p=0.003) were significant predictors for higher number of attacks, suggesting their potential role involvement in the inflammatory cascade and their utility as complementary biomarkers in FMF. The carotid intima-media thickness (CIMT) of patients and control subjects did not differ statistically significantly.</p><p><strong>Conclusions: </strong>TNF-α, oxidized LDL, and APOC2 are key pro-inflammatory mediators that may serve as novel biomarkers for assessing chronic inflammatory status in FMF patients. Their elevated levels could provide valuable insights into disease progression and help guide personalized treatment strategies.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":"23 2","pages":"349-356"},"PeriodicalIF":0.0,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148455495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The <i>Journal of Complementary and Integrative Medicine</i> is switching its publication model to open access.","authors":"Suzanne Mekking, Jeremy Y Ng","doi":"10.1515/jcim-2026-0056","DOIUrl":"10.1515/jcim-2026-0056","url":null,"abstract":"","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147473884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}