Journal of Complementary and Integrative Medicine最新文献

{"title":"Prevalence of chemotherapy-induced anemia and its impact on quality of life in breast cancer patients.","authors":"Ramsha Batool, Tehreem Hayat, Hira Rizwan Butt, Fatima Um Ul Baneen, Hooria Fayyaz, Tayyaba Rafiq, Mehrulnisa Akhtar, Uswa Fayyaz, Maria Ahmed, Ayesha Azeem, Mah Gul Khan, Mahnoor Ikram Khan, Ali Farooq, Amina Shahid","doi":"10.1515/jcim-2026-0231","DOIUrl":"https://doi.org/10.1515/jcim-2026-0231","url":null,"abstract":"<p><strong>Background: </strong>Chemotherapy-induced anemia (CIA) is a frequent condition among breast cancer patients who are treated with systemic chemotherapy, and is linked to fatigue, decreased functional capacity, and poor quality of life.</p><p><strong>Objectives: </strong>To determine the prevalence of chemotherapy-induced anaemia and evaluate its impact on the quality of life of breast cancer patients receiving chemotherapy.</p><p><strong>Methods: </strong>This cross-sectional study was performed at the Ghurki Trust Teaching Hospital in Lahore, Pakistan, from January 2024 to April 2025. The trial included 100 female patients with histopathologically confirmed breast cancer who had received a minimum of three treatment cycles. Anaemia was determined using World Health Organization guidelines (Hb<12 g/dL), and haemoglobin was assessed using an automated haematology analyser. The quality of life was evaluated via the EORTC QLQ-C30 questionnaire. The data were examined with SPSS version 27.0.</p><p><strong>Results: </strong>The average age of participants was 50.7 ± 10.8 years. Anaemia produced by chemotherapy was seen in 61.0 % of patients, including mild (30.0 %), moderate (24.0 %), and severe (7.0 %) cases. Anaemic patients had a much lower global health status score than non-anaemic patients (54.3 ± 12.7 vs. 74.8 ± 10.4, p<0.001). There were significant reductions in physical, emotional, and social functioning ratings, with an elevation in tiredness scores (65.9 ± 16.1 vs. 37.8 ± 11.5, p<0.001). Moderate-to-severe anaemia independently forecasted decreased quality of life (OR=3.48, 95 % CI: 1.62-7.45, p=0.001).</p><p><strong>Conclusions: </strong>Patients with breast cancer undergoing chemotherapy often suffer from anaemia, which negatively impacts their quality of life. Appropriate management may improve treatment outcomes early on.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148865021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of anti-osteoarthritic activity of hydroalcoholic extract of <i>Moringa oleifera</i> by using papain-inducing model in experimental animals.","authors":"Vaibhav Sangameshwar Gavane, Snehal Ambadas Gojare, Deepti Dinesh Bandawane","doi":"10.1515/jcim-2026-0236","DOIUrl":"https://doi.org/10.1515/jcim-2026-0236","url":null,"abstract":"<p><strong>Background: </strong>The anti-inflammatory, anti-arthritic, and antioxidant qualities of <i>Moringa oleifera</i> have long been used to treat inflammation and various types of arthritis.</p><p><strong>Objectives: </strong>To evaluate the hydroalcoholic extract of <i>Moringa oleifera</i> pods in a papain-induced animal model.</p><p><strong>Materials and methods: </strong>Hydroalcoholic extraction was used to obtain the hydroalcoholic extract of <i>Moringa oleifera</i> (HEMO), and its phytochemical constituents were examined. In Wistar rats, osteoarthritis was induced by intra-articular injection using a Papain-induced model, and Glucosamine sulfate was used as a conventional standard treatment. Experimental animals were given HEMO pods at 100 mg/kg and 200 mg/kg orally. Knee joint diameter, joint stiffness score, locomotor activity, body weight, and post-sacrifice Biochemicals like Matrix Metalloproteins-13, C-Reactive Proteins, Alkaline Phosphatase, Sodium Dismutase, Catalase (MMP-13, CRP, ALP, SOD, CAT), Haematological - Erythrocyte Sedimentation Rate (ESR), Cytokines like Interleukin-1β and Tumour Necrosis Factor-α (IL-1β, TNF-α), radiological, and histopathological markers were done to assess anti-osteoarthritic activity of extract.</p><p><strong>Results: </strong>Hydroalcoholic extract of <i>Moringa oleifera</i> (HEMO) at 200 mg/kg significantly reduced knee diameter and joint stiffness in papain-induced arthritic rats by upregulating antioxidant agents and downregulating inflammatory mediators. In addition, the chondroprotective activity of the HEMO 200 mg/kg extract against OA was validated by histological and radiographic (X-ray) assessments, thereby scientifically confirming the traditional use of HEMO in the management of inflammation and osteoarthritis. The hypothesis that HEMO can be used to treat arthritis is adequately supported based on these standards of evidence.</p><p><strong>Conclusions: </strong>In the research, HEMO at a dosage of 200 mg/kg revealed a significant anti-arthritic effect, confirming its conventional usage. The results offer sufficient preclinical validation for potential therapeutic use. These results indicate that HEMO may potentially be used as a natural treatment for managing inflammation and arthritis.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148829139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidative stress-driven metabolic interactions underlying the hepatoprotective effects of a phytocomplex in ethanol-induced liver injury.","authors":"Rana Jafarova, Sevda Guliyeva, Sevinj Abasova, Nigar Guliyeva, Narmina Jabbarova, Anaxanim Yusifova, Besti Asadova","doi":"10.1515/jcim-2026-0117","DOIUrl":"https://doi.org/10.1515/jcim-2026-0117","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to investigate the interactions between biochemical, oxidative, and morphological markers of liver injury in an experimental model of alcoholic hepatitis and to evaluate the hepatoprotective efficacy of a herbal phytocomplex (AZHEPOFIT) within an integrated mechanistic framework.</p><p><strong>Methods: </strong>Alcoholic hepatitis was induced in 40 male rats by oral ethanol administration combined with a high-fat diet for 21 days. From day 22, animals received AZHEPOFIT (1 mL/100 g body weight) daily for 14 days. Biochemical parameters, oxidative stress markers, antioxidant status, lipid profile, and liver histomorphology were assessed. Correlation analysis was performed using Spearman's coefficient.</p><p><strong>Results: </strong>Alcoholic hepatitis was associated with pronounced cytolysis, cholestasis, oxidative stress, dyslipidemia, and marked histopathological alterations, including steatosis, hepatocyte ballooning, inflammation, necrosis, and early fibrosis. AZHEPOFIT administration significantly reduced cytolytic enzyme activity and lipid peroxidation, enhanced antioxidant defense, improved lipid metabolism, and attenuated structural liver damage. Strong correlations exist between oxidative stress markers and cytolytic enzymes, and inverse relationships with antioxidant parameters, indicating a coordinated pathogenic network linking oxidative stress, lipid metabolism, and hepatocellular injury.</p><p><strong>Conclusions: </strong>The hepatoprotective effects of AZHEPOFIT are associated with the modulation of interconnected oxidative stress-metabolic pathways. These findings provide mechanistic insight into phytotherapeutic intervention in alcohol-induced liver injury and support targeting redox-dependent pathways as a promising therapeutic strategy.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148808651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Behavioral and neurochemical assessment of intranasal gel containing <i>Cocculus hirsutus</i> extract in experimental anxiolytic Models.","authors":"Pallavi Jadhav, Suvarna Ingale, Prabha Bhong","doi":"10.1515/jcim-2026-0159","DOIUrl":"https://doi.org/10.1515/jcim-2026-0159","url":null,"abstract":"<p><strong>Objectives: </strong>The present study was designed to develop and evaluate an intranasal gel formulation of <i>Cocculus hirsutus</i> extract (HACHL) for the management of anxiety. The primary objective was to enhance brain delivery through the intranasal route by prolonging the residence time of the formulation.</p><p><strong>Methods: </strong>The hydroalcoholic extract of <i>Cocculus hirsutus</i> leaves (HACHL) was subjected to preliminary phytochemical screening. Its anxiolytic activity was evaluated using various animal models, including the elevated plus maze, open field test, and light-dark box test. A intranasal gel formulation was developed using suitable polymers and excipients to facilitate intranasal delivery. The formulated gel was evaluated for physicochemical parameters. The anxiolytic activity of the developed intanasal gel was further assessed and compared with the plain HACHL and standard drug.</p><p><strong>Results: </strong>The HACHL exhibited significant anxiolytic activity, as evidenced by increased time spent in open arms, increased crossing, and reduced anxiety-related behaviour in experimental models. The developed intranasal gel showed acceptable physicochemical properties and a sustained drug release profile. Pharmacological evaluation of the intranasal gel demonstrated enhanced anxiolytic activity compared to the HACHL alone, indicating improved drug delivery and efficacy.</p><p><strong>Conclusions: </strong>The findings suggest that HACHL possesses significant anxiolytic potential. The intranasal gel enhances its therapeutic effectiveness, due to potential to enhance nose to brain delivery and bioavailability. This study supports the potential application of intranasal delivery systems as a promising approach for the management of anxiety disorders.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148793990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of non-alcoholic fatty liver disease with a special focus on evidence-based Unani therapeutic approaches: a scoping review.","authors":"Nida Mehraj, Atiba Jan, Pawan Kumar, Rameesa Rashid","doi":"10.1515/jcim-2026-0065","DOIUrl":"https://doi.org/10.1515/jcim-2026-0065","url":null,"abstract":"<p><p>Non-alcoholic fatty liver disease (NAFLD) is a chronic metabolic liver disorder affecting nearly one-quarter of the global population. It is closely associated with obesity, type 2 diabetes mellitus, dyslipidaemia, and other components of metabolic syndrome, and may progress to non-alcoholic steatohepatitis, fibrosis, cirrhosis, and hepatocellular carcinoma. Despite its increasing prevalence, effective pharmacological options remain limited, and sustained lifestyle modification continues to be the cornerstone of management. In Unani medicine, conditions resembling NAFLD are described under <i>Sū'-i-Mizāj Kabid Bārid</i> and <i>Waram al-Kabid Balghamī.</i> A scoping review methodology was employed to map the breadth of available evidence on NAFLD from both conventional and Unani medical perspectives. Electronic databases PubMed, ScienceDirect, DOAJ, Google Scholar, and the AYUSH Research Portal were searched using predefined Boolean search strings. Classical Unani texts were examined manually. Unani literature describes therapeutic approaches centred on '<i>Ilāj bi'l-Ghidhā</i> (diet therapy), <i>'Ilāj bi'l-Tadbīr</i> (regimenal therapy), and <i>'Ilāj bi'l-Dawā</i> (pharmacotherapy). Several single and compound formulations possessing hepatoprotective, antioxidant, hypolipidaemic, anti-inflammatory, and hypoglycaemic properties have shown potential benefits in preclinical and clinical studies. Available evidence suggests that Unani interventions may offer therapeutic benefit in NAFLD; however, well-designed clinical trials are required to establish their safety and efficacy.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148794007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis of gold nanoparticles from <i>Berberis Lycium</i> and their impact on liver and kidney health in albino mice.","authors":"Almas Azhar, Maria Abdullah Butt, Madiha Aziz, Muhammad Kamran Latif, Razia Batool, Umme Ammara, Insha Zanib, Manahil Kanwal, Maryam Naz, Shazia Khushid, Arslan Shuja","doi":"10.1515/jcim-2026-0122","DOIUrl":"https://doi.org/10.1515/jcim-2026-0122","url":null,"abstract":"<p><strong>Objectives: </strong>Gold nanoparticles (AuNPs) possess unique physicochemical and biomedical properties; however, conventional synthesis approaches often involve hazardous chemicals that may limit biocompatibility. This study aimed to synthesize AuNPs using aqueous extract of <i>Berberis lycium</i>, characterize their physicochemical properties, and evaluate their toxicological effects in albino mice.</p><p><strong>Methods: </strong>AuNPs were synthesized by incubating aqueous leaf extract of <i>B. lycium</i> with HAuCl₄ (1 mM) in a 1:9 ratio. Characterization was performed using UV-Visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). 40 albino mice were divided into four groups (n=10): control, 10 mg/kg, 25 mg/kg, and 50 mg/kg AuNPs administered intraperitoneally for 14 days. Liver and kidney tissues were examined through gross and histopathological analyses.</p><p><strong>Results: </strong>UV-Visible spectroscopy confirmed AuNP formation with an absorption peak at 535 nm. XRD analysis demonstrated a face-centered cubic crystalline structure, while FTIR revealed phytochemical-mediated reduction and stabilization of nanoparticles. SEM imaging showed well-dispersed nanoparticles with predominantly cubic to hexagonal morphology and particle sizes ranging from 10-30 nm. Toxicological evaluation demonstrated no significant histopathological alterations at 10 mg/kg. Mild hepatic steatosis and renal inflammatory infiltration were observed at 25 mg/kg, whereas severe hepatotoxicity and nephrotoxicity, including necrosis, hemorrhage, glomerular distortion, and tubular degeneration, were evident at 50 mg/kg.</p><p><strong>Conclusions: </strong>Green-synthesized AuNPs derived from <i>B. lycium</i> are stable and relatively biocompatible at lower concentrations but produce significant dose-dependent hepatic and renal toxicity at higher doses. These findings highlight the importance of careful dose optimization and comprehensive toxicological assessment before biomedical application of plant-mediated gold nanoparticles.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148760659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of beetroot (<i>Beta vulgaris</i> L.) ethanolic extract in the nephrolithiasis rat model.","authors":"Fitri Rachmaini, Dwisari Dillasamola Isberd, Winda Adzka Fitri, Rahmad Abdillah","doi":"10.1515/jcim-2025-0451","DOIUrl":"https://doi.org/10.1515/jcim-2025-0451","url":null,"abstract":"<p><strong>Objectives: </strong>Nephrolithiasis is a prevalent urinary condition that often leads to the development of secondary stones. The biological activity of red beetroot (<i>Beta vulgaris</i> rubra) and its potential as a disease-prevention and health-promoting agent have garnered considerable attention in recent years. Our objective was to investigate the impact of <i>B. vulgaris</i> Ethanolic Extract (EEBV) in the nephrolithiasis rat model.</p><p><strong>Methods: </strong>Thirty Wistar Kyoto rats were used and randomly divided into five groups. Except for the normal control group, 1 % ethylene glycol (EG) and 1 % ammonium chloride (AC) were added to drinking water for 14 days to induce nephrolithiasis. The intervention was administered to all animal groups except the normal controls. All animals were: a normal control group(Na CMC), a nephrolithiasis group (EG 1 %), the EEBV 125 group (nephrolithiasis rats given EEBV 125 mg/Kg BW per oral), the EEBV 250 group (nephrolithiasis rats given EEBV 250 mg/Kg BW), and the EEBV 500 group (nephrolithiasis rats given EEBV 500 mg/Kg BW). Biochemical assessments were performed on blood and urine samples. Crystal deposition and renal tissue damage were assessed using haematoxylin and eosin staining.</p><p><strong>Results: </strong>EEBV administration significantly improved serum and urine parameters. Histopathological analysis revealed that EEBV administration decreased renal alteration and crystal deposition. EEBV 125 and EEBV 250 have the greatest effect on reducing crystal deposition and improving renal function.</p><p><strong>Conclusions: </strong>The study demonstrates that EEBV has beneficial effects in reducing the incidence of kidney stones and improving kidney function.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148447440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of combined nutritional supplementation with biotin, omega-3 polyunsaturated fatty acids and vitamin D in depression: a preclinical study.","authors":"Harsha Gautam, Amrita Parida, Shreya Hegde, Siddhanth Rangineni, Vijetha Shenoy Belle, Mohandas Rao Kappettu Gadahad, Dhanya R Rai, Juwairiya Sayyad","doi":"10.1515/jcim-2026-0080","DOIUrl":"https://doi.org/10.1515/jcim-2026-0080","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the antidepressant-like effects of combined nutritional supplementation with omega-3 fatty acids, vitamin D, and biotin, alone and in combination with half-dose fluoxetine, in a Chronic Unpredictable Mild Stress (CUMS) model of depression in rats.</p><p><strong>Methods: </strong>Thirty Sprague-Dawley rats were divided into five groups: control, disease control, fluoxetine-treated (5 mg/kg), nutrient combination (omega-3 fatty acids, vitamin D, and biotin), and nutrient combination with half-dose fluoxetine (2.5 mg/kg). Depression was induced using the CUMS model for four weeks. Behavioral assessments were performed using the sucrose preference test and elevated plus maze (EPM). Biochemical parameters including malondialdehyde (MDA), superoxide dismutase (SOD), catalase, thiol levels, and brain-derived neurotrophic factor (BDNF) were estimated. Histopathological examination of hippocampus and dentate gyrus was conducted. Data were analyzed using one-way analysis of variance followed by Tukey's post hoc test.</p><p><strong>Results: </strong>The nutrient combination and half-dose fluoxetine with nutrient combination groups showed significant behavioral improvement vs. the disease control group, with higher sucrose preference and greater open-arm time in the EPM. Catalase activity was significantly higher in the nutrient combination group (2.50 ± 0.71 vs. 0.16 ± 0.03 pmol/min), while the half-dose fluoxetine with nutrient combination group demonstrated significantly higher SOD (2.11 ± 0.03 vs. 0.07 ± 0.01 milliunit/mg protein) and lower MDA (1.35 ± 0.10 vs. 4.70 ± 0.09 μM/L) compared with disease control. Both these groups showed increased healthy neurons in the hippocampus and dentate gyrus in histopathological analysis, suggesting neuroprotection and neuronal regeneration.</p><p><strong>Conclusions: </strong>Combined nutrient supplementation demonstrated significant antidepressant-like effects compared to fluoxetine alone, indicating potential adjunctive benefits requiring further validation.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148360820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peruvoside, a cardiac glycoside, induces cell cycle arrest in non-small cell lung cancer (A549 cell line) and modulates PI3K/AKT/mTOR.","authors":"Zahraa Shehab Ahmed, Meroj Ahmed Jasem, Bahir Abdul-Razzaq Mshimesh, Safaa A Al-Qaysi","doi":"10.1515/jcim-2026-0096","DOIUrl":"10.1515/jcim-2026-0096","url":null,"abstract":"<p><strong>Objectives: </strong>Lung cancer constitutes a global health crisis that leads to mortality. The cellular signaling and cell cycle are disrupted in lung cancer. From time immemorial, plants have been a major source of medications. Notably, cardiac glycosides (CGs) potently bind to Na⁺/K⁺-ATPase in cardiac tissue. CGs show promising potential as anticancer activity. Peruvoside belongs to the cardiac glycosides, Peruvoside derived from <i>Thevetia peruviana</i>. Noteworthy, Peruvoside has shown promising interest due to its powerful effect toward several cancer types. The presented study evaluates Peruvoside's effects on the proliferation as well as cell cycle progression of lung cancer A549 cells to validate its anticancer potential.</p><p><strong>Methods: </strong>A549 cells were cultured and allocated into two groups: treated with Peruvoside and an untreated control group. Viability of A549 cells was assessed by WST-8 assay; cell cycle analysis was performed by flow cytometry; CDK6 and cyclin D1 protein expression was determined by Western blot; and real-time PCR analysis was performed to identify the expression of <i>CHK1, CHK2, PI3K, AKT,</i> and <i>mTOR</i> genes.</p><p><strong>Results: </strong>Peruvoside at a concentration of 68.77 nM was associated with a notable anti-proliferative effect and the induction of an apoptotic cascade. Peruvoside induces cell cycle arrest at the G2/M phase, validated by Western blot and real-time PCR analysis, confirming that inhibition of proteins and genes related to cell cycle progression and expansion of the lung cancer, indicating that Peruvoside has antiproliferative and pro-apoptotic effects.</p><p><strong>Conclusions: </strong>Within this research, the data showed the arrest of the cell cycle and the inhibition of the proliferation regulatory gene by Peruvoside were associated with suppression of lung cancer survival, proliferation, metastatic dissemination, and promotion of cell death.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148345521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the anticancer activity of black maitake odaira extract-prothera (<i>Grifola frondosa</i>) and its interactions with common chemotherapeutics in <i>in-vitro</i> and <i>in-vivo</i> models of human colorectal cancers.","authors":"Hannah Drew, Vanessa Chimienti, Sona Regonda, Mohammad El Hindawi, Divyanshi Mitra, Eesha Atikukke, Angelina Hermes, Siyaram Pandey","doi":"10.1515/jcim-2025-0300","DOIUrl":"https://doi.org/10.1515/jcim-2025-0300","url":null,"abstract":"<p><strong>Objectives: </strong>Black maitake odaira extract-prothera (<i>Grifola frondosa</i>; BME), a β-glucan-rich natural product, has demonstrated anticancer activities including immunomodulation, inhibition of metastasis, enhanced chemotherapy sensitivity, and induction of apoptosis. This study investigated the effects of BME on human colorectal cancer cell lines HCT-116 and HT-29, evaluated its interaction with FOLFOX chemotherapy, and assessed selectivity using the healthy human colon epithelial cell line NCM-460.</p><p><strong>Methods: </strong>Anticancer activity was evaluated in two- and three-dimensional colorectal cancer cell models by measuring apoptosis and mechanism of action using fluorogenic markers, cell viability via formazan reduction, and migration through wound-healing assays. Selectivity was assessed in healthy NCM-460 colon epithelial cells. <i>In vivo</i> antitumour efficacy was examined using xenografted immunocompromised mouse models.</p><p><strong>Results: </strong>BME induced time- and dose-dependent apoptosis, reduced viability and migration, increased reactive oxygen species, and mediated mitochondrial dysfunction. Positive interactions were observed with FOLFOX chemotherapy. <i>In vivo</i>, BME enhanced antitumour activity, while no apoptotic effects were detected in normal NCM-460 colon epithelial cells, indicating selectivity.</p><p><strong>Conclusions: </strong>These findings suggest BME may have potential as an adjunct to colorectal cancer therapy and justify further clinical evaluation.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148264599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}